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Streptococcus agalactiae

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Disease
    3. Introduction
    4. Microbial Pathogenesis
    5. Host Ranges and Animal Models
    6. Host Protective Immunity
  2. Vaccine Related Pathogen Genes
    1. 5'-nucleotidase family protein (Protective antigen)
    2. aldehyde dehydrogenase (Protective antigen)
    3. alp1 (Protective antigen)
    4. alp3 (Protective antigen)
    5. argF (Protective antigen)
    6. bac (Protective antigen)
    7. bibA (Protective antigen)
    8. BipA (Protective antigen)
    9. Branched-chain alpha-keto acid dehydrogenase subunit E2 (Protective antigen)
    10. C protein alpha-antigen (Protective antigen)
    11. eno (Protective antigen)
    12. immunogenic secreted protein (Protective antigen)
    13. pcsB (Protective antigen)
    14. peptidase M15 (Protective antigen)
    15. rib (Protective antigen)
    16. sar5 (Protective antigen)
    17. scpA_4 (Protective antigen)
    18. scpB (Protective antigen)
    19. Sip (Protective antigen)
    20. srr-1 (Protective antigen)
    21. tig (Protective antigen)
    22. tuf (Protective antigen)
  3. Vaccine Related Host Genes
    1. IgG
  4. Vaccine Information
    1. S. agalactiae BipA Protein Vaccine
    2. S. agalactiae BPS Protein Vaccine
    3. S. agalactiae Rib Protein Vaccine
    4. S. agalactiae Sip Protein Vaccine
  5. References
I. General Information
1. NCBI Taxonomy ID:
1311
2. Disease:
Group B streptococcus (GBS) infection
3. Introduction
S. agalactiae is a member of the gastrointestinal normal flora in some humans and can spread to secondary sites - including the vagina in 10-30% of women. This is of clinical importance: S. agalactiae can be transferred to a neonate passing through the birth canal and can cause serious group B streptococcal infection. In the western world, S. agalactiae is the main cause of bacterial septicemia of the newborn, which can lead to death or long-term sequelae. S. agalactiae invade via alveolar and pulmonary epithelial cells; newborns are especially succeptible to infection because they lack alveolar macrophages to prevent invasion. Newborn GBS disease is separated into early-onset disease occurring on living days 0-7 and late-onset disease which starts on days 7-90. Early-onset septicemia is more prone to be accompanied by pneumonia, while late-onset septicimia is more often accompanied by meningitis. S. agalactiae neonatal meningitis does often not present with the hallmark sign of adult meningitis, a stiff neck; rather, it presents with nonspecific symptoms such as fever, vomiting and irritability and can consequently lead to late diagnosis. Hearing loss can be a long-term sequelae of GBS-meningitis. Infection with GBS is the cause of some instances of stillbirth (Wiki: S. agalactiae).
4. Microbial Pathogenesis
Experimental study of early-onset infection suggests that this bacteria to invade fetal epithelial and endothelial cells and certain macrophages and the mortality rates for early-onset is 4-6%. This invasion is confirmed to play important role in its pathogenesis through the ability of S. agalactiae in the monkey model. Ability to invade and transcytose, this bacteria can enter into the respiratory tract causing pneumonia or further into the blood causing septicemia. Bloodstream enables this bacteria to reach different sites of body causing meningitis and osteomyelitis (MicrobeWiki: S. agalactiae).
5. Host Ranges and Animal Models
S. agalactiae colonizes in the body of some animals, including cow, sheep, and humans without causing any harm. The habitat of this microorganism is largely confined to the intestine and vagina in human and the mammary gland of cows and sheep. This microorganism also colonizes in the genital and/or intestinal tract of about 10-30% of pregnant women. However, some can actually cause diseases in their neonates or immunocompromised mammals. Monkeys and mice can be used as a model of disease (MicrobeWiki: S. agalactiae).
6. Host Protective Immunity
Passive immunization with antibodies has been shown to be protective in mice (Erdogan et al., 2002).
1. 5'-nucleotidase family protein
  • Gene Name : 5'-nucleotidase family protein
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : ASZ01629
  • Other Database IDs : CDD:326311
    CDD:277317
    CDD:308488
  • Taxonomy ID : 1311
  • Protein Name : 5'-nucleotidase family protein
  • Protein pI : 8.94
  • Protein Weight : 69797.41
  • Protein Length : 771
  • Protein Note : metallophosphatase superfamily, metallophosphatase domain; cl13995
  • Protein Sequence : Show Sequence
    >ASZ01629.1 5'-nucleotidase family protein [Streptococcus agalactiae]
    MKKKIILKSSVLGLVAGTSIMFSSVFADQVGVQVIGVNDFHGALDNTGTANMPDGKVTNAGTAAQLDAYM
    DDAQKDFKQTNPNGESIRVQAGDMVGASPANSGLLQDEPTVKTFNAMNVEYGTLGNHEFDEGLAEYNRIV
    TGKAPAPDSNINNITKSYPHEAAKQEIVVANVIDKVNKQIPYNWKPYTIKNIPVNNKSVNVGFIGIVTKD
    IPNLVLRKNYEQYEFLDEAETIVKYAKELQAKNVKAIVVLAHVPATSKDDIAEGEAAEMMKKVNQLFPEN
    SVDIVFAGHNHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAKVIAVAPGKKTGSADIQ
    AIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDNVSPVGSLITEAQLAIARKSWPDIDFAMTNNGGIRA
    DLLIKPDGTITWGAAQAVQPFGNILQVVEITGRDLYKALNEQYDQKQNFFLQIAGLRYTYTDNKEGGEET
    PFKVVKAYKSNGEEINPDAKYKLVINDFLFGGGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVS
    IPNNKPKIYVTMKMVNETITQNDGTYSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVTTIHKKQ
    LHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIALNTKKKHMK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Liu et al., 2013)
2. aldehyde dehydrogenase
  • Gene Name : aldehyde dehydrogenase
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : OZV86763
  • Taxonomy ID : 1311
  • Protein Name : aldehyde dehydrogenase
  • Protein pI : 4.43
  • Protein Weight : 47418.37
  • Protein Length : 528
  • Protein Note : in Escherichia coli this enzyme appears to be an NAD+/NADP+-dependent succinate semialdehyde dehydrogenase; Derived by automated computational analysis using gene prediction method: Protein Homology.
  • Protein Sequence : Show Sequence
    >OZV86763.1 aldehyde dehydrogenase [Streptococcus agalactiae]
    MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHALYKTWRKEDNVEERQNQLHKVADLLRKDRDKYAEVM
    TKDMGKLFTEAQGEVDLCADIADYYADNGQKFLKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMR
    VFAPNFIVGNTMLLKHASICPASAQAFEDLVREAGAPEGAFKNIFASYDQVSNLISDPRVAGVCLTGSER
    GGASIAAEAGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKYDEFV
    NMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDTIDHPGNFVMPTVLTNI
    TKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTVFSSDPEHAKKVAAQIETGMTFINSGWTS
    LPELPFGGIKNSGYGRELSQLGFDAFVNEHLVFTPNSD
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Brzychczy-Wloch et al., 2013)
3. alp1
  • Gene Name : alp1
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : AAR08144
  • Other Database IDs : CDD:282500
    CDD:204077
    CDD:131384
    CDD:298076
  • Taxonomy ID : 342614
  • Protein Name : alpha-like protein 1
  • Protein pI : 4.79
  • Protein Weight : 38063.88
  • Protein Length : 471
  • Protein Note : YSIRK type signal peptide; pfam04650
  • Protein Sequence : Show Sequence
    >AAR08144.1 alpha-like protein 1, partial [Streptococcus agalactiae 515]
    MFRRSKNNSYDTSQTKQRFSIKKFKFGAASVLIGISFLGGFTQGQFNISTDTVFAAEVISGSAATLNSAL
    VKNVSGGKAYIDIYDVKNGKIDPLNLIVLTPSNYSANYYIKQGGRIFTSVNQLQTPGTATITYNILDENG
    NPYTKSDGQIDIVSLVTTVYDTTELRNNINKVIENANDPKWSDDSRKDVLSKIEVIKNDIDNNPKTQSDI
    DNKIVEVNELEKLLVLPVPDKDKYDPTGGETTVPQGTPVSDKEITDLVKIPDGSKGVPTVVGDRPDTNVP
    GDHKVTVDVTYPDGTKDTVEVTVHVTPKPVPDKDKYDPTGGETTVPQGTPVSDKEITDLVKIPDGSKGVP
    TVVGDRPDTNVPGDHKVTVDVTYPDGTKDTVEVTVHVTPKP
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Kvam et al., 2011)
4. alp3
  • Gene Name : alp3
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : AKI94854
  • Other Database IDs : CDD:309682
    CDD:312387
    CDD:131384
    CDD:307064
  • Taxonomy ID : 1311
  • Protein Name : Alpha-like protein 3
  • Protein pI : 4.78
  • Protein Weight : 62530.04
  • Protein Length : 698
  • Protein Note : YSIRK type signal peptide; pfam04650
  • Protein Sequence : Show Sequence
    >AKI94854.1 Alpha-like protein 3 [Streptococcus agalactiae]
    MFRRSKNNSYDTSQTKQRFSIKKFKFGAASVLIGLSFLSGVTQGNLNIFEESIVAASTIPGSAATLNTSI
    TKNIQNGNAYIDLYDVKNGLIDPQNLIVLNPSSYSANYYIKQGAKYYSNPSEITTTGSATITFNILDETG
    NPHKKADGQIDIVSVNLTIYDSTALRNRIDEVINNANDPKWSDGSRDEVLTGLEKIKKDIDNNPKTQIDI
    DNKINEVNEIEKLLVVSLPDKIKYSPEAKHRTVEQHAELDAKDSIANTDELPSNSTYNWKNGHKPDTSTS
    GEKDGIVEVHYPDGTVDDVNVKVTVTSKKTDNTAPTLTVTPEQQTVKVDEDITFTVTVEDENEVELGLDD
    LKAKYENDIIGARVKIKYLTKESNKKVMEVTIMKATLADKGAITFTAKDKAGNQAEPKTVTINVLPLKDS
    NEPKGKDQTVKVGETPKAEDSIGNLSDLPKGTTVAFETPVDTATPGDKPAKVVVTYPDGSKDTVDVTVKV
    VDPRTDADKNDPAGKDQQVKVGETPKAEDSIGNLSDLPKGTTVAFETPVDTATPGDKPAKVVVTYPDGSK
    DTVDVTVKVVDPRTDADKNDPAGKDQQVNGKGNKLPATGENATPFFNVAALTIISSVGLLSVSKKKED
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Kvam et al., 2011)
5. argF
  • Gene Name : argF
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : AMQ15599
  • Other Database IDs : CDD:235269
  • Taxonomy ID : 1311
  • Protein Name : Ornithine carbamoyltransferase
  • Protein pI : 4.62
  • Protein Weight : 35102
  • Protein Length : 408
  • Protein Note : ornithine carbamoyltransferase; Provisional
  • Protein Sequence : Show Sequence
    >AMQ15599.1 Ornithine carbamoyltransferase [Streptococcus agalactiae]
    MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLVGKNIALIFEKDSTRTRCAFEVAAHDQ
    GAHVTYLGPTGSQMGKKETSKDTARVLGGMYDGIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLA
    DFLTAKECLHKPYKDIRFTYVGDGRNNVANALMIGASIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGA
    SIEITDDIAEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPSFHNI
    DTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Liu et al., 2013)
6. bac
  • Gene Name : bac
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : BAC98831
  • Other Database IDs : CDD:273479
    CDD:301352
    CDD:214653
    CDD:285864
    CDD:293433
    CDD:282865
    CDD:283799
    CDD:279132
  • Taxonomy ID : 1311
  • Protein Name : c protein beta antigen
  • Protein pI : 7.31
  • Protein Weight : 115168.35
  • Protein Length : 1162
  • Protein Note : Gram-positive signal peptide, YSIRK family; TIGR01168
  • Protein Sequence : Show Sequence
    >BAC98831.1 c protein beta antigen [Streptococcus agalactiae]
    MFKSNYERKMRYSIRKFSVGVASVAVASLFMGSVAHASELVKDNSVKTTEVAAKPYPSMAQTDQGNNSSS
    SELETTKMEIPTTDIKKAVEPVEKTAGETSATDTGKREKQLQQWKNNLKNDVDNTILSHEQKNEFKTKID
    ETNDSDALLELENQFNETNRLLHIKQHEEVEKDKKAKQQKTLKQSDTKVDLSNIDKELNHQKSQVEKMAE
    QKGITNEDKDSMLKKIEDIRKQAQQADKKEDAEVKVREELDKATTNEQATQVKNQFLENAQKLKEIQPLI
    KETNVKLYKAMSESLEQVEKELKHNSEANLEDLVAKSKEIVREYEGKLNQSKNLPELKQLEEEAHSKLKQ
    VVEDFRKKFKTSEQVTPKKRVKRDLAANENNQQKIELTVSPENITVYEGEDVKFTVTAKSDSKTTLDFSD
    LLTKYNPSVSDRISTNYKTNTDNHKIAEITIKNLKLNESQTVTLKAKDDSGNVVEKTFTITVQKKEEKQV
    PKTPEQKDSKTEEKVPQEPKSNDKNQLQELIKSAQQELEKLEKAIKELMEQPEIPSNPEYGIQKSIWESQ
    KEPIQEAITSFKKIIGDSSSKYYTEHYFNKYKSDFMNYQLHAQMEMLTRKVVQYMNKYPDNAEIKKIFES
    DMKRTKEDNYGSLENDALKGYFEKYFLTPFNKIKQIVDDLDKKVEQDQPAPIPENSEMDQAKEKAKIAVS
    KYMSKVLDGVHQHLQKKNHSKIVDLFKELEAIKQQTIFDIDNAKTEVEIDNLVHDAFSKMNATVAKFQKG
    LETNTPETPDTPKIPELPQAPDTPQAPDTPHVPESPKAPEAPRVPESPKTPEAPHVPESPKTPEAPKIPE
    PPKTPDVPKLPDVPKLPDAPKLPDGLNKVGQAVFTSTDGNTKVTVVFDKPTDADKLHLKEVTTKELADKI
    AHKTGGGTVRVFDLSLSKGGKETHVNGERTVRLALGQTGSDVHVYHVKENGDLERIPSKVENGQVVFKTN
    HFSLFAIKTLSKDQNVTPPKQTKPSTQGSQVEIAESQTGKFQSKAANHKALATGNETVAKGNPTSTTEKK
    LPYTGVASNLVLEIMGLLGLIGTSFIAMKRRKS
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Yang et al., 2007)
7. bibA
  • Gene Name : bibA
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : AHJ88300
  • Taxonomy ID : 1311
  • Protein Name : BibA
  • Protein pI : 10.47
  • Protein Weight : 42081.88
  • Protein Length : 455
  • Protein Sequence : Show Sequence
    >AHJ88300.1 BibA, partial [Streptococcus agalactiae]
    SDSIPHKKQVNLGAVTLKNLISKYRGNDKAIAILLSRVDDFNRASQDTLPQLINSTEAEINNTLPQGRII
    KQSIPVVRLKVERLGSGAIKAESINNIKAESINKIQGKSTNTIKAESINKIKVESINTIKAESINKIQAK
    PINTIKAESINTIKAESIHKIKPQSIKSTSATHVKVSDQELAKQSRRSQDIIKSLGFLSSDQKDILVKSI
    SSSKGSQLILKFVTQATQLNNAESTKAKHMAQNDVASIKNISLEVLEEYKEKIQRASTKSQVDELVAEAK
    KVVNSNKETLVNQANGKKQEIAKLENLSNDEMLRYNTAIDNIVKQYNEGKLNITDAMNALNSIKQAAQEV
    AQKNLQKQYAKKIERISLKGLALSKKAKEIYEKHKSILPTPGYYA
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Santi et al., 2007)
8. BipA
  • Gene Name : BipA
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : 255961211
  • Other Database IDs : CDD:310807
    CDD:114603
    CDD:307064
  • Taxonomy ID : 1311
  • Gene Strand (Orientation) : ?
