Chlamydia muridarum 83560 Chlamydia muridarum is an intracellular bacterial species that at one time belonged to Chlamydia trachomatis. However, C. trachomatis naturally only infects humans and C. muridarum naturally infects only members of the family Muridae (includes both mice and hamsters, Alderton, 1996). Two strains of Chlamydia muridarum, MoPn (originally named Nigg) and SFPD, have been isolated from mice and hamsters. Glycogen production by both strains has been demonstrated, although it is difficult to detect glycogen in MoPn (Moulder et al., 1984). Chlamydia muridarum MoPn binds mAbs recognizing Chlamydia trachomatis MOMP vs4 core epitope (T)LNPT(IA). DNA sequence analysis indicates that these mAbs should recognize SFPD and that Chlamydia trachomatis B-serogroup mAbs specific for the vs4 epitope IAGAG should recognize SFPD (Wiki: Chlamydia muridarum). Cattle Bos taurus 9913 Chicken Gallus gallus 9031 chinchillas Chinchillidae 10150 Copper Pheasant Syrmaticus soemmerringii 9067 Deer mouse Peromyscus maniculatus 10042 Ducks Anas 8835 Ferret Mustela putorius furo 9669 Goat Capra hircus 9925 Guinea pig Cavia porcellus 10141 Hamster Mesocricetus auratus 10036 Horse Equus caballus 9796 Human Homo sapiens 9606 Monkey Platyrrhini 9479 Mouse Mus musculus 10090 Pig Sus scrofa 9823 Rabbit Oryctolagus cuniculus 9986 Rat Rattus 10114 Raven Corvus corax 56781 Sheep Ovis aries 9940 Vole Microtus ochrogaster 79684 C. muridarum DNA vaccine encoding TC0439 VO_0011533 Research Subcutaneous Subcutaneous DNA vaccine construction The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007). BALB/c Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007). The groups of female BALB/c mice immunized intra-abdominally with TC0439 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007). All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007). C. muridarum DNA vaccine encoding TC0512 VO_0011534 Research Subcutaneous injection pCI30 Subcutaneous injection DNA vaccine construction The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007). BALB/c Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007). The groups of female BALB/c mice immunized intra-abdominally with TC0512 showed significant levels of protection when compared to negative control animals. At 9 days following challenge TC0512 showed a 73% reduction in the number of recoverable Chlamydia compared with vector only immunized controls (McNeilly et al., 2007). All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007). C. muridarum DNA vaccine encoding TC0757 VO_0011522 Research Subcutaneous injection Subcutaneous injection DNA vaccine construction The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007). BALB/c Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007). The groups of female BALB/c mice immunized intra-abdominally with TC0757 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007). All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007). C. muridarum DNA vaccine encoding TC0767/768 VO_0011524 Research intra-abdominal injection intra-abdominal injection DNA vaccine construction The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007). DNA vaccine construction The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007). BALB/c Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007). The groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007). All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007). C. muridarum MOMP protein vaccine VO_0011453 Research Intranasal immunization Cholera toxin and CpG oligodeoxynucleotide Intranasal immunization Recombinant protein preparation Recombinant maltose binding protein-MOMP (MBP-MOMP) was cloned into the bacterial expression vector pMAL-c2, and used to transform Escherichia coli (DH5α[pMMM3]). The MBP-MOMP was purified by passage through a PD-10 column (Amersham Biosciences, NSW, Australia), followed by a Sephadex G-75 column (Sigma–Aldrich) (Skelding et al., 2006). BALB/c Groups of five mice were immunized on days 0, 7, 14, and 35 via either the IN or TCI route. One group of five mice were used as a control, receiving no immunization solution. Mice immunized intranasally were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia), and 10 μl of immunization solution containing 100 μg MOMP, admixed with 10 μg CpG-ODN 1826 (5′-TCC ATG ACG TTC CTG ACG TT-3′) (Geneworks, SA, Australia), and 10 μg cholera toxin (Sigma–Aldrich) was gently pipetted onto each nare (5 μl per nare) (Skelding et al., 2006). Both intranasal and transcutaneous immunization protected mice against respiratory challenge with Chlamydia (Skelding et al., 2006). Five days after the final boost, mice were infected intranasally with C. muridarum. Mice were anaesthetised with 4% isoflurane and 10^3 IFUs were gently pipetted onto the nares. Mice were weighed daily for 10 days then sacrificed 10 days post-infection via a sodium pentobarbital overdose (Nembutal, Abbott Australasia). The lungs from the mice were either perfused and fixed for histopathology or collected for determination of C. muridarum levels (Skelding et al., 2006). C. muridarum PmpE/F-2 protein vaccine VO_0011456 Research