• Vaccine Name: PCV2 DNA Vaccine encoding ORF2 Protein
• Target Pathogen: Porcine circovirus 2
• Target Disease: Postweaning multisystemic wasting syndrome
• Vaccine Ontology ID: VO_0011481
• Type: DNA vaccine
• Status: Research
• ORF2 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000214
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: The mice in the vaccine groups were injected intramuscularly in the quadriceps with 100 µg pORF2 plasmid prepared in 100 µl PBS as follows. The mice in the pORF2 group received vaccination with pORF2 plasmid, three times every 2 weeks, whilst those in the pORF2/Cap or Cap/pORF2 group were primed with pORF2 plasmid (or Cap protein) and boosted with doses of Cap protein (or pORF2 plasmid) twice every 2 weeks (Shen et al., 2008).
• Challenge Protocol: At 16 weeks p.i., the mice were challenged intraperitoneally with 0.2 ml PCV2 inoculum (105.75 TCID50 1/ml) (Shen et al., 2008).
• Efficacy: Following virus challenge, real-time PCR and histopathological analysis confirmed that only low viral DNA loads and mild microscopic lesions appeared in pORF2-immunized mice (Shen et al., 2008).
• Host Ighg1 response
  • Description: Serum IgG1 was significantly higher in mice immunized with PCV2 DNA vaccine encoding ORF2 protein than control mice, vaccinated with the pCI-neo vector and crude lysate of E. coli strain BL21 transformed with pGEX-4T-1 were used as substitutes for pORF2 plasmid and Cap protein, respectively. The up regulation began 4 weeks after immunization (Shen et al., 2008).
  • Detailed Gene Information: Click Here.
• Host Ighv1-9 response
  • Description: Serum IgG2a was significantly higher in mice immunized with PCV2 DNA vaccine encoding ORF2 protein than control mice, vaccinated with the pCI-neo vector and crude lysate of E. coli strain BL21 transformed with pGEX-4T-1 were used as substitutes for pORF2 plasmid and Cap protein, respectively. The up regulation began 4 weeks after immunization (Shen et al., 2008).
  • Detailed Gene Information: Click Here.