VIOLIN: Vaccine Investigation and Online Information Network

Chlamydophila pneumoniae

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. CopN (Protective antigen)
2. FabD (Protective antigen)
3. LcrE (Protective antigen)
4. PknD (Protective antigen)
5. Ssb (Protective antigen)
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

83558

2. Disease:

Pneumonia

3. Introduction

Chlamydophila pneumoniae is a species of Chlamydophila bacteria[1][2][3] that infects humans and is a major cause of pneumonia. C. pneumoniae has a complex life cycle and must infect another cell in order to reproduce and thus is classified as an obligate intracellular pathogen. This atypical bacterium commonly causes pharyngitis, bronchitis and atypical pneumonia mainly in elderly and debilitated patients but in healthy adults also. C. pneumoniae infection has been implicated in several chronic lung diseases by serology and direct antigen detection. Acute lower respiratory tract infection caused by C. pneumoniae seems often to precede attacks of asthma in both children and adults but is also involved in some exacerbations of chronic bronchitis. More importantly it seems to be strongly associated with chronic obstructive lung disease irrespective of exacerbation status. Moreover, persistently elevated C. pneumoniae antibody titers have been observed in sarcoidosis and lung cancer (Wiki: Chlamydophila pneumoniae).

II. Vaccine Related Pathogen Genes

1. CopN

Gene Name : CopN
Sequence Strain (Speices/Organism) : Chlamydophila pneumoniae
VO ID : VO_0010884
NCBI Protein GI : 33241669
Other Database IDs : CDD:164083
Taxonomy ID : 182082
Gene Strand (Orientation) : ?
Protein Name : CopN
Protein pI : 4.72
Protein Weight : 41321.12
Protein Length : 399
Protein Note : type strain

Protein Sequence : Show Sequence

>gi|33241669|ref|NP_876610.1| CopN [Chlamydophila pneumoniae TW-183]
MAASGGTGGLGGTQGVNLAAVEAAAAKADAAEVVASQEGSEMNMIQQSQDLTNPAAATRTKKKEEKFQTL
ESRKKGEAGKAEKKSESTEEKPDTDLADKYASGNSEISGQELRGLRDAIGDDASPEDILALVQEKIKDPA
LQSTALDYLVQTTPPSQGKLKEALIQARNTHTEQFGRTAIGAKNILFASQEYADQLNVSPSGLRSLYLEV
TGDTHTCDQLLSMLQDRYTYQDMAIVSSFLMKGMATELKRQGPYVPSAQLQVLMTETRNLQAVLTSYDYF
ESRVPILLDSLKAEGIQTPSDLNFVKVAESYHKIINDKFPTASKVEREVRNLIGDDVDSVTGVLNLFFSA
LRQTSSRLFSSADKRQQLGAMIANALDAVNINNEDYPKASDFPKPYPWS

Molecule Role : Protective antigen
Molecule Role Annotation : Results of this study showed that intranasal immunization of BALB/c mice with heat-aggregated CopN protein and an Escherichia coli heat-labile toxin (LT) induced a strong immune response. The immunization induced statistically significant protection against intranasal C. pneumoniae challenge, the level of which correlated with the magnitude of CopN-specific lymphocyte proliferation (Tammiruusu et al., 2007).
Related Vaccine(s): C. pneumoniae CopN protein vaccine

2. FabD

Gene Name : FabD
Sequence Strain (Speices/Organism) : Chlamydophila pneumoniae CWL029
VO ID : VO_0010895
NCBI Gene ID : 894994
NCBI Protein GI : 15618217
Locus Tag : CPn0297
Genbank Accession : AE001363
Protein Accession : NP_224502
Taxonomy ID : 115713
Gene Starting Position : 334770
Gene Ending Position : 335696
Gene Strand (Orientation) : -
Protein Name : acyl-carrier-protein S-malonyltransferase
Protein pI : 4.92
Protein Weight : 31463.85
Protein Length : 308

