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Apa
Gene Name	Apa
Sequence Strain (Species/Organism)	Mycobacterium tuberculosis H37Rv
VO ID	VO_0011175
NCBI Gene ID	885896
NCBI Protein GI	57116926
Locus Tag	Rv1860
Genbank Accession	AL123456
Protein Accession	YP_177849
Taxonomy ID	83332
Gene Starting Position	2107735
Gene Ending Position	2108712
Gene Strand (Orientation)	+
Protein Name	hypothetical protein
Protein pI	4.7
Protein Weight	30636.65
Protein Length	325
Protein Note	Rv1860, (MT1908, MTCY359.0013), len: 325 aa. apa (alternate gene names: mpt32, modD), Ala-, Pro-rich 45/47 kDa secreted protein, very similar to P46842\|N43L_MYCLE from Mycobacterium leprae (287 aa), FASTA scores: opt: 1166, E(): 0, (66.4% identity in 298 aa overlap). Known to be glycosylated fibronectin-binding protein (see some citations). CHANGES IN THE MANNOSYLATION PATTERN OF THIS PROTEIN AFFECT ITS ABILITY TO STIMULATE T-LYMPHOCYTE RESPONSE. MAJOR IMMUNODOMINANT ANTIGEN THAT HAS POTENTIAL AS A VACCINE AGAINST TUBERCULOSIS. APA-ELISA COULD BE USED IN DIAGNOSIS. TBparse score is 0.924.; mpt32; modD
DNA Sequence	>NC_000962.3:2107735-2108712 Mycobacterium tuberculosis H37Rv, complete genome CATGCATCAGGTGGACCCCAACTTGACACGTCGCAAGGGACGATTGGCGGCACTGGCTATCGCGGCGATG GCCAGCGCCAGCCTGGTGACCGTTGCGGTGCCCGCGACCGCCAACGCCGATCCGGAGCCAGCGCCCCCGG TACCCACAACGGCCGCCTCGCCGCCGTCGACCGCTGCAGCGCCACCCGCACCGGCGACACCTGTTGCCCC CCCACCACCGGCCGCCGCCAACACGCCGAATGCCCAGCCGGGCGATCCCAACGCAGCACCTCCGCCGGCC GACCCGAACGCACCGCCGCCACCTGTCATTGCCCCAAACGCACCCCAACCTGTCCGGATCGACAACCCGG TTGGAGGATTCAGCTTCGCGCTGCCTGCTGGCTGGGTGGAGTCTGACGCCGCCCACTTCGACTACGGTTC AGCACTCCTCAGCAAAACCACCGGGGACCCGCCATTTCCCGGACAGCCGCCGCCGGTGGCCAATGACACC CGTATCGTGCTCGGCCGGCTAGACCAAAAGCTTTACGCCAGCGCCGAAGCCACCGACTCCAAGGCCGCGG CCCGGTTGGGCTCGGACATGGGTGAGTTCTATATGCCCTACCCGGGCACCCGGATCAACCAGGAAACCGT CTCGCTCGACGCCAACGGGGTGTCTGGAAGCGCGTCGTATTACGAAGTCAAGTTCAGCGATCCGAGTAAG CCGAACGGCCAGATCTGGACGGGCGTAATCGGCTCGCCCGCGGCGAACGCACCGGACGCCGGGCCCCCTC AGCGCTGGTTTGTGGTATGGCTCGGGACCGCCAACAACCCGGTGGACAAGGGCGCGGCCAAGGCGCTGGC CGAATCGATCCGGCCTTTGGTCGCCCCGCCGCCGGCGCCGGCACCGGCTCCTGCAGAGCCCGCTCCGGCG CCGGCGCCGGCCGGGGAAGTCGCTCCTACCCCGACGACACCGACACCGCAGCGGACCTTACCGGCCTG
Protein Sequence	>YP_177849.1 hypothetical protein Rv1860 [Mycobacterium tuberculosis H37Rv] MHQVDPNLTRRKGRLAALAIAAMASASLVTVAVPATANADPEPAPPVPTTAASPPSTAAAPPAPATPVAP PPPAAANTPNAQPGDPNAAPPPADPNAPPPPVIAPNAPQPVRIDNPVGGFSFALPAGWVESDAAHFDYGS ALLSKTTGDPPFPGQPPPVANDTRIVLGRLDQKLYASAEATDSKAAARLGSDMGEFYMPYPGTRINQETV SLDANGVSGSASYYEVKFSDPSKPNGQIWTGVIGSPAANAPDAGPPQRWFVVWLGTANNPVDKGAAKALA ESIRPLVAPPPAPAPAPAEPAPAPAPAGEVAPTPTTPTPQRTLPA
Molecule Role	Protective antigen
Molecule Role Annotation	We show that the 50- to 55-kDa Apa protein, specified by the Rv1860 gene of M. tuberculosis, can elicit both lymphoproliferative response and gamma interferon (IFN-gamma) production. A poxvirus recombinant expressing the Apa protein conferred a significant level of protective immunity in guinea pigs against a challenge dose of virulent M. tuberculosis (Kumar et al., 2003).
Related Vaccines(s)	M. tuberculosis DNA Vaccine APADNA priming and APAMVA boosting
References

Apa