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Aeromonas hydrophila

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Introduction
  2. Vaccine Related Pathogen Genes
    1. aroA (Virmugen)
  3. Vaccine Related Host Genes
    1. CD8A
    2. IFN1
    3. IgM
    4. IL8
    5. TNF-alpha
  4. Vaccine Information
    1. Aeromonas hydrophila aroA mutant vaccine
    2. Aeromonas hydrophila LSA34 vector vaccine WED/pUTDgap
    3. Recombinant Aeromonas hydrophila vaccine (Aera)
  5. References
I. General Information
1. NCBI Taxonomy ID:
644
2. Introduction
Aeromonas hydrophila, which belongs to the genus Aeromonas, is a rod-shaped, facultative anaerobic bacterium that inhabits aquatic environments. Optimal growth occurs at 28°C. Some strains of A. hydrophila can cause disease in fish, amphibians, and humans (Wiki: Aeromonas Hydrophila).
1. aroA
  • Gene Name : aroA
  • Sequence Strain (Species/Organism) : Aeromonas hydrophila subsp. hydrophila ATCC 7966
  • NCBI Gene ID : 4489425
  • NCBI Protein GI : 117619998
  • Locus Tag : AHA_1979
  • Genbank Accession : CP000462
  • Protein Accession : YP_856510
  • Taxonomy ID : 7966
  • Gene Starting Position : 2172700
  • Gene Ending Position : 2174067
  • Gene Strand (Orientation) : +
  • Protein Name : 3-phosphoshikimate 1-carboxyvinyltransferase
  • Protein pI : 7.25
  • Protein Weight : 46562.74
  • Protein Length : 455
  • Protein Note : catalyzes the formation of 5-O-(1-carboxyvinyl)-3-phosphoshikimate from phosphoenolpyruvate and 3-phosphoshikimate in tryptophan biosynthesis
  • DNA Sequence : Show Sequence
    >gi|117617447:2172700-2174067 Aeromonas hydrophila subsp. hydrophila ATCC 7966, complete genome
    TTTGTGCCACCCGCAGTCTTGCCGAGCAGGGCGCGGGCTGCTAACGTCCGGTCAGGTCATTTTTTTGAGA
    TCGTACAGGAAGTCTATGAATTCGTTGCGTTTGGAACCTATTTCTCGTGTGGCCGGTGAGGTCAATCTGC
    CCGGCTCCAAGAGCGTGTCCAACCGGGCCCTGCTGCTGGCAGCCCTGGCCCGTGGCACCACCCGGCTCAC
    CAACCTGCTCGACAGCGATGACATTCGTCACATGCTGGCGGCGCTGACCCAGCTCGGGGTCAAGTACAAG
    CTCTCCGCCGACAAGACCGAGTGCACCGTGCACGGTCTGGGTCGCAGCTTCGCGGTGAAGGAGCCGGTCA
    ATCTGTTCCTCGGCAACGCAGGCACCGCCATGCGACCGCTGTGCGCCGCCCTGTGTCTGGGCTCCGGCGA
    ATACACCCTGGGCGGGGAACCGCGCATGGAAGAGCGCCCCATCGGCCATCTGGTGGATGCTCTGCGCGAA
    GCGGGCGCCCATATCCAGTACCTGAAGAAAGACGGTTATCCGCCGCTGGTGGTGGATGCCAAGGGGCTAT
    GGGGCGGCGATGTGCACGTCGACGGCTCTGTCTCCAGCCAGTTCCTGACGGCGTTTTTGATGGCGGCGCC
    GATGGCGGCGGGCGATACCCGCATCCACATCAAGGGGGAGCTGGTCTCCAAGCCCTACATCGACATCACC
    CTGCACATCATGAAGCAGTTCGGGGTGGTCATCGAGCACGACAACTACAAGCTGTTCTACATCAAGGGCA
    ACCAGAGCTACGTCAGCCCGGGTGACTTCCTGGTGGAAGGGGATGCCTCCAGCGCCTCCTACTTCCTCGC
    GGCGGGCGCCATCAAGGGCAAGGTGCGGGTTACCGGCATCGGCAAGCACAGCATTCAGGGCGACATCCAC
