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Vaccine Detail
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Leishmania infantum HSP70 II mutant vaccine |
| Vaccine Information |
- Vaccine Ontology ID: VO_0003006
- Type: Live, attenuated vaccine
- Status: Research
- HSP70 II
gene engineering:
- Type: Gene mutation
- Detailed Gene Information: Click Here.
- Preparation: The HSP70-II null mutant (∆hsp70-II::NEO/∆hsp70-II::HYG) is a cloned line that was generated by targeted deletion of both HSP70-II alleles in the L. infantum strain MCAN/ES/96/BCN150 [32]. L. major promastigote (strain MHOM/IL/80/Friedlin; clon V1) were also used in this study. Promastigotes of both species were grown in RPMI 1640 culture medium supplemented with 10% heatinactivated FBS, 100 U/ml penicillin and 100 µg/ml streptomycin (Carrion et al., 2011).
- Immunization Route: Intraperitoneal injection (i.p.)
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| References |
Carrion et al., 2011: Carrion J, Folgueira C, Soto M, Fresno M, Requena JM. Leishmania infantum HSP70-II null mutant as candidate vaccine against leishmaniasis: a preliminary evaluation. Parasites & vectors. 2011; 4(1); 150. [PubMed: 21794145].
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