DNA Sequence : Show Sequence

>gi|15617929:334770-335696 Chlamydophila pneumoniae CWL029, complete genome
ATCATACCTCTGATAGGAATTTTTCAATCTGAGCAAAAGTACCAAGACTTGTAATCGGTTTAGAAATCCC
TATAGAGCGATTTAAACCAGCCAAAACTTTTCCTGGACCTAATTCTAAAAACTCATCCACCTCTGATTCG
ATATGGTAACAACTCTGATACCATAACGTAGGTGATGTCATTTGCCGAGCTAAACACTCTCGCATTTCTT
CAGTATTTACTAAAGATTTTCCTACCACGTGTGACACTAAGGGAAGGCTAGAATCTTTCATGCATAAAGC
ATAAATGTCTGGAGCTAAGCCATCTTGAGCAACTTGCATTAAAGGAGTATGAAATGCTCCAGACACCTTT
AAACGAACTGCTTTTTTACATCCTAAATCACGAAATAACTCAATCGCTTGGTCTACTTTTTCTGCTATTC
CAGCCACTACAAGCTGTTTGGGTGCATTATAATTAGCAATCCAAATTCCTTGACCAAGACTTGTTATATT
TTCCTCTATAACTTCAGAGGGAAGCCCTAATAAAGCCGCCATAGCCCCTGGGCTCTGATTACAAGCTTCA
TTCATTAACTGACCACGCTTTCTAACAAGCTCAAGGCCGTCGAGCACGGAGATTCTATCGGAAGCAACTA
AAGCAGTATACTCCCCTAAACTTAATCCAGAGACTAAAGAAGGCTGAATAGAAGAACGCTGAGATAGAAC
CTTTACCACAGCCATGCTATGAAGATAAATAGCTAGCTGACTATGTACTGTTTCCATCAAAAGATCCTCA
GGACCTTCAAACATAATTGAAGTCAGAGAAAATCCTAACCTTTCATTAGCAAAATCAAAAAGCTCTCTAA
CCTCAGGATACTCCATATATAGGTCTTGTCCCATACCTACATATTGGCTCCCTTGTCCTGGGAACAAAAA
AGCATAACGTTTTTTCA

Protein Sequence : Show Sequence

>gi|15618217|ref|NP_224502.1| acyl-carrier-protein S-malonyltransferase [Chlamydophila pneumoniae CWL029]
MKKRYAFLFPGQGSQYVGMGQDLYMEYPEVRELFDFANERLGFSLTSIMFEGPEDLLMETVHSQLAIYLH
SMAVVKVLSQRSSIQPSLVSGLSLGEYTALVASDRISVLDGLELVRKRGQLMNEACNQSPGAMAALLGLP
SEVIEENITSLGQGIWIANYNAPKQLVVAGIAEKVDQAIELFRDLGCKKAVRLKVSGAFHTPLMQVAQDG
LAPDIYALCMKDSSLPLVSHVVGKSLVNTEEMRECLARQMTSPTLWYQSCYHIESEVDEFLELGPGKVLA
GLNRSIGISKPITSLGTFAQIEKFLSEV

Molecule Role : Protective antigen
Molecule Role Annotation : M-ID vaccination with fabD generated a response that resulted in moderately, but significantly reduced total C. pneumoniae lung loads as compared to control mice vaccinated with a plasmid expressing a non-Chlamydia ORF (p ≤ 0.019). This resulted in the ability of fabD to mediate a moderate, but statistically significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).
Related Vaccine(s): C. pneumoniae DNA vaccine encoding FabD

3. LcrE

Gene Name : LcrE
Sequence Strain (Speices/Organism) : Chlamydophila pneumoniae CWL029
VO ID : VO_0010883
NCBI Gene ID : 895078
NCBI Protein GI : 15618244
Locus Tag : CPn0324
Genbank Accession : AE001363
Protein Accession : NP_224529
Taxonomy ID : 115713
Gene Starting Position : 369491
Gene Ending Position : 370690
Gene Strand (Orientation) : +
Protein Name : low calcium response E
Protein pI : 4.72
Protein Weight : 41321.12
Protein Length : 399