Intravenous injection (i.v.) Incomplete Freunds Adjuvant (Sigma) Intravenous injection (i.v.) Recombinant protein preparation The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: pmpE/F-2 and gap DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pET32a (Novagen) for pmpE/F-2 and gap after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques. Only the first half of the gene (25–575) was cloned into the vector for expression (Yu et al., 2009). C57BL/6 Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 10^6 DCs transfected with Chlamydia protein PmpE/F-225–575 in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009). PmpE/F-2(25-575) immunized mice exhibited significant resistance to challenge infection. In addition, pmpE/F-2 was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009). Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009). C. muridarum PmpG-1 protein vaccine VO_0011458 Research Intravenous injection (i.v.) Incomplete Freunds Adjuvant (Sigma) Intravenous injection (i.v.) Recombinant protein preparation The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: pmpG-1 and gap DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pET32a (Novagen) for pmpG-1 and gap after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques. Only the first half of the gene (25–500) was cloned into the vector for expression (Yu et al., 2009). C57BL/6 Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 10^6 DCs transfected with Chlamydia protein PmpG-1 25–500 in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009). Overall, vaccination with DCs transfected with PmpG-1(25-500) exhibited the greatest degree of protective immunity among the four Chlamydia Ags tested (Yu et al., 2009). Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009). C. muridarum RplF protein vaccine VO_0011437 Research Intravenous injection (i.v.) Incomplete Freunds Adjuvant (Sigma) Intravenous injection (i.v.) Recombinant protein preparation The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: rplF DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pGEX-6P-3 (GE Healthcare) after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques (Yu et al., 2009). C57BL/6 Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 10^6 DCs transfected with Chlamydia protein RplF in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009). RplF was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009). Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009). MOMP Chlamydia muridarum VO_0010890 1518659 CDD:144782 83560 ? major outer membrane protein 4.69 39600.904 387 mouse pneumonitis; submitted as Chlamydia trachomatis >gi|1518659|gb|AAB07068.1| major outer membrane protein [Chlamydia muridarum] MKKLLKSVLAFAVLGSASSLHALPVGNPAEPSLMIDGILWEGFGGDPCDPCTTWCDAISLRLGYYGDFVF DRVLKTDVNKQFEMGAAPTGDADLTTAPTPASRENPAYGKHMQDAEMFTNAAYMALNIWDRFDVFCTLGA TSGYLKGNSAAFNLVGLFGRDETAVAADDIPNVSLSQAVVELYTDTAFAWSVGARAALWECGCATLGASF QYAQSKPKVEELNVLCNAAEFTINKPKGYVGQEFPLNIKAGTVSATDTKDASIDYHEWQASLALSYRLNM FTPYIGVKWSRASFDADTIRIAQPKLETSILKMTTWNPTISGSGIDVDTKITDTLQIVSLQLNKMKSRKS CGLAIGTTIVDADKYAVTVETRLIDERAAHVNAQFRF Protective antigen Female BALB/c mice were immunized with chlamydial major outer membrane protein (MOMP) mixed with cholera toxin and CpG oligodeoxynucleotide adjuvants by either the IN or TCI routes. Both intranasal and transcutaneous immunization protected mice against respiratory challenge with Chlamydia [Ref1061:Skelding et al., 2006]. PmpE/F-2 Chlamydia muridarum Nigg VO_0010893 1246432 15834882 TC0262 NZ_ACOV01000003 NP_296641 243161 308276 311353 - polymorphic membrane protein E/F family protein 8.26 107782.38 1025 similar to GP:4376753; identified by sequence similarity; putative >gi|29337300:308276-311353 Chlamydia muridarum Nigg, complete genome GTTAAAAGACCAGAGCCCCTCCTGCATTCATGTAGTGTGAGACTGTAGAAGTAGCCACCTGCGCTTGATA ATTAGCGAATAGTTTAAGATAAGAGAATTTTAGAGAAGATGATCCTCTCCCATAAAAGGAATGCTTAGCT AAAGGAGTGTTTGTTGTTTCCCAAGAACCATTGTTTTTGATTAAAACGGTATTAAGAATGGGGCGCTTCC AGTAAAGAGTAGGCTGGTATGCTACCTCCATTTCGCAGGATACAGTTGGCCATTTATTCGAAGAATAAAC ACCTTTAATTCCTATTGGAGAAACAACAGCTGTATGTGGTTGTTTTAACGAAAATAATCTAGCCAGGTCT CCCTGCTCCTGGATAGATGCAGGCTCTGTTCGGGAGATGAGAGCTTGAATGAATGGTGTCAACATAATCG ATTGGAAAGGCATACTATCCCGCAGTTCGCAACGTAGAGAGCCCCCCAGAGTCGTAGAGTGAAAGTTCCC TTTAGAAAACTTTTTCGTTCCATAGAAGTTATATGTTTTATGAGTACCATAGTTATAAGCAGCTGCTCCA ATAAGAGCGATACGATCAAACATAGGTTGTTCAAAACACAGAGCGGAGAGATAATAACGAGAAGAAATTT TATTATTGGTACGTTTCTCTTTCATTGTGTCGGAGGATTGGGAAAAGGAAAATAAAAACTTATGTCCGTG TGCTCCTGATGCTTGTGAAGATATAGCATATCCTTTCGATGCAGAAGAATATCCGTGATAATCTAAGCGT TTCTGCTGTTTGGTGAATAGCGTAGCTGCAGATCCTTGTAAAGCGAACCAAGAGTCATCTGGTAGTAATG AACGTAAGTACAGGGTTGTATTTCTTCCAGAAGACACTAAATTATTCGCAATAAGATCCCCTCTACGAGC AGGATCTGCTATATATCCGATTGGGGTCCAGCTCACTACTAAGGAGTGTCTAGTGACTGTTACGTCCGTT GTAGCAAGAGGAGTTGTTCCTCCTGTAGAAGCTCTAATGGAAGGAATCTTAGCTGTAGTTGTACTGTTGG AATCAAAAGTTTCGCTAGTGTTTTTTTGTTCTTGAATAGAATTGTTGGCTGTTTGAGTCTCTGTACTATT GGTAAGAGAGACCGTACGGGTGGTTGTCATCCAATTAGATGTCCAGAGTCCTTGGTATCCGTAATGTGGT AAAGAATATAGCGAGGTATTGAGAGTGTTTTTCCAATCATTGCTTAACTGCGCATCATTAGTCGTTTCGA TATGTAAAATGGGTTGATTTGCTTTTCCTCGGGATAGGTCGCAGCTGTCATAAACTGATTCGTAATTTTC ATCTACTAACGAGACAAGACCGGAGAAAAAGATCTTTTGAGTTGCCACGTTCTCTGTGCTTACTGGAGCT