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Chlamydophila pneumoniae

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Disease
    3. Introduction
    4. Microbial Pathogenesis
    5. Host Ranges and Animal Models
  2. Vaccine Related Pathogen Genes
    1. copN (Protective antigen)
    2. copN (Protective antigen)
    3. enolase (Protective antigen)
    4. FabD (Protective antigen)
    5. groEL_3 (Protective antigen)
    6. groES (Protective antigen)
    7. LcrE (Protective antigen)
    8. lcrE (Protective antigen)
    9. major outer membrane protein, MOMP (Protective antigen)
    10. OmpH-like outer membrane protein (Protective antigen)
    11. PknD (Protective antigen)
    12. pmp10 (Protective antigen)
    13. pmp2 (Protective antigen)
    14. Ssb (Protective antigen)
  3. Vaccine Related Host Genes
    1. Ifng (Interferon gamma)
    2. IgA
  4. Vaccine Information
    1. C. pneumoniae CopN protein vaccine
    2. C. pneumoniae DNA vaccine encoding FabD
    3. C. pneumoniae DNA vaccine encoding PknD
    4. C. pneumoniae DNA vaccine encoding Ssb
    5. C. pneumoniae DNA vaccine pHSP-60
    6. C. pneumoniae LcrE protein vaccine
  5. References
I. General Information
1. NCBI Taxonomy ID:
83558
2. Disease:
Pneumonia
3. Introduction
Chlamydophila pneumoniae is a species of Chlamydophila bacteria[1][2][3] that infects humans and is a major cause of pneumonia. C. pneumoniae has a complex life cycle and must infect another cell in order to reproduce and thus is classified as an obligate intracellular pathogen. This atypical bacterium commonly causes pharyngitis, bronchitis and atypical pneumonia mainly in elderly and debilitated patients but in healthy adults also. C. pneumoniae infection has been implicated in several chronic lung diseases by serology and direct antigen detection. Acute lower respiratory tract infection caused by C. pneumoniae seems often to precede attacks of asthma in both children and adults but is also involved in some exacerbations of chronic bronchitis. More importantly it seems to be strongly associated with chronic obstructive lung disease irrespective of exacerbation status. Moreover, persistently elevated C. pneumoniae antibody titers have been observed in sarcoidosis and lung cancer (Wiki: Chlamydophila pneumoniae).
4. Microbial Pathogenesis
Chlamydophila pneumoniae is a small bacterium (0.2 to 1 micrometer) that undergoes several transformations during its life cycle. It exists as an elementary body (EB) in between hosts. The EB is not biologically active but is resistant to environmental stresses and can survive outside of a host for a limited time. The EB travels from an infected person to the lungs of a non-infected person in small droplets and is responsible for infection. Once in the lungs, the EB is taken up by cells in a pouch called an endosome by a process called phagocytosis. However, the EB is not destroyed by fusion with lysosomes as is typical for phagocytosed material. Instead, it transforms into a reticulate body and begins to replicate within the endosome. The reticulate bodies must utilize some of the host's cellular machinery to complete its replication. The reticulate bodies then convert back to elementary bodies and are released back into the lung, often after causing the death of the host cell. The EBs are thereafter able to infect new cells, either in the same organism or in a new host. Thus, the life cycle of C. pneumoniae is divided between the elementary body, which is able to infect new hosts but can not replicate, and the reticulate body ,which replicates but is not able to cause new infection (Wiki: Chlamydophila pneumoniae).
5. Host Ranges and Animal Models
In addition to infecting humans, C. pneumoniae also infects and causes disease in Koalas, emerald tree boa (Corallus caninus), iguanas, chameleons, frogs, and turtles (Wiki: Chlamydophila pneumoniae).
1. copN
  • Gene Name : copN
  • Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
  • VO ID : VO_0010884
  • NCBI Protein GI : 33241669
  • Other Database IDs : CDD:274206
  • Taxonomy ID : 182082
  • Gene Strand (Orientation) : ?
  • Protein Name : CopN
  • Protein pI : 4.72
  • Protein Weight : 42139.89
  • Protein Length : 458
  • Protein Note : type strain
  • Protein Sequence : Show Sequence
    >NP_876610.1 CopN [Chlamydophila pneumoniae TW-183]
    MAASGGTGGLGGTQGVNLAAVEAAAAKADAAEVVASQEGSEMNMIQQSQDLTNPAAATRTKKKEEKFQTL
    ESRKKGEAGKAEKKSESTEEKPDTDLADKYASGNSEISGQELRGLRDAIGDDASPEDILALVQEKIKDPA
    LQSTALDYLVQTTPPSQGKLKEALIQARNTHTEQFGRTAIGAKNILFASQEYADQLNVSPSGLRSLYLEV
    TGDTHTCDQLLSMLQDRYTYQDMAIVSSFLMKGMATELKRQGPYVPSAQLQVLMTETRNLQAVLTSYDYF
    ESRVPILLDSLKAEGIQTPSDLNFVKVAESYHKIINDKFPTASKVEREVRNLIGDDVDSVTGVLNLFFSA
    LRQTSSRLFSSADKRQQLGAMIANALDAVNINNEDYPKASDFPKPYPWS
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Results of this study showed that intranasal immunization of BALB/c mice with heat-aggregated CopN protein and an Escherichia coli heat-labile toxin (LT) induced a strong immune response. The immunization induced statistically significant protection against intranasal C. pneumoniae challenge, the level of which correlated with the magnitude of CopN-specific lymphocyte proliferation (Tammiruusu et al., 2007).
  • Related Vaccine(s): C. pneumoniae CopN protein vaccine
2. copN
  • Gene Name : copN
  • Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
  • NCBI Protein GI : AAP98267
  • Other Database IDs : CDD:274206
  • Taxonomy ID : 182082
  • Protein Name : CopN
  • Protein pI : 4.72
  • Protein Weight : 42167.95
  • Protein Length : 453
  • Protein Note : type strain of Chlamydophila pneumoniae;
    biovar: TWAR
  • Protein Sequence : Show Sequence