DNA Sequence : Show Sequence

>gi|15617929:369491-370690 Chlamydophila pneumoniae CWL029, complete genome
TATGGCAGCATCAGGAGGCACAGGTGGTTTAGGAGGCACTCAGGGTGTCAACCTTGCAGCTGTAGAAGCT
GCAGCTGCAAAAGCAGATGCAGCAGAAGTTGTAGCCAGCCAAGAAGGTTCTGAGATGAACATGATTCAAC
AATCTCAGGACCTGACAAATCCCGCAGCAGCAACACGCACGAAAAAAAAGGAAGAGAAGTTTCAAACTCT
AGAATCTCGGAAAAAAGGAGAAGCTGGAAAGGCTGAGAAAAAATCTGAATCTACAGAAGAGAAGCCTGAC
ACAGATCTTGCTGATAAGTATGCTTCTGGGAATTCTGAAATCTCTGGTCAAGAACTTCGCGGCCTGCGTG
ATGCAATAGGAGACGATGCTTCTCCAGAAGACATTCTTGCTCTTGTACAAGAGAAAATTAAAGACCCAGC
TCTGCAATCCACAGCTTTGGACTACCTGGTTCAAACGACTCCACCCTCCCAAGGTAAATTAAAAGAAGCG
CTTATCCAAGCAAGGAATACTCATACGGAGCAATTCGGACGAACTGCTATTGGTGCGAAAAACATCTTAT
TTGCCTCTCAAGAATATGCAGACCAACTGAATGTTTCTCCTTCAGGGCTTCGCTCTTTGTACTTAGAAGT
GACTGGAGACACACATACCTGTGATCAGCTACTTTCTATGCTTCAAGACCGCTATACCTACCAAGATATG
GCTATTGTCAGCTCCTTTCTAATGAAAGGAATGGCAACAGAATTAAAAAGGCAGGGTCCCTACGTACCCA
GTGCGCAACTACAAGTTCTCATGACAGAAACTCGTAACCTGCAAGCAGTTCTTACCTCGTACGATTACTT
TGAAAGTCGCGTTCCTATTTTACTCGATAGCTTAAAAGCTGAGGGAATCCAAACTCCTTCTGATCTAAAC
TTTGTGAAGGTAGCTGAGTCCTACCATAAAATCATTAACGATAAGTTCCCAACAGCATCTAAAGTAGAAC
GAGAAGTCCGCAATCTCATAGGAGACGATGTTGATTCTGTGACCGGTGTCTTGAACTTATTCTTTTCTGC
TTTACGTCAAACGTCGTCACGCCTTTTCTCTTCAGCAGACAAACGTCAGCAATTAGGAGCTATGATTGCT
AATGCTTTAGATGCTGTAAATATAAACAATGAAGATTATCCCAAAGCATCAGACTTCCCTAAACCCTATC
CTTGGTCATG

Protein Sequence : Show Sequence

>gi|15618244|ref|NP_224529.1| low calcium response E [Chlamydophila pneumoniae CWL029]
MAASGGTGGLGGTQGVNLAAVEAAAAKADAAEVVASQEGSEMNMIQQSQDLTNPAAATRTKKKEEKFQTL
ESRKKGEAGKAEKKSESTEEKPDTDLADKYASGNSEISGQELRGLRDAIGDDASPEDILALVQEKIKDPA
LQSTALDYLVQTTPPSQGKLKEALIQARNTHTEQFGRTAIGAKNILFASQEYADQLNVSPSGLRSLYLEV
TGDTHTCDQLLSMLQDRYTYQDMAIVSSFLMKGMATELKRQGPYVPSAQLQVLMTETRNLQAVLTSYDYF
ESRVPILLDSLKAEGIQTPSDLNFVKVAESYHKIINDKFPTASKVEREVRNLIGDDVDSVTGVLNLFFSA
LRQTSSRLFSSADKRQQLGAMIANALDAVNINNEDYPKASDFPKPYPWS

Molecule Role : Protective antigen
Molecule Role Annotation : The immunogenicity and protective effect of recombinant LcrE protein combined either with Freund's or Alum adjuvant were investigated in mice. The immunization with both protocols resulted in a significant reduction of the number of viable C. pneumoniae in the lungs after challenge. Results confirm that LcrE induces protective immunity in mice (Faludi et al., 2009).
Related Vaccine(s): C. pneumoniae LcrE protein vaccine

4. PknD

Gene Name : PknD
Sequence Strain (Speices/Organism) : Chlamydophila pneumoniae CWL029
VO ID : VO_0010894
NCBI Gene ID : 895704
NCBI Protein GI : 161353778
Locus Tag : CPn0095
Genbank Accession : AE001363
Protein Accession : NP_224303
Taxonomy ID : 115713
Gene Starting Position : 115994
Gene Ending Position : 118792
Gene Strand (Orientation) : +
Protein Name : serine/threonine-protein kinase
Protein pI : 6.03
Protein Weight : 102446.37
Protein Length : 932
Protein Note : PknD; responsible for phosphorylation of proteins on serine and threonine residues; similar to eukaryotic Ser/Thr kinases; in Chlamydia trachomatis itseems to interact with Pkn1, another serine/threonine-protein kinase