  • Protein Name : BibA
  • Protein pI : 9.74
  • Protein Weight : 59842.07
  • Protein Length : 648
  • Protein Note : MucBP domain; pfam06458
  • Protein Sequence : Show Sequence
    >ACU44481.1 BibA [Streptococcus agalactiae]
    MNNNEKKVKYFLRKTAYGLASMSAAFIVCSGIVNTPTVSADSPDTLKVEKLGKLKDVKSVHELTPISIPN
    ELKGAKEQALSSIISHPNITNSEVDKLASDYSFRINTSNDVNDVKRLLNEFYNAVARKQLDTNSADYRSK
    IDNISTTGLAIALEAKEIYEANKSILPHRYKDSVGTYVNSFEERRSPGKFNIWNGQEGFNAAQKLLEDVK
    KLLLELQNLTKNNKPNIQVPKQAPTEAAKPALSPEALTRLTTWYNQAKDLLKDDQVKDKYVDILSVQKAV
    DQAYDHVEEGKFITTDQANQLANKLRDALQSLELKDKKVAKPVAKGTYDVKYVDTEGKEVAKSRHFEGEE
    GAAFVTSAKEVAGYKLVRTEGAVSNVFTAGAQVRTYVYEKVAKPVAKGTYDVKYVDTEGKEVAKSRHFEG
    EEGAAFVTSAKEVAGYKLVRTEGAVSNVFTAGAQVRTYVYEKVKPEVKPDVKPEAKPEAKPEVKPDVKPE
    AKPEAKPEVKSDVKPEAKPEAKPEAKPEVKPDVKPEAKPEAKPATKKSVNTSGNLVAKKAIENKKYSKKL
    PSTGEAASPLLAIVSLIVMLSAGLITIVLKHKKN
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice immunized with BibA were protected against challenge by a GBS strain with high levels of surface exposure of the antigen (Santi et al., 2009).
  • Related Vaccine(s): S. agalactiae BipA Protein Vaccine
9. Branched-chain alpha-keto acid dehydrogenase subunit E2
  • Gene Name : Branched-chain alpha-keto acid dehydrogenase subunit E2
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : AFV72219
  • Other Database IDs : CDD:237001
    CDD:308457
  • Taxonomy ID : 1311
  • Protein Name : Branched-chain alpha-keto acid dehydrogenase subunit E2
  • Protein pI : 4.51
  • Protein Weight : 46030.05
  • Protein Length : 565
  • Protein Note : branched-chain alpha-keto acid dehydrogenase subunit E2; Reviewed
  • Protein Sequence : Show Sequence
    >AFV72219.1 Branched-chain alpha-keto acid dehydrogenase subunit E2 [Streptococcus agalactiae]
    MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKTNMEIEAEDSGVLLKITHGNGDVVPVT
    ETIGYIGAEGEEVTEASSSENTSVEENATQVTSEPEKVEETSEPSVPAATNGEKVRATPAARKLAREMSI
    DLALVSGTGANGRVHREDVESFKGAQPRITPLGRRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAA
    EAPVETKATPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLIDPIM
    AKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGLDDGLIVPVVHNADQMS
    LSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGE
    IVARPIMAMCLTIDHRIVDGMNGAKFMVDLKNLMEKPFGLLI
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Liu et al., 2013)
10. C protein alpha-antigen
  • Gene Name : C protein alpha-antigen
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : AIF88081
  • Other Database IDs : CDD:273479
    CDD:204077
    CDD:298076
    CDD:279132
  • Taxonomy ID : 1311
  • Protein Name : C protein alpha-antigen
  • Protein pI : 4.53
  • Protein Weight : 108142.91
  • Protein Length : 1263
  • Protein Note : Gram-positive signal peptide, YSIRK family; TIGR01168
  • Protein Sequence : Show Sequence
    >AIF88081.1 C protein alpha-antigen [Streptococcus agalactiae]
    MFRRSKNNSYDTSQTKQRFSIKKFKVGAASVLIGLSFLGGVTQGNLNIFEESIVAASTIPGSAATLNTSI
    TKNIQNGNAYIDLYDVKLGKIDPLQLIVLEQGFTAKYVFRQGTKYYGDVSQLQSTGRASLTYNIFGEDGL
    PHVKTDGQIDIVSVALTIYDSTTLRDKIEEVRTNANDPKWTEESRTEVLTGLDTIKTDIDNNPKTQTDID
    SKIVEVNELEKLLVLSVPDKDKYDPTGGETTVPQGTPVSDKEITDLVKIPDGSKGVPTVVGDRPDTNVPG
    DHKVTVDVTYPDGTKDTVEVTVHVTPKPVPDKDKYDPTGGETTVPQGTPVSDKEITDLVKIPDGSKGVPT
    VVGDRPDTNVPGDHKVTVEVTYPDGTKDTVEVTVHVTPKPVPDKDKYDPTGGETTVPQGTPVSDKEITDL
    VKIPDGSKGVPTVVGDRPDTNVPGDHKVTVEVTYPDGTKDTVEVTVHVTPKPVPDKDKYDPTGGETTVPQ
    GTPVSDKEITDLVKIPDGSKGVPTVVGDRPDTNVPGDHKVTVDVTYPDGTKDTVEVTVHVTPKPVPDKDK
    YDPTGGETTVPQGTPVSDKEITDLVKIPDGSKGVPTVVGDRPDTNVPGDHKVTVEVTYPDGTKDTVEVTV
    HVTPKPVPDKDKYDPTGGETTVPQGTPVSDKEITDLVKIPDGSKGVPTVVGDRPDTNVPGDHKVTVEVTY
    PDGTKDTVEVTVHVTPKPVPDKDKYDPTGGETTVPQGTPVSDKEITDLVKIPDGSKGVPTVVGDRPDTNV
    PGDHKVTVDVTYPDGTKDTVEVTVHVTPKPVPDKDKYDPTGGETTVPQGTPVSDKEITDLVKIPDGSKGV
    PTVVGDRPDTNVPGDHKVTVEVTYPDGTKDTVTVTIHVTPKPVPDKDKYDPTGGETTVPQGTPVSDKEIT
    DLVKIPDGSKGVPTVVGDRPDTNVPGDHKVTVEVTYPDGTKDTVEVTVHVTPKPVPDKDKYDPTGGETTV
    PQGTPVSDKEITDLVKIPDGSKGVPTVVGDRPDTNVPGDHKVTVEVTYPDGTKDTVTVTIHVTPKPVPDK
    DKYDPTGGETTVPQGTPVSDKEITDLVKIPDGSKGVPTVVGDRPDTNVPGDHKVTVEVTYPDGTKDTVTV
    TIHVTPKPDKDKYDPTGKAQQVNGKGNKLPATGENATPFFNVAALTIISSVGLLSVSKKKED
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Pannaraj et al., 2008)
11. eno
  • Gene Name : eno
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : AFV72009
  • Other Database IDs : CDD:234617
  • Taxonomy ID : 1311
  • Protein Name : Enolase
  • Protein pI : 4.34
  • Protein Weight : 44721.57
  • Protein Length : 490
  • Protein Note : enolase; Provisional; PRK00077
  • Protein Sequence : Show Sequence
    >AFV72009.1 Enolase [Streptococcus agalactiae]
    MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTGEHEAVELRDGDKSRYGGLGTQKAVDN
    VNNVIAEAIIGYDVRDQQAIDRAMIALDGTPNKGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTK
    VLPTPMMNIINGGSHSDAPIAFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAP
    KFEGTEDGVETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYSKFEGEGGAVRTAAEQIDYLE
    ELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIKEEAANSILIKVNQIGT
    LTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDQLGE
    VAQYKGIKSFYNFKK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Brzychczy-Wloch et al., 2013)
12. immunogenic secreted protein
  • Gene Name : immunogenic secreted protein
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : ASI67082
  • Other Database IDs : CDD:332523
  • Taxonomy ID : 1311
  • Protein Name : immunogenic secreted protein
  • Protein pI : 5.76
  • Protein Weight : 30076.11
  • Protein Length : 366
  • Protein Note : Surface antigen [Cell wall/membrane/envelope biogenesis]; cl27702
  • Protein Sequence : Show Sequence
    >ASI67082.1 immunogenic secreted protein [Streptococcus agalactiae]
    MSTGITYDSSRIDGQKILDWEKSSGLDARAIIAIAIAESSLGTQGVATAPGANMFGFGAFDNNTTNAQNF
    SDDKAVIKMTQETIIQNQNTSFAIQDQKAQLLSTGNLNVAARGGVYFTDASGSGKRRAAIMKSIDKWMID
    AHGGISEISKELLNTSSVAMMAVPTSYSVSRANQAGNYVAGTYPWGQCTWYVFNRTKELGYQFGPFMGNG
    GDWKNKPGYQTTHEAKTGYAISFSPGQAGADRTYGHVAIVEDVKEDGSILISESNVLGLGTISYRTFSAA
    EAAQLTYVVGEK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Li et al., 2016)
13. pcsB
  • Gene Name : pcsB
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : CAC28144
  • Other Database IDs : CDD:226400
    CDD:226451
    InterPro: IPR007921
    InterPro: IPR009148
    UniProtKB/TrEMBL: Q9AKA4
  • Taxonomy ID : 1311
  • Protein Name : PcsB protein
  • Protein pI : 8.94
  • Protein Weight : 42876.56
  • Protein Length : 507
  • Protein Note : Uncharacterized N-terminal domain of peptidoglycan hydrolase CwlO [Function unknown]; COG3883
  • Protein Sequence : Show Sequence
    >CAC28144.1 PcsB protein [Streptococcus agalactiae]
    MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSGQQAAAQNQVTAIKGQVGALESQQSEL
    EAQNAQLEAVSQQLGQEIQTLSNKIVARNESLKKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREV
    VSANEKMLAQQEADKAALEAKQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNE
    KASLIQAKAQAEEAARKAAEAQAAAEAKAQAEAKAQAESVAKAQAAAQVESATAPTETVQTQPRTEIKPS
    NLTATSSATTVATTTATATNEPKVTQPSVVTKAVEAPKAVVSSTPRAVSKPVVRSYDSSNTYPMGQCTWG
    AKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWPYDGGGYGHVAVVTSVANNSSIQVMESNY
    AGNMSIGNYRGSFNPSASGSVYYIYPN
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Li et al., 2016)
14. peptidase M15
  • Gene Name : peptidase M15
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : ASI65231
  • Other Database IDs : CDD:224600
  • Taxonomy ID : 1311
  • Protein Name : peptidase M15
  • Protein pI : 9.93
  • Protein Weight : 43880.1
  • Protein Length : 471
  • Protein Note : D-alanyl-D-alanine carboxypeptidase [Cell wall/membrane/envelope biogenesis]; COG1686
  • Protein Sequence : Show Sequence
    >ASI65231.1 peptidase M15 [Streptococcus agalactiae]
    MKKIITSILLLSCIFFMPTISAESFNASAKHALAVDLDSGKILYEKDANKPAAIASLTKIMTVYMVYKEI
    DNGNLKWNTKVNISDYPYQLTRESDASNVPLEKRRYTVKQLVDAAMISSANSAAIALAEHISGTESKFVD
    KMTAQLEKWGIHDSHLVNASGLNNSMLGNHIYPKSSQNDENKMSARDIAIVAYHLVNEYPSILKITSKSV
    AKFDKDIMHSYNYMLPDMPVFRPGITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKYARFT
    ATNSLLNYITNTYEPNLVLAKGASYKGKEASVRDGKEQSVIAVAKNDLKVAQKKNITKQNQLKINFKKEL
    TAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIERSFFLKVWWNHFVRYVNEKL
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Li et al., 2016)
15. rib
  • Gene Name : rib
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae strain BM110
  • VO ID : VO_0012403
  • NCBI Protein GI : 1620648
  • Other Database IDs : CDD:309682
    CDD:312387
    CDD:323788
    CDD:307064
  • Taxonomy ID : 1311
  • Gene Strand (Orientation) : ?
  • Protein Name : surface protein Rib
  • Protein pI : 4.28
  • Protein Weight : 113797.43
  • Protein Length : 1309
  • Protein Note : YSIRK type signal peptide; pfam04650
  • Protein Sequence : Show Sequence
    >AAC44468.1 surface protein Rib [Streptococcus agalactiae]
    MFRRSKNNSYDTLQTKQRFSIKKFKFGAASVLIGISFLGGFTQGQFNISTDTVFAAEVISGSAVTLNTNM
    TKNVQNGRAYIDLYDVKNGKIDPLQLITLNSPDLKAQYVIRQGGNYFTQPSELTTVGAASINYTVLKTDG
    SPHTKPDGQVDIINVSLTIYNSSALRDKIDEVKKKAEDPKWDEGSRDKVLISLDDIKTDIDNNPKTQSDI
    ANKITEVTNLEKILVPRIPDADKNDPAGKDQQVNVGETPKAEDSIGNLPDLPKGTTVAFETPVDTATPGD
    KPAKVVVTYPDGSKDTVDVTVKVVDPRTDADKNDPAGKDQQVNVGETPKAEDSIGNLPDLPKGTTVAFET
    PVDTATPGDKPAKVVVTYPDGSKDTVDVTVKVVDPRTDADKNDPAGKDQQVNVGETPKAEDSIGNLPDLP
    KGTTVAFETPVDTATPGDKPAKVVVTYPDGSKDTVDVTVKVVDPRTDADKNDPAGKDQQVNVGETPKAED
    SIGNLPDLPKGTTVAFETPVDTATPGDKPAKVVVTYPDGSKDTVDVTVKVVDPRTDADKNDPAGKDQQVN
    VGETPKAEDSIGNLPDLPKGTTVAFETPVDTATPGDKPAKVVVTYPDGSKDTVDVTVKVVDPRTDADKND
    PAGKDQQVNVGETPKAEDSIGNLPDLPKGTTVAFETPVDTATPGDKPAKVVVTYPDGSKDTVDVTVKVVD
    PRTDADKNDPAGKDQQVNVGETPKAEDSIGNLPDLPKGTTVAFETPVDTATPGDKPAKVVVTYPDGSKDT
    VDVTVKVVDPRTDADKNDPAGKDQQVNVGETPKAEDSIGNLPDLPKGTTVAFETPVDTATPGDKPAKVVV
    TYPDGSKDTVDVTVKVVDPRTDADKNDPAGKDQQVNVGETPKAEDSIGNLPDLPKGTTVAFETPVDTATP
    GDKPAKVVVTYPDGSKDTVDVTVKVVDPRTDADKNDPAGKDQQVNVGETPKAEDSIGNLPDLPKGTTVAF
    ETPVDTATPGDKPAKVVVTYPDGSKDTVDVTVKVVDPRTDADKNDPAGKDQQVNVGETPKAEDSIGNLPD
    LPKGTTVAFETPVDTATPGDKPAKVVVTYPDGSKDTVDVTVKVVDPRTDADKNDPAGKDQQVNVGETPKA
    EDSIGNLPDLPKGTTVAFETPVDTATPGDKPAKVVVTYPDGSKDTVDVTVKVVDPRTDADKNDPAGKDQQ
    VNGKGNKLPATGENATPFFNVVALTIMSSVGLLSVSKKKED
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Vaccination of mice with the Rib protein protected against two strains of capsular type III and two strains of type II, and vaccination with the alpha protein protected against one strain of type II and one strain of type Ib (Larsson et al., 1996).
  • Related Vaccine(s): S. agalactiae Rib Protein Vaccine
16. sar5
  • Gene Name : sar5
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae Compton R
  • VO ID : VO_0012393
  • NCBI Protein GI : 5327234
  • Other Database IDs : CDD:273479
    CDD:283989
    CDD:273478
    GOA:Q9XAS7
    InterPro: IPR001899
    InterPro: IPR005877
    UniProtKB/TrEMBL: Q9XAS7
  • Taxonomy ID : 1311
  • Gene Strand (Orientation) : ?
