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Staphylococcus aureus

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Disease
    3. Introduction
    4. Microbial Pathogenesis
    5. Host Ranges and Animal Models
    6. Host Protective Immunity
  2. Vaccine Related Pathogen Genes
    1. ClfA (Protective antigen)
    2. clfA (Protective antigen)
    3. clfB (Protective antigen)
    4. cna (Protective antigen)
    5. Csa1A (Protective antigen)
    6. efb (Protective antigen)
    7. entA_1 (Protective antigen)
    8. esxA (Protective antigen)
    9. esxB (Protective antigen)
    10. Fhud2 (Protective antigen)
    11. fnbA (Protective antigen)
    12. FnbpA (Protective antigen)
    13. gapC (Protective antigen)
    14. gst (Protective antigen)
    15. hla (Protective antigen)
    16. hlgB (Protective antigen)
    17. isdB (Protective antigen)
    18. IsdH (Protective antigen)
    19. lukF (Protective antigen)
    20. lukS (Protective antigen)
    21. MecA (Protective antigen)
    22. mntC (Protective antigen)
    23. sasA (Protective antigen)
    24. sdrD (Protective antigen)
    25. seb (Protective antigen)
    26. spa (Protective antigen)
    27. staphylococcal enterotoxin A (Protective antigen)
    28. traP (Protective antigen)
    29. TSST-1 (Protective antigen)
    30. aroA (Virmugen)
  3. Vaccine Related Host Genes
    1. Ifng (Interferon gamma)
    2. Il4 (interleukin 4)
  4. Vaccine Information
    1. S. aureus ClfA Protein Vaccine
    2. S. aureus CNA Protein Vaccine
    3. S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna
    4. S. aureus DNA Vaccine encoding PBP2a Protein
    5. S. aureus DNA vaccine pClfaSrtD13
    6. S. aureus FnbA Protein Vaccine
    7. S. aureus IsdB subunit vaccine (V710)
    8. Staphylococcus aureus aroA mutant vaccine
  5. References
I. General Information
1. NCBI Taxonomy ID:
1280
2. Disease:
Staph infections
3. Introduction
Staphylococcus aureus is a facultatively anaerobic, gram-positive coccus and is the most common cause of staph infections. It is frequently part of the skin flora found in the nose and on skin. S. aureus can cause a range of illnesses from minor skin infections, such as pimples, impetigo, boils (furuncles), cellulitis folliculitis, carbuncles, scalded skin syndrome, and abscesses, to life-threatening diseases such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome (TSS), chest pain, bacteremia, and sepsis. Its incidence is from skin, soft tissue, respiratory, bone, joint, endovascular to wound infections. It is still one of the five most common causes of nosocomial infections, often causing postsurgical wound infections (Wiki: S. aureus).
4. Microbial Pathogenesis
The initial critical event in most staphylococcal infections is the ability of bacteria to adhere to components within host tissues (Nilsson et al., 1998).
5. Host Ranges and Animal Models
S. aureus can survive on domesticated animals such as dogs, cats, and horses, and can cause bumblefoot in chickens. It is also a common commensal of human skin (Wiki: S. aureus). Mice are used as an animal model of infection (Senna et al., 2003).
6. Host Protective Immunity
In general, protection against S. aureus infection is attributed to intact epithelial and mucosal barriers and normal cellular and humoral responses (Nilsson et al., 1998).
1. aroA
  • Gene Name : aroA
  • Sequence Strain (Species/Organism) : Staphylococcus aureus subsp. aureus ED98
  • NCBI Gene ID : 8614197
  • NCBI Protein GI : 269203089
  • Locus Tag : SAAV_1451
  • Genbank Accession : CP001781
  • Protein Accession : YP_003282358
  • Taxonomy ID : 681288
  • Gene Starting Position : 1517365
  • Gene Ending Position : 1518663
  • Gene Strand (Orientation) : -
  • Protein Name : 3-phosphoshikimate 1-carboxyvinyltransferase
  • Protein pI : 4.84
  • Protein Weight : 44289.51
  • Protein Length : 432
  • DNA Sequence : Show Sequence
    >gi|269201690:1517365-1518663 Staphylococcus aureus subsp. aureus ED98, complete genome
    ATTATCCCTCATTTTCTAAAAGCTTTAGTTTTGGTAAAAATCCTGGAAATGATACATTTACAGCATCAAA
    TTGTTTGATTTTGACAGGCTCGCTTGAAAGTAGAGAAGCAACTGCAAGCATCATTCCTATTCGATGATCA
    GTTAAACTATCAACTGTTGCATTTGTTTTAAATTCTGACGGATGAATAATCAATCCATCATTAGTTGGTT
    GTAATTCAAACCCTAACAAGTTTAACATATCAGCCGTTGTATCAATTCTATTTGTTTCTTTTACTTTTAA
    TTCCTCGGCATCTTTAATTGTACTCGTGCCAACTGCTTGTGTACAAAGTAATGCTATTACAGGCAGTTCA
    TCAATTGCTTTTGGAACTAATTCTCCTTCGATTGTTATTGGTTGAAGCATTGGTGTGTATTGAATACGAA
    TAGAAGCAGTAGGTTCAGCACCAGTTGTTTGATTGAAAAGTTGGATATTACCGCCCATTTTTTCAACAAT
    ATCAATAATACCTGAACGTGTTGGATTGATTCCAACATTATGAATTGTTACATCACTTTCTGGTGTGATA
    AGTGCTGCAACAATAAAGAACGCTGCAGATGAAATATCGCCAGGAACATGAAAATCTGCAGGTTTAATAT
    GTTGAATGGCATCAGGGGTTGTAGTTATTGATAACCTTTCTGCTTCAATTGGAATATTAAAATGTCTGAA
    CATCGTCTCAGTATGATTTCGACTTACATCTAATTCCTTTATTACCGTTGTATCATTTGAAAACAAACTT
    GCAAATAAAATGGCACTTTTTACTTGTGCACTTGCAACTTCCATTTGATAATTTATACCTTTTATGACAG
    ATGGCTTAATAATTAATGGTGTATAATTATCTTCAATACCTTCAATATTCGCATCCATAAGTTTCAATGG
    TCTCAAGACACGATCCATTGGCCTTTTACCAATTGAAACATCGCCAGACAAAACACTTTCAATACCTAAA
    CCACTTAACAAACCAGCTAACAATCGAGTCGTTGTGCCAGAGTTTCCAGTATATAAAACTTGATGAGGTG
    TTTTAAAAGCTTTATATCCAGGTGAATTCACAACCAATTTATCTTCATCTTCTTTAATATCTACGCCTAA
    TAATCGGAATATATCCATTGTACGACGACAATCTTCACCAAGTAATGGTTTATAAATATTGGAAGTTCCT
    TCAGCTAAAGATGCTAACATGATTGCTCGATGTGTCATCGACTTATCTCCAGGCACTTCTATTTCACCTT
    TAAGTGGCCCTGAAATATCGATAATTTGTTCACTTACCA
  • Protein Sequence : Show Sequence
    >gi|269203089|ref|YP_003282358.1| 3-phosphoshikimate 1-carboxyvinyltransferase [Staphylococcus aureus subsp. aureus ED98]
    MVSEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGTSNIYKPLLGEDCRRTMDIFRLLGVDIKEDED
    KLVVNSPGYKAFKTPHQVLYTGNSGTTTRLLAGLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIE
    GIEDNYTPLIIKPSVIKGINYQMEVASAQVKSAILFASLFSNDTTVIKELDVSRNHTETMFRHFNIPIEA
    ERLSITTTPDAIQHIKPADFHVPGDISSAAFFIVAALITPESDVTIHNVGINPTRSGIIDIVEKMGGNIQ
    LFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTSTIKDAEELKVKETNRID
    TTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIGMMLAVASLLSSEPVKIKQFDAVNVSFPG
    FLPKLKLLENEG
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An aroA mutant is attenuated in mice and induced significant protection from challenge with wild type S. aureus (Buzzola et al., 2006).
  • Related Vaccine(s): Staphylococcus aureus aroA mutant vaccine
2. ClfA
  • Gene Name : ClfA
  • Sequence Strain (Species/Organism) : Staphylococcus aureus subsp. aureus str. Newman
  • VO ID : VO_0012390
  • NCBI Gene ID : 5330438
  • NCBI Protein GI : 151220968
  • Locus Tag : NWMN_0756
  • Genbank Accession : AP009351
  • Protein Accession : YP_001331790
  • 3D structure: PDB ID : 1N67
  • Taxonomy ID : 426430
  • Gene Starting Position : 849756
  • Gene Ending Position : 852557
  • Gene Strand (Orientation) : ?