DNA Sequence : Show Sequence

>gi|15617929:115994-118792 Chlamydophila pneumoniae CWL029, complete genome
TTTGGAGCGCTATGATATTGTTAGAATTATTGGAAAGGGAGGCATGGGTGAAGTCTATCTTGCCTACGAT
CCTGTATGTTCTCGTAAAGTAGCTCTTAAAAAAATTCGTGAAGATCTTGCAGAAAATCCTCTTTTGAAAA
GGAGGTTTTTACGAGAGGCAAGAATTGCCGCTGACCTTATTCATCCTGGTGTTGTTCCTGTCTATACTAT
TTACAGCGAGAAAGATCCTGTATACTACACGATGCCCTACATAGAGGGATATACACTAAAAACCTTACTG
AAGAGTGTATGGCAAAAGGAATCCCTGTCTAAGGAATTAGCAGAGAAAACTTCTGTAGGGGCATTTCTTT
CTATCTTTCATAAGATCTGCTGCACTATAGAATATGTCCATTCTCGGGGCATTCTTCATCGCGACCTTAA
ACCCGATAACATCTTATTAGGTCTTTTTAGTGAGGCTGTAATCTTAGATTGGGGAGCAGCAGTTGCCTGT
GGAGAAGAAGAGGATCTTCTTGATATAGATGTCAGCAAAGAGGAGGTGCTCTCTTCAAGAATGACAATTC
CAGGAAGAATAGTAGGGACTCCAGATTATATGGCTCCTGAGAGGCTCCTGGGCCATCCAGCTTCTAAAAG
TACAGACATTTATGCTTTAGGAGTGGTTCTTTATCAGATGCTCACTCTCTCTTTTCCTTATAGAAGAAAA
AAAGGAAAGAAAATAGTTCTTGACGGTCAGAGAATTCCAAGTCCTCAAGAGGTAGCTCCTTATCGAGAAA
TCCCTCCGTTTCTTTCCGCTGTAGTGATGAGAATGTTGGCTGTAGATCCTCAAGAGCGCTATTCTTCGGT
AACAGAGCTTAAGGAAGATATCGAGAGTCATCTGAAAGGGAGTCCTAAATGGACTTTAACCACAGCCCTG
CCACCTAAAAAATCTTCTAGTTGGAAGCTAAACGAACCTATTTTACTTTCTAAGTATTTTCCAATGTTGG
AGGTCTCTCCAGCGTCATGGTACAGTTTAGCAATCTCTAATATTGAGAGTTTTTCTGAGATGCGCTTGGA
GTATACTCTTTCTAAAAAAGGCTTGAACGAAGGCTTTGGTATTTTACTTCCCACGTCAGAAAATGCTTTA
GGGGGAGATTTTTACCAGGGGTATGGCTTTTGGCTGCATATTAAGGAGAGAACCTTATCCGTGTCTCTGG
TGAAAAATAGCCTAGAAATCCAGAGGTGCTCTCAAGATTTGGAATCTGATAAAGAGACCTTCTTGATAGC
TTTAGAGCAGCATAATCATAGTTTATCTTTGTTTGTCGATGGTACGACTTGGCTTATCCATATGAATTAT
CTGCCAAGTCGTAGTGGGCGAGTCGCTATCATAGTTCGCGATATGGAAGATATCCTGGAAGATATAGGCA
TTTTTGAAAGTAGTGGCTCTTTGAGGGTCAGTTGTCTTGCTGTTCCTGACGCTTTTCTTGCTGAGAAGTT
ATATGATCGCGCTTTAGTGCTTTACCGAAGGATCGCAGAATCTTTCCCAGGACGTAAAGAAGGTTATGAA
GCAAGGTTCAGAGCAGGAATTACAGTTTTAGAGAAGGCCTCTACAGATAATAATGAACAGGAATTTGCTC
TAGCCATTGAAGAATTCTCAAAATTACATGACGGGGTTGCTGCTCCCTTAGAATACCTTGGTAAGGCTTT
AGTATATCAGAGACTCCAAGAGTATAATGAAGAAATTAAGAGTTTGCTATTAGCATTGAAACGTTATTCG
CAGCATCCTGAAATCTTTAGGCTTAAAGACCATGTGGTTTACCGACTCCATGAGAGCTTTTATAAACGGG
ATCGCCTTGCTCTGGTGTTCATGATTTTAGTATTGGAAATAGCTCCCCAGGCAATCACTCCAGGGCAGGA
AGAAAAAATCCTGGTTTGGTTAAAGGACAAATCTCGGGCTACCTTATTTTGCCTCCTGGATCCCACGGTC
TTAGAGCTGCGCTCTTCTAAAATGGAATTATTTTTAAGTTATTGGTCTGGGTTTATTCCCCATCTCAATA
GTCTATTTCATAGAGCTTGGGATCAAAGCGATGTGCGAGCTTTGATCGAGATTTTCTATGTTGCTTGTGA
TCTTCATAAATGGCAGTTTCTCTCTTCTTGTATCGACATATTTAAAGAGTCTCTTGAGGATCAGAAAGCC
ACAGAAGAGATTGTTGAGTTCTCTTTCGAGGATTTAGGGGCATTTCTTTTTGCTATTCAGAGCATCTTTA
ACAAGGAAGATGCAGAGAAGATCTTTGTTTCTAATGATCAATTATCGCCAATCCTTCTTGTTTATATATT
CGATCTTTTTGCAAATCGTGCTCTTCTGGAATCTCAAGGAGAGGCTATTTTTCAGGCTTTGGATCTCATC
CGAAGTAAAGTTCCTGAAAATTTTTATCATGATTACTTGCGGAATCATGAAATCCGAGCGCATCTTTGGT
GCCGCAATGAGAAGGCTCTAAGCACGATTTTTGAAAACTATACAGAGAAACAGCTAAAGGATGAGCAACA
TGAACTGTTCGTTCTCTATGGATGTTACCTTGCTCTTATACAAGGTGCTGAGGCGGCAAAGCAGCATTTT
GATGTATGTCGTGAAGATCGCATTTTCCCTGCTTCATTATTAGCTAGAAATTACAATCGTTTAGGTCTTC
CCAAAGATGCTCTTAGCTATCAAGAGCGGCGTTTGTTATTGCGACAAAAGTTTCTCTATTTCCATTGTCT
TGGTAACCACGACGAGCGTGACTTATGCCAGACTATGTATCACCTCTTAACCGAAGAATTTCAGCTTTA

