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Vaccine Comparison
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Influenza virus CTA1-3M2e-DD protein vaccine |
Influenza virus NS1 mutant vaccine |
| Vaccine Information |
Vaccine Information |
- Vaccine Ontology ID: VO_0002980
- Type: Subunit vaccine
- Status: Research
- Antigen: Influenza A virus (A/Puerto Rico/8/34(H1N1)) matrix protein 2 (M2)
- M2 from Influenza A virus (A/Puerto Rico/8/34(H1N1))
gene engineering:
- Type: Recombinant protein preparation
- Description: The fusion proteins were expressed in E. coli DH5 cells, transformed with the expression vectors for the CTA1-DD, CTA1-M2e-DD or the CTA1-3M2e-DD fusion proteins, and grown in 500 ml cultures over night in SYPPG medium with 100 μg/ml carbenicillin, at 37 °C. The cells were harvested by centrifugation and the fusion proteins, produced as inclusion bodies, were washed before extraction by treatment with 8 M urea. After refolding the proteins by slowly diluting them 35–40x in Tris–HCl pH 7.4 at +4 °C, the fusion proteins were purified in two steps, by ion exchange and size exclusion chromatography. After concentration and sterile filtration the purified fusion proteins were stored in PBS at −80 °C until use (Eliasson et al., 2008).
- Detailed Gene Information: Click Here.
- Adjuvant: cholera toxin A1-subunit-ProteinA D-fragment fusion protein
- Immunization Route: Intranasal
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- Vaccine Ontology ID: VO_0002981
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse, pig, horse, macaque
- NS1 from Influenza virus
gene engineering:
- Immunization Route: intranasal immunization
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| References |
References |
Eliasson et al., 2008: Eliasson DG, El Bakkouri K, Schön K, Ramne A, Festjens E, Löwenadler B, Fiers W, Saelens X, Lycke N. CTA1-M2e-DD: a novel mucosal adjuvant targeted influenza vaccine. Vaccine. 2008; 26(9); 1243-1252. [PubMed: 18243429].
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Richt and GarcÃa-Sastre, 2009: Richt JA, GarcÃa-Sastre A. Attenuated influenza virus vaccines with modified NS1 proteins. Current topics in microbiology and immunology. 2009; 333; 177-195. [PubMed: 19768406].
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