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Foot-and-mouth disease virus

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Disease
    3. Introduction
  2. Vaccine Related Pathogen Genes
    1. 1D (Protective antigen)
    2. 1D (Protective antigen)
    3. 1D (Protective antigen)
    4. 2A (Protective antigen)
    5. 3C (Protective antigen)
    6. 3D (Protective antigen)
    7. FMDV A12 genome (Protective antigen)
    8. P1 (Protective antigen)
  3. Vaccine Related Host Genes
    1. IL1B
  4. Vaccine Information
    1. FMDV DNA Vaccine encoding P1 and non-structural proteins 2A, 3C, and 3D
    2. Foot and mouth disease DNA vaccine pWRMHX encoding a minimal self-cleaving ribozyme
    3. Foot-and-Mouth Disease Virus Cationic Microparticle-coated DNA Vaccine
    4. Foot-and-Mouth Disease Virus DNA Vaccine pSinCMV-Vac-MEG990
  5. References
I. General Information
1. NCBI Taxonomy ID:
12110
2. Disease:
Foot-and-mouth disease
3. Introduction
Foot-and-mouth disease or hoof-and-mouth disease (Aphtae epizooticae) is an infectious and sometimes fatal viral disease that affects cloven-hoofed animals, including domestic and wild bovids. The virus causes a high fever for two or three days, followed by blisters inside the mouth and on the feet that may rupture and cause lameness. Foot-and-mouth disease is a severe plague for animal farming, since it is highly infectious and can be spread by infected animals through aerosols, through contact with contaminated farming equipment, vehicles, clothing or feed, and by domestic and wild predators. Its containment demands considerable efforts in vaccination, strict monitoring, trade restrictions and quarantines, and occasionally the elimination of millions of animals.
Susceptible animals include cattle, water buffalo, sheep, goats, pigs, antelope, deer, and bison. It has also been known to infect hedgehogs and elephants, and llama and alpaca may develop mild symptoms, but are resistant to the disease and do not pass it on to others of the same species. In laboratory experiments, mice and rats and chickens have been successfully infected by artificial means, but it is not believed that they would contract the disease under natural conditions.Humans are very rarely affected.
The virus responsible for the disease is a picornavirus, the prototypic member of the genus Aphthovirus. Infection occurs when the virus particle is taken into a cell of the host. The cell is then forced to manufacture thousands of copies of the virus, and eventually bursts, releasing the new particles in the blood. The virus is highly variable,which limits the effectiveness of vaccination (Wiki: Foot and mouth disease).
1. 1D
  • Gene Name : 1D
  • Sequence Strain (Species/Organism) : Foot-and-mouth disease virus - type Asia 1
  • NCBI Protein GI : 148800633
  • Other Database IDs : CDD:301302
    CDD:119412
  • Taxonomy ID : 110195
  • Gene Strand (Orientation) : ?
  • Protein Name : 1D protein
  • Protein pI : 9.49
  • Protein Weight : 22686.12
  • Protein Length : 293
  • Protein Note : Picornavirus capsid protein domain_like. Picornaviruses are non-enveloped plus-strand ssRNA animal viruses with icosahedral capsids composed of 60 copies each of 4 virus encoded proteins; alignment includes picornaviridae, like poliovirus, hepatitis A...; cl13999
  • Protein Sequence : Show Sequence
    >ABR13034.1 1D protein, partial [Foot-and-mouth disease virus - type Asia 1]
    TTTTGESADPVTTTVENYGGETQTARRLHTDVAFVLDRFVKLTAPKNIQTLDLMQIPSHTLVGALLRSAT
    YYFSDLEVAIVHTGPITWVPNGSPKDALDNQTNPTAYQKQPVTRLALPYTAPHRVLATVYNGKTTYGETT
    ARRGDMAALAQRLSGRLPTSFNYGAVKAETITELLFRMKRAETYCPRPLLALDTTQDRRKQEIIAPEKQM
    M
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): Foot-and-Mouth Disease Virus Cationic Microparticle-coated DNA Vaccine , Foot-and-Mouth Disease Virus DNA Vaccine pSinCMV-Vac-MEG990
2. 1D
  • Gene Name : 1D
  • Sequence Strain (Species/Organism) : Foot-and-mouth disease virus - type O
  • NCBI Protein GI : 56675803
  • Other Database IDs : GOA:Q9DJ81
    InterPro: IPR004080
    UniProtKB/TrEMBL: Q9DJ81
  • Taxonomy ID : 12118
  • Gene Strand (Orientation) : ?
  • Protein Name : VP1
  • Protein pI : 10.73
  • Protein Weight : 6939.818
  • Protein Length : 126
  • Protein Sequence : Show Sequence
    >CAC22182.2 VP1, partial [Foot-and-mouth disease virus - type O]
    KAERALPTSFNYGAIKATRVTELLYRXKRAEXXXPRPXXAXHPSEARHKQKIVAPVXQX
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): Foot-and-Mouth Disease Virus DNA Vaccine pSinCMV-Vac-MEG990
3. 1D
  • Gene Name : 1D
  • Sequence Strain (Species/Organism) : Foot-and-mouth disease virus - type A
  • NCBI Protein GI : 13399192
  • Other Database IDs : CDD:301302
    CDD:119412
    GOA:Q98VZ6
    InterPro: IPR001676
    InterPro: IPR004080
    UniProtKB/TrEMBL: Q98VZ6
  • Taxonomy ID : 12111
  • Gene Strand (Orientation) : ?