    >AAP98267.1 CopN [Chlamydia pneumoniae TW-183]
    MAASGGTGGLGGTQGVNLAAVEAAAAKADAAEVVASQEGSEMNMIQQSQDLTNPAAATRTKKKEEKFQTL
    ESRKKGEAGKAEKKSESTEEKPDTDLADKYASGNSEISGQELRGLRDAIGDDASPEDILALVQEKIKDPA
    LQSTALDYLVQTTPPSQGKLKEALIQARNTHTEQFGRTAIGAKNILFASQEYADQLNVSPSGLRSLYLEV
    TGDTHTCDQLLSMLQDRYTYQDMAIVSSFLMKGMATELKRQGPYVPSAQLQVLMTETRNLQAVLTSYDYF
    ESRVPILLDSLKAEGIQTPSDLNFVKVAESYHKIINDKFPTASKVEREVRNLIGDDVDSVTGVLNLFFSA
    LRQTSSRLFSSADKRQQLGAMIANALDAVNINNEDYPKASDFPKPYPWS
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Tammiruusu et al., 2007)
3. enolase
  • Gene Name : enolase
  • Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
  • NCBI Protein GI : AAP98758
  • Other Database IDs : CDD:213580
    CDD:239429
  • Taxonomy ID : 182082
  • Protein Name : enolase
  • Protein pI : 4.37
  • Protein Weight : 44335.42
  • Protein Length : 486
  • Protein Note : type strain of Chlamydophila pneumoniae;
    biovar: TWAR
  • Protein Sequence : Show Sequence
    >AAP98758.1 enolase [Chlamydia pneumoniae TW-183]
    MFEAVIADIQAREILDSRGYPTLHVKVTTSTGSVGEARVPSGASTGKKEALEFRDTDSPRYQGKGVLQAV
    KNVKEILFPLVKGCSVYEQSLIDSLMMDSDGSPNKETLGANAILGVSLATAHAAAATLRRPLYRYLGGCF
    ACSLPCPMMNLINGGMHADNGLEFQEFMIRPIGASSIKEAVNMGADVFHTLKKLLHERGLSTGVGDEGGF
    APNLASNEEALELLLLAIEKAGFTPGKDISLALDCAASSFYNVKTGTYDGRHYEEQIAILSNLCDRYPID
    SIEDGLAEEDYDGWALLTEVLGEKVQIVGDDLFVTNPELILEGISNGLANSVLIKPNQIGTLTETVYAIK
    LAQMAGYTTIISHRSGETTDTTIADLAVAFNAGQIKTGSLSRSERVAKYNRLMEIEEELGSEAIFTDSNV
    FAYEDSEE
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Finco et al., 2005)
4. FabD
  • Gene Name : FabD
  • Sequence Strain (Species/Organism) : Chlamydophila pneumoniae CWL029
  • VO ID : VO_0010895
  • NCBI Gene ID : 894994
  • NCBI Protein GI : 15618217
  • Locus Tag : CPn0297
  • Genbank Accession : AE001363
  • Protein Accession : NP_224502
  • Taxonomy ID : 115713
  • Gene Starting Position : 334770
  • Gene Ending Position : 335696
  • Gene Strand (Orientation) : -
  • Protein Name : malonyl CoA-acyl carrier protein transacylase
  • Protein pI : 4.92
  • Protein Weight : 31463.85
  • Protein Length : 308
  • DNA Sequence : Show Sequence
    >NC_000922.1:334770-335696 Chlamydophila pneumoniae CWL029 chromosome, complete genome
    ATCATACCTCTGATAGGAATTTTTCAATCTGAGCAAAAGTACCAAGACTTGTAATCGGTTTAGAAATCCC
    TATAGAGCGATTTAAACCAGCCAAAACTTTTCCTGGACCTAATTCTAAAAACTCATCCACCTCTGATTCG
    ATATGGTAACAACTCTGATACCATAACGTAGGTGATGTCATTTGCCGAGCTAAACACTCTCGCATTTCTT
    CAGTATTTACTAAAGATTTTCCTACCACGTGTGACACTAAGGGAAGGCTAGAATCTTTCATGCATAAAGC
    ATAAATGTCTGGAGCTAAGCCATCTTGAGCAACTTGCATTAAAGGAGTATGAAATGCTCCAGACACCTTT
    AAACGAACTGCTTTTTTACATCCTAAATCACGAAATAACTCAATCGCTTGGTCTACTTTTTCTGCTATTC
    CAGCCACTACAAGCTGTTTGGGTGCATTATAATTAGCAATCCAAATTCCTTGACCAAGACTTGTTATATT
    TTCCTCTATAACTTCAGAGGGAAGCCCTAATAAAGCCGCCATAGCCCCTGGGCTCTGATTACAAGCTTCA
    TTCATTAACTGACCACGCTTTCTAACAAGCTCAAGGCCGTCGAGCACGGAGATTCTATCGGAAGCAACTA
    AAGCAGTATACTCCCCTAAACTTAATCCAGAGACTAAAGAAGGCTGAATAGAAGAACGCTGAGATAGAAC
    CTTTACCACAGCCATGCTATGAAGATAAATAGCTAGCTGACTATGTACTGTTTCCATCAAAAGATCCTCA
    GGACCTTCAAACATAATTGAAGTCAGAGAAAATCCTAACCTTTCATTAGCAAAATCAAAAAGCTCTCTAA
    CCTCAGGATACTCCATATATAGGTCTTGTCCCATACCTACATATTGGCTCCCTTGTCCTGGGAACAAAAA
    AGCATAACGTTTTTTCA
    
    
  • Protein Sequence : Show Sequence
    >NP_224502.1 malonyl CoA-acyl carrier protein transacylase [Chlamydia pneumoniae CWL029]
    MKKRYAFLFPGQGSQYVGMGQDLYMEYPEVRELFDFANERLGFSLTSIMFEGPEDLLMETVHSQLAIYLH
    SMAVVKVLSQRSSIQPSLVSGLSLGEYTALVASDRISVLDGLELVRKRGQLMNEACNQSPGAMAALLGLP
    SEVIEENITSLGQGIWIANYNAPKQLVVAGIAEKVDQAIELFRDLGCKKAVRLKVSGAFHTPLMQVAQDG
    LAPDIYALCMKDSSLPLVSHVVGKSLVNTEEMRECLARQMTSPTLWYQSCYHIESEVDEFLELGPGKVLA
    GLNRSIGISKPITSLGTFAQIEKFLSEV
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : M-ID vaccination with fabD generated a response that resulted in moderately, but significantly reduced total C. pneumoniae lung loads as compared to control mice vaccinated with a plasmid expressing a non-Chlamydia ORF (p ≤ 0.019). This resulted in the ability of fabD to mediate a moderate, but statistically significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).
  • Related Vaccine(s): C. pneumoniae DNA vaccine encoding FabD
5. groEL_3
  • Gene Name : groEL_3
  • Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
  • NCBI Protein GI : BAA99106
  • Other Database IDs : CDD:223535
    CDD:295468
  • Taxonomy ID : 138677
  • Protein Name : heat shock protein-60
  • Protein pI : 5.11
  • Protein Weight : 54512.45
  • Protein Length : 590
  • Protein Note : synonym:Chlamydia pneumoniae (strain J138)
  • Protein Sequence : Show Sequence
    >BAA99106.1 heat shock protein-60 [Chlamydia pneumoniae J138]
    MSEQEKLSNYNADKKLFSGIDKLFQIVKGSYGPKQSLSPTSFFKERGFYAISQTELSNSYENLGVDFAKA
    MVNKIHKEHSDGATTGLILLHAILQESYAALEKGISTHKLIASLKLQGEKLQEALQQQSWPIKDALKVRN
    IIFSSLHMPTIADHFYNAFSVVGPEGLISITKERENDKTSMDVFQGFKIPAGYASTYFVSDTASRLTRIA
    HPLILITDRKISMIHSLLPLLQEISEQNQHLIIFCEDIDPDVLATLVVNKLQGLLQVTVVTIPQLSTTNQ
    ELAEDIALFTGTHICPCQEASHVLAPEMVTLGSCLSIEISESQTTLIGGLHIPEVLTLKTRQLAEEIRTT
    SCLETKKRLIKSTNRLQSSVAILPTDEDNEPLYTLALKIMESALSRGYVPGGGVALFYASLTLGTPKDDA
    DENSIAISLLQKACCAPLKLLATNADLDGDAVIAKLSSLGTTSLGISVFSREIEDLIAGGILDSLATTST
    ILAQALDTAILVLSSKILILENQYEISTL
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Lu et al., 2012)
6. groES
  • Gene Name : groES
  • Sequence Strain (Species/Organism) : Chlamydia pneumoniae strain TWAR
  • NCBI Nucleotide GI : 40888719
  • NCBI Protein GI : 144501
  • Protein Accession : AAA23125.1
  • Other Database IDs : CDD:238197
    CDD:178988
    GOA:P31682
    UniProtKB/UniProt: P31682
  • Taxonomy ID : 83558
  • Gene Strand (Orientation) : ?