  • Protein Name : BPS protein
  • Protein pI : 4.92
  • Protein Weight : 96916.55
  • Protein Length : 1045
  • Protein Note : Gram-positive signal peptide, YSIRK family; TIGR01168
  • Protein Sequence : Show Sequence
    >CAB46338.1 BPS protein [Streptococcus agalactiae]
    MFRQYNFEKGLNFSIRKFSVGIASVAIGSLLFILPQVLADETTSVTSATTPTGVTTTDANLVNPNNSTPT
    STNRSATSTQGSNLSNTSEIIKPATLAATSPTTDNVAPSVDKRTYATSGDWTLQNPYADSVRNKNISPSV
    RHESFKSAETTVVRHDNSTVKVTATITPVEGNDEGSGILTNGGNQSEYKATSEMFVGNVDPAKIPALGVY
    TQPGRTEGGSKLSDKLNFNGKAPSTILTLKFDKAVTDPIIDLSGVGGNARLSFTETVMENGKIVEKFDYA
    RGSYNSTFFDGITPGISLEKASSGVNLTVTANTVDVTDKNTFNESVVNPSDEDFVNGPDRTPDAVPAGTG
    SIRLKGTFTDASFKLYHQAVPSTAFSKEKYHTGDGYYNSIANVRPTVVKPDSINGLNKHASDVIDYKISD
    NNDISNDDLLRLSVRLQNPRGSVVVNYIDTEGNIIGTEYKDTTDAIPGTHYNTAESSGDLNSDATVERPS
    TITKDGKVYDLVAENITVPVGKVNSDGTLATNGSSFNYGTDAASAEVAEGTKSVIYVISIKQESKGNVHA
    RYVILGTETELASAKTVKSEAPIDEAYSDKAPATLEKDGKLYEFVHVRDNKGDAPADGKVTEQDQTITYE
    YVEVPKGRVVVDYVIEGTATKPKDTYVDTPTAYIRDKEGQAIPYNTAENDSEKPLLLDKDGIKYELVSIQ
    EGSAAEKGTLREGEQHVVYQYRKVVEVPSVKVGNVHARYVILGTETELASAKTVKSEAPIDEAYSDKAPA
    TLEKDGKLYEFVHVRDNKGDAPADGKVTEQDQTITYEYKLKKDDADAVGNVVINYVDETGNVIKKPILDT
    HESKVGTPYDTTDYKFAEIKFNGKIYKLVSAKTMGNEFGKVTEGTTEVTYVYRESVKSTLPKETGISIPS
    ESESPKFISTQTTENRPNKGVLTSSKNPTNKSVLPTTGEESNRILGVVGITLVATTATLAASSLKRRKN
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Immunization of mice with recombinant BPS protein by the subcutaneous route produced an efficient antigen-specific response, and immunized animals survived challenge with a lethal dose of a virulent strain (Erdogan et al., 2002).
  • Related Vaccine(s): S. agalactiae BPS Protein Vaccine
17. scpA_4
  • Gene Name : scpA_4
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : AMQ15511
  • Other Database IDs : CDD:282500
    CDD:173801
    CDD:278510
    CDD:239048
    CDD:283851
  • Taxonomy ID : 1311
  • Protein Name : C5a peptidase precursor
  • Protein pI : 8.96
  • Protein Weight : 72430.19
  • Protein Length : 796
  • Protein Note : predicted cleavage at residue 36
  • Protein Sequence : Show Sequence
    >AMQ15511.1 C5a peptidase precursor [Streptococcus agalactiae]
    MNTKQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSDIAVEQQVKDSPTSIANETPTNNTSSA
    LASTAQDNLVTKANNNSPTETQPVAESHSQATETFSPAANQPVESTQEVSKTPLTKQNLAVKPTPAISKE
    TPQNIDSNKIITVPKVWNTGYKGEGTVVAIIDSGLDTNHDALQLNDSTKAKYQNEQQMNAAKAKAGINYG
    KWYNNKVIFGHNYVDVNTELKEVKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSDEKRG
    TGPALYVKAIEDAVKLGADSINLSLGGANGSLVNADDRLIKALEMARLAGVSVVIAAGNDGTFGSGASKP
    SALYPDYGLIGSPSTAREAISVASYNNTTLVNKVFNIIGLENNKNLNNGLAAYADPKVSDKTFEVGKQYD
    YVFVGKGNDNDYKDKTLNGKIALIERGDITFTKKVVNAINHGAVGAIIFNNKAGEANLTMSLDPEASTIP
    AIFTQKEFGDVLAQKSYKIVFNNIKNKQANPNAGVLSDFSSWGLTADGQLKPDLSAPGGSIYAAINDNEY
    DMMSGTSMASPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTGAYTSPRQQGAGII
    DVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTYLNTDQVKDGFVTLAPQQL
    VHLQGKRYGLNQGKLKRLQLI
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Santillan et al., 2011)
18. scpB
  • Gene Name : scpB
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : AAB17762
  • Other Database IDs : CDD:173801
    CDD:278510
    CDD:239048
    CDD:283851
    CDD:304423
  • Taxonomy ID : 1311
  • Protein Name : SCPB
  • Protein pI : 5.96
  • Protein Weight : 120176.88
  • Protein Length : 1212
  • Protein Note : human group B streptococci
  • Protein Sequence : Show Sequence
    >AAB17762.1 SCPB [Streptococcus agalactiae]
    MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQTVETPQPTAVSEEAPSSKETKTPQTPS
    DAGETVADDANDLAPQAPAKTADTPATSKATIRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAW
    RLTDKTKARYQSKEDLEKAKKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETK
    EPYRLEGAMPEAQLLLMRVEIVNGLADYARNYAQAIRDAINLGAKVINMSFGNAALAYANLPDETKKAFD
    YAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSPDKQLTETVRVKTADQQD
    KEMPVLSTNRFEPNKAYDYAYANRGTKEDDFKDVKGKIALIERGDIDFKDKIAKAKKAGAVGVLIYDNQD
    KGFPIELPNVDQMPAAFISRKDGLLLKDNPQKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIA
    APGQDILSSVANNKYAKLSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDED
    EKAYFSPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVNVHNKSDKPQELYYQATVQT
    DKVDGKHFALAPKVLYEASWQKITIPANSSKQVTVPIDASRFSKDLLAQMKNGYFLEGFVRFKQDPKKEE
    LMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDAKDQLDGDGLQFYALKNNFTALTTESNPWTI
    IKAVKEGVENIEDIESSEITETILAGTFAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLR
    NAKNLVAEVLDKEGNVVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYT
    PISSGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDEDLPTTEYIS
    PNEDGTFTLPEEAETTEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEGHSNKPEQDGSDQAPDKKPEA
    KPEQDGSGQTPDKKTETKPEKDSSGQTPGKTPQKGQPSRTLEKRSSKRALATKASTRDQLPTTNDKDTNR
    LHLLKLVMTTFFLGLVAHIFKTKRQKETKK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Suvorov et al., 2010)
19. Sip
  • Gene Name : Sip
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae A909
  • VO ID : VO_0012402
  • NCBI Gene ID : 3685788
  • NCBI Protein GI : 76788047
  • Locus Tag : SAK_0065
  • Genbank Accession : CP000114
  • Protein Accession : YP_328758
  • Other Database IDs : CDD:212030
    CDD:331378
  • Taxonomy ID : 205921
  • Gene Starting Position : 47330
  • Gene Ending Position : 48634
  • Gene Strand (Orientation) : +
  • Protein Name : group B streptococcal surface immunogenic protein
  • Protein pI : 8.59
  • Protein Weight : 40547.64
  • Protein Length : 434
  • Protein Note : Lysine Motif is a small domain involved in binding peptidoglycan; cd00118
  • DNA Sequence : Show Sequence
    >NC_007432.1:47330-48634 Streptococcus agalactiae A909, complete genome
    AATGAAAATGAATAAAAAGGTACTATTGACATCGACAATGGCAGCTTCGCTATTATCAGTCGCAAGTGTT
    CAAGCACAAGAAACAGATACGACGTGGACAGCACGTACTGTTTCAGAGGTAAAGGCTGATTTGGTAAAGC
    AAGACAATAAATCATCATATACTGTGAAATATGGTGATACACTAAGCGTTATTTCAGAAGCAATGTCAAT
    TGATATGAATGTCTTAGCAAAAATTAATAACATTGCAGATATCAATCTTATTTATCCTGAGACAACACTG
    ACAGTAACTTACGATCAGAAGAGTCATACTGCTACTTCAATGAAAATAGAAACACCAGCAACAAATGCTG
    CTGGTCAAACAACAGCTACTGTCGATTTGAAAACCAATCAAGTTTCTGTTGCAGACCAAAAAGTTTCTCT
    CAATACAATTTCGGAAGGTATGACACCAGAAGCAGCAACAACGATTGTTTCGCCAATGAAGACATATTCT
    TCTGCGCCAGCTTTGAAATCAAAAGAAGTATTAGCACAAGGGCAAGCTGTTAGTCAAGCAGCAGCTAATG
    AACAGGTATCACCAGCTCCTGTGAAGTCGATTACTTCAGAAGTTCCAGCAGCTAAAGAGGAAGTTAAACC
    AACTCAGACGTCAGTCAGTCAGTCAACAACAGTATCACCAGCTTCTGTTGCCGCTGAAACACCAGCTCCA
    GTAGCTAAAGTAGCACCGGTAAGAACTGTAGCAGCCCCTAGAGTGGCAAGTGTTAAAGTAGTCACTCCTA
    AAGTAGAAACTGGTGCATCACCAGAGCATGTATCAGCTCCAGCAGTTCCTGTGACTACGACTTCAACAGC
    TACAGACAGTAAGTTACAAGCGACTGAAGTTAAGAGCGTTCCGGTAGCACAAAAAGCTCCAACAGCAACA
    CCGGTAGCACAACCAGCTTCAACAACAAATGCAGTAGCTGCACATCCTGAAAATGCAAGGCTCCAACCTC
    ATGTTGCAGCTTATAAAGAAAAAGTAGCGTCAACTTATGGAGTTAATGAATTCAGTACATACCGTGCGGG
    AGATCCAGGTGATCATGGTAAAGGTTTAGCAGTTGACTTTATTGTAGGTAAAAACCAAGCACTTGGTAAT
    GAAGTTGCACAGTACTCTACACAAAATATGGCAGCAAATAACATTTCATATGTTATCTGGCAACAAAAGT
    TTTACTCAAATACAAATAGTATTTATGGACCTGCTAATACTTGGAATGCAATGCCAGATCGTGGTGGCGT
    TACTGCCAACCACTATGACCACGTTCACGTATCATTTAACAAATA
    
    
  • Protein Sequence : Show Sequence
    >YP_328758.1 group B streptococcal surface immunogenic protein [Streptococcus agalactiae A909]
    MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADLVKQDNKSSYTVKYGDTLSVISEAMSI
    DMNVLAKINNIADINLIYPETTLTVTYDQKSHTATSMKIETPATNAAGQTTATVDLKTNQVSVADQKVSL
    NTISEGMTPEAATTIVSPMKTYSSAPALKSKEVLAQGQAVSQAAANEQVSPAPVKSITSEVPAAKEEVKP
    TQTSVSQSTTVSPASVAAETPAPVAKVAPVRTVAAPRVASVKVVTPKVETGASPEHVSAPAVPVTTTSTA
    TDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENARLQPHVAAYKEKVASTYGVNEFSTYRAG
    DPGDHGKGLAVDFIVGKNQALGNEVAQYSTQNMAANNISYVIWQQKFYSNTNSIYGPANTWNAMPDRGGV
    TANHYDHVHVSFNK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Immunization with the recombinant Sip protein efficiently protected CD-1 mice against deadly challenges with six GBS strains of serotypes Ia/c, Ib, II/R, III, V, and VI (Brodeur et al., 2000).
  • Related Vaccine(s): S. agalactiae Sip Protein Vaccine
20. srr-1
  • Gene Name : srr-1
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : AKI95824
  • Other Database IDs : CDD:274741
    CDD:275065
    CDD:287406
    CDD:273478
  • Taxonomy ID : 1311
  • Protein Name : Serine rich repeat protein 1
  • Protein pI : 5.03
  • Protein Weight : 103871.63
  • Protein Length : 1163
  • Protein Note : KxYKxGKxW signal peptide; TIGR03715
  • Protein Sequence : Show Sequence
    >AKI95824.1 Serine rich repeat protein 1 [Streptococcus agalactiae]
    MSKKTFGKQLTVVGSKSRVKMHKSGKNWVRTIMSHFNLFKAIKGRATVEADVCVQNIEKEDRLSSGNLTY
    LKGLLAAGALLGGASLTSRVYADETPVVQEQSSSVPTLAEQTEVTVKTTAVQNYQDGTVSKNIIDSNSVS
    MSESASISVSESISMSMSGSISTSVSESVSTSTSESTLSSLSESASTSALKSTSVSEASNILETQASLTD
    KGRESFSANQIVTESSLVTDAGKNASVSSLIEITKPKSELQTSKMSNESLITPEKPQVMIASDKTGNETL
    TPTIRLKSVIQPRSMNLIALSSEMDLIPLEEVSDTEMLGKDVSSEFQKVNIALKDNTLSEPGTVKLDKSE
    NLVLNFDFSIASVNEGDFFTVKLSDNLDTQGVGTTLKVQDIIDTSGQLLATGSYSPLTHNITYIWTKYAS
    TLNNINAQVKLPVWPDQRKVSKTTSDKQRFTATLNNQVASIEERVQYNSPSVTEHTNVKTNVRSRIMKID
    DEKQTETYITQINPEGKEMYFASGLGNLYTIIGSDGTSGSPVNLLNAEVKILKTNSKNLTDSMDQNYDST
    EFEDVTSQYSYTNDGSKITIDWKTNSISSSTSYVVLVKIPKQSGVLYSTVSDINQTNGSKYFYGYTQVDG
    ESDANAEKNFLSESASTSASTSASTSASASASTSASISASTSASISASTSASTSASTSASTSASMSTSTS
    VSTSASTSASMSASTSASTSASTSASTSASTSASTSASTSASTSASTSASTSASMSASMSASTSVSTSAS
    TSASTSASTSASTSASMSASMSASTSASMSASTSASTSASMSASTSASTSASTSASTSASTSASMSASTS
    ASTSASMSASTSASTSASTSASTSASTSTSTSASTSASMSASTSASTSASTSASMSASTSASISASTSAS
    MSASTSASTSASTSASTSASMSASTSASTSASTSASTSASMSASTSASTSASMSASTSASTSASTSASMS
    ASTSASTSASMSASTSASMSATTSASTSVSTSASTSASTSASTSSSSSVTSNSSKEKVYSALPSTGDQDY
    SVTATALGLGLMTGATLLGRKKSKKDKD
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Liu et al., 2013)
21. tig
  • Gene Name : tig
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : AFV71595
  • Other Database IDs : CDD:234956
  • Taxonomy ID : 1311
  • Protein Name : Trigger factor
  • Protein pI : 4.14
  • Protein Weight : 43078.51
  • Protein Length : 489
  • Protein Note : trigger factor; Provisional; PRK01490
  • Protein Sequence : Show Sequence
    >AFV71595.1 Trigger factor [Streptococcus agalactiae]
    MSTSFENKATNRGIITFTISQDEIKPALDQAFNKVKKDLNVPGFRKGHMPRTVFNQKFGEEALYENALNL
    VLPKAYEAAVAELGLDVVVQPKIDVVSMEKGQDWKLTAEVVTKPEVKLGDYKDLSVEVDASKEVSDEEVD
    AKVERERNNLAELTVKDGEAAQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAG
    QTVDVNVTFPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDDEVETLDVLKAKYRKELESAK
    EIAFDDAVEGAAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEMYFQLTGTTEEDLHKQY
    QADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYNMEADQVRGLLSADMLKHDIAMKKAVDVI
    TSSATVK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Brzychczy-Wloch et al., 2013)
22. tuf
  • Gene Name : tuf
  • Sequence Strain (Species/Organism) : Streptococcus agalactiae
  • NCBI Protein GI : KUH47811
  • Taxonomy ID : 1311
  • Protein Name : elongation factor Tu
  • Protein pI : 4.49
  • Protein Weight : 38501.39
  • Protein Length : 454
  • Protein Note : EF-Tu; promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis; when the tRNA anticodon matches the mRNA codon, GTP hydrolysis results; the inactive EF-Tu-GDP leaves the ribosome and release of GDP is promoted by elongation factor Ts; many prokaryotes have two copies of the gene encoding EF-Tu; Derived by automated computational analysis using gene prediction method: Protein Homology.