  • Protein Name : VP1
  • Protein pI : 9.7
  • Protein Weight : 22399.68
  • Protein Length : 283
  • Protein Note : Picornavirus capsid protein domain_like. Picornaviruses are non-enveloped plus-strand ssRNA animal viruses with icosahedral capsids composed of 60 copies each of 4 virus encoded proteins; alignment includes picornaviridae, like poliovirus, hepatitis A...; cl13999
  • Protein Sequence : Show Sequence
    >CAC34727.1 VP1, partial [Foot-and-mouth disease virus - type A]
    TTATGESADPVTTTVENYGGETQVQRRHHTDIGFIMDRFVRIKNVSQTHVIDLMQTHQHGLVGALLRAAT
    YYFSDLEIVVKHDGNLTWVPNGAPESALSNTSNPTVYNKAPFTRLALPYTAPHRVLATVYNGTSKYAVGG
    SARRGDMGSLAARVARQLPSSFNYGAIKAENIHELLVRMKRAELYCPRPLLAIEVSSQDRHKQKIIAPAK
    QLL
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): Foot-and-Mouth Disease Virus DNA Vaccine pSinCMV-Vac-MEG990
4. 2A
  • Gene Name : 2A
  • Sequence Strain (Species/Organism) : Foot-and-mouth disease virus - type O
  • NCBI Protein GI : 25121634
  • Taxonomy ID : 12118
  • Gene Strand (Orientation) : ?
  • Protein Name : 2A
  • Protein pI : 3.88
  • Protein Weight : 2245.32
  • Protein Length : 76
  • Protein Note : isolated from vesicular fluid of animals inoculated with field derived material in April 1997
  • Protein Sequence : Show Sequence
    >NP_740461.1 2A [Foot-and-mouth disease virus - type O]
    LLNFDLLKLAGDVESNPG
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): FMDV DNA Vaccine encoding P1 and non-structural proteins 2A, 3C, and 3D
5. 3C
  • Gene Name : 3C
  • Sequence Strain (Species/Organism) : Foot-and-mouth disease virus - type O
  • NCBI Protein GI : 25121639
  • Other Database IDs : CDD:201298
  • Taxonomy ID : 12118
  • Gene Strand (Orientation) : ?
  • Protein Name : 3C
  • Protein pI : 8.06
  • Protein Weight : 21623.2
  • Protein Length : 274
  • Protein Note : isolated from vesicular fluid of animals inoculated with field derived material in April 1997
  • Protein Sequence : Show Sequence
    >NP_740466.1 3C [Foot-and-mouth disease virus - type O]
    SGAPPTDLQKMVMGNTKPVELILDGKTVAICCATGVFGTAYLVPRHLFAEKYDKIMLDGRALTDSDYRVF
    EFEIKVKGQDMLSDAALMVLHRGNRVRDITKHFRDVARMKKGTPVVGVINNADVGRLIFSGEALTYKDIV
    VCMDGDTMPGLFAYRASTKAGYCGGAVLAKDGAETFIVGTHSAGGNGIGYCSCVSRSMLLKMKAHIDPEP
    HHE
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): FMDV DNA Vaccine encoding P1 and non-structural proteins 2A, 3C, and 3D
6. 3D
  • Gene Name : 3D
  • Sequence Strain (Species/Organism) : Foot-and-mouth disease virus - type O
  • NCBI Protein GI : 25121640
  • Other Database IDs : CDD:73158
  • Taxonomy ID : 12118
  • Gene Strand (Orientation) : ?
  • Protein Name : 3D
  • Protein pI : 6.09
  • Protein Weight : 49457.15
  • Protein Length : 534
  • Protein Note : isolated from vesicular fluid of animals inoculated with field derived material in April 1997
  • Protein Sequence : Show Sequence
    >NP_740467.1 3D [Foot-and-mouth disease virus - type O]
    GLIVDTRDVEERVHVMRKTKLAPTVAHGVFNPEFGPAALSNKDPRLNEGVVLDDVIFSKHKGDTRMSEED
    KALFRRCAADYASRLHSVLGTANAPLSVYEAIKGVDGLDAMEPDTAPGLPWALQGKRRGALIDFENGTVG
    PEVEAALKLMESREYKFVCQTFLKDEIRPLEKVRAGKTRIVDVLPVEHILYTRMMIGRFCAQMHSNNGPQ
    IGSAVGCNPDVDWQRFGTHFAQYKNVWDVDYSAFDANHCSDAMNIMFEEVFRTEFGFHPNAEWILKTLVN
    TEHAYENKRIVVEGGMPSGCSATSIINTILNNIYVLYALRRHYEGVELDTYTMISYGDDIVVASDYDLDF
    EALKPHFKSLGQTITPADKSDKGFVLGHSITDVTFLKRHFHMDYGTGFYKPVMASKTLEAILSFARRGTI
    QEKLISVAGLAVHSGPDEYRRLFEPFQGLFEIPSYRSLYLRWVNAVCGDA
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): FMDV DNA Vaccine encoding P1 and non-structural proteins 2A, 3C, and 3D
7. FMDV A12 genome
  • Gene Name : FMDV A12 genome
  • Sequence Strain (Species/Organism) : Foot-and-mouth disease virus - type A strain A12-119
  • NCBI Nucleotide GI : 46810760
  • NCBI Protein GI : 46810761
  • Other Database IDs : CDD:283147
    CDD:286076
    CDD:278501
    CDD:119412
    CDD:301302
    CDD:279280
    CDD:278947
    CDD:279070
    CDD:238843
  • Taxonomy ID : 12111
  • Gene Strand (Orientation) : ?