  • Protein Name : clumping factor A
  • Protein pI : 3.44
  • Protein Weight : 91994.79
  • Protein Length : 933
  • Protein Note : fibrinogen-binding protein A
  • DNA Sequence : Show Sequence
    >NC_009641.1:849756-852557 Staphylococcus aureus subsp. aureus str. Newman DNA, complete genome
    AATGAATATGAAGAAAAAAGAAAAACACGCAATTCGGAAAAAATCGATTGGCGTGGCTTCAGTGCTTGTA
    GGTACGTTAATCGGTTTTGGACTACTCAGCAGTAAAGAAGCAGATGCAAGTGAAAATAGTGTTACGCAAT
    CTGATAGCGCAAGTAACGAAAGCAAAAGTAATGATTCAAGTAGCGTTAGTGCTGCACCTAAAACAGACGA
    CACAAACGTGAGTGATACTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCA
    GCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAG
    CTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGA
    ATTAGTGAATCAAACAAGTAATGAAACGACTTTTAATGATACTAATACAGTATCATCTGTAAATTCACCT
    CAAAATTCTACAAATGCGGAAAATGTTTCAACAACGCAAGATACTTCAACTGAAGCAACACCTTCAAACA
    ATGAATCAGCTCCACAGAGTACAGATGCAAGTAATAAAGATGTAGTTAATCAAGCGGTTAATACAAGTGC
    GCCTAGAATGAGAGCATTTAGTTTAGCGGCAGTAGCTGCAGATGCACCGGCAGCTGGCACAGATATTACG
    AATCAGTTGACGAATGTGACAGTTGGTATTGACTCTGGTACGACTGTGTATCCGCACCAAGCAGGTTATG
    TCAAACTGAATTATGGTTTTTCAGTGCCTAATTCTGCTGTTAAAGGTGACACATTCAAAATAACTGTACC
    TAAAGAATTAAACTTAAATGGTGTAACTTCAACTGCTAAAGTGCCACCAATTATGGCTGGAGATCAAGTA
    TTGGCAAATGGTGTAATCGATAGTGATGGTAATGTTATTTATACATTTACAGACTATGTAAATACTAAAG
    ATGATGTAAAAGCAACTTTGACCATGCCCGCTTATATTGACCCTGAAAATGTTAAAAAGACAGGTAATGT
    GACATTGGCTACTGGCATAGGTAGTACAACAGCAAACAAAACAGTATTAGTAGATTATGAAAAATATGGT
    AAGTTTTATAACTTATCTATTAAAGGTACAATTGACCAAATCGATAAAACAAATAATACGTATCGTCAGA
    CAATTTATGTCAATCCAAGTGGAGATAACGTTATTGCGCCGGTTTTAACAGGTAATTTAAAACCAAATAC
    GGATAGTAATGCATTAATAGATCAGCAAAATACAAGTATTAAAGTATATAAAGTAGATAATGCAGCTGAT
    TTATCTGAAAGTTACTTTGTGAATCCAGAAAACTTTGAGGATGTCACTAATAGTGTGAATATTACATTCC
    CAAATCCAAATCAATATAAAGTAGAGTTTAATACGCCTGATGATCAAATTACAACACCGTATATAGTAGT
    TGTTAATGGTCATATTGATCCGAATAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAAC
    TCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTG
    ACGGTATCGATAAACCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGA
    TTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGT
    GATTCTACATCAGATAGTGGTTCAGATTCAGCGAGTGATTCAGATTCAGCAAGTGATTCAGACTCAGCGA
    GTGATTCAGATTCAGCAAGCGATTCCGACTCAGCGAGCGATTCCGACTCAGACAATGACTCGGATTCAGA
    TAGCGATTCTGACTCAGACAGTGACTCAGATTCCGACAGTGACTCAGATTCAGATAGCGATTCTGACTCA
    GACAGTGACTCAGATTCAGATAGCGATTCAGATTCAGATAGCGATTCAGATTCCGACAGTGATTCCGACT
    CAGACAGCGATTCTGACTCCGACAGTGATTCCGACTCAGACAGCGATTCAGATTCCGACAGTGATTCCGA
    CTCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCA
    GATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATT
    CAGATTCCGACAGTGACTCAGATTCCGACAGTGACTCAGACTCAGACAGTGATTCGGATTCAGCGAGTGA
    TTCGGATTCAGATAGTGATTCCGACTCCGACAGTGACTCGGATTCAGATAGCGACTCAGACTCGGATAGC
    GACTCGGATTCAGATAGCGATTCGGACTCAGATAGCGATTCAGAATCAGACAGCGATTCAGAATCAGACA
    GCGATTCAGATTCAGACAGCGACTCAGACAGTGACTCAGATTCAGATAGTGACTCGGATTCAGCGAGTGA
    TTCAGACTCAGGTAGTGACTCCGATTCATCAAGTGATTCCGACTCAGAAAGTGATTCAAATAGCGATTCC
    GAGTCAGGTTCTAACAATAATGTAGTTCCGCCTAATTCACCTAAAAATGGTACTAATGCTTCTAATAAAA
    ATGAGGCTAAAGATAGTAAAGAACCATTACCAGATACAGGTTCTGAAGATGAAGCAAATACGTCACTAAT
    TTGGGGATTATTAGCATCAATAGGTTCATTACTACTTTTCAGAAGAAAAAAAGAAAATAAAGATAAGAAA
    TA
    
    
  • Protein Sequence : Show Sequence
    >YP_001331790.1 clumping factor A [Staphylococcus aureus subsp. aureus str. Newman]
    MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSVTQSDSASNESKSNDSSSVSAAPKTDD
    TNVSDTKTSSNTNNGETSVAQNPAQQETTQSSSTNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEE
    LVNQTSNETTFNDTNTVSSVNSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSA
    PRMRAFSLAAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFKITVP
    KELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLTMPAYIDPENVKKTGNV
    TLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNNTYRQTIYVNPSGDNVIAPVLTGNLKPNT
    DSNALIDQQNTSIKVYKVDNAADLSESYFVNPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVV
    VNGHIDPNSKGDLALRSTLYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPED
    SDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSDNDSDSD
    SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
    SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASD
    SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASD
    SDSGSDSDSSSDSDSESDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLI
    WGLLASIGSLLLFRRKKENKDKK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice immunized with recombinant ClfA and challenged with S. aureus developed less-severe arthritis than did mice immunized with a control antigen (Josefsson et al., 2001).
  • Related Vaccine(s): S. aureus ClfA Protein Vaccine , S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna , S. aureus DNA vaccine pClfaSrtD13
3. clfA
  • Gene Name : clfA
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : AEK27206
  • Other Database IDs : CDD:287406
  • Taxonomy ID : 1280
  • Protein Name : ClfA
  • Protein pI : 4.19
  • Protein Weight : 38465.04
  • Protein Length : 443
  • Protein Note : C-terminus of bacterial fibrinogen-binding adhesin; pfam10425
  • Protein Sequence : Show Sequence
    >AEK27206.1 ClfA, partial [Staphylococcus aureus]
    EESVNQTSNETTSNDTNTVSSVNSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNT
    SAPRMRAFSLAAVAADAPAAGTDITNQLTNVTVGIDSGDTVYPHQAGYVKLNYGFSVPNSAVKGDTFKIT
    VPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYINTKDDVKATLTMPAYIDPENVTKTG
    NVTLATGIGNTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNNTYRQTIYVNPSGDNVIAPVLTGNLKP
    NTDSNALIDQQNTSIKVYKVDNAADLSESYFVNPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYI
    VVVNGHIDPNSKGDLALRSTLYGYDSRFVWRSMSWD
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Lacey et al., 2017)
4. clfB
  • Gene Name : clfB
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : SIN33476
  • Other Database IDs : CDD:309682
    CDD:313621
    CDD:307064
  • Taxonomy ID : 46170
  • Gene Strand (Orientation) : ?
  • Protein Name : Fibrinogen-binding protein B
  • Protein pI : 3.66
  • Protein Weight : 93865.65
  • Protein Length : 1009
  • Protein Note : predicted cleavage at residue 45
  • Protein Sequence : Show Sequence
    >SIN33476.1 Fibrinogen-binding protein B [Staphylococcus aureus subsp. aureus]
    MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQASEQSNDTTQSSKNNASADSEKNNMIE
    TPQLNTTANDTSDISANTNSANVDSTTKPMSTQTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQ
    EANSQVDNKTTNDANSIATNSELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADA
    KGTNVNDKVTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNSNNTM
    PIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKAPKSGTYDANINIADEM
    FNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQTVFVNPKQRVLGNTWVYIKGYQDKIEES
    SGKVSATDTKLRIFEVNDTSKLSDSYYADPNDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVE
    GHYDNTGKNLKTQVIQENVDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDP
    EPEPEPTPDPEPSPDPEPEPSPDPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDSDSDSESDSDS
    ESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSESDSDSDSDSDSDSDSDSDSDSDS
    DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
    DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
    DSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHKTDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQD
    HKEKA
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Rauch et al., 2014)
5. cna
  • Gene Name : cna
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • VO ID : VO_0012389
  • NCBI Nucleotide GI : 387879
  • NCBI Protein GI : WP_001039420
  • Protein Accession : AAA20874.1
  • 3D structure: PDB ID : 2F68
  • Other Database IDs : CDD:283410
    CDD:283411
    CDD:301706
    CDD:212461
  • Taxonomy ID : 1280
  • Gene Strand (Orientation) : ?