TGGTTAGAGTTTGTATTCTGTGTCATATCTTGGGGATTAACACGAATACGTGGAGCTGTAGTACCTTGGT CATTGAGAAGACCTGGCAAAATGATTCCAAGATGCGTAATAGTGAGAGAAGCGTCTTTCCCTACAGTAAG AATAGAATCTTGTCCTAAAGCAAGAAGGCTAGTGCTTTCTTGAGTAAGAGGGCCATTGAAAAATTCAAAC TCTGCGCCATTTTCTATGGACATAGTCCCAAACTTCAAGGTAGTGGGGGAGTCTTTAATTTGGTTATGAG TTTTACTGGCAATCATGTGAGCTTGTAGATCACTGGATATGTTTTTGTATGAAAACACAACAGCGCCAGA GAACTCATCCGAAGGATTGATGGTAACGGTGTGCGGGCTATTTTCAGGGGGTGTATTAGCTTTTTTTAAG CCTCCCCATTGAAAAAGATCATAAAAATAGATGCGAGTATCTTTCTTAGCACCAAGAGATAGGGTGATAG GGTTATTGTTGGTTGTTCCAGGAATATAAAAAAGTTTTCGCAAACCTGCTGACATTCCGGTAGTTGAAAG CTCTGTATTATTGTTGAAGATAATATCCCCAGAGCCAGTATGAATATAGTTCGCAGCTGGGCTTGCAAAT AGGTGAAAAGCTCCTCCATACAGAGCCGAATTATTAAGAAAATAGATAGGACCATTTTCTTGAAAATTAA GGTTGTTGCAGGAAATAGCTCCTGCTATGTATGAGGCATTATTATTCTCAAAGTAGATAGGGCCTTTATT ATTTAGAAGACCAAACTCTCCTCCTGCGCTTATAACTGCTCCATTTCCTTGAGCACTAGTGTTATGCCAT CCTAAAGCGCTATTATTTATAAAAATAAGCTTATCCTTATTATCTCTAAAAATACACTGTCCCCCGGTGC TGATAGCTCCTCCGTATCCTCCATAGTTGGAATTGAAGACTACCGGACCAGTATTGTGTGAGATTTCGCA GGCACCTTGGCAGCCAACAGCTCCTCCTCGAGCAAGAATTGTAGCAAATGTGTTGGGTATGTGATCAGTA GTATGGTTATTTTCAAAAGCAACTATTCCGGACGTATTTTTGATAACTAGGTTTTGGATTGTGGATACGG CTCCTCCCATATCCCCAGAAGTGTTATTGATAAAATTTAAGCTATGATTATTAATTAGCTCGATGCTTGA TCCGTATAAAGCCCCTCCGCGATTCCTTTCGGTTCTTAATGCTACTCCTGTAAATTGAGTTCCAATAGTT GTATTATTATAGAACAATATGTTTTGGTTGTTTTTAAAGCTAATAAGGCCTGTAGCATTTATAGCTCCCC CTCCTCCCTGATGGGTGCAATAATTGTTACAAAAAAACAAGTCTGCATTGTCAATGGCTGTGAAAGAAGT CATAGACTCAACAAAAGCTCCACTAGAGTTTGTAGTGCTTAAACCTCTCGAAAACCATTGATCCTGCGTA CAACTTGAAATAGTAAAGTTCCCTCTATAATTAGAAACATTTGCGTAAGTGATGTATTGAGCAGCAATGG AGCTAGGCGGAGTAACTATGAATTCAATCTCTCTACCACCAAAAGTCAAGTTCGGAAGTTTGAGTGTATC GGTTTCACTGGCCGAAATACAAGATAAAGGAATGAAAACAAGTGAAAGAGGGAGAATTCTGCGAGTCA >gi|15834882|ref|NP_296641.1| polymorphic membrane protein E/F family protein [Chlamydia muridarum Nigg] MTRRILPLSLVFIPLSCISASETDTLKLPNLTFGGREIEFIVTPPSSIAAQYITYANVSNYRGNFTISSC TQDQWFSRGLSTTNSSGAFVESMTSFTAIDNADLFFCNNYCTHQGGGGAINATGLISFKNNQNILFYNNT TIGTQFTGVALRTERNRGGALYGSSIELINNHSLNFINNTSGDMGGAVSTIQNLVIKNTSGIVAFENNHT TDHIPNTFATILARGGAVGCQGACEISHNTGPVVFNSNYGGYGGAISTGGQCIFRDNKDKLIFINNSALG WHNTSAQGNGAVISAGGEFGLLNNKGPIYFENNNASYIAGAISCNNLNFQENGPIYFLNNSALYGGAFHL FASPAANYIHTGSGDIIFNNNTELSTTGMSAGLRKLFYIPGTTNNNPITLSLGAKKDTRIYFYDLFQWGG LKKANTPPENSPHTVTINPSDEFSGAVVFSYKNISSDLQAHMIASKTHNQIKDSPTTLKFGTMSIENGAE FEFFNGPLTQESTSLLALGQDSILTVGKDASLTITHLGIILPGLLNDQGTTAPRIRVNPQDMTQNTNSNQ APVSTENVATQKIFFSGLVSLVDENYESVYDSCDLSRGKANQPILHIETTNDAQLSNDWKNTLNTSLYSL PHYGYQGLWTSNWMTTTRTVSLTNSTETQTANNSIQEQKNTSETFDSNSTTTAKIPSIRASTGGTTPLAT TDVTVTRHSLVVSWTPIGYIADPARRGDLIANNLVSSGRNTTLYLRSLLPDDSWFALQGSAATLFTKQQK RLDYHGYSSASKGYAISSQASGAHGHKFLFSFSQSSDTMKEKRTNNKISSRYYLSALCFEQPMFDRIALI GAAAYNYGTHKTYNFYGTKKFSKGNFHSTTLGGSLRCELRDSMPFQSIMLTPFIQALISRTEPASIQEQG DLARLFSLKQPHTAVVSPIGIKGVYSSNKWPTVSCEMEVAYQPTLYWKRPILNTVLIKNNGSWETTNTPL AKHSFYGRGSSSLKFSYLKLFANYQAQVATSTVSHYMNAGGALVF Protective antigen Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. In addition, pmpE/F-2 was able to engender protective immunity against challenge with C. muridarum [Ref1062:Yu et al., 2009]. PmpG-1 Chlamydia muridarum Nigg VO_0010891 1246433 15834883 TC0263 AE002160 NP_296642 243161 311525 314488 + polymorphic membrane protein G family protein 5.8 98416.45 987 similar to GP:4376736; identified by sequence similarity; putative >gi|29337300:311525-314488 Chlamydia muridarum Nigg, complete genome TGTGATGCAAACGCCTTTTCATAAGTTCTTTCTTCTAGCAATGCTATCTTACTCTTTATTGCAAGGAGGG CATGCGGCAGATATTTCCATGCCTCCGGGAATTTATGATGGGACAACATTGACGGCGCCATTTCCCTACA CTGTGATCGGAGATCCCAGAGGGACAAAGGTTACTTCATCGGGATCGCTAGAGTTGAAAAACCTGGACAA TTCCATTGCGACTTTACCTCTAAGTTGTTTTGGTAATTTGTTGGGGAATTTCACTATTGCAGGAAGAGGG CATTCGTTAGTATTTGAGAATATACGAACATCTACAAATGGGGCGGCATTGAGTAATCATGCTCCTTCTG GACTGTTTGTAATTGAAGCTTTTGATGAACTCTCTCTTTTGAATTGTAATTCATTGGTATCTGTAGTTCC TCAAACAGGGGGTACGACTACTTCTGTTCCTTCTAATGGGACGATCTATTCTAGAACAGATCTTGTTCTA AGAGATATCAAGAAGGTTTCTTTCTATAGTAACTTAGTTTCTGGAGATGGGGGAGCTATAGATGCACAAA GTTTAATGGTTAACGGAATTGAAAAACTTTGTACCTTCCAAGAAAATGTAGCGCAGTCCGATGGGGGAGC GTGTCAGGTAACAAAGACCTTCTCTGCTGTGGGCAATAAGGTTCCTTTGTCTTTTTTAGGCAATGTTGCT GGTAATAAGGGGGGAGGAGTTGCTGCTGTCAAAGATGGTCAGGGGGCAGGAGGGGCGACTGATCTATCGG TTAATTTTGCCAATAATACTGCTGTAGAATTTGAGGGAAATAGTGCTCGAATAGGTGGAGGGATCTACTC GGACGGAAATATTTCCTTTTTAGGGAATGCAAAGACAGTTTTCCTAAGTAACGTAGCTTCGCCTATTTAT GTTGACCCTGCTGCTGCAGGAGGACAGCCCCCTGCAGATAAAGATAACTATGGAGATGGAGGAGCCATCT TCTGCAAAAATGATACTAACATAGGTGAAGTCTCTTTCAAAGACGAGGGTGTTGTTTTCTTTAGTAAAAA TATTGCCGCAGGAAAGGGGGGCGCTATTTATGCTAAGAAACTGACAATTTCTGACTGTGGTCCGGTCCAG TTTCTTGGTAATGTCGCGAATGACGGGGGCGCTATTTATCTAGTAGATCAGGGGGAACTTAGTCTATCTG CTGATCGCGGAGATATTATTTTTGATGGAAATTTAAAGAGAATGGCTACGCAAGGCGCTGCCACCGTCCA TGATGTAATGGTTGCATCGAATGCTATCTCTATGGCTACAGGGGGGCAAATCACAACATTAAGGGCTAAG GAAGGTCGCCGAATTCTTTTTAATGACCCTATTGAAATGGCGAATGGACAACCTGTAATACAAACTCTTA CAGTAAACGAGGGCGAAGGATATACGGGGGACATTGTTTTTGCTAAAGGTGATAATGTTTTGTACTCAAG TATTGAGCTGAGTCAGGGAAGAATTATTCTCCGAGAGCAAACAAAATTATTGGTTAACTCCCTGACTCAG ACTGGAGGGAGTGTACATATGGAAGGGGGGAGTACACTAGACTTTGCAGTAACAACGCCACCAGCTGCTA ATTCGATGGCTCTTACTAATGTACACTTCTCCTTAGCTTCTTTACTAAAAAATAATGGGGTTACAAATCC TCCAACGAATCCTCCAGTACAGGTTTCTAGTCCAGCTGTAATTGGTAATACAGCTGCTGGTACTGTTACG