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Chlamydia muridarum

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Introduction
  2. Vaccine Related Pathogen Genes
    1. MOMP (Protective antigen)
    2. PmpE/F-2 (Protective antigen)
    3. PmpG-1 (Protective antigen)
    4. RplF (Protective antigen)
    5. TC0439 adherence factor (Protective antigen)
    6. TC0512 outer membrane protein, putative (Protective antigen)
    7. TC0757 hypothetical protein (Protective antigen)
    8. TC0767 hypothetical protein (Protective antigen)
    9. TC0768 hypothetical protein (Protective antigen)
  3. Vaccine Information
    1. C. muridarum DNA vaccine encoding TC0439
    2. C. muridarum DNA vaccine encoding TC0512
    3. C. muridarum DNA vaccine encoding TC0757
    4. C. muridarum DNA vaccine encoding TC0767/768
    5. C. muridarum MOMP protein vaccine
    6. C. muridarum PmpE/F-2 protein vaccine
    7. C. muridarum PmpG-1 protein vaccine
    8. C. muridarum RplF protein vaccine
  4. References
I. General Information
1. NCBI Taxonomy ID:
83560
2. Introduction
Chlamydia muridarum is an intracellular bacterial species that at one time belonged to Chlamydia trachomatis. However, C. trachomatis naturally only infects humans and C. muridarum naturally infects only members of the family Muridae (includes both mice and hamsters, Alderton, 1996). Two strains of Chlamydia muridarum, MoPn (originally named Nigg) and SFPD, have been isolated from mice and hamsters. Glycogen production by both strains has been demonstrated, although it is difficult to detect glycogen in MoPn (Moulder et al., 1984). Chlamydia muridarum MoPn binds mAbs recognizing Chlamydia trachomatis MOMP vs4 core epitope (T)LNPT(IA). DNA sequence analysis indicates that these mAbs should recognize SFPD and that Chlamydia trachomatis B-serogroup mAbs specific for the vs4 epitope IAGAG should recognize SFPD (Wiki: Chlamydia muridarum).
1. MOMP
  • Gene Name : MOMP
  • Sequence Strain (Speices/Organism) : Chlamydia muridarum
  • VO ID : VO_0010890
  • NCBI Protein GI : 1518659
  • Other Database IDs : CDD:144782
  • Taxonomy ID : 83560
  • Gene Strand (Orientation) : ?
  • Protein Name : major outer membrane protein
  • Protein pI : 4.69
  • Protein Weight : 39600.904
  • Protein Length : 387
  • Protein Note : mouse pneumonitis; submitted as Chlamydia trachomatis
  • Protein Sequence : Show Sequence 
    >gi|1518659|gb|AAB07068.1| major outer membrane protein [Chlamydia muridarum]
MKKLLKSVLAFAVLGSASSLHALPVGNPAEPSLMIDGILWEGFGGDPCDPCTTWCDAISLRLGYYGDFVF
DRVLKTDVNKQFEMGAAPTGDADLTTAPTPASRENPAYGKHMQDAEMFTNAAYMALNIWDRFDVFCTLGA
TSGYLKGNSAAFNLVGLFGRDETAVAADDIPNVSLSQAVVELYTDTAFAWSVGARAALWECGCATLGASF
QYAQSKPKVEELNVLCNAAEFTINKPKGYVGQEFPLNIKAGTVSATDTKDASIDYHEWQASLALSYRLNM
FTPYIGVKWSRASFDADTIRIAQPKLETSILKMTTWNPTISGSGIDVDTKITDTLQIVSLQLNKMKSRKS
CGLAIGTTIVDADKYAVTVETRLIDERAAHVNAQFRF
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Female BALB/c mice were immunized with chlamydial major outer membrane protein (MOMP) mixed with cholera toxin and CpG oligodeoxynucleotide adjuvants by either the IN or TCI routes. Both intranasal and transcutaneous immunization protected mice against respiratory challenge with Chlamydia (Skelding et al., 2006).
  • Related Vaccine(s): C. muridarum MOMP protein vaccine
2. PmpE/F-2
  • Gene Name : PmpE/F-2
  • Sequence Strain (Speices/Organism) : Chlamydia muridarum Nigg
  • VO ID : VO_0010893
  • NCBI Gene ID : 1246432
  • NCBI Protein GI : 15834882
  • Locus Tag : TC0262
  • Genbank Accession : NZ_ACOV01000003
  • Protein Accession : NP_296641
  • Taxonomy ID : 243161
  • Gene Starting Position : 308276
  • Gene Ending Position : 311353
  • Gene Strand (Orientation) : -
  • Protein Name : polymorphic membrane protein E/F family protein
  • Protein pI : 8.26
  • Protein Weight : 107782.38
  • Protein Length : 1025
  • Protein Note : similar to GP:4376753; identified by sequence similarity; putative
  • DNA Sequence : Show Sequence 
    >gi|29337300:308276-311353 Chlamydia muridarum Nigg, complete genome
GTTAAAAGACCAGAGCCCCTCCTGCATTCATGTAGTGTGAGACTGTAGAAGTAGCCACCTGCGCTTGATA
ATTAGCGAATAGTTTAAGATAAGAGAATTTTAGAGAAGATGATCCTCTCCCATAAAAGGAATGCTTAGCT
AAAGGAGTGTTTGTTGTTTCCCAAGAACCATTGTTTTTGATTAAAACGGTATTAAGAATGGGGCGCTTCC
AGTAAAGAGTAGGCTGGTATGCTACCTCCATTTCGCAGGATACAGTTGGCCATTTATTCGAAGAATAAAC
ACCTTTAATTCCTATTGGAGAAACAACAGCTGTATGTGGTTGTTTTAACGAAAATAATCTAGCCAGGTCT
CCCTGCTCCTGGATAGATGCAGGCTCTGTTCGGGAGATGAGAGCTTGAATGAATGGTGTCAACATAATCG
ATTGGAAAGGCATACTATCCCGCAGTTCGCAACGTAGAGAGCCCCCCAGAGTCGTAGAGTGAAAGTTCCC
TTTAGAAAACTTTTTCGTTCCATAGAAGTTATATGTTTTATGAGTACCATAGTTATAAGCAGCTGCTCCA
ATAAGAGCGATACGATCAAACATAGGTTGTTCAAAACACAGAGCGGAGAGATAATAACGAGAAGAAATTT
TATTATTGGTACGTTTCTCTTTCATTGTGTCGGAGGATTGGGAAAAGGAAAATAAAAACTTATGTCCGTG
TGCTCCTGATGCTTGTGAAGATATAGCATATCCTTTCGATGCAGAAGAATATCCGTGATAATCTAAGCGT
TTCTGCTGTTTGGTGAATAGCGTAGCTGCAGATCCTTGTAAAGCGAACCAAGAGTCATCTGGTAGTAATG
AACGTAAGTACAGGGTTGTATTTCTTCCAGAAGACACTAAATTATTCGCAATAAGATCCCCTCTACGAGC
AGGATCTGCTATATATCCGATTGGGGTCCAGCTCACTACTAAGGAGTGTCTAGTGACTGTTACGTCCGTT
GTAGCAAGAGGAGTTGTTCCTCCTGTAGAAGCTCTAATGGAAGGAATCTTAGCTGTAGTTGTACTGTTGG
AATCAAAAGTTTCGCTAGTGTTTTTTTGTTCTTGAATAGAATTGTTGGCTGTTTGAGTCTCTGTACTATT
GGTAAGAGAGACCGTACGGGTGGTTGTCATCCAATTAGATGTCCAGAGTCCTTGGTATCCGTAATGTGGT
AAAGAATATAGCGAGGTATTGAGAGTGTTTTTCCAATCATTGCTTAACTGCGCATCATTAGTCGTTTCGA
TATGTAAAATGGGTTGATTTGCTTTTCCTCGGGATAGGTCGCAGCTGTCATAAACTGATTCGTAATTTTC
ATCTACTAACGAGACAAGACCGGAGAAAAAGATCTTTTGAGTTGCCACGTTCTCTGTGCTTACTGGAGCT
TGGTTAGAGTTTGTATTCTGTGTCATATCTTGGGGATTAACACGAATACGTGGAGCTGTAGTACCTTGGT
CATTGAGAAGACCTGGCAAAATGATTCCAAGATGCGTAATAGTGAGAGAAGCGTCTTTCCCTACAGTAAG
AATAGAATCTTGTCCTAAAGCAAGAAGGCTAGTGCTTTCTTGAGTAAGAGGGCCATTGAAAAATTCAAAC
