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Vaccine Detail

H. pylori vaccine encoding UreB
Vaccine Information
  • Vaccine Name: H. pylori vaccine encoding UreB
  • Target Pathogen: Helicobacter pylori
  • Target Disease: Ulcers
  • Vaccine Ontology ID: VO_0011477
  • Type: Recombinant vector vaccine
  • Status: Research
  • Antigen: H. pylori urease subunit B (ureB)
  • ureB gene engineering:
    • Type: Recombinant vector construction
    • Description: UreB replicon was constructed, encapsidated and produced as previously described in detail. Briefly, the plasmid pHP 902 containing the entire gene for the urease B subunit of a type 2 H. pylori (UMAB 41) was kindly provided by HL Mobley, University of Maryland. The urease gene was amplified by PCR using DNA primers with unique Xhol (5′) and HpaI (3′) restriction sites. The DNA product was cloned into the plasmid pCRII (Invirogen, San Diego, CA), digested with Xhol and HpaI, and ligated into a replicon cDNA. A schematic representation of the replicon encoding the B subunit of H. pylori urease (Smythies et al., 2005).
    • Detailed Gene Information: Click Here.
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Host Strain: C57BL/6/DAB
  • Vaccination Protocol: C57BL/6/DAB TgPVR mice were vaccinated intramuscularly with 107 infectious units of UreB replicon or control L1 replicon (5 mice each in Experiment 1 and 10 mice each in Experiment 2) and then boosted with UreB replicon or L1 replicon, respectively, 1 and 2 weeks later. Animals were immunized intramuscularly to assure delivery of equivalent amounts of vaccine to each animal and because poliovirus receptor transgenic mice are not susceptible to poliovirus infection by oral inoculation, even when the poliovirus receptor is overexpressed in the intestinal epithelium. Two weeks after the final boost, animals were inoculated with H. pylori SPM326 (150 μl, 109 CFU/ml) by oral gavage four times during a 10-day period with at least 3 days between each gavage (Smythies et al., 2005).
  • Challenge Protocol: Mice were challenged with H. pylori (Smythies et al., 2005).
  • Efficacy: Vaccination with poliovirus vector containing the gene for the B subunit of H. pylori urease provides significant prophylactic and strong therapeutic protection against H. pylori in mice (Smythies et al., 2005).
References
Smythies et al., 2005: Smythies LE, Novak MJ, Waites KB, Lindsey JR, Morrow CD, Smith PD. Poliovirus replicons encoding the B subunit of Helicobacter pylori urease protect mice against H. pylori infection. Vaccine. 2005; 23(7); 901-909. [PubMed: 15603891].