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Vaccine Detail

S. pneumoniae 6PGD Protein Vaccine
Vaccine Information
  • Vaccine Name: S. pneumoniae 6PGD Protein Vaccine
  • Target Pathogen: Streptococcus pneumoniae
  • Target Disease: Pneumonia
  • Vaccine Ontology ID: VO_0004069
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: Recombinant 6-Phosphogluconate dehydrogenase (6PGD) (Daniely et al., 2006).
  • Gnd gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant: aluminum vaccine adjuvant
  • Immunization Route: Intraperitoneal injection (i.p.)
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Six-week-old BALB/c female mice were immunized intraperitoneally with 25 µg of r6PGD and 75 µl of Inject Alum adjuvant on days 0 (primary immunization) and 21 (booster). Control mice were sham-immunized with adjuvant only (Daniely et al., 2006).
  • Challenge Protocol: For respiratory challenge r6PGD immunized and control mice were anaesthetized with pentobarbital sodium (0·6 mg/kg) and inoculated intranasally with 1 × 108 S. pneumoniae strain WU2 (in 25 µl PBS). This inoculum's size was used as it was found to be the lowest that causes 100% mortality in our mouse model system within 96 h. Survival was monitored daily (Daniely et al., 2006).
  • Efficacy: Immunization of mice with r6PGD protected 60% (P < 0.001) of mice for 5 days and 40% (P < 0.05) of the mice for 21 days following intranasal lethal challenge (Daniely et al., 2006).
References
Daniely et al., 2006: Daniely D, Portnoi M, Shagan M, Porgador A, Givon-Lavi N, Ling E, Dagan R, Mizrachi Nebenzahl Y. Pneumococcal 6-phosphogluconate-dehydrogenase, a putative adhesin, induces protective immune response in mice. Clinical and experimental immunology. 2006; 144(2); 254-263. [PubMed: 16634799].