  • Protein Sequence : Show Sequence
    >KUH47811.1 elongation factor Tu, partial [Streptococcus agalactiae]
    VDHGKTTLTAAITTVLARRLPTSVNQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHAD
    YVKNMITGAAQMDGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
    LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPVEDVFSITGRGTV
    ASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAGDNVGVLLRGVQRDEIERGQVLAKP
    GSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQFYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHP
    IAVEQGTTFSIREGGRTVGSGIVSEIEA
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Brzychczy-Wloch et al., 2013)
1. IgG
  • Gene Name : IgG
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16059
  • Genbank Accession : AF010213
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 881411
  • Gene Ending Position : 914690
  • Gene Strand (Orientation) : +
  • Protein Name : immunoglobulin heavy chain (V7183 family)
  • Protein Note : Also known as IgG; IgH; VI24H; VH7183; B9-scFv; IgVH1(VSG)
  • DNA Sequence : Show Sequence
    >gi|94393741:881411-914690 Mus musculus strain 129/SvJ chromosome 12 unlocalized genomic contig, MGSCv37 alternate locus group 129/SvJ
    CATGGACTTTGGGCTCAGCTGTGTTTTCCTTGTCCTCATTTTAAGAGGTAATTTGTAGAAATAAGATCCT
    GCCAGTATTGTGTACAGGAGAAATAGAAAAATTTTTCTTTCCTCTATTTTGTTTTGTTTTGTTAGTGACA
    GTTTACAAATAAGCATTCTCTGTTGTGAGGTGCCCAGTGTGAGGTGAAGATGGTGGAGTCTGTGGGAGGC
    TTAGTGCAGCCTGTAGGGTCATTGAAACCCTCCTGTGCAGCCTCTGGATTCATTCTCACTGACTACTGAA
    TGACCTGGATCCTTCAGGCTTCAAAGAAAAGGATGGAGAGGGTGACAATAAAATTTTTCCCACTCACACC
    CACTTGCTGGCCCTGGCGTTCCCCTGTAATGAGGCACATAAAGTTTGCAAAACCAAGGGGCCTCTCTTCT
    CAATGATGGCTGACTAGGACATCTTCTGATACATATGCAGCTAGAGACATGAGCTCCCGGGATACTGGTT
    ACTTCATATTGTTGTTCCCCCACAACAGTTGCAGACCCTTTTATTTCTGTGGGTACTTTCACTAGCTCCT
    CCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTAGTTGCTAGGCCCCGG
    CATAGCCTCACAAGAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTTGCTGGTGTATGCAATGGTGTC
    AGAGTTTGGAGTCTGATTATGGGATGGATCCCCAGGTATGGCAAACTCTAGATGGTCCATCCTTTCCCAA
    CAATAATTTTTAATGGTGGAGATAGCACCTACTATCCAGACACAATGAAGGGCCAATTCACTATCTCTAG
    AGATGATGCCAAAAACACACTTTACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTATACA
    TATATTCCTAAGCTTGAGTAAGTGGACTTAAACTGATTCCATCACAACTTGCATGAGATGGATATTCCCC
    AGTGTTAAGACCTGTCACCATCACTGTCAATCAGAAGACAAAGTTTATGCACAACAAAACAAAAAAACCA
    AAAGCAGAGGCCTCCAATTACAAGTAATAGACCCAGACCCACAGTCTCTGAAAACTGACTGTACAGTTGG
    ATCCAGTCTTTTACTTCTTTTCCCTGGATTTTATATTACTGAGGAAATGAGGAAAGCTCTACAATATCTG
    TTCTCCATAGTGCTCAACACCTCCAAGCACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTTCTT
    GTCTTGTAGACTACTTCAACCTATTTTGTACTCCAGTTAATGAAACTCAAGTCTAGCAGCCTGTCACTGT
    TTATTCTAAAGTATTATGAACAGGTGACCTCCCATCCTTCCCCAACGCAATAATCATATTTAGGAATTTG
    AGGTTTTATGAGATATGATCTCAGGGTAGAGAGAGAAAGCAAACTACATAGAAATATAGACTGACATAAA
    TCAAGACTTGCATAAGCTAGTCCCCAAGTTCCATGTCCCTAAGTGGCAAGGACTATCTTCTGAGCCTAAT
    GAGATGAGATCCAAATCAAACCTCCTGGGTCTTTTTAGATAAACATGTGAGATCAATAGACTAAATGCTT
    TGGCTGGGCTTCCTTGCAATCCAATTCCCAAACAAAAATGGATCTGGCTCCACAGACACCACAAGAATAG
    TCTTAAATAGTTCTTTAAGTAGAATGTCTGATCACTACGAGCCCAATTCCATCCTAAATACTCTTCTGGA
    TTATACATAAATAAAAATTGAACATAGGGCATGGGGCACTGATCTCCCTGTGCTACATGAATGGGGGCTC
    ATTTACTAAATGTTCCCATTTTTCTTTCTAGCGCTGCACAGTGCAAATCCTACAACTTCCTGTTTATCTA
    CAATGTGAACTCCAAACAGTACAAGAAAAAACGTTCCTTATGTTCCTCTCCAGGTTCTCCAACAAGCACA
    GAGCAACCTTCTGTCACCAGGACGGAAAACTGGAAACCTTGCTCACTGCTTCCTTTTATTCCCTCGGGAA
    CCTCCCCCAATGCAAAGCAGCCCTCAGGCAGAGGATAAAAGCTCACACAAAGACGAGAAGCCCCATCCTC
    TTCTCATAGAGCATCCATCAGAGCATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACTTTCCCAAGCT
    GTAAGTGTTTCAGGGTTTCAGAAGAGGGACTAAGACATGTCAGCTAGAAGATGTGTGACTAATGGTGATG
    TTGCTTGTCCCCAGGTGTCCTGTCCCAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCA
    CAGAGCCTGTCCATCACATGCACCGTCTCAGGGTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCC
    AGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGC
    TCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTC
    CAAACTGATGACACAGCCATGTACTACTGTGCCAGAAACACAGTGTGGGAAGTCCAATGTGAGCCTGCAC
    AAATACTTCTCTGCAGGGATGATCACAACCAGCAGGGGGCGCTGATGACCCAAAGGGACTTCCCAGGATC
    TCTTCTGGAATCTAGGGAGTTCTGGCCTGTGTCTATCAGCATGTGTTTCAATGTTAGAGTTGTGAGTTTT
    CCTTCCAGCAACAGAGATATTTTAGAGCCCACTTTTCATGGTCATTCTACTAAATTTGTTCACATAGTGG
    AAAGATTTGTTAAATGATTCTATAGTCTAATAGGGTCAAACAAAAACAAATAATTGAGTTCATATCTACC
    AAAAAAAAAAAAATTCCCTCAAAGTGGACAACTGTGTAGGTGAGGAAAACCAGGGGGATTTGGGAATAGA
    TTATTTCTCTCCCGACTGTGGTGTTAGTCACCTTTCAGCCATTTACTGTATTTAATTCATGTTTAAAGTT
    AATTGTATTCATCTTCCCTAACATGAAGTTTTCAAACACATGTCCACAATTTAATGATGACACAGTTATC
    ATTGTGAATGATAACTCATAAGATCTCTCTCCATATATGAAACACACGATATTCTGTTATTAGCTATAGC
    CAACAAAGTTACATATCATTATTATTACTGAAATAATTCTTCCCATCTAACTGAATAGTTTTCTCACTCG
    ACACTGCCTATGCTGCGATCAGCCTGCTCTAATTTTACCTTTGCTCAGAGCACTGCTTTCTGTTTCATTT
    GATAAGATGGTGCCTGGTTGTCACTGAAAATGTGTCCTCCAGTTTCACAGTGATTAATGTGATTTTCAGT
    ACATTGAGAACAGAGCCACAGCAAAAATGAGATGGAATGTCATCATTTTTACGCCAGGTGCTGCAGAAAT
    AGCTAGGTGGTTAAGAGAGAATATTGAACTTTGAGGGGTTCAGATTAAATTCCTGATACCCACAATGTGC
    ACCTCATAACCACATGTACATATAGAAAACTCAGTATAAATGTGGCCTCCGTGAGCACCACACTACTCCC
    ACAAACACATATACACATAATTAAAAGTAAAGATTTTAGAAAAATGGCTAATCTAATGATAGGAAGTAGT
    CAAAAAGAGAGTTCTTTTGTCATGTACATATGTGTAGCAGACTTAAATGTTAAACATTCAAGAATACATT
    CCTCGATCCACATTAAAATTTTGCAAAGAGTAGCACAGACGGTGGCAATTGCTAAACTTATATCTAGAAA
    CACAATTATGTGTGGTGATATTTAATTACACATTTATACCAGGACATATGACAATATGGAAACCAAACAT
    GTTGTATCCACATGCTCTAAGGAAAACTAAATGGAGTGTGATAAAACCAAAGAAAATGTGAATATGAAAT
    ATTTTTCCAACTCTGCATCTTAAAACGGTTTCTTTCATGTGTCATATCTGCTATGAGGACTTTCTTCTGC
    CCATGTCCAACTCCAGAGCATGCCACAGCAGGAAGACCTACAGGTATTACTTCTCTGCACCCAGGAAAAC
    CACCTCTGTCCTGACCCTGCAGCTCTCAGAAGAGCCCAGACCTTCATTCTCAGGCCCTCATCCAGTAATC
    AGCACTGAATACAGAGCACTCACCATGGACTTTGGGCTCAGCTTGGTTTTCCTTGTCCTTATTTTAAAAG
    GTAATTCATAGAGATAAGATTCTATCTGTTTTGTGTACATGAGAAACAGAAAAATTGTATTGTTTCTCTA
    TTTTGTTTTGTTTTGTTAGTGACAGTTTCTGACTCAAGATTCTCTGTTTGAAGGTACCCAGTGTGAGGTG
    AAGCTGGTGAAGTCTAAGGGGAGGCATAGTGCAGCCTAGAAGGTCCATGATACTCTACTGTGCAGCCTCG
    GATTCACTGTAAGTGACGACTGGTTTGTCCGTGTTTGCCAGGCTCCAAAGAAGGGGCTGCAGTGGGGGAT
    GGGAATAATTTTTCATGGTTGTGGTAGCCCCTCTTATGCAGACACCTTGAAGAAGTGGGTTGGACGTAAC
    ATATTCAGAATCAATATTTAAAGATTCTAATCCTTGAAGATATCACTTTTGACCAAGTATATATGAACCA
    TGTTACTGAGGTTTATGGAGGTTTGAGTATGTTAGGTCCATGGATAGGGAAAATATTAGGGGATTTTAGG
    AGTAACTGAGGCTTGTTGTAGGAAGGACATCACTGTAGGGGTAGGCTCCGTGTTCCTATCTTCAAGCTCT
    ACCCAGTGCAGAATAGAGCCCTCTTCTGCCGGCAAGTGGAAAGAGTTTCTCTTCCTGGCTGCCTTCAATC
    CAAGTTGTAGAATATCAAATCTCCTAACACTATGACTGCGAGCATGCTGACATGCGTCCCACCATAAAAA
    TAGACTGAACCTCTGAAGCTTTAAGCCAGCCTCTAGTAACTGTATTCTTTAATAAGACTGAACTTGGCTA
    TGGTGTCTTTTCACAGTAAAATGGAAACATAGACAAGGTTCTAACTCTCTGTACTAGAGGACCATCTCTA
    TGTCCTTGGTGTTGTATGTAAATTATTGAACAAATACTATAGACAAGTTGTATGGCCAAAAGTCCATCCC
    CTTCAACAATCTACTATCTGGAACCATATAGATTAGATTCCTTTCCTGCACTCATTTCCCTTACTTGCTG
    AGACATTTTGAAGACATGCTCAGAATCCCTGAACTTCCTGCTGAAAAAAAAATACCCCTCCAATTTGAAG
    ACAGTTCTCTTCCAAAATTCATCATAAATACTGATCCACATGTCCAGGCATATCATGTAGTTTTAAAAAA
    CAAACCAATCAATGCTAAAGTGGGTAAGTGCCTACATCATTGGTTTATGTCTGTTCATAACCTGGTATAT
    AGCTAGAGACCAGGACTGCATGTTTCTCCTAGGCCACACCTCTCCCACAAATGCTGGCTCTGCCTCTCCA
    GAATTCCAAGATCATGGATCTCTGGGTCCCCAAGGAACAATAAGTGTGCACTCTTGCCAGCCTGTACACT
    ACTGCACTAGCTTCTCCCCCTGGAGCTTGGTCATTGCTCAATCTTCCCTTTCCAAAGCCTGGCCAAATTC
    CTCCTACCTTTTGTTCTTTCAGTCAGGCCACCTAAGTCATCTTGAATGAAAAACAACACAAAATCTGAAC
    TTAAAATCAACAAATAATACAAGTGCTGCGCACAAAATCAATCATTCTAAACTCATCAACTATTTTATGA
    TGGAAATCTTCCAACATACAAGCTACCACCAGGTCTAAGAATTACTCATCTACATGTTGCTTCCTCTCAC
    TCACAGCCTAACCATAAAAGGGCTGTTTCTTCCTCCCATGTCCCCTCTTTTGCCTTCAGAAGCAGAAGCT
    CCACCTCCTCCTCTTTGCCCAGCAATTGGCTTCTTGTCGTCTTTATTATCATATTAATTACTTAGGGGAA
    AATCCCGTGTAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGG
    AAGGCTCACCAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAG
    CCATAGGGGCTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACG
    CCTTTCATCTGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAA
    TATAAGGACATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTG
    AGTAACTGCTAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGA
    CTCTAAGTCATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTG
    ACTAATACAGAAGTTGGTACCAGGAGTGGTTCTAGAGTAACAGAAGTACAAGGATGAATCTTTTAAAATA
    CTGGAATTGGCTTGTTGATCCACCAGCACTTTCAACTATTGAAACCTCTCCAGATTCTCTCCCTCCTGGG
    AGCTCAGAGAATTTTGAAGACCCATGGTTGAAACTATATTCCGAACTTAAAGAAGCAAATGCCCTTGATT
    TTCTTAATGAATTAGGTGATTCAGTGCACAAAGCTTTCTACAAGATGGGGAAAAAATTGGAAAATGATTT
    TACTGGCTGGCTGCTCTTAGTATCTGTGGAAAAAATGATGAATGAAAGGAAGGAGTTGTGTGATAAAATC
    GAAAGGCTCCAGACACAAGTAAACGATCTAAAAGTTGCTAAGTGTGTCCTTGAGGAGAATCTTCTCTCTT
    GTAGCAATAGAGCTCAAGTTGCAGAAAATCAAACAGAAACTCTCATTGTAAGGTTGGCTGAACTACAGCG
    AAAATTCAAGTCTCAGCCTCAGAGTGTGTCAACAGTTAAAGTAAGGGCTCTAATTGGCAAAGAATGGGAT
    CCTACAACATGGGACGGGGATGTGTGGGAAGACCATGTTGAAGCTGAGAATTTTGAATCTTCAGATTCTC
    AAGGGTTTGCCCCACCTGAGGAAGTAGTACCCTCAGCCCCACCCCTTGAAATAATGCCTTCCCCACATGA
    GGAAATTAATTTTGCAGAGTCTGATAAACCAGCAATGATTTTCACTACTGATGTTTCTCAAGGCCCACCA
    ATAGTTTCTTCTAGACCTGTAACCAGACTCAAAGCAAAACAGGCTCCTAGAGGGGAGGTAGAAAGTGTAG
    TCCATGAGGAAATTCGCTACACTACTAAGGAGCTTAATGAGTTTGCTAATTCATTCAAGCAGAAACCTGG
    TGAATATGTGTGGGAATGGATTTTAAGGGTGTGGGATAAGGGTGGAAGGAACATAAGACTAGAGCAGGCT
    GAGTTTATTGACATGGGTCCTCTGAGTAGAGATTCTAGGTTTAATACGGAAGCTCGCATAATTAAAAAAG
    GTGTCAAAAGTTTGTTTGAATGGTTGGCTGAGGTGTTTATCAAAAGATGGCCTACTGGAAATGACTTGGA
