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Vaccine Detail

S. pneumoniae GtS Protein Vaccine
Vaccine Information
  • Vaccine Name: S. pneumoniae GtS Protein Vaccine
  • Target Pathogen: Streptococcus pneumoniae
  • Target Disease: Pneumonia
  • Vaccine Ontology ID: VO_0004068
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: recombinant (r) Glutamyl tRNA synthetase (GtS) (Mizrachi et al., 2007).
  • GltX gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant: aluminum vaccine adjuvant
  • Immunization Route: Intraperitoneal injection (i.p.)
Host Response

Mouse Response

  • Host Strain: BALB/c OlaHsd
  • Vaccination Protocol: Mice were immunized intraperitoneally with 25 μg of rGtS and 75 μL of Imject Alum adjuvant on days 0 and 21. Control mice were sham immunized with adjuvant only (Mizrachi et al., 2007).
  • Challenge Protocol: For lethal respiratory challenge with S. pneumoniae, rGtS‐immunized and control mice were anesthetized with isoflurane and inoculated intranasally with S. pneumoniae serotype 3 strain WU2 (in 25 μL of PBS). The size of this inoculum was found to be the lowest that causes 100% mortality in control mice within 96 h. Survival was monitored daily (Mizrachi et al., 2007).
  • Efficacy: Thirty-nine percent of rGtS-immunized mice survived a lethal bacterial challenge, whereas no control mice survived (Mizrachi et al., 2007).
References
Mizrachi et al., 2007: Mizrachi Nebenzahl Y, Bernstein A, Portnoi M, Shagan M, Rom S, Porgador A, Dagan R. Streptococcus pneumoniae surface-exposed glutamyl tRNA synthetase, a putative adhesin, is able to induce a partially protective immune response in mice. The Journal of infectious diseases. 2007; 196(6); 945-953. [PubMed: 17703427].