  • Protein Name : Cna B-type domain-containing protein
  • Protein pI : 6.09
  • Protein Weight : 124972.57
  • Protein Length : 1278
  • Protein Note : Collagen binding domain; pfam05737
  • DNA Sequence : Show Sequence
    >gi|387879|gb|M81736.1|STACNA Staphylococcus aureus collagen adhesin (cna) gene, complete cds
    ATGCACTTGTATTCGTTATACTGTATATATTTTGCATAATAAAATAATAATATGAATTTTTGATAAATTT
    CATTGAATAAGAACTAAATTAGTTTATAATTTATTATTAGTATCCTGTGGATATGACATAGAGTATAAGG
    AGGGGTTTTTATGAACAAAAATGTGTTGAAGTTTATGGTCTTTATAATGTTATTAAATATCATCACACCT
    TTATTTAATAAAAATGAAGCATTTGCAGCACGAGATATTTCATCAACGAATGTTACAGATTTAACTGTAT
    CACCGTCTAAGATAGAAGATGGTGGTAAAACGACAGTAAAAATGACGTTCGACGATAAAAATGGAAAAAT
    ACAAAATGGTGACATGATTAAAGTGGCATGGCCGACAAGCGGTACAGTAAAGATAGAGGGTTATAGTAAA
    ACAGTACCATTAACTGTTAAAGGTGAACAGGTGGGTCAAGCAGTTATTACACCAGACGGTGCAACAATTA
    CATTCAATGATAAAGTAGAAAAATTAAGTGATGTTTCGGGATTTGCAGAATTTGAAGTACAAGGAAGAAA
    TTTAACGCAAACAAATACTTCAGATGACAAAGTAGCTACGATAACATCTGGGAATAAATCAACGAATGTT
    ACGGTTCATAAAAGTGAAGCGGGAACAAGTAGTGTTTTCTATTATAAAACGGGAGATATGCTACCAGAAG
    ATACGACACATGTACGATGGTTTTTAAATATTAACAATGAAAAAAGTTATGTATCGAAAGATATTACTAT
    AAAGGATCAGATTCAAGGTGGACAGCAGTTAGATTTAAGCACATTAAACATTAATGTGACAGGTACACAT
    AGCAATTATTATAGTGGACAAAGTGCAATTACTGATTTTGAAAAAGCCTTTCCAGGTTCTAAAATAACTG
    TTGATAATACGAAGAACACAATTGATGTAACAATTCCACAAGGCTATGGGTCATATAATAGTTTTTCAAT
    TAACTACAAAACCAAAATTACGAATGAACAGCAAAAAGAGTTTGTTAATAATTCACAAGCTTGGTATCAA
    GAGCATGGTAAGGAAGAAGTGAACGGGAAATCATTTAATCATACTGTGCACAATATTAATGCTAATGCCG
    GTATTGAAGGTACTGTAAAAGGTGAATTAAAAGTTTTAAAACAGGATAAAGATACCAAGGCTCCTATAGC
    TAATGTAAAATTTAAACTTTCTAAAAAAGATGGATCAGTTGTAAAGGACAATCAAAAAGAAATTGAGATT
    ATAACAGATGCAAACGGTATTGCTAATATTAAAGCGTTGCCTAGTGGAGACTATATTTTAAAAGAAATAG
    AGGCGCCACGACCGTATACATTTGATAAGGATAAAGAATATCCGTTTACTATGAAAGATACAGATAATCA
    GGGATATTTTACGACTATTGAAAATGCAAAAGCGATAGAAAAAACAAAAGATGTTTCTGCTCAAAAGGTT
    TGGGAAGGCACTCAAAAAGTGAAACCAACGATTTATTTCAAGTTGTACAAACAAGATGACAATCAAAATA
    CAACACCAGTAGACAAAGCAGAGATTAAAAAATTAGAAGATGGAACGACAAAAGTGACATGGTCTAATCT
    TCCGGAAAATGACAAAAATGGCAAGGCTATTAAATATTTAGTTAAAGAAGTAAATGCTCAAGGTGAAGAT
    ACAACACCAGAAGGATATACTAAAAAAGAAAATGGTTTAGTGGTTACTAATACTGAAAAACCAATCGAAA
    CAACATCAATTAGTGGTGAAAAAGTATGGGACGACAAAGACAATCAAGATGGTAAGAGACCAGAAAAAGT
    CAGTGTGAATTTATTGGCTAACGGGGAGAAAGTAAAAACGTTAGACGTGACATCTGAAACAAACTGGAAG
    TACGAATTTAAAGACTTACCGAAGTATGATGAAGGAAAGAAAATAGAATATACAGTGACCGAAGATCACG
    TAAAAGACTACACAACAGACATCAACGGTACGACAATAACGAACAAGTATACACCAGGAGAGACATCGGC
    AACAGTAACAAAAAATTGGGATGACAATAATAACCAAGACGGAAAACGACCAACTGAAATCAAAGTTGAG
    TTATATCAAGATGGAAAAGCAACAGGAAAAACGGCAATATTAAATGAATCTAATAACTGGACACATACGT
    GGACAGGATTAGATGAAAAAGCAAAAGGACAACAAGTAAAATACACAGTCGAGGAATTAACAAAGGTCAA
    AGGTTATACAACACATGTGGATAACAATGATATGGGTAACTTGATTGTGACGAATAAATATACGCCAGAA
    ACAACATCAATTAGTGGTGAAAAAGTATGGGACGACAAAGACAATCAAGATGGTAAGAGACCAGAAAAAG
    TCAGTGTGAATTTATTGGCTGATGGAGAGAAAGTAAAAACGTTAGACGTGACATCTGAAACAAACTGGAA
    GTACGAATTTAAAGACTTACCGAAGTATGATGAAGGAAAGAAAATAGAATATACAGTGACCGAAGATCAC
    GTAAAAGACTACACAACAGACATCAACGGTACGACAATAACGAACAAGTATACACCAGGAGAGACATCGG
    CAACAGTAACAAAAAATTGGGATGACAATAATAACCAAGACGGAAAACGACCAACTGAAATCAAAGTTGA
    GTTATATCAAGATGGAAAAGCAACAGGAAAAACGGCAATATTAAATGAATCTAATAACTGGACACATACG
    TGGACAGGATTAGATGAAAAAGCAAAAGGACAACAAGTAAAATACACAGTCGAGGAATTAACAAAGGTCA
    AAGGTTATACAACACATGTGGATAACAATGATATGGGCAACTTGATTGTGACGAATAAATATACGCCAGA
    AACAACATCAATTAGTGGTGAAAAAGTATGGGACGACAAAGACAATCAAGATGGTAAGAGACCAGAAAAA
    GTCAGTGTGAATTTATTGGCTAACGGAGAGAAAGTAAAAACGTTAGACGTGACATCTGAAACAAACTGGA
    AGTACGAATTTAAAGACTTACCGAAGTATGATGAAGGAAAGAAAATAGAATATACAGTGACCGAAGATCA
    CGTAAAAGACTACACAACAGACATCAACGGTACGACAATAACGAACAAGTATACACCAGGAGAGACATCG
    GCAACAGTAACAAAAAATTGGGATGACAATAATAACCAAGACGGAAAACGACCAACTGAAATCAAAGTTG
    AGTTATATCAAGATGGAAAAGCAACAGGAAAAACGGCAATATTAAATGAATCTAATAACTGGACACATAC
    GTGGACAGGATTAGATGAAAAAGCAAAAGGACAACAAGTAAAATACACAGTCGATGAATTAACAAAAGTT
    AATGGCTATACAACGCATGTGGATAACAATGATATGGGTAACTTGATTGTGACAAATAAATATACGCCGA
    AAAAACCGAATAAACCAATCTATCCTGAAAAACCAAAAGACAAAACACCACCAACTAAACCTGATCATTC
    TAATAAAGTTAAACCAACTCCCCCAGATAAGCCATCAAAAGTGGATAAGGATGATCAACCTAAAGATAAT
    AAAACCAAACCTGAAAATCCTCTAAAAGAATTACCAAAAACTGGTATGAAGATTATAACTTCATGGATTA
    CATGGGTATTTATAGGTATATTGGGACTGTATTTAATTTTAAGAAAAAGATTTAACTCATAAACCATTAT
    AATTATTTTTATAGATAAGGCTATTCTTAGTTCTATGTATAATACATGTATATTAATAGGTCACTTTTAA
    TCTGTATGTAAGCAGACTAAGAGTGGCCTTTTAAACAAATAAAAAAA
  • Protein Sequence : Show Sequence
    >WP_001039420.1 Cna B-type domain-containing protein [Staphylococcus aureus]
    MNKNVLKFMVFIMLLNIITPLFNKNEALAARDISSTNVTDLTVSPTKIEDGGKTTVKMTFDDKSVKIQNG
    DTIKVAWPTSGTVKIEGYSKTVPLTVKGEQVGQAVITPDGATITFNDKVEKLSDVSGFAEFEVQGRNLTQ
    TNTSDDKVATITSGNKSTNVTVHKSEAGTSSVFYYKTGDMLPEDTTHVRWFLNINNEKRYVSKDITIKDQ
    IQGGQQLDLSTLNINVTGTHSNYYSGSNAITDFEKAFPGSKITVDNTKNTIDVTIPQGYGSYNSFSINYK
    TKITNEQQKEFVNNSQAWYQEHGKEEVNGKSFNHTVHNINANAGIEGTVKGELKVLKQDKDTKAPIANVK
    FKLSKKDGSVVKDNQKEIEIITDANGTANIKALPSGDYILKEIEAPAPYTFDKDKEYPFTMKDTDNQGYF
    TTIENAKEIEKTKDVSAQKVWEGTQKVKPTIYFKLYKQDDNQNTTPVDKAEIKKLEDGTTKVTWSNLPEN
    DKNGKTIKYLVKEVNAQGEDTTPEGYTKKENGLVVTNTEKPIETTSISGEKVWDDKDNQDGKRPEKVSVN
    LLANGEKVKTLDVTSETNWKYEFKNLPKYDEGKKIEYTVTEDHVKDYTTDINGTTITNKYTPGETSATVT
    KNWDDNNNQDGKRPTEIKVELYQDGKATGKTATLNESNNWTHTWAGLDEKAKGQQVKYTVEELTKVKGYT
    THVDNNDMGNLIVTNKYTPETTSISGEKVWDDKDNQDGKRPEKVSVNLLANGEKVKAVDVTSETNWKYEF
    KDLPKYDEGKKIEYTVTEDHVKDYTTDINGTTITNKYTPGETSATVTKNWDDNNNQDGKRPTEIKVELYQ
    DGKATGKTAKLNESNNWTHTWTGLDEKAKGQQVKYTVEELTKVKGYTTHVDNNDMGNLIVTNKYTPETTS
    INGEKVWDDKDNQDGKRPEKVSINLLANGEKVKTLDVTSETNWKYEFKDLPKYDEGKKIEYTVTEDHVKD
    YTTDINGTTITNKYTPGETSATVTKNWDDNNNQDGKRPTEIKVELYQDGKATGKTATLNESNNWTHTWAG
    LDEKAKGQQVKYTVEELTKVKGYTTHVDNNDMGNLIVTNKYTPEKPNKPIYPEKPKDKTPPTKPDHSNKV
    KPTPPDKPSKVDKDDQPKDNKTKPENPLKELPKTGMKIITSWITWVFIGILGLYLILRKRFNS
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice were immunized with a recombinant fragment of CNA protein, and were then challenged with the S. aureus clinical isolate strain Phillips. At 14 d after inoculation, mortality in the collagen adhesin-vaccinated group was only 13%, compared with 87% in the control antigen immunized group (Nilsson et al., 1998).
    Mice vaccinated with a combination of two Staphylococcus aureus antigens consisting of a recombinant collagen-binding protein (CnBP) and alpha-toxoid (alpha-toxoid) were significantly protected from intramammary challenge infection with S. aureus.(Mamo et al., 2000)
  • Related Vaccine(s): S. aureus CNA Protein Vaccine , S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna
6. Csa1A
  • Gene Name : Csa1A
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : 4BIH_B
  • Other Database IDs : CDD:282376
  • Taxonomy ID : 93061
  • Protein Name : Csa1A
  • Protein pI : 6.96
  • Protein Weight : 28371.75
  • Protein Length : 318
  • Protein Note : Protein of unknown function, DUF576; pfam04507
  • Protein Sequence : Show Sequence
    >pdb|4BIH|B Chain B, Uncharacterized Lipoprotein Saouhsc_00053
    MGSSHHHHHHENLYFQGGCGIGKEAEVKKSFEKTLSMYPIKNLEDLYDKEGYRDDQFDKNDKGTWIINSE
    MVIQPNNEDMVAKGMVLYMNRNTKTTNGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDEKI
    KKEIENFKFFVQYGDFKNLKNYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTSKAPKLLLKG
    SGNLKGSSVGYKDIEFTFVEKKEENIYFSDSLDYKKSGDV
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Bagnoli et al., 2015)
7. efb
  • Gene Name : efb
  • Sequence Strain (Species/Organism) : Staphylococcus aureus subsp. aureus VRS11b
  • NCBI Protein GI : 387748005
  • Other Database IDs : CDD:192953
  • Taxonomy ID : 1116225
  • Gene Strand (Orientation) : ?