Protein Sequence : Show Sequence

>gi|161353778|ref|NP_224303.2| serine/threonine-protein kinase [Chlamydophila pneumoniae CWL029]
MERYDIVRIIGKGGMGEVYLAYDPVCSRKVALKKIREDLAENPLLKRRFLREARIAADLIHPGVVPVYTI
YSEKDPVYYTMPYIEGYTLKTLLKSVWQKESLSKELAEKTSVGAFLSIFHKICCTIEYVHSRGILHRDLK
PDNILLGLFSEAVILDWGAAVACGEEEDLLDIDVSKEEVLSSRMTIPGRIVGTPDYMAPERLLGHPASKS
TDIYALGVVLYQMLTLSFPYRRKKGKKIVLDGQRIPSPQEVAPYREIPPFLSAVVMRMLAVDPQERYSSV
TELKEDIESHLKGSPKWTLTTALPPKKSSSWKLNEPILLSKYFPMLEVSPASWYSLAISNIESFSEMRLE
YTLSKKGLNEGFGILLPTSENALGGDFYQGYGFWLHIKERTLSVSLVKNSLEIQRCSQDLESDKETFLIA
LEQHNHSLSLFVDGTTWLIHMNYLPSRSGRVAIIVRDMEDILEDIGIFESSGSLRVSCLAVPDAFLAEKL
YDRALVLYRRIAESFPGRKEGYEARFRAGITVLEKASTDNNEQEFALAIEEFSKLHDGVAAPLEYLGKAL
VYQRLQEYNEEIKSLLLALKRYSQHPEIFRLKDHVVYRLHESFYKRDRLALVFMILVLEIAPQAITPGQE
EKILVWLKDKSRATLFCLLDPTVLELRSSKMELFLSYWSGFIPHLNSLFHRAWDQSDVRALIEIFYVACD
LHKWQFLSSCIDIFKESLEDQKATEEIVEFSFEDLGAFLFAIQSIFNKEDAEKIFVSNDQLSPILLVYIF
DLFANRALLESQGEAIFQALDLIRSKVPENFYHDYLRNHEIRAHLWCRNEKALSTIFENYTEKQLKDEQH
ELFVLYGCYLALIQGAEAAKQHFDVCREDRIFPASLLARNYNRLGLPKDALSYQERRLLLRQKFLYFHCL
GNHDERDLCQTMYHLLTEEFQL

Molecule Role : Protective antigen
Molecule Role Annotation : IM-ID vaccination with CPn0095 (pknD) generated a response that resulted in moderately, but significantly reduced total C. pneumoniae lung loads as compared to control mice vaccinated with a plasmid expressing a non-Chlamydia ORF (p ≤ 0.019). This resulted in the ability of CPN0095 to mediate a moderate, but statistically significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).
Related Vaccine(s): C. pneumoniae DNA vaccine encoding PknD

5. Ssb

Gene Name : Ssb
Sequence Strain (Speices/Organism) : Chlamydophila pneumoniae CWL029
VO ID : VO_0010896
NCBI Gene ID : 894901
NCBI Protein GI : 15618301
Locus Tag : CPn0386
Genbank Accession : AE001363
Protein Accession : NP_224586
Taxonomy ID : 115713
Gene Starting Position : 434042
Gene Ending Position : 434524
Gene Strand (Orientation) : -
Protein Name : single-stranded DNA-binding protein
Protein pI : 5.29
Protein Weight : 16434.22
Protein Length : 160
Protein Note : binds to single stranded DNA and may facilitate the binding and interaction of other proteins to DNA