    GATGCCTGATATTCCGTGGCTTAGTGTTGATGAAGGGATTTTAAGACTTAGGGAAATTGCAATGCTAGAG
    TGGATATATTGTGTAAAGCATAATTGTCCACAATGGGAAGGTCCAGAAGATATGCCTTTCACTAGCTCTA
    TAAGACGCAAATTGGTGAGAGGGGCACCAGCACATTTGAAGGGTTTTGTTCTTTCCCTTTTCCTTGTACC
    AGATCTTAGCATTGGAGATGCTTCTGCTCAATTAGATGAATTAAATTCACTGGGTTTAGTTGGATTCCGA
    GGTAACAAGGGCCAGGTGGCAGCATTGAATCGCCGGAGACAAGGTGATTCTAGTTATTATAATGGACAGC
    GTAGACAAAAGAATGTTTATAATAACATACCCAGCAATGGTCAGCACAGGAGAGGTGAAATTTATAATGG
    CATGACTCGGTTGGACCTTTGGTACTGGCTAACCAATCATGGTGTTTCCAGGAATGAAATACATAGGAAG
    CCTACTGCATATTTGTTTGATCTGTATAAGCAGAAAAATTCTCAAACAAATGAAAGAAAGGCTACATTAG
    ATCATGGTAAACAGCAATCTCGGCCAGTGAATCAATTTCCAGACTTGAGACAGTTTGCAGATCCAGAACC
    CCTTGAATGAAGGGGTGGCCAGGTTCCGCTGAGGAAGGATCTTGATAAGACACCCAAAGGTTTTGCTGTT
    ACCCTTTCTCCAGTTCTTCCCCAGAGGGACCTAAGGCCTTTTACAAGGGTAACTGTACACTGGGGAAAAG
    GAAATAATCAGACTTTTCAGGGTCTGCTGGATACTGGTTCTGAGTTGACACTGATTCCAGGGGATCCCAA
    GAAACATTGTGGCCCTCCAGTTAAAGTAGAGGCTTATGGAGGGCAGGTGATTAATGGAGTTTTGACTGAT
    GTCCGACTCACAATAGGTCCAGTAGGTCCCCAGACACATCCTGTGGTGATTTCCCCAGTTCCAGAATGTA
    TAATTGGGATAGATATACTCAGAAATTGGCAGAATTCTCATATTGGTTCCCTGAACTGTAGAGTGAGGGC
    TATTATGGTTGGAAAGGCCAAATGGAAACCTTTAGAGTTGCCTCTGCCAAAGAAAATAGTGAATCAAAAA
    CAGTATCGTATTCCTGGAGGCATTGCAGAAATTACTGCCACTATCAAGGACTTGAAAGATGCAGGGGTGG
    TGGTTCCCACCACATCTCCGTTTAACTCTCCTATCTGGCCAGTGCAGAAAACAGATGGATCATGGAGAAT
    GACAGTTGATTATCGAAAACTAAATCAGGTAGTAACTCCAATTGCAGCTGCTGTACCAGATGTAGTTTCA
    TTACTTGAGCAAATTAACACATCTCCTGGCACCTGGTATGCGGCTATTGATCTGGCAAATGCCTTCTTCT
    CAGTACCTGTCCATAAGGACCACCAGAAGCAATTTGCTTTCAGTTGGCAAGGCCAACAGTATACTTTCAC
    AGTTTTGCCTCAAGGATATATTAACTCTCCTGCCCTGTGTCATAATTTAGTTAGAAGGGATCTTGATCGT
    TTGGATCTTCCACAAAATATCACATTGGTGCACTATATTGATGACATTATGCTGATTGGACCAAGTGAGC
    AGGAAGTAGCAACCACTTTGGACTCATTGGTAACACATATGCGTATCAGAGGATGGGAAATAAATCCAAC
    CAAAATTCAAGGACCATCTACCTCAGTGAAATTCTTAGGAGTCCAGTGGTGTGGAGCATGCAGAGATATT
    CCTTCTAAGGTGAAAGATAAGTTATTGCACCTGGCCCCTCCTACAACCAAGAAAGAAGCACAACGTTTAG
    TGGGTCTATTTGGATTCTGGAGACAACACATCCCTCACTTGGGTGTGTTACTTAGGCCTATTTACCAAGT
    GACTCGGAAAGCTGCTAGCTTTGTGTGGGGCCTGGAACAGGAGAAGGCCCTTCAACAGGTCCAGGCTGCT
    GTGCAGGCTGCACTACCACTTGGACCATATGACCCAGCAGACCCGATGGTACTTGAGGTGTCTGTGGCTG
    ATAGAGATGCTGTTTGGAGCCTCTGGCAGGCCCCTGTAGGTGAATCACAGAAAAGACCTTTGGGATTTTG
    GAGCAAAGCTCTACCATCATCTGCAGACAACTATTCTCCCTTTGAAAAACAGCTCTTGGCCTGCTATTGG
    GCCTTAGTGGAAACTGAACGTTTGACAATAGGACACCAAGTTACTATGCGACCTGAACTACCCATCATGA
    GCTGGGTACTATCAGACCCTGCAAGTCATAAAGTGGGACGCGCACAGCAGCAGTCTGTTATCAAATGGAA
    GTGGTATATACGTGATCGGGCCAGAGCAGGTCCTGAAGGCACAAGCAAGTTACATGAAGAAGTTGCTCAA
    ATGCCTATGGTTTCTACTCCTGTTACACTGCCATCTGCTGCCAAACATGTGCCTATAGCCTCATGGGGTG
    TTCCCTATGATCGACTGACCGAAGAGGAAAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTTATGC
    AGGCACCACCCAGAAGTGGACAGACAGCTGCAGCATTACAACCCCTTTCTGGGACAACCCTGAAAGACAC
    AGGTGAAGGGAAATCTTCACAGTGGGCAGAACTTCGGGCAGTACACATGGTATTACAGTTTGTTTGCAAG
    AAGAAATGGCCAGATGTACGATTATTCACTGACTCATGGGCTGTAGCCAATGGATTGGCTGGATGGTCAG
    GGACTTGGAAAGATCACAATTGGAAAATTGGTGAGAAAGACATCTGGGGAAGAAGTATGTGGATAGATCT
    CTCCAAATGGGCAAAGGATGTGAAGATATTTGTGTCCCATGTAAATGCTCACCAAAAGGTGACTTCAGCT
    GAGGAGGAGTTCAATAATCAAGTGGATAAGATGACCCGTTCTGTGGACAGTCAGCCTCTCTCCCCAGCCA
    TCCCTGTCATTGCTCAATGGGCACATGAACAAAGTGGCCATGGTGGTCGAGATGGAGGTTATGCTTGGGC
    TCAGCAACACGGGCTTCCACTCACCAAGGCTGACCTGGCTACAGCTGCTGCTGATTGCCAGATCTGCCAA
    CAGCAGAAACCAACACTGAGTCCCAGATATGGCACCATTCCTCGAGGTGACCAGCCAGCAACCTGGTGGC
    AGGTTGACTACATTGGACCACTTCCTTCGTGGAAAGGACAGCGTTTTGTTCTTACTGGAGTAGATACTTA
    TTCTGGTTATGGATTTGCCTTTCCTGTACGTAATGCCTCTGCTAAAACCACCATTAACGGACTGACAGAA
    TGCCTTATCTATCGTCATGGTATTCCACACAGTATTGCTTCTGACCAAGGAACTCATTTCACAGCCAGAG
    AAGTACGACAGTGGGCCCACGATCATGGAATTCACTGGTCTTACCACATTCCCCATCATCCTGAAGCAGC
    TGGTCTGATAGAAAGATGGAATGGCCTTCTGAAGACGCAGTTACAGCGCCAATTAGGTGGTAACAGCTTG
    GAAGGCTGGGGTAGAGTTCTTCAGAAGGCAGTATATGCTTTGAATCAGCGCTCGATATATAGTACAGTTT
    CACCCATAGCCAGGATTCATGGGTCCAGGAATCAAGGGGTGGAAAAAGGAATAGTTCCACTTACTATCAC
    TCCTAGTGACCCTCTAGGAAAATTTTTGCTTCCTGTCCCCATAACTCTAGGTTCTGCTGGCTTAGAAGTT
    TTGGCTCCAGAGAGGGGAGTGCTCCTACCAGGAGCTACAACAAACATTCCATTGAACTGGAAGCTCAGAC
    TTCCCCCTGGTCATTTTGGGCTTCTAATGCCCTTAAACCAACAGGCTAAAAAAGGAGTAACAGTGTTAGG
    AGGGGTGATAGATCCAGATTACCATGGGGAAATTGGATTACCTCTTCACAATGGTGGTAAGCAAGATTAT
    GTCTGGAGTGTAGGAGATCCCTTAGGGCGTCTCTTAGTACTACCATGTCCTGTGATTAAAGTCAATGGGA
    AACTACAACAGCCTAATCCAAGCAGGATGACAAAGGACACAGACCCATCAGGAATGAAGGTATGGGTCAA
    TCCTCCAGGAAAAGAGCCAAGACCTGCTGAGGTGCTGACTGAAGGAGAAGGAAATATAGAATGGGTAGTA
    GAGGAAGGTAGTTATAAATACCAATTAAGGCCACGTAACCAGTTGCAGAAACGAGGATTATAAAGTAATA
    TGAATGCCCATTGTAAATTTACTAATGCGTTTGCGATTGTACGAGGGATAGTTATATCATGTTAGGCGTA
    TTTACAAACTTGTTATTGTTTTATGTGAACATGAGATATTATTTGTGTCAAGTTGACAAGGGGTGGATTG
    TAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGGAAGGCTCAC
    CAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAGCCATAGTGG
    CTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACGCCTTTCATC
    TGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAATATAAGGAC
    ATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTGAGTAACTAC
    TAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGACTCTAAGTC
    ATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTGACTAATACA
    TCCCTACATACAGGAAACTTGATGTATAAGTAAAGAAAATAGTAAATCTAAAAATTTCCTTATCAAATAC
    ATGCAAGAAATCCAAAATACCATGAAAAGACCTGATCTAAAAATAGTAGGAACTGAAGGTGAAGTGTCCC
    AGCTCTGAAAACTAGAAAATGTTTTCAATATTTTTCCTAACAGTGAAGACATGGATCTAATGAGGCCACC
    TCTTATAGCCATGCAAGACTGACAGTGGAGGGATAAGGACACCAATCCACCCACAAAACTTTTGACCCTA
    AATCAGTTTTATCTAAAAGCAATGCAGGGGCACAAATGGAGCAGAGACTGGAGGAATGGTCAAACAATAA
    CCTGTCATATCTGAGACCCACCTAATTGGCATACACCAGTCACTGACATTATTAATAATGTTCCATTATG
    CTTACAGGCATTTCTCTAGCATTACTATTCTTGGAGAGACTTCACACAGCATCTTATTGAAACAGATGCA
    GACACCCAGAACCAAACATTGGATGGAGATTGGGATCCTTAAAGAAGAGTTGGGAGGATAATTGAGAGAC
    CTCAAAGGAATAGCAACCCCATAGAAAGACACGAACAGGGTCAGTAAACCTGGACCCATGGGGTATCTCA
    GAGACTGATCACCAACCAAAAATCACAGAGGGCCTGGATTGATTCCCCTGGCACACATGTAGCAGAGGTC
    TGCCTTGTCCTTCAGTAGGTGAAGATGAGCCTAAGGCTTCAGAGACTTGATATATCAGCATACAGCAACA
    CCCAAGTGTGTCCCACTATCTCAGAGGTGAAGGGGTGGAGATGGGCAGAAGGGTCCTGATAGGGGACAAC
    TGCAAAGAGGCAATATTTGGGACAGAAAGAAAGGAAGAGAGAGGAAGATAAAGAGAGAGATGAAGGAAGG
    AAACAAACAAACCAAGATGAAGAGAGAGAAAAGGGGGAAAATAGCCTTGAATGGTTCAGGGATTCAAAGG
    GACATACCTAAGCATGGTAAAACAATGCACATAATGGCAGCAAGTAGCTAACACTGAATTAAATAGAAAG
    ACACATAAAGCAGTTCTACTAAAGTAAGAGACAGAGTTGCCCAATCTCACCCTATTTATTCAATAATAAT
    GTACTTGAAGTTCTAGCTAGGGCTGTAAGACAACTCAAGGAGATCGGAGGATACAAATTAGAAAGAAAAA
    TTCAAAGAATTGTAATTGGTAGATGATAGGATAATATACATAAATGACCTCAAAATTCTTCCAGAGAACT
    CTGAAAGCTGATAAATACCTTCAGCAAAGTGGCTAGAAACAAAATTACTTCATAGAATTCAGGAGCTATC
    CTTTATAGAAAAGTTAAAGTGGCTGAGAAAGAAATTAGGAAAATCACATTCTTTGCAATAACCAAAAATA
    ATATGAATTAGCTTGGTGTGACTCTAATCTTGCAAGTGAAAGATGTGTGTGACAAGAGCTTCAGGACCCT
    GAAGAAAGAAATCAAAGAACTCAAAGATGCAAAGTACTCTAATGCTCATGGATAGGCAGAATTAACATAA
    TGAAAATGCCAAATTTAACAATTCAATCTACAGATTCAGTGGAATTCCCATTAAATATCCAACCCAATTT
    ATTACATTCTTGAAAAAGCAAATCTCAACTTCATATAGAAAAACAAGAAATTGAGGGTAGCTAACAAAAT
    CCTGAACAATGAAAACACTTCAGGAGAATTCACCATCCCCTACCTCAAGCTGTATTTTAGAGCAATAGTT
    ATTAAAACTGCATGGTATTTGTATAGAAACAGATATGATGATCAATGTAATTGAATTGAATACACTGACA
    TAAAACCACACTGTTATGGACACTTGATTTTTGACAAAGAACCCAATAATCATAATAAAAATCATAATAA
    GAATGCATCCCCCAACAAATGGTCCTGATCTAAATGCAAATAGATTCATATCTATCATCCTGCACGAAAT
    TCAAATCAAAGTGAATTGCAGACTTCGACCTAATACTGGATTAACTAAATCTAAATGAACAAAAAGTAGA
    GAATAGTTTTGAACTCATTGATGCAGGATTCAATTTCCTGAACAGAACCACAATGGCTCAGGCTCTAGGA
    TCATAAATTGGTAGATAGGATCTCATGAAACTGAAAAACTTCTGTAAGGCAAAGGCAATAAAACAAAATG
    GCAACCTACAGATTGGAAAAAAAATAATCTTCAGTATCCCTAAATCCCATTGAAGGCCAATATGCAAAGT
    ATATAAAGAACTCAAGATGTTATCCTCCACAAAACCAAATAAATCAATTAAAAATGAACTACAGAGCTAA
    CAAGAGAATTCTCAACAGAGGAATCACCAATGACTGAGAATCACTTAAAGAAACATTCAATATTTGTAGT
    CACCAGAGAATTGCAAATCAAAACTACCCTGAGATTCTACTTTATACCAATCATAATGGTCAAGATAAAA
    ATTCAAATGACAGCGCATGTTGGTGAGGATGTGTATACTTTTGCATTGCTGGTGAGGAAAACAATCTGGC
    AGCTCCTCAGAAAATTGTAAATAATTCTACCTGAAGATCCAGCCATACCACTCCTGTGCATATACATAAA
    ATGTGCTCCACCATACCACTAGGAGATATGTGCCACTATGCCCATAGCAGCCTTATTTGTTAATAGCCAA
    AAGATGGATACAACCCTGATGTGCCTCAAACAAAGATTGTATATAGAAAATATGGGTTCCCTTACACAAT
    GGTATTCTACTCAGCAATTAAAACGTGAGAACATCATAAAGTTTTCAGGCAAACTGATCAAATGAAAAAG
    TATAATCTTGAGTGAGGTAACAGAACCAAAATGACCTGCATGGGATGTACTCACTGGTAAGTGAATATTA
    GACTAAAAGTATAGAATATCAATGATGAAAGCCACAGACCATAAAGAGTTTAATAAGATGGAAAGCTCAA
    GTACGGATTCTTCAATCCAATATAGAAAGAGGGACAAAATAATCAGGGGAGGCAGAGGGAGAGAGAGACA
    TTTGGTGGAAATGGGAGAAGAAGGGAGAAAGGCAGAAAGGATCAGGCGTTGGGGAACACTGGAAAGCAGC
    CCAGGGGTCCTGGTAAATGGATTAAATATTCAGCTGAATGGAGGTGGGAGGCAGGGGGAAGCTCTGCAAA
    GTCCAAGAGACCTGGGATGTGAGAGGCTCCCCAGATGATAATCTTAGCCTTCATGCCTAACAGTTGTAGA
    TAAACCCTGAAGAGATCACTTCCAATAGATGCAGAGGGCCCTAAGTGGTTGGATGGAGTCCCCCAACTTA
    CCTCAAAATTGTCAATACTCATAGAAATTAGGACAATGAAAATCAAAACACCCTGAGATTGTATCTTATG
    TCTGTCACAATGGTTAAGACCAAAACCTCAAGTGATGGCTTGTGCTGGCAAAGATTCAGAATAGTAAAAG
    TCTCCCCATGGCTTGTGGGAATGCAAACTCTTACAACCTCTTTGGAAGTATATTTGATTGTTACCTGGTA
    TAAAATGGCAAAAAAAAAAAATGGTTGAAGGGGGACTAAAAAAGGAAGAAAGGGGAGAACTATGGGATGC
    GAAACAAGAAAGTTTGTTGCAAAAGAAATATGTTTCCACTGCAAACCCTGAGTCTCAGACAGAAGGGGAC
    CTGGAATTCTTCAGATACAAAGAATCTCTAAACCCTGAGGACATTCTATCACAAATAAGTAAAATTCAGA
    AAATTCTGAGTGCTCCCATCACGGAGATGAATCTGCTATGAACAGCTCATAGGTGTGACCCTCTACAAAA
    GCCATATTATTGAAAAGCCACATTGTGCCCAGACTTTGGAAAGACTGAGCTCATATCCTGAAATACAGTT
    ATGTGTGGTTCTATTTAATTACACATTTACACTAAGAAAACATGGCAGTATGGGAATGAAGCTTGTTCTG
