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Vaccine Detail

E. tenella GX3262 protein vaccine
Vaccine Information
  • Vaccine Name: E. tenella GX3262 protein vaccine
  • Target Pathogen: Eimeria tenella
  • Target Disease: Hemorrhagic cecal coccidiosis
  • Vaccine Ontology ID: VO_0011470
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: E. tenella sporozoite antigen GX3262
  • GX3262 gene engineering:
    • Type: Recombinant protein preparation
    • Description: To produce the 3-galactosidase-GX3262 fusion protein (Pgal-GX3262), recombinant E. coli cells. grown overnight in Luria-Bertani broth with ampicillin and induced with IPTG, were lysed by sonication and centrifuged, and the insoluble cell pellet was washed extensively with PBS (Miller et al., 1989).
    • Detailed Gene Information: Click Here.
  • Adjuvant: complete Freunds adjuvant
  • Immunization Route: Subcutaneous injection
Host Response

Chicken Response

  • Host Strain: Hubbard broiler
  • Vaccination Protocol: The basic experimental design involved immunizing birds subcutaneously or orally with partially purified recombinant antigen preparations, heatkilled bacterins. live E. coli cells, or control materials (Miller et al., 1989).
  • Challenge Protocol: Broilers were challenged with E. tendell/i oocysts; and then evaluated for cecal lesions (Miller et al., 1989).
  • Efficacy: A cDNA clone derived from sporulated oocysts of Eimeria tenella and encoding the expression product GX3262 was identified using a monoclonal antibody raised against Eimeria acervulina sporozoites. After challenge with an experimental E. tenella infection, the greatest degree of protection in immunized broiler chickens was seen after only a single immunization of 2-day-old birds with a live recombinant E. coli preparation (Miller et al., 1989).
References
Miller et al., 1989: Miller GA, Bhogal BS, McCandliss R, Strausberg RL, Jessee EJ, Anderson AC, Fuchs CK, Nagle J, Likel MH, Strasser JM. Characterization and vaccine potential of a novel recombinant coccidial antigen. Infection and immunity. 1989; 57(7); 2014-2020. [PubMed: 2659532].