DNA Sequence : Show Sequence

>gi|15617929:434042-434524 Chlamydophila pneumoniae CWL029, complete genome
ATTAAAAAGGAACATCTTCACAGACATACTGCTGTTCTTGACCATAACCAGCATACATATCTTTATCTTT
AATAGCTTCTGCGTCCAGTGCTTCACCTTCAAACCCTACGGATACAGATTCATATCCCACTTGCTGATGA
TTGTCTTCTAAAGATGGAGAACGGCTGCCTTCATTGCGACCGAAAGGACTGAATTTCAAAGAATCTACAC
TAATCACTAAAGAAGATTGCGGTGAACCATCTTTGCTCATGTAACTCTCTACAGAGATATCGCCAGCAAC
AATGACTCCTGAGCCTTTCTTCAAGTAAGGAAGCATCTTATCATAGCGATTGTGCCAAATATTGCATTTG
CACCAAACAGTTTCATCTTTCATTCCAACTCGAGTCTTCACTCCCAGTCTCAGAGTGATCACACGTTTTC
CTTTGGAAGTCATTCGCTCTTCAGGATCTGCTCCAAGGTAACCAGCAAAATGCCCAAACATCA

Protein Sequence : Show Sequence

>gi|15618301|ref|NP_224586.1| single-stranded DNA-binding protein [Chlamydophila pneumoniae CWL029]
MMFGHFAGYLGADPEERMTSKGKRVITLRLGVKTRVGMKDETVWCKCNIWHNRYDKMLPYLKKGSGVIVA
GDISVESYMSKDGSPQSSLVISVDSLKFSPFGRNEGSRSPSLEDNHQQVGYESVSVGFEGEALDAEAIKD
KDMYAGYGQEQQYVCEDVPF

Molecule Role : Protective antigen
Molecule Role Annotation : Mice vaccinated with candidate gene ssb showed significant reduction of spleen chlamydial loads as compared to naïve, non-protected control mice (p ≤ 0.048). This resulted in the ability of ssb to mediate a modest, but significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).
Related Vaccine(s): C. pneumoniae DNA vaccine encoding Ssb

III. Vaccine Information

1. C. pneumoniae CopN protein vaccine

a. Vaccine Ontology ID:

VO_0011432

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

C. pneumoniae copN

e. Gene Engineering of CopN

Type: Recombinant protein preparation
Description: C. pneumoniae CopN (gene lcrE; position 0324 of C. pneumoniae CWL029), was produced in a Bacillus subtilis protein expression system as a soluble protein. Recombinant CopN protein was dissolved in PBS at a concentration of 1 mg/ml and heated to 100 °C for 10 min after which the visible precipitation of protein was discernible. C. pneumoniae preparation was boiled for 10 min in a water bath at a concentration of 2.5 × 10⁷ IFU/ml in SPG. E. coli heat-labile toxin, LT (kindly provided by Prof. G. Dougan, Imperial Collage, London, UK) was added to heat-aggregated protein suspension to a final concentration of 12.5 μg/ml (Tammiruusu et al., 2007).
Detailed Gene Information: Click here.

f. Adjuvant:

Escherichia coli heat-labile toxin (LT)

g. Immunization Route

Intranasal

h. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: Mice were immunized intranasally with 40 μg of heat-aggregated CopN/ 40 μl dose or 106 heat-treated C. pneumoniae inclusion forming unit (IFU) (approximately 1 μg of protein)/40 μl dose. Mice immunized intranasally with disrupted HL cells (Mock) or PBS were used as control. Fourteen days after the first immunization, the mice were boosted once with the same dose of antigen. All immunizations were performed under methoxyflurane anaesthesia (Metofane, Pitman-Moore, Mundelein, IL, USA) (Tammiruusu et al., 2007).
Challenge Protocol: At 14 days after the second immunization, the mice were challenged intranasally with 10⁵ IFU of C. pneumoniae in 40 μl of SPG under Metofane anaesthesia. At certain time points after infection, three to six mice were sacrificed, lungs were mechanically homogenized in SPG and dilutions of lung supernatant were cultured on HL cell monolayers (Tammiruusu et al., 2007).
Efficacy: Intranasal immunization of BALB/c mice with heat-aggregated CopN protein and an Escherichia coli heat-labile toxin (LT) induced a strong immune response. The immunization induced statistically significant protection against intranasal C. pneumoniae challenge, the level of which correlated with the magnitude of CopN-specific lymphocyte proliferation (Tammiruusu et al., 2007).

2. C. pneumoniae DNA vaccine encoding FabD

a. Vaccine Ontology ID:

VO_0011434

b. Type:

DNA vaccine

c. Status:

Research

d. Antigen

C. pneumoniae fabD

e. Gene Engineering of FabD

Type: DNA vaccine construction
Description: The genome sequence of C. pneumoniae isolate CDC/CWL-029 (ATCC strain VR-1310) was extracted from Genbank (AE001363, 1,230,230 bp). The 1052 annotated genes of C. pneumoniae were imported into a gene-splitting and primer prediction program; primer pairs to amplify 1263 ORFs of 1.5 kb or less were exported. A 1.5 kb maximum ORF length was chosen to ensure sufficient PCR quality and yields, and this generated a few additional fragments (Li et al., 2006).
Detailed Gene Information: Click here.