    TACACATTAACAGAGGGAAACTAAACAAAGTATGGTGAATCCCTAACCAAAAGTAAAAAAAAAAAAAGAA
    AGAAAAGAAAATAAAAGTGAAACTACAATATGTTTCAAATGCTGTAACTGAAATCTGGTTTTTTGATGCT
    TATATCTGGTATCATCAGTGACTTCAGATTTAGTCCAACTCCAGAGCATGGTATAGCAGAAAGACATGCA
    AATAAGCCTTCTCTCTGCCCATGAAAAACACCTCGGCCCTGACCCTGCAGCTCTGACAGAGGAGGCCAGT
    CCTGGATTCGATTCCCAGTTCCTCACATTCAGTGATCAGCACTGAACACGGACCCCTCACCATGAACTTG
    GGGCTCAGCTTGATTTTCCTTGTCCTTGTTTTAAAAGGTAATTTATTGAGAAGAGATGACATCTGTTGTA
    TGCTCATGAGACAGAAAAATTGTTTGTTTTGTTAGTGACAGTTTTCCAACCAGTATTCTCTGTTTGCAGG
    TGTCCAGTGTGAAGTGAAGCTGGTGGAGTCTGGGGGAGGCTTAGTGCAGCCTGGAGGGTCCCTGAAACTC
    TCCTGTGCAACCTCTGGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCAGAGAAGA
    GGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTATTATCCAGACACTGTAAAGGGCCG
    ATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCCGTCTGAAGTCTGAGGAC
    ACAGCCATGTATTACTGTGCAAGACACACAATGAGGAAATGTTACTGTGAGCTCAAACTAAAACCTCCTG
    CAGAGCACCCAGGACCAGCAGGGGGCGCAGAGAGCACATGGAGTTCTGATTCACAGAAGAGTTACAGCCT
    GTACAATTAGACCCAATCTTCAACAAACCGTCAAAATATTCGATCCAAAATTGTTCCTGTCTAAAAGTAA
    TTCAAGGACAAAATGGACCAGAGACTGAAGAAATGGCTGACCTGTGACCCTCCCAACTTTGGATCTATCT
    CATAGGCAGGTACCAAACCTTGACATTTGTCACTGACACTGTATTGTGCTTGCAGACAGGAGCATAGCAT
    GGCTGACCTCTAAGAGGCTCTGCAAGCACCTGAATGAGACAGATGTAAATAGAGTGTACAACTGGATTAA
    GTTTGAAGACTGCAATGGAAGAGTTAGAGGAAGGAATGAAAAATCTGAAGGGGATCACAACCCCAGAAGA
    AGACCAACAGTGCCAATTAAACTGGATGCCTGGGAACTCCCAGAGACTAAGCCACAAACCAAGAATCATG
    CAGGCTGGTCTGAGAATCCTGGCACCCAAGTCTCAAGGAACTGCCTTTTCTTGGCTCAGATGGGAGAAGA
    TGAGCCTAAAGCTTTAGACTTGACGCCCCAGGGATGGGCAATACAAAGTTGAGTTTCTTGGGAAAGTGAA
    GAGGATGGTGGGAGGAGGAATAAATCTGGGAAGTGGGACTGGTTGGGGACAACATATTGGTCCTAAATTT
    ATGAAATAATTATCTAATTGATAAAAAGAGTCCTTGGGGTAATGGAGGGCTAGACTCCTCTGTGCCTAGT
    TTGTAACTCTACAGGGATCCTCTTTAAAAGAATAGGGTGCACATAGAGTATATGTGTGTGACACTAATAC
    AGGGGTAAGTGTTTCTGTAAGAACTTTATGAATAAACTTTATTAAAACATCAAAAAGTATAGGTTGTAGC
    AACCCTTGACCTGTACGAATGTTTATAAAACTTTCTATTTTCCTTAATTATATCTGTTTTGCATTTGTTT
    ATTTATTTATTTACTTACTTACTTATTTATGATTTCACTGTGTCTTTCTGTAGTCCTGTATGTGTTTATT
    TGTCTCTTTATTGCTCTGTTTCTGTTTCTTTCTCTCTGTCTAGTATTGTATCTCTCTGTGTCTCTATGTC
    GCCGATTGTGTCTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATATTGAAA
    TGAGATCTCTTCTTCTATCAGTGTACATAATGACTTTGAAATAAAGTCCATCACTGGGCTTGGATATCAC
    TATCTTGTTAGAATGGTTGCCCAAAGTGTTCCAACCATCACACTGTTTCTACATATAATAGATGGGTTTA
    TTGATTTACTACCAAAACTACCCCATCTTAAGTTATGGATATTAAACACAGATCCTCATGGTGTGTTAAG
    AACTTGAAGCACAGAGCTATATGTACAGCCCACTAAAAATGACTTAAATATAAGATAAACTCTGGCTTGA
    ACAAAACACAGAGCATCACACGAGGCAGAAGTGAATCTCTATGAGGACCAGGATCACATCCCAAAGAACC
    AAAGAACACTTGAGACTGTGGAACCCTAGAGTCAGGGCTGACACTCAGTAAGATTTACTTCATAGAGCAT
    GATGTGGGATTCAAGAAGAAATCAGGCAGGGCCTGTGACCCATGAACCAGATGACTTCCAGTCTCTCCAT
    CATCCTCACACAAACCATAGGCAACAGCTCAGCACTGACTCACCTGTGATGCTGGTGACAAAATGTGTGC
    AAAGCCTCAGAACTGAGACGTGAGGCCAATGTTGCTACCGACACTCAGTGAGCTTCAATTTGCTGCAAAC
    TGCTCCAAAACAACTCAAAGGGTAACTTCTTCTTTTTCTATTTGTACTTGTGATGCCTTGGTAGATATAG
    TTCCCCAATAATTTAATAGGACAAATCATTTTTCCTTTTAACACATTAATCCCCTTTCCTTCCCCCAAGA
    TTCTAAAATTTTTCTTTAACACTGATGTCTGAGAGATATGGCATAATAGATGTTTGTCTTATTCTGAGTT
    GTTCCTTATCAAAAGAAATCAGCTGTGGCACTTTGAACCTAAATATAACAATATTTCACAAAGGCATTCT
    GGGAATATATAGATGGTATTGCTACAAAAGAATAGGAGGATCTATGGCCATACCACCCTTAATGAACCAG
    ATACTATCTAAAAGATTAGGACAGCTGGGCATGTTGGCACAGGCCTTTAATCCCAGCACTAAGGAGGCAG
    AGGCAGGCAGATTTCTGATTCGAGGCCAGACTGGACTACAAAGTGAGTTCCAAGACAACCAGGGCTATAC
    AGAGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAGAGTAGGAGGGTATTAGATGCGAGGTGAT
    GATGGCCATTCAATCAAACCAGAATGTGACCTGAGAAAAGGTCTAAGTTTGTCAGCTTGTCCTGGAGATG
    TGTTGCCTCTGATAAATGCAGAGCTGGAACTGCAATACATTGGATTCCTGTGAGTTACGGAGAAATAAAA
    GGAATAGCCTCTGTTTCCTTTGGTTCATCAAGTTGGCAGTGAGCTAGAAGATGATACTGGGAACCATCAC
    ATGTACTTCTACAGAGTTATTCTACATTCTACAAGTCAGTCTTGCTTGCTTTCGTTTCTGTGAGGAGCTA
    AATACAGAATGTTTTCAATGTCTCATAATGTTCTGTGCACACCCATGGGTGCCTAGCCAAGGATAGAAAC
    TTGTATCTGATAAGGAAAGAGGATGCTGCAGTGGCCACACTCTGAGATCACCTCCTGCCAAATTATACTA
    GAGTGTTTTACATAGTCTTGAAAACACTAGGTCAAATATGTTGTCATCCAGATATGAAAACTCTGTGCAA
    CAATTCAGAAATACAGTGGTCAGGACAACTGGTATGTAACACTTTAGTTCCAGCCCACATTAATCTCACA
    TCTTTTCTGTGGTTTCAATAAAGAGCACACATGTTTGGAACACCATCTCCACTTTGTATGCTTATCCTTA
    TGCATCAAACTTTTCCTGTAATTTCCTACCCCGCACAGGTTCCTGTCTTGATCTCTTTTTCTAATGAACA
    GCAATACAGAAGTGTTGGCCAAATGAACACTTTCACCCCAAGTTGCTTTGCCATTGTGTTTCATCACAGC
    ATTAGTAACTCCCAGTAAGGCTACTGGTTACCCTGCATCTTCTATGTAGATCTTTTGATGAAGTTCCAGG
    CTTGGGGTCTTTATCTGACTGTTATCCTGGTTAATTCATGTCCACCAATAGAATATTGTTCTCATTTTCA
    ATATCATATGAAGTTACCTGTTTATAGGATCTTCCTAAAGCAGAAAACTATATAAAATGTCTGCTTACTT
    TAAGAAAAGTATGTGTCCTTGGAGGTAAATGGAGGAAAGGGTGGAGGATGTTGAGTAATGGAAGACTGGC
    TAATACAGGAAGGTCACAGAATTGAATGATTTTAAAAATTTTAACTCCTCATCAATGAGGTACTTCAAGT
    TGAGGCAGGAGTCCTTGATGCACAGGTGACCCAAAGGCCCCAGCCTCTGTTTCTTGGTCTGCTCCAGTCT
    CCCGGCTCAGCACAGTTCACTGAGTGACAATCTTTAAGATAGTCTTTCAGGATACAGTTATCACAGTTTT
    CAAACTCTCTTCATTGAAACAATATAATGAGTCAGAGTCCAGTGTGCCATTAATAGGCCCTATGCAATAG
    GATTTTACTTCATCTGTGGAAAGAAACCACCATGGCCTCATCAAGGCCTCATTTGATGATTAGTGTAGAA
    AGCGTCCCCAGAGCTTTGCTGGGATGAAAACCCTGGCAAGATGTTTAGGGCACTCTCCATCTGACAGGAC
    AATTCTATCAAGGGTTGCTTCCATATAGAGAGAAATCTCCACATCTGCAGTATTTCCCGATTTATCAACA
    GTTCTTTACCCCGTGTCATATGCATAAAATCTTTTCTCTGTAGAATTTTCAGTACTAAAAACTAACAAGG
    ATGTTGGTCCTGATATCAGACATAATAAATAAATAAACATCAAATTATACCCTAAACATGTGGAGATGGT
    TTTCTGTTATGAATTTATTGGCTCTCTAAAAAGTGTTAATTAAGCCAGGCGTGATGGCACATGCCTTTAA
    TCCCAGCACTTGTGAGGCAGAGGCAGGCGAATTTCTGAGTTTGAGGCCAAACTGATCTACAGAGTGATTT
    CCAGGACAGCCAGGGTTACACAAAAAAACCCTGTCTCCAAAAAGTAACAAAAAGAAAGTGTTAATTAACA
    TCTACACTTTAAGATAATTTGGTGGCAAAGGTTAATAGTCTGAAATACTAGATATGAAAGTATGACACCT
    AAATTGCATGCAGAGAATAAAGCTGAATTCCAATGAGATTCATGCCTTGTGAATCCATTCCAATCTGATC
    TCAATCTTCAAACATGTTTGTGCATAATTTACATCTTAATCAGTTGCTGGAAATTAAAAGGACATAAGCT
    AAACTTAAAACTCTTTCTATTTAATGTGTCAGAATAGAATATGTGCACTGAAGTTTTCATGTGCATAGGA
    TATCATTGTTACAATGATGGTTCAAATACGCACCAGTAGCACAGGTGATGCTTGACATGAAAAAAACTGT
    GACCAGAGCCCAGACGTTGATGTTGGCTGAAAGAGCCTAGTGTCTCACATTTGATCAAGCAAAGTGTTAT
    TCAGAGAAGCAGTGCCAGATTTGGGATCCTTCTTAATGGATCAAGAACATGAGGAAGATTTGGAAGCCAG
    ATTGATGAGATTACTAGAATCCTGGATCTGAACACACAACACACAATAGTAAGTTGAACTTTTCCACGCA
    ATGCTAAAGATAAAGCATCCAGTATCATGTTATACAGGACCAAGGTGAGGATCAGTCTGTGGGTGAAGTA
    CAATGAACTGGAAATTGTTTGGATTTTAAATGTTGGTTTTTAAATTATATATAAAATATATATATATATA
    TATATATATACATATATATATATATATATATATATATATATATATCGGAACCAACATTTAAAATTTGGAT
    TTTAAATGTTGGTTTTTAAATTATATATAAATTATATATATATATATATATATATATATATATATATATA
    TATATATATATATATCGGAACCAACATTTAAATTCCTAATTCTTAAGGACTTCACTTTTGACCAAAAATA
    TTTGAAATGGGTTATTTAGGTCTGTGGTGGTTTGAATATGCATAGCCTATAGGAAGTGGAAGTATGGCCA
    TTTTGTAGTATGTGTAGCCTTGATGGAGGAAGTACATCACTATATGGTGTTGTGAGAGTTGAGATCCTAT
    GTTCAAGTTCTGCCAAAAACAGAAGAGAGTCCCCTCCTGGATATTTGAAACAGATGCAGAGGCCCAAAGC
    CAAACATTGGGTATATCTCAGGGTTTCTTATGGAAAAGTTGGCTGAAGGATTGAAGGCCTTAAAGGGGAT
    AGGAACTTCACAGGAAAACCAACAGATTCATCTAACCTAGACTCTAGGGGTCTTTCAAAGGTTGAGTCAC
    CATACAAAAAGGACACAATGGCTAGACCTAGGTCCCTGATACATATGTAGCAAATGTGCAGCTTAGACTC
    CATGTGGATTCCCCAACGACTAGAGCAGGTATTGTCCCTGAAGTTATTGCTTGTCTGTAGAATACATTTC
    CCAACATAATTGTCTTGTCTGGCCTCAGTAGGAGAGTATGTTCCAATCCTGGAGAGACTTGATATGCCAG
    GATTGAGAGATTAAGGGTCTCCCACCATCTCAAAGTTCATGGGTATGACAGGGGAGGGGCAGTGCAGGTT
    TTCCCCATCTCTGATTTCCAGCATTTGCACCCCCAATATTGAGGGGGAGGTCTAGCAATTGGAGGGGGGA
    CAAACAGTATGAGAAAGCTAACTTTAGTGGTGTTGTCACAGTGTGGGGTATTGTGGGTGGGAGTGGAGAT
    GAATCCTCCTCCTCCTCAGGAGGCTGTAGCAGGTGCTTGCCCTCTTTTGGGACAGTAGAGCTGTGGTCAG
    TACTGGCCTGTTCCTGACAAATTTATTCTGTGCTGTGACCATTTATTCAAGGGAGGAAGATTTAAGACTT
    GCTATGATAACCTGACGAATCTGGACAAACAAGAGACAGGAGGTAACACCTGAGTTACTCTTTTGCTTGC
    TAGTACAGAAGAGCACTATAATCCACAAATTGGGGTCCCACATATTCTTGAGTGGGATCCAAGGTGCCAT
    AATTTCAGCCACATTCCAAAGTCCCTCAAGGTCTGACAGTTTAGTCTTTAGGCTTCTCCTAACCAAATCA
    TATTTGGAGTTCTTTAACATAAAAGGGTAGTTTCCCCATATCACTGCCAGCAAAACAGGTTGAATTTGGA
    CAGACAAGCAAAACTATCACAAACAAGACAGACTATGTGTGCGATCCAAATGTCTTGTGATTAGAGGGGA
    AGTTCTGCAGCTTATTAGGCTGTGTCCTTATGCCCACAAATGTCTTCAGATAGGAAAGGGCCATTGGGTG
    CCAATCCTTAGTTCTTCTGTTTCCTGGGCTGTCAGTTTTTATGTGGAGAAGACATGAACCAAATGCAAAG
    TCATGTATCCTACAAGAACTGACATACACAGACAAACAAGGAATAACAATGAGCTCTCTCATTCACCTAA
    GGGGGTCTTATAACATAGAACAGAAAGGGGCCAACTTAGCAAGAATTGACCAAAAGGTGGTCACCTAGAC
    CACACCACAATGCAGGAAGGATATCAATGAAGGAAGAACAAAGGAAAGGCCAAATCAAGTTTGTTGGCAC
    ATGACATAAAACAGTGCTCAAAAAACAATAGATGCGAGCAAAATGAAGCATTGGAATCATATCGGGGTAC
    ATAGGGGTCACTCTTACCCCTGTTCAAGTGCCAGATGTTGTTTTAGCCTTAGGCTCCCTGAGTTTGTCTT
    TAGGGGAAATGGGTAAGAATACACAAAAATAATGTCACAGAATCTTAGAGGCAGATGAGAAGTCAAAGCA
    GACTCATTTATTGCTATAGAAAAGAATGCCTTTATACCTTCTGTCCATAACACCTCTGCCCATATATGCT
    CATCTCTGTGAGGCAATGGACTACCTGCAGACAGGCTGGTATCCAGTCAAGCTGAGGTCTGAACCACAAT
    GGTCGTAACTCACCTAAAGGACGTGGTAGGCATGACGCGATAGGCGTTCCCTCATGCTCCTGGAACTCCG
    GCCCCTGCCTAAGGTACCACCTCCCACAGCCCCCACAAGAGAAGCATGGTCAGTAGTCATGTAAGCAATG
    GCCCAAGCTTCTGACCTTCAGCCTAAACTCCTCCCCAGTTACCTAGCAACAGTGAAGACCATAAAATGGG
    ATGTTAGGCCCCCACCTCACTCTCTTACTCCTTTGTTCCTTTACCTCTCACTTCTCTCAATTCTCTCTCC
    TCTTCCCTTTCTTTGTCTTCTTCTTTCCTTGTCTCTCTGTCTTTCTCTTTCTCTCTAGCCTTCTCTGCCT
    CTCTCTCTCTCTCTGTCTCTCTGTCTCTCTCTTCTCTCTTTCCCTTGAATTTCTTTTTTTCTTTTTTCTT
    TTTTTGATGAGCACAGTATAAATAGTTTATTGCATTGTTTCTGAATGTGGCTGTATTCTCTATTGTTCTC
    TTTCTTATTTTTTTTCTTTTTTTGGTTGTTTTTGTTTTTATAGTCTCGCACAGAATTCTACATCTACCAA
    ATCCCCATTGTGTACTTTAGTTTTGTCTTGTATTCAAAGGTTCTTTCAGAAAGAATCTTAAAGATGAAAT
    TTGCCTTCCAACTTTTCAAAAATACATATAAAGATAGATGTTACATAGTTCACGGAATAGACACATATAC
    GGAGATGCAGCAATATCTCCTTTGTGCCTCTTGTATATTCATGTTATTTCCTCAACTAAAAATACATAGC