  • Protein Name : polyprotein
  • Protein pI : 6.78
  • Protein Weight : 242060.45
  • Protein Length : 2432
  • Protein Note : Plum Island Animal Disease Center Virus Collection, isolated 1932, vaccine
  • Protein Sequence : Show Sequence
    >AAT01695.1 polyprotein [Foot-and-mouth disease virus - type A]
    MNTTNCFIALVHAIREIRAFFLSRATGKMEFTLYNGERKTFYSRPNNHDNCWLNTILQLFRYVDEPFFDW
    VYNSPENLTLAAIKQLEELTGLELHEGGPPALVIWNIKHLLQTGIGTASRPSEVCMVDGTDMCLADFHAG
    IFLKGQEHAVFACVTSNGWYAIDDEDFYPWTPDPSDVLVFVPYDQEPLNGGWKANVQRKLKGAGQSSPAT
    GSQNQSGNTGSIINNYYMQQYQNSMDTQLGDNAISGGSNEGSTDTTSTHTTNTQNNDWFSKLASSAFTGL
    FGALLADKKTEETTLLEDRILTTRNGHTTSTTQSSVGVTYGYSTEEDHVAGPNTSGLETRVVQAERFFKK
    FLFDWTTDKPFGHLEKLELPTDHHGVFGHLVDSYAYMRNGWDVEVSAVGNQFNGGCLLVAMVPEWKEFDK
    REKYQLTLFPHQFISPRTNMTAHITVPYLGVNRYDQYKKHKPWTLVIMVVSPLTVSNTAATQIKVYANIA
    PTYVHVAGELPSKEGIFPVACSDGYGGLVTTDPKTADPVYGKVYNPPRTNYPGRFTNLLDVAEACPTFLC
    FDDGKPYVVTRTDDTRLLAKFDVSLAAKHMSNTYLSGIAQYYTQYSGTINLHFMFTGSTDSKARYMVAYI
    PPGVETPPETPEGAAHCIHAEWDTGLNSKFTFSIPYVSAADYAYTASDTAETTNVQGWVCIYQITHGKAE
    GDTLVVSASAGKDFELRLPIDPRSQTTATGESADPVTTTVENYGGETQVQRRHHTDVSFIMDRFVKIKSL
    NPTHVIDLMQTHQHGLVGALLRAATYYFSDLEIVVRHDGNLTWVPNGAPEAALSNTGNPTAYNKAPFTRL
    ALPYTAPHRVLATVYNGTNKYSASGSGVRGDSGSLAPRVARQLPASFNYGAIKAETIHELLVRMKRAELY
    CPRPLLAIEVSSQDRHKQKIIAPGKQLLNFDLLKLAGDVESNPGPFFFADVRSNFSKLVDTINQMQEDMS
    TKHGPDFNRLVSAFEELATGVKAIRTGLDEAKPWYKLIKLLSRLSCMAAVAARSKDPVLVAIMLADTGLE
    ILDSTFVVKKISDSLSSLFHVPAPVFSFGAPVLLAGLVKVASSFFRSTPEDLERAEKQLKARDINDIFAI
    LKNGEWLVKLILAIRDWIKAWIASEEKFVTTTDLVPGILEKQRDLNDPSKYKEAKEWLDNARQACLKSGN
    VHIANLCKVVAPAPSKSRPEPVVVCLRGKSGQGKSFLANVLAQAISTHFTGRTDSVWYCPPDPDHFDGYN
    QQTVVVMDDLGQNPDGKDFKYFAQMVSTTGFIPPMASLEDKGKPFNSKVIIATTNLYSGFTPRTMVCPDA
    LNRRFHFDIDVSAKDGYKINNKLDIIKALEDTHTNPVAMFQYDCALLNGMAVEMKRMQQDMFKPQPPLQN
    VYQLVQEVIERVELHEKVSSHPIFKQISIPSQKSVLYFLIEKGQHEAAIEFFEGMVHDSIKEELRPLIQQ
    TSFVKRAFKRLKENFEIVALCLTLLANIVIMIRETRKRQKMVDDAVNEYIEKANITTDDTTLDEAEKNPL
    ETSGASTVGFRERTLTGQRACNDVNSEPARPAEEQPQAEGPYTGPLERQRPLKVRAKLPQQEGPYAGPLE
    RQKPLKVKAKAPVVKEGPYEGPVKKPVALKVKAKNLIVTESGAPPTDLQKMVMGNTKPVELILDGKTVAI
    CCATGVFGTAYLVPRHLFAEKYDKIMLDGRAMTDSDYRVFEFEIKVKGQDMLSDAALMVLHRGNRVRDIT
    KHFRDTARMKKGTPVVGVVNNADVGRLIFSGEALTYKDIVVCMDGDTMPGLFAYKAATKAGYCGGAVLAK
    DGADTFIVGTHSAGGNGVGYCSCVSRSMLLRMKAHVDPEPHHEGLIVDTRDVEERVHVMRKTKLAPTVAH
    GVFNPEFGPAALSNKDPRLNEGVVLDEVIFSKHKGDTKMSAEDKALFRRCAADYASRLHSVLGTANAPLS
    IYEAIKGVDGLDAMESDTAPGLPWAFQGKRRGALIDFENGTVGPEVEAALKLMEKREYKFACQTFLKDEI
    RPMEKVRAGKTRIVDVLPVEHILYTRMMIGRFCAQMHSNNGPQIGSAVGCNPDVDWQRFGTHFAQYRNVW
    DVDYSAFDANHCSDAMNIMFEEVFRTDFGFHPNAEWILKTLVNTEHAYENKRITVEGGMPSGCSATSIIN
    TILNNIYVLYALRRHYEGVELDTYTMISYGDDIVVASDYDLDFEALKPHFKSLGQTITPADKSDKGFVLG
    HSITDVTFLKRHFHIDYGTGFYKPVMASKTLEAILSFARRGTIQEKLTSVAGLAVHSGPDEYRRLFEPFQ
    GLFEIPSYRSLYLRWVNAVCGDA
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): Foot and mouth disease DNA vaccine pWRMHX encoding a minimal self-cleaving ribozyme
8. P1
  • Gene Name : P1
  • Sequence Strain (Species/Organism) : Foot-and-mouth disease virus - type O
  • NCBI Protein GI : 15419537
  • Other Database IDs : CDD:286076
    CDD:278501
    CDD:119412
    CDD:301302
  • Taxonomy ID : 12118
  • Gene Strand (Orientation) : ?