TCTGCGCCATTTTCTATGGACATAGTCCCAAACTTCAAGGTAGTGGGGGAGTCTTTAATTTGGTTATGAG
TTTTACTGGCAATCATGTGAGCTTGTAGATCACTGGATATGTTTTTGTATGAAAACACAACAGCGCCAGA
GAACTCATCCGAAGGATTGATGGTAACGGTGTGCGGGCTATTTTCAGGGGGTGTATTAGCTTTTTTTAAG
CCTCCCCATTGAAAAAGATCATAAAAATAGATGCGAGTATCTTTCTTAGCACCAAGAGATAGGGTGATAG
GGTTATTGTTGGTTGTTCCAGGAATATAAAAAAGTTTTCGCAAACCTGCTGACATTCCGGTAGTTGAAAG
CTCTGTATTATTGTTGAAGATAATATCCCCAGAGCCAGTATGAATATAGTTCGCAGCTGGGCTTGCAAAT
AGGTGAAAAGCTCCTCCATACAGAGCCGAATTATTAAGAAAATAGATAGGACCATTTTCTTGAAAATTAA
GGTTGTTGCAGGAAATAGCTCCTGCTATGTATGAGGCATTATTATTCTCAAAGTAGATAGGGCCTTTATT
ATTTAGAAGACCAAACTCTCCTCCTGCGCTTATAACTGCTCCATTTCCTTGAGCACTAGTGTTATGCCAT
CCTAAAGCGCTATTATTTATAAAAATAAGCTTATCCTTATTATCTCTAAAAATACACTGTCCCCCGGTGC
TGATAGCTCCTCCGTATCCTCCATAGTTGGAATTGAAGACTACCGGACCAGTATTGTGTGAGATTTCGCA
GGCACCTTGGCAGCCAACAGCTCCTCCTCGAGCAAGAATTGTAGCAAATGTGTTGGGTATGTGATCAGTA
GTATGGTTATTTTCAAAAGCAACTATTCCGGACGTATTTTTGATAACTAGGTTTTGGATTGTGGATACGG
CTCCTCCCATATCCCCAGAAGTGTTATTGATAAAATTTAAGCTATGATTATTAATTAGCTCGATGCTTGA
TCCGTATAAAGCCCCTCCGCGATTCCTTTCGGTTCTTAATGCTACTCCTGTAAATTGAGTTCCAATAGTT
GTATTATTATAGAACAATATGTTTTGGTTGTTTTTAAAGCTAATAAGGCCTGTAGCATTTATAGCTCCCC
CTCCTCCCTGATGGGTGCAATAATTGTTACAAAAAAACAAGTCTGCATTGTCAATGGCTGTGAAAGAAGT
CATAGACTCAACAAAAGCTCCACTAGAGTTTGTAGTGCTTAAACCTCTCGAAAACCATTGATCCTGCGTA
CAACTTGAAATAGTAAAGTTCCCTCTATAATTAGAAACATTTGCGTAAGTGATGTATTGAGCAGCAATGG
AGCTAGGCGGAGTAACTATGAATTCAATCTCTCTACCACCAAAAGTCAAGTTCGGAAGTTTGAGTGTATC
GGTTTCACTGGCCGAAATACAAGATAAAGGAATGAAAACAAGTGAAAGAGGGAGAATTCTGCGAGTCA
  • Protein Sequence : Show Sequence 
    >gi|15834882|ref|NP_296641.1| polymorphic membrane protein E/F family protein [Chlamydia muridarum Nigg]
MTRRILPLSLVFIPLSCISASETDTLKLPNLTFGGREIEFIVTPPSSIAAQYITYANVSNYRGNFTISSC
TQDQWFSRGLSTTNSSGAFVESMTSFTAIDNADLFFCNNYCTHQGGGGAINATGLISFKNNQNILFYNNT
TIGTQFTGVALRTERNRGGALYGSSIELINNHSLNFINNTSGDMGGAVSTIQNLVIKNTSGIVAFENNHT
TDHIPNTFATILARGGAVGCQGACEISHNTGPVVFNSNYGGYGGAISTGGQCIFRDNKDKLIFINNSALG
WHNTSAQGNGAVISAGGEFGLLNNKGPIYFENNNASYIAGAISCNNLNFQENGPIYFLNNSALYGGAFHL
FASPAANYIHTGSGDIIFNNNTELSTTGMSAGLRKLFYIPGTTNNNPITLSLGAKKDTRIYFYDLFQWGG
LKKANTPPENSPHTVTINPSDEFSGAVVFSYKNISSDLQAHMIASKTHNQIKDSPTTLKFGTMSIENGAE
FEFFNGPLTQESTSLLALGQDSILTVGKDASLTITHLGIILPGLLNDQGTTAPRIRVNPQDMTQNTNSNQ
APVSTENVATQKIFFSGLVSLVDENYESVYDSCDLSRGKANQPILHIETTNDAQLSNDWKNTLNTSLYSL
PHYGYQGLWTSNWMTTTRTVSLTNSTETQTANNSIQEQKNTSETFDSNSTTTAKIPSIRASTGGTTPLAT
TDVTVTRHSLVVSWTPIGYIADPARRGDLIANNLVSSGRNTTLYLRSLLPDDSWFALQGSAATLFTKQQK
RLDYHGYSSASKGYAISSQASGAHGHKFLFSFSQSSDTMKEKRTNNKISSRYYLSALCFEQPMFDRIALI
GAAAYNYGTHKTYNFYGTKKFSKGNFHSTTLGGSLRCELRDSMPFQSIMLTPFIQALISRTEPASIQEQG
DLARLFSLKQPHTAVVSPIGIKGVYSSNKWPTVSCEMEVAYQPTLYWKRPILNTVLIKNNGSWETTNTPL
AKHSFYGRGSSSLKFSYLKLFANYQAQVATSTVSHYMNAGGALVF
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. In addition, pmpE/F-2 was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009).
  • Related Vaccine(s): C. muridarum PmpE/F-2 protein vaccine
3. PmpG-1
  • Gene Name : PmpG-1
  • Sequence Strain (Speices/Organism) : Chlamydia muridarum Nigg
  • VO ID : VO_0010891
  • NCBI Gene ID : 1246433
  • NCBI Protein GI : 15834883
  • Locus Tag : TC0263
  • Genbank Accession : AE002160
  • Protein Accession : NP_296642
  • Taxonomy ID : 243161
  • Gene Starting Position : 311525
  • Gene Ending Position : 314488
  • Gene Strand (Orientation) : +
  • Protein Name : polymorphic membrane protein G family protein
  • Protein pI : 5.8
  • Protein Weight : 98416.45
  • Protein Length : 987
  • Protein Note : similar to GP:4376736; identified by sequence similarity; putative
  • DNA Sequence : Show Sequence 
    >gi|29337300:311525-314488 Chlamydia muridarum Nigg, complete genome
TGTGATGCAAACGCCTTTTCATAAGTTCTTTCTTCTAGCAATGCTATCTTACTCTTTATTGCAAGGAGGG
CATGCGGCAGATATTTCCATGCCTCCGGGAATTTATGATGGGACAACATTGACGGCGCCATTTCCCTACA
CTGTGATCGGAGATCCCAGAGGGACAAAGGTTACTTCATCGGGATCGCTAGAGTTGAAAAACCTGGACAA
TTCCATTGCGACTTTACCTCTAAGTTGTTTTGGTAATTTGTTGGGGAATTTCACTATTGCAGGAAGAGGG
CATTCGTTAGTATTTGAGAATATACGAACATCTACAAATGGGGCGGCATTGAGTAATCATGCTCCTTCTG
GACTGTTTGTAATTGAAGCTTTTGATGAACTCTCTCTTTTGAATTGTAATTCATTGGTATCTGTAGTTCC
TCAAACAGGGGGTACGACTACTTCTGTTCCTTCTAATGGGACGATCTATTCTAGAACAGATCTTGTTCTA
AGAGATATCAAGAAGGTTTCTTTCTATAGTAACTTAGTTTCTGGAGATGGGGGAGCTATAGATGCACAAA
GTTTAATGGTTAACGGAATTGAAAAACTTTGTACCTTCCAAGAAAATGTAGCGCAGTCCGATGGGGGAGC
GTGTCAGGTAACAAAGACCTTCTCTGCTGTGGGCAATAAGGTTCCTTTGTCTTTTTTAGGCAATGTTGCT
GGTAATAAGGGGGGAGGAGTTGCTGCTGTCAAAGATGGTCAGGGGGCAGGAGGGGCGACTGATCTATCGG
TTAATTTTGCCAATAATACTGCTGTAGAATTTGAGGGAAATAGTGCTCGAATAGGTGGAGGGATCTACTC
GGACGGAAATATTTCCTTTTTAGGGAATGCAAAGACAGTTTTCCTAAGTAACGTAGCTTCGCCTATTTAT
GTTGACCCTGCTGCTGCAGGAGGACAGCCCCCTGCAGATAAAGATAACTATGGAGATGGAGGAGCCATCT
TCTGCAAAAATGATACTAACATAGGTGAAGTCTCTTTCAAAGACGAGGGTGTTGTTTTCTTTAGTAAAAA
TATTGCCGCAGGAAAGGGGGGCGCTATTTATGCTAAGAAACTGACAATTTCTGACTGTGGTCCGGTCCAG
TTTCTTGGTAATGTCGCGAATGACGGGGGCGCTATTTATCTAGTAGATCAGGGGGAACTTAGTCTATCTG
CTGATCGCGGAGATATTATTTTTGATGGAAATTTAAAGAGAATGGCTACGCAAGGCGCTGCCACCGTCCA
TGATGTAATGGTTGCATCGAATGCTATCTCTATGGCTACAGGGGGGCAAATCACAACATTAAGGGCTAAG
GAAGGTCGCCGAATTCTTTTTAATGACCCTATTGAAATGGCGAATGGACAACCTGTAATACAAACTCTTA
CAGTAAACGAGGGCGAAGGATATACGGGGGACATTGTTTTTGCTAAAGGTGATAATGTTTTGTACTCAAG
TATTGAGCTGAGTCAGGGAAGAATTATTCTCCGAGAGCAAACAAAATTATTGGTTAACTCCCTGACTCAG
ACTGGAGGGAGTGTACATATGGAAGGGGGGAGTACACTAGACTTTGCAGTAACAACGCCACCAGCTGCTA
ATTCGATGGCTCTTACTAATGTACACTTCTCCTTAGCTTCTTTACTAAAAAATAATGGGGTTACAAATCC
TCCAACGAATCCTCCAGTACAGGTTTCTAGTCCAGCTGTAATTGGTAATACAGCTGCTGGTACTGTTACG
ATTTCTGGTCCGATCTTTTTTGAAGATTTAGATGAAACTGCTTACGATAATAATCAGTGGTTAGGTGCGG
ATCAAACTATTGATGTGCTGCAGTTGCATTTAGGAGCGAATCCTCCGGCTAACGCTCCAACTGATTTGAC
TTTAGGGAACGAAAGTTCTAAATATGGGTATCAAGGAAGTTGGACACTTCAATGGGAACCAGATCCTGCG
AATCCTCCACAGAACAATAGCTACATGTTGAAGGCAAGCTGGACTAAAACAGGTTATAATCCTGGTCCGG