ATTTCTGGTCCGATCTTTTTTGAAGATTTAGATGAAACTGCTTACGATAATAATCAGTGGTTAGGTGCGG ATCAAACTATTGATGTGCTGCAGTTGCATTTAGGAGCGAATCCTCCGGCTAACGCTCCAACTGATTTGAC TTTAGGGAACGAAAGTTCTAAATATGGGTATCAAGGAAGTTGGACACTTCAATGGGAACCAGATCCTGCG AATCCTCCACAGAACAATAGCTACATGTTGAAGGCAAGCTGGACTAAAACAGGTTATAATCCTGGTCCGG AGCGCGTAGCTTCTCTGGTCTCTAATAGTCTTTGGGGATCCATTTTAGATGTGCGTTCCGCGCATTCTGC GATTCAAGCAAGTATAGATGGACGAGCTTATTGTCGGGGTATTTGGATTTCTGGGATTTCGAACTTTTTC TATCATGATCAGGATGCTTTAGGACAGGGGTATCGTCATATTAGTGGGGGATATTCGATAGGAGCAAACT CTTATTTCGGGTCTTCTATGTTTGGACTTGCTTTTACTGAAACTTTTGGTAGGTCCAAAGATTATGTGGT CTGTCGATCTAACGATCACACTTGTGTAGGCTCTGTTTACTTATCCACTAGACAAGCGTTATGCGGATCC TGTTTATTTGGAGATGCTTTTGTTCGGGCGAGTTACGGATTTGGAAATCAGCATATGAAGACCTCTTATA CATTTGCTGAAGAGAGTAATGTGCGTTGGGATAATAACTGTGTAGTGGGAGAAGTTGGAGCTGGGCTCCC TATCATGCTCGCTGCATCTAAGCTTTATCTAAATGAGTTGCGTCCCTTCGTGCAAGCAGAGTTTGCTTAT GCAGAGCATGAATCTTTTACAGAGAGAGGGGATCAGGCTAGGGAGTTTAAGAGTGGGCATCTTATGAATC TATCTATTCCAGTTGGGGTGAAGTTTGATCGATGCTCTAGTAAACATCCTAACAAGTATAGTTTTATGGG AGCTTATATCTGTGATGCTTACCGGTCCATTTCTGGAACGGAGACAACACTCCTGTCTCATAAAGAGACT TGGACAACAGATGCTTTCCATTTAGCAAGGCATGGAGTTATGGTCAGAGGATCTATGTATGCTTCTTTAA CAGGTAATATAGAAGTCTATGGCCATGGAAAATATGAATACAGGGATGCCTCTCGAGGGTATGGTTTAAG TATTGGAAGTAAAATCCGATTCTA >gi|15834883|ref|NP_296642.1| polymorphic membrane protein G family protein [Chlamydia muridarum Nigg] MMQTPFHKFFLLAMLSYSLLQGGHAADISMPPGIYDGTTLTAPFPYTVIGDPRGTKVTSSGSLELKNLDN SIATLPLSCFGNLLGNFTIAGRGHSLVFENIRTSTNGAALSNHAPSGLFVIEAFDELSLLNCNSLVSVVP QTGGTTTSVPSNGTIYSRTDLVLRDIKKVSFYSNLVSGDGGAIDAQSLMVNGIEKLCTFQENVAQSDGGA CQVTKTFSAVGNKVPLSFLGNVAGNKGGGVAAVKDGQGAGGATDLSVNFANNTAVEFEGNSARIGGGIYS DGNISFLGNAKTVFLSNVASPIYVDPAAAGGQPPADKDNYGDGGAIFCKNDTNIGEVSFKDEGVVFFSKN IAAGKGGAIYAKKLTISDCGPVQFLGNVANDGGAIYLVDQGELSLSADRGDIIFDGNLKRMATQGAATVH DVMVASNAISMATGGQITTLRAKEGRRILFNDPIEMANGQPVIQTLTVNEGEGYTGDIVFAKGDNVLYSS IELSQGRIILREQTKLLVNSLTQTGGSVHMEGGSTLDFAVTTPPAANSMALTNVHFSLASLLKNNGVTNP PTNPPVQVSSPAVIGNTAAGTVTISGPIFFEDLDETAYDNNQWLGADQTIDVLQLHLGANPPANAPTDLT LGNESSKYGYQGSWTLQWEPDPANPPQNNSYMLKASWTKTGYNPGPERVASLVSNSLWGSILDVRSAHSA IQASIDGRAYCRGIWISGISNFFYHDQDALGQGYRHISGGYSIGANSYFGSSMFGLAFTETFGRSKDYVV CRSNDHTCVGSVYLSTRQALCGSCLFGDAFVRASYGFGNQHMKTSYTFAEESNVRWDNNCVVGEVGAGLP IMLAASKLYLNELRPFVQAEFAYAEHESFTERGDQAREFKSGHLMNLSIPVGVKFDRCSSKHPNKYSFMG AYICDAYRSISGTETTLLSHKETWTTDAFHLARHGVMVRGSMYASLTGNIEVYGHGKYEYRDASRGYGLS IGSKIRF Protective antigen Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. Overall, vaccination with DCs transfected with PmpG-1(25-500) exhibited the greatest degree of protective immunity among the four Chlamydia Ags tested [Ref1062:Yu et al., 2009]. RplF Chlamydia muridarum Nigg VO_0010892 1246168 15835415 TC0801 AE002160 NP_297174 243161 941594 942145 - 50S ribosomal protein L6 10.64 18060.75 183 ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance >gi|29337300:941594-942145 Chlamydia muridarum Nigg, complete genome TCTATTTTTTACCAGTTTTCGCTGCTTTTCCAGCCTTACGACGTACATATTCGTTTTCGTAACGAATTCC TTTACCTTTGTATGGCTCTGGAGGGCGTTTTGCACGAACACAAGCCGCAAATTCTCCTACCAACTGCTTG TTGATACCTTTGATGGAGATCAATGTGTTCTTCTCAACAGAGACTTCCAATCCTGTAGGAATAGGCATTT TTGTTGGATGGGAAACCCCTATCGATAAATCTAAGAAGGACCCCTGTACCGAAGCTCTGAATCCAACTCC GATCATTTCTAAACGTTTTTCAAATCCAAGGTGGACACCTTTGACCATATTTGCTATCAAAGCCCAATAA AGCCCTTGCATACGACCAGGTCTGTCTATAATGTGAGCTGCGGGAGAAACAAACACCTCATTACCTTTAA CGGCAATCTCAACTTCTTTAGCCAACACCTGTGTCAAAGACCCTTTAGGACCTTTTACTGAGATTTCATC ATTTTGAATAGAGACCTCTACGCCTTGAGGAAGTACAATAGGGTCTCGAGCTTTACGAGACA >gi|15835415|ref|NP_297174.1| 50S ribosomal protein L6 [Chlamydia muridarum Nigg] MSRKARDPIVLPQGVEVSIQNDEISVKGPKGSLTQVLAKEVEIAVKGNEVFVSPAAHIIDRPGRMQGLYW ALIANMVKGVHLGFEKRLEMIGVGFRASVQGSFLDLSIGVSHPTKMPIPTGLEVSVEKNTLISIKGINKQ LVGEFAACVRAKRPPEPYKGKGIRYENEYVRRKAGKAAKTGKK Protective antigen Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. In addition, RplF was able to engender protective immunity against challenge with C. muridarum [Ref1062:Yu et al., 2009]. TC0439 adherence factor Chlamydia muridarum Nigg VO_0010887 1245792 15835057 TC0439 AE002160 NP_296816 243161 526698 536375 + adherence factor 7.3 344966.97 3225 similar to GP:6013469; identified by sequence similarity; putative >gi|29337300:526698-536375 Chlamydia muridarum Nigg, complete genome CTTGGAACAAGCATTGTGTACTATGACTCTTTCGAATACAGCCTCTTCCACAGTTTCACCTAAGTCTACG CAACTTAACCATACTTTATCCAATCAAACTCACCCTGTTGCTCAAAGCAATAGGAATATAGATTTCCAAA TCTATGATGAACAGATTCATAATAATCATTCTACAGAAGATGTAGTTGCTATAGGACGTCGTATTCAGCA AGAATATGCGAATCTTACCACATCAAAACAGGTTAATTTCTCGGCATCTCCATCTAACCATACTGGAAAC TGGAAGATTTCTTTGCTTTATAATTTATCGATGCTGGTTGCAAATCTATTCCCTACAACTATTCAGCCTG TGCAACCTCAAAAAGTCATCTTTAAGAATCAAGACTCTTCTAAGGAAACCCAACTGACCAAATCATCCAA AGTAGTTTCTTCTCGCTCTTCTTTAAAAGAGATTTTTTCGAAGAAATCTCCTCCTTTGAGAAAGTCTTTT TCTTCCTCTAAAATTTCTAGAGCAATCCCTAGAAGAAAAAAACGATCAAACGATGATCCAGTTCAAGCCA CAAATACTTATGATTTGACCGCTGAAAATATTCTTGAAAAACTCTCTTTAACACAAGAACAACAAATTAA GCACGATAATCTGATTAGCAATCTCAAAGAAGCAATTAATCGATATAGTGATCTTAATCGGAAAAATTCC CGGAAAGGACAATCCCTTCTTGTTAGGCAAGCCAAAATTCTAGATGAAATTCTTTCACAGACTAAATCTA CAGAGGAACGAGCTTCCAATTCTGTAATGACGACTATTAAGAAGGAATTTACCTCCCACCGCGTACCTGT AGAGAAAAATATTCACGGAATTTGGATTGCAGGTTCACCGCCAGAAGGGACCGATGAATATATTAAATTG TTCCTTCACACTTATCCAGAGTTTTCCTTCCTTTTTTGGGTGGATAAAACAGCTTATGGCGCAGCAAAAT