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Vaccine Comparison
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Bordetella pertussis aroA mutant vaccine |
Bordetella pertussis dnt mutant vaccine |
| Vaccine Information |
Vaccine Information |
- Vaccine Ontology ID: VO_0002807
- Type: Live, attenuated vaccine
- Status: Research
- aroA
gene engineering:
- Type: Gene mutation
- Detailed Gene Information: Click Here.
- Preparation: A DNA fragment encoding a kanamycin resistance determinant was used to insertionally inactivate the cloned aroA gene of Bordetella pertussis in Escherichia coli K-12, and a conjugative shuttle vector system based on the suicide vector pRTP1 was used to deliver the mutations from E. coli back into B. pertussis CN2992FS and BP1. The aroA mutation was introduced by allelic exchange into the chromosome of B. pertussis, resulting in otherwise isogenic parental and aroA mutant pairs. The B. pertussis aroA mutants grew well on laboratory medium supplemented with aromatic compounds but failed to grow on unsupplemented medium (Roberts et al., 1990).
- Immunization Route: intracerebral challenge
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- Vaccine Ontology ID: VO_0002808
- Type: Live, attenuated vaccine
- Status: Research
- dnt
gene engineering:
- Type: Gene mutation
- Detailed Gene Information: Click Here.
- Preparation: his strain was obtained by genetically inactivating pertussis toxin via two point mutations in the ptx gene, by deleting dnt encoding dermonecrotic toxin, and by replacing the B. pertussis ampG gene by Escherichia coli ampG, resulting in the removal of tracheal cytotoxin (Feunou et al., 2008).
- Immunization Route: intranasal immunization
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| References |
References |
Roberts et al., 1990: Roberts M, Maskell D, Novotny P, Dougan G. Construction and characterization in vivo of Bordetella pertussis aroA mutants. Infection and immunity. 1990; 58(3); 732-739. [PubMed: 2407655].
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Feunou et al., 2008: Feunou PF, Ismaili J, Debrie AS, Huot L, Hot D, Raze D, Lemoine Y, Locht C. Genetic stability of the live attenuated Bordetella pertussis vaccine candidate BPZE1. Vaccine. 2008; 26(45); 5722-5727. [PubMed: 18762220].
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