AGCGCGTAGCTTCTCTGGTCTCTAATAGTCTTTGGGGATCCATTTTAGATGTGCGTTCCGCGCATTCTGC
GATTCAAGCAAGTATAGATGGACGAGCTTATTGTCGGGGTATTTGGATTTCTGGGATTTCGAACTTTTTC
TATCATGATCAGGATGCTTTAGGACAGGGGTATCGTCATATTAGTGGGGGATATTCGATAGGAGCAAACT
CTTATTTCGGGTCTTCTATGTTTGGACTTGCTTTTACTGAAACTTTTGGTAGGTCCAAAGATTATGTGGT
CTGTCGATCTAACGATCACACTTGTGTAGGCTCTGTTTACTTATCCACTAGACAAGCGTTATGCGGATCC
TGTTTATTTGGAGATGCTTTTGTTCGGGCGAGTTACGGATTTGGAAATCAGCATATGAAGACCTCTTATA
CATTTGCTGAAGAGAGTAATGTGCGTTGGGATAATAACTGTGTAGTGGGAGAAGTTGGAGCTGGGCTCCC
TATCATGCTCGCTGCATCTAAGCTTTATCTAAATGAGTTGCGTCCCTTCGTGCAAGCAGAGTTTGCTTAT
GCAGAGCATGAATCTTTTACAGAGAGAGGGGATCAGGCTAGGGAGTTTAAGAGTGGGCATCTTATGAATC
TATCTATTCCAGTTGGGGTGAAGTTTGATCGATGCTCTAGTAAACATCCTAACAAGTATAGTTTTATGGG
AGCTTATATCTGTGATGCTTACCGGTCCATTTCTGGAACGGAGACAACACTCCTGTCTCATAAAGAGACT
TGGACAACAGATGCTTTCCATTTAGCAAGGCATGGAGTTATGGTCAGAGGATCTATGTATGCTTCTTTAA
CAGGTAATATAGAAGTCTATGGCCATGGAAAATATGAATACAGGGATGCCTCTCGAGGGTATGGTTTAAG
TATTGGAAGTAAAATCCGATTCTA
  • Protein Sequence : Show Sequence 
    >gi|15834883|ref|NP_296642.1| polymorphic membrane protein G family protein [Chlamydia muridarum Nigg]
MMQTPFHKFFLLAMLSYSLLQGGHAADISMPPGIYDGTTLTAPFPYTVIGDPRGTKVTSSGSLELKNLDN
SIATLPLSCFGNLLGNFTIAGRGHSLVFENIRTSTNGAALSNHAPSGLFVIEAFDELSLLNCNSLVSVVP
QTGGTTTSVPSNGTIYSRTDLVLRDIKKVSFYSNLVSGDGGAIDAQSLMVNGIEKLCTFQENVAQSDGGA
CQVTKTFSAVGNKVPLSFLGNVAGNKGGGVAAVKDGQGAGGATDLSVNFANNTAVEFEGNSARIGGGIYS
DGNISFLGNAKTVFLSNVASPIYVDPAAAGGQPPADKDNYGDGGAIFCKNDTNIGEVSFKDEGVVFFSKN
IAAGKGGAIYAKKLTISDCGPVQFLGNVANDGGAIYLVDQGELSLSADRGDIIFDGNLKRMATQGAATVH
DVMVASNAISMATGGQITTLRAKEGRRILFNDPIEMANGQPVIQTLTVNEGEGYTGDIVFAKGDNVLYSS
IELSQGRIILREQTKLLVNSLTQTGGSVHMEGGSTLDFAVTTPPAANSMALTNVHFSLASLLKNNGVTNP
PTNPPVQVSSPAVIGNTAAGTVTISGPIFFEDLDETAYDNNQWLGADQTIDVLQLHLGANPPANAPTDLT
LGNESSKYGYQGSWTLQWEPDPANPPQNNSYMLKASWTKTGYNPGPERVASLVSNSLWGSILDVRSAHSA
IQASIDGRAYCRGIWISGISNFFYHDQDALGQGYRHISGGYSIGANSYFGSSMFGLAFTETFGRSKDYVV
CRSNDHTCVGSVYLSTRQALCGSCLFGDAFVRASYGFGNQHMKTSYTFAEESNVRWDNNCVVGEVGAGLP
IMLAASKLYLNELRPFVQAEFAYAEHESFTERGDQAREFKSGHLMNLSIPVGVKFDRCSSKHPNKYSFMG
AYICDAYRSISGTETTLLSHKETWTTDAFHLARHGVMVRGSMYASLTGNIEVYGHGKYEYRDASRGYGLS
IGSKIRF
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. Overall, vaccination with DCs transfected with PmpG-1(25-500) exhibited the greatest degree of protective immunity among the four Chlamydia Ags tested (Yu et al., 2009).
  • Related Vaccine(s): C. muridarum PmpG-1 protein vaccine
4. RplF
  • Gene Name : RplF
  • Sequence Strain (Speices/Organism) : Chlamydia muridarum Nigg
  • VO ID : VO_0010892
  • NCBI Gene ID : 1246168
  • NCBI Protein GI : 15835415
  • Locus Tag : TC0801
  • Genbank Accession : AE002160
  • Protein Accession : NP_297174
  • Taxonomy ID : 243161
  • Gene Starting Position : 941594
  • Gene Ending Position : 942145
  • Gene Strand (Orientation) : -
  • Protein Name : 50S ribosomal protein L6
  • Protein pI : 10.64
  • Protein Weight : 18060.75
  • Protein Length : 183
  • Protein Note : ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance
  • DNA Sequence : Show Sequence 
    >gi|29337300:941594-942145 Chlamydia muridarum Nigg, complete genome
TCTATTTTTTACCAGTTTTCGCTGCTTTTCCAGCCTTACGACGTACATATTCGTTTTCGTAACGAATTCC
TTTACCTTTGTATGGCTCTGGAGGGCGTTTTGCACGAACACAAGCCGCAAATTCTCCTACCAACTGCTTG
TTGATACCTTTGATGGAGATCAATGTGTTCTTCTCAACAGAGACTTCCAATCCTGTAGGAATAGGCATTT
TTGTTGGATGGGAAACCCCTATCGATAAATCTAAGAAGGACCCCTGTACCGAAGCTCTGAATCCAACTCC
GATCATTTCTAAACGTTTTTCAAATCCAAGGTGGACACCTTTGACCATATTTGCTATCAAAGCCCAATAA
AGCCCTTGCATACGACCAGGTCTGTCTATAATGTGAGCTGCGGGAGAAACAAACACCTCATTACCTTTAA
CGGCAATCTCAACTTCTTTAGCCAACACCTGTGTCAAAGACCCTTTAGGACCTTTTACTGAGATTTCATC
ATTTTGAATAGAGACCTCTACGCCTTGAGGAAGTACAATAGGGTCTCGAGCTTTACGAGACA
  • Protein Sequence : Show Sequence 
    >gi|15835415|ref|NP_297174.1| 50S ribosomal protein L6 [Chlamydia muridarum Nigg]
MSRKARDPIVLPQGVEVSIQNDEISVKGPKGSLTQVLAKEVEIAVKGNEVFVSPAAHIIDRPGRMQGLYW
ALIANMVKGVHLGFEKRLEMIGVGFRASVQGSFLDLSIGVSHPTKMPIPTGLEVSVEKNTLISIKGINKQ
LVGEFAACVRAKRPPEPYKGKGIRYENEYVRRKAGKAAKTGKK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. In addition, RplF was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009).
  • Related Vaccine(s): C. muridarum RplF protein vaccine
5. TC0439 adherence factor
  • Gene Name : TC0439 adherence factor
  • Sequence Strain (Speices/Organism) : Chlamydia muridarum Nigg
  • VO ID : VO_0010887
  • NCBI Gene ID : 1245792
  • NCBI Protein GI : 15835057
  • Locus Tag : TC0439
  • Genbank Accession : AE002160
  • Protein Accession : NP_296816
  • Taxonomy ID : 243161
  • Gene Starting Position : 526698
  • Gene Ending Position : 536375
  • Gene Strand (Orientation) : +
  • Protein Name : adherence factor
  • Protein pI : 7.3
  • Protein Weight : 344966.97
  • Protein Length : 3225
  • Protein Note : similar to GP:6013469; identified by sequence similarity; putative
  • DNA Sequence : Show Sequence 
    >gi|29337300:526698-536375 Chlamydia muridarum Nigg, complete genome
CTTGGAACAAGCATTGTGTACTATGACTCTTTCGAATACAGCCTCTTCCACAGTTTCACCTAAGTCTACG
CAACTTAACCATACTTTATCCAATCAAACTCACCCTGTTGCTCAAAGCAATAGGAATATAGATTTCCAAA
TCTATGATGAACAGATTCATAATAATCATTCTACAGAAGATGTAGTTGCTATAGGACGTCGTATTCAGCA
AGAATATGCGAATCTTACCACATCAAAACAGGTTAATTTCTCGGCATCTCCATCTAACCATACTGGAAAC
TGGAAGATTTCTTTGCTTTATAATTTATCGATGCTGGTTGCAAATCTATTCCCTACAACTATTCAGCCTG
TGCAACCTCAAAAAGTCATCTTTAAGAATCAAGACTCTTCTAAGGAAACCCAACTGACCAAATCATCCAA
AGTAGTTTCTTCTCGCTCTTCTTTAAAAGAGATTTTTTCGAAGAAATCTCCTCCTTTGAGAAAGTCTTTT
TCTTCCTCTAAAATTTCTAGAGCAATCCCTAGAAGAAAAAAACGATCAAACGATGATCCAGTTCAAGCCA
CAAATACTTATGATTTGACCGCTGAAAATATTCTTGAAAAACTCTCTTTAACACAAGAACAACAAATTAA
GCACGATAATCTGATTAGCAATCTCAAAGAAGCAATTAATCGATATAGTGATCTTAATCGGAAAAATTCC
CGGAAAGGACAATCCCTTCTTGTTAGGCAAGCCAAAATTCTAGATGAAATTCTTTCACAGACTAAATCTA