TCTCTTCTACTCTTAAACGAATTGCTTTTGATGCAGCAGTTAATTCCTTACGAGAGGCTACTCCAGAGCC TGTCAAGCAGTTTGTTCAACGTTATGATAAGCTAAAAAAATCATACGATACTTCGCGAGATTTTGATGAA AAACAGCGGTTATCTGAGCAGTTAGTTGAGCTATACGATAATTACAATAAATTTAGTAAGGAGATTCAAA GTAACTTTGATGTTTTGCTATTGCATGAAATGATCACTATTCAAGATAGCTTTTTTAACTACTGCCAGCT CAAAGGAGTAGGTGCAATAACAGATGAGACACGTATAGAGTATCTTGAGAAAGTTTTGAAAGTAAAAGAA GAGGATCTATCTCACTATAAAGAAACTATCAAACGCAACAAAGAGTCTATTGAAAAACTTGTAAAAGAGA TTAATGACTCTACTGGACGGGAGAGAGTAGTTATCAAAGACATCCGAGATTTGAAGTCTTTACAAGATCT TACTAATAGCTACAACTATGAAACAGAAATGTTACTCCGATGGAATTATGCAGCGGCTACCGACCAGCTT CGTATGTACATGCTTAAAGAATATGGGGGGATTTATACAGACCTAGATATTATGCCACAATACTCTCAGG ATGTTTTGCAAAAAATTATGGATGTTGGTGGGAGTAGGTTTTTTGAGCATGATAAGTTACGGCGGACTCT TTCTTTTGCAGCTCTAAAATTAGGAAGCGGTAAGCAAACAACTGTTTCCTTTGAAGAGGCTAAAAAAGCT ATGACTCTCCCCACATTTACTCTTCAGGATAAGAGTCAAATTTCTGAAATTTTTAAATATCTAGAAACGG AAACGCAAGCAAAAAAATCACTTTTCCAACCGATGGATGTTACCGTCGTCCGTGATTTCATGCCTATTTT ACAACGTTATCATAAGTGGCAAACAGGATGGAATGTTCGGGGATTAAATGGTTTAATGATGGCTCATAAA GATAGTGCTGTTGTCGATGCTGTTATTGCTCGCCAGCGAGCGGCCTATGATGAGATGCGTGCACTTCGTC AGAATGTTGTTAGTGGAGAATTTTTTCGTTCTCTTGGAGATCTTGAACATGTAAATCGTGAAAAGAATAT CGGCGGCTATTTAGCGAAAAATTATCTTGGGGGCAGTCTTTTTTTCGATTTTCGTCAAGATTCAGTGATT CCAGGAGCTATTAGTACCTTAGGAATTTCAGGTCCCGATATCATCATGGATACCATGAGCGATTATTTTA CAAATCTTGGACCAGTTGGAGAAGATTTTTTATATGAAGGGAAATTAGGAAAAGCAGCTTTCCTTGGAGC TTATCAAGCACAAAAAACTCCGAAAGGAGAACTTACCTATGATTGGCTCCATCCTTTATCCATTGGAGCT AATGACGTTACTCCTGCTGATGCAAGCACCTGGTGTGAAACTCGACAGCATTGTGCCGCTGAGCTTCTTT TATCGGACTCTATATCCTCAGACGAACACCCTAAGGGTATTCGTCGAGAAAGAGTAAACCCGAATGATTT CTCTAAATTATGGAGTAAGGAAGCTCAGGGAATCCTTTCTAGTGATTTCGCGGATCTTTTACCACGCTTT AACTTACTGATAGAATCATCTGCGTTAGATATTCATACTTTATCGGCTCTTGATCGAGATATCCAACACC TTTTTACAAAAGTTCAAAAAGACCCTGTAGCATCTGTAGCTGTATTTTCTTTACAGTTACAATTAGCTGA AATGATTCGTGCGATTCCTTTCCCTATACGTAATCAGGTACATATCTTGCCTGAAGCACAAGCACATTTC GAAGCAGATTGGAAGAAAGCAATCCAATTATATCTTCACAGTCATCCCCAAACAGAAGTGGTTATTTGGT ACAGTTCTACTCATACTCAAATTGTTTTTGGAAAAGATTTGCTTGCTGTTGCAGAACGAGTTGCAGCAGC AAAATCCTTAATGTCTGATCATGATCCATCACTTATAACCAGTTATCTAAAATATAAAACTCAATCTCAT CTTGGGGTGCTTACTGAGTTTGATCAAGAAGATTTCTTTGAACTGATGGTAGATATTGCAGAGGAACCAG AGCTTCATAAACAACTATTGAAAATAGAAGAACAAGTTAATTCAGGACTCTACTCTCATGTAGAGCATTC TTTAGGAGAGTGGTTAAAATTATCCAAAGAAGAAAGAAAATCAAAGTTTCTTAAGATTTTAAAAGAAACG TTTCAAGAGGAGGAAAGAGAAGACTCTCAACAACAACATAAAACATGGTTTGAAGAACTCTATGAGAAAA GACATCAGGAGCGCGTAAAGGATCCAGCAAAAAAAATCCAAGAACTAATCACAGTTTTTCAAGAAAGTCA GCGCGTTCAAGCTCAAGACATAGATACTTATTTTGCTCATAAACCGTTTTATCAAGATCTGATGAAAGAT GGTTACGCTTTTGAGGACATTTCTGTGATTACGAAGTATCTTTTAGCGAGTGACGGTGTTTCAGGGATTA TTACTACTGATCCTATATTTCCTCCTCCATCTAAACAGCTAATTGACGCTATGAAACAATCTTTGGGAGA AGATTTTGGTGAGCTACACTATACGTTACAGATGGTCTATGATTGGTTATCTAAAGAGACTAACAGCGTA ACTTCTGAACAGGCAAAACAAAAATTACCACAGAAACTACATGAAAAACTAGAAGGTTATACAACACATG ATTTGCTTATTCCGCCTATTGATGGAAGCGTCAGCGCTTTAGGATTGCGATTTTCCACGGAGGAGGGGAA GGTATCCGATCGCGTTTTAACAAGCATAGCACCTGGAGTTCCTAATTCTGCTAGCTATGCTATGACTAGT TATTTGTATGGTTTGTTCTTAATTACGAAAGATATCCAAAGTGGTCGATTAACCCATGAAATACTAAAAG AGAGATTACAAACCTATGGAGGCGCCTATTTTATCAATGAATCAAAAATTGATGTGCTACTTGCTTTATC CCGGAAAAAAGCTCAGATATCCCTAATAGACGCCCATAAGGCTCTTACAGGTTTTTCTTCTTTTAGCGAA GCATCTCTGGCTTTACTTACAGGAAGGATGCCCGGAACAAGCAGAGTTCTATCAAGAGAAGTAGAGTTTG GACGACCTTCAGCTATCGTTATGGAAGGAGCAACAGCTATTCGTGCTCAGAGTTACGATGCTGTTGGATT AAGGAAAGATTTTTTCTTGTTACCTCATACAGTTCCTTCCATACAGTCTATTGTTGAGCAAGCGAAATAT ACAGTATTATCCTGGCCAGAATTTTATGAAAATCATGCGGATAAATGGAACGATCTTGCTAATCGTTTTG GAGCTGAGGACCTTAGTGTTCATCCGCAAACATTTTTATATGACACAGAAGGTCGCTGTATGGGGCTTGC TTTACTGTATATGTTAGCAGATGACTCTGTATCCTACAGATTATTACAACAAAATCTGATGACTTTAGCT TCGTTATTTGATGAGCAAAACAGAAGAAATATCCCTTTAACTCCAGCTGATCAGAAATTTTTAAATAAGG GGCTTTCTTTAATTGAGTGGTTACAGTTCAAAGGAAATCAACAACTTCAAACAGAGGGATTTTTCCATAC CTTAGACTGGGATATCCCTCAATTAATGAAGCACTTTGCTTCTTCAACTGTAAAAAGTTGGTTAATTACT ACGCCTGCGCATAGTCTTGTTCTTTCTTTAATGGGGAATTTTTTTAGAGTAACAGATCCAAACTATGGCC ATACAGACTTTCCTTCATTAGAAGCTGCTATTACTTTTTTAGAAAGGATGGTTCAAGTATCCCCAGCTGT TTTGGAGCGTTATGGATTTGATAAGGAAAAGTCTGTAACTAGCCAACTCAAAGTCCACTCTTTAGAGACT TCAGAATTGCAAAATGCAGTTTTTGCTTCCTCAGATCTTGGCTTTACTAGTCGCTATTTTACGACAACGT TAGAAGAGATGACCGTCAGAGGCCCTATTACGATGAATCAACGTCACACGGATTGGGCCACTCTTTATAA GATTGGGGGGACTGTTCAAGGTAAACGTATTGATAGTAGAACACGGGAATCGGATTTAAATTTTTTAAAA ATTAATGGGGATATTCTAGAGGAATTTTTAACAAGAACTGTTCTAGATAGTGACTTGGTGGAGTTAATAC AATCTCTGCTCAAAACACATGGCCTTGAGCCAGGCACTACCCTTATTAGTCCAAGTTCTATTGTAGAGAC CGCAATAGACCACGTCTCGCTATTACAAGCGGTGAAAACAAAAACCTCTCGAATGCACACTATTTTACAA AGCCTAGGAGAAAGAATTTTTAAATTGTTTAAAAATTCTGGGGTACAAGACTCAGACAAAATTTCTATAG ACAGAGTCCAACTAGTTGATGAATCTGATAGCGCAACGATAGACTTCACAGTGATTAAAGATAAACAGCG TTCTCAGAAAAAAAGTATTACTGTTGGCATAGAAAGTTTAGCTGGATCCTTTAGAAAATTTAGCGCATCG ATGCACGAAGTTATTGGAACTGGAGTGCTTGATTTAGACTTAGGAATGACTGTCGTCTCTTTAGTTCAGT ATGTCCGTTTAGTAGAAGCAGGACAAGGAAAAGATGCTTTAGCAGTTGCTAATTTAGTGATGAATCTTAA AATAGCTCTCGAGGTCTCTATAGGGAATGTAATTCAGGCTCTGGGTAAAAAACTGCTCACTCAGGAGGGG CTTAATACTTTCCGTTTAGAAACAGAATTAGCTCGTCAACTACACAAAGTGGGAGCACGAGTTGGAGGAA CTGTTGGTAAGACGTTAACCAGAGTAGCGCATGTATTAGAACTCCCCATATTAGAAAGTGCAATTGGAAC ATGGAACCTCTATAATAGTGTGAACGAGTTATTACATGCTGATTCTTGGAGTGACCAGGTTGCTGCTAGA GTACAAGTAGCTTTTGATTCAATCTCTTTAGGAATCACTATAGCTTCGGTTGTTTCTCCAGCACTTATGA TGGCAGCAGGCCCCATTGCTGCCATAGGGATGGGGGCAGCATCCATTGCTCGTAATGTTGCCAGAAAAGA