VIOLIN: Vaccine Investigation and Online Information Network

LplA
Gene Name	LplA
Sequence Strain (Species/Organism)	Streptococcus pneumoniae R6
VO ID	VO_0011194
NCBI Gene ID	934072
NCBI Protein GI	15903091
Locus Tag	spr1047
Genbank Accession	AE007317
Protein Accession	NP_358641
Taxonomy ID	171101
Gene Starting Position	1041220
Gene Ending Position	1042209
Gene Strand (Orientation)	-
Protein Name	lipoate-protein ligase
Protein pI	5.54
Protein Weight	35144.06
Protein Length	329
Protein Note	Putative
DNA Sequence	>NC_003098.1:1041220-1042209 Streptococcus pneumoniae R6 chromosome, complete genome TTTATCCAACTACCGCTTCAGCGATTTCTTCACGGCTAATACCAGCGAAGTAGCGTGTGATATCAATGGT TTTTAGCGCCTTAAGAACATCTTCGCGTTCGTATTTCACCCCACGAAGGACATCTTCTACTGCAGCAACG TCTTCAATACCAAAGAAGTCACCATAAATCTTGATGTCTTGGATTTTTGATTCAGTAACGTTAGCAAAGA CTTCAACCTTACCACTAGTGAATTTGGTTCCACGACGGACGTTAAATTCAGGTGATTTACCATAGTTCCA GTCCCAAGTTCCAAACTTAGTATCCTTGATGCGATTGATTTCGGCCAATTCTTCTTCTGAAAAGACGTAT TCAGTCATCTCTGGGTACTCTTTTTTCATGTATTCCAAGAGTAAATCACGGAATTTTTCGACTGTGATTT TTTTTGGTAATTCATTGATAATATTGGTTACACGGGCACGGACGGATTTCACACCTTTTGATTCAAATTT ATCTTTTGAAACCTTAAGGGCATTTGCGAGGACTGACAAATCAACGTCAAAGAGCAAGCAACCGTGGTGC ATGATACGGCCGTTGATATAGGCTTGGGCATTGCCACAGAACTTCTTACCATCAATCTCAAGGTCATTAC GACCTGTGAACTCAGCTTTAACCCCAAGTTGAGCCAGGGTATTGATAACCGGAGTTGAGAAGCTCTTGAA GTCAAATGCCTTATTTTCATCTTCTTTGGAGATGATCGTGTAGTTGAGGTTATTTAAATCGTGGTAAACA GCTCCACCACCACTAATACGGCGAACTACCTCAATACCATTTTCGCGAACATAATCACGGTTGATTTCTT CGATAGTGTTCTGGTGACGACCAACAATGATAGATGGCTTGTTAATCCAAAGTAGGAAGATTTGATCCTC ATCCAAAAGGTGTTTAAAGGCGTATTCTTCCAAGGCAATATTAAAAGCAGTGTCATTTGAATGATTGATA ATGTATTTCA
Protein Sequence	>NP_358641.1 lipoate-protein ligase [Streptococcus pneumoniae R6] MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVGRHQNTIEEINRDYVRENGIEVVRRIS GGGAVYHDLNNLNYTIISKEDENKAFDFKSFSTPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYIN GRIMHHGCLLFDVDLSVLANALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYP EMTEYVFSEEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIYGDFF GIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG
Molecule Role	Protective antigen
Molecule Role Annotation	The production of a recombinant vaccine was achieved following PCR amplification of putative lipoate protein ligase (Lpl), with subsequent cloning and expression of the genes in E. coli. The putative lipoate protein ligase (Lpl) , resulted in a reduced CFU titre and a trend towards reduced mortality in an animal sepsis model (Morsczeck et al., 2008).
References

LplA