CAGAGGAACGAGCTTCCAATTCTGTAATGACGACTATTAAGAAGGAATTTACCTCCCACCGCGTACCTGT
AGAGAAAAATATTCACGGAATTTGGATTGCAGGTTCACCGCCAGAAGGGACCGATGAATATATTAAATTG
TTCCTTCACACTTATCCAGAGTTTTCCTTCCTTTTTTGGGTGGATAAAACAGCTTATGGCGCAGCAAAAT
TCTCTTCTACTCTTAAACGAATTGCTTTTGATGCAGCAGTTAATTCCTTACGAGAGGCTACTCCAGAGCC
TGTCAAGCAGTTTGTTCAACGTTATGATAAGCTAAAAAAATCATACGATACTTCGCGAGATTTTGATGAA
AAACAGCGGTTATCTGAGCAGTTAGTTGAGCTATACGATAATTACAATAAATTTAGTAAGGAGATTCAAA
GTAACTTTGATGTTTTGCTATTGCATGAAATGATCACTATTCAAGATAGCTTTTTTAACTACTGCCAGCT
CAAAGGAGTAGGTGCAATAACAGATGAGACACGTATAGAGTATCTTGAGAAAGTTTTGAAAGTAAAAGAA
GAGGATCTATCTCACTATAAAGAAACTATCAAACGCAACAAAGAGTCTATTGAAAAACTTGTAAAAGAGA
TTAATGACTCTACTGGACGGGAGAGAGTAGTTATCAAAGACATCCGAGATTTGAAGTCTTTACAAGATCT
TACTAATAGCTACAACTATGAAACAGAAATGTTACTCCGATGGAATTATGCAGCGGCTACCGACCAGCTT
CGTATGTACATGCTTAAAGAATATGGGGGGATTTATACAGACCTAGATATTATGCCACAATACTCTCAGG
ATGTTTTGCAAAAAATTATGGATGTTGGTGGGAGTAGGTTTTTTGAGCATGATAAGTTACGGCGGACTCT
TTCTTTTGCAGCTCTAAAATTAGGAAGCGGTAAGCAAACAACTGTTTCCTTTGAAGAGGCTAAAAAAGCT
ATGACTCTCCCCACATTTACTCTTCAGGATAAGAGTCAAATTTCTGAAATTTTTAAATATCTAGAAACGG
AAACGCAAGCAAAAAAATCACTTTTCCAACCGATGGATGTTACCGTCGTCCGTGATTTCATGCCTATTTT
ACAACGTTATCATAAGTGGCAAACAGGATGGAATGTTCGGGGATTAAATGGTTTAATGATGGCTCATAAA
GATAGTGCTGTTGTCGATGCTGTTATTGCTCGCCAGCGAGCGGCCTATGATGAGATGCGTGCACTTCGTC
AGAATGTTGTTAGTGGAGAATTTTTTCGTTCTCTTGGAGATCTTGAACATGTAAATCGTGAAAAGAATAT
CGGCGGCTATTTAGCGAAAAATTATCTTGGGGGCAGTCTTTTTTTCGATTTTCGTCAAGATTCAGTGATT
CCAGGAGCTATTAGTACCTTAGGAATTTCAGGTCCCGATATCATCATGGATACCATGAGCGATTATTTTA
CAAATCTTGGACCAGTTGGAGAAGATTTTTTATATGAAGGGAAATTAGGAAAAGCAGCTTTCCTTGGAGC
TTATCAAGCACAAAAAACTCCGAAAGGAGAACTTACCTATGATTGGCTCCATCCTTTATCCATTGGAGCT
AATGACGTTACTCCTGCTGATGCAAGCACCTGGTGTGAAACTCGACAGCATTGTGCCGCTGAGCTTCTTT
TATCGGACTCTATATCCTCAGACGAACACCCTAAGGGTATTCGTCGAGAAAGAGTAAACCCGAATGATTT
CTCTAAATTATGGAGTAAGGAAGCTCAGGGAATCCTTTCTAGTGATTTCGCGGATCTTTTACCACGCTTT
AACTTACTGATAGAATCATCTGCGTTAGATATTCATACTTTATCGGCTCTTGATCGAGATATCCAACACC
TTTTTACAAAAGTTCAAAAAGACCCTGTAGCATCTGTAGCTGTATTTTCTTTACAGTTACAATTAGCTGA
AATGATTCGTGCGATTCCTTTCCCTATACGTAATCAGGTACATATCTTGCCTGAAGCACAAGCACATTTC
GAAGCAGATTGGAAGAAAGCAATCCAATTATATCTTCACAGTCATCCCCAAACAGAAGTGGTTATTTGGT
ACAGTTCTACTCATACTCAAATTGTTTTTGGAAAAGATTTGCTTGCTGTTGCAGAACGAGTTGCAGCAGC
AAAATCCTTAATGTCTGATCATGATCCATCACTTATAACCAGTTATCTAAAATATAAAACTCAATCTCAT
CTTGGGGTGCTTACTGAGTTTGATCAAGAAGATTTCTTTGAACTGATGGTAGATATTGCAGAGGAACCAG
AGCTTCATAAACAACTATTGAAAATAGAAGAACAAGTTAATTCAGGACTCTACTCTCATGTAGAGCATTC
TTTAGGAGAGTGGTTAAAATTATCCAAAGAAGAAAGAAAATCAAAGTTTCTTAAGATTTTAAAAGAAACG
TTTCAAGAGGAGGAAAGAGAAGACTCTCAACAACAACATAAAACATGGTTTGAAGAACTCTATGAGAAAA
GACATCAGGAGCGCGTAAAGGATCCAGCAAAAAAAATCCAAGAACTAATCACAGTTTTTCAAGAAAGTCA
GCGCGTTCAAGCTCAAGACATAGATACTTATTTTGCTCATAAACCGTTTTATCAAGATCTGATGAAAGAT
GGTTACGCTTTTGAGGACATTTCTGTGATTACGAAGTATCTTTTAGCGAGTGACGGTGTTTCAGGGATTA
TTACTACTGATCCTATATTTCCTCCTCCATCTAAACAGCTAATTGACGCTATGAAACAATCTTTGGGAGA
AGATTTTGGTGAGCTACACTATACGTTACAGATGGTCTATGATTGGTTATCTAAAGAGACTAACAGCGTA
ACTTCTGAACAGGCAAAACAAAAATTACCACAGAAACTACATGAAAAACTAGAAGGTTATACAACACATG
ATTTGCTTATTCCGCCTATTGATGGAAGCGTCAGCGCTTTAGGATTGCGATTTTCCACGGAGGAGGGGAA
GGTATCCGATCGCGTTTTAACAAGCATAGCACCTGGAGTTCCTAATTCTGCTAGCTATGCTATGACTAGT
TATTTGTATGGTTTGTTCTTAATTACGAAAGATATCCAAAGTGGTCGATTAACCCATGAAATACTAAAAG
AGAGATTACAAACCTATGGAGGCGCCTATTTTATCAATGAATCAAAAATTGATGTGCTACTTGCTTTATC
CCGGAAAAAAGCTCAGATATCCCTAATAGACGCCCATAAGGCTCTTACAGGTTTTTCTTCTTTTAGCGAA
GCATCTCTGGCTTTACTTACAGGAAGGATGCCCGGAACAAGCAGAGTTCTATCAAGAGAAGTAGAGTTTG
GACGACCTTCAGCTATCGTTATGGAAGGAGCAACAGCTATTCGTGCTCAGAGTTACGATGCTGTTGGATT
AAGGAAAGATTTTTTCTTGTTACCTCATACAGTTCCTTCCATACAGTCTATTGTTGAGCAAGCGAAATAT
ACAGTATTATCCTGGCCAGAATTTTATGAAAATCATGCGGATAAATGGAACGATCTTGCTAATCGTTTTG
GAGCTGAGGACCTTAGTGTTCATCCGCAAACATTTTTATATGACACAGAAGGTCGCTGTATGGGGCTTGC
TTTACTGTATATGTTAGCAGATGACTCTGTATCCTACAGATTATTACAACAAAATCTGATGACTTTAGCT
TCGTTATTTGATGAGCAAAACAGAAGAAATATCCCTTTAACTCCAGCTGATCAGAAATTTTTAAATAAGG
GGCTTTCTTTAATTGAGTGGTTACAGTTCAAAGGAAATCAACAACTTCAAACAGAGGGATTTTTCCATAC
CTTAGACTGGGATATCCCTCAATTAATGAAGCACTTTGCTTCTTCAACTGTAAAAAGTTGGTTAATTACT
ACGCCTGCGCATAGTCTTGTTCTTTCTTTAATGGGGAATTTTTTTAGAGTAACAGATCCAAACTATGGCC
ATACAGACTTTCCTTCATTAGAAGCTGCTATTACTTTTTTAGAAAGGATGGTTCAAGTATCCCCAGCTGT
TTTGGAGCGTTATGGATTTGATAAGGAAAAGTCTGTAACTAGCCAACTCAAAGTCCACTCTTTAGAGACT
TCAGAATTGCAAAATGCAGTTTTTGCTTCCTCAGATCTTGGCTTTACTAGTCGCTATTTTACGACAACGT
TAGAAGAGATGACCGTCAGAGGCCCTATTACGATGAATCAACGTCACACGGATTGGGCCACTCTTTATAA
GATTGGGGGGACTGTTCAAGGTAAACGTATTGATAGTAGAACACGGGAATCGGATTTAAATTTTTTAAAA
ATTAATGGGGATATTCTAGAGGAATTTTTAACAAGAACTGTTCTAGATAGTGACTTGGTGGAGTTAATAC
AATCTCTGCTCAAAACACATGGCCTTGAGCCAGGCACTACCCTTATTAGTCCAAGTTCTATTGTAGAGAC
CGCAATAGACCACGTCTCGCTATTACAAGCGGTGAAAACAAAAACCTCTCGAATGCACACTATTTTACAA
AGCCTAGGAGAAAGAATTTTTAAATTGTTTAAAAATTCTGGGGTACAAGACTCAGACAAAATTTCTATAG
ACAGAGTCCAACTAGTTGATGAATCTGATAGCGCAACGATAGACTTCACAGTGATTAAAGATAAACAGCG
TTCTCAGAAAAAAAGTATTACTGTTGGCATAGAAAGTTTAGCTGGATCCTTTAGAAAATTTAGCGCATCG
ATGCACGAAGTTATTGGAACTGGAGTGCTTGATTTAGACTTAGGAATGACTGTCGTCTCTTTAGTTCAGT
ATGTCCGTTTAGTAGAAGCAGGACAAGGAAAAGATGCTTTAGCAGTTGCTAATTTAGTGATGAATCTTAA
AATAGCTCTCGAGGTCTCTATAGGGAATGTAATTCAGGCTCTGGGTAAAAAACTGCTCACTCAGGAGGGG
CTTAATACTTTCCGTTTAGAAACAGAATTAGCTCGTCAACTACACAAAGTGGGAGCACGAGTTGGAGGAA
CTGTTGGTAAGACGTTAACCAGAGTAGCGCATGTATTAGAACTCCCCATATTAGAAAGTGCAATTGGAAC
ATGGAACCTCTATAATAGTGTGAACGAGTTATTACATGCTGATTCTTGGAGTGACCAGGTTGCTGCTAGA
GTACAAGTAGCTTTTGATTCAATCTCTTTAGGAATCACTATAGCTTCGGTTGTTTCTCCAGCACTTATGA
TGGCAGCAGGCCCCATTGCTGCCATAGGGATGGGGGCAGCATCCATTGCTCGTAATGTTGCCAGAAAAGA
AGAGCGGCACTCTCAATGGCTCAAGTACAAGAGTTTTCTTGATAATGGATCAAAACATACAGTTGCCGCA
TTCCCTCATAAAGGGCTTATAGATTTATCTGAGAATTTAGTACTTGGGAACATTGTGCTGAATTTAAGGG
TTTACCCACCTCTGCTTACAGGCGATCGCTCTTATAATGCAAATCGGTGGTTTGGGAATAAGCCTGGATG
GTCTGATTGGCAAGTTCGAGAAAGATTAGGTTATGCCTATCGAAGCAGTCCATCTTATGCGTTAGCCAGA