  • Protein Name : putative GroES protein
  • Protein pI : 4.32
  • Protein Weight : 11211.01
  • Protein Length : 163
  • Protein Note : oligomerisation interface [polypeptide binding]
  • DNA Sequence : Show Sequence
    >gi|40888719|gb|M69217.2|CHTGRO Chlamydophila pneumoniae groE operon, complete sequence
    AATTTCACACAGGAAACAGCTATGACCATGATTACGAATCTACGGAGGTGTTGTAACCTCGGATTCCCTA
    TCTGCATTAGAATGGGCTAGAATCCCTCACTTCTACTATAATTTCTATGTTTATCAATATGCGACAGGTA
    TCATAGCTGCCCTCTCCTTTGCTGAAAAAATTCTTACACAAGAACCAGGGCTCTCGAACTTTATTTAAAA
    TTTTTTGAAAAGCGGAAGGTCTGACTTCCCACTCAATATATTAAAGAAATCGGGATTGGATATGACCACC
    TCTGCCTCCATTAGATAAAGCCTTTGCATTCATTACGAAAAAGATAGACCTACTCTCTTCCTTGCTTTCA
    GAAGATTGAGTTTTTAGCACTTAAAATTATTGAGTGCTAAAATTATTGCACAAAAAAAAACGCTTTGTTA
    TCGTGATTGCAGAAATAGCAAAGTCTCTTAGAACGTAAAACATAAGGAGCATATAAATGTCTGATCAAGC
    AACGACCCTCCGAATTAAACCTTTGGGCGATAGAATCTTGGTAAAAAGGGAAGAAGAAGAAGCCACTGCT
    CGTGGAGGAATCATCTTACCCGATACAGCAAAAAAGAAACAAGATCGTGCTGAGGTCCTTGTTTTAGGCA
    CAGGCAAACGAACTGATGACGGTACTCTACTTCCTTTCGAAGTTCAAGTTGGCGATATCATTTTAATGGA
    TAAGTATGCAGGTCAAGAAATCACAATCGATGACGAAGAGTATGTCATTCTACAGTCCAGTGAAATCATG
    GCCGTCCTAAAATAAAATACTAGTTTGCAGATTATAGAAAGTTAAGGAGAACAACGATGGCAGCGAAAAA
    TATTAAATATAATGAAGAAGCCAGAAAAAAAATACATAAAGGGGTAAAAACTCTTGCAGAAGCAGTAAAA
    GTTACTCTAGGTCCTAAAGGACGTCACGTAGTTATAGATAAGAGCTTTGGCTCTCCCCAAGTGACTAAAG
    ATGGTGTTACTGTAGCTAAAGAAATCGAGCTCGAAGACAAACATGAAAACATGGGCGCTCAGATGGTAAA
    AGAAGTCGCCAGCAAAACTGCTGACAAAGCAGGCGACGGAACTACAACAGCAACTGTTCTTGCAGAAGCA
    ATCTATAGCGAAGGTCTAAGAAATGTCACTGCCGGTGCCAATCCTATGGACCTAAAAAGAGGTATCGACA
    AAGCCGTAAAAGTTGTTGTTGATGAACTCAAAAAAATTAGTAAACCTGTACAACATCACAAAGAAATCGC
    TCAAGTAGCTACTATCTCAGCAAATAATGATTCCGAAATCGGAAATCTTATTGCAGAAGCTATGGAAAAA
    GTTGGTAAAAACGGATCCATTACTGTTGAAGAAGCTAAAGGCTTCGAAACTGTTCTCGACGTTGTAGAAG
    GAATGAACTTCAACCGTGGATACCTCTCCAGCTACTTCTCCACAAATCCAGAAACTCAAGAATGCGTTTT
    AGAAGACGCTCTGATTCTAATCTACGATAAAAAAATCTCTGGAATTAAAGACTTCCTTCCAGTTTTACAA
    CAAGTAGCAGAATCTGGACGCCCTCTTTTAATCATTGCAGAAGAAATTGAAGGAGAAGCTTTAGCAACTC
    TAGTAGTCAATAGACTCCGTGCAGGATTCAGAGTCTGTGCAGTGAAAGCTCCTGGTTTCGGTGACAGAAG
    AAAAGCTATGTTAGAAGACATCGCTATCCTTACTGGTGGCCAACTAGTTAGCGAAGAACTTGGCATGAAA
    CTAGAGAATACAACTCTAGCAATGTTAGGAAAAGCTAAGAAAGTTATCGTAACTAAAGAAGATACCACAA
    TCGTCGAAGGCTTAGGAAACAAACCTGATATCCAAGCTCGATGCGACAATATTAAAAAACAAATCGAAGA
    TAGCACTTCAGATTACGACAAAGAAAAACTCCAAGAGCGTTTAGCTAAACTCTCCGGTGGTGTCGCCGTA
    ATCCGCGTAGGAGCTGCTACCGAAATAGAGATGAAAGAGAAAAAAGACAGAGTAGATGATGCACAACACG
    CAACCATTGCAGCTGTCGAAGAAGGAATCCTCCCTGGTGGTGGAACTGCCTTAGTTCGCTGTATCCCTAC
    ACTAGAAGCTTTCCTTCCTATGCTAGCAAACGAAGACGAAGCTATTGGTACTCGTATTATTCTAAAAGCA
    TTAACAGCTCCATTAAAGCAAATTGCAAGTAACGCAGGTAAAGAAGGCGCTATCATTTGTCAGCAAGTTC
    TAGCAAGATCTGCAAATGAAGGCTATGATGCTTTACGTGACGCTTATACAGATATGATTGACGCAGGAAT
    TTTAGATCCAACTAAAGTGACTCGCTCAGCTCTAGAAAGCGCACGTTCTATCGCAGGATTACTCCTCACA
    ACAGAAGCCTTAATCGCTGATATCCCAGAAGAGAAATCTTCTTCAGCTCCAGCGATGCCAAGCGCAGGAA
    TGGACTACTAGTCTCTTAAGCTAGTATTAACAATTTTCCATGAGGTCTCTTTTCTAACTAAAGAAAAGAG
    ACCTTTTTCTTTGGGAATATTCATTTCTTAATCTATCTTAATTATTAAGATATAAAACTCCTCTGCCTAT
    ATGTTTAAACTGCTAAAAAATCTATTTCTTATAGGGTGCTGCATCGTTGGATATTTCTGGATGCGCAAAG
    AAAGTATCGTTGAGCAATGGCTATCTAACCGCCT
  • Protein Sequence : Show Sequence
    >AAA23125.1 putative GroES protein [Chlamydia pneumoniae]
    MSDQATTLRIKPLGDRILVKREEEEATARGGIILPDTAKKKQDRAEVLVLGTGKRTDDGTLLPFEVQVGD
    IILMDKYAGQEITIDDEEYVILQSSEIMAVLK
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): C. pneumoniae DNA vaccine pHSP-60
7. LcrE
  • Gene Name : LcrE
  • Sequence Strain (Species/Organism) : Chlamydophila pneumoniae CWL029
  • VO ID : VO_0010883
  • NCBI Gene ID : 895078
  • NCBI Protein GI : 15618244
  • Locus Tag : CPn0324
  • Genbank Accession : AE001363
  • Protein Accession : NP_224529
  • Taxonomy ID : 115713
  • Gene Starting Position : 369491
  • Gene Ending Position : 370690
  • Gene Strand (Orientation) : +
  • Protein Name : low calcium response protein E
  • Protein pI : 4.72
  • Protein Weight : 41321.12
  • Protein Length : 399
  • DNA Sequence : Show Sequence
    >NC_000922.1:369491-370690 Chlamydophila pneumoniae CWL029 chromosome, complete genome
    TATGGCAGCATCAGGAGGCACAGGTGGTTTAGGAGGCACTCAGGGTGTCAACCTTGCAGCTGTAGAAGCT
    GCAGCTGCAAAAGCAGATGCAGCAGAAGTTGTAGCCAGCCAAGAAGGTTCTGAGATGAACATGATTCAAC
    AATCTCAGGACCTGACAAATCCCGCAGCAGCAACACGCACGAAAAAAAAGGAAGAGAAGTTTCAAACTCT
    AGAATCTCGGAAAAAAGGAGAAGCTGGAAAGGCTGAGAAAAAATCTGAATCTACAGAAGAGAAGCCTGAC
    ACAGATCTTGCTGATAAGTATGCTTCTGGGAATTCTGAAATCTCTGGTCAAGAACTTCGCGGCCTGCGTG
    ATGCAATAGGAGACGATGCTTCTCCAGAAGACATTCTTGCTCTTGTACAAGAGAAAATTAAAGACCCAGC
    TCTGCAATCCACAGCTTTGGACTACCTGGTTCAAACGACTCCACCCTCCCAAGGTAAATTAAAAGAAGCG
    CTTATCCAAGCAAGGAATACTCATACGGAGCAATTCGGACGAACTGCTATTGGTGCGAAAAACATCTTAT
    TTGCCTCTCAAGAATATGCAGACCAACTGAATGTTTCTCCTTCAGGGCTTCGCTCTTTGTACTTAGAAGT
    GACTGGAGACACACATACCTGTGATCAGCTACTTTCTATGCTTCAAGACCGCTATACCTACCAAGATATG
    GCTATTGTCAGCTCCTTTCTAATGAAAGGAATGGCAACAGAATTAAAAAGGCAGGGTCCCTACGTACCCA
    GTGCGCAACTACAAGTTCTCATGACAGAAACTCGTAACCTGCAAGCAGTTCTTACCTCGTACGATTACTT
    TGAAAGTCGCGTTCCTATTTTACTCGATAGCTTAAAAGCTGAGGGAATCCAAACTCCTTCTGATCTAAAC
    TTTGTGAAGGTAGCTGAGTCCTACCATAAAATCATTAACGATAAGTTCCCAACAGCATCTAAAGTAGAAC
    GAGAAGTCCGCAATCTCATAGGAGACGATGTTGATTCTGTGACCGGTGTCTTGAACTTATTCTTTTCTGC
    TTTACGTCAAACGTCGTCACGCCTTTTCTCTTCAGCAGACAAACGTCAGCAATTAGGAGCTATGATTGCT
    AATGCTTTAGATGCTGTAAATATAAACAATGAAGATTATCCCAAAGCATCAGACTTCCCTAAACCCTATC
    CTTGGTCATG
    
    
  • Protein Sequence : Show Sequence
    >NP_224529.1 low calcium response protein E [Chlamydia pneumoniae CWL029]
    MAASGGTGGLGGTQGVNLAAVEAAAAKADAAEVVASQEGSEMNMIQQSQDLTNPAAATRTKKKEEKFQTL
    ESRKKGEAGKAEKKSESTEEKPDTDLADKYASGNSEISGQELRGLRDAIGDDASPEDILALVQEKIKDPA
    LQSTALDYLVQTTPPSQGKLKEALIQARNTHTEQFGRTAIGAKNILFASQEYADQLNVSPSGLRSLYLEV
    TGDTHTCDQLLSMLQDRYTYQDMAIVSSFLMKGMATELKRQGPYVPSAQLQVLMTETRNLQAVLTSYDYF
    ESRVPILLDSLKAEGIQTPSDLNFVKVAESYHKIINDKFPTASKVEREVRNLIGDDVDSVTGVLNLFFSA
    LRQTSSRLFSSADKRQQLGAMIANALDAVNINNEDYPKASDFPKPYPWS
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : The immunogenicity and protective effect of recombinant LcrE protein combined either with Freund's or Alum adjuvant were investigated in mice. The immunization with both protocols resulted in a significant reduction of the number of viable C. pneumoniae in the lungs after challenge. Results confirm that LcrE induces protective immunity in mice (Faludi et al., 2009).
