• Vaccine Name: C. jejuni FlaC protein vaccine
• Target Pathogen: Campylobacter jejuni
• Target Disease: Campylobacterosis
• Vaccine Ontology ID: VO_0011495
• Type: Subunit vaccine
• Status: Research
• Antigen: C. jejuni flagellin subunit protein FlaC
• FlaC gene engineering:
  • Type: Recombinant protein preparation
  • Description: The flaC gene from C. jejuni 81-176 was expressed in Escherichia coli as hexahistidine-tagged proteins in pET-19b. Strains of BL21(DE3) containing each clone were grown in Luria broth containing 100 μg/ml ampicillin and proteins were purified by nickel chromatography under native conditions (Baqar et al., 2008).
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001321
• Immunization Route: Intranasal

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Mice were lightly anesthetized with isoflurane, and 30 μl of phosphate-buffered saline (PBS) alone or PBS containing 5 μg (7 to 10 mice per group) or 25 or 100 μg (10 to 18 mice per group) of protein was applied a drop (5 to 6 μl) at a time to the external nares. A total of three vaccinations for each dose level of each protein were delivered at 2-week intervals. To determine efficacy, animals receiving 100 μg of each protein alone or with 1 μg of LTR192G as the adjuvant were challenged with homologous or heterologous strains of C. jejuni. Following vaccination, animals were observed for two consecutive days for the development of vaccine-associated side effects (Baqar et al., 2008).
• Challenge Protocol: Twenty-eight days following the last vaccination, mice were intranasally challenged with 3 × 10^9 CFU of C. jejuni 81-176 or CG8486 (Baqar et al., 2008).
• Efficacy: FlaC provided an 18% protection against disease from C. jejuni 81-176 (Baqar et al., 2008).