GGACACGCTAATAGTTTTTGGCCACTATCTATGCCTAGTATTGATAAAGGTGTATATCGCACAGTAATTC
TAGGATATGGCATTCAATATAAAGCGGTTACAGAAGTTGTCTACTTGTCTAATGAAATGGTTTGGAGAGA
AGCTGTGATGCAGTTTGACTCTCGATACTATGTAGAGCCATTAACAGCGGAAAAGGAGTGTGCAACAGTA
CTAGCAGGAGATACGCCACTATCAGTGATTCCTGTACGTTTATTAGAAGAGGAGTCTCTGGAACGCGAGA
AGAACGCGGCTTCTTATAAGAACTATCAGATCATTATTGAGGGAGGGAAAGGAGGACTTACTGTACAGAT
AGGAGGAGCAGGTTATTACAAGTTAACAGCACAGCCAGGAAAAGGGAATATCCTCTCTTTCCGAGCAATA
CCTGGATATCTTTCTGTAACGTTTGATCTTAGCCGATTGGAACAAGAGGTGCCTCTCACCAAACAAAATG
GAACAGCCTTAAAGATACTTAAAGTACGTCAAACTGGGTTTGATACTATTGTTGGATCTTCAGGAGGTAG
TGATAATTTAACAGGCAATCACAATACAAAATTTTATCTAAGCACCGGAGGAGGACATGTCACCTCAGGA
TCCGGTAAGAACTGGTATAACATTCCATCTTTAACACGTAGCCTTGGTTTTGATTTTACTTCTAATGCTA
TAGAGCATAAGGCTTCTGTAGAGATGCTTTTAGCAGATTTCTCTCCTGCTAATAATTTAAGTTTAATAGT
TGAACGATTTGCAAATGTTAGCATTCATGTTTTTAATTATAGCAATAAAACAGCCCCTTATACTGTGAAT
TTGAAAGACGGGGTGTCTTTACAAGCTTCTAAGCAAGGCTTTAGCGGTAGTTTATTAGAGGTGGCTTCCT
TTGATCAAACGCTATGGCAAAAGAGCTATCCAGAAGATAGAGGATTTGTAGAAGATATTTTATCTTGGTT
ATTAAAACTGCAATGGTCGTTAGCTTCTCGAGTAAGGATCTTTTTACAGGGAGGCACAGCACAATACGAA
ACAACGCAGAAAAGTCTTATTTATAGACCTGATCCACACTCAAGTATATCTATTCAGGCTACAGATTCTT
ATAATACACAAGTTTATGGTAACGTTGGCTGTTCTTATATTCTATTCTCTTCACCAGGTGTGACCGCCAA
AACACTCGATATTTTGTTATTCGAAGATTCGGGGTTACCACAAATTATTGACTTAAGCACACTTGTTCCA
ACTTCTATCCAAGGGTTCCTCTATCCAGGAGGGTTTATAAACTTTCAAGTCTCTTCTGCAAGATATGCAT
TTCCTTTTTCTGTCAGTTGGAGAAGGCATCATTACACTTTCCCTGCTCAAACGATTATTCAAGTTCTACC
TCGCTTGCGGTTTGAACTAGGGGATTGGTTTACAATTTTGCAAAAGAGTGTAGGAAAATGGGTAACATTG
TACCAACACGACATGATTATCCCTGAACGTATTGAAGGTGTTTTAAGTCTAAATAACACAGCGACATTAA
TGTCACACCACAATCAAACAACCCATATGCTTGGAGTAGAAAACAGGGGAGATTTGAGTCTAAAAGTTTT
AGGAATACTCCAATCTGGTCAAATTAAAGGATCTAAATCGGAAAATAGCGCGAGTGGTCATTTTTCTAAA
TTGTTATCTGTCCCAGCACATACGATCAAAAATCTTTCGTTTAAAGAGGAAGAGGGAACTAAGAGTAAAA
ATATTTTATTCTACAGTGTGCTGGAGGAAACATTCCTTAAAGCTACAGATAAACCTTTAACTATTATTAA
ACGTGATAAATGGTCTCTGTATGATGAAATCCAAGTATTTGCAACAACTCTTAATCTGCAAAATTTCTTG
CGTTATCATATTTCGGAAGAGACTCCAATGTTATCTAGACTGCTCATGTATGCGCAGAAACGCGTTTCCA
TCCAAAATAGAGATCTCGCATTGAAATTTTTCTATGTTCGAGAGCGAAGTGGTATTGGTGCAATTAGATT
GGTATTCAAGAACTTTTTCGAAGAGAGCTTGCAGGGGATATTACATGGAACCTTAGAGCGAGAAGTTAAA
CCTATGCTAGCTGAAAATCCAAATACACTAATTCATTCATCGTATCGGAATCATTTAGAACTCATCCTAG
GGGAGGAAACCTTAGATCTCGCAATTATCGTTCAAGAGTTTAGCTCTTCAAAAAATATTGTTGAGTTGCA
AAAAGATCTAGCAACCCATCGGCTTATTTATCCTCAAGGAAAAGAAGCCTTGTCTCTTGCAATCTATACC
CATACTTTCAGTAAAGAGAGCTTAGTCACATCTAAACAACGAAGCGAAGGGTTAAAATTCATCGATCCAT
CAATGCAGGAATATCGTTTACCTGAGACAACGATTCTCGAAAACTCTTATTATTTAGACCCTTCCACAGG
AGATTTGTATTTAACAAAGATAATTGCGCAACCTTCACAAAGTAGAGCATTTCTTATCAAATTTAAAAAA
TACAAACGCCACTGGCTAGATTTCCAAAAATTAGTTCTTTCTGGATCTCATTTAGAACTTATCGCATCTA
CTGGAACAGCTTTAACATTTGTAGGCCCCGAATTACGGCATTTAGAGATTGACTTCCCAGGCGCGTTAAA
TGGGACTATTGCCAAAGAACACATCTCATCTCGGGCAAGTATTGTAATCCCAACAAACAATCAGGTGACA
CACTATGACCCAAGAATAGCGAAGCAGCATTATAGCTCTATCAATTATATGCTGTGGGATCTGCGTGATC
GTGCGACACTATCTAAGCGAGCAAAAACTTTAGATAGCTATTTGTTAGAAGTTTGTATGAACTTAGACTC
TTCTAATCCAACGTGGAATATTCCAAATGATGTGTTGAACTACGCGGTAGGATATTATCGAACAATCCTT
CCTAGCTGGGTAAAAAATAAAATCCGTGTAGGTTCCTTAATTAAAGTAGAAGCTGATACTGCAAAAACTT
TGAGTTTCTCACTAATTACGACACAAAATGATCTATTCCAACCTGTAGTGGATAAAGGATTCTATATTTA
CTACAGCGTTTCGAAACTAGCCAATCATGTTAAACTGCGAAATGTTACTGGAGATATGTTGCTTGATATC
GATAAGGAAACCACATTTATTGTTCGAGGAGTAGATGAATCTGATTATGATAAGAAACAAATCTATGTTG
TTTTAGATCTGACAACAGAAGAAGAACGTAAATTGCGAACGGATAAGAACATCATTATTATTCCTGGAGG
AGAACGATTGAACCGATG
  • Protein Sequence : Show Sequence 
    >gi|15835057|ref|NP_296816.1| adherence factor [Chlamydia muridarum Nigg]
MEQALCTMTLSNTASSTVSPKSTQLNHTLSNQTHPVAQSNRNIDFQIYDEQIHNNHSTEDVVAIGRRIQQ
EYANLTTSKQVNFSASPSNHTGNWKISLLYNLSMLVANLFPTTIQPVQPQKVIFKNQDSSKETQLTKSSK
VVSSRSSLKEIFSKKSPPLRKSFSSSKISRAIPRRKKRSNDDPVQATNTYDLTAENILEKLSLTQEQQIK
HDNLISNLKEAINRYSDLNRKNSRKGQSLLVRQAKILDEILSQTKSTEERASNSVMTTIKKEFTSHRVPV
EKNIHGIWIAGSPPEGTDEYIKLFLHTYPEFSFLFWVDKTAYGAAKFSSTLKRIAFDAAVNSLREATPEP
VKQFVQRYDKLKKSYDTSRDFDEKQRLSEQLVELYDNYNKFSKEIQSNFDVLLLHEMITIQDSFFNYCQL
KGVGAITDETRIEYLEKVLKVKEEDLSHYKETIKRNKESIEKLVKEINDSTGRERVVIKDIRDLKSLQDL
TNSYNYETEMLLRWNYAAATDQLRMYMLKEYGGIYTDLDIMPQYSQDVLQKIMDVGGSRFFEHDKLRRTL
SFAALKLGSGKQTTVSFEEAKKAMTLPTFTLQDKSQISEIFKYLETETQAKKSLFQPMDVTVVRDFMPIL
QRYHKWQTGWNVRGLNGLMMAHKDSAVVDAVIARQRAAYDEMRALRQNVVSGEFFRSLGDLEHVNREKNI
GGYLAKNYLGGSLFFDFRQDSVIPGAISTLGISGPDIIMDTMSDYFTNLGPVGEDFLYEGKLGKAAFLGA
YQAQKTPKGELTYDWLHPLSIGANDVTPADASTWCETRQHCAAELLLSDSISSDEHPKGIRRERVNPNDF
SKLWSKEAQGILSSDFADLLPRFNLLIESSALDIHTLSALDRDIQHLFTKVQKDPVASVAVFSLQLQLAE
MIRAIPFPIRNQVHILPEAQAHFEADWKKAIQLYLHSHPQTEVVIWYSSTHTQIVFGKDLLAVAERVAAA
KSLMSDHDPSLITSYLKYKTQSHLGVLTEFDQEDFFELMVDIAEEPELHKQLLKIEEQVNSGLYSHVEHS
LGEWLKLSKEERKSKFLKILKETFQEEEREDSQQQHKTWFEELYEKRHQERVKDPAKKIQELITVFQESQ
RVQAQDIDTYFAHKPFYQDLMKDGYAFEDISVITKYLLASDGVSGIITTDPIFPPPSKQLIDAMKQSLGE
DFGELHYTLQMVYDWLSKETNSVTSEQAKQKLPQKLHEKLEGYTTHDLLIPPIDGSVSALGLRFSTEEGK
VSDRVLTSIAPGVPNSASYAMTSYLYGLFLITKDIQSGRLTHEILKERLQTYGGAYFINESKIDVLLALS
RKKAQISLIDAHKALTGFSSFSEASLALLTGRMPGTSRVLSREVEFGRPSAIVMEGATAIRAQSYDAVGL
RKDFFLLPHTVPSIQSIVEQAKYTVLSWPEFYENHADKWNDLANRFGAEDLSVHPQTFLYDTEGRCMGLA
LLYMLADDSVSYRLLQQNLMTLASLFDEQNRRNIPLTPADQKFLNKGLSLIEWLQFKGNQQLQTEGFFHT
LDWDIPQLMKHFASSTVKSWLITTPAHSLVLSLMGNFFRVTDPNYGHTDFPSLEAAITFLERMVQVSPAV
LERYGFDKEKSVTSQLKVHSLETSELQNAVFASSDLGFTSRYFTTTLEEMTVRGPITMNQRHTDWATLYK
IGGTVQGKRIDSRTRESDLNFLKINGDILEEFLTRTVLDSDLVELIQSLLKTHGLEPGTTLISPSSIVET
AIDHVSLLQAVKTKTSRMHTILQSLGERIFKLFKNSGVQDSDKISIDRVQLVDESDSATIDFTVIKDKQR
SQKKSITVGIESLAGSFRKFSASMHEVIGTGVLDLDLGMTVVSLVQYVRLVEAGQGKDALAVANLVMNLK
IALEVSIGNVIQALGKKLLTQEGLNTFRLETELARQLHKVGARVGGTVGKTLTRVAHVLELPILESAIGT
WNLYNSVNELLHADSWSDQVAARVQVAFDSISLGITIASVVSPALMMAAGPIAAIGMGAASIARNVARKE
ERHSQWLKYKSFLDNGSKHTVAAFPHKGLIDLSENLVLGNIVLNLRVYPPLLTGDRSYNANRWFGNKPGW
SDWQVRERLGYAYRSSPSYALARGHANSFWPLSMPSIDKGVYRTVILGYGIQYKAVTEVVYLSNEMVWRE