  • Related Vaccine(s): C. pneumoniae LcrE protein vaccine
8. lcrE
  • Gene Name : lcrE
  • Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
  • NCBI Protein GI : NP_224529
  • Other Database IDs : CDD:274206
  • Taxonomy ID : 115713
  • Protein Name : low calcium response protein E
  • Protein pI : 4.68
  • Protein Weight : 42166.97
  • Protein Length : 480
  • Protein Note : type III secretion regulator YopN/LcrE/InvE/MxiC; TIGR02568
  • Protein Sequence : Show Sequence
    >NP_224529.1 low calcium response protein E [Chlamydia pneumoniae CWL029]
    MAASGGTGGLGGTQGVNLAAVEAAAAKADAAEVVASQEGSEMNMIQQSQDLTNPAAATRTKKKEEKFQTL
    ESRKKGEAGKAEKKSESTEEKPDTDLADKYASGNSEISGQELRGLRDAIGDDASPEDILALVQEKIKDPA
    LQSTALDYLVQTTPPSQGKLKEALIQARNTHTEQFGRTAIGAKNILFASQEYADQLNVSPSGLRSLYLEV
    TGDTHTCDQLLSMLQDRYTYQDMAIVSSFLMKGMATELKRQGPYVPSAQLQVLMTETRNLQAVLTSYDYF
    ESRVPILLDSLKAEGIQTPSDLNFVKVAESYHKIINDKFPTASKVEREVRNLIGDDVDSVTGVLNLFFSA
    LRQTSSRLFSSADKRQQLGAMIANALDAVNINNEDYPKASDFPKPYPWS
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation :
9. major outer membrane protein, MOMP
  • Gene Name : major outer membrane protein, MOMP
  • Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
  • NCBI Protein GI : AAB24363
  • Other Database IDs : CDD:279628
  • Taxonomy ID : 83558
  • Protein Name : major outer membrane protein
  • Protein pI : 9.6
  • Protein Weight : 6184.55
  • Protein Length : 131
  • Protein Note : MOMP
  • Protein Sequence : Show Sequence
    >AAB24363.1 major outer membrane protein, partial [Chlamydia pneumoniae]
    DNIRIAQPKLPTAVLNLTAWNPSLLGNATALSTTDSFSDFMQIVSCQINKFKSRKA
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Atanu et al., 2013)
10. OmpH-like outer membrane protein
  • Gene Name : OmpH-like outer membrane protein
  • Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
  • NCBI Protein GI : AAP98243
  • Other Database IDs : CDD:225381
  • Taxonomy ID : 182082
  • Protein Name : OmpH-like outer membrane protein
  • Protein pI : 4.77
  • Protein Weight : 19553.64
  • Protein Length : 250
  • Protein Note : type strain of Chlamydophila pneumoniae;
    biovar: TWAR
  • Protein Sequence : Show Sequence
    >AAP98243.1 OmpH-like outer membrane protein [Chlamydia pneumoniae TW-183]
    MKKLLFSTFLLVLGSTSAAHANLGYVNLKRCLEESDLGKKETEELEAMKQQFVKNAEKIEEELTSIYNKL
    QDEDYMESLSDSASEELRKKFEDLSGEYNAYQSQYYQSINQSNVKRIQKLIQEVKIAAESVRSKEKLEAI
    LNEEAVLAIAPGTDKTTEIIAILNESFKKQN
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Finco et al., 2005)
11. PknD
  • Gene Name : PknD
  • Sequence Strain (Species/Organism) : Chlamydophila pneumoniae CWL029
  • VO ID : VO_0010894
  • NCBI Gene ID : 895704
  • NCBI Protein GI : 161353778
  • Locus Tag : CPn0095
  • Genbank Accession : AE001363
  • Protein Accession : NP_224303
  • Taxonomy ID : 115713
  • Gene Starting Position : 115994
  • Gene Ending Position : 118792
  • Gene Strand (Orientation) : +
  • Protein Name : serine/threonine protein kinase
  • Protein pI : 6.03
  • Protein Weight : 102446.37
  • Protein Length : 932
  • Protein Note : PknD; responsible for phosphorylation of proteins on serine and threonine residues; similar to eukaryotic Ser/Thr kinases; in Chlamydia trachomatis itseems to interact with Pkn1, another serine/threonine-protein kinase
  • DNA Sequence : Show Sequence
    >NC_000922.1:115994-118792 Chlamydophila pneumoniae CWL029 chromosome, complete genome
    TTTGGAGCGCTATGATATTGTTAGAATTATTGGAAAGGGAGGCATGGGTGAAGTCTATCTTGCCTACGAT
    CCTGTATGTTCTCGTAAAGTAGCTCTTAAAAAAATTCGTGAAGATCTTGCAGAAAATCCTCTTTTGAAAA
    GGAGGTTTTTACGAGAGGCAAGAATTGCCGCTGACCTTATTCATCCTGGTGTTGTTCCTGTCTATACTAT
    TTACAGCGAGAAAGATCCTGTATACTACACGATGCCCTACATAGAGGGATATACACTAAAAACCTTACTG
    AAGAGTGTATGGCAAAAGGAATCCCTGTCTAAGGAATTAGCAGAGAAAACTTCTGTAGGGGCATTTCTTT
    CTATCTTTCATAAGATCTGCTGCACTATAGAATATGTCCATTCTCGGGGCATTCTTCATCGCGACCTTAA
    ACCCGATAACATCTTATTAGGTCTTTTTAGTGAGGCTGTAATCTTAGATTGGGGAGCAGCAGTTGCCTGT
    GGAGAAGAAGAGGATCTTCTTGATATAGATGTCAGCAAAGAGGAGGTGCTCTCTTCAAGAATGACAATTC
