• Vaccine Name: Rift Valley Fever Virus DNA Vaccine encoding N Protein
• Target Pathogen: Rift Valley Fever virus
• Target Disease: Rift valley fever
• Vaccine Ontology ID: VO_0011399
• Type: DNA vaccine
• Status: Research
• RVFVsSgp2 N protein gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005062
• Immunization Route: Gene Gun

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Before immunisation the mice were thoroughly shaved on the abdomen and vaccinated with cDNA encoding the antigens using a gene-gun (Helios™, BioRad Laboratories). The cDNA was administrated four times with two to three week intervals. The primary immunisation was performed using four gene-gun cartridges and the following three boosters with two cartridges (Lagerqvist et al., 2009).
• Challenge Protocol: Mice were injected intraperitoneally (i.p.) with RVFV diluted in sterile PBS to a final volume of 100 μl (Lagerqvist et al., 2009).
• Efficacy: Even though complete protection was not achieved by genetic immunisation, four out of eight mice vaccinated with cDNA encoding the nucleocapsid protein displayed no clinical signs of infection after challenge. In contrast, all fourteen control animals displayed clinical manifestations of Rift Valley Fever after challenge (Lagerqvist et al., 2009).