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M. tuberculosis phoP mutant SO2 |
| Vaccine Information |
- Tradename: M. tuberculosis phoP mutant strain
- Vaccine Ontology ID: VO_0000588
- Type: Live attenuated vaccine
- PhoP
gene engineering:
- Type: Gene mutation
- Description: The mutant strain was constructed by a single gene (phoP) disruption of the parental M. tuberculosis MT103 strain. PhoP has shown involvement in the regulation of complex mycobacterial lipids implicated in the virulence of M. tuberculosis (Martin et al., 2006).
- Detailed Gene Information: Click Here.
- Preparation: Rabbits were treated with four doses of phoP prior to isolating polyclonal antibodies against phoP. Anti-phoP and monoclonal antibodies against ESAT-6 were obtained. Horseradish peroxidase-labelled goat anti-rabbit antibodies served as secondary antibodies (Martin et al., 2006). Mycobacterial cell-free protein extracts from M. tuberculosis were prepared, filtered, and precipitated with 45% (w/v) ammonium sulphate (Martin et al., 2006).
- Description: The phoP transcription factor is part of the two-component regulatory signal transduction system component which recently was linked to regulation of complex mycobacterial lipids implicated in the virulence of M. tuberculosis, as well as to multidrug resistance occurrences in a clinical isolate (Martin et al., 2006; Perez et al., 2001) established the selection of phoP as a vaccine candidate by showing that the mutant SO2 strain shows impaired multiplication within cultured macrophages and in vivo infection models (Martin et al., 2006).
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| References |
Martin et al., 2006: Martin C, Williams A, Hernandez-Pando R, Cardona PJ, Gormley E, Bordat Y, Soto CY, Clark SO, Hatch GJ, Aguilar D, Ausina V, Gicquel B. The live Mycobacterium tuberculosis phoP mutant strain is more attenuated than BCG and confers protective immunity against tuberculosis in mice and guinea pigs. Vaccine. 2006 Apr 24; 24(17); 3408-19. [PubMed: 16564606 ].
Perez et al., 2001: Perez E, Samper S, Bordas Y, Guilhot C, Gicquel B, Martin C. An essential role for phoP in Mycobacterium tuberculosis virulence. Molecular microbiology. 2001 Jul; 41(1); 179-87. [PubMed: 11454210].
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M. tuberculosis secA2 mutant |
| Vaccine Information |
- Vaccine Ontology ID: VO_0000564
- Type: Live, attenuated vaccine
- SecA2
gene engineering:
- Type: secA2 transformation into M. tuberculosis
- Description: The secA2 gene was amplified by PCR from virulent H37Rv genomic DNA, cloned into a pGEM vector, and later excised as a fragment and cloned into pMV361 (Hinchey et al., 2007).
- Detailed Gene Information: Click Here.
- Preparation: Three virulent strains (M. tuberculosis H37Rv, HN878, and ΔsecA2 mutants) were obtained and grown on Middlebrook 7H9 broth or 7H10 agar containing 10% (vol/vol) oleic acid-albumin-dextrose-catalase, 0.5% glycerol, and 0.05% (vol/vol) Tween-80. Recombinant strains were selected using ampicillin, hygromycin, and kanamycin.
- Description: The secA2 gene encodes a component of a virulence-associated bacterial protein secretion system and is required for prevention of apoptosis in macrophages infected with M. tuberculosis. The apoptotic response is related to secA2-dependent secretion of mycobacterial superoxide dismutase A (SodA). M. tuberculosis H37Rv with deleted secA2 induces apoptosis, as indicated by TUNEL and polycaspase activation in mouse and human macrophages (Hinchey et al., 2007). Extrinsic and intrinsic caspase pathways were linked to initiation of apoptosis in the secA2-deleted mutants via caspase 8 and 9 activity. The secretion of SodA appears to be important for the inhibition of apoptosis by secA2-dependent mechanisms. Furthermore,
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| References |
Hinchey et al., 2007: Hinchey J, Lee S, Jeon BY, Basaraba RJ, Venkataswamy MM, Chen B, Chan J, Braunstein M, Orme IM, Derrick SC, Morris SL, Jacobs WR, Porcelli SA. Enhanced priming of adaptive immunity by a proapoptotic mutant of Mycobacterium tuberculosis. The Journal of clinical investigation. 2007 Aug 1; 117(8); 2279-2288. [PubMed: 17671656].
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Mycobacterium tuberculosis DeltasigF mutant vaccine |
| Vaccine Information |
- Type: Live, attenuated vaccine
- Status: Research
- Host Species for Licensed Use: None
- Host Species as Laboratory Animal Model: Rabbit
- sigF
gene engineering:
- Type: Gene mutation
- Detailed Gene Information: Click Here.
- Immunization Route: Intradermal injection (i.d.)
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| References |
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Mycobacterium tuberculosis drrC mutant vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0002780
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- drrC
gene engineering:
- Type: Gene mutation
- Description: This drrC mutant is from Mycobacterium tuberculosis (Pinto et al., 2004).
- Detailed Gene Information: Click Here.
- Immunization Route: Intravenous injection (i.v.)
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| References |
Pinto et al., 2004: Pinto R, Saunders BM, Camacho LR, Britton WJ, Gicquel B, Triccas JA. Mycobacterium tuberculosis defective in phthiocerol dimycocerosate translocation provides greater protective immunity against tuberculosis than the existing bacille Calmette-Guérin vaccine. The Journal of infectious diseases. 2004; 189(1); 105-112. [PubMed: 14702160].
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Mycobacterium tuberculosis fadD26 mutant vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0002781
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- fadD26
gene engineering:
- Type: Gene mutation
- Description: This fadD26 mutant is from Mycobacterium tuberculosis (Infante et al., 2005).
- Detailed Gene Information: Click Here.
- Immunization Route: Intratracheal immunization
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| References |
Infante et al., 2005: Infante E, Aguilar LD, Gicquel B, Pando RH. Immunogenicity and protective efficacy of the Mycobacterium tuberculosis fadD26 mutant. Clinical and experimental immunology. 2005; 141(1); 21-28. [PubMed: 15958066].
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Mycobacterium tuberculosis leuD mutant vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0002782
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- leuD
gene engineering:
- Type: Gene mutation
- Description: This leuD mutant is from Mycobacterium tuberculosis (Hondalus et al., 2000).
- Detailed Gene Information: Click Here.
- Immunization Route: Intravenous injection (i.v.)
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| References |
Hondalus et al., 2000: Hondalus MK, Bardarov S, Russell R, Chan J, Jacobs WR Jr, Bloom BR. Attenuation of and protection induced by a leucine auxotroph of Mycobacterium tuberculosis. Infection and immunity. 2000; 68(5); 2888-2898. [PubMed: 10768986].
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Mycobacterium tuberculosis lysA/secA2 mutant vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0002783
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- lysA
gene engineering:
- Type: Gene mutation
- Description: This lysA/secA2 mutant is from Mycobacterium tuberculosis (Hinchey et al., 2011).
- Detailed Gene Information: Click Here.
- SecA2
gene engineering:
- Type: Gene mutation
- Description: This lysA/secA2 mutant is from Mycobacterium tuberculosis (Hinchey et al., 2011).
- Detailed Gene Information: Click Here.
- Immunization Route: subcutaneous injection
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| References |
Hinchey et al., 2011: Hinchey J, Jeon BY, Alley H, Chen B, Goldberg M, Derrick S, Morris S, Jacobs WR Jr, Porcelli SA, Lee S. Lysine auxotrophy combined with deletion of the SecA2 gene results in a safe and highly immunogenic candidate live attenuated vaccine for tuberculosis. PloS one. 2011; 6(1); e15857. [PubMed: 21264335].
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Mycobacterium tuberculosis mce-2 mutant vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004279
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- mce-2
gene engineering:
- Type: Gene mutation
- Description: This mce-2 mutant is from Mycobacterium tuberculosis (Aguilar et al., 2006).
- Detailed Gene Information: Click Here.
- mce-3
gene engineering:
- Type: Gene mutation
- Description: This mce-2/mce-3 mutant is from Mycobacterium tuberculosis (Aguilar et al., 2006).
- Detailed Gene Information: Click Here.
- Immunization Route: subcutaneous injection
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| References |
Aguilar et al., 2006: Aguilar LD, Infante E, Bianco MV, Cataldi A, Bigi F, Pando RH. Immunogenicity and protection induced by Mycobacterium tuberculosis mce-2 and mce-3 mutants in a Balb/c mouse model of progressive pulmonary tuberculosis. Vaccine. 2006; 24(13); 2333-2342. [PubMed: 16388878].
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Mycobacterium tuberculosis mce-3 mutant vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0004288
- Type: Live, attenuated vaccine
- Status: Research
- Host Species for Licensed Use: Mouse
- mce-3
gene engineering:
- Type: Gene mutation
- Description: This mce-3 mutant is from Mycobacterium tuberculosis (Aguilar et al., 2006).
- Detailed Gene Information: Click Here.
- Immunization Route: subcutaneous injection
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| References |
Aguilar et al., 2006: Aguilar LD, Infante E, Bianco MV, Cataldi A, Bigi F, Pando RH. Immunogenicity and protection induced by Mycobacterium tuberculosis mce-2 and mce-3 mutants in a Balb/c mouse model of progressive pulmonary tuberculosis. Vaccine. 2006; 24(13); 2333-2342. [PubMed: 16388878].
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Mycobacterium tuberculosis panCD mutant vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0002786
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- panC
gene engineering:
- Type: Gene mutation
- Description: This panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2002).
- Detailed Gene Information: Click Here.
- panD
gene engineering:
- Type: Gene mutation
- Description: This panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2002).
- Detailed Gene Information: Click Here.
- Immunization Route: subcutaneous injection
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| References |
Sambandamurthy et al., 2002: Sambandamurthy VK, Wang X, Chen B, Russell RG, Derrick S, Collins FM, Morris SL, Jacobs WR Jr. A pantothenate auxotroph of Mycobacterium tuberculosis is highly attenuated and protects mice against tuberculosis. Nature medicine. 2002; 8(10); 1171-1174. [PubMed: 12219086].
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Mycobacterium tuberculosis proC mutant vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0002791
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- proC
gene engineering:
- Type: Gene mutation
- Description: This proC mutant is from Mycobacterium tuberculosis (Smith et al., 2001).
- Detailed Gene Information: Click Here.
- Immunization Route: Intravenous injection (i.v.)
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| References |
Smith et al., 2001: Smith DA, Parish T, Stoker NG, Bancroft GJ. Characterization of auxotrophic mutants of Mycobacterium tuberculosis and their potential as vaccine candidates. Infection and immunity. 2001; 69(2); 1142-1150. [PubMed: 11160012].
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Mycobacterium tuberculosis RD1/panCD mutant vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0002787
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- RD1
gene engineering:
- Type: Gene mutation
- Description: This RD1/panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2006).
- Detailed Gene Information: Click Here.
- panC
gene engineering:
- Type: Gene mutation
- Description: This RD1/panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2006).
- Detailed Gene Information: Click Here.
- panD
gene engineering:
- Type: Gene mutation
- Description: This RD1/panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2006).
- Detailed Gene Information: Click Here.
- Immunization Route: subcutaneous injection
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| References |
Sambandamurthy et al., 2006: Sambandamurthy VK, Derrick SC, Hsu T, Chen B, Larsen MH, Jalapathy KV, Chen M, Kim J, Porcelli SA, Chan J, Morris SL, Jacobs WR Jr. Mycobacterium tuberculosis DeltaRD1 DeltapanCD: a safe and limited replicating mutant strain that protects immunocompetent and immunocompromised mice against experimental tuberculosis. Vaccine. 2006; 24(37-39); 6309-6320. [PubMed: 16860907].
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Mycobacterium tuberculosis sigE mutant vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0002795
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- sigE
gene engineering:
- Type: Gene mutation
- Description: This sigE mutant is from Mycobacterium tuberculosis (Hernandez et al., 2010).
- Detailed Gene Information: Click Here.
- Immunization Route: Intratracheal immunization
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| References |
Hernandez et al., 2010: Hernandez Pando R, Aguilar LD, Smith I, Manganelli R. Immunogenicity and protection induced by a Mycobacterium tuberculosis sigE mutant in a BALB/c mouse model of progressive pulmonary tuberculosis. Infection and immunity. 2010; 78(7); 3168-3176. [PubMed: 20457786].
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Mycobacterium tuberculosis trpD mutant vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0002796
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- trpD
gene engineering:
- Type: Gene mutation
- Description: This trpD mutant is from Mycobacterium tuberculosis (Smith et al., 2001).
- Detailed Gene Information: Click Here.
- Immunization Route: Intravenous injection (i.v.)
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| References |
Smith et al., 2001: Smith DA, Parish T, Stoker NG, Bancroft GJ. Characterization of auxotrophic mutants of Mycobacterium tuberculosis and their potential as vaccine candidates. Infection and immunity. 2001; 69(2); 1142-1150. [PubMed: 11160012].
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