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Host Strain: A/J
Vaccination Protocol: For intranasal inoculation, mice received a light isoflurane inhalation anesthesia. Vaccine protection control mice were inoculated with a low dose of 5 × 10⁶ C. pneumoniae elementary bodies in 30 μl SPG buffer (Li et al., 2006).
Challenge Protocol: High-dose challenge infection was performed 4 weeks after the last gene gun genetic vaccination or low dose inoculation of live C. pneumoniae, and 6 weeks after the last intramuscular-intradermal genetic vaccination, by intranasal inoculation of 1 × 10⁸ C. pneumoniae elementary bodies in 30 μl SPG buffer. Mice were sacrificed by CO2 inhalation 2 h, 3 days, 10 days, or 15 days after inoculation, and lungs and spleen were weighed, snap frozen in liquid nitrogen, and stored at −80 °C until further processing (Li et al., 2006).
Efficacy: M-ID vaccination with fabD generated a response that resulted in moderately, but significantly reduced total C. pneumoniae lung loads as compared to control mice vaccinated with a plasmid expressing a non-Chlamydia ORF (p ≤ 0.019). This resulted in the ability of fabD to mediate a moderate, but statistically significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).

3. C. pneumoniae DNA vaccine encoding PknD

a. Vaccine Ontology ID:

VO_0011424

b. Type:

DNA vaccine

c. Status:

Research

d. Antigen

C. pneumoniae serine/threonine-protein kinase, PknD

e. Gene Engineering of PknD

Type: DNA vaccine construction
Description: The genome sequence of C. pneumoniae isolate CDC/CWL-029 (ATCC strain VR-1310) was extracted from Genbank (AE001363, 1,230,230 bp). The 1052 annotated genes of C. pneumoniae were imported into a gene-splitting and primer prediction program; primer pairs to amplify 1263 ORFs of 1.5 kb or less were exported. A 1.5 kb maximum ORF length was chosen to ensure sufficient PCR quality and yields, and this generated a few additional fragments (Li et al., 2006).
Detailed Gene Information: Click here.

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Host Strain: A/J
Vaccination Protocol: For intranasal inoculation, mice received a light isoflurane inhalation anesthesia. Vaccine protection control mice were inoculated with a low dose of 5 × 10⁶ C. pneumoniae elementary bodies in 30 μl SPG buffer (Li et al., 2006).
Challenge Protocol: High-dose challenge infection was performed 4 weeks after the last gene gun genetic vaccination or low dose inoculation of live C. pneumoniae, and 6 weeks after the last intramuscular-intradermal genetic vaccination, by intranasal inoculation of 1 × 10⁸ C. pneumoniae elementary bodies in 30 μl SPG buffer. Mice were sacrificed by CO2 inhalation 2 h, 3 days, 10 days, or 15 days after inoculation, and lungs and spleen were weighed, snap frozen in liquid nitrogen, and stored at −80 °C until further processing (Li et al., 2006).
Efficacy: IM-ID vaccination with CPn0095 (pknD) generated a response that resulted in moderately, but significantly reduced total C. pneumoniae lung loads as compared to control mice vaccinated with a plasmid expressing a non-Chlamydia ORF (p ≤ 0.019). This resulted in the ability of CPN0095 to mediate a moderate, but statistically significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).

4. C. pneumoniae DNA vaccine encoding Ssb

a. Vaccine Ontology ID:

VO_0011425

b. Type:

DNA vaccine

c. Status:

Research

d. Antigen

C. pneumoniae single-stranded DNA-binding protein, ssb

e. Gene Engineering of Ssb

Type: DNA vaccine construction
Description: The genome sequence of C. pneumoniae isolate CDC/CWL-029 (ATCC strain VR-1310) was extracted from Genbank (AE001363, 1,230,230 bp). The 1052 annotated genes of C. pneumoniae were imported into a gene-splitting and primer prediction program; primer pairs to amplify 1263 ORFs of 1.5 kb or less were exported. A 1.5 kb maximum ORF length was chosen to ensure sufficient PCR quality and yields, and this generated a few additional fragments (Li et al., 2006).
Detailed Gene Information: Click here.

