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Vaccine Detail

IHNV DNA vaccine pIHNw-G
Vaccine Information
  • Vaccine Name: IHNV DNA vaccine pIHNw-G
  • Target Pathogen: Infectious Hematopoietic Necrosis Virus
  • Target Disease: Infectious hematopoietic necrosis
  • Vaccine Ontology ID: VO_0011398
  • Type: DNA vaccine
  • Status: Research
  • Antigen: Infectious Hematopoietic Necrosis Virus glycoprotein antigen
  • G glycoprotein gene engineering:
    • Type: DNA vaccine construction
    • Description: The open reading frame of the G gene of IHNV WRAC strain was amplifed by reverse transcriptase-polymerase chain reaction using sequence-specic primers and cloned into the pT7-blue vector (Novagen, Madison, WI). The gene was then subcloned downstream of the immediate-early enhancer-promoter sequences of human cytomegalovirus (CMV) of the pCDNA 3.1 vector (Invitrogen). The resulting plasmid was designated pIHNw-G (previously pCDNA-G) (Corbeil et al., 2000).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pcDNA3.1 DNA vaccine plasmid
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Rainbow trout Response

  • Vaccination Protocol: Fish from cohort 1 were used for viral challenge experiments, serological analyses, histology, and passive transfer experiments 1 and 2. For these studies two groups of 500 juvenile rainbow trout from cohort 1 (mean weight 2.5 g) were anaesthetized by immersion in 100 μg/ml tricaine methane sulfonate (MS-222; Argent Chemical Laboratories, Redmond, WA) and vaccinated by i.m. injection with 0.1 μg of either pIHNw-G or pLuc in a total volume of 50 μl phosphate buffered saline (PBS). A group of 200 fish was left unhandled, and all fish were maintained at 12 °C for the entire study period (Kurath et al., 2006).
  • Challenge Protocol: . At 25 months post-vaccination a challenge experiment was done with three subgroups of 18 fish per treatment. For each experiment duplicate groups of pIHNw-G vaccinated fish were i.p. injected with IHNV as described above, and the third group was injected with 200 μl MEM without virus. Similarly, two groups of pLuc vaccinated fish were challenged with IHNV, and the third group was mock-challenged (Kurath et al., 2006).
  • Efficacy: The DNA vaccine pIHNw-G encodes the glycoprotein of the fish rhabdovirus infectious hematopoietic necrosis virus (IHNV). Vaccine performance in rainbow trout was measured 3, 6, 13, 24, and 25 months after vaccination. At three months all fish vaccinated with 0.1 microg pIHNw-G had detectable neutralizing antibody (NAb) and they were completely protected from lethal IHNV challenge with a relative percent survival (RPS) of 100% compared to control fish (Kurath et al., 2006).
References
Corbeil et al., 2000: Corbeil S, LaPatra SE, Anderson ED, Kurath G. Nanogram quantities of a DNA vaccine protect rainbow trout fry against heterologous strains of infectious hematopoietic necrosis virus. Vaccine. 2000; 18(25); 2817-2824. [PubMed: 10812224].
Kurath et al., 2006: Kurath G, Garver KA, Corbeil S, Elliott DG, Anderson ED, LaPatra SE. Protective immunity and lack of histopathological damage two years after DNA vaccination against infectious hematopoietic necrosis virus in trout. Vaccine. 2006; 24(3); 345-354. [PubMed: 16154239].