AGAGCGGCACTCTCAATGGCTCAAGTACAAGAGTTTTCTTGATAATGGATCAAAACATACAGTTGCCGCA TTCCCTCATAAAGGGCTTATAGATTTATCTGAGAATTTAGTACTTGGGAACATTGTGCTGAATTTAAGGG TTTACCCACCTCTGCTTACAGGCGATCGCTCTTATAATGCAAATCGGTGGTTTGGGAATAAGCCTGGATG GTCTGATTGGCAAGTTCGAGAAAGATTAGGTTATGCCTATCGAAGCAGTCCATCTTATGCGTTAGCCAGA GGACACGCTAATAGTTTTTGGCCACTATCTATGCCTAGTATTGATAAAGGTGTATATCGCACAGTAATTC TAGGATATGGCATTCAATATAAAGCGGTTACAGAAGTTGTCTACTTGTCTAATGAAATGGTTTGGAGAGA AGCTGTGATGCAGTTTGACTCTCGATACTATGTAGAGCCATTAACAGCGGAAAAGGAGTGTGCAACAGTA CTAGCAGGAGATACGCCACTATCAGTGATTCCTGTACGTTTATTAGAAGAGGAGTCTCTGGAACGCGAGA AGAACGCGGCTTCTTATAAGAACTATCAGATCATTATTGAGGGAGGGAAAGGAGGACTTACTGTACAGAT AGGAGGAGCAGGTTATTACAAGTTAACAGCACAGCCAGGAAAAGGGAATATCCTCTCTTTCCGAGCAATA CCTGGATATCTTTCTGTAACGTTTGATCTTAGCCGATTGGAACAAGAGGTGCCTCTCACCAAACAAAATG GAACAGCCTTAAAGATACTTAAAGTACGTCAAACTGGGTTTGATACTATTGTTGGATCTTCAGGAGGTAG TGATAATTTAACAGGCAATCACAATACAAAATTTTATCTAAGCACCGGAGGAGGACATGTCACCTCAGGA TCCGGTAAGAACTGGTATAACATTCCATCTTTAACACGTAGCCTTGGTTTTGATTTTACTTCTAATGCTA TAGAGCATAAGGCTTCTGTAGAGATGCTTTTAGCAGATTTCTCTCCTGCTAATAATTTAAGTTTAATAGT TGAACGATTTGCAAATGTTAGCATTCATGTTTTTAATTATAGCAATAAAACAGCCCCTTATACTGTGAAT TTGAAAGACGGGGTGTCTTTACAAGCTTCTAAGCAAGGCTTTAGCGGTAGTTTATTAGAGGTGGCTTCCT TTGATCAAACGCTATGGCAAAAGAGCTATCCAGAAGATAGAGGATTTGTAGAAGATATTTTATCTTGGTT ATTAAAACTGCAATGGTCGTTAGCTTCTCGAGTAAGGATCTTTTTACAGGGAGGCACAGCACAATACGAA ACAACGCAGAAAAGTCTTATTTATAGACCTGATCCACACTCAAGTATATCTATTCAGGCTACAGATTCTT ATAATACACAAGTTTATGGTAACGTTGGCTGTTCTTATATTCTATTCTCTTCACCAGGTGTGACCGCCAA AACACTCGATATTTTGTTATTCGAAGATTCGGGGTTACCACAAATTATTGACTTAAGCACACTTGTTCCA ACTTCTATCCAAGGGTTCCTCTATCCAGGAGGGTTTATAAACTTTCAAGTCTCTTCTGCAAGATATGCAT TTCCTTTTTCTGTCAGTTGGAGAAGGCATCATTACACTTTCCCTGCTCAAACGATTATTCAAGTTCTACC TCGCTTGCGGTTTGAACTAGGGGATTGGTTTACAATTTTGCAAAAGAGTGTAGGAAAATGGGTAACATTG TACCAACACGACATGATTATCCCTGAACGTATTGAAGGTGTTTTAAGTCTAAATAACACAGCGACATTAA TGTCACACCACAATCAAACAACCCATATGCTTGGAGTAGAAAACAGGGGAGATTTGAGTCTAAAAGTTTT AGGAATACTCCAATCTGGTCAAATTAAAGGATCTAAATCGGAAAATAGCGCGAGTGGTCATTTTTCTAAA TTGTTATCTGTCCCAGCACATACGATCAAAAATCTTTCGTTTAAAGAGGAAGAGGGAACTAAGAGTAAAA ATATTTTATTCTACAGTGTGCTGGAGGAAACATTCCTTAAAGCTACAGATAAACCTTTAACTATTATTAA ACGTGATAAATGGTCTCTGTATGATGAAATCCAAGTATTTGCAACAACTCTTAATCTGCAAAATTTCTTG CGTTATCATATTTCGGAAGAGACTCCAATGTTATCTAGACTGCTCATGTATGCGCAGAAACGCGTTTCCA TCCAAAATAGAGATCTCGCATTGAAATTTTTCTATGTTCGAGAGCGAAGTGGTATTGGTGCAATTAGATT GGTATTCAAGAACTTTTTCGAAGAGAGCTTGCAGGGGATATTACATGGAACCTTAGAGCGAGAAGTTAAA CCTATGCTAGCTGAAAATCCAAATACACTAATTCATTCATCGTATCGGAATCATTTAGAACTCATCCTAG GGGAGGAAACCTTAGATCTCGCAATTATCGTTCAAGAGTTTAGCTCTTCAAAAAATATTGTTGAGTTGCA AAAAGATCTAGCAACCCATCGGCTTATTTATCCTCAAGGAAAAGAAGCCTTGTCTCTTGCAATCTATACC CATACTTTCAGTAAAGAGAGCTTAGTCACATCTAAACAACGAAGCGAAGGGTTAAAATTCATCGATCCAT CAATGCAGGAATATCGTTTACCTGAGACAACGATTCTCGAAAACTCTTATTATTTAGACCCTTCCACAGG AGATTTGTATTTAACAAAGATAATTGCGCAACCTTCACAAAGTAGAGCATTTCTTATCAAATTTAAAAAA TACAAACGCCACTGGCTAGATTTCCAAAAATTAGTTCTTTCTGGATCTCATTTAGAACTTATCGCATCTA CTGGAACAGCTTTAACATTTGTAGGCCCCGAATTACGGCATTTAGAGATTGACTTCCCAGGCGCGTTAAA TGGGACTATTGCCAAAGAACACATCTCATCTCGGGCAAGTATTGTAATCCCAACAAACAATCAGGTGACA CACTATGACCCAAGAATAGCGAAGCAGCATTATAGCTCTATCAATTATATGCTGTGGGATCTGCGTGATC GTGCGACACTATCTAAGCGAGCAAAAACTTTAGATAGCTATTTGTTAGAAGTTTGTATGAACTTAGACTC TTCTAATCCAACGTGGAATATTCCAAATGATGTGTTGAACTACGCGGTAGGATATTATCGAACAATCCTT CCTAGCTGGGTAAAAAATAAAATCCGTGTAGGTTCCTTAATTAAAGTAGAAGCTGATACTGCAAAAACTT TGAGTTTCTCACTAATTACGACACAAAATGATCTATTCCAACCTGTAGTGGATAAAGGATTCTATATTTA CTACAGCGTTTCGAAACTAGCCAATCATGTTAAACTGCGAAATGTTACTGGAGATATGTTGCTTGATATC GATAAGGAAACCACATTTATTGTTCGAGGAGTAGATGAATCTGATTATGATAAGAAACAAATCTATGTTG TTTTAGATCTGACAACAGAAGAAGAACGTAAATTGCGAACGGATAAGAACATCATTATTATTCCTGGAGG AGAACGATTGAACCGATG >gi|15835057|ref|NP_296816.1| adherence factor [Chlamydia muridarum Nigg] MEQALCTMTLSNTASSTVSPKSTQLNHTLSNQTHPVAQSNRNIDFQIYDEQIHNNHSTEDVVAIGRRIQQ EYANLTTSKQVNFSASPSNHTGNWKISLLYNLSMLVANLFPTTIQPVQPQKVIFKNQDSSKETQLTKSSK VVSSRSSLKEIFSKKSPPLRKSFSSSKISRAIPRRKKRSNDDPVQATNTYDLTAENILEKLSLTQEQQIK HDNLISNLKEAINRYSDLNRKNSRKGQSLLVRQAKILDEILSQTKSTEERASNSVMTTIKKEFTSHRVPV EKNIHGIWIAGSPPEGTDEYIKLFLHTYPEFSFLFWVDKTAYGAAKFSSTLKRIAFDAAVNSLREATPEP VKQFVQRYDKLKKSYDTSRDFDEKQRLSEQLVELYDNYNKFSKEIQSNFDVLLLHEMITIQDSFFNYCQL KGVGAITDETRIEYLEKVLKVKEEDLSHYKETIKRNKESIEKLVKEINDSTGRERVVIKDIRDLKSLQDL TNSYNYETEMLLRWNYAAATDQLRMYMLKEYGGIYTDLDIMPQYSQDVLQKIMDVGGSRFFEHDKLRRTL SFAALKLGSGKQTTVSFEEAKKAMTLPTFTLQDKSQISEIFKYLETETQAKKSLFQPMDVTVVRDFMPIL QRYHKWQTGWNVRGLNGLMMAHKDSAVVDAVIARQRAAYDEMRALRQNVVSGEFFRSLGDLEHVNREKNI GGYLAKNYLGGSLFFDFRQDSVIPGAISTLGISGPDIIMDTMSDYFTNLGPVGEDFLYEGKLGKAAFLGA YQAQKTPKGELTYDWLHPLSIGANDVTPADASTWCETRQHCAAELLLSDSISSDEHPKGIRRERVNPNDF SKLWSKEAQGILSSDFADLLPRFNLLIESSALDIHTLSALDRDIQHLFTKVQKDPVASVAVFSLQLQLAE MIRAIPFPIRNQVHILPEAQAHFEADWKKAIQLYLHSHPQTEVVIWYSSTHTQIVFGKDLLAVAERVAAA KSLMSDHDPSLITSYLKYKTQSHLGVLTEFDQEDFFELMVDIAEEPELHKQLLKIEEQVNSGLYSHVEHS LGEWLKLSKEERKSKFLKILKETFQEEEREDSQQQHKTWFEELYEKRHQERVKDPAKKIQELITVFQESQ RVQAQDIDTYFAHKPFYQDLMKDGYAFEDISVITKYLLASDGVSGIITTDPIFPPPSKQLIDAMKQSLGE DFGELHYTLQMVYDWLSKETNSVTSEQAKQKLPQKLHEKLEGYTTHDLLIPPIDGSVSALGLRFSTEEGK VSDRVLTSIAPGVPNSASYAMTSYLYGLFLITKDIQSGRLTHEILKERLQTYGGAYFINESKIDVLLALS RKKAQISLIDAHKALTGFSSFSEASLALLTGRMPGTSRVLSREVEFGRPSAIVMEGATAIRAQSYDAVGL RKDFFLLPHTVPSIQSIVEQAKYTVLSWPEFYENHADKWNDLANRFGAEDLSVHPQTFLYDTEGRCMGLA