VIOLIN: Vaccine Investigation and Online Information Network

PstS3
Gene Name	PstS3
Sequence Strain (Species/Organism)	Mycobacterium tuberculosis H37Rv
VO ID	VO_0012370
NCBI Gene ID	885366
NCBI Protein GI	57116798
Locus Tag	Rv0928
Genbank Accession	AL123456
Protein Accession	YP_177768
Taxonomy ID	83332
Gene Starting Position	1034902
Gene Ending Position	1036014
Gene Strand (Orientation)	+
Protein Name	phosphate ABC transporter substrate-binding lipoprotein PstS
Protein pI	5.85
Protein Weight	35472.01
Protein Length	370
Protein Note	Rv0928, (MTCY21C12.22), len: 370 aa. pstS3 (previously known as phoS2), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to others from Mycobacterium leprae e.g. Q50099\|PSTS3\|PHOS1 phosphate-binding protein 3 precursor (328 aa), FASTA scores: opt: 1772, E(): 0, (79.6% identity in 328 aa overlap); and highly similar to others e.g. AAF74819.1\|AF137360_1\|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa). Also highly similar to Rv0932c\|MTCY08D9.07\|pstS2 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN (370 aa); and Rv0934\|pstS1 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN (374 aa) from Mycobacterium tuberculosis (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0934c). Contains lipoprotein signature (PS00013) at N-terminus. BELONGS TO FAMILY OF PHOSPHATE RECEPTORS FOR BACTERIAL ABC-TYPE LIPOPROTEIN TRANSPORTERS.; phoS2
DNA Sequence	>NC_000962.3:1034902-1036014 Mycobacterium tuberculosis H37Rv, complete genome ATTGAAACTCAACCGATTTGGTGCCGCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCCGCGTGT GGTAACGACGACAATGTGACCGGGGGAGGTGCAACCACTGGCCAGGCGTCGGCGAAGGTCGATTGCGGGG GGAAGAAGACACTCAAAGCCAGTGGGTCGACGGCGCAGGCCAACGCGATGACCCGCTTTGTCAACGTGTT CGAGCAGGCCTGCCCCGGCCAAACCCTGAACTACACGGCCAATGGTTCGGGCGCTGGAATCAGCGAATTT AATGGCAACCAAACCGATTTCGGTGGCTCAGATGTACCCCTGAGCAAGGACGAGGCCGCAGCGGCGCAGC GGCGTTGCGGCTCGCCGGCGTGGAATCTGCCGGTGGTGTTCGGCCCGATCGCGGTTACCTACAACCTCAA CAGCGTTTCCTCGCTAAATTTGGACGGCCCCACGTTGGCGAAGATCTTCAACGGCTCCATTACGCAGTGG AACAATCCCGCGATCCAGGCGCTGAACCGCGACTTCACGCTGCCAGGTGAGCGGATTCACGTGGTGTTCC GCAGCGATGAGTCGGGGACCACGGACAACTTCCAGAGGTACCTGCAGGCCGCGTCCAACGGTGCGTGGGG TAAGGGCGCTGGAAAGTCGTTCCAAGGCGGCGTCGGTGAGGGCGCGCGGGGTAACGATGGCACGTCAGCG GCCGCGAAGAACACCCCGGGGTCGATCACCTACAACGAGTGGTCGTTCGCCCAGGCGCAGCACCTGACCA TGGCCAACATCGTCACTTCGGCTGGTGGGGACCCGGTGGCGATTACTATCGACTCGGTCGGCCAGACGAT CGCCGGGGCCACCATCTCCGGGGTGGGCAACGACCTGGTGCTCGACACGGACTCGTTCTACCGGCCGAAG CGTCCCGGCTCCTATCCGATCGTGTTAGCGACATACGAAATCGTTTGCTCGAAGTATCCCGACTCGCAGG TTGGCACGGCTGTGAAGGCGTTCCTGCAGAGCACTATCGGCGCCGGTCAAAGCGGCCTGGGGGACAACGG ATACATCCCAATTCCGGACGAGTTCAAATCGAGGCTGTCGACTGCGGTCAACGCGATCGCCTG
Protein Sequence	>YP_177768.1 phosphate ABC transporter substrate-binding lipoprotein PstS [Mycobacterium tuberculosis H37Rv] MKLNRFGAAVGVLAAGALVLSACGNDDNVTGGGATTGQASAKVDCGGKKTLKASGSTAQANAMTRFVNVF EQACPGQTLNYTANGSGAGISEFNGNQTDFGGSDVPLSKDEAAAAQRRCGSPAWNLPVVFGPIAVTYNLN SVSSLNLDGPTLAKIFNGSITQWNNPAIQALNRDFTLPGERIHVVFRSDESGTTDNFQRYLQAASNGAWG KGAGKSFQGGVGEGARGNDGTSAAAKNTPGSITYNEWSFAQAQHLTMANIVTSAGGDPVAITIDSVGQTI AGATISGVGNDLVLDTDSFYRPKRPGSYPIVLATYEIVCSKYPDSQVGTAVKAFLQSTIGAGQSGLGDNG YIPIPDEFKSRLSTAVNAIA
Molecule Role	Protective antigen
Molecule Role Annotation	Mice vaccinated with PstS-3 DNA demonstrated significant and sustained reduction in bacterial CFU numbers in spleen and lungs for 3 mo after M. tuberculosis challenge, as compared with CFU counts in mice vaccinated with control DNA (Tanghe et al., 1999).
References

PstS3