  • Protein Name : extracellular fibrinogen-binding protein Efb
  • Protein pI : 10.45
  • Protein Weight : 18630.02
  • Protein Length : 271
  • Protein Note : Extracellular fibrinogen binding protein C terminal; pfam12199
  • Protein Sequence : Show Sequence
    >EIK34704.1 extracellular fibrinogen-binding protein Efb [Staphylococcus aureus subsp. aureus VRS11b]
    MKNKLIAKSLLTIAAIGITTTTIASTADASEGYGPREKKPVSINHNIVEYNDGTFKYQSRPKFNSTPKYI
    KFKHDYNILEFNDGTFEYGARPQFNKPAAKTDATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLV
    SFEYKVKKMVLQERIDNVLKQGLVR
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna
8. entA_1
  • Gene Name : entA_1
  • Sequence Strain (Species/Organism) : Staphylococcus aureus subsp. aureus SK1585
  • NCBI Protein GI : KFD31781
  • Taxonomy ID : 1274985
  • Protein Name : sea
  • Protein pI : 6.05
  • Protein Weight : 28560.84
  • Protein Length : 316
  • Protein Note : SEA
  • Protein Sequence : Show Sequence
    >KFD31781.1 sea [Staphylococcus aureus subsp. aureus SK1585]
    MGELEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKLDSKRLYNAKVSFANPTDLENKKTNDRLLKH
    DLLFHDMFVNDDWKKDFKVEFENEALSKKFINKDIDIFAGNYGYGCHGGETNKTQCSYGGVTLSDNNKYD
    NDKNIPCNLWIDGHQTEIELTAVKTKKKIVTIQELDVQLRNYLNEKYKLYEQGGDIVKGYVKYHNDDEKN
    VEYDFYNLNGEYGHEVLKMYADNKTINSDKLHLDIYLFKS
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Nilsson et al., 1999)
9. esxA
  • Gene Name : esxA
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : A0A0H2XI99
  • Other Database IDs : CDD:227179
  • Taxonomy ID : 367830
  • Protein Name : ESAT-6 secretion system extracellular protein A
  • Protein pI : 4.34
  • Protein Weight : 13241.094
  • Protein Length : 225
  • Protein Note : Ess extracellular protein A
  • Protein Sequence : Show Sequence
    >sp|A0A0H2XI99.1|ESXA_STAA3 RecName: Full=ESAT-6 secretion system extracellular protein A; Short=Ess extracellular protein A
    MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWEGQAFSRFEEQFQQLSPKVEKFAQLLE
    EIKQQLNSTADAVQEQDQQLSNNFGLQ
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Bagnoli et al., 2015)
10. esxB
  • Gene Name : esxB
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : A0A0H2XIE9
  • Other Database IDs : CDD:310537
  • Taxonomy ID : 367830
  • Protein Name : ESAT-6 secretion system extracellular protein B
  • Protein pI : 4.94
  • Protein Weight : 13333.384
  • Protein Length : 232
  • Protein Note : Ess extracellular protein B
  • Protein Sequence : Show Sequence
    >sp|A0A0H2XIE9.1|ESXB_STAA3 RecName: Full=ESAT-6 secretion system extracellular protein B; Short=Ess extracellular protein B
    MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEGSDWEGQFANKVKDVLLIMAKFQEELV
    QPMADHQKAIDNLSQNLAKYDTLSIKQGLDRVNP
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Bagnoli et al., 2015)
11. Fhud2
  • Gene Name : Fhud2
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : 4FNA_C
  • Other Database IDs : CDD:223687
    CDD:294188
    CDD:238347
  • Taxonomy ID : 1280
  • Protein Name : Fhud2
  • Protein pI : 9.5
  • Protein Weight : 30363.45
  • Protein Length : 336
  • Protein Note : NCBI Domains
  • Protein Sequence : Show Sequence
    >pdb|4FNA|C Chain C, Ferric Hydroxamate Receptor 2
    GSNNKAETKSYKMDDGKTVDIPKDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIG
    DGDVEKVAKEKPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWEET
    TAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQKLTAKAGWAEVKQEE
    IEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAGTYWYNDPYTLDFMRKDLKEKLIKAAK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Bagnoli et al., 2015)
12. fnbA
  • Gene Name : fnbA
  • Sequence Strain (Species/Organism) : Staphylococcus aureus subsp. aureus NCTC 8325
  • VO ID : VO_0012392
  • NCBI Protein GI : 120457
  • Other Database IDs : CDD:309682
    CDD:331378
    CDD:313621
    CDD:308566
    CDD:307064
  • Taxonomy ID : 93061
  • Gene Strand (Orientation) : ?
  • Protein Name : Fibronectin-binding protein A
  • Protein pI : 4.36
  • Protein Weight : 105986.3
  • Protein Length : 1133
  • Protein Note : FnbpA
  • Protein Sequence : Show Sequence
    >sp|P14738.1|FNBA_STAA8 RecName: Full=Fibronectin-binding protein A; Short=FnbpA; Flags: Precursor
    MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTTVEENGNSATDNKTSETQTTATNVNHI
    EETQSYNATVTEQPSNATQVTTEEAPKAVQAPQTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQR
    QVDLTPKKATQNQVAETQVEVAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEG
    HNNTNKVEPHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGEVLEG
    GKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELDVKYKDGIGNYYANLNG
    SIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNGNQPKVRIFEYLGNNEDIAKSVYANTTDT
    SKFKEVTSNMSGNLNLQNNGSYSLNIENLDKTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGY
    TLTWDNGLVLYSNKANGNEKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAI
    DGGGGYVDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHADVVEYE
    EDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIELVDELPEEHGQAQGPVEE
    ITKNNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNI
    VDIDFDSVPQIHGQNKGNQSFEEDTEKDKPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSY
    QFGGHNSVDFEEDTLPKVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPP
    TPEVPSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKPQSKKS
    ELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice were immunized with fusion proteins encompassing the fibronectin-binding domain of a staphylococcal fibronectin-binding protein (FnBP-A). Vaccination of mice with recombinant FnBP resulted in significant protection against challenge with S. aureus (Mamo et al., 1994).
  • Related Vaccine(s): S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna , S. aureus FnbA Protein Vaccine
13. FnbpA
  • Gene Name : FnbpA
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : EEV76353
  • Other Database IDs : CDD:287406
  • Taxonomy ID : 553573
  • Gene Strand (Orientation) : ?
  • Protein Name : fibrinogen-binding protein A
  • Protein pI : 3.38
  • Protein Weight : 97931.47
  • Protein Length : 1076
  • Protein Note : C-terminus of bacterial fibrinogen-binding adhesin; pfam10425
  • Protein Sequence : Show Sequence
    >EEV76353.1 fibrinogen-binding protein A [Staphylococcus aureus A8115]
    MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSVTQSDSASNESKSNDSSSVSAAPKTDD
    TNVSDTKTSSNTNNGETSVAQNPAQQETTQSSSTNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEE
    LVNQTSNETTSNDTNTVSSVNSPQNSTNAENVSTTQDTSTEATPSNNESAPQNTDASNKDVVSQAVNPST
    PRMRAFSLAAVAADAPAAGTDITNQLTDVKVTIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFKITVP
    KELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVDNKENVTANITMPAYIDPENVTKTGNV
    TLTTGIGTNTASKTVLIDYEKYGQFHNLSIKGTIDQIDKTNNTYRQTIYVNPSGDNVVLPALTGNLIPNT
    KSNALIDAKNTDIKVYRVDNANDLSESYYVNPSDFEDVTNQVRISFPNANQYKVEFPTDDDQITTPYIVV
    VNGHIDPASTGDLALRSTFYGYDSNFIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPED
    SDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSA
    SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
    SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
    SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSD
    SDSDSDSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDND
    SDSDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLASLGSLLLFR
    RKKENKDKK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Veloso et al., 2015)
14. gapC
  • Gene Name : gapC
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : AAR13672
  • Other Database IDs : CDD:278473
    CDD:273675
    CDD:280894
  • Taxonomy ID : 1280
  • Protein Name : GapC
  • Protein pI : 4.71
  • Protein Weight : 33224.4
  • Protein Length : 383
  • Protein Note : Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain; pfam00044
  • Protein Sequence : Show Sequence
    >AAR13672.1 GapC [Staphylococcus aureus]
    MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHLLKYDTMQGRFTGEVEVVDGGFRVNGK
    EVKSFSEPDASKLPWKDLNIDVVLECTGFYTDKDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELD
    GSETVVSGASCTTNSLAPVAKVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNS
    TGAAKAIGKVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKPDVTVEQVNEAMKNASNESFGYTEDEIVS
    SDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAELSK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Yu et al., 2014)
15. gst
  • Gene Name : gst
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : EES94142
  • Taxonomy ID : 450394
  • Protein Name : glutathione S-transferase
  • Protein pI : 6.24
  • Protein Weight : 9384
  • Protein Length : 182
  • Protein Note : COG: COG0625; Pfam: PF02798; InterPro: IPR012335
  • Protein Sequence : Show Sequence
    >EES94142.1 glutathione S-transferase, partial [Staphylococcus aureus subsp. aureus USA300_TCH959]
    TLNHRQKELPMKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYFAVNPKGQVPALLLDDG
    TLLTEGVAIM
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Cui et al., 2005)
16. hla
  • Gene Name : hla
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : AMQ79365
  • Other Database IDs : CDD:273388
  • Taxonomy ID : 1280
  • Protein Name : hla
  • Protein pI : 9.19
  • Protein Weight : 34393.79
  • Protein Length : 365
  • Protein Note : beta-channel forming cytolysin; TIGR01002
  • Protein Sequence : Show Sequence
    >AMQ79365.1 hla [Staphylococcus aureus]
    MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNTTVKTGDLVTYDKENGMHKKVFYSFID
    DKNHNKKILVIRTKGTIAGQYRVYSEEGANKSGLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMS
    TLTYGFNGNVTGDDSGKIGGLIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDR
    DSWNPVYGNQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKATKQQTNIDVIYERVRD
    DYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Bagnoli et al., 2015)
17. hlgB
  • Gene Name : hlgB
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : AKB00489
  • Other Database IDs : CDD:273388
  • Taxonomy ID : 1280
  • Protein Name : gamma-hemolysin component C precursor, HlgB
  • Protein pI : 9.81
  • Protein Weight : 35450.76
  • Protein Length : 411
  • Protein Note : beta-channel forming cytolysin; TIGR01002
  • Protein Sequence : Show Sequence
    >AKB00489.1 gamma-hemolysin component C precursor, HlgB [Staphylococcus aureus]
    MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTLYKTTATADSDKFKISQILTFNFIKDK
    SYDKDTLVLKATGNINSGFVKPNPNDYDFSKLYWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTL
    GYTFGGDISISNGLSGGLNGNTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSF
    HPTYGNELFLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMDLYQI
    RWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Delfani et al., 2016)
18. isdB
  • Gene Name : isdB
  • Sequence Strain (Species/Organism) : Staphylococcus aureus subsp. aureus str. Newman
  • NCBI Gene ID : 5330644
  • NCBI Protein GI : 151221252
  • Locus Tag : NWMN_1040
  • Genbank Accession : AP009351
  • Protein Accession : YP_001332074
  • Other Database IDs : CDD:213844
  • Taxonomy ID : 426430
  • Gene Starting Position : 1142079
  • Gene Ending Position : 1144016
  • Gene Strand (Orientation) : -
  • Protein Name : iron-regulated heme-iron binding protein IsdB
  • Protein pI : 9.6
  • Protein Weight : 67826.06
  • Protein Length : 645
  • Protein Note : heme uptake protein IsdB; TIGR03657
  • DNA Sequence : Show Sequence
    >NC_009641.1:1142079-1144016 Staphylococcus aureus subsp. aureus str. Newman DNA, complete genome
    ATTAGTTTTTACGTTTTCTAGGTAATACGAATGCAACGATGCTACTTAAAGCTAATAATGCCATTAATGG
    TAATGTCATATCTTTATTTGATTCTTCACCAGTTTGTGGTAATGATTTTGCTTTATTTTCTTGTGTATTT
    TTATTGTTTTGGCTTTGAGTGTGTCCATCATTTGTGTTTTTAATGTTTGCTTTTTGTAATGGAGCACTAT
    CTTTTGCTTCGCTAGAACCTGCTGAAGTTTGAACAACATCTTTTGTTGTTTTTGATGAAGCAGTTGTTGG
    TTTTGCAACATTTTGAGTCGTAGATACTACCTTAGTTGGAGTTGTACTACTTGATTCTACTTCACCTTTA
    GTTGGTTTTGTAGCAGGCGTTTTGTCTTTACCTGACTCACTAGATGCGTCATTTTCTTTTTCAACACTTG
    GTAATTGTTTATTGTCATCTTTTTGGCTGTCTTGTTTTTGTGATTCTTTTTCAACAGGTGATGGTGTTGG
    TTTGCTAGGCGTAGCTGGAGTAGCTTCCTTCTTAGCTGAGTTATCTTGTTGTTCTTTTTTGTTAGATTTA
    TCGGTATTGGCTTTTGTAAATGCTTCTTTATCAACGATTCTGACATGGTATTGTCCATCATAATCAATCG
    TTTTTACGTGAACTTTAACGATAGCATCATATAGAGTTTTACCTTCAACATATGGGAAAATAATTGTTCT
    AGTATTATTTTTAGCATCTTTGCTTATAGTTCTAACACGTTGACCTTCAACCATGAAATCTTTCCAGTAA
    TCGTCATTAGTAGTTTCCATGACCATATATTTTTTGCCGTTAAGCATACCTGTTTTAATAGGGTGTTTAA
    CAAAAGTATCCATCATAGATTCGTTATTCTCAACACTTTCATAAACAACATATTTTGTATCTTGTAAATC
    AGTCATTTTTTCATTTGTTGGTTGTACATTTTGGAATTCAGTAATAGCTGATTTCACTTGCTCATCTAAA
    GCTTTCTTTGTATCCTCTAATTTCTTCTTGTACTCAGCCTTTAATTTTTCAGGAAGTTTATCTTGAATTT
    TATTTAATTCATAAACTTGTCTTTCTAGTGTTTTCGCTTTTTTATATGGCGCTAATAATTTTTCAGCTTT
    ATAATCTTCTTCAGTTTTGAATTTATCTGCACTGTTATAAATTGGTTGTGCGAATTCCATTAATGTGTAA
    TCGTATTTTTCTTCTTTGTTATTGAAGTGTGTTGAACTAACAATTTTAACAGCTTTTGTTCCGTTTGATA
    CAGAGAAGCGAATGTAAGCATAATCTTTAACAGTATCGTATGATACTAATTTAATTGGCAACTTTTTGTC
    ACCTTCATAAACTTCAAATTTTCTCCAAAATTGACCTGATTGTAATCCTAATTCAATTTCTGGTTTTGAA
    TCAGTGAAAATAACTCTAGCAGGTTTAACAGAACTTGCATAATGATAAAACTGTTGAGTTCCATCTTTCT
    TTTTCATTTCAAAATCAATTGGACGAGAGTTTGGTGCGCTATGATCTTTGTCTTTTATTGCAGGGTTTTT
    AATCGCTTCTCTAAGTTCCTGATTCAAAATAGGATATGTATTGTTAGTGGCTTTTGCTGCTGGTTTAACT
    TCTTTTGTTTCCTTAGGGGCTTTAACTTCTTTAACTTCTTTAGCTTCTTTTGTTTCAGAAGTAGGGGCCT
    CAACTTCTTTATTAGATACTGAGACAGCATTAGCTACTGGTTTAGTTTCTGGAGCTTTTTCAGATGTTGT
    TGTTGGACTTGCAACTGCTTCAGTTTTTGGTTGTGCTTCTGTATTTGTACCACCTGTTTCTTCAGCTGCT
    GCTTGTGCTTCGCCATTTGACATTAATAATAAAAGTGTACTAATTGCTACAGATGCAACGCCTAGTGATG
    ACTTTCTAATTGAATAAAATGATTTAAATTCTTTTTGCTGTTTGTTCA
    
    
  • Protein Sequence : Show Sequence
    >YP_001332074.1 iron-regulated heme-iron binding protein IsdB [Staphylococcus aureus subsp. aureus str. Newman]
    MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEETGGTNTEAQPKTEAVASPTTTSEKAP
    ETKPVANAVSVSNKEVEAPTSETKEAKEVKEVKAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKD
    KDHSAPNSRPIDFEMKKKDGTQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIK
    LVSYDTVKDYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAEKLLA
    PYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQNVQPTNEKMTDLQDTKY
    VVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWKDFMVEGQRVRTISKDAKNNTRTIIFPY
    VEGKTLYDAIVKVHVKTIDYDGQYHVRIVDKEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVE
    KESQKQDSQKDDNKQLPSVEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSK
    TTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLPLMALLA
    LSSIVAFVLPRKRKN
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : In preclinical studies, a isdB vaccine has shown protection from lethal infection in mice (Harro et al., 2010).
    Iron-regulated surface determinant B (IsdB) of Staphylococcus aureus (S. aureus) is a highly conserved surface protein that can induce protective CD4(+) T-cell immune response. A pivotal role of CD4(+) T-cells in effective immunity against S. aureus infection has been proved(Yu et al., 2015)
19. IsdH
  • Gene Name : IsdH
  • Sequence Strain (Species/Organism) : Staphylococcus aureus subsp. aureus 71193
  • NCBI Protein GI : AFH69961
  • Other Database IDs : CDD:331945
  • Taxonomy ID : 1155084
  • Protein Name : IsdH
  • Protein pI : 5.06
  • Protein Weight : 94952.35
  • Protein Length : 970
  • Protein Note : methicillin-sensitive; sequence type ST398
  • Protein Sequence : Show Sequence
    >AFH69961.1 IsdH [Staphylococcus aureus subsp. aureus 71193]
    MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENTNTSDKISENQNNNATTTQPPKDTNQT
    QPATQPTNTAKTYPAADESLKDAIKNPAVENKEHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYT
    KKKAEVELDINTASTWKKFEVYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGAET
    NYDYTKLVFAKPIYNDPSLVKSDTNDAVASNDQSSSDASNQTNTNTSNQNTSTTNNASDQPQATTNMSQP
    AKPVSSANADQASSQPAHETNSNGNTNDKTNASSNQSNGNQQYPPADESLQDAIKNPAIIDKEHTADNWR
    PIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKTASTWKKFEVYEGDKKLPVELVSYDSDKD
    YAYIRFPVSNGAREVKIVSSIEYGENFHEDYDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTL
    ERQVYELEKLQDKLPEKYKAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEEN
    SESVMDGFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYVSDKAIYN
    AIVKVVVANIGYEGQYHVRIVNQDIKTKDDDTSQNNTSEPLNVQTGQENKVSATDTAENSSTATNPKDAS
    DKADVIEPESDVVKVTDSNIDKDAHHDVDHLSDMSDNTHLDKYDLKEMDTQIAKDTDKGVDKDADNSVGM
    SSNVDTEKDINKNEGKVIQLAHNTDKNNHTGKAAKLDGVRQNYNNIDKVTDKKTTEHPSNDIHKTVDKTV
    KTKEKSGTPSKENKLSQSKMLPKTGETTSSQTWWGLYALLGMLALFIPKFRKEPK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Clarke et al., 2006)
20. lukF
  • Gene Name : lukF
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : CRL36945
  • Other Database IDs : CDD:273388
  • Taxonomy ID : 1280
  • Protein Name : leukotoxin LukF
  • Protein pI : 9.51
  • Protein Weight : 36115.25
  • Protein Length : 385
  • Protein Note : beta-channel forming cytolysin; TIGR01002
  • Protein Sequence : Show Sequence
    >CRL36945.1 leukotoxin LukF [Staphylococcus aureus]
    MKMKKLVKSSIASSIALILLSNTVDAAQHITPVSEKKVDDKITLYKTTATSDNDKLNLSQILIFNFIKDK
    SYDKDTLILKAAGNINSGYKKPNPKDYNYSHFYWGGKYNVSISSESNENVNVVDYAPKNQNEEFQVQQTL
    GYSYGGDINISNGLSGGGNGSKSFSETINYKQESYRTTIDRKTNHKFIGWGVEAHKIMNNGWGPYGRDSH
    EDTYGNELFLGSRQSSSNAGQNFMPTHQMPLLSRGNFNPEFISVLSHKQNDTKKSKIKVTYQREMDRYTN
    QWNGLHWIGNNYKNQNTVTFTSTYEVDWQNHSVKLLGTDSKETNPGV
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Karauzum et al., 2013)
21. lukS
  • Gene Name : lukS
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : CAA51251
  • Other Database IDs : CDD:285242
    GOA:Q53746
    HSSP: 1PVL
    InterPro: IPR001340
    InterPro: IPR003963
    InterPro: IPR016183
    UniProtKB/TrEMBL: Q53746
  • Taxonomy ID : 1280
  • Protein Name : leucocidin S
  • Protein pI : 9.34
  • Protein Weight : 33903.95
  • Protein Length : 359
  • Protein Note : Leukocidin/Hemolysin toxin family; pfam07968
  • Protein Sequence : Show Sequence
    >CAA51251.1 lukS [Staphylococcus aureus]
    MVKKRLLAATLSLGIITPIATSFHESKADNNIENIGDCAEVVKRTEDTSCDKWGVTQNIQFDFVKDKKYN
    KDALILKMQGFINSKTTYYNYKNTDHIKAMRWPFQYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYN
    IGGNFNSGPSTGGNGSFNYSKTISYNQQNYISEVEHQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYK
    PYSQNPRDYFVPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGNSYLE
    GSRIHKAFVNRNYTVKYEVNWKTHEIKVKGHN
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Karauzum et al., 2013)
22. MecA
  • Gene Name : MecA
  • Sequence Strain (Species/Organism) : Staphylococcus aureus subsp. aureus MRSA252
  • VO ID : VO_0012391
  • NCBI Gene ID : 2861157
  • NCBI Protein GI : 49482291
  • Locus Tag : SAR0039
  • Genbank Accession : BX571856
  • Protein Accession : YP_039515
  • 3D structure: PDB ID : 1VQQ
  • Taxonomy ID : 282458
  • Gene Starting Position : 44918
  • Gene Ending Position : 46924
  • Gene Strand (Orientation) : -
  • Protein Name : penicillin-binding protein 2 prime
