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Vaccine Detail

WEEV DNA Vaccine encoding 6K-E1 Protein
Vaccine Information
  • Vaccine Name: WEEV DNA Vaccine encoding 6K-E1 Protein
  • Target Pathogen: Western equine encephalomyelitis virus
  • Target Disease: Western equine encephalomyelitis (WEE)
  • Vaccine Ontology ID: VO_0011505
  • Type: DNA vaccine
  • Status: Research
  • E1 gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • 6K gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pVAX DNA vaccine plasmid
  • Immunization Route: Gene Gun
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Three doses of the DNA vaccine containing 2 μg of plasmid DNA in each vaccine were administered 14 days apart by using a Helios gene gun. Inactivated WEE vaccine was used as a positive control (Gauci et al., 2010).
  • Challenge Protocol: At 14 days after the third vaccination, the mice were challenged intranasally with 1,500 PFU (25 50% lethal doses [LD50s]) of the homologous 71V-1658 strain of WEEV, 1,500 PFU (25 LD50s) of the heterologous Fleming strain, or 1,500 PFU of the heterologous CBA87 strain. The challenged mice were observed for 14 days for survival and the severity of the infection (Gauci et al., 2010).
  • Efficacy: The 6K-E1 structural protein encoded by the DNA vaccine conferred complete protection against challenge with the homologous strain and limited protection against challenge with a heterologous strain (Gauci et al., 2010).
References
Gauci et al., 2010: Gauci PJ, Wu JQ, Rayner GA, Barabé ND, Nagata LP, Proll DF. Identification of Western equine encephalitis virus structural proteins that confer protection after DNA vaccination. Clinical and vaccine immunology : CVI. 2010; 17(1); 176-179. [PubMed: 19923571].