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Vaccine Detail

Dengue Subunit E Protein Vaccine
Vaccine Information
  • Vaccine Name: Dengue Subunit E Protein Vaccine
  • Target Pathogen: Dengue Virus
  • Target Disease: Dengue Fever
  • Vaccine Ontology ID: VO_0011457
  • Type: Subunit vaccine
  • Status: Research
  • E protein gene engineering:
    • Type: Recombinant protein preparation
    • Description: Baculovirus was used to generate the recombinant protein (Delenda et al., 1994).
    • Detailed Gene Information: Click Here.
  • Adjuvant: complete Freunds adjuvant
  • Adjuvant: incomplete Freunds adjuvant
  • Immunization Route: Intraperitoneal injection (i.p.)
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Three-week-old female BALB/c mice were immunized by intraperitoneal injection on days 1,7, 21 and 56 with 200 gl of baculovirus infected Sf9 cell lysates (CL) or concentrated supernatant fluids (SF) supplemented with complete Freund adjuvant for the first and with incomplete Freund adjuvant for the three other injections.
  • Challenge Protocol: Mice were challenged withDEN-2 virus (Delenda et al., 1994).
  • Efficacy: Sixty eight percent (P < 0.001) of mice vaccinated with 5 gg of extracellular D2EA102 protein were protected against lethal challenge (Delenda et al., 1994).
References
Delenda et al., 1994: Delenda C, Frenkiel MP, Deubel V. Protective efficacy in mice of a secreted form of recombinant dengue-2 virus envelope protein produced in baculovirus infected insect cells. Archives of virology. 1994; 139(1-2); 197-207. [PubMed: 7826210].