AVMQFDSRYYVEPLTAEKECATVLAGDTPLSVIPVRLLEEESLEREKNAASYKNYQIIIEGGKGGLTVQI
GGAGYYKLTAQPGKGNILSFRAIPGYLSVTFDLSRLEQEVPLTKQNGTALKILKVRQTGFDTIVGSSGGS
DNLTGNHNTKFYLSTGGGHVTSGSGKNWYNIPSLTRSLGFDFTSNAIEHKASVEMLLADFSPANNLSLIV
ERFANVSIHVFNYSNKTAPYTVNLKDGVSLQASKQGFSGSLLEVASFDQTLWQKSYPEDRGFVEDILSWL
LKLQWSLASRVRIFLQGGTAQYETTQKSLIYRPDPHSSISIQATDSYNTQVYGNVGCSYILFSSPGVTAK
TLDILLFEDSGLPQIIDLSTLVPTSIQGFLYPGGFINFQVSSARYAFPFSVSWRRHHYTFPAQTIIQVLP
RLRFELGDWFTILQKSVGKWVTLYQHDMIIPERIEGVLSLNNTATLMSHHNQTTHMLGVENRGDLSLKVL
GILQSGQIKGSKSENSASGHFSKLLSVPAHTIKNLSFKEEEGTKSKNILFYSVLEETFLKATDKPLTIIK
RDKWSLYDEIQVFATTLNLQNFLRYHISEETPMLSRLLMYAQKRVSIQNRDLALKFFYVRERSGIGAIRL
VFKNFFEESLQGILHGTLEREVKPMLAENPNTLIHSSYRNHLELILGEETLDLAIIVQEFSSSKNIVELQ
KDLATHRLIYPQGKEALSLAIYTHTFSKESLVTSKQRSEGLKFIDPSMQEYRLPETTILENSYYLDPSTG
DLYLTKIIAQPSQSRAFLIKFKKYKRHWLDFQKLVLSGSHLELIASTGTALTFVGPELRHLEIDFPGALN
GTIAKEHISSRASIVIPTNNQVTHYDPRIAKQHYSSINYMLWDLRDRATLSKRAKTLDSYLLEVCMNLDS
SNPTWNIPNDVLNYAVGYYRTILPSWVKNKIRVGSLIKVEADTAKTLSFSLITTQNDLFQPVVDKGFYIY
YSVSKLANHVKLRNVTGDMLLDIDKETTFIVRGVDESDYDKKQIYVVLDLTTEEERKLRTDKNIIIIPGG
ERLNR
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0439 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
  • Related Vaccine(s): C. muridarum DNA vaccine encoding TC0439
6. TC0512 outer membrane protein, putative
  • Gene Name : TC0512 outer membrane protein, putative
  • Sequence Strain (Speices/Organism) : Chlamydia muridarum Nigg
  • VO ID : VO_0010885
  • NCBI Gene ID : 1245872
  • NCBI Protein GI : 15835130
  • Locus Tag : TC0512
  • Genbank Accession : AE002160
  • Protein Accession : NP_296889
  • Taxonomy ID : 243161
  • Gene Starting Position : 618235
  • Gene Ending Position : 620613
  • Gene Strand (Orientation) : +
  • Protein Name : outer membrane protein, putative
  • Protein pI : 9.51
  • Protein Weight : 84655.32
  • Protein Length : 792
  • Protein Note : similar to GB:X75201, and PID:435005; identified by sequence similarity; putative
  • DNA Sequence : Show Sequence 
    >gi|29337300:618235-620613 Chlamydia muridarum Nigg, complete genome
AATGCTTGGAATACGAAAAAAAACGATTCTGCAACTCGCTATTTTACTGTTGCTCCCCTTTTCACAAAAC
GCCTTTGGAACATCTCCTGAAGGACGTATGGTGGTACAATCCATCACTATTGAGACTCGAGGAGAAAATG
CTCAAAATAAGCGGGCTATTCCCAAAATAAAGACAAAGCAAGGGACGCTGTTCTCTCAAACTGATTTTGA
CGAAGATCTAAGAACTCTCTCGAAAGATTTCGACCGCATTGATCCTATTGTAGAGTTTCGCAATGGGCAA
GCTGTTATTTCCCTTATTCTGACAGCAAAACCCGTTATCCGACACATTAACATCTCAGGGAATGAAGCAA
TTCCTACTCATAAAATTTTAAAAACCCTAGAGCTTTATAAAAACGATCTTTTTGATCGAGAGGTTTTTTT
CAAAAATTTTGATGCTTTAAGAACCCTTTACTTAAAACGCGGATACTACGATTCTCAATTGTCCTATTCT
CACGATCATAATGAGCAAGAAGGTTATATTGATATCTCAATCCAAATTAAAGAAGGGCCCTACGGACGGA
TAAAAAAATTAACAATTTCAGGTATTACTCGTACAGAAGCCTCTGATTTGGGAGACATAGTTTTAACCAA
ACAGTACTCTACAACAACAAGTTGGTTCACTGGAGCAGGGGTATACCATCCAGACATGGTGGACCAGGAT
CTCTTTGCCATAACAAACTATTTTCAAAATAAAGGTTATGCAGACGCAAAAGTCACAAAAGAAGTCTCCA
CAGATGCTAAAGGAAACCTCTCTCTTGTTATCAACGTAGATAAAGGGCCCCTGTATACTTTAGGTCACGT
ACATATAGAAGGTTTCACAGCTTTATCCAAGCGGTTACTTGATAAACAACTGTTAGTGGGCCCCAATTCC
TTATATTGCCCAGAAAAAGTTTGGGCTGGAGCTCAAAAAATCCGTAATGCCTACGCTAGATACGGATATG
TAAATACCAATGTAGACGTATCTTTTGCTGTTCATCCAACGCTCCCAGTTTATGATGTTACTTACAGGGT
TAGCGAAGGAGCTCCTTACAAAATCGGACTAATCAAAATCAAAGGGAACACACACACCAAACATGACGTA
ATTTTACATGAGACAAGTCTGTTCCCTGGAGATACTTTTGATAGATTAAAACTAGAAGATACGGAAACTC
GATTACGTAACACAGGCTACTTTAAAAGTGTGAGTGTCTACACGGTACGCTCACAATTAGATCCTTTGGA
CTCAAATAACCTTTATAGAGACGTTTTCATTGAAGTCAAAGAAACCGAAACAGGTAACCTAGGACTGTTC
TTAGGATTCAGTTCTATAGACCATCTGTTCGGAGGAGCAGAAATTTCAGAAAGCAATTTTGATTTATTTG
GCGCCCGACACTTATTCAAAAAAGGGTTTAAATCCTTAAGAGGTGGTGGGGAATATCTTTTCTTAAAAGC
CAATTTAGGAGACAAAGTTACTGATTATACTGTTAAATGGACAAAACCTCATTTCTTAAACACCCCATGG
ATCCTAGGAGTAGAATTAGATAAATCTATTAATAAGGCTTTATCAAAAGATTATTCCGTTGATACCTATG
GAGGAAACATCAGCACGACATACATTCTTAACGACAAACTGAAATATGGGATGTATTACCGCGGGAGCCA
AACGAGCCTAAGTTTAAGAAAAAAAACAGAAAATCCTAACAAACCTGGACCAGATCTAGATAGTAACAAG
GGATTTGTTTCCGCGGCAGGGCTTAACGTTCTTTACGATTCTATTGACAATCCTAGAAAACCTACTATGG
GTATTCGGAGCTCTTTGAACTTTGAGTTGTCTGGATTAGGAGGAACCTATCAGTTTACAAAATTAACAGC
CAGCGGCTCTATCTATCGCCTGCTAACTAAAAAAGGTGTTTTGAAAATTCGTGGAGAAGCTAAGTTTATT
AAACCTTTCGGTACAACCACAGCCCAAGGAATTCCTGTTAGTGAGCGCTTCTTCTTAGGAGGAGAATCTA
CCGTTCGTGGATATAAACCATTTATTATTGGGCCTAAATTTTCTCCAACAGAGCCTCAAGGGGGCTTATC
TTCCTTATTGCTAACAGAAGAGTTTCAATATCCTCTAATTTCTCAACCCAGCATCAATGCATTCGTTTTT
CTAGATTCGGGCTTTATTGGCACGGAAGAGTACACTATTCGACTCAAAAATCTCTGTAGCAGTGCCGGAT
TTGGGTTGCGTTTTGATATGATGAATAACGTGCCCATTATGTTAGGTTGGGGTTGGCCTTTCCGCCCAAC
AGAAATTCTTAATAATGAAAAAATTGATGTATCTCAAAGATTCTTCTTCGCTTTGGGAGGAGTGTTCTA
  • Protein Sequence : Show Sequence 
    >gi|15835130|ref|NP_296889.1| outer membrane protein, putative [Chlamydia muridarum Nigg]
MLGIRKKTILQLAILLLLPFSQNAFGTSPEGRMVVQSITIETRGENAQNKRAIPKIKTKQGTLFSQTDFD
EDLRTLSKDFDRIDPIVEFRNGQAVISLILTAKPVIRHINISGNEAIPTHKILKTLELYKNDLFDREVFF
KNFDALRTLYLKRGYYDSQLSYSHDHNEQEGYIDISIQIKEGPYGRIKKLTISGITRTEASDLGDIVLTK
QYSTTTSWFTGAGVYHPDMVDQDLFAITNYFQNKGYADAKVTKEVSTDAKGNLSLVINVDKGPLYTLGHV
HIEGFTALSKRLLDKQLLVGPNSLYCPEKVWAGAQKIRNAYARYGYVNTNVDVSFAVHPTLPVYDVTYRV
SEGAPYKIGLIKIKGNTHTKHDVILHETSLFPGDTFDRLKLEDTETRLRNTGYFKSVSVYTVRSQLDPLD
SNNLYRDVFIEVKETETGNLGLFLGFSSIDHLFGGAEISESNFDLFGARHLFKKGFKSLRGGGEYLFLKA
NLGDKVTDYTVKWTKPHFLNTPWILGVELDKSINKALSKDYSVDTYGGNISTTYILNDKLKYGMYYRGSQ
TSLSLRKKTENPNKPGPDLDSNKGFVSAAGLNVLYDSIDNPRKPTMGIRSSLNFELSGLGGTYQFTKLTA
SGSIYRLLTKKGVLKIRGEAKFIKPFGTTTAQGIPVSERFFLGGESTVRGYKPFIIGPKFSPTEPQGGLS
SLLLTEEFQYPLISQPSINAFVFLDSGFIGTEEYTIRLKNLCSSAGFGLRFDMMNNVPIMLGWGWPFRPT
EILNNEKIDVSQRFFFALGGVF
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0512 showed significant levels of protection when compared to negative control animals. At 9 days following challenge TC0512 showed a 73% reduction in the number of recoverable Chlamydia compared with vector only immunized controls (McNeilly et al., 2007).