    CACAAAGACATACAATGAGCACTCCACTTAGGAAAATATGGCCATATTTTTTTACATTTTTATGATTTTA
    TGATTAGGTATATTCTTCATTTACATTTCCAATGCTACCCCAAAAGTCCCCCAACCCTTCCCTCACTCCC
    CTTCCCCACTCCCACTTGTTGGCCCTGGAGTTCCCCTGTACTGAGGCATATAAAGTTTGCAAGACCAATG
    GGCCTCTCTTTCCACTGATGGCCCACAAGGTCATCTTCTGATACATATGCAGCTAGATACATATACATAC
    ACGAGCTCCAGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTATAGGGTTGCAGATCCCTTCAGCTC
    CTTGGGTACTTTCTCTAGCTCCTCCATTGAGGGCCCTGTGATCCATCCAATAGCTGACTGTGAGCATCCA
    CTTCTGTGTTTGCTAGGCCCCATTATAGCCTTGCAAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTT
    GCTAGTGTGTGCAATGGTGTCAGCATTTGGGGGCTGATTATGGCATGGATCCCCGGATATGGCAGTCTCT
    AGATGGTCCATCCTTTCATCTCAGCTCCAATCTTTGTCTCTGTAACTCTTTCCATGGGTGTTTTGTTCCC
    AATTCTAAGAAGGAGCAAAGTGTCCACACATTTGTATTCATTACTCTTGAGTTTCATGTGTTTCCCTTGC
    ATTTCTATAATAAACCATAAGGAGTCTCTGCTCTACCAAGACCCGCTGCACACTCTGGTCAGTGTTGGGA
    ACTTTTCCCCTATTCCCTCTCTCCTATAACTCCGGGGCTACAGGGTGTCTCCTTTGGGTCCCGGTTGGGA
    GCTGTCTCTTCTCAACCCCCTGACTCATGGGTCAGAGGCCTAAATCTCCACCCAAGGCTGTGTGAAAAGC
    ACCGGGTGGTCTCCCCATATCTCCCTGTCCAGAGCACAGGAACTCTGGCCGGACATGGCATATTTTTCCT
    CCCCAACTTCTTCCCCGGCCTCCTCAGGGCTGTCCCTTCATTTTGTTCCCCACACATCTCCACATGGCTG
    CCACCATCCAATTGGCTGCTGAGGTCACACACTCTGTCTTTGTTCTTCTCTGAGTCTCATGCAGGTTTTG
    TGTTAGTAAATGCAAAAGTGCCTGCTGTGCATGCATGAACCATTACAGGCTGCTAGGCAGAACATGCTGA
    CTTGCTCCTGGATTATAAATCCATCACTGTGATAGCAGGACATATCCAGAAGACATTATTTAACAACACT
    CCTCTTCGTGCTCAACCTCTCATGGTCTTTCTACCTCCTCTTTCTTAGTGTTCTCTGAGCCTACATGAAA
    TGTGAACAAACCTGAAAAAAATCTCTGTGTCAAATACATAGTGTGGATTGGGAAGGAAAAACCCTGCACT
    TGTGTGTGGGCAGGGAAATGCTGGTGCTTTAACAGGACAAGTGCGGTGTGGCTAGAGAAAGTGATGAGAA
    GAAGGGAAGCATGAGAACTGGGTGGGTGGATCCCATGTCTACTGAAAATGCTATGTTGATGTCAAGATAT
    TGTGTCTTGTTACAAAAATTCATTGAGTACCTAGGTTATTCTCAGTATATAAAAGGGGAGGAATGAAACT
    AGAATTAAACACACACACACACACACACACACACACACACAAACACACACACACGAGTAGAGAAAAGAAA
    TAGTCATGAGTTGCCTAGTGAATGAAGAGCGGAAAATGGTGCAGTTAATAGGGATCATAGAAAGAACTGG
    GGGATGCACACGAATGGGTTTCCTGTAATTATGTCTTCATAGTAATCTCTGCTCAATAATCAGACAGTGA
    CAATGTATGCCTCATTTACAAGCCCTGATGGCCTCTCAGAGGAAAGCATCTCTCTGAAATGAATAAAGTT
    CAGAAATGTCCTAAGTGTCCCTGTCACAGGAAAGCAGTGTTGGTAACGTTTCCAGGAAGCTCAGTCTTTG
    TCAAATATCCACAACAAGAAGAAGCCATGTCTAGACAGACAACAGACTGGGAAAGACTGAGCTCATGTAT
    GGAAACACAACTATGTGTCATGCTTTTATTTTCACATTATACTGAGGGGATATGACATTATAGAAACACA
    GTTTGTTCTTTGCATATGCTGAGAGAGGAACTAGATGAAGTGTGCTAATTCTTAGCAATAAAAAGATATA
    CAAAGTGGTACTTTACTGTTTCAATTTTTGTAACTGGCATCATTGTCTTTGATATTTTATATCTTCCTTG
    AGCACTGCCTTCTCTCAAGTGTCCAACTTCAGAGTATGCTATAGCAGGAAGACTACCAAATAAGATTAAT
    TTTTTTGTACCCATGAAAAAATTATGTGCCCTGACCCCTGTTCTCTGAAAGAGGAGCCAAGTTCTGGATT
    CCCAGATCCTCATATTCATTGATCAGCAGTGAACACAGATCATTCACCATGGACATGGGGCTCATCTGGA
    TTCTCCTTATTGTTTTTAAAAAAGGATTTCATTGGGAAAAGCTGCCTCATATTTCTATGACCAGGAGAGG
    AAGATACAGCTAGAGACACAAGCCCCACCATGTAAAATCTGTATGGTACTCTCGTTTTCATTTTACAACA
    TTTTGCTTTTGCATTGCAAAGGAGCAATAAGGGTTCAGCAATCAGCACTGTAACTGTACTTTTAAAATTT
    CCACCTCTACCCTCTGTGTCCTCTATCTAGTGATAGAAAGTGAAATAAGCACATAAAGCATGGGACAAGA
    AGAACACACAGAGGAGCAGAGACTGGCTACAAGTGGCAGCCAGCATGGAGCTGGACAGAATTTAAAAAGA
    TAAAGAGAAATGTCATGGCATGAAGCAGTGACTTTTTCTGTTCACAATGCCTGCAAAAACTTTATGGAGC
    CTACTACTGCTGAGATATGCAGGTTTGGTTCCAGAGAGGATTTTCTGTTTTATTTAATTTGCTTTTAAAT
    TTTTCTTCTTTGTATAGTCTGTATTTTTAAAAAGGGGTTGAATGAATACCTAAGCAGTTGGAAAATTTGT
    GAGTAGAAACCGAGGGCCTGAGAAACAAATAAATCAAAATTTTGAAATGGCAAAAGAAAGGGTTCATTCT
    ATTTTCTCTTTTCAGAAAAGGTTGCAAAGTTGGATGTGCAAATGGAGATGTTATGGGTGGACTTGGAACA
    TCTTGGGAAGGAAGGAACCCAGGAAAAGAAAGAATGAAATGGCTCAGCCTCAATGACTGAGCAAGAGAGT
    TAGAGTCACCCACAGGAGAAAAGAAGAAAATAAACAGCACTGGGGTGAATTTGGGTCAGAGAAAACCTTA
    AAATGCCAGAGGACAATGTGATGAAAGAGACCATTTGAAAGAAAGGGTTAACCTCATTCCATCAGTCATG
    CCAGAGTTAGAGAATGGGCAGTCTGGAGTTACAAGATCACGATTAGCCTTCCCAGTATGTATACAGCATA
    TTCCTGATAATGAGATGAAGCAAAGTTTGACAAGGTAAGATTGTATCCTATAGTAATATATAGATCAAAG
    GCATGTTGCAGTAAATCTCCAACAGAAATAAGCCCGGGCAATGAAAACACAACTCAATTAATATGAATAC
    GTGCTGTGCACCTAGACTGGGCAGATCTACCACTCCACTACCATATGAGAGATCCCTTATAACTTGTGGT
    TTCTCCAGGCCAGCTGCTTCTGCTCTACTTTCCTTCCTCCTCCTCCTATGTCATCCTCTCCCTCACTCTT
    TCTCTCCCAAAACTTTCAGCTGCACCTTCCCCTCTTCATCCCCCAATCAGTGGCTCTAGCCTTTATTTAT
    AAATTAAGGTAGGAAGAAGGTTTATAGGAAATTACCTGAGTGTTGACGTGTTGATAACCCCACACAAGAG
    AACAAAATTAATATCAAATATGATTAGCTCCAGGGTTATATGCAACAAAGGCATGATAAAGTGGTCATGA
    TTTCATATGAGATGTACTGGACTCATGTGAAAAAACGTTTAAATACATGGGCTTACTCAAAATATAGACT
    CATACCCCCAAGATGGGAAGAGACTGGTAACATTTGTACTGGAGGCTGGTCTGCTGTGGCAGCCATTAAC
    ACAGTGGAGGGAAGAGCCTCAAAGAGTGAAATAGAGGAAGGGGAACAAATACAGTAAACCACCAGTTACT
    ACTTAAAGGGCAGTGTTCTGATGTCCTAGGGAGGGCACTCCAAAATACCAATGGCCTTATCCTTAAATTT
    ATTGTTTAATGAGCCTACACTGATCTCAGTGGCCCACTACTAAGGAGAAATGACAGGCACTTGAACAGCT
    GATATGAGAACAGCTGGAGGCACAGTATACAGAAGAGTTTACAAGCCTATGGAACTCCCCTGTATTTTTT
    TATGATGAGGAAATCTGAAAAATGTAAAATGTTGAAAGATTTAAGAGCAGTTATTAGAGTAGATCAACCA
    ATGAGTCTCTTGCAGCCTGGAATTCTTTTATCTTGTTTGTAACAACAATCAGGCTCCATAAATTATAAAC
    TATAAAAATTTACTTTATAAATTATAAATATATATATATGTGTGTCTGTAGATAGATAGATAGATAAATA
    GATAGATAGATAGATAGATAGATAGATAGATATATGGATTTGATGGTTGCAGCAAATTCTAATTGGTTCC
    AACAACCCTACTCACATAATACATTCATACAGGTTTGATAGATGGAGCTAAGTTTTAAATAAATTATTTT
    TCTCCCATGGCTTATATATACACCACCAAAATTCTCAAAAATTAAAATGTGATTGTGTTGTATTTTTCTT
    TTACTGAATGACTATAAAAAGTTACAACATTCTCCATAAATTTACATGAAAAAATATTATGTAGTGCAGG
    TAAAGAAAACAAATTGACCCAAGAATGGTGTATATTCATTACTAAGCAACTTTTTAGATTCACAAAGTGT
    GGGTAAACAAGGTAATTTTTTCAATCAGTTTTTTTTAACTGGCAGGCAGCAATTCAGAGTTACAATGAGA
    AGATTAATAATTTTATTGTGTATTTTAAAATAAATCTTACAAAAATATTAATAGAATCACAAATTTATAC
    CTTTGTATAAAAACAATCAGTCATTTCTACTTTAAGAAACAGAACTCACATCTACTCATCAATCATTTTA
    TTAAGTCATATTACAAAGCTGAGTGCTAACATGGGTATAAGAAAACCATAACCTTATTCACCAGCCCAGC
    GTCAAAAAGAAAAAAACCAGTCATATCAGCTGCTGCTTCAAGAGTTCTTGTTCCTTGACATTAACAAAAT
    CCCTAGCTTAACTATTAAATTTTTTTTCAAAACTTCTAATTGATCCCTTAGATAAATGTTTGTGCTAACC
    ATCGGGACACATCCCATGAGTTCTGAAGCAGTGTGTTGTTCTTCATGCATGGCCCTTTTGTAGAGCTGTG
    AGTGTAGGGGAAGAGGGGGGAGAGAGAGCCCGTGTCCAGCCAGAGATCCTGTGCTCTGGGCAGGCAGACA
    CGGGAGGACAACGGAACACTTTTCACTCGGCCTTTGGTGGGCATCTGGCTGTGTGAAGTTACTGACCCCA
    CATGGTGGGGGATGGACAAGGGGCAGCCCCTGGTACCAGGAGCCCCAGGGCTACACTCTCGGCCCCAGAT
    ATACAAGAAGAGGGCAGAGGGAGAGAGGCTCCCACACAGGCGAGAGTCCTTAGTCTGGTCTGTGGCTGGA
    GCAGGGGAATTCCTTCTGATTGGAGATTAGGCACAGCTGATTAGGGGAAAGCCTACCCCATCATCCAAGC
    ACAATGGACTTTGAGGAACAGAGCCAGTCTAAGCTTTTATAGCTTTATGGTAGAAAGGCAGGGAGAAAGG
    AGAGAATGTGGAGAGAGACAGAGAGAGACTGGCCATGGCCAAGAGGAGGGAAGGGGGAAGAGAGAGAAAC
    AGGAAAAGCTAGAAAGTAAGATAAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAAAGTG
    GGGCCAAGCAGCCCCTTTTCTAGTGAGCTTGTCATCTCACAGTTGCTAGGTAACTGGGGAGGAGTTTAGT
    CTGAAGGTCAGAAGCTTGGGACCTTGTGTATGTGACTACTAACCACAGCTTCTCCTGTCGGGACTGTGGG
    AGCAGTAACTTTGACAGGAGACAGGGATCCAGGAGACATGAGGGAACACCTTCTGTCCCACATAGGTGGA
    ATCGGTTGTACCAGGGTTCAGAACTCAACTCCACTGGAGACCAGCCTATGTGTGCATAGCCCATTGCCCT
    ACAGCCTTTGACATTCTACAGAGTTCTGAGACTGGTAGAGATACAAAATAGCTAGGATTACACAGAGAAA
    CACTGGCTTGACAATAAAACTTCCAAAACAAATATAAATGAATAAATACACAAATTGGAGCAAGTGAACT
    TGCACTAAATCCATCAAAACTTGCACAGGATGGCTGTCTCCATAATGTTCAGATCTACTTCTATTAATGT
    CATTAAGAAGACAAAGATTATGCACAAAAAGAAACAAAACAAGAAACCCAAAAGGTAAAGGCTCCAATTG
    TAAGTAATAGACCCACAGTCTCTGAGGCCTGACTGTGCAGGTCCTCACCTTTGGTAATCAGCACTGAATG
    CAGACCACTCCTCATGGACTTTGGGCTCAGGTTTGTTTTCCTTGTCCTTATTTTAAAAGGTAATTCATAG
    AAATGAGATCCTGCCAGTATTGTGTACATGAGAAATAGAAAAATTGGTTTTCTTTGCTCTATTTTGTTTT
    GTTTGGTTAATGACAGTTTCCAAATCAGCATTCTTTGCTTTGAGGTGCCCAGTGTGAGGTGAAGCTGGTA
    GGGTCAGGGCAGCCTGGAGGGTCCCTGAAACACTCCTGTGCAGCCTCTGTAGTCACTGTGAGTGACTACT
    GAATGACCTGGGTCCTTCAGGCTCTAAAGAAGGGGCTGGAGAGGGTGGAAATAATTTTTAATGGTGGAGG
    TAGCACCTATTATCCAGACACCATGAAGGGCTGATTCACCATCTACAGAGATGATGCCAGAAACACACTT
    TACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
    TGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTATTCATATATATATATATAGTGTGTATGTATATTCATAT
    ATATATGTATATTCATAAGGTTGAGTAAATGGACTTAAACTGATTCCATCACAACTTCCATGGGATGGAT
    ATTTCCCAGTGTTAAGACCTGTCAACATCACTGTCATTCAGGAGACAATGATTATGCACAACAAAACAAG
    AAACCCAAAAGCAGAGGACTCCAATTACAATAGACCCAGACCCACAGTCTCTGAAGATTGACTGTACAGT
    TCAACCCAGCCCTGTACTTCTCTTCCCTAGAATTTACATTACTGAGTAACTGAGGAAAGCTCTACAATAT
    CTGTTCTCTATAGTGGTCAACACCTCCAAACACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTT
    CTTGCCATAATGTAGACTACTTCAGCCTATTTTGTACTCCAGTTAACGAAACTCAAGACTAGCTGCATGT
    TAGTCCTTATTCTGAAATATTATGAACAGGTGACCTCCCATCATTCACCAATGCAATAATCATATTTAGG
    AATATGAGGTTTTATGAGATATAAGCACAAGGGAGAGAAAGTAAGAAAACTACATAGATATATAGACTGA
    CATAAATCAAGACTTGCATGAGCTAGTGCCCAAGTACCATGTCCCTAAGTGGCAAGGAGTATCTTTTGAG
    CCTAGTGAGATGAGGTACAAATCGGACTCTTACATCTTTTTAGATAAACATGTGAGATCAATGGACTAAA
    GGCGTGAGCTGGGCTTCCTTGCAATCCATTTTCCATACAAGATAACAATAGATCTGGCTCCACAGACACG
    ATGAGAATAGTCTTAATTAGTTCTTTAAGTAGAATGACTGATCACTAAGAGCCCAATTCCATACTAAATA
    CTCTTCTGGATTATGCACAGATAAAAATTGCACATAGGGCATGGGGCACTGATCTCCCTGCACTACATGA
    ATGGGGGCTCATTTACTAAATGTTCCCATGTTTCTTCCTAGTGCTGCACAGAGCAAATCCTACAACTTCC
    TGCTTGTCTACAATGTAAACTCCAGACAGTACCAGAAAATCATTCCTTATGTTCCTCTCCAGGTGCTTCA
    ACAAGCACAGTGCAAATTTCTGTCACCCTG
  • Molecule Role : Vaximmutor
  • Additional Molecule Role : Vaximmutor
  • Related Vaccine(s): S. agalactiae Rib Protein Vaccine
IV. Vaccine Information
1. S. agalactiae BipA Protein Vaccine
a. Vaccine Ontology ID:
VO_0004200
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
Recombinant BipA protein
e. Gene Engineering of BipA
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant: complete Freunds adjuvant
  • VO ID: VO_0000139