    CAGGAAGAATAGTAGGGACTCCAGATTATATGGCTCCTGAGAGGCTCCTGGGCCATCCAGCTTCTAAAAG
    TACAGACATTTATGCTTTAGGAGTGGTTCTTTATCAGATGCTCACTCTCTCTTTTCCTTATAGAAGAAAA
    AAAGGAAAGAAAATAGTTCTTGACGGTCAGAGAATTCCAAGTCCTCAAGAGGTAGCTCCTTATCGAGAAA
    TCCCTCCGTTTCTTTCCGCTGTAGTGATGAGAATGTTGGCTGTAGATCCTCAAGAGCGCTATTCTTCGGT
    AACAGAGCTTAAGGAAGATATCGAGAGTCATCTGAAAGGGAGTCCTAAATGGACTTTAACCACAGCCCTG
    CCACCTAAAAAATCTTCTAGTTGGAAGCTAAACGAACCTATTTTACTTTCTAAGTATTTTCCAATGTTGG
    AGGTCTCTCCAGCGTCATGGTACAGTTTAGCAATCTCTAATATTGAGAGTTTTTCTGAGATGCGCTTGGA
    GTATACTCTTTCTAAAAAAGGCTTGAACGAAGGCTTTGGTATTTTACTTCCCACGTCAGAAAATGCTTTA
    GGGGGAGATTTTTACCAGGGGTATGGCTTTTGGCTGCATATTAAGGAGAGAACCTTATCCGTGTCTCTGG
    TGAAAAATAGCCTAGAAATCCAGAGGTGCTCTCAAGATTTGGAATCTGATAAAGAGACCTTCTTGATAGC
    TTTAGAGCAGCATAATCATAGTTTATCTTTGTTTGTCGATGGTACGACTTGGCTTATCCATATGAATTAT
    CTGCCAAGTCGTAGTGGGCGAGTCGCTATCATAGTTCGCGATATGGAAGATATCCTGGAAGATATAGGCA
    TTTTTGAAAGTAGTGGCTCTTTGAGGGTCAGTTGTCTTGCTGTTCCTGACGCTTTTCTTGCTGAGAAGTT
    ATATGATCGCGCTTTAGTGCTTTACCGAAGGATCGCAGAATCTTTCCCAGGACGTAAAGAAGGTTATGAA
    GCAAGGTTCAGAGCAGGAATTACAGTTTTAGAGAAGGCCTCTACAGATAATAATGAACAGGAATTTGCTC
    TAGCCATTGAAGAATTCTCAAAATTACATGACGGGGTTGCTGCTCCCTTAGAATACCTTGGTAAGGCTTT
    AGTATATCAGAGACTCCAAGAGTATAATGAAGAAATTAAGAGTTTGCTATTAGCATTGAAACGTTATTCG
    CAGCATCCTGAAATCTTTAGGCTTAAAGACCATGTGGTTTACCGACTCCATGAGAGCTTTTATAAACGGG
    ATCGCCTTGCTCTGGTGTTCATGATTTTAGTATTGGAAATAGCTCCCCAGGCAATCACTCCAGGGCAGGA
    AGAAAAAATCCTGGTTTGGTTAAAGGACAAATCTCGGGCTACCTTATTTTGCCTCCTGGATCCCACGGTC
    TTAGAGCTGCGCTCTTCTAAAATGGAATTATTTTTAAGTTATTGGTCTGGGTTTATTCCCCATCTCAATA
    GTCTATTTCATAGAGCTTGGGATCAAAGCGATGTGCGAGCTTTGATCGAGATTTTCTATGTTGCTTGTGA
    TCTTCATAAATGGCAGTTTCTCTCTTCTTGTATCGACATATTTAAAGAGTCTCTTGAGGATCAGAAAGCC
    ACAGAAGAGATTGTTGAGTTCTCTTTCGAGGATTTAGGGGCATTTCTTTTTGCTATTCAGAGCATCTTTA
    ACAAGGAAGATGCAGAGAAGATCTTTGTTTCTAATGATCAATTATCGCCAATCCTTCTTGTTTATATATT
    CGATCTTTTTGCAAATCGTGCTCTTCTGGAATCTCAAGGAGAGGCTATTTTTCAGGCTTTGGATCTCATC
    CGAAGTAAAGTTCCTGAAAATTTTTATCATGATTACTTGCGGAATCATGAAATCCGAGCGCATCTTTGGT
    GCCGCAATGAGAAGGCTCTAAGCACGATTTTTGAAAACTATACAGAGAAACAGCTAAAGGATGAGCAACA
    TGAACTGTTCGTTCTCTATGGATGTTACCTTGCTCTTATACAAGGTGCTGAGGCGGCAAAGCAGCATTTT
    GATGTATGTCGTGAAGATCGCATTTTCCCTGCTTCATTATTAGCTAGAAATTACAATCGTTTAGGTCTTC
    CCAAAGATGCTCTTAGCTATCAAGAGCGGCGTTTGTTATTGCGACAAAAGTTTCTCTATTTCCATTGTCT
    TGGTAACCACGACGAGCGTGACTTATGCCAGACTATGTATCACCTCTTAACCGAAGAATTTCAGCTTTA
    
    
  • Protein Sequence : Show Sequence
    >NP_224303.2 serine/threonine protein kinase [Chlamydia pneumoniae CWL029]
    MERYDIVRIIGKGGMGEVYLAYDPVCSRKVALKKIREDLAENPLLKRRFLREARIAADLIHPGVVPVYTI
    YSEKDPVYYTMPYIEGYTLKTLLKSVWQKESLSKELAEKTSVGAFLSIFHKICCTIEYVHSRGILHRDLK
    PDNILLGLFSEAVILDWGAAVACGEEEDLLDIDVSKEEVLSSRMTIPGRIVGTPDYMAPERLLGHPASKS
    TDIYALGVVLYQMLTLSFPYRRKKGKKIVLDGQRIPSPQEVAPYREIPPFLSAVVMRMLAVDPQERYSSV
    TELKEDIESHLKGSPKWTLTTALPPKKSSSWKLNEPILLSKYFPMLEVSPASWYSLAISNIESFSEMRLE
    YTLSKKGLNEGFGILLPTSENALGGDFYQGYGFWLHIKERTLSVSLVKNSLEIQRCSQDLESDKETFLIA
    LEQHNHSLSLFVDGTTWLIHMNYLPSRSGRVAIIVRDMEDILEDIGIFESSGSLRVSCLAVPDAFLAEKL
    YDRALVLYRRIAESFPGRKEGYEARFRAGITVLEKASTDNNEQEFALAIEEFSKLHDGVAAPLEYLGKAL
    VYQRLQEYNEEIKSLLLALKRYSQHPEIFRLKDHVVYRLHESFYKRDRLALVFMILVLEIAPQAITPGQE
    EKILVWLKDKSRATLFCLLDPTVLELRSSKMELFLSYWSGFIPHLNSLFHRAWDQSDVRALIEIFYVACD
    LHKWQFLSSCIDIFKESLEDQKATEEIVEFSFEDLGAFLFAIQSIFNKEDAEKIFVSNDQLSPILLVYIF
    DLFANRALLESQGEAIFQALDLIRSKVPENFYHDYLRNHEIRAHLWCRNEKALSTIFENYTEKQLKDEQH
    ELFVLYGCYLALIQGAEAAKQHFDVCREDRIFPASLLARNYNRLGLPKDALSYQERRLLLRQKFLYFHCL
    GNHDERDLCQTMYHLLTEEFQL
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : IM-ID vaccination with CPn0095 (pknD) generated a response that resulted in moderately, but significantly reduced total C. pneumoniae lung loads as compared to control mice vaccinated with a plasmid expressing a non-Chlamydia ORF (p ≤ 0.019). This resulted in the ability of CPN0095 to mediate a moderate, but statistically significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).