  • Protein pI : 9.19
  • Protein Weight : 72330.63
  • Protein Length : 668
  • Protein Note : Identical to Staphylococcus epidermidis, and Staphylococcus aureus penicillin-binding protein 2 prime MecA TR:Q54113 (EMBL: X52592) (668 aa) fasta scores: E(): 0, 100.000% id in 668 aa, and to Staphylococcus sciuri methicillin resistanc protein MecA2 TR:O54283 (EMBL: Y13095) (668 aa) fasta scores: E(): 0, 99.102% id in 668 aa
  • DNA Sequence : Show Sequence
    >NC_002952.2:44918-46924 Staphylococcus aureus subsp. aureus strain MRSA252, complete genome
    GTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTT
    GGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTA
    TCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAG
    TACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCTTTATGTGTTTTATTTACGACTTG
    TTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTC
    GTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCATTATTTTCTAATGCGCTATAGATTGAAAGGATCT
    GTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATT
    TTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTC
    ATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATT
    CTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTTCATATCTTGTAACGTTGTAACCACCCCAAGA
    TTTATCTTTTTGCCAACCTTTACCATCGATTTTATAACTTGTTTTATCGTCTAATGTTTTGTTATTTAAC
    CCAATCATTGCTGTTAATATTTTTTGAGTTGAACCTGGTGAAGTTGTAATCTGGAACTTGTTGAGCAGAG
    GTTCTTTTTTATCTTCGGTTAATTTATTATATTCTTCGTTACTCATGCCATACATAAATGGATAGACGTC
    ATATGAAGGTGTGCTTACAAGTGCTAATAATTCACCTGTTTGAGGGTGGATAGCAGTACCTGAGCCATAA
    TCATTTTTCATGTTGTTATAAATACTCTTTTGAACTTTAGCATCAATAGTTAGTTGAATATCTTTGCCAT
    CTTTTTTCTTTTTCTCTATTAATGTATGTGCGATTGTATTGCTATTATCGTCAACGATTGTGACACGATA
    GCCATCTTCATGTTGGAGCTTTTTATCGTAAAGTTTTTCGAGTCCCTTTTTACCAATAACTGCATCATCT
    TTATAGCCTTTATATTCTTTTTGTTTTAATTCTTCAGAGTTAATGGGACCAACATAACCTAATAGATGTG
    AAGTCGCTTTTCCTAGAGGATAGTTACGACTTTCTGTTTCATTAGTTGTAAGATGAAATTTTTTTGCGAA
    ATCACTTAAATATTCATCCATTTTTTTAACGGTTTTAAGTGGAACGAAGGTATCATCTTGTACCCAATTT
    TGATCCATTTGTTGTTTGATATAGTCTTCAGAAATACTTAGTTCTTTAGCGATTGCTTTATAATCTTTTT
    TAGATACATTCTTTGGAACGATGCCTATCTCATATGCTGTTCCTGTATTGGCCAATTCCACATTGTTTCG
    GTCTAAAATTTTACCACGTTCTGATTTTAAATTTTCAATATGTATGCTTTGGTCTTTCTGCATTCCTGGA
    ATAATGACGCTATGATCCCAATCTAACTTCCACATACCATCTTCTTTAACAAAATTAAATTGAACGTTGC
    GATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTT
    TTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCA
    GTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTT
    TATCTTCAATTGCATCAATAGTATTATTAATTTCTTTATCTTTTGAAGCATAAAAATATATACCAAACCC
    GACAACTACAACTATTAAAATAAGTGGAACAATTTTTATCTTTTTCA
    
    
  • Protein Sequence : Show Sequence
    >YP_039515.1 penicillin-binding protein 2 prime [Staphylococcus aureus subsp. aureus MRSA252]
    MKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFKQVYKDSSYISKSDNGEVEMTERPIKIY
    NSLGVKDINIQDRKIKKVSKNKKRVDAQYKIKTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQ
    SIHIENLKSERGKILDRNNVELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDT
    FVPLKTVKKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDDAVIGK
    KGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNNMKNDYGSGTAI
    HPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPLLNKFQITTSPGSTQKILTAMIGLNNKTLD
    DKTSYKIDGKGWQKDKSWGGYNVTRYEVVNGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGE
    DIPSDYPFYNAQISNKNLDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKK
    NIISKENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKDNPNMMMA
    INVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : An internal region from the transpeptidase domain from the PBP2a (encoded by mecA) was cloned into a mammalian expression vector, to be used as DNA vaccine in a Murine model. After three sets of DNA vaccination, the immune response represented by antibodies against a fragment of PBP2a was evaluated by enzyme linked immunosorbent assay (ELISA), showing a significant antibody response. The antibacterial effect of the DNA vaccine was evaluated by intraperitoneal immunization and challenge with a sublethal dose of MRSA for 7 days in mice. After the challenge, the number of bacteria from kidneys from immunized and non-immunized mice were determined. Kidneys from immunized mice had 1000 times less on bacteria than the positive controls (non-immunized mice) (Senna et al., 2003).
  • Related Vaccine(s): S. aureus DNA Vaccine encoding PBP2a Protein
23. mntC
  • Gene Name : mntC
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : AAL50778
  • Other Database IDs : CDD:320865
    CDD:238347
  • Taxonomy ID : 1280
  • Protein Name : MntC
  • Protein pI : 9.02
  • Protein Weight : 33762.1
  • Protein Length : 359
  • Protein Note : Helical backbone metal receptor (TroA-like domain). These proteins have been shown to function in the ABC transport of ferric siderophores and metal ions such as Mn2+, Fe3+, Cu2+ and/or Zn2+. Their ligand binding site is formed in the interface between...; cl00262
  • Protein Sequence : Show Sequence
    >AAL50778.1 MntC [Staphylococcus aureus]
    MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYDMAKNVGGDNVDIHSIVPVGQDPHEYE
    VKPKDIKKLTDADVILYNGLNLETGNGWFEKALEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPH
    AWLSLDNGIKYVKTIQQTFIDNDKKHKADYEKQGNKYIAQLEKLNNDSKDSKDKFNDIPKEQRAMITSEG
    AFKYFSKQYGITPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFGE
    VYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Yang et al., 2016)
24. sasA
  • Gene Name : sasA
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : AAR15189
  • Other Database IDs : CDD:301310
  • Taxonomy ID : 1280
  • Protein Name : surface protein SasA
  • Protein pI : 10.47
  • Protein Weight : 15925.95
  • Protein Length : 224
  • Protein Note : legume lectins; cl14058
  • Protein Sequence : Show Sequence
    >AAR15189.1 surface protein SasA, partial [Staphylococcus aureus]
    TKSVTSTSSTEQPINTSTNQSTASNNTSQSTTPTSANLNKTSTTSTSTAPVKLRTFSRLAMSTFASAATT
    TALTANTITVNKDNLKQYMTTSGNATYDQSTGVVTLTQDTTSQKGAITLGTRIDSNKSFHFSGKVNLGNK
    YEGHGNGGDGIGFA
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Yang et al., 2017)
25. sdrD
  • Gene Name : sdrD
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : CAA06651
  • Other Database IDs : CDD:309682
    CDD:313621
    CDD:316170
    CDD:319228
    CDD:307064
    GOA:O86488
    InterPro: IPR001899
    InterPro: IPR003367
    InterPro: IPR005877
    InterPro: IPR008454
    InterPro: IPR008966
    UniProtKB/TrEMBL: O86488
  • Taxonomy ID : 426430
  • Protein Name : SdrD
  • Protein pI : 4.04
  • Protein Weight : 134390.88
  • Protein Length : 1376
  • Protein Note : YSIRK type signal peptide; pfam04650
  • Protein Sequence : Show Sequence
    >CAA06651.1 sdrD [Staphylococcus aureus]
    MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFGLGNQEAKAAESTNKELNEATTSASDN
    QSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNETTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKST
    NEDLNTKQTISNQEALQPDLQENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSN
    NENNADIILPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLSLKSQ
    ITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTANNLITYTFTDYVDRFN
    SVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQYPDYVVNEKNSIGSAFTETVSHVGNKEN
    PGYYKQTIYVNPSENSLTNAKLKVQAYHSSYPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDV
    TNQYLQKITYGDNNSAVIDFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNN
    QSGGAGQEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPNLPNGDY
    RVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKYNLGDYVWEDTNKNGIQDQ
    DEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGNYKVEFTTPEGYTPTTVTSGSDIEKDSNGLT
    TTGVINGADNMTLDSGFYKTPKYNLGNYVWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDG
    KYQFTGLENGTYKVEFETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVW
    EDTNKNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGYTPTSVTSG
    NDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVKVTLLNEKGE
    VIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLTQTGTNTTEDDKDADGGEVDVTITDHDDFTLDNGYYE
    EETSDSDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSDS
    DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHT
    PVKPMSTTKDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKKQNK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Rauch et al., 2014)
26. seb
  • Gene Name : seb
  • Sequence Strain (Species/Organism) : Staphylococcus aureus subsp. aureus COL
  • NCBI Protein GI : AAL04126
  • Other Database IDs : CDD:279467
    CDD:280949
  • Taxonomy ID : 93062
  • Protein Name : Seb
  • Protein pI : 8.81
  • Protein Weight : 30196.07
  • Protein Length : 329
  • Protein Note : pathogenicity island 3
  • Protein Sequence : Show Sequence
    >AAL04126.1 Seb [Staphylococcus aureus subsp. aureus COL]
    MYKRLFISHVILIFALILVISTPNVLAESQPDPKPDELHKSSKFTGLMENMKVLYDDNHVSAINVKSIDQ
    FLYFDLIYSIKDTKLGNYDNVRVEFKNKDLADKYKDKYVDVFGANYYYQCYFSKKTNDINSHQTDKRKTC
    MYGGVTEHNGNQLDKYRSITVRVFEDGKNLLSFDVQTNKKKVTAQELDYLTRHYLVKNKKLYEFNNSPYE
    TGYIKFIENENSFWYDMMPAPGDKFDQSKYLMMYNDNKMVDSKDVKIEVYLTTKKK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Zhao et al., 2014)
27. spa
  • Gene Name : spa
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : P38507
  • Other Database IDs : CDD:309682
    CDD:280394
    CDD:331228
    CDD:212030
  • Taxonomy ID : 1280
  • Gene Strand (Orientation) : ?