  • Description: Complete Freund's adjuvant on day 1, incomplete Freund's adjuvant on day 35 (Santi et al., 2009).
g. Adjuvant: incomplete Freunds adjuvant
  • VO ID: VO_0000142
  • Description: Complete Freund's adjuvant on day 1, incomplete Freund's adjuvant on day 35 (Santi et al., 2009).
h. Immunization Route
Intraperitoneal injection (i.p.)
i. Mouse Response
  • Host Strain: CD‐1
  • Vaccination Protocol: Active mouse maternal immunization.A maternal immunization/neonatal pup challenge model of GBS infection was used to verify the protective efficacy of BibA protein in mice. CD‐1 female mice (6–8 weeks old) were immunized intraperitoneally on days 1 (complete Freund’s adjuvant), 21, and 35 (incomplete Freund’s adjuvant) with either PBS or 20 μg of recombinant protein and were then bred 3 days after the last immunization (Santi et al., 2009).
  • Challenge Protocol: Within 48 h of birth, pups were injected intraperitoneally with a dose of GBS strain 3050 that would be lethal to 90% of the population, corresponding to 3 x 104 colony‐forming units (CFU). Survival of pups was monitored for 3 days after challenge (Santi et al., 2009).
  • Efficacy: Immunization of mice with recombinant BibA conferred protection to 69% of the challenged pups (Santi et al., 2009).
2. S. agalactiae BPS Protein Vaccine
a. Vaccine Ontology ID:
VO_0004050
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
Recombinant BPS
e. Gene Engineering of sar5
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant: aluminum phosphate vaccine adjuvant
  • VO ID: VO_0000128
  • Description: Aluminum phosphate (Adju-Phos; Axell Accurate Chemical & Scientific Corp.) (Erdogan et al., 2002).
g. Immunization Route
subcutaneous injection
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Four-week-old female BALB/c (H-2d) mice were immunized with recombinant BPS protein on days 1, 3, 6, and 27 (30 μg/dose) by the subcutaneous route with aluminum phosphate as the adjuvant (Erdogan et al., 2002).
  • Challenge Protocol: Groups of immunized and control animals were challenged on day 37 with an inoculum of the streptococcal strain Compton R corresponding to the 80% lethal dose (LD80; 5 × 108 bacteria/ml) in nonimmunized mice, and mortality was recorded daily (Erdogan et al., 2002).
  • Efficacy: When the immunized animals were challenged with strain Compton R, approximately 88% of the vaccinated animals survived whereas mice in the control group were not protected (78% lethality) (Erdogan et al., 2002).
3. S. agalactiae Rib Protein Vaccine
a. Vaccine Ontology ID:
VO_0004051
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
Purified Rib protein
e. Gene Engineering of rib
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant: complete Freunds adjuvant
  • VO ID: VO_0000139
  • Description: 0.1 ml of complete Freund’s adjuvant.
g. Immunization Route
subcutaneous injection
h. Mouse Response
  • Host Strain: C3H/HeN
  • Vaccination Protocol: Male C3H/HeN mice, 7 to 13 weeks old, were immunized subcutaneously with 25 μg of pure protein (Rib or bovine serum albumin [BSA]) in 0.1 ml of phosphate-buffered saline (PBS) mixed with 0.1 ml of complete Freund’s adjuvant. Four weeks later the mice were given boosters of the same amount of protein with incomplete Freund’s adjuvant (Larsson et al., 1996).
  • Challenge Protocol: Two weeks after the booster, the mice were injected intraperitoneally (i.p.) with a 90% lethal dose (LD90) of log-phase bacteria, diluted in 0.5 ml of Todd-Hewitt broth. The LD90 for the different strains varied between 106 and 107 bacteria, as determined in preliminary experiments (Larsson et al., 1996).
  • Efficacy: Vaccination of mice with the Rib protein protected against two strains of capsular type III and two strains of type II (Larsson et al., 1996).
4. S. agalactiae Sip Protein Vaccine
a. Vaccine Ontology ID:
VO_0004052
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
Recombinant Sip protein.
e. Gene Engineering of Sip
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant: QuilA vaccine adjuvant
g. Immunization Route
subcutaneous injection
h. Mouse Response
  • Host Strain: CD-1
  • Vaccination Protocol: Mice were injected s.c. three times at 3-week intervals with either 20 μg of purified recombinant Sip protein in 0.1 ml of PBS mixed with 20 μg of QuilA adjuvant (Cedarlane), 15 μg of formaldehyde-killed GBS WC with 20 μg of QuilA as positive controls, or 20 μg of QuilA in PBS as a negative control (Brodeur et al., 2000).
  • Challenge Protocol: To evaluate the challenge dose required for each GBS strain, between 104 and 108 CFU/mouse was injected i.p. into groups of CD-1 female mice (Brodeur et al., 2000).
  • Efficacy: Immunization with the recombinant Sip protein efficiently protected CD-1 mice against deadly challenges with six GBS strains of serotypes Ia/c, Ib, II/R, III, V, and VI (Brodeur et al., 2000).
V. References
1. Brodeur et al., 2000: Brodeur BR, Boyer M, Charlebois I, Hamel J, Couture F, Rioux CR, Martin D. Identification of group B streptococcal Sip protein, which elicits cross-protective immunity. Infection and immunity. 2000; 68(10); 5610-5618. [PubMed: 10992461].
2. Brzychczy-Wloch et al., 2013: Brzychczy-Wloch M, Gorska S, Brzozowska E, Gamian A, Heczko PB, Bulanda M. Identification of high immunoreactive proteins from Streptococcus agalactiae isolates recognized by human serum antibodies. FEMS microbiology letters. 2013; 349(1); 61-70. [PubMed: 24152143].
3. Erdogan et al., 2002: Erdogan S, Fagan PK, Talay SR, Rohde M, Ferrieri P, Flores AE, Guzmán CA, Walker MJ, Chhatwal GS. Molecular analysis of group B protective surface protein, a new cell surface protective antigen of group B streptococci. Infection and immunity. 2002; 70(2); 803-811. [PubMed: 11796614].
4. Grenningloh et al., 2008: Grenningloh R, Darj A, Bauer H, zur Lage S, Chakraborty T, Jacobs T, Weiss S. Liposome-encapsulated antigens induce a protective CTL response against Listeria monocytogenes independent of CD4+ T cell help. Scandinavian journal of immunology. 2008; 67(6); 594-602. [PubMed: 18433404].
5. Kvam et al., 2011: Kvam AI, Mavenyengwa RT, Radtke A, Maeland JA. Streptococcus agalactiae alpha-like protein 1 possesses both cross-reacting and Alp1-specific epitopes. Clinical and vaccine immunology : CVI. 2011; 18(8); 1365-1370. [PubMed: 21653744].
6. Larsson et al., 1996: Larsson C, Stålhammar-Carlemalm M, Lindahl G. Experimental vaccination against group B streptococcus, an encapsulated bacterium, with highly purified preparations of cell surface proteins Rib and alpha. Infection and immunity. 1996; 64(9); 3518-3523. [PubMed: 8751893].
7. Li et al., 2016: Li W, Wang HQ, He RZ, Li YW, Su YL, Li AX. Major surfome and secretome profile of Streptococcus agalactiae from Nile tilapia (Oreochromis niloticus): Insight into vaccine development. Fish & shellfish immunology. 2016; 55; 737-746. [PubMed: 27327442].
8. Liu et al., 2013: Liu G, Zhang W, Lu C. Identification of immunoreactive proteins of Streptococcus agalactiae isolated from cultured tilapia in China. Pathogens and disease. 2013; 69(3); 223-231. [PubMed: 23929656].
9. MicrobeWiki: S. agalactiae: MicrobeWiki: S. agalactiae [http://microbewiki.kenyon.edu/index.php/Streptococcus_agalactiae]
10. Pannaraj et al., 2008: Pannaraj PS, Kelly JK, Rench MA, Madoff LC, Edwards MS, Baker CJ. Alpha C protein-specific immunity in humans with group B streptococcal colonization and invasive disease. Vaccine. 2008; 26(4); 502-508. [PubMed: 18155812].
11. Santi et al., 2007: Santi I, Scarselli M, Mariani M, Pezzicoli A, Masignani V, Taddei A, Grandi G, Telford JL, Soriani M. BibA: a novel immunogenic bacterial adhesin contributing to group B Streptococcus survival in human blood. Molecular microbiology. 2007; 63(3); 754-767. [PubMed: 17212592].
12. Santi et al., 2009: Santi I, Maione D, Galeotti CL, Grandi G, Telford JL, Soriani M. BibA induces opsonizing antibodies conferring in vivo protection against group B Streptococcus. The Journal of infectious diseases. 2009; 200(4); 564-570. [PubMed: 19586417].
13. Santillan et al., 2011: Santillan DA, Rai KK, Santillan MK, Krishnamachari Y, Salem AK, Hunter SK. Efficacy of polymeric encapsulated C5a peptidase-based group B streptococcus vaccines in a murine model. American journal of obstetrics and gynecology. 2011; 205(3); 249.e1-8. [PubMed: 21802065].
14. Suvorov et al., 2010: Suvorov AN, Grabovskaia KB, Leont'eva GF, Meringova LF, Koroleva IN, Duplik NV, Totolian AA. [Recombinant fragments of conservative proteins of group B streptococci as a basis of specific vaccine]. Zhurnal mikrobiologii, epidemiologii, i immunobiologii. 2010; (2); 44-50. [PubMed: 20465000].
15. Wiki: S. agalactiae: Wiki: Streptococcus agalactiae [http://en.wikipedia.org/wiki/Streptococcus_agalactiae]
16. Xue et al., 2010: Xue G, Yu L, Li S, Shen X. Intranasal immunization with GBS surface protein Sip and ScpB induces specific mucosal and systemic immune responses in mice. FEMS immunology and medical microbiology. 2010; 58(2); 202-210. [PubMed: 19912341].
17. Yang et al., 2007: Yang HH, Madoff LC, Guttormsen HK, Liu YD, Paoletti LC. Recombinant group B streptococcus Beta C protein and a variant with the deletion of its immunoglobulin A-binding site are protective mouse maternal vaccines and effective carriers in conjugate vaccines. Infection and immunity. 2007; 75(7); 3455-3461. [PubMed: 17470542].