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Host Strain: A/J
Vaccination Protocol: For intranasal inoculation, mice received a light isoflurane inhalation anesthesia. Vaccine protection control mice were inoculated with a low dose of 5 × 10⁶ C. pneumoniae elementary bodies in 30 μl SPG buffer (Li et al., 2006).
Challenge Protocol: High-dose challenge infection was performed 4 weeks after the last gene gun genetic vaccination or low dose inoculation of live C. pneumoniae, and 6 weeks after the last intramuscular-intradermal genetic vaccination, by intranasal inoculation of 1 × 10⁸ C. pneumoniae elementary bodies in 30 μl SPG buffer. Mice were sacrificed by CO2 inhalation 2 h, 3 days, 10 days, or 15 days after inoculation, and lungs and spleen were weighed, snap frozen in liquid nitrogen, and stored at −80 °C until further processing (Li et al., 2006).
Efficacy: Mice vaccinated with candidate gene ssb showed significant reduction of spleen chlamydial loads as compared to naïve, non-protected control mice (p ≤ 0.048). This resulted in the ability of ssb to mediate a modest, but significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).

5. C. pneumoniae LcrE protein vaccine

a. Vaccine Ontology ID:

VO_0011435

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

C. pneumoniae LcrE

e. Gene Engineering of LcrE

Type: Recombinant protein preparation
Description: A 1218-kb DNA fragment containing the lcrE gene (GenBank ID 15618244, Locus tag CPn0324) was amplified by PCR, using C. pneumoniae (CWL029 ATCC) DNA as template. The PCR was performed in a GeneAmp II (Applied Biosystems, Foster City, CA, USA) thermocycler with Advantage GC cDNA polymerase (Clontech, Mountain View, CA, USA), and the amplification conditions were set as recommended by the manufacturer. The amplicon was digested with NdeI and BamHI and inserted into p6HisF-11d (icl) pET vector by digesting it with the same enzymes and replacing the icl gene (Faludi et al., 2009).
Detailed Gene Information: Click here.

f. Adjuvant:

Either Freund's or Alum adjuvants

g. Immunization Route

Subcutaneous injection

h. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: The mice in groups of 25 were immunized subcutaneously into the tail base with the purified LcrE protein diluted in phosphate buffered saline (PBS) at a dose of 20 μg mixed with 25 μl Alum (Aluminum hydroxide Gel, Sigma) or 75 μl Freund's adjuvants (Chemicon International, Temecula, CA, USA; 1st inoculation with complete and 2nd and 3rd inoculations with incomplete Freund's adjuvant) in 0.15-ml volume 3 times at 3-week intervals (Faludi et al., 2009).
Challenge Protocol: Two weeks after the last immunization, the immunized and non-immunized mice (absolute naive animals) were challenged with 4×10⁵ inclusion forming unit (IFU) C. pneumoniae (CWL029, ATCC) in 25 μl PBS intranasally under pentobarbital sodium anesthesia (Faludi et al., 2009).
Efficacy: The immunogenicity and protective effect of recombinant LcrE protein combined either with Freund's or Alum adjuvant were investigated in mice. The immunization with both protocols resulted in a significant reduction of the number of viable C. pneumoniae in the lungs after challenge. Results confirm that LcrE induces protective immunity in mice (Faludi et al., 2009).

IV. References

1. Faludi et al., 2009: Faludi I, Burian K, Csanadi A, Miczak A, Lu X, Kakkar VV, Gonczol E, Endresz V. Adjuvant modulation of the immune response of mice against the LcrE protein of Chlamydophila pneumoniae. International journal of medical microbiology : IJMM. 2009; 299(7); 520-528. [PubMed: 19451031].

2. Li et al., 2006: Li D, Borovkov A, Vaglenov A, Wang C, Kim T, Gao D, Sykes KF, Kaltenboeck B. Mouse model of respiratory Chlamydia pneumoniae infection for a genomic screen of subunit vaccine candidates. Vaccine. 2006; 24(15); 2917-2927. [PubMed: 16434129].

3. Penttilä et al., 2000: Penttilä T, Vuola JM, Puurula V, Anttila M, Sarvas M, Rautonen N, Mäkelä PH, Puolakkainen M. Immunity to Chlamydia pneumoniae induced by vaccination with DNA vectors expressing a cytoplasmic protein (Hsp60) or outer membrane proteins (MOMP and Omp2). Vaccine. 2000; 19(9-10); 1256-1265. [PubMed: 11137265].

4. Tammiruusu et al., 2007: Tammiruusu A, Penttilä T, Lahesmaa R, Sarvas M, Puolakkainen M, Vuola JM. Intranasal administration of chlamydial outer protein N (CopN) induces protection against pulmonary Chlamydia pneumoniae infection in a mouse model. Vaccine. 2007; 25(2); 283-290. [PubMed: 16949182].

5. Wiki: Chlamydophila pneumoniae: Chlamydophila pneumoniae [http://en.wikipedia.org/wiki/Chlamydophila_pneumoniae]