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Vaccine Detail

C. jejuni DNA vaccine pcDNA3.1(+)-cadF
Vaccine Information
  • Vaccine Name: C. jejuni DNA vaccine pcDNA3.1(+)-cadF
  • Target Pathogen: Campylobacter jejuni
  • Target Disease: Campylobacterosis
  • Vaccine Ontology ID: VO_0011545
  • Type: DNA vaccine
  • Status: Research
  • Antigen: C. jejuni fibronectin binding protein cadF
  • cadF gene engineering:
    • Type: DNA vaccine construction
    • Description: The chitosan-DNA vaccines was prepared by embedding pcDNA3.1(+)-cadF with chitosan (Zheng et al., 2007).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pcDNA3.1 DNA vaccine plasmid
  • Immunization Route: Intranasal
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: BALB/c mice were intranasally immunized in a four-dose primary series (7 d intervals) at doses of 60 microg chitosan-DNA vaccines each time (Zheng et al., 2007).
  • Challenge Protocol: Mice were attacked repeatedly through intragastric administration of C. jejuni HS:19 at the 8th week after the immunization and protective efficacy was determined by detecting the degrees of protection afforded against C. jejuni invaded (Zheng et al., 2007).
  • Efficacy: The mice immunized with chitosan-DNA vaccines have generated high levels of IgA and IgG from the sera and IgA from the intestinal secretions and the P/N value went up to 20.58, 30.13 and 6.87 respectively. Moreover the chitosan-DNA vaccines induced strongest level of protection in BALB/c mice against challenge with C. jejuni HS:19 strain and the protective efficacies was 93.70. The results of this study indicate that the chitosan-DNA vaccines could induce significant protective immunity against C. jejuni challenge in the mice model (Zheng et al., 2007).
References
Zheng et al., 2007: Zheng H, Cai FC, Zhong M, Deng B, Li X, Zhang XP. [Experimental study on the chitosan-DNA vaccines against campylobacter jejuni invasion]. Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]. 2007; 41(5); 375-379. [PubMed: 18206008].