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Brucella abortus RB51 vaccine vector |
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Vaxvec ID |
13 |
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Vaccine Vector Name |
Brucella abortus RB51 vaccine vector |
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Vector VO ID |
VO_0000020
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Function |
RB51 is able to stimulate strong cell-mediated immunity (He et al., 2001). |
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Advantage |
RB51 is able to induce strong CD4+ and CD8+ T-cell mediated immune responses (He et al., 2001). |
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Disadvantage |
Other so many DNA plasmids are able to survive inside the RB51 bacteria. Also, known DNA plasmids able to survive and replicate inside RB51 tend not to have high copy number. |
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Preparation |
RB51 was derived by repeated passage of virulent B. abortus strain 2308 on Trypticase soy supplemented with 1.5% agar and varying concentrations rifampin or penicillin (Schurig et al., 1991). |
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Storage |
Usually at 2-8 degree. |
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Host |
12 |
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Safety |
While RB51 is licensed for cattle use, it cannot be used for human due to its capability of potentially causing human infection. So the safety concern is a main disadvantage for its use. However, since it is a licensed cattle vaccine, it is very suitable to express protective antigens in other pathogens as a bacterial vaccine vector. Such a vaccine would protect the cattle against brucellosis and the other pathogen infection. |
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Stability |
The stability of RB51 as a vaccine vector is high. |
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Description |
RB51 is a live attenuated cattle brucellosis vaccine strain that has been licensed for cattle use in the USA and many other countries. This B. abortus strain has been experimentally verified to be a bacterial vaccine vector (Vemulapalli et al., 2007). |
| Related Vaccine(s) |
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| References |
He et al., 2001: He Y, Vemulapalli R, Zeytun A, Schurig GG. Induction of specific cytotoxic lymphocytes in mice vaccinated with Brucella abortus RB51. Infection and immunity. 2001; 69(9); 5502-5508. [PubMed: 11500423].
Schurig et al., 1991: Schurig GG, Roop RM 2nd, Bagchi T, Boyle S, Buhrman D, Sriranganathan N. Biological properties of RB51; a stable rough strain of Brucella abortus. Veterinary microbiology. 1991; 28(2); 171-188. [PubMed: 1908158].
Vemulapalli et al., 2007: Vemulapalli R, Sanakkayala N, Gulani J, Schurig GG, Boyle SM, Lindsay DS, Sriranganathan N. Reduced cerebral infection of Neospora caninum in BALB/c mice vaccinated with recombinant Brucella abortus RB51 strains expressing N. caninum SRS2 and GRA7 proteins. Veterinary parasitology. 2007; 148(3-4); 219-230. [PubMed: 17651896].
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