  • Protein Name : polyprotein P1
  • Protein pI : 6.71
  • Protein Weight : 75741.482
  • Protein Length : 824
  • Protein Note : isolated from a field outbreak in 1979
  • Protein Sequence : Show Sequence
    >AAK97050.1 polyprotein P1, partial [Foot-and-mouth disease virus - type O]
    GAGQSSPATGSQNQSGNTGSIINNYYMQQYQNSMDTQLGDNAISGGSNEGSTDTTSTHTTNTQNNDWFSK
    LASSAFSGLFGALLADKKTEETTLLEDRILTTRNGHTTSTTQSSVGVTYGYATAEDFVRGPNTSGLETRV
    IQAERFFKTHLFDWVTSDSFGRCHLLELPTDHKGVYGSLTDSYAYMRNGWDVEVTAVGNQFNGGCLLVAM
    VPELCSIQKRELYQLTLFPHQFINPRTNLTAHITVPFIGVNRYDQYXVHKPWTLVVMVVAPLTVNTEGAP
    QIKVYANIAPTNVHVAGEFPSKEGIFPVACSDGYGGLVTTDPKTADPAYGKVFNPPRNMLPGRFTNFLDV
    AEACPTFLRFEGGVPYVTSKTESDRVLTRFDLSLAAKHMSNTFLAGLAQYYTQYSGTINLHFMFTGPTDA
    KARYMIAYAPPGMEPPKTPEAAAHCIHAEWDTGLNSKLTFSIPYLSAADYAYTASDTAETTNVQGWVCLF
    QITHGKADGDALVVLASAGKDFEVRLPVDARTQTTSAGESADPVTATVENYGGETQVQRRQHTDVSFILD
    RFVKVTPEDQINVLDLMQTPAHTLVGALLRTATYYFADLEVAVKHEGNLTWVPNGAPETALDNTTNPTAY
    HKAPLTRLALPYTAPHRVLATVYNGNCRYGESTVTNVRGDLQVLAQKAARTLPTSFNYGAIKATRVIELL
    YRMKRAETYCPRPLLAIHPNEARHKQKIVAPVKQLL
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): FMDV DNA Vaccine encoding P1 and non-structural proteins 2A, 3C, and 3D
1. IL1B
  • Gene Name : IL1B
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 3553
  • NCBI Protein GI : 10835145
  • Genbank Accession : AC079753
  • Protein Accession : NP_000567
  • Taxonomy ID : 9606
  • Chromosome No : 2
  • Gene Starting Position : 5000
  • Gene Ending Position : 12019
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 1, beta
  • Protein pI : 4.45
  • Protein Weight : 29060.74
  • Protein Length : 269
  • Protein Note : Also known as IL-1; IL1F2; IL1-BETA
  • DNA Sequence : Show Sequence
    >gi|210032106:5000-12019 Homo sapiens interleukin 1, beta (IL1B), RefSeqGene on chromosome 2
    TACCAAACCTCTTCGAGGCACAAGGCACAACAGGCTGCTCTGGGATTCTCTTCAGCCAATCTTCATTGCT
    CAAGTATGACTTTAATCTTCCTTACAACTAGGTGCTAAGGGAGTCTCTCTGTCTCTCTGCCTCTTTGTGT
    GTATGCATATTCTCTCTCTCTCTCTCTTTCTTTCTCTGTCTCTCCCTCTCCTTCCCTCTCTGCCTCCCTC
    TCTCAGCTTTTTGCAAAAATGCCAGGTGTAATATAATGCTTATGACTCGGGAAATATTCTGGGAATGGAT
    ACTGCTTATCTAACAGCTGACACCCTAAAGGTTAGTGTCAAAGCCTCTGCTCCAGCTCTCCTAGCCAATA
    CATTGCTAGTTGGGGTTTGGTTTAGCAAATGCTTTTCTCTAGACCCAAAGGACTTCTCTTTCACACATTC
    ATTCATTTACTCAGAGATCATTTCTTTGCATGACTGCCATGCACTGGATGCTGAGAGAAATCACACATGA
    ACGTAGCCGTCATGGGGAAGTCACTCATTTTCTCCTTTTTACACAGGTGTCTGAAGCAGCCATGGCAGAA
    GTACCTGAGCTCGCCAGTGAAATGATGGCTTATTACAGGTCAGTGGAGACGCTGAGACCAGTAACATGAG
    CAGGTCTCCTCTTTCAAGAGTAGAGTGTTATCTGTGCTTGGAGACCAGATTTTTCCCCTAAATTGCCTCT
    TTCAGTGGCAAACAGGGTGCCAAGTAAATCTGATTTAAAGACTACTTTCCCATTACAAGTCCCTCCAGCC
    TTGGGACCTGGAGGCTATCCAGATGTGTTGTTGCAAGGGCTTCCTGCAGAGGCAAATGGGGAGAAAAGAC
    TCCAAGCCCACAATACAAGGAATCCCTTTGCAAAGTGTGGCTTGGAGGGAGAGGGAGAGCTCAGATTTTA
