VIOLIN: Vaccine Investigation and Online Information Network

PstS1
Gene Name	PstS1
Sequence Strain (Species/Organism)	Mycobacterium tuberculosis H37Rv
VO ID	VO_0012407
NCBI Gene ID	885724
NCBI Protein GI	57116801
Locus Tag	Rv0934
Genbank Accession	AL123456
Protein Accession	YP_177770
3D structure: PDB ID	1PC3
Taxonomy ID	83332
Gene Starting Position	1042114
Gene Ending Position	1043238
Gene Strand (Orientation)	+
Protein Name	phosphate ABC transporter substrate-binding lipoprotein PstS
Protein pI	5.02
Protein Weight	36356.95
Protein Length	374
Protein Note	Rv0934, (MTCY08D9.05c), len: 374 aa. pstS1 (previously known as phoS1 or phoS), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to Rv0932c\|MTCY08D9.07\|pstS2 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN from Mycobacterium tuberculosis (370 aa), FASTA scores: opt: 460, E(): 5.9e-19, (31.2% identity in 375 aa overlap); and Rv0928\|MTCY21C12.22\|pstS3 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 435, E():1.1e-17, (30.0% identity in 380 aa overlap) (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0928c). Also highly similar to MTCY08D9.05c\|P15712\|PAB_MYCTU PROTEIN ANTIGEN B PRECURSOR from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 2459, E(): 0, (100% identity in 374 aa overlap). Contains a prokaryotic membrane lipoprotein lipid attachment site (PS00013) at the N-terminus so the 23 aa leader peptide sequence is probably removed. BELONGS TO FAMILY OF PHOSPHATE RECEPTORS FOR BACTERIAL ABC-TYPE LIPOPROTEIN TRANSPORTERS.; phoS1; phoS
DNA Sequence	>NC_000962.3:1042114-1043238 Mycobacterium tuberculosis H37Rv, complete genome CGTGAAAATTCGTTTGCATACGCTGTTGGCCGTGTTGACCGCTGCGCCGCTGCTGCTAGCAGCGGCGGGC TGTGGCTCGAAACCACCGAGCGGTTCGCCTGAAACGGGCGCCGGCGCCGGTACTGTCGCGACTACCCCCG CGTCGTCGCCGGTGACGTTGGCGGAGACCGGTAGCACGCTGCTCTACCCGCTGTTCAACCTGTGGGGTCC GGCCTTTCACGAGAGGTATCCGAACGTCACGATCACCGCTCAGGGCACCGGTTCTGGTGCCGGGATCGCG CAGGCCGCCGCCGGGACGGTCAACATTGGGGCCTCCGACGCCTATCTGTCGGAAGGTGATATGGCCGCGC ACAAGGGGCTGATGAACATCGCGCTAGCCATCTCCGCTCAGCAGGTCAACTACAACCTGCCCGGAGTGAG CGAGCACCTCAAGCTGAACGGAAAAGTCCTGGCGGCCATGTACCAGGGCACCATCAAAACCTGGGACGAC CCGCAGATCGCTGCGCTCAACCCCGGCGTGAACCTGCCCGGCACCGCGGTAGTTCCGCTGCACCGCTCCG ACGGGTCCGGTGACACCTTCTTGTTCACCCAGTACCTGTCCAAGCAAGATCCCGAGGGCTGGGGCAAGTC GCCCGGCTTCGGCACCACCGTCGACTTCCCGGCGGTGCCGGGTGCGCTGGGTGAGAACGGCAACGGCGGC ATGGTGACCGGTTGCGCCGAGACACCGGGCTGCGTGGCCTATATCGGCATCAGCTTCCTCGACCAGGCCA GTCAACGGGGACTCGGCGAGGCCCAACTAGGCAATAGCTCTGGCAATTTCTTGTTGCCCGACGCGCAAAG CATTCAGGCCGCGGCGGCTGGCTTCGCATCGAAAACCCCGGCGAACCAGGCGATTTCGATGATCGACGGG CCCGCCCCGGACGGCTACCCGATCATCAACTACGAGTACGCCATCGTCAACAACCGGCAAAAGGACGCCG CCACCGCGCAGACCTTGCAGGCATTTCTGCACTGGGCGATCACCGACGGCAACAAGGCCTCGTTCCTCGA CCAGGTTCATTTCCAGCCGCTGCCGCCCGCGGTGGTGAAGTTGTCTGACGCGTTGATCGCGACGATTTCC AGCTA
Protein Sequence	>YP_177770.1 phosphate ABC transporter substrate-binding lipoprotein PstS [Mycobacterium tuberculosis H37Rv] MKIRLHTLLAVLTAAPLLLAAAGCGSKPPSGSPETGAGAGTVATTPASSPVTLAETGSTLLYPLFNLWGP AFHERYPNVTITAQGTGSGAGIAQAAAGTVNIGASDAYLSEGDMAAHKGLMNIALAISAQQVNYNLPGVS EHLKLNGKVLAAMYQGTIKTWDDPQIAALNPGVNLPGTAVVPLHRSDGSGDTFLFTQYLSKQDPEGWGKS PGFGTTVDFPAVPGALGENGNGGMVTGCAETPGCVAYIGISFLDQASQRGLGEAQLGNSSGNFLLPDAQS IQAAAAGFASKTPANQAISMIDGPAPDGYPIINYEYAIVNNRQKDAATAQTLQAFLHWAITDGNKASFLD QVHFQPLPPAVVKLSDALIATISS
Molecule Role	Protective antigen
Molecule Role Annotation	Vaccination with the recombinant PstS-1 antigen and cholera toxin significantly protected against the challenge given 10 days later, but only marginally after 12 weeks (Falero-Diaz et al., 2000).
References

PstS1