LLYMLADDSVSYRLLQQNLMTLASLFDEQNRRNIPLTPADQKFLNKGLSLIEWLQFKGNQQLQTEGFFHT LDWDIPQLMKHFASSTVKSWLITTPAHSLVLSLMGNFFRVTDPNYGHTDFPSLEAAITFLERMVQVSPAV LERYGFDKEKSVTSQLKVHSLETSELQNAVFASSDLGFTSRYFTTTLEEMTVRGPITMNQRHTDWATLYK IGGTVQGKRIDSRTRESDLNFLKINGDILEEFLTRTVLDSDLVELIQSLLKTHGLEPGTTLISPSSIVET AIDHVSLLQAVKTKTSRMHTILQSLGERIFKLFKNSGVQDSDKISIDRVQLVDESDSATIDFTVIKDKQR SQKKSITVGIESLAGSFRKFSASMHEVIGTGVLDLDLGMTVVSLVQYVRLVEAGQGKDALAVANLVMNLK IALEVSIGNVIQALGKKLLTQEGLNTFRLETELARQLHKVGARVGGTVGKTLTRVAHVLELPILESAIGT WNLYNSVNELLHADSWSDQVAARVQVAFDSISLGITIASVVSPALMMAAGPIAAIGMGAASIARNVARKE ERHSQWLKYKSFLDNGSKHTVAAFPHKGLIDLSENLVLGNIVLNLRVYPPLLTGDRSYNANRWFGNKPGW SDWQVRERLGYAYRSSPSYALARGHANSFWPLSMPSIDKGVYRTVILGYGIQYKAVTEVVYLSNEMVWRE AVMQFDSRYYVEPLTAEKECATVLAGDTPLSVIPVRLLEEESLEREKNAASYKNYQIIIEGGKGGLTVQI GGAGYYKLTAQPGKGNILSFRAIPGYLSVTFDLSRLEQEVPLTKQNGTALKILKVRQTGFDTIVGSSGGS DNLTGNHNTKFYLSTGGGHVTSGSGKNWYNIPSLTRSLGFDFTSNAIEHKASVEMLLADFSPANNLSLIV ERFANVSIHVFNYSNKTAPYTVNLKDGVSLQASKQGFSGSLLEVASFDQTLWQKSYPEDRGFVEDILSWL LKLQWSLASRVRIFLQGGTAQYETTQKSLIYRPDPHSSISIQATDSYNTQVYGNVGCSYILFSSPGVTAK TLDILLFEDSGLPQIIDLSTLVPTSIQGFLYPGGFINFQVSSARYAFPFSVSWRRHHYTFPAQTIIQVLP RLRFELGDWFTILQKSVGKWVTLYQHDMIIPERIEGVLSLNNTATLMSHHNQTTHMLGVENRGDLSLKVL GILQSGQIKGSKSENSASGHFSKLLSVPAHTIKNLSFKEEEGTKSKNILFYSVLEETFLKATDKPLTIIK RDKWSLYDEIQVFATTLNLQNFLRYHISEETPMLSRLLMYAQKRVSIQNRDLALKFFYVRERSGIGAIRL VFKNFFEESLQGILHGTLEREVKPMLAENPNTLIHSSYRNHLELILGEETLDLAIIVQEFSSSKNIVELQ KDLATHRLIYPQGKEALSLAIYTHTFSKESLVTSKQRSEGLKFIDPSMQEYRLPETTILENSYYLDPSTG DLYLTKIIAQPSQSRAFLIKFKKYKRHWLDFQKLVLSGSHLELIASTGTALTFVGPELRHLEIDFPGALN GTIAKEHISSRASIVIPTNNQVTHYDPRIAKQHYSSINYMLWDLRDRATLSKRAKTLDSYLLEVCMNLDS SNPTWNIPNDVLNYAVGYYRTILPSWVKNKIRVGSLIKVEADTAKTLSFSLITTQNDLFQPVVDKGFYIY YSVSKLANHVKLRNVTGDMLLDIDKETTFIVRGVDESDYDKKQIYVVLDLTTEEERKLRTDKNIIIIPGG ERLNR Protective antigen Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0439 showed significant levels of protection when compared to negative control animals [Ref1057:McNeilly et al., 2007]. TC0512 outer membrane protein, putative Chlamydia muridarum Nigg VO_0010885 1245872 15835130 TC0512 AE002160 NP_296889 243161 618235 620613 + outer membrane protein, putative 9.51 84655.32 792 similar to GB:X75201, and PID:435005; identified by sequence similarity; putative >gi|29337300:618235-620613 Chlamydia muridarum Nigg, complete genome AATGCTTGGAATACGAAAAAAAACGATTCTGCAACTCGCTATTTTACTGTTGCTCCCCTTTTCACAAAAC GCCTTTGGAACATCTCCTGAAGGACGTATGGTGGTACAATCCATCACTATTGAGACTCGAGGAGAAAATG CTCAAAATAAGCGGGCTATTCCCAAAATAAAGACAAAGCAAGGGACGCTGTTCTCTCAAACTGATTTTGA CGAAGATCTAAGAACTCTCTCGAAAGATTTCGACCGCATTGATCCTATTGTAGAGTTTCGCAATGGGCAA GCTGTTATTTCCCTTATTCTGACAGCAAAACCCGTTATCCGACACATTAACATCTCAGGGAATGAAGCAA TTCCTACTCATAAAATTTTAAAAACCCTAGAGCTTTATAAAAACGATCTTTTTGATCGAGAGGTTTTTTT CAAAAATTTTGATGCTTTAAGAACCCTTTACTTAAAACGCGGATACTACGATTCTCAATTGTCCTATTCT CACGATCATAATGAGCAAGAAGGTTATATTGATATCTCAATCCAAATTAAAGAAGGGCCCTACGGACGGA TAAAAAAATTAACAATTTCAGGTATTACTCGTACAGAAGCCTCTGATTTGGGAGACATAGTTTTAACCAA ACAGTACTCTACAACAACAAGTTGGTTCACTGGAGCAGGGGTATACCATCCAGACATGGTGGACCAGGAT CTCTTTGCCATAACAAACTATTTTCAAAATAAAGGTTATGCAGACGCAAAAGTCACAAAAGAAGTCTCCA CAGATGCTAAAGGAAACCTCTCTCTTGTTATCAACGTAGATAAAGGGCCCCTGTATACTTTAGGTCACGT ACATATAGAAGGTTTCACAGCTTTATCCAAGCGGTTACTTGATAAACAACTGTTAGTGGGCCCCAATTCC TTATATTGCCCAGAAAAAGTTTGGGCTGGAGCTCAAAAAATCCGTAATGCCTACGCTAGATACGGATATG TAAATACCAATGTAGACGTATCTTTTGCTGTTCATCCAACGCTCCCAGTTTATGATGTTACTTACAGGGT TAGCGAAGGAGCTCCTTACAAAATCGGACTAATCAAAATCAAAGGGAACACACACACCAAACATGACGTA ATTTTACATGAGACAAGTCTGTTCCCTGGAGATACTTTTGATAGATTAAAACTAGAAGATACGGAAACTC GATTACGTAACACAGGCTACTTTAAAAGTGTGAGTGTCTACACGGTACGCTCACAATTAGATCCTTTGGA CTCAAATAACCTTTATAGAGACGTTTTCATTGAAGTCAAAGAAACCGAAACAGGTAACCTAGGACTGTTC TTAGGATTCAGTTCTATAGACCATCTGTTCGGAGGAGCAGAAATTTCAGAAAGCAATTTTGATTTATTTG GCGCCCGACACTTATTCAAAAAAGGGTTTAAATCCTTAAGAGGTGGTGGGGAATATCTTTTCTTAAAAGC CAATTTAGGAGACAAAGTTACTGATTATACTGTTAAATGGACAAAACCTCATTTCTTAAACACCCCATGG ATCCTAGGAGTAGAATTAGATAAATCTATTAATAAGGCTTTATCAAAAGATTATTCCGTTGATACCTATG GAGGAAACATCAGCACGACATACATTCTTAACGACAAACTGAAATATGGGATGTATTACCGCGGGAGCCA AACGAGCCTAAGTTTAAGAAAAAAAACAGAAAATCCTAACAAACCTGGACCAGATCTAGATAGTAACAAG GGATTTGTTTCCGCGGCAGGGCTTAACGTTCTTTACGATTCTATTGACAATCCTAGAAAACCTACTATGG GTATTCGGAGCTCTTTGAACTTTGAGTTGTCTGGATTAGGAGGAACCTATCAGTTTACAAAATTAACAGC CAGCGGCTCTATCTATCGCCTGCTAACTAAAAAAGGTGTTTTGAAAATTCGTGGAGAAGCTAAGTTTATT AAACCTTTCGGTACAACCACAGCCCAAGGAATTCCTGTTAGTGAGCGCTTCTTCTTAGGAGGAGAATCTA CCGTTCGTGGATATAAACCATTTATTATTGGGCCTAAATTTTCTCCAACAGAGCCTCAAGGGGGCTTATC TTCCTTATTGCTAACAGAAGAGTTTCAATATCCTCTAATTTCTCAACCCAGCATCAATGCATTCGTTTTT CTAGATTCGGGCTTTATTGGCACGGAAGAGTACACTATTCGACTCAAAAATCTCTGTAGCAGTGCCGGAT TTGGGTTGCGTTTTGATATGATGAATAACGTGCCCATTATGTTAGGTTGGGGTTGGCCTTTCCGCCCAAC AGAAATTCTTAATAATGAAAAAATTGATGTATCTCAAAGATTCTTCTTCGCTTTGGGAGGAGTGTTCTA >gi|15835130|ref|NP_296889.1| outer membrane protein, putative [Chlamydia muridarum Nigg] MLGIRKKTILQLAILLLLPFSQNAFGTSPEGRMVVQSITIETRGENAQNKRAIPKIKTKQGTLFSQTDFD EDLRTLSKDFDRIDPIVEFRNGQAVISLILTAKPVIRHINISGNEAIPTHKILKTLELYKNDLFDREVFF KNFDALRTLYLKRGYYDSQLSYSHDHNEQEGYIDISIQIKEGPYGRIKKLTISGITRTEASDLGDIVLTK QYSTTTSWFTGAGVYHPDMVDQDLFAITNYFQNKGYADAKVTKEVSTDAKGNLSLVINVDKGPLYTLGHV HIEGFTALSKRLLDKQLLVGPNSLYCPEKVWAGAQKIRNAYARYGYVNTNVDVSFAVHPTLPVYDVTYRV