  • Related Vaccine(s): C. muridarum DNA vaccine encoding TC0512
7. TC0757 hypothetical protein
  • Gene Name : TC0757 hypothetical protein
  • Sequence Strain (Speices/Organism) : Chlamydia muridarum Nigg
  • VO ID : VO_0010886
  • NCBI Gene ID : 1246122
  • NCBI Protein GI : 15835371
  • Locus Tag : TC0757
  • Genbank Accession : NZ_ACOV01000003
  • Protein Accession : NP_297130
  • Taxonomy ID : 243161
  • Gene Starting Position : 898853
  • Gene Ending Position : 899647
  • Gene Strand (Orientation) : +
  • Protein Name : hypothetical protein
  • Protein pI : 5.02
  • Protein Weight : 27979.63
  • Protein Length : 264
  • Protein Note : identified by match to TIGR protein family HMM TIGR01607
  • DNA Sequence : Show Sequence 
    >gi|29337300:898853-899647 Chlamydia muridarum Nigg, complete genome
CATGCGCTGTTCTGCTTATTGCTCTGCTTCGTCCTATCATCTACACGTTCTTTTTCATCTTTTAAAGGCA
CGCTACCCTTCTGTCTTATCCCGAGAATACGTGCTCATTTCCTCTGAAGAGCTGAATGAAAGCGACAAAG
CCGCTGTCTTTTTCCCATTTGGAGTTAGTGTGTTTTGGGGATGGGAAGAAGCTGAAGAACTTCAAGTGAT
CCAAGCTATCACCTCAGCATCAGTGGATCCTCTCCCTAACCCAGAAATTGATAGTTATGACTTCCATTAT
GGAGAGAAACTACAGATCCGTCGCGATAGACTTGTTCTCACCAACTCTAATTTAAACACAAAACTTGCTA
TTTCCTTTGGGTTAGCCCAATCTATCAAACTTACCGTCTTTGAAGAAACGATTTACAAAACAGTTGAAAA
CTCTAAATCTTTGCCTCAAGAACTTGCCTCTAAAGGGAAAATTTCTCTATCCCGTAAAACGATCGCAAAA
AAGATTGGAGAGCTTTTCCTGGATAAAGCTTCCGTAAACCTACACTCTGACATTCTCGATGAGCCCGACT
TTTTCTGGGAACATCCAGAAACCCAACCTTTTTATATTGATGTGTTGACCTGCCTAGATGTAAACGCGCG
AGTAAATGTATTAAATCACAGACTTGCTATCCTAGGAGATGTTCTCGAGATTTTAAATGACCAACTTAAT
CATCAGCATTCTTCAGCTCTAGAGTGGACCGTGATCTGGTTAATTGCACTAGAGGTATTGGTCACCTTAC
TTAAAGACGTATTCAACATCATCTA
  • Protein Sequence : Show Sequence 
    >gi|15835371|ref|NP_297130.1| hypothetical protein TC0757 [Chlamydia muridarum Nigg]
MRCSAYCSASSYHLHVLFHLLKARYPSVLSREYVLISSEELNESDKAAVFFPFGVSVFWGWEEAEELQVI
QAITSASVDPLPNPEIDSYDFHYGEKLQIRRDRLVLTNSNLNTKLAISFGLAQSIKLTVFEETIYKTVEN
SKSLPQELASKGKISLSRKTIAKKIGELFLDKASVNLHSDILDEPDFFWEHPETQPFYIDVLTCLDVNAR
VNVLNHRLAILGDVLEILNDQLNHQHSSALEWTVIWLIALEVLVTLLKDVFNII
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0757 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
  • Related Vaccine(s): C. muridarum DNA vaccine encoding TC0757
8. TC0767 hypothetical protein
  • Gene Name : TC0767 hypothetical protein
  • Sequence Strain (Speices/Organism) : Chlamydia muridarum Nigg
  • VO ID : VO_0010888
  • NCBI Gene ID : 1246132
  • NCBI Protein GI : 15835381
  • Locus Tag : TC0767
  • Genbank Accession : AE002160
  • Protein Accession : NP_297140
  • Taxonomy ID : 243161
  • Gene Starting Position : 910619
  • Gene Ending Position : 911350
  • Gene Strand (Orientation) : -
  • Protein Name : hypothetical protein
  • Protein pI : 9.14
  • Protein Weight : 26828.18
  • Protein Length : 243
  • DNA Sequence : Show Sequence 
    >gi|29337300:910619-911350 Chlamydia muridarum Nigg, complete genome
ATTAATGCAAAATAAGGAATCGTTTTAATTGGCGGAAAGAGAGCTGATAAAGAGATGGGATCCCTCGACT
ATCTAAAGCCATATCAATACGAGGAATGCTATGCTGTGATTGCATCGAGTAAAAAAAACTCGCAGCATCC
CCTAACACAAAAGAATAGCGGTAAACCTCCTGAACAAAATGTTGACTCACCAAATCACTTTTTCCAAAAG
GATAAAAAAAGACTTCCACAGGAGCCTGTATAGCCTCTTCTAGTAAAATTTTAGACAATGAAATTTCAGT
ATGTAAATAAGGAGGGGAGTTTTTAAGGTTTCTAATAGCAAACCCTGAAGAGGCAAATCGAATAATCGAA
CTTTGAGCCATATGACAAAGTTCTTTAACCGAACAAAAGGGCTGATAATAAGAAAATATTTCGTCTTGAA
AAGCTAGAAAATCCGATGGCGAAATTCGCATATCAATGGGAAGATTTTCACTTTCTAAACGGGATACGTA
TCTCCAAGCAACTCCAACAACCGCAGGAATCTGGAATTTCTGAAGAAAAGGAAAGACATGCTTGTAAAAA
TCAACAGAAGCATAATCGAATGTCAGTATGATTGCTTTCCTATTAGGGAGAGGATCTCCGGGCAGAATAA
ACGAATAATGCTGCTTGAGAGAGTGCAAAAAATCTAAAAACGATCTGAGGGCATAAGGAAACTTAGAAAA
AGCAACCTGTCGATAGGCTAAGACGCGGAGCA
  • Protein Sequence : Show Sequence 
    >gi|15835381|ref|NP_297140.1| hypothetical protein TC0767 [Chlamydia muridarum Nigg]
MLRVLAYRQVAFSKFPYALRSFLDFLHSLKQHYSFILPGDPLPNRKAIILTFDYASVDFYKHVFPFLQKF
QIPAVVGVAWRYVSRLESENLPIDMRISPSDFLAFQDEIFSYYQPFCSVKELCHMAQSSIIRFASSGFAI
RNLKNSPPYLHTEISLSKILLEEAIQAPVEVFFYPFGKSDLVSQHFVQEVYRYSFVLGDAASFFYSMQSQ
HSIPRIDMALDSRGIPSLYQLSFRQLKRFLILH
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
  • Related Vaccine(s): C. muridarum DNA vaccine encoding TC0767/768
9. TC0768 hypothetical protein
  • Gene Name : TC0768 hypothetical protein
  • Sequence Strain (Speices/Organism) : Chlamydia muridarum Nigg
  • VO ID : VO_0010889
  • NCBI Gene ID : 1246133
  • NCBI Protein GI : 15835382
  • Locus Tag : TC0768
  • Genbank Accession : AE002160
  • Protein Accession : NP_297141
  • Taxonomy ID : 243161
  • Gene Starting Position : 911335
  • Gene Ending Position : 911496
  • Gene Strand (Orientation) : -
  • Protein Name : hypothetical protein
  • Protein pI : 3.69
  • Protein Weight : 5224.4
  • Protein Length : 53
  • DNA Sequence : Show Sequence 
    >gi|29337300:911335-911496 Chlamydia muridarum Nigg, complete genome
GCTAAGACGCGGAGCATGGATCAACTGAAGGAATTGCAAAAGGGTCCTCTGGATAAATGACTACCTGTCC
GGAAATGATCGTTAGATTACGCTCTTTTCCCTCAGGAGTAAGCTCTGCTGTTTCCGTAGAACAGACTTCA
GAAGTAGGTAAGGAGATCGGCA
  • Protein Sequence : Show Sequence 
    >gi|15835382|ref|NP_297141.1| hypothetical protein TC0768 [Chlamydia muridarum Nigg]
MPISLPTSEVCSTETAELTPEGKERNLTIISGQVVIYPEDPFAIPSVDPCSAS
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
  • Related Vaccine(s): C. muridarum DNA vaccine encoding TC0767/768
III. Vaccine Information
1. C. muridarum DNA vaccine encoding TC0439
a. Vaccine Ontology ID:
VO_0011533
b. Type:
DNA vaccine
c. Status:
Research
d. Antigen
C. muridarum TC0439 adherence factor
e. Gene Engineering of TC0439 adherence factor
  • Type: DNA vaccine construction
  • Description: The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
  • Detailed Gene Information: Click here.
f. Vector:
pCI30
g. Immunization Route
Subcutaneous
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 104 live C. muridarum elementary bodies (McNeilly et al., 2007).