  • Protein Name : Immunoglobulin G-binding protein A
  • Protein pI : 5.51
  • Protein Weight : 56697.01
  • Protein Length : 687
  • Protein Note : IgG-binding protein A; Staphylococcal protein A; SpA
  • Protein Sequence : Show Sequence
    >sp|P38507.1|SPA_STAAU RecName: Full=Immunoglobulin G-binding protein A; Short=IgG-binding protein A; AltName: Full=Staphylococcal protein A; Short=SpA; Flags: Precursor
    MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQNAFYQVLNMPNLNADQRNGFIQSLKD
    DPSQSANVLGEAQKLNDSQAPKADAQQNKFNKDQQSAFYEILNMPNLNEEQRNGFIQSLKDDPSQSTNVL
    GEAKKLNESQAPKADNNFNKEQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNESQAP
    KADNKFNKEQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNKEQQN
    AFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKPGKEDGNKPGKEDGNKP
    GKEDNKKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDGNKPGKEDGNKPGKEDGNKPGKEDGNGVHVVKPG
    DTVNDIAKANGTTADKIAADNKLADKNMIKPGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFG
    GLSLALGAALLAGRRREL
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Pozzi et al., 2015)
28. staphylococcal enterotoxin A
  • Gene Name : staphylococcal enterotoxin A
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : AAA26681
  • Other Database IDs : CDD:279467
    CDD:280949
  • Taxonomy ID : 1280
  • Protein Name : staphylococcal enterotoxin A
  • Protein pI : 8.15
  • Protein Weight : 28651.08
  • Protein Length : 337
  • Protein Note : staphylococcal enterotoxin A signal peptide
  • Protein Sequence : Show Sequence
    >AAA26681.1 staphylococcal enterotoxin A precursor [Staphylococcus aureus]
    MKKTAFTLLLFIALTLTTSPLVNGSEKSEEINEKDLRKKSELQGTALGNLKQIYYYNEKAKTENKESHDQ
    FLQHTILFKGFFTDHSWYNDLLVDFDSKDIVDKYKGKKVDLYGAYYGYQCAGGTPNKTACMYGGVTLHDN
    NRLTEEKKVPINLWLDGKQNTVPLETVKTNKKNVTVQELDLQARRYLQEKYNLYNSDVFDGKVQRGLIVF
    HTSTEPSVNYDLFGAQGQYSNTLLRIYRDNKTINSENMHIDIYLYTS
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Reddy et al., 2015)
29. traP
  • Gene Name : traP
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : ADK12000
  • Other Database IDs : CDD:292771
  • Taxonomy ID : 1280
  • Protein Name : target of RNAIII-activating protein
  • Protein pI : 7.23
  • Protein Weight : 20101.19
  • Protein Length : 243
  • Protein Note : TraP
  • Protein Sequence : Show Sequence
    >ADK12000.1 target of RNAIII-activating protein [Staphylococcus aureus]
    MKKLYTSYGTYGFLNQIKINNPSHHLFQFSTADSSVIFEETEEKTVLKSPSIYEVIKEIGAFNEDHFYCA
    IFIPSTEDHVYQLEKKLISVDDNFKNFGGFKSYRLLRPVKGTTYKIYFGFADRQTYEDFKNSDAFKDHFS
    KEALSHYFGSSGQHSSYFERYLYPIKE
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Yu et al., 2014)
30. TSST-1
  • Gene Name : TSST-1
  • Sequence Strain (Species/Organism) : Staphylococcus aureus
  • NCBI Protein GI : AAA26682
  • Other Database IDs : CDD:332684
  • Taxonomy ID : 1280
  • Protein Name : toxic shock syndrome toxin-1
  • Protein pI : 9.34
  • Protein Weight : 25910.55
  • Protein Length : 314
  • Protein Note : toxic shock syndrome toxin-1 signal peptide
  • Protein Sequence : Show Sequence
    >AAA26682.1 toxic shock syndrome toxin-1 precursor [Staphylococcus aureus]
    MNKKLLMNFFIVSPLLLATTATDFTPVPLSSNQIIKTAKASTNDNIKDLLDWYSSGSDTFTNSEVLDNSL
    GSMRIKNTDGSISLIIFPSPYYSPAFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIEL
    PLKVKVHGKDSPLKYGPKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSK
    KFEYNTEKPPINIDEIKTIEAEIN
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Narita et al., 2015)
1. Ifng (Interferon gamma)
  • Gene Name : Ifng (Interferon gamma)
  • Sequence Strain (Species/Organism) : Mouse
  • NCBI Gene ID : 15978
  • NCBI Protein GI : 33468859
  • Genbank Accession : NM_008337
  • Protein Accession : NP_032363.1
  • Other Database IDs : MGI:107656; UniProt: P01580
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • DNA Sequence : Show Sequence
    >gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
    ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
    GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
    GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
    AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
    AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
    GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
    AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
    GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
    AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
    AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
    AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
    TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
    TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
    ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
    GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
    AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
    TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
    AGTATACCTTTGCAACC
  • Protein Sequence : Show Sequence
    >gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
    MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
    ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
    LLPESSLRKRKRSRC
  • Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
  • Additional Molecule Role : Vaximmutor
  • Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
  • Related Vaccine(s): Staphylococcus aureus aroA mutant vaccine
2. Il4 (interleukin 4)
  • Gene Name : Il4 (interleukin 4)
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16189
  • NCBI Protein GI : 10946584
  • Locus Tag : RP23-188H3.4
  • Genbank Accession : NM_021283.1
  • Protein Accession : NM_021283.1
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • Protein Name : IL-4, Interleukin 4
  • DNA Sequence : Show Sequence
    >gi|10946583|ref|NM_021283.1| Mus musculus interleukin 4 (Il4), mRNA
    GGATCCCCGGGCAGAGCTGGGGGGGGATTTGTTAGCATCTCTTGATAAACTTAATTGTCTCTCGTCACTG
    ACGGCACAGAGCTATTGATGGGTCTCAACCCCCAGCTAGTTGTCATCCTGCTCTTCTTTCTCGAATGTAC
    CAGGAGCCATATCCACGGATGCGACAAAAATCACTTGAGAGAGATCATCGGCATTTTGAACGAGGTCACA
    GGAGAAGGGACGCCATGCACGGAGATGGATGTGCCAAACGTCCTCACAGCAACGAAGAACACCACAGAGA
    GTGAGCTCGTCTGTAGGGCTTCCAAGGTGCTTCGCATATTTTATTTAAAACATGGGAAAACTCCATGCTT
    GAAGAAGAACTCTAGTGTTCTCATGGAGCTGCAGAGACTCTTTCGGGCTTTTCGATGCCTGGATTCATCG
    ATAAGCTGCACCATGAATGAGTCCAAGTCCACATCACTGAAAGACTTCCTGGAAAGCCTAAAGAGCATCA
    TGCAAATGGATTACTCGTAGTACTGAGCCACCATGCTTTAACTTATGAATTTTTAATGGTTTTATTTTAA
    TATTTATATATTTATAATTCATAAAATAAAATATTTGTATAATGT
  • Protein Sequence : Show Sequence
    >gi|10946584|ref|NP_067258.1| interleukin 4 [Mus musculus]
    MGLNPQLVVILLFFLECTRSHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCR
    ASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS
  • Molecule Role : Vaximmutor
  • Molecule Role Annotation : IL-4 plays an important role in Th2 immune response.
  • Related Vaccine(s): Staphylococcus aureus aroA mutant vaccine
IV. Vaccine Information
1. S. aureus ClfA Protein Vaccine
a. Vaccine Ontology ID:
VO_0004047
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
Recombinant ClfA protein (Josefsson et al., 2001).
e. Gene Engineering of ClfA
  • Type: Recombinant protein preparation
  • Description: Expression and purification of recombinant proteins.Vector pCF40 expressed rClfA truncate rClfA40‐559 (Josefsson et al., 2001).
  • Detailed Gene Information: Click here.
f. Adjuvant: complete Freunds adjuvant
g. Immunization Route
subcutaneous injection
h. Mouse Response
  • Host Strain: NMRI
  • Vaccination Protocol: Purified rClfA region A, comprising residues 40–559 (rClfA40‐559), and BSA (Sigma Chemical) were dissolved in physiologic saline and were emulsified 1:1 in Freund's complete adjuvant (Difco Laboratories). In total, 200 μL of the emulsion containing 30 μg of protein was injected subcutaneously (sc) on day −21 ( /group). Booster immunization with 30 μg of protein in physiologic saline:Freund's incomplete adjuvant 1:1 sc was done on day −10 (Josefsson et al., 2001).
  • Challenge Protocol: On day 0, the mice were challenged iv with 1.6 x 107 cfu/mouse of wild‐type S. aureus strain Newman (Josefsson et al., 2001).
  • Efficacy: Mice immunized with rClfA40‐559 had less severe arthritis than did the control BSA‐immunized group throughout the experiment. Both the severity of synovitis and the extent of joint destruction were markedly less pronounced in mice immunized with rClfA40‐559 than in the BSA control group (Josefsson et al., 2001).
2. S. aureus CNA Protein Vaccine
a. Vaccine Ontology ID:
VO_0004048
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
a recombinant fragment of CNA protein (Nilsson et al., 1998).
e. Gene Engineering of cna
  • Type: Recombinant protein preparation
  • Description: CNA fragments were expressed in E. coli and then purified (Nilsson et al., 1998).