SEGAPYKIGLIKIKGNTHTKHDVILHETSLFPGDTFDRLKLEDTETRLRNTGYFKSVSVYTVRSQLDPLD SNNLYRDVFIEVKETETGNLGLFLGFSSIDHLFGGAEISESNFDLFGARHLFKKGFKSLRGGGEYLFLKA NLGDKVTDYTVKWTKPHFLNTPWILGVELDKSINKALSKDYSVDTYGGNISTTYILNDKLKYGMYYRGSQ TSLSLRKKTENPNKPGPDLDSNKGFVSAAGLNVLYDSIDNPRKPTMGIRSSLNFELSGLGGTYQFTKLTA SGSIYRLLTKKGVLKIRGEAKFIKPFGTTTAQGIPVSERFFLGGESTVRGYKPFIIGPKFSPTEPQGGLS SLLLTEEFQYPLISQPSINAFVFLDSGFIGTEEYTIRLKNLCSSAGFGLRFDMMNNVPIMLGWGWPFRPT EILNNEKIDVSQRFFFALGGVF Protective antigen Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0512 showed significant levels of protection when compared to negative control animals. At 9 days following challenge TC0512 showed a 73% reduction in the number of recoverable Chlamydia compared with vector only immunized controls [Ref1057:McNeilly et al., 2007]. TC0757 hypothetical protein Chlamydia muridarum Nigg VO_0010886 1246122 15835371 TC0757 NZ_ACOV01000003 NP_297130 243161 898853 899647 + hypothetical protein 5.02 27979.63 264 identified by match to TIGR protein family HMM TIGR01607 >gi|29337300:898853-899647 Chlamydia muridarum Nigg, complete genome CATGCGCTGTTCTGCTTATTGCTCTGCTTCGTCCTATCATCTACACGTTCTTTTTCATCTTTTAAAGGCA CGCTACCCTTCTGTCTTATCCCGAGAATACGTGCTCATTTCCTCTGAAGAGCTGAATGAAAGCGACAAAG CCGCTGTCTTTTTCCCATTTGGAGTTAGTGTGTTTTGGGGATGGGAAGAAGCTGAAGAACTTCAAGTGAT CCAAGCTATCACCTCAGCATCAGTGGATCCTCTCCCTAACCCAGAAATTGATAGTTATGACTTCCATTAT GGAGAGAAACTACAGATCCGTCGCGATAGACTTGTTCTCACCAACTCTAATTTAAACACAAAACTTGCTA TTTCCTTTGGGTTAGCCCAATCTATCAAACTTACCGTCTTTGAAGAAACGATTTACAAAACAGTTGAAAA CTCTAAATCTTTGCCTCAAGAACTTGCCTCTAAAGGGAAAATTTCTCTATCCCGTAAAACGATCGCAAAA AAGATTGGAGAGCTTTTCCTGGATAAAGCTTCCGTAAACCTACACTCTGACATTCTCGATGAGCCCGACT TTTTCTGGGAACATCCAGAAACCCAACCTTTTTATATTGATGTGTTGACCTGCCTAGATGTAAACGCGCG AGTAAATGTATTAAATCACAGACTTGCTATCCTAGGAGATGTTCTCGAGATTTTAAATGACCAACTTAAT CATCAGCATTCTTCAGCTCTAGAGTGGACCGTGATCTGGTTAATTGCACTAGAGGTATTGGTCACCTTAC TTAAAGACGTATTCAACATCATCTA >gi|15835371|ref|NP_297130.1| hypothetical protein TC0757 [Chlamydia muridarum Nigg] MRCSAYCSASSYHLHVLFHLLKARYPSVLSREYVLISSEELNESDKAAVFFPFGVSVFWGWEEAEELQVI QAITSASVDPLPNPEIDSYDFHYGEKLQIRRDRLVLTNSNLNTKLAISFGLAQSIKLTVFEETIYKTVEN SKSLPQELASKGKISLSRKTIAKKIGELFLDKASVNLHSDILDEPDFFWEHPETQPFYIDVLTCLDVNAR VNVLNHRLAILGDVLEILNDQLNHQHSSALEWTVIWLIALEVLVTLLKDVFNII Protective antigen Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0757 showed significant levels of protection when compared to negative control animals [Ref1057:McNeilly et al., 2007]. TC0767 hypothetical protein Chlamydia muridarum Nigg VO_0010888 1246132 15835381 TC0767 AE002160 NP_297140 243161 910619 911350 - hypothetical protein 9.14 26828.18 243 >gi|29337300:910619-911350 Chlamydia muridarum Nigg, complete genome ATTAATGCAAAATAAGGAATCGTTTTAATTGGCGGAAAGAGAGCTGATAAAGAGATGGGATCCCTCGACT ATCTAAAGCCATATCAATACGAGGAATGCTATGCTGTGATTGCATCGAGTAAAAAAAACTCGCAGCATCC CCTAACACAAAAGAATAGCGGTAAACCTCCTGAACAAAATGTTGACTCACCAAATCACTTTTTCCAAAAG GATAAAAAAAGACTTCCACAGGAGCCTGTATAGCCTCTTCTAGTAAAATTTTAGACAATGAAATTTCAGT ATGTAAATAAGGAGGGGAGTTTTTAAGGTTTCTAATAGCAAACCCTGAAGAGGCAAATCGAATAATCGAA CTTTGAGCCATATGACAAAGTTCTTTAACCGAACAAAAGGGCTGATAATAAGAAAATATTTCGTCTTGAA AAGCTAGAAAATCCGATGGCGAAATTCGCATATCAATGGGAAGATTTTCACTTTCTAAACGGGATACGTA TCTCCAAGCAACTCCAACAACCGCAGGAATCTGGAATTTCTGAAGAAAAGGAAAGACATGCTTGTAAAAA TCAACAGAAGCATAATCGAATGTCAGTATGATTGCTTTCCTATTAGGGAGAGGATCTCCGGGCAGAATAA ACGAATAATGCTGCTTGAGAGAGTGCAAAAAATCTAAAAACGATCTGAGGGCATAAGGAAACTTAGAAAA AGCAACCTGTCGATAGGCTAAGACGCGGAGCA >gi|15835381|ref|NP_297140.1| hypothetical protein TC0767 [Chlamydia muridarum Nigg] MLRVLAYRQVAFSKFPYALRSFLDFLHSLKQHYSFILPGDPLPNRKAIILTFDYASVDFYKHVFPFLQKF QIPAVVGVAWRYVSRLESENLPIDMRISPSDFLAFQDEIFSYYQPFCSVKELCHMAQSSIIRFASSGFAI RNLKNSPPYLHTEISLSKILLEEAIQAPVEVFFYPFGKSDLVSQHFVQEVYRYSFVLGDAASFFYSMQSQ HSIPRIDMALDSRGIPSLYQLSFRQLKRFLILH Protective antigen Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals [Ref1057:McNeilly et al., 2007]. TC0768 hypothetical protein Chlamydia muridarum Nigg VO_0010889 1246133 15835382 TC0768 AE002160 NP_297141 243161 911335 911496 - hypothetical protein 3.69 5224.4 53 >gi|29337300:911335-911496 Chlamydia muridarum Nigg, complete genome GCTAAGACGCGGAGCATGGATCAACTGAAGGAATTGCAAAAGGGTCCTCTGGATAAATGACTACCTGTCC GGAAATGATCGTTAGATTACGCTCTTTTCCCTCAGGAGTAAGCTCTGCTGTTTCCGTAGAACAGACTTCA GAAGTAGGTAAGGAGATCGGCA >gi|15835382|ref|NP_297141.1| hypothetical protein TC0768 [Chlamydia muridarum Nigg] MPISLPTSEVCSTETAELTPEGKERNLTIISGQVVIYPEDPFAIPSVDPCSAS Protective antigen Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals [Ref1057:McNeilly et al., 2007]. Faludi et al., 2009 journal Faludi I, Burian K, Csanadi A, Miczak A, Lu X, Kakkar VV, Gonczol E, Endresz V 2009 299 7 520-528 International journal of medical microbiology : IJMM McNeilly et al., 2007 journal McNeilly CL, Beagley KW, Moore RJ, Haring V, Timms P, Hafner LM 2007 25 14 2643-2655 Vaccine Skelding et al., 2006 journal Skelding KA, Hickey DK, Horvat JC, Bao S, Roberts KG, Finnie JM, Hansbro PM, Beagley KW 2006 24 3 355-366 Vaccine Wiki: Chlamydia muridarum website http://en.wikipedia.org/wiki/Chlamydia_muridarum Yu et al., 2009 journal Yu H, Jiang X, Shen C, Karunakaran KP, Brunham RC 2009 182 3 1602-1608 Journal of immunology (Baltimore, Md. : 1950)