    GCTGACTCTGCTGGGCTAGAGGTTAGGCCTCAAGATCCAACAGGGAGCACCCAGGGTGCCCACCTGCCAG
    GCCTAGAATCTGCCTTCTGGACTGTTCTGCGCATATCACTGTGAAACTTGCCAGGTGTTTCAGGCAGCTT
    TGAGAGGCAGGCTGTTTGCAGTTTCTTATGAACAGTCAAGTCTTGTACACAGGGAAGGAAAAATAAACCT
    GTTTAGAAGACATAATTGAGACATGTCCCTGTTTTTATTACAGTGGCAATGAGGATGACTTGTTCTTTGA
    AGCTGATGGCCCTAAACAGATGAAGGTAAGACTATGGGTTTAACTCCCAACCCAAGGAAGGGCTCTAACA
    CAGGGAAAGCTCAAAGAAGGGAGTTCTGGGCCACTTTGATGCCATGGTATTTTGTTTTAGAAAGACTTTA
    ACCTCTTCCAGTGAGACACAGGCTGCACCACTTGCTGACCTGGCCACTTGGTCATCATATCACCACAGTC
    ACTCACTAACGTTGGTGGTGGTGGCCACACTTGGTGGTGACAGGGGAGGAGTAGTGATAATGTTTCCCAT
    TTCATAGTAGGAAGACAACCAAGTCTTCAACATAAATTTGATTATCCTTTTAAGAGATGGATTCAGCCTA
    TGCCAATCACTTGAGTTAAACTCTGAAACCAAGAGATGATCTTGAGAACTAACATATGTCTACCCCTTTT
    GAGTAGAATAGTTTTTTGCTACCTGGGGTGAAGCTTATAACAACAAGACATAGATGATATAAACAAAAAG
    ATGAATTGAGACTTGAAAGAAAACCATTCACTTGCTGTTTGACCTTGACAAGTCATTTTACCCGCTTTGG
    ACCTCATCTGAAAAATAAAGGGCTGAGCTGGATGATCTCTGAGATTCCAGCATCCTGCAACCTCCAGTTC
    TGAAATATTTTCAGTTGTAGCTAAGGGCATTTGGGCAGCAAATGGTCATTTTTCAGACTCATCCTTACAA
    AGAGCCATGTTATATTCCTGCTGTCCCTTCTGTTTTATATGATGCTCAGTAGCCTTCCTAGGTGGCCCAG
    CCATCAGCCTAGCTAGGTCAGTTGTGCAGGTTGGGAGGCAGCCACTTTTCTCTGGCTTTATTTTATTCCA
    GTTTGTGATAGCCTCCCCTAGCCTCATAATCCAGTCCTCAATCTTGTTAAAAACATATTTCTTTAGAAGT
    TTTAAGACTGGCATAACTTGTTGGCTGCAGCTGTGGGAGGAGCCCATTGGCTTGTCTGCCTGGCCTTTGC
    CCCCATTGCCTCTTCCAGCAGCTTGGCTCTGCTCCAGGCAGGAAATTCTCTCCTGCTCAACTTTCTTTTG
    TGCACTTACAGGTCTCTTTAACTGTCTTTCAAGCCTTTGAACCATTATCATGCCTTAAGGCAACCTCAGT
    GAAGCCTTAATACGGAGCTTCTCTGAATAAGAGGAAAGTGGTAACATTTCACAAAAAGTACTCTCACAGG
    ATTTGCAGAATGCCTATGAGACAGTGTTATGAAAAAGGAAAAAAAAGAACAGTGTAGAAAAATTGAATAC
    TTGCTGAGTGAGCATAGGTGAATGGAAAATGTTATGGTCATCTGCATGAAAAAGCAAATCATAGTGTGAC
    AGCATTAGGGATACAAAAAGATATAGAGAAGGTATACATGTATGGTGTAGGTGGGGCATGTACAAAAAAG
    ATGAACAAAGTAGAAATGGGATTTATTCTAAAAGAATAGCCTGTAAGGTGTCAGAAAGCCCACATTCTAG
    TCTTGAGTCTGCCTCTAACCTGCTGTGTGCCCTTGAGTACACACTTAACCTCCTTGAGCTTCAGAGAGGG
    ATAATCTTTTTATTTTATTTTATTTTATTTTGTTTTGTTTTGTTTTGTTTTGTTTTATGAGACAGAGTCT
    CACTCTGTTGCCCAGGCTGGAGTGCAGTGGTACAATCTTGGCTTACTGCATCCTCCACCTCCTGAGTTCA
    AGCGATTCTCCTTCCTCAGTCTCCTGAATAGCTAGGATTACAGGTGCACCCCACCACACCCAGCTAATTT
    TTGTATTTTTAGTAGAGAAGGGGTTTCGCCATGTTGGCCAGGCTGGTTTTGAAGTCCTGACCTAAATGAT
    TCATCCACCTCGGCTTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCACGCCTGGCCCAGAGAGGGAT
    GATCTTTAGAAGCTCGGGATTCTTTCAAGCCCTTTCCTCCTCTCTGAGCTTTCTACTCTCTGATGTCAAA
    GCATGGTTCCTGGCAGGACCACCTCACCAGGCTCCCTCCCTCGCTCTCTCCGCAGTGCTCCTTCCAGGAC
    CTGGACCTCTGCCCTCTGGATGGCGGCATCCAGCTACGAATCTCCGACCACCACTACAGCAAGGGCTTCA
    GGCAGGCCGCGTCAGTTGTTGTGGCCATGGACAAGCTGAGGAAGATGCTGGTTCCCTGCCCACAGACCTT
    CCAGGAGAATGACCTGAGCACCTTCTTTCCCTTCATCTTTGAAGAAGGTAGTTAGCCAAGAGCAGGCAGT
    AGATCTCCACTTGTGTCCTCTTGGAAGTCATCAAGCCCCAGCCAACTCAATTCCCCCAGAGCCAAAGCCC
