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Vaccine Detail
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T. cruzi DNA Vaccine pGFP-TSA1 |
| Vaccine Information |
- Vaccine Name: T. cruzi DNA Vaccine pGFP-TSA1
- Target Pathogen: Trypanosoma cruzi
- Target Disease: Chagas disease
- Vaccine Ontology ID: VO_0001390
- Type: DNA vaccine
- Status: Research
- TS
gene engineering:
- Type: DNA vaccine construction
- Description:
- Detailed Gene Information: Click Here.
- DNA vaccine plasmid:
- DNA vaccine plasmid name:
- DNA vaccine plasmid VO ID: VO_0005069
- Immunization Route: Gene Gun
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| Host Response |
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Mouse Response
- Host Strain: C57BL/6
- Vaccination Protocol: B6 mice were immunized four times at two-week intervals with 6 μg of each plasmid using a Helios Gene Gun (BioRad, NY, USA) (Chou et al., 2008).
- Challenge Protocol: Two weeks after the last vaccination, mice were infected with 1000 blood-derived T. cruzi trypomastigotes by s.c. injection at the base of the tail. Parasitemia levels were evaluated by counting the number of parasites in 5 μl of blood from the tail vein (Chou et al., 2008).
- Efficacy: C57BL/6 mice vaccinated with this plasmid showed suppressed parasitemia and prolonged survival. Vaccination with pGFP-TSA1 enhanced epitope-specific cytotoxicity and IFN-gamma secretion by CD8(+)T cells (Chou et al., 2008).
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| References |
Chou et al., 2008: Chou B, Hisaeda H, Shen J, Duan X, Imai T, Tu L, Murata S, Tanaka K, Himeno K. Critical contribution of immunoproteasomes in the induction of protective immunity against Trypanosoma cruzi in mice vaccinated with a plasmid encoding a CTL epitope fused to green fluorescence protein. Microbes and infection / Institut Pasteur. 2008; 10(3); 241-250. [PubMed: 18321749].
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