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Vaccine Detail

T. gondii DNA vaccine pcDNA3-MIC3
Vaccine Information
  • Vaccine Name: T. gondii DNA vaccine pcDNA3-MIC3
  • Target Pathogen: Toxoplasma gondii
  • Target Disease: Toxoplasmosis
  • Vaccine Ontology ID: VO_0011523
  • Type: DNA vaccine
  • Status: Research
  • MIC3 gene engineering:
    • Type: DNA vaccine construction
    • Description: Protein MIC3 (Xiang et al., 2009).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pcDNA3 DNA vaccine plasmid
  • Immunization Route: Footpad injection
Host Response

Mouse Response

  • Host Strain: Kunming
  • Vaccination Protocol: All studies were done with 6–8 weeks old Kunming mice. For experimental group, 100 μg pcDNA3-MIC3 were injected into mouse hind footpad three times, at week 0, 3 and 6, respectively. The mice of control groups were vaccinated with 100 μg pcDNA3 or normal saline (NS) three times (Xiang et al., 2009).
  • Challenge Protocol: Immunized Kunming mice were challenged intraperitoneally with 500 tachyzoites of RH strain T. gondii 9 weeks after the first immunization (Xiang et al., 2009).
  • Efficacy: The survival time of mice in pcDNA3-MIC3 group was significantly longer than those of in group pcDNA3 and NS (Xiang et al., 2009).
References
Xiang et al., 2009: Xiang W, Qiong Z, Li-peng L, Kui T, Jian-wu G, Heng-ping S. The location of invasion-related protein MIC3 of Toxoplasma gondii and protective effect of its DNA vaccine in mice. Veterinary parasitology. 2009; 166(1-2); 1-7. [PubMed: 19800170].