  • Challenge Protocol: All animals were challenged intra-vaginally with 5 × 104 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007).
  • Efficacy: The groups of female BALB/c mice immunized intra-abdominally with TC0439 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
2. C. muridarum DNA vaccine encoding TC0512
a. Vaccine Ontology ID:
VO_0011534
b. Type:
DNA vaccine
c. Status:
Research
d. Antigen
C. muridarum TC0512 outer membrane protein, putative
e. Gene Engineering of TC0512 outer membrane protein, putative
  • Type: DNA vaccine construction
  • Description: The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
  • Detailed Gene Information: Click here.
f. Adjuvant:
pCI30
g. Immunization Route
Subcutaneous injection
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 104 live C. muridarum elementary bodies (McNeilly et al., 2007).
  • Challenge Protocol: All animals were challenged intra-vaginally with 5 × 104 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007).
  • Efficacy: The groups of female BALB/c mice immunized intra-abdominally with TC0512 showed significant levels of protection when compared to negative control animals. At 9 days following challenge TC0512 showed a 73% reduction in the number of recoverable Chlamydia compared with vector only immunized controls (McNeilly et al., 2007).
3. C. muridarum DNA vaccine encoding TC0757
a. Vaccine Ontology ID:
VO_0011522
b. Type:
DNA vaccine
c. Status:
Research
d. Antigen
C. muridarum TC0757
e. Gene Engineering of TC0757 hypothetical protein
  • Type: DNA vaccine construction
  • Description: The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
  • Detailed Gene Information: Click here.
f. Vector:
pCI30
g. Immunization Route
Subcutaneous injection
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 104 live C. muridarum elementary bodies (McNeilly et al., 2007).
  • Challenge Protocol: All animals were challenged intra-vaginally with 5 × 104 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007).
  • Efficacy: The groups of female BALB/c mice immunized intra-abdominally with TC0757 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
4. C. muridarum DNA vaccine encoding TC0767/768
a. Vaccine Ontology ID:
VO_0011524
b. Type:
DNA vaccine
c. Status:
Research
d. Antigen
C. muridarum TC0767/768
e. Gene Engineering of TC0767 hypothetical protein
  • Type: DNA vaccine construction
  • Description: The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
  • Detailed Gene Information: Click here.
f. Gene Engineering of TC0768 hypothetical protein
  • Type: DNA vaccine construction
  • Description: The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007).
  • Detailed Gene Information: Click here.
g. Vector:
pCI30
h. Immunization Route
intra-abdominal injection
i. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 104 live C. muridarum elementary bodies (McNeilly et al., 2007).
  • Challenge Protocol: All animals were challenged intra-vaginally with 5 × 104 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007).
  • Efficacy: The groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007).
5. C. muridarum MOMP protein vaccine
a. Vaccine Ontology ID:
VO_0011453
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
C. muridarum major outer membrane protein MOMP
e. Gene Engineering of MOMP
  • Type: Recombinant protein preparation
  • Description: Recombinant maltose binding protein-MOMP (MBP-MOMP) was cloned into the bacterial expression vector pMAL-c2, and used to transform Escherichia coli (DH5α[pMMM3]). The MBP-MOMP was purified by passage through a PD-10 column (Amersham Biosciences, NSW, Australia), followed by a Sephadex G-75 column (Sigma–Aldrich) (Skelding et al., 2006).
  • Detailed Gene Information: Click here.
f. Adjuvant:
Cholera toxin and CpG oligodeoxynucleotide
g. Immunization Route
Intranasal immunization
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Groups of five mice were immunized on days 0, 7, 14, and 35 via either the IN or TCI route. One group of five mice were used as a control, receiving no immunization solution. Mice immunized intranasally were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia), and 10 μl of immunization solution containing 100 μg MOMP, admixed with 10 μg CpG-ODN 1826 (5′-TCC ATG ACG TTC CTG ACG TT-3′) (Geneworks, SA, Australia), and 10 μg cholera toxin (Sigma–Aldrich) was gently pipetted onto each nare (5 μl per nare) (Skelding et al., 2006).
  • Challenge Protocol: Five days after the final boost, mice were infected intranasally with C. muridarum. Mice were anaesthetised with 4% isoflurane and 103 IFUs were gently pipetted onto the nares. Mice were weighed daily for 10 days then sacrificed 10 days post-infection via a sodium pentobarbital overdose (Nembutal, Abbott Australasia). The lungs from the mice were either perfused and fixed for histopathology or collected for determination of C. muridarum levels (Skelding et al., 2006).
  • Efficacy: Both intranasal and transcutaneous immunization protected mice against respiratory challenge with Chlamydia (Skelding et al., 2006).
6. C. muridarum PmpE/F-2 protein vaccine
a. Vaccine Ontology ID:
VO_0011456
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
C. muridarum polymorphic membrane protein E/F family protein
e. Gene Engineering of PmpE/F-2
  • Type: Recombinant protein preparation
  • Description: The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: pmpE/F-2 and gap DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pET32a (Novagen) for pmpE/F-2 and gap after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques. Only the first half of the gene (25–575) was cloned into the vector for expression (Yu et al., 2009).
  • Detailed Gene Information: Click here.
f. Adjuvant:
Incomplete Freunds Adjuvant (Sigma)
g. Immunization Route
Intravenous injection (i.v.)
h. Mouse Response
  • Host Strain: C57BL/6
  • Vaccination Protocol: Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 106 DCs transfected with Chlamydia protein PmpE/F-225–575 in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009).
  • Challenge Protocol: Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009).
  • Efficacy: PmpE/F-2(25-575) immunized mice exhibited significant resistance to challenge infection. In addition, pmpE/F-2 was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009).
7. C. muridarum PmpG-1 protein vaccine
a. Vaccine Ontology ID:
VO_0011458
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
C. muridarum polymorphic membrane protein G family protein
e. Gene Engineering of PmpG-1
  • Type: Recombinant protein preparation
  • Description: The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: pmpG-1 and gap DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pET32a (Novagen) for pmpG-1 and gap after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques. Only the first half of the gene (25–500) was cloned into the vector for expression (Yu et al., 2009).
  • Detailed Gene Information: Click here.
f. Adjuvant:
Incomplete Freunds Adjuvant (Sigma)
g. Immunization Route
Intravenous injection (i.v.)
h. Mouse Response
  • Host Strain: C57BL/6
  • Vaccination Protocol: Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 106 DCs transfected with Chlamydia protein PmpG-1 25–500 in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009).
  • Challenge Protocol: Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009).
  • Efficacy: Overall, vaccination with DCs transfected with PmpG-1(25-500) exhibited the greatest degree of protective immunity among the four Chlamydia Ags tested (Yu et al., 2009).
8. C. muridarum RplF protein vaccine
a. Vaccine Ontology ID:
VO_0011437
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
C. muridarum rplF
e. Gene Engineering of RplF
  • Type: Recombinant protein preparation
  • Description: The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: rplF DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pGEX-6P-3 (GE Healthcare) after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques (Yu et al., 2009).
  • Detailed Gene Information: Click here.
f. Adjuvant:
Incomplete Freunds Adjuvant (Sigma)
g. Immunization Route
Intravenous injection (i.v.)
h. Mouse Response
  • Host Strain: C57BL/6
  • Vaccination Protocol: Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 106 DCs transfected with Chlamydia protein RplF in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009).
  • Challenge Protocol: Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009).
  • Efficacy: RplF was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009).
IV. References
1. Faludi et al., 2009: Faludi I, Burian K, Csanadi A, Miczak A, Lu X, Kakkar VV, Gonczol E, Endresz V. Adjuvant modulation of the immune response of mice against the LcrE protein of Chlamydophila pneumoniae. International journal of medical microbiology : IJMM. 2009; 299(7); 520-528. [PubMed: 19451031].
2. McNeilly et al., 2007: McNeilly CL, Beagley KW, Moore RJ, Haring V, Timms P, Hafner LM. Expression library immunization confers partial protection against Chlamydia muridarum genital infection. Vaccine. 2007; 25(14); 2643-2655. [PubMed: 17239501].
3. Skelding et al., 2006: Skelding KA, Hickey DK, Horvat JC, Bao S, Roberts KG, Finnie JM, Hansbro PM, Beagley KW. Comparison of intranasal and transcutaneous immunization for induction of protective immunity against Chlamydia muridarum respiratory tract infection. Vaccine. 2006; 24(3); 355-366. [PubMed: 16153755].
4. Wiki: Chlamydia muridarum: Chlamydia muridarum [http://en.wikipedia.org/wiki/Chlamydia_muridarum]
5. Yu et al., 2009: Yu H, Jiang X, Shen C, Karunakaran KP, Brunham RC. Novel Chlamydia muridarum T cell antigens induce protective immunity against lung and genital tract infection in murine models. Journal of immunology (Baltimore, Md. : 1950). 2009; 182(3); 1602-1608. [PubMed: 19155509].