    TTTAAAGGTAGAAGGCCCAGCGGGGAGACAAAACAAAGAAGGCTGGAAACCAAAGCAATCATCTCTTTAG
    TGGAAACTATTCTTAAAGAAGATCTTGATGGCTACTGACATTTGCAACTCCCTCACTCTTTCTCAGGGGC
    CTTTCACTTACATTGTCACCAGAGGTTCGTAACCTCCCTGTGGGCTAGTGTTATGACCATCACCATTTTA
    CCTAAGTAGCTCTGTTGCTCGGCCACAGTGAGCAGTAATAGACCTGAAGCTGGAACCCATGTCTAATAGT
    GTCAGGTCCAGTGTTCTTAGCCACCCCACTCCCAGCTTCATCCCTACTGGTGTTGTCATCAGACTTTGAC
    CGTATATGCTCAGGTGTCCTCCAAGAAATCAAATTTTGCCGCCTCGCCTCACGAGGCCTGCCCTTCTGAT
    TTTATACCTAAACAACATGTGCTCCACATTTCAGAACCTATCTTCTTCGACACATGGGATAACGAGGCTT
    ATGTGCACGATGCACCTGTACGATCACTGAACTGCACGCTCCGGGACTCACAGCAAAAAAGCTTGGTGAT
    GTCTGGTCCATATGAACTGAAAGCTCTCCACCTCCAGGGACAGGATATGGAGCAACAAGGTAAATGGAAA
    CATCCTGGTTTCCCTGCCTGGCCTCCTGGCAGCTTGCTAATTCTCCATGTTTTAAACAAAGTAGAAAGTT
    AATTTAAGGCAAATGATCAACACAAGTGAAAAAAAATATTAAAAAGGAATATACAAACTTTGGTCCTAGA
    AATGGCACATTTGATTGCACTGGCCAGTGCATTTGTTAACAGGAGTGTGACCCTGAGAAATTAGACGGCT
    CAAGCACTCCCAGGACCATGTCCACCCAAGTCTCTTGGGCATAGTGCAATGTCAATTCTTCCACAATATG
    GGGTCATTTGATGGACATGGCCTAACTGCCTGTGGGTTCTCTCTTCCTGTTGTTGAGGCTGAAACAAGAG
    TGCTGGAGCGATAATGTGTCCATCCCCCTCCCCAGTCTTCCCCCCTTGCCCCAACATCCGTCCCACCCAA
    TGCCAGGTGGTTCCTTGTAGGGAAATTTTACCGCCCAGCAGGAACTTATATCTCTCCGCTGTAACGGGCA
    AAAGTTTCAAGTGCGGTGAACCCATCATTAGCTGTGGTGATCTGCCTGGCATCGTGCCACAGTAGCCAAA
    GCCTCTGCACAGGAGTGTGGGCAACTAAGGCTGCTGACTTTGAAGGACAGCCTCACTCAGGGGGAAGCTA
    TTTGCTCTCAGCCAGGCCAAGAAAATCCTGTTTCTTTGGAATCGGGTAGTAAGAGTGATCCCAGGGCCTC
    CAATTGACACTGCTGTGACTGAGGAAGATCAAAATGAGTGTCTCTCTTTGGAGCCACTTTCCCAGCTCAG
    CCTCTCCTCTCCCAGTTTCTTCCCATGGGCTACTCTCTGTTCCTGAAACAGTTCTGGTGCCTGATTTCTG
    GCAGAAGTACAGCTTCACCTCTTTCCTTTCCTTCCACATTGATCAAGTTGTTCCGCTCCTGTGGATGGGC
    ACATTGCCAGCCAGTGACACAATGGCTTCCTTCCTTCCTTCCTTCAGCATTTAAAATGTAGACCCTCTTT
    CATTCTCCGTTCCTACTGCTATGAGGCTCTGAGAAACCCTCAGGCCTTTGAGGGGAAACCCTAAATCAAC
    AAAATGACCCTGCTATTGTCTGTGAGAAGTCAAGTTATCCTGTGTCTTAGGCCAAGGAACCTCACTGTGG
    GTTCCCACAGAGGCTACCAAATTACATGTATCCTACTCATGGGGCCTAGGGGTTGGGGTGACCCTGCACT
    GCTGTGTCCCTAACCACAAGACCCCCTTCTTTCTTCAGTGGTGTTCTCCATGTCCTTTGTACAAGGAGAA
    GAAAGTAATGACAAAATACCTGTGGCCTTGGGCCTCAAGGAAAAGAATCTGTACCTGTCCTGCGTGTTGA
    AAGATGATAAGCCCACTCTACAGCTGGAGGTAAGTGAATGCTATGGAATGAAGCCCTTCTCAGCCTCCTG
    CTACCACTTATTCCCAGACAACCACCTTCTCCCCGCCCCCATCCCTAGGAAAAGCTGGGAACAGGTCTAT
    TTGACAATTTTGCATTAATGTAAATAAATTTAACATAATTTTTAACTGCGTGCAACCTTCAATCCTGCTG
    CAGAAAATTAAATCATTTTGCCGATGTTATTATGTCCTACCATAGTTACAACCCCAACAGATTATATATT
    GTTAGGGCTGCTCTCATTTGATAGACACCTTGGGAAATAGATGACTTAAAGGGTCCCATTATCATGTCCA
    CTCCACTCCCAAAATTACCACCACTATCACCTCCAGCTTTCTCAGCAAAAGCTTCATTTCCAAGTTGATG
    TCATTCTAGGACCATAAGGAAAAATACAATAAAAAGCCCCTGGAAACTAGGTACTTCAAGAAGCTCTAGC
    TTAATTTTCACCCCCCCAAAAAAAAAAAATTCTCACCTACATTATGCTCCTCAGCATTTGGCACTAAGTT
    TTAGAAAAGAAGAAGGGCTCTTTTAATAATCACACGGAAAGTTGGGGGCCCAGTTACAACTCAGGAGTCT
    GGCTCCTGATCATGTGACCTGCTCGTCAGTTTCCTTTCTGGCCAACCCAAAGAACATCTTTCCCATAGCA
