• Vaccine Name: Herpes simplex virus type 2 DNA vaccine encoding ICP27
• Target Pathogen: Herpes simplex virus type 1 and 2
• Target Disease: Herpes
• Vaccine Ontology ID: VO_0011405
• Type: DNA vaccine
• Status: Research
• Antigen: Herpes simplex virus type 2 transcriptional regulator ICP27
• ICP27 from Herpes simplex virus type 2 gene engineering:
  • Type: DNA vaccine construction
  • Description: The plasmid-encoding HSV-2 ICP27 was generated by inserting a PCR fragment incorporating the entire ICP27 gene (UL54) into pTarget (Promega, Madison, WI) (Haynes et al., 2006).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000318

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Each immunization of 6–8-week-old female Balb/c mice consisted of a single PMED™ delivery to the ventral abdominal skin in which each delivery contained 0.5 mg of gold coated with a total of 0.5 μg of the DNA vaccine-DEI vector formulation. Two such “single shot” immunizations were administered 4 weeks apart and animals were sacrificed or challenged 2 weeks following the second immunization (Haynes et al., 2006).
• Challenge Protocol: Anesthetized Balb/c mice were intranasally challenged with 30 μl of PBS containing approximately 50 LD50 (2 × 10^6 PFU) of HSV-2 strain MS. Mice were followed for 20 days after infection and scored for morbidity and mortality (Haynes et al., 2006).
• Efficacy: Particle-mediated DNA vaccination of mice with a DNA plasmid-encoding ICP27 resulted in the induction of ICP27-specific IFN-gamma and TNF-alpha production in Balb/c mice. When the DNA vaccine was supplemented with as little as 50ng of a vector encoding the A and B subunits of the Escherichia coli heat labile enterotoxin (LT), animals were profoundly protected from morbidity and mortality (Haynes et al., 2006).