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Vaccine Detail

TBEV DNA vaccine encoding E protein
Vaccine Information
  • Vaccine Name: TBEV DNA vaccine encoding E protein
  • Target Pathogen: Tick-borne Encephalitis Virus (TBEV)
  • Target Disease: Tick-borne Encephalitis
  • Vaccine Ontology ID: VO_0004154
  • Type: DNA vaccine
  • Status: Research
  • TBEVgp1 polyprotein gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pCMV-beta DNA vaccine plasmid
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Groups of 16 BALB/c mice each received primary and booster immunizations spaced 4 wk apart. Plasmids were purified and administered i.m. (100 µg/dose) or by particle bombardment with DNA-coated gold beads (2 µg/dose) using the helium-powered Helios Gene Gun delivery system. Intramuscular inoculation of DNA in saline was done as a 100-µl injection in the right quadriceps. For Gene Gun inoculation, 1.0 µg of the same DNA was coupled to 0.5 mg of 1.0-µm-diameter gold particles, as recommended by the manufacturer (Bio-Rad). DNA-coated microcarriers were delivered into the abdominal epidermis of mice using the Gene Gun at a helium pressure setting of 400 psi. Mice immunized with the commercial TBE vaccine received 0.2 ml s.c. inoculations containing 1 µg formalin-inactivated virus (FSME Immun Inject, Baxter-Immuno, Vienna, Austria) (Aberle et al., 1999).
  • Challenge Protocol: Mice were subsequently challenged by i.p. inoculation with 1000 LD50 of the highly mouse-pathogenic TBE virus strain Hypr. (Aberle et al., 1999).
  • Efficacy: The plasmid construct encoding a secreted subviral particle, which carries multiple copies of the protective Ag on its surface, was superior to the other constructs in terms of extent and functionality of the Ab response as well as protection against virus challenge (Aberle et al., 1999).
References
Aberle et al., 1999: Aberle JH, Aberle SW, Allison SL, Stiasny K, Ecker M, Mandl CW, Berger R, Heinz FX. A DNA immunization model study with constructs expressing the tick-borne encephalitis virus envelope protein E in different physical forms. Journal of immunology (Baltimore, Md. : 1950). 1999; 163(12); 6756-6761. [PubMed: 10586074].