• Vaccine Name: S. pneumoniae 6PGD Protein Vaccine
• Target Pathogen: Streptococcus pneumoniae
• Target Disease: Pneumonia
• Vaccine Ontology ID: VO_0004069
• Type: Subunit vaccine
• Status: Research
• Antigen: Recombinant 6-Phosphogluconate dehydrogenase (6PGD) (Daniely et al., 2006).
• Gnd gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000884
  • Description: Inject Alum adjuvant (Daniely et al., 2006).
• Immunization Route: Intraperitoneal injection (i.p.)

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Six-week-old BALB/c female mice were immunized intraperitoneally with 25 µg of r6PGD and 75 µl of Inject Alum adjuvant on days 0 (primary immunization) and 21 (booster). Control mice were sham-immunized with adjuvant only (Daniely et al., 2006).
• Challenge Protocol: For respiratory challenge r6PGD immunized and control mice were anaesthetized with pentobarbital sodium (0·6 mg/kg) and inoculated intranasally with 1 × 10^8 S. pneumoniae strain WU2 (in 25 µl PBS). This inoculum's size was used as it was found to be the lowest that causes 100% mortality in our mouse model system within 96 h. Survival was monitored daily (Daniely et al., 2006).
• Efficacy: Immunization of mice with r6PGD protected 60% (P < 0.001) of mice for 5 days and 40% (P < 0.05) of the mice for 21 days following intranasal lethal challenge (Daniely et al., 2006).