    TCTTTGTCCCTTGCCCCACAAAAATTCTTCTTTCTCTTTCGCTGCAGAGTGTAGATCCCAAAAATTACCC
    AAAGAAGAAGATGGAAAAGCGATTTGTCTTCAACAAGATAGAAATCAATAACAAGCTGGAATTTGAGTCT
    GCCCAGTTCCCCAACTGGTACATCAGCACCTCTCAAGCAGAAAACATGCCCGTCTTCCTGGGAGGGACCA
    AAGGCGGCCAGGATATAACTGACTTCACCATGCAATTTGTGTCTTCCTAAAGAGAGCTGTACCCAGAGAG
    TCCTGTGCTGAATGTGGACTCAATCCCTAGGGCTGGCAGAAAGGGAACAGAAAGGTTTTTGAGTACGGCT
    ATAGCCTGGACTTTCCTGTTGTCTACACCAATGCCCAACTGCCTGCCTTAGGGTAGTGCTAAGAGGATCT
    CCTGTCCATCAGCCAGGACAGTCAGCTCTCTCCTTTCAGGGCCAATCCCCAGCCCTTTTGTTGAGCCAGG
    CCTCTCTCACCTCTCCTACTCACTTAAAGCCCGCCTGACAGAAACCACGGCCACATTTGGTTCTAAGAAA
    CCCTCTGTCATTCGCTCCCACATTCTGATGAGCAACCGCTTCCCTATTTATTTATTTATTTGTTTGTTTG
    TTTTATTCATTGGTCTAATTTATTCAAAGGGGGCAAGAAGTAGCAGTGTCTGTAAAAGAGCCTAGTTTTT
    AATAGCTATGGAATCAATTCAATTTGGACTGGTGTGCTCTCTTTAAATCAAGTCCTTTAATTAAGACTGA
    AAATATATAAGCTCAGATTATTTAAATGGGAATATTTATAAATGAGCAAATATCATACTGTTCAATGGTT
    CTGAAATAAACTTCACTGAA
  • Protein Sequence : Show Sequence
    >gi|10835145|ref|NP_000567.1| interleukin-1 beta proprotein [Homo sapiens]
    MAEVPELASEMMAYYSGNEDDLFFEADGPKQMKCSFQDLDLCPLDGGIQLRISDHHYSKGFRQAASVVVA
    MDKLRKMLVPCPQTFQENDLSTFFPFIFEEEPIFFDTWDNEAYVHDAPVRSLNCTLRDSQQKSLVMSGPY
    ELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKK
    MEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS
  • Molecule Role : Vaximmutor
IV. Vaccine Information
1. FMDV DNA Vaccine encoding P1 and non-structural proteins 2A, 3C, and 3D
a. Vaccine Ontology ID:
VO_0004553
b. Type:
DNA vaccine
c. Status:
Research
d. Gene Engineering of P1
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
e. Gene Engineering of 2A
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
f. Gene Engineering of 3C
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
g. Gene Engineering of 3D
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
h. Vector:
pcDNA3.1/P1-2A3C3D (Fowler et al., 2012)
i. Immunization Route
Intramuscular injection (i.m.)
j. Cattle Response
  • Vaccination Protocol: Thirty-two cattle of 6–7 months of age were housed separately in six groups of five (vaccinated animals) and one group of two (un-vaccinated controls) within the isolation units at the Institute for Animal Health, Pirbright. The six groups of vaccinates were immunised as follows: Group 1 with 2 mg PLG-pcDNA3.1/P12A3C3D + 0.4 mg pcDNA3.1/bGM-CSF (DNA + PLG + GMCSF), Group 2 with 2 mg PLG-pcDNA3.1/P12A3C3D + 0.4 mg pcDNA3.1/bGM-CSF + electroporation (DNA + PLG + GMCSF + elec), Group 3 with 2 mg pcDNA3.1/P12A3C3D + 0.4 mg pcDNA3.1/bGM-CSF (DNA + GMCSF), Group 4 with 2 mg pcDNA3.1/P12A3C3D + 0.4 mg pcDNA3.1/bGM-CSF + electroporation (DNA + GMCSF + elec), Group 5 with 2 mg pcDNA3.1/P12A3C3D + electroporation (DNA + elec), and Group 6 with conventional vaccine (Fowler et al., 2012).