  • Detailed Gene Information: Click here.
f. Adjuvant: complete Freunds adjuvant
g. Immunization Route
subcutaneous injection
h. Mouse Response
  • Host Strain: NMRI
  • Vaccination Protocol: Purified recombinant collagen adhesin fragments M17, M31, M55, and BSA (Sigma Chemical Co.) were dissolved in PBS and emulsified 1:1 in Freund’s complete adjuvant 200 μl of the emulsion containing 100 μg of protein was injected subcutaneously (s.c.) on day -31. Booster immunizations (s.c.; 100 μg protein in PBS) were performed 15 and 24 d after the initial vaccination (Nilsson et al., 1998).
  • Challenge Protocol: After the final boost, mice were inoculated intravenously (i.v.) with S. aureus (Nilsson et al., 1998).
  • Efficacy: At 14 d after inoculation, mortality in the collagen adhesin-vaccinated group was only 13%, compared with 87% in the control antigen immunized group (Nilsson et al., 1998).
3. S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna
a. Vaccine Ontology ID:
VO_0004542
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Antigen
fibrinogen binding protein (Efb), fibronectin-binding protein A (FnbpA), clumping factor A (ClfA) and collagen-binding protein (Cna) from S. aureus strain 1706 (Castagliuolo et al., 2006)
f. Gene Engineering of ClfA
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
g. Gene Engineering of cna
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
h. Gene Engineering of efb
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
i. Gene Engineering of fnbA
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
j. Vector:
pcDNA3.1 (Castagliuolo et al., 2006)
k. Immunization Route
intranasal immunization
l. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: Intranasal immunization with a pDNA mixture coding the four adhesins triggered significant levels of specific serum and mucosal Ig that inhibited S. aureus adhesion to cow mammary gland epithelial cells in vitro. Splenocytes of immunized mice challenged in vitro with S. aureus extracts showed a strong proliferative response (Castagliuolo et al., 2006).
  • Efficacy: In non immune mice or mice injected with empty vectors, a large number of bacteria was recovered from the mammary glands. Whereas, in mice immunized with the genetic vaccine coding the four S. aureus adhesins, the amount of bacteria recovered from the mammary glands was significantly decreased (Castagliuolo et al., 2006).
4. S. aureus DNA Vaccine encoding PBP2a Protein
a. Vaccine Ontology ID:
VO_0004192
b. Type:
DNA vaccine
c. Status:
Research
d. Gene Engineering of MecA
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
e. Vector:
pCI-Neo Mammalian Expression Vector (Promega)
f. Immunization Route
Intramuscular injection (i.m.)
g. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Three days before the first immunization, bupivacain chloridrate 0.5% was injected at a dose of 2.5 μl/g of animal weight in the left quadriceps muscle. For DNA vaccination, three intramuscular injections with 10 μg of plasmid pCI-Neo-mecA or pCI-Neo only (negative control) diluted in 50 μl of sterile PBS were given at 2 weeks intervals into the left quadriceps muscle of mice (Senna et al., 2003).
  • Challenge Protocol: To assess the immune response against MRSA, mice immunized with pCI-Neo and pCI-Neo-mecA were challenged with MRSA (MRSA HPS-03). Mice received a sublethal dose of 2.0×106 cfu intraperitonially daily for 7 days. A non-immunized group receiving the same bacterial dose was included as positive control.
  • Efficacy: After challenge, the number of bacteria from kidneys from immunized and non-immunized mice was determined. Kidneys from immunized mice had 1000 times less on bacteria than the positive controls (non-immunized mice) (Senna et al., 2003).
5. S. aureus DNA vaccine pClfaSrtD13
a. Vaccine Ontology ID:
VO_0004541
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Antigen
Clumping factor A (Clfa), fibronectin binding protein A (FnBPA) and the enzyme Sortase (Srt) from strains “Newman” or “8325-4” (Gaudreau et al., 2007)
f. Gene Engineering of ClfA
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
g. Vector:
pCI (Gaudreau et al., 2007)
h. Immunization Route
Intramuscular injection (i.m.)
i. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: All animals produced a mixed Th1 and Th2 response including functional antigen-specific, mostly IgG2a antibodies, sustained production of IFN-gamma and a predominantly CD8+ T-cell response (Gaudreau et al., 2007).
  • Efficacy: Fourteen days after challenge, 70% of the vaccinated mice had survived as compared to only 15% of the control mice and after 21 days, the figures were 55% survival for the vaccinated as compared to 15% survival of the control group. However, signs of arthritis were observed in all the infected mice whether vaccinated or not (Gaudreau et al., 2007).
6. S. aureus FnbA Protein Vaccine
a. Vaccine Ontology ID:
VO_0004049
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
Fibronectin binding domain of FnbA protein
e. Gene Engineering of fnbA
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant: complete Freunds adjuvant
g. Immunization Route
subcutaneous injection
h. Mouse Response
  • Host Strain: BSVS
  • Vaccination Protocol: Mice were vaccinated subcutaneously in the neck region with 20 μg of the antigens emulsified with complete Freund's adjuvant (primary vaccination) a few days after mating. Booster doses were given after 14 days with the same amount of immunogen, but emulsified with incomplete Freund's adjuvant (Mamo et al., 1994).
  • Challenge Protocol: Mice were challenged 18-21 days after the booster dose by intramammary inoculation (mouse mastitis model) 23. Briefly, mice were anaesthetized using ether inhalation, the teat tips were aseptically removed and a 0.1 ml bacterial suspension (1 x l0^6 c.f.u, m1-1) was inoculated into the left quarter (L-4) and right quarter (R-4) mouse mammary glands (Mamo et al., 1994).
  • Efficacy: Vaccination of mice with recombinant FnBP resulted in significant protection against challenge with S. aureus (Mamo et al., 1994).
7. S. aureus IsdB subunit vaccine (V710)
a. Product Name:
V710
b. Vaccine Ontology ID:
VO_0004220
c. Type:
Subunit vaccine
d. Status:
Research
e. Antigen
Purified IsdB protein
f. Adjuvant: amorphous aluminum hydroxyphosphate sulfate adjuvant (AAHSA)
g. Immunization Route
Intramuscular injection (i.m.)
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Groups of 20 BALB/c or ICR mice were immunized three times with IsdB (20 μg per dose) formulated with amorphous aluminum hydroxyphosphate sulfate adjuvant (AAHSA) (450 μg per dose) or were sham immunized with AAHSA alone. The doses were administered as two 50-μl intramuscular injections on days 0, 7, and 21(Kuklin et al., 2006).
  • Challenge Protocol: 35 days after vaccination, mice were challenged with an 80 to 90% lethal dose (LD80-90) of S. aureus Becker (4.9 × 108 to 8.7 × 108 CFU for BALB/c mice and 1.0 × 109 to 2.0 × 109 CFU for ICR mice), and survival was monitored for 10 days. (Kuklin et al., 2006).
  • Efficacy: Mice immunized with IsdB exhibited greater survival (45, 40, 32, 29, 40, and 40%) than the mice in the sham-immunized control group (Kuklin et al., 2006).
8. Staphylococcus aureus aroA mutant vaccine
a. Vaccine Ontology ID:
VO_0002797
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of aroA
f. Immunization Route
Intramammary immunization
g. Mouse Response
  • Persistence: An aroA mutant is attenuated in mice (Buzzola et al., 2006).
  • Efficacy: An aroA mutant induces significant protection in mice from challenge with wild type S. aureus (Buzzola et al., 2006).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: Ninety-six hours after challenge with the wild-type strain RN6390 or heterologous strain MB319, the relative mRNA levels of IFN-γ and IL-4 were determined in mammary glands by RT-PCR. Mammary glands from mice immunized with the aroA mutant showed a significant increase in the level of IFN-γ transcripts compared with control, unvaccinated mice (Buzzola et al., 2006).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Il4 (interleukin 4)
    • Gene Response: Ninety-six hours after challenge with the wild-type strain RN6390 or heterologous strain MB319, the relative mRNA levels of IFN-γ and IL-4 were determined in mammary glands by RT-PCR. Mice immunized with the aroA mutant showed a significant increase in the level of IL-4 transcripts compared with control, unvaccinated mice (Buzzola et al., 2006).
    • Detailed Gene Information: Click here.
V. References
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13. Lacey et al., 2017: Lacey KA, Leech JM, Lalor SJ, McCormack N, Geoghegan JA, McLoughlin RM. The Staphylococcus aureus Cell Wall-Anchored Protein Clumping Factor A Is an Important T Cell Antigen. Infection and immunity. 2017; 85(12); . [PubMed: 28947645].
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19. Pozzi et al., 2015: Pozzi C, Lofano G, Mancini F, Soldaini E, Speziale P, De Gregorio E, Rappuoli R, Bertholet S, Grandi G, Bagnoli F. Phagocyte subsets and lymphocyte clonal deletion behind ineffective immune response to Staphylococcus aureus. FEMS microbiology reviews. 2015; 39(5); 750-763. [PubMed: 25994610].
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26. Yang et al., 2016: Yang HJ, Zhang JY, Wei C, Yang LY, Zuo QF, Zhuang Y, Feng YJ, Srinivas S, Zeng H, Zou QM. Immunisation With Immunodominant Linear B Cell Epitopes Vaccine of Manganese Transport Protein C Confers Protection against Staphylococcus aureus Infection. PloS one. 2016; 11(2); e0149638. [PubMed: 26895191].
27. Yang et al., 2017: Yang Y, Yu R, Yang X, Liu S, Fang T, Song X, Hou L, Yu C, Xu J, Fu L, Yi S, Chen W. Protection against Staphylococcus aureus and tetanus infections by a combined vaccine containing SasA and TeNT?Hc in mice. Molecular medicine reports. 2017; 15(4); 2369-2373. [PubMed: 28259925].
28. Yu et al., 2014: Yu L, Fan Z, Ma J, Tong C, Song B, Zhu Z, Cui Y. Cross-protective effect of a novel multi-antigen-chimeric vaccine against Streptococcus and Staphylococcus aureus infection in mice. Journal of medical microbiology. 2014; 63(Pt 12); 1732-1740. [PubMed: 25288644].
29. Yu et al., 2015: Yu S, Zhang H, Yao D, Liu W, Wang X, Chen X, Wei Y, Zhang Z, Wang J, Yu L, Sun H, Wu Z, Yu Y, Song B, Ma J, Tong C, Cui Y. Identification of CD4+ T-cell epitopes on iron-regulated surface determinant B of Staphylococcus aureus. Microbial pathogenesis. 2015; 89; 108-113. [PubMed: 26423555].
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