  • Related Vaccine(s): C. pneumoniae DNA vaccine encoding PknD
12. pmp10
  • Gene Name : pmp10
  • Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
  • NCBI Protein GI : CRI43239
  • Other Database IDs : CDD:284879
    CDD:214872
  • Taxonomy ID : 83558
  • Protein Name : Probable outer membrane protein pmp10
  • Protein pI : 6.3
  • Protein Weight : 80326.05
  • Protein Length : 858
  • Protein Note : Chlamydia polymorphic membrane protein middle domain; pfam07548
  • Protein Sequence : Show Sequence
    >CRI43239.1 Probable outer membrane protein pmp10 [Chlamydia pneumoniae]
    MLFSKNFSTDNGGAITAKTLSLTGTTMSALFSENTSSKKGGAIQTSDALTITGNQGEVSFSDNTSSDSGA
    AIFTEASVTISNNAKVSFIDNKVTGASSSTTGDMSGGAICAYKTSTDTKVTLTGNQMLLFSNNTSTTAGG
    AIYVKKLELASGGLTLFSRNSVNGGTAPKGGAIAIEDSGELSLSADSGDIVFLGNTVTSTTPGTNRSSID
    LGTSAKMTALRSAAGRAIYFYDPITTGSSTTVTDVLKVNETPADSALQYTGNIIFTGEKLSETEAADSKN
    LTSKLLQPVTLSGGTLSLKHGVTLQTQAFTQQADSRLEMDVGTTLEPADTSTINNLVINISSIDGAKKAK
    IETKATSKNLTLSGTITLLDPTGTFYENHSLRNPQSYDILELKASGTVTSTAVTPDPIMGEKFHYGYQGT
    WGPIVWGTGASTTATFNWTKTGYIPNPERIGSLVPNSLWNAFIDISSLHYLMETANEGLQGDRAFWCAGL
    SNFFHKDSTKTRRGFRHLSGGYVIGGNLHTCSDKILSAAFCQLFGRDRDYFVAKNQGTVYGGTLYYQHNE
    TYISLPCKLRPCSLSYVPTEIPVLFSGNLSYTHTDNDLKTKYTTYPTVKGSWGNDSFALEFGGRAPICLD
    ESALFEQYMPFMKLQFVYAHQEGFKEQGTEAREFGSSRLVNLALPIGIRFDKESDCQDATYNLTLGYTVD
    LVRSNPDCTTTLRISGDSWKTFGTNLARQALVLRAGNHFCFNSNFEAFSQFSFELRGSSRNYNVDLGAKY
    QF
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Finco et al., 2005)
13. pmp2
  • Gene Name : pmp2
  • Sequence Strain (Species/Organism) : Chlamydophila pneumoniae
  • NCBI Protein GI : CRI43236
  • Other Database IDs : CDD:273587
    CDD:284879
    CDD:214872
  • Taxonomy ID : 83558
  • Protein Name : Probable outer membrane protein pmp2
  • Protein pI : 5.53
  • Protein Weight : 68063.2
  • Protein Length : 763
  • Protein Note : Chlamydial polymorphic outer membrane protein repeat; TIGR01376
  • Protein Sequence : Show Sequence
    >CRI43236.1 Probable outer membrane protein pmp2, partial [Chlamydia pneumoniae]
    MKIPLRFLLISLVPTLSMSNLLGAATTEELSASNSFDGTTSTTSFSSKTSSATDGTNYVFKDSVVIENVP
    KTGETQSTSCFKNDAAAGDLNFLGGGFSFTFSNIDATTASGAAIGSEAANKTVTLSGFSALSFLKSPAST
    VTNGLGAINVKGNLSLLDNDKVLIQDNFSTGDGGAINCAGSLKIANNKSLSFIGNSSSTRGGAIHTKNLT
    LSSGGETLFQGNTAPTAAGKGGAIAIADSGTLSISGDSGDIIFEGNTIGATGTVSHSAIDLGTSAKITAL
    RAAQGHTIYFYDPITVTGSTSVADALNINSPDTGDNKEYTGTIVFSGEKLTEAEAKDEKNRTSKLLQNVA
    FKNGTVVLKGDVVLSANGFSQDANSKLIMDLGTSLVANTESIELTNLEINIDSLRNGKKIKLSAATAQKD
    IRIDRPVVLAISDESFYQNGFLNEDHSYDGILELDAGKDIVISADSRSIDAVQSPYGYQGKWTINWSTDD
    KKATVSWAKQSFNPTAEQEAPLVPNLLWGSFIDVRSFQNFIELGTEGAPYEKRFWVAGISNVLHRSGREN
    QRKFRHVSGGAVVGASTRMPGGDTLSLGFAQLFARDKDYFMNTNFAKTYAGSLRLQHDASLYSVVSILLG
    EGGLREILLPYVSKTLPCSFYGQLSYGHTDHRMKTESLPPP
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Finco et al., 2005)
14. Ssb
  • Gene Name : Ssb
  • Sequence Strain (Species/Organism) : Chlamydophila pneumoniae CWL029
  • VO ID : VO_0010896
  • NCBI Gene ID : 894901
  • NCBI Protein GI : 15618301
  • Locus Tag : CPn0386
  • Genbank Accession : AE001363
  • Protein Accession : NP_224586
  • Taxonomy ID : 115713
  • Gene Starting Position : 434042
  • Gene Ending Position : 434524
  • Gene Strand (Orientation) : -
  • Protein Name : single-stranded DNA-binding protein
  • Protein pI : 5.29
  • Protein Weight : 16434.22
  • Protein Length : 160
  • Protein Note : binds to single stranded DNA and may facilitate the binding and interaction of other proteins to DNA
  • DNA Sequence : Show Sequence
    >NC_000922.1:434042-434524 Chlamydophila pneumoniae CWL029 chromosome, complete genome
    ATTAAAAAGGAACATCTTCACAGACATACTGCTGTTCTTGACCATAACCAGCATACATATCTTTATCTTT
    AATAGCTTCTGCGTCCAGTGCTTCACCTTCAAACCCTACGGATACAGATTCATATCCCACTTGCTGATGA
    TTGTCTTCTAAAGATGGAGAACGGCTGCCTTCATTGCGACCGAAAGGACTGAATTTCAAAGAATCTACAC
    TAATCACTAAAGAAGATTGCGGTGAACCATCTTTGCTCATGTAACTCTCTACAGAGATATCGCCAGCAAC
    AATGACTCCTGAGCCTTTCTTCAAGTAAGGAAGCATCTTATCATAGCGATTGTGCCAAATATTGCATTTG
    CACCAAACAGTTTCATCTTTCATTCCAACTCGAGTCTTCACTCCCAGTCTCAGAGTGATCACACGTTTTC
    CTTTGGAAGTCATTCGCTCTTCAGGATCTGCTCCAAGGTAACCAGCAAAATGCCCAAACATCA
    
    
  • Protein Sequence : Show Sequence
    >NP_224586.1 single-stranded DNA-binding protein [Chlamydia pneumoniae CWL029]
    MMFGHFAGYLGADPEERMTSKGKRVITLRLGVKTRVGMKDETVWCKCNIWHNRYDKMLPYLKKGSGVIVA
    GDISVESYMSKDGSPQSSLVISVDSLKFSPFGRNEGSRSPSLEDNHQQVGYESVSVGFEGEALDAEAIKD
    KDMYAGYGQEQQYVCEDVPF
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice vaccinated with candidate gene ssb showed significant reduction of spleen chlamydial loads as compared to naïve, non-protected control mice (p ≤ 0.048). This resulted in the ability of ssb to mediate a modest, but significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).
  • Related Vaccine(s): C. pneumoniae DNA vaccine encoding Ssb
1. Ifng (Interferon gamma)
  • Gene Name : Ifng (Interferon gamma)
  • Sequence Strain (Species/Organism) : Mouse
  • NCBI Gene ID : 15978
  • NCBI Protein GI : 33468859
  • Genbank Accession : NM_008337
  • Protein Accession : NP_032363.1
  • Other Database IDs : MGI:107656; UniProt: P01580
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • DNA Sequence : Show Sequence
    >gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
    ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
    GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
    GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
    AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
    AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
    GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
    AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
    GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
    AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
    AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
    AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
    TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
    TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
    ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
    GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
    AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
    TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
    AGTATACCTTTGCAACC
  • Protein Sequence : Show Sequence
    >gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
    MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
    ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
    LLPESSLRKRKRSRC
  • Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
  • Additional Molecule Role : Vaximmutor
  • Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
  • Related Vaccine(s): C. pneumoniae CopN protein vaccine , C. pneumoniae LcrE protein vaccine
2. IgA
IV. Vaccine Information
1. C. pneumoniae CopN protein vaccine
a. Vaccine Ontology ID:
VO_0011432
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
C. pneumoniae copN
e. Gene Engineering of copN
  • Type: Recombinant protein preparation
  • Description: C. pneumoniae CopN (gene lcrE; position 0324 of C. pneumoniae CWL029), was produced in a Bacillus subtilis protein expression system as a soluble protein. Recombinant CopN protein was dissolved in PBS at a concentration of 1 mg/ml and heated to 100 °C for 10 min after which the visible precipitation of protein was discernible. C. pneumoniae preparation was boiled for 10 min in a water bath at a concentration of 2.5 × 107 IFU/ml in SPG. E. coli heat-labile toxin, LT (kindly provided by Prof. G. Dougan, Imperial Collage, London, UK) was added to heat-aggregated protein suspension to a final concentration of 12.5 μg/ml (Tammiruusu et al., 2007).