  • Vaccine Immune Response Type: VO_0003057
  • Immune Response: All DNA vaccinated cattle generated a detectable neutralising antibody response at 21 days post 1st vaccination (Fowler et al., 2012).
  • Challenge Protocol: On day 133 post primary vaccination, four cattle in each of vaccination groups 3–6 only were randomly selected, together with two un-vaccinated controls and intradermolingually challenged in two sites with 105 TCID50, 0.1 ml per site, of cattle adapted O1 Lausanne FMDV (Fowler et al., 2012).
  • Efficacy: Conventionally vaccinated cattle were fully protected from experimental challenge, as determined by absence of generalised disease (Table 2). In addition 6 of the 8 DNA vaccinated cattle in groups 4 and 5 (DNA + GMCSF + elec and DNA + elec) were fully protected despite these DNA vaccinated animals being challenged 91 days after the protein boost (Fowler et al., 2012).
2. Foot and mouth disease DNA vaccine pWRMHX encoding a minimal self-cleaving ribozyme
a. Vaccine Ontology ID:
VO_0004320
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse, pig
e. Gene Engineering of FMDV A12 genome
  • Type: DNA vaccine construction
  • Description: This vaccine encoded the genome of FMDV strain A12 and a minimal self-cleaving ribozyme from hepatitis delta virus (Ward et al., 1997),
  • Detailed Gene Information: Click here.
f. Vector:
pWRM (Ward et al., 1997)
g. Immunization Route
Intramuscular injection (i.m.)
h. Pig Response
  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: Swine inoculated with pWRMHX did not show any signs of disease and produced neutralizing antibodies to FMDV, and 20% of the vaccinated animals were protected from FMDV challenge (Ward et al., 1997).
3. Foot-and-Mouth Disease Virus Cationic Microparticle-coated DNA Vaccine
a. Vaccine Ontology ID:
VO_0004552
b. Type:
DNA vaccine
c. Status:
Research
d. Gene Engineering of 1D
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
e. Vector:
pVAC-1D (Reddy et al., 2012)
f. Immunization Route
Intramuscular injection (i.m.)
g. Guinea pig Response
  • Vaccination Protocol: Sixty male guinea pigs of 10 weeks of age were divided in to three groups each containing 20 animals. Group I was inoculated with a 10 µg of pVAC- 1D (naked DNA vaccinated group), group II was inoculated with pVAC-1D coated on PLG microparticles (pVAC-1D + PLG group) and group III received PBS. All the animals were inoculated intramuscularly in the gluteal region of the right leg (Reddy et al., 2012).
  • Vaccine Immune Response Type: VO_0003057
  • Immune Response: Immunization with pVAC 1D-PLG elicited significantly enhanced neutralization antibody responses compared to naked DNA vaccine (Reddy et al., 2012).
  • Efficacy: Microparticles coated plasmid DNA-injected guinea pigs were protected when challenged with FMD virus (Reddy et al., 2012).
4. Foot-and-Mouth Disease Virus DNA Vaccine pSinCMV-Vac-MEG990
a. Vaccine Ontology ID:
VO_0004551
b. Type:
DNA vaccine
c. Status:
Research
d. Antigen
the FMDV-specific MEG990 gene - 3 VP1 genes (Dar et al., 2012)
e. Gene Engineering of 1D
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
f. Gene Engineering of 1D
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
g. Gene Engineering of 1D
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
h. Immunization Route
Intramuscular injection (i.m.)
i. Guinea pig Response
  • Vaccination Protocol: Immunization of guinea pigs with the construct at dose rate of 10, 5, 2 and 1 μg per animal was through an intramuscular route (Dar et al., 2012).
  • Vaccine Immune Response Type: VO_0003057
  • Immune Response: Immune responses included significant neutralizing antibody induction at all doses against all serotypes tested as compared to non-immunized controls, and delayed appearance and reduced severity of FMD lesions (Dar et al., 2012).
  • Challenge Protocol: Viral challenge of guinea pigs was 4 week post-immunization with 1000 GPID(50) of FMDV serotype A (Dar et al., 2012).
  • Efficacy: Two of six and one of six guinea pigs were completely protected in 10 and 5 μg immunized groups (Dar et al., 2012).
V. References
1. Joyappa et al., 2009: Joyappa DH, Kumar CA, Banumathi N, Reddy GR, Suryanarayana VV. Calcium phosphate nanoparticle prepared with foot and mouth disease virus P1-3CD gene construct protects mice and guinea pigs against the challenge virus. Veterinary microbiology. 2009; 139(1-2); 58-66. [PubMed: 19505774].
2. Shao et al., 2005: Shao HJ, Chen L, Su YB. DNA fragment encoding human IL-1beta 163-171 peptide enhances the immune responses elicited in mice by DNA vaccine against foot-and-mouth disease. Veterinary research communications. 2005; 29(1); 35-46. [PubMed: 15727290].
3. Wang et al., 2006: Wang F, He XW, Jiang L, Ren D, He Y, Li DA, Sun SH. Enhanced immunogenicity of microencapsulated multiepitope DNA vaccine encoding T and B cell epitopes of foot-and-mouth disease virus in mice. Vaccine. 2006; 24(12); 2017-2026. [PubMed: 16414158].
4. Ward et al., 1997: Ward G, Rieder E, Mason PW. Plasmid DNA encoding replicating foot-and-mouth disease virus genomes induces antiviral immune responses in swine. Journal of virology. 1997; 71(10); 7442-7447. [PubMed: 9311823].
5. Wiki: Foot and mouth disease: Wiki: Foot and mouth disease [http://en.wikipedia.org/wiki/Foot-and-mouth_disease]