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Vaccine Detail

E. histolytica CEL-170/4 protein vaccine
Vaccine Information
  • Vaccine Name: E. histolytica CEL-170/4 protein vaccine
  • Target Pathogen: Entamoeba histolytica
  • Target Disease: Amoebiasis
  • Vaccine Ontology ID: VO_0011414
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: E. histolytica alactose-specific adherence lectin heavy subunit (CEL-170/4)
  • CEL-170/4 gene engineering:
    • Type: Recombinant protein preparation
    • Description: The galactose-specific lectin has been purified from a pathogenic strain of E. histolytica by monoclonal antibody affinity chromatography (Petri and Ravdin, 1991).
    • Detailed Gene Information: Click Here.
  • Adjuvant: complete Freunds adjuvant
  • Adjuvant: incomplete Freunds adjuvant
  • Immunization Route: Intraperitoneal injection (i.p.)
Host Response

Mongolian Gerbil Response

  • Vaccination Protocol: Adult male gerbils were immunized by intraperitoneal or subcutaneous injection of 10 ,ug of the affinity-purified lectin emulsified in complete Freund's adjuvant (GIBCO, Grand Island, N.Y.) and boosted with 10 ,ug of the lectin in incomplete Freund's adjuvant at 2 and 4 weeks. Control gerbils were sham immunized with Freund's complete and incomplete adjuvants alone. Prechallenge gerbil sera were collected at 5 weeks after the initial immunization by cardiac puncture (Petri and Ravdin, 1991).
  • Challenge Protocol: Gerbils were challenged intrahepatically with E. histolytica (Petri and Ravdin, 1991).
  • Efficacy: Researcherse tested the ability of the galactose-specific adherence lectin of E. histolytica to elicit a protective immune response in conjunction with Freund's incomplete and complete adjuvants. Four independent trials demonstrated complete protection from amebic liver abscess formation in 67% of lectin-immunized gerbils. The gerbil antilectin antibodies were shown by Western immunoblotting to be directed to the heavy subunit (CEL-170/4) but not the light subunit of the lectin (Petri and Ravdin, 1991).
References
Petri and Ravdin, 1991: Petri WA Jr, Ravdin JI. Protection of gerbils from amebic liver abscess by immunization with the galactose-specific adherence lectin of Entamoeba histolytica. Infection and immunity. 1991; 59(1); 97-9101. [PubMed: 1987067].