    TTCGCCGACGTGCTGGAGAAGATGGGCGCGCGCATCACCTGGGGCGATGACTTCATCGAGGCCGAGCAGG
    CGCCGCTGCACGGCATCGACATGGACATGAACCACATCCCCGATGCGGCCATGACCATCGCCGTGGCCGC
    GCTGTTCGCCGAGGGGCCCACCTCGATTCGCAACATCTACAACTGGCGGGTGAAGGAGACCGACCGCCTG
    CACGCCATGGCGACCGAGCTGCGCAAGCTGGGCGTCGAGGTGGAGGAGGGGCGTGACTTCATCACCGTTA
    CCCCGCCAGCGCAGCTCAAGCATGCGGCCATCGATACCTACAACGACCACCGCATCGCCATGTGCTTCTC
    GCTGGTGGCGCTGTCCGATACCGCCGTCACCATCAATGATCCGGGTTGTACCTCCAAGACTTTCCCGGAC
    TACTTCGACAAGCTGGCCAGCATCAGTCAGCGCGCGTG
  • Protein Sequence : Show Sequence
    >gi|117619998|ref|YP_856510.1| 3-phosphoshikimate 1-carboxyvinyltransferase [Aeromonas hydrophila subsp. hydrophila ATCC 7966]
    MCHPQSCRAGRGLLTSGQVIFLRSYRKSMNSLRLEPISRVAGEVNLPGSKSVSNRALLLAALARGTTRLT
    NLLDSDDIRHMLAALTQLGVKYKLSADKTECTVHGLGRSFAVKEPVNLFLGNAGTAMRPLCAALCLGSGE
    YTLGGEPRMEERPIGHLVDALREAGAHIQYLKKDGYPPLVVDAKGLWGGDVHVDGSVSSQFLTAFLMAAP
    MAAGDTRIHIKGELVSKPYIDITLHIMKQFGVVIEHDNYKLFYIKGNQSYVSPGDFLVEGDASSASYFLA
    AGAIKGKVRVTGIGKHSIQGDIHFADVLEKMGARITWGDDFIEAEQAPLHGIDMDMNHIPDAAMTIAVAA
    LFAEGPTSIRNIYNWRVKETDRLHAMATELRKLGVEVEEGRDFITVTPPAQLKHAAIDTYNDHRIAMCFS
    LVALSDTAVTINDPGCTSKTFPDYFDKLASISQRA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An A. hydrophila aroA mutant is highly attenuated in trout, and a single immunization conferred significant protection from challenge with wild type A. hydrophila (Hernanz et al., 1998).
  • Related Vaccine(s): Aeromonas hydrophila aroA mutant vaccine
1. CD8A
  • Gene Name : CD8A
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 925
  • NCBI Protein GI : 225007536
  • Genbank Accession : ABBA01075791
  • Protein Accession : NP_001139345
  • Taxonomy ID : 9606
  • Chromosome No : 2
  • Gene Starting Position : 86784604
  • Gene Ending Position : 86808395
  • Gene Strand (Orientation) : -
  • Protein Name : transcript variant 3
  • Protein pI : 9.86
  • Protein Weight : 24339.21
  • Protein Length : 235
  • Protein Note : Also known as CD8; MAL; p32; Leu2
  • DNA Sequence : Show Sequence
    >gi|568815596:86784604-86808395 Homo sapiens chromosome 2, GRCh38 Primary Assembly
    ATCGGATGCTGTTTACCCATTAAAGAGGATGCTACAAATGTTTATTCATTAAAGCCACTCATAACAGCAT
    AGTACAACCCTATTTAAAAAAGAAAGACATATTTTACATGTATGTGTAGAAAATAATCAGGAAGAATGTA
    CACCATATTAACAGGAGATTATTCCTAAGGTGAAGAATTATGAGTGGTTCTTATTTCCTTATCTACTTAA
    GTTTTTTCTCTCCAATGGGCAGGCATTGCTTGTACCATACCTTAAGCAAGGAAGTGCATGTTTGGGACAG
    CAGTTTGGGCTCTCAGCCTCCTTAAGAGAGTCAGGTCTGCCTCATCCCTGTATCTGCTAGTTGAGCAGAG
    GCCAGGGCTGTGTGAGGGGCTCTCCAACAATTGTCTTTACAAAGTATAAAAAGTCATCAGTGATCCCAGG
    AACATGTTTGTATCTCAAATTCAGAGATTCAAGAGGGCCTTAGTTTAACCTCACTGATGCTCAAATTCTA
    TTTGTAAAGGGGTAGCCTGTCCTCTTTCATGGGCCCCTCTGCAATGCAAGGGCTGGGAGAGCAATTCCGC
    CTCCACATAGGGGTTTCACAGAGATