Francisella tularensis capB mutant vaccine |
Vaccine Information |
- Vaccine Ontology ID: VO_0002840
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- capB
gene engineering:
- Type: Gene mutation
- Description: This capB mutant is from Francisella tularensis (Jia et al., 2010).
- Detailed Gene Information: Click Here.
- Immunization Route: intranasal immunization
|
References |
Jia et al., 2010: Jia Q, Lee BY, Bowen R, Dillon BJ, Som SM, Horwitz MA. A Francisella tularensis live vaccine strain (LVS) mutant with a deletion in capB, encoding a putative capsular biosynthesis protein, is significantly more attenuated than LVS yet induces potent protective immunity in mice against F. tularensis challenge. Infection and immunity. 2010; 78(10); 4341-4355. [PubMed: 20643859].
|
Francisella tularensis clpB mutant vaccine |
Vaccine Information |
- Vaccine Ontology ID: VO_0002841
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- clpB
gene engineering:
- Type: Gene mutation
- Description: This clpB mutant is from Francisella tularensis (Conlan et al., 2010).
- Detailed Gene Information: Click Here.
- Immunization Route: Intradermal injection (i.d.)
|
References |
Conlan et al., 2010: Conlan JW, Shen H, Golovliov I, Zingmark C, Oyston PC, Chen W, House RV, Sjöstedt A. Differential ability of novel attenuated targeted deletion mutants of Francisella tularensis subspecies tularensis strain SCHU S4 to protect mice against aerosol challenge with virulent bacteria: effects of host background and route of immunization. Vaccine. 2010; 28(7); 1824-1831. [PubMed: 20018266].
|
Francisella tularensis FTL0552 mutant vaccine |
Vaccine Information |
- Vaccine Ontology ID: VO_0002842
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- FTL0552
gene engineering:
- Immunization Route: intranasal immunization
|
References |
Sammons-Jackson et al., 2008: Sammons-Jackson WL, McClelland K, Manch-Citron JN, Metzger DW, Bakshi CS, Garcia E, Rasley A, Anderson BE. Generation and characterization of an attenuated mutant in a response regulator gene of Francisella tularensis live vaccine strain (LVS). DNA and cell biology. 2008; 27(7); 387-403. [PubMed: 18613792].
|
Francisella tularensis FTT0918 mutant vaccine |
Vaccine Information |
- Vaccine Ontology ID: VO_0002843
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- FTT0918
gene engineering:
- Type: Gene mutation
- Description: This FTT0918 mutant is from Francisella tularensis (Twine et al., 2005).
- Detailed Gene Information: Click Here.
- Immunization Route: Intradermal injection (i.d.)
|
References |
Twine et al., 2005: Twine S, Bystrom M, Chen W, Forsman M, Golovliov I, Johansson A, Kelly J, Lindgren H, Svensson K, Zingmark C, Conlan W, Sjostedt A. A mutant of Francisella tularensis strain SCHU S4 lacking the ability to express a 58-kilodalton protein is attenuated for virulence and is an effective live vaccine. Infection and immunity. 2005 Dec; 73(12); 8345-52. [PubMed: 16299332 ].
|
Francisella tularensis galU mutant vaccine |
Vaccine Information |
- Vaccine Ontology ID: VO_0002844
- Type: Live, attenuated vaccine
- Status: Licensed
- Host Species as Laboratory Animal Model: Mouse
- galU
gene engineering:
- Type: Gene mutation
- Description: This galU mutant is from Francisella tularensis (Jayakar et al., 2011).
- Detailed Gene Information: Click Here.
- Immunization Route: intranasal immunization
|
References |
Jayakar et al., 2011: Jayakar HR, Parvathareddy J, Fitzpatrick EA, Bina XR, Bina JE, Re F, Emery FD, Miller MA. A galU Mutant of Francisella tularensis is Attenuated for Virulence in a Murine Pulmonary Model of Tularemia. BMC microbiology. 2011; 11(1); 179. [PubMed: 21819572].
|
Francisella tularensis guaA mutant vaccine |
Vaccine Information |
- Vaccine Ontology ID: VO_0002845
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- guaA
gene engineering:
- Type: Gene mutation
- Description: This guaA mutant is from Francisella tularensis (Santiago et al., 2009).
- Detailed Gene Information: Click Here.
- Immunization Route: Intraperitoneal injection (i.p.)
|
References |
Santiago et al., 2009: Santiago AE, Cole LE, Franco A, Vogel SN, Levine MM, Barry EM. Characterization of rationally attenuated Francisella tularensis vaccine strains that harbor deletions in the guaA and guaB genes. Vaccine. 2009; 27(18); 2426-2436. [PubMed: 19368784].
|
Francisella tularensis guaB mutant vaccine |
Vaccine Information |
- Vaccine Ontology ID: VO_0002846
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- guaB
gene engineering:
- Type: Gene mutation
- Description: This guaB mutant is from Francisella tularensis (Santiago et al., 2009).
- Detailed Gene Information: Click Here.
- Immunization Route: Intraperitoneal injection (i.p.)
|
References |
Santiago et al., 2009: Santiago AE, Cole LE, Franco A, Vogel SN, Levine MM, Barry EM. Characterization of rationally attenuated Francisella tularensis vaccine strains that harbor deletions in the guaA and guaB genes. Vaccine. 2009; 27(18); 2426-2436. [PubMed: 19368784].
|
Francisella tularensis IglB mutant vaccine |
Vaccine Information |
- Product Name: KKF235
- Vaccine Ontology ID: VO_0002847
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- IglB
gene engineering:
- Type: Gene mutation
- Description: This IgIB mutant is from Francisella tularensis (Yu et al., 2010).
- Detailed Gene Information: Click Here.
- Immunization Route: Oral immunization
|
References |
Yu et al., 2010: Yu JJ, Goluguri T, Guentzel MN, Chambers JP, Murthy AK, Klose KE, Forsthuber TG, Arulanandam BP. Francisella tularensis T-cell antigen identification using humanized HLA-DR4 transgenic mice. Clinical and vaccine immunology : CVI. 2010; 17(2); 215-222. [PubMed: 20016043].
|
Francisella tularensis purF mutant vaccine |
Vaccine Information |
- Vaccine Ontology ID: VO_0002850
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- purF
gene engineering:
- Type: Gene mutation
- Description: This purF mutant is from Francisella tularensis (Quarry et al., 2007).
- Detailed Gene Information: Click Here.
- Immunization Route: Intraperitoneal injection (i.p.)
|
References |
Quarry et al., 2007: Quarry JE, Isherwood KE, Michell SL, Diaper H, Titball RW, Oyston PC. A Francisella tularensis subspecies novicida purF mutant, but not a purA mutant, induces protective immunity to tularemia in mice. Vaccine. 2007; 25(11); 2011-2018. [PubMed: 17241711].
|
Francisella tularensis purMCD mutant vaccine |
Vaccine Information |
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- PurC/PurD
gene engineering:
- Type: Gene mutation
- Description: This purMCD mutant is from Francisella tularensis (Pechous et al., 2006).
- Detailed Gene Information: Click Here.
- purM
gene engineering:
- Type: Gene mutation
- Description: This purMCD mutant is from Francisella tularensis (Pechous et al., 2006).
- Detailed Gene Information: Click Here.
- Immunization Route: Intraperitoneal injection (i.p.)
|
References |
Pechous et al., 2006: Pechous R, Celli J, Penoske R, Hayes SF, Frank DW, Zahrt TC. Construction and characterization of an attenuated purine auxotroph in a Francisella tularensis live vaccine strain. Infection and immunity. 2006; 74(8); 4452-4461. [PubMed: 16861631].
|
Francisella tularensis sodC mutant vaccine |
Vaccine Information |
- Vaccine Ontology ID: VO_0002853
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- sodC
gene engineering:
- Type: Gene mutation
- Description: This sodC mutant is from Francisella tularensis (Melillo et al., 2009).
- Detailed Gene Information: Click Here.
- Immunization Route: intranasal immunization
|
References |
Melillo et al., 2009: Melillo AA, Mahawar M, Sellati TJ, Malik M, Metzger DW, Melendez JA, Bakshi CS. Identification of Francisella tularensis live vaccine strain CuZn superoxide dismutase as critical for resistance to extracellularly generated reactive oxygen species. Journal of bacteriology. 2009; 191(20); 6447-6456. [PubMed: 19684141].
|
Francisella tularensis wbtA mutant vaccine |
Vaccine Information |
- Vaccine Ontology ID: VO_0002854
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- wbtA
gene engineering:
- Type: Gene mutation
- Description: This wbtA mutant is from Francisella tularensis (Sebastian et al., 2007).
- Detailed Gene Information: Click Here.
- Immunization Route: Intradermal injection (i.d.)
|
References |
Sebastian et al., 2007: Sebastian S, Dillon ST, Lynch JG, Blalock LT, Balon E, Lee KT, Comstock LE, Conlan JW, Rubin EJ, Tzianabos AO, Kasper DL. A defined O-antigen polysaccharide mutant of Francisella tularensis live vaccine strain has attenuated virulence while retaining its protective capacity. Infection and immunity. 2007; 75(5); 2591-2602. [PubMed: 17296751].
|
Francisella tularensis wbtI mutant vaccine |
Vaccine Information |
- Vaccine Ontology ID: VO_0002855
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- WbtI
gene engineering:
- Type: Gene mutation
- Description: This wbtI mutant is from Francisella tularensis (Li et al., 2007).
- Detailed Gene Information: Click Here.
- Immunization Route: Intraperitoneal injection (i.p.)
|
References |
Li et al., 2007: Li J, Ryder C, Mandal M, Ahmed F, Azadi P, Snyder DS, Pechous RD, Zahrt T, Inzana TJ. Attenuation and protective efficacy of an O-antigen-deficient mutant of Francisella tularensis LVS. Microbiology (Reading, England). 2007; 153(Pt 9); 3141-3153. [PubMed: 17768257].
|
KKF24 |
Vaccine Information |
- Vaccine Ontology ID: VO_0000307
- Type: Live Attenuated
- IglC
gene engineering:
- Type: Protein
- Detailed Gene Information: Click Here.
- Preparation: KKF24 is a recombinant strain of Francisella novicida U112 with iglC mutation (F. novicida delta-iglC::ermC) (Lauriano et al., 2003). Strains were grown at 37°C in Trypticase soy broth supplemented with 0.1% cysteine (Pammit et al., 2006).
- Virulence: KKF24 is highly attenuated for virulence in mice and growth within amoebae (Pammit et al., 2006).
- Description: KKF24 has been identified as a live attenuated vaccine against subsequent intranasal (i.n.) challenge with the wild-type organism of Francisella tularensis (Pammit et al., 2006).
|
References |
Lauriano et al., 2003: Lauriano CM, Barker JR, Nano FE, Arulanandam BP, Klose KE. Allelic exchange in Francisella tularensis using PCR products. FEMS microbiology letters. 2003 Dec 12; 229(2); 195-202. [PubMed: 14680699].
Pammit et al., 2006: Pammit MA, Raulie EK, Lauriano CM, Klose KE, Arulanandam BP. Intranasal vaccination with a defined attenuated Francisella novicida strain induces gamma interferon-dependent antibody-mediated protection against tularemia. Infection and immunity. 2006 Apr; 74(4); 2063-71. [PubMed: 16552035].
|
sodB(Ft) |
Vaccine Information |
- Product Name: LVS sodB mutant
- Vaccine Ontology ID: VO_0000263
- Type: Live Attenuated
- SodB from F. tularensis SCHU S4
gene engineering:
- Type: Protein
- Detailed Gene Information: Click Here.
- Preparation: sodBFt is made by amplifying a region of the sodB gene by PCR. The full-length sodB gene is also amplified. These amplified fragments are cloned separately into a PCR cloning vector and excised by digestion with restriction enzymes. The digested fragments are ligated simultaneously to an XbaI/SalI-digested pPV shuttle vector to yield pPV-sodB. The PstI site upstream of the start codon is used as a marker for screening of mutant colonies. pPV-sodB is then transformed in Escherichia coli S17-1 and transferred into F. tularensis LVS via conjugation. The mutants are selected on modified chocolate agar plates supplemented with Isovitalex, L-cysteine hydrochloride, 1% hemoglobin, 2 µg/ml chloramphenicol, and 100 µg/ml polymyxin B. Colonies obtained on the chloramphenicol plates following the first recombination event are analyzed by PCR, followed by the digestion of PCR products with PstI enzyme. The positive mutant colonies are selected for a second recombination event by plating on medium containing 5% sucrose. Chloramphenicol sensitivity and sucrose resistance following the second recombination event are confirmed by PCR and PstI digestion of the amplified products. The positive mutant clone is referred to as sodBFt (Bakshi et al., 2006).
- Virulence: sodB(Ft) has increased sensitivity to paraquat and hydrogen peroxide (H2O2), the redox cycling compounds, as well as with reduced virulence in both C57BL/6 and BALB/c mice (Bakshi et al., 2006).
- Description: sodBFt is a live vaccine strain mutant of Francisella tularensis with reduced Fe-superoxide dismutase gene expression (Bakshi et al., 2006).
|
References |
Bakshi et al., 2006: Bakshi CS, Malik M, Regan K, Melendez JA, Metzger DW, Pavlov VM, Sellati TJ. Superoxide dismutase B gene (sodB)-deficient mutants of Francisella tularensis demonstrate hypersensitivity to oxidative stress and attenuated virulence. Journal of bacteriology. 2006 Sep; 188(17); 6443-8. [PubMed: 16923916].
|