VIOLIN: Vaccine Investigation and Online Information Network

Ply
Gene Name	Ply
Sequence Strain (Species/Organism)	Streptococcus pneumoniae D39
VO ID	VO_0011192
NCBI Gene ID	4441611
NCBI Protein GI	116515376
Locus Tag	SPD_1726
Genbank Accession	CP000410
Protein Accession	YP_817150
Taxonomy ID	373153
Gene Starting Position	1721456
Gene Ending Position	1722871
Gene Strand (Orientation)	-
Protein Name	pneumolysin
Protein pI	4.91
Protein Weight	48435.22
Protein Length	471
Protein Note	equivalent gene in S.pneumoniae TIGR4 = SP1923; equivalent gene in S.pneumoniae R6 = spr1739; identified by match to protein family HMM PF01289
DNA Sequence	>NC_008533.1:1721456-1722871 Streptococcus pneumoniae D39, complete genome CCTAGTCATTTTCTACCTTATCCTCTACCTGAGGATAGAGAGTTGTTCCCCAAATAGAAATCGTCCGCTT ACGCACTAGTGGCAAATCGGTTTTTTCATAAACCGTACGCCACCATTCCCAGGCAAGCCCGGTACACTCT CTAATTTTGACAGAGAGATTACGAACATTCCCTTTTAAAGGAATACTAGTGGTAAAGTGAGCCGTCAAAT CCTGCCCATTTCTGTCCCAAGCCTTAGGAGTCAAGACTTCCTTACCTTGATGATCATAGGATAATTCATC CCAAGTAATATAATATTGGGCAACATAGGCACCACTATGATCCAGCAGTAAATCTCCGTTTCTGTAAGCT GTAACCTTAGTCTCAACATAGTCTGTACTGTTTTGAAAGGTCGCAACTACATTGTCACGTAAAAAAGAAG TTGTATAGGAAATCGGCAAGCCTGGATGATCTGCTGTAAAGCGACTGCCTTCTTGAATCAAGTCCTCTAC CATATCCACCTTGCCTGTTACAACTCGGGCACCCGAACTTGGGTCGCCCCCTAAAATAACCGCCTTCACT TCTGTATTGTCCAAAATCTGCTTCCACTCTGTCTGAGGAGCTACCTTGACTCCTTTTATCAAAGCTTCAA AAGCAGCCTCTACTTCATCACTCTTACTCGTGGTTTCCAACTTGAGATAGACTTGGCGCCCATAAGCAAC ACTCGAAATATAGACCAAAGGACGCTCTGCAGAAATTCCTCTCTGTTTTAAATCCTCTACCGTTACAGTA TCTTGAAACACATCTCCTGGATTTTTAACAGCGTCTACGCTGACTGTATAATAAATCTGCTTAAAATTAA CAATCTGAATCTGCTTTTCACCTGAATGGACAGAGTTAAAATCAATATCAAGAGAATTCCCTGTCTTTTC AAAGTCAGAACCAAACTTGACCTTGAGTTGTTCCATGCTGTGAGCCGTTATTTTTTCATACTGCATTCTA GCTGGGACATTATTGACCTGACCATAATCTTGATGCCACTTAGCCAACAAATCGTTTACCGCTCCGCGAA CACTTGAATTGCTGGGGTCTTCCACTTGGAGAAAGCTATCGCTACTTGCCAAACCAGGCAAATCAATACT ATAAGTCATCGGAGCACGATCAACCGCAAGAAGAGTGGGATTATTCTCTAACAAGGTCTCATCCACTACG AGAAGTGCTCCAGGATAGAGGCGACTGTCGTTGGTAGCTGTTACAGAAATATCACTTGTATTTGTCGACA AGCTCCGCTTCTTTCTTTCGATAACAACAAACTCATCGGGTAGCTGATTACCCTCTTTGATGAAACGATT TTCAATACTTTCTCCCTGATGGGTCAAGAGTTTCTTTTTATCGTAATTCATAGCTAGTATAAAGTCATTT ACTGCTTTATTTGCCA
Protein Sequence	>YP_817150.1 pneumolysin [Streptococcus pneumoniae D39] MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRL YPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQ VNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNP GDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQ ILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDY VETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRN LSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
Molecule Role	Protective antigen
Molecule Role Annotation	Immunization with the pneumococcal proteins pneumolysin (Ply), choline binding protein A (CbpA), or pneumococcal surface protein A (PspA) elicits protective responses against invasive pneumococcal disease in animal models.(Chen et al., 2015)
Additional Molecule Role	Virmugen
Additional Molecule Role Annotation	The protective effects a pneumolysin mutant (DeltaA146 Ply) against pneumococcal infections in mice was evaluated. Vaccinated mice were challenged with pneumococcal strain D39 at 1,500 times the LD50 (1.5 x 105 CFU) and the vaccinated mice survived significantly longer than control mice after challenge (Wu et al., 2010).
References

Ply