VIOLIN: Vaccine Investigation and Online Information Network

P4
Gene Name	P4
Sequence Strain (Species/Organism)	Leishmania amazonensis
VO ID	VO_0011288
NCBI Nucleotide GI	29165286
NCBI Protein GI	29165287
Protein Accession	AAO65599.1
Other Database IDs	CDD:280435 CDD:211382
Taxonomy ID	5659
Gene Strand (Orientation)	?
Protein Name	P4 nuclease
Protein pI	7.27
Protein Weight	32555.78
Protein Length	371
Protein Note	S1/P1 Nuclease; pfam02265
DNA Sequence	>gi\|29165286\|gb\|AY219923.1\| Leishmania amazonensis P4 nuclease mRNA, complete cds ATGCCTGCGCCTGTCAGTCTCCGTCTGCCTCTCACAGCGCTGTGCCTCCTGGTGCTCAGCTCTGCGCTGT GCGTGACTGAGGTGCTCGGATGGGGCTGCGTGGGCCACATGCTCCTCGCCGAGATCGCGCGGCGCCAGCT GGACTTAGAGAATGAGGAAAAGATCGAGCTGATGGCGGCAGTATTTTCGGGCAGCGGTCCGTTCCCGATG TCTCCGAGCATGGTGCAAGCGGCATGCTGGGCGGATGACGTGAAGCTTTGGCGTCAGTACGCCATGAGCA CGTGGCACTTCTACGCCATGCCATATAACCCCGGAAACATCAATATCACCGATCCGGTCAATACAGTGAA CGCCGTCACTGTGTGCCTTGATATGGTGACCTCGCTTAAGAACTCGAAAGCGCCGCTGTACTTGTTGAAC TTTGCTTGGGTGAACCTGGTGCACATCTTTGGCGATCTGCATCAGCCCCTGCACACGATATCTAGGTACA CTACCGCCTACCCGCACGGAGATCAAGGAGGCAACGCGATTTCGGTTCGTGTAGGCGGAAAAAAGGTGAA GCTGCATGCCCTCTGGGATAATATTTGCAGTGCTACGCCACCACGCTACCAGCGGCCCCTCTCCCACACC GACCTCTTCGCCCTCTCTGCGACAGCGGACGGGCTGGTGGAAACTTACACGTTTTCAGAGGCGTTGGAGA CACTCGTGGACGTGATGGCGATTCATGAGGAGAGCTACATGTTTGCGGTAAACACCAGCTACCCCGGCGT CACACCAGGTGGGACTCTCAGTCGTGCCTACCTTGATAAATGCAAGCGTGTCGCTGAGGCTCGACTGACG CTTGGCGGCTACCGCCTCGGTTACCTTCTGAATCAGCTGCTGTCGGGCATCACTGTGGACAAGGCCGCAC TGGAGGCGCACCGCGCTGCGCGTCCGAAGTGGAGCGCGTAA
Protein Sequence	>AAO65599.1 P4 nuclease [Leishmania amazonensis] MPAPVSLRLPLTALCLLVLSSALCVTEVLGWGCVGHMLLAEIARRQLDLENEEKIELMAAVFSGSGPFPM SPSMVQAACWADDVKLWRQYAMSTWHFYAMPYNPGNINITDPVNTVNAVTVCLDMVTSLKNSKAPLYLLN FAWVNLVHIFGDLHQPLHTISRYTTAYPHGDQGGNAISVRVGGKKVKLHALWDNICSATPPRYQRPLSHT DLFALSATADGLVETYTFSEALETLVDVMAIHEESYMFAVNTSYPGVTPGGTLSRAYLDKCKRVAEARLT LGGYRLGYLLNQLLSGITVDKAALEAHRAARPKWSA
Molecule Role	Protective antigen
Molecule Role Annotation	Susceptible BALB/c mice were immunized with the DNA encoding P4 alone, P4/IL-12, or P4/HSP70 prior to challenge with L. amazonensis promastigotes. Mice immunized with P4 alone had a delayed onset of lesion development and significantly smaller lesions than the infection control group at 11 weeks postinfection (P < 0.05). P4/IL-12 immunized animals showed no sign of lesion development in three independent experiments (P < 0.01). Our results indicate that different vaccine combinations, including DNA encoding P4, HSP70, or IL-12, can provide significant protection against both Old World and New World cutaneous leishmaniasis (Campbell et al., 2003).
References

P4