  • Detailed Gene Information: Click here.
f. Adjuvant: E. coli heat-labile toxin, LT
g. Immunization Route
Intranasal
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were immunized intranasally with 40 μg of heat-aggregated CopN/ 40 μl dose or 106 heat-treated C. pneumoniae inclusion forming unit (IFU) (approximately 1 μg of protein)/40 μl dose. Mice immunized intranasally with disrupted HL cells (Mock) or PBS were used as control. Fourteen days after the first immunization, the mice were boosted once with the same dose of antigen. All immunizations were performed under methoxyflurane anaesthesia (Metofane, Pitman-Moore, Mundelein, IL, USA) (Tammiruusu et al., 2007).
  • Challenge Protocol: At 14 days after the second immunization, the mice were challenged intranasally with 105 IFU of C. pneumoniae in 40 μl of SPG under Metofane anaesthesia. At certain time points after infection, three to six mice were sacrificed, lungs were mechanically homogenized in SPG and dilutions of lung supernatant were cultured on HL cell monolayers (Tammiruusu et al., 2007).
  • Efficacy: Intranasal immunization of BALB/c mice with heat-aggregated CopN protein and an Escherichia coli heat-labile toxin (LT) induced a strong immune response. The immunization induced statistically significant protection against intranasal C. pneumoniae challenge, the level of which correlated with the magnitude of CopN-specific lymphocyte proliferation (Tammiruusu et al., 2007).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: The results of this study showed that intranasal immunization of BALB/c mice with heat-aggregated CopN protein and an Escherichia coli heat-labile toxin (LT) induced a strong immune response, detected as IFN-gamma production. The response was significant as compared to PBS-vaccinated mice in the lungs, spleen, and mediastinal lymph nodes 14 days after challenge (Tammiruusu et al., 2007).
    • Detailed Gene Information: Click here.
2. C. pneumoniae DNA vaccine encoding FabD
a. Vaccine Ontology ID:
VO_0011434
b. Type:
DNA vaccine
c. Status:
Research
d. Antigen
C. pneumoniae fabD
e. Gene Engineering of FabD
  • Type: DNA vaccine construction
  • Description: The genome sequence of C. pneumoniae isolate CDC/CWL-029 (ATCC strain VR-1310) was extracted from Genbank (AE001363, 1,230,230 bp). The 1052 annotated genes of C. pneumoniae were imported into a gene-splitting and primer prediction program; primer pairs to amplify 1263 ORFs of 1.5 kb or less were exported. A 1.5 kb maximum ORF length was chosen to ensure sufficient PCR quality and yields, and this generated a few additional fragments (Li et al., 2006).
  • Detailed Gene Information: Click here.
f. Vector:
pCMVi-UB or linear expression elements (Li et al., 2006)
g. Immunization Route
Intramuscular injection (i.m.)
h. Mouse Response
  • Host Strain: A/J
  • Vaccination Protocol: For intranasal inoculation, mice received a light isoflurane inhalation anesthesia. Vaccine protection control mice were inoculated with a low dose of 5 × 106 C. pneumoniae elementary bodies in 30 μl SPG buffer (Li et al., 2006).
  • Challenge Protocol: High-dose challenge infection was performed 4 weeks after the last gene gun genetic vaccination or low dose inoculation of live C. pneumoniae, and 6 weeks after the last intramuscular-intradermal genetic vaccination, by intranasal inoculation of 1 × 108 C. pneumoniae elementary bodies in 30 μl SPG buffer. Mice were sacrificed by CO2 inhalation 2 h, 3 days, 10 days, or 15 days after inoculation, and lungs and spleen were weighed, snap frozen in liquid nitrogen, and stored at −80 °C until further processing (Li et al., 2006).
  • Efficacy: M-ID vaccination with fabD generated a response that resulted in moderately, but significantly reduced total C. pneumoniae lung loads as compared to control mice vaccinated with a plasmid expressing a non-Chlamydia ORF (p ≤ 0.019). This resulted in the ability of fabD to mediate a moderate, but statistically significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).
3. C. pneumoniae DNA vaccine encoding PknD
a. Vaccine Ontology ID:
VO_0011424
b. Type:
DNA vaccine
c. Status:
Research
d. Antigen
C. pneumoniae serine/threonine-protein kinase, PknD
e. Gene Engineering of PknD
  • Type: DNA vaccine construction
  • Description: The genome sequence of C. pneumoniae isolate CDC/CWL-029 (ATCC strain VR-1310) was extracted from Genbank (AE001363, 1,230,230 bp). The 1052 annotated genes of C. pneumoniae were imported into a gene-splitting and primer prediction program; primer pairs to amplify 1263 ORFs of 1.5 kb or less were exported. A 1.5 kb maximum ORF length was chosen to ensure sufficient PCR quality and yields, and this generated a few additional fragments (Li et al., 2006).
  • Detailed Gene Information: Click here.
f. Vector:
pCMVi-UB or linear expression elements (Li et al., 2006)
g. Immunization Route
Intramuscular injection (i.m.)
h. Mouse Response
  • Host Strain: A/J
  • Vaccination Protocol: For intranasal inoculation, mice received a light isoflurane inhalation anesthesia. Vaccine protection control mice were inoculated with a low dose of 5 × 106 C. pneumoniae elementary bodies in 30 μl SPG buffer (Li et al., 2006).
  • Challenge Protocol: High-dose challenge infection was performed 4 weeks after the last gene gun genetic vaccination or low dose inoculation of live C. pneumoniae, and 6 weeks after the last intramuscular-intradermal genetic vaccination, by intranasal inoculation of 1 × 108 C. pneumoniae elementary bodies in 30 μl SPG buffer. Mice were sacrificed by CO2 inhalation 2 h, 3 days, 10 days, or 15 days after inoculation, and lungs and spleen were weighed, snap frozen in liquid nitrogen, and stored at −80 °C until further processing (Li et al., 2006).
  • Efficacy: IM-ID vaccination with CPn0095 (pknD) generated a response that resulted in moderately, but significantly reduced total C. pneumoniae lung loads as compared to control mice vaccinated with a plasmid expressing a non-Chlamydia ORF (p ≤ 0.019). This resulted in the ability of CPN0095 to mediate a moderate, but statistically significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).
4. C. pneumoniae DNA vaccine encoding Ssb
a. Vaccine Ontology ID:
VO_0011425
b. Type:
DNA vaccine
c. Status:
Research
d. Antigen
C. pneumoniae single-stranded DNA-binding protein, ssb
e. Gene Engineering of Ssb
  • Type: DNA vaccine construction
  • Description: The genome sequence of C. pneumoniae isolate CDC/CWL-029 (ATCC strain VR-1310) was extracted from Genbank (AE001363, 1,230,230 bp). The 1052 annotated genes of C. pneumoniae were imported into a gene-splitting and primer prediction program; primer pairs to amplify 1263 ORFs of 1.5 kb or less were exported. A 1.5 kb maximum ORF length was chosen to ensure sufficient PCR quality and yields, and this generated a few additional fragments (Li et al., 2006).
  • Detailed Gene Information: Click here.
f. Vector:
pCMVi-UB or linear expression elements (Li et al., 2006)
g. Immunization Route
Intramuscular injection (i.m.)
h. Mouse Response
  • Host Strain: A/J
  • Vaccination Protocol: For intranasal inoculation, mice received a light isoflurane inhalation anesthesia. Vaccine protection control mice were inoculated with a low dose of 5 × 106 C. pneumoniae elementary bodies in 30 μl SPG buffer (Li et al., 2006).
  • Challenge Protocol: High-dose challenge infection was performed 4 weeks after the last gene gun genetic vaccination or low dose inoculation of live C. pneumoniae, and 6 weeks after the last intramuscular-intradermal genetic vaccination, by intranasal inoculation of 1 × 108 C. pneumoniae elementary bodies in 30 μl SPG buffer. Mice were sacrificed by CO2 inhalation 2 h, 3 days, 10 days, or 15 days after inoculation, and lungs and spleen were weighed, snap frozen in liquid nitrogen, and stored at −80 °C until further processing (Li et al., 2006).
  • Efficacy: Mice vaccinated with candidate gene ssb showed significant reduction of spleen chlamydial loads as compared to naïve, non-protected control mice (p ≤ 0.048). This resulted in the ability of ssb to mediate a modest, but significant level of protection in an inbred A/J mouse respiratory challenge model (Li et al., 2006).
5. C. pneumoniae DNA vaccine pHSP-60
a. Vaccine Ontology ID:
VO_0004555
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of groES
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
f. Vector:
pCI (Svanholm et al., 2000)
g. Immunization Route
intranasal immunization
h. Mouse Response
  • Vaccine Immune Response Type: VO_0003057
  • Efficacy: Immunization with this vaccine resulted in signi®cant protection, as measured by a lower bacterial load and a less severe pathological outcome after infection with C. pneumoniae (Svanholm et al., 2000).
6. C. pneumoniae LcrE protein vaccine
a. Vaccine Ontology ID:
VO_0011435
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
C. pneumoniae LcrE
e. Gene Engineering of LcrE
  • Type: Recombinant protein preparation
  • Description: A 1218-kb DNA fragment containing the lcrE gene (GenBank ID 15618244, Locus tag CPn0324) was amplified by PCR, using C. pneumoniae (CWL029 ATCC) DNA as template. The PCR was performed in a GeneAmp II (Applied Biosystems, Foster City, CA, USA) thermocycler with Advantage GC cDNA polymerase (Clontech, Mountain View, CA, USA), and the amplification conditions were set as recommended by the manufacturer. The amplicon was digested with NdeI and BamHI and inserted into p6HisF-11d (icl) pET vector by digesting it with the same enzymes and replacing the icl gene (Faludi et al., 2009).
  • Detailed Gene Information: Click here.
f. Adjuvant: aluminum vaccine adjuvant
  • VO ID: VO_0000884
  • Description: Either Freund's or Alum adjuvants
g. Adjuvant: Freunds emulsified oil adjuvant
h. Immunization Route
Subcutaneous injection
i. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: The mice in groups of 25 were immunized subcutaneously into the tail base with the purified LcrE protein diluted in phosphate buffered saline (PBS) at a dose of 20 μg mixed with 25 μl Alum (Aluminum hydroxide Gel, Sigma) or 75 μl Freund's adjuvants (Chemicon International, Temecula, CA, USA; 1st inoculation with complete and 2nd and 3rd inoculations with incomplete Freund's adjuvant) in 0.15-ml volume 3 times at 3-week intervals (Faludi et al., 2009).
  • Challenge Protocol: Two weeks after the last immunization, the immunized and non-immunized mice (absolute naive animals) were challenged with 4×105 inclusion forming unit (IFU) C. pneumoniae (CWL029, ATCC) in 25 μl PBS intranasally under pentobarbital sodium anesthesia (Faludi et al., 2009).
  • Efficacy: The immunogenicity and protective effect of recombinant LcrE protein combined either with Freund's or Alum adjuvant were investigated in mice. The immunization with both protocols resulted in a significant reduction of the number of viable C. pneumoniae in the lungs after challenge. Results confirm that LcrE induces protective immunity in mice (Faludi et al., 2009).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: The presence of LcrE-specific IFN-gamma-producing cells in LcrE+Alum-immunized mice indicates Th1 type response. IFN-gamma responses were measured in spleen cells collected 2 weeks after last immunization and were significantly higher than mock-immunized mice (Faludi et al., 2009).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgA
    • Gene Response: LcrE-specific IgA level was higher in both the sera and the lungs after using Freund's adjuvant than non-immunized mice at the time of the challenge (Faludi et al., 2009).
    • Detailed Gene Information: Click here.
V. References
1. Atanu et al., 2013: Atanu FO, Oviedo-Orta E, Watson KA. A novel transport mechanism for MOMP in Chlamydophila pneumoniae and its putative role in immune-therapy. PloS one. 2013; 8(4); e61139. [PubMed: 23637791].
2. Faludi and Szabó, 2011: Faludi I, Szabó ÁM. Vaccination with DNA vector expressing chlamydial low calcium response protein E (LcrE) against Chlamydophila pneumoniae infection. Acta microbiologica et immunologica Hungarica. 2011; 58(2); 123-134. [PubMed: 21715282].
3. Faludi et al., 2009: Faludi I, Burian K, Csanadi A, Miczak A, Lu X, Kakkar VV, Gonczol E, Endresz V. Adjuvant modulation of the immune response of mice against the LcrE protein of Chlamydophila pneumoniae. International journal of medical microbiology : IJMM. 2009; 299(7); 520-528. [PubMed: 19451031].
4. Finco et al., 2005: Finco O, Bonci A, Agnusdei M, Scarselli M, Petracca R, Norais N, Ferrari G, Garaguso I, Donati M, Sambri V, Cevenini R, Ratti G, Grandi G. Identification of new potential vaccine candidates against Chlamydia pneumoniae by multiple screenings. Vaccine. 2005; 23(9); 1178-1188. [PubMed: 15629361].
5. Li et al., 2006: Li D, Borovkov A, Vaglenov A, Wang C, Kim T, Gao D, Sykes KF, Kaltenboeck B. Mouse model of respiratory Chlamydia pneumoniae infection for a genomic screen of subunit vaccine candidates. Vaccine. 2006; 24(15); 2917-2927. [PubMed: 16434129].
6. Lu et al., 2012: Lu X, Xia M, Endresz V, Faludi I, Szabo A, Gonczol E, Mundkur L, Chen D, Kakkar V. Impact of multiple antigenic epitopes from ApoB100, hHSP60 and Chlamydophila pneumoniae on atherosclerotic lesion development in Apob(tm2Sgy)Ldlr(tm1Her)J mice. Atherosclerosis. 2012; 225(1); 56-68. [PubMed: 22959702].
7. PenttilÀ et al., 2000: PenttilÀ T, Vuola JM, Puurula V, Anttila M, Sarvas M, Rautonen N, MÀkelÀ PH, Puolakkainen M. Immunity to Chlamydia pneumoniae induced by vaccination with DNA vectors expressing a cytoplasmic protein (Hsp60) or outer membrane proteins (MOMP and Omp2). Vaccine. 2000; 19(9-10); 1256-1265. [PubMed: 11137265].
8. Svanholm et al., 2000: Svanholm C, Bandholtz L, Castaños-Velez E, Wigzell H, Rottenberg ME. Protective DNA immunization against Chlamydia pneumoniae. Scandinavian journal of immunology. 2000; 51(4); 345-353. [PubMed: 10736106].
9. Tammiruusu et al., 2007: Tammiruusu A, PenttilÀ T, Lahesmaa R, Sarvas M, Puolakkainen M, Vuola JM. Intranasal administration of chlamydial outer protein N (CopN) induces protection against pulmonary Chlamydia pneumoniae infection in a mouse model. Vaccine. 2007; 25(2); 283-290. [PubMed: 16949182].
10. Tammiruusu et al., 2007: Tammiruusu A, Penttilä T, Lahesmaa R, Sarvas M, Puolakkainen M, Vuola JM. Intranasal administration of chlamydial outer protein N (CopN) induces protection against pulmonary Chlamydia pneumoniae infection in a mouse model. Vaccine. 2007; 25(2); 283-290. [PubMed: 16949182].
11. Wiki: Chlamydophila pneumoniae: Chlamydophila pneumoniae [http://en.wikipedia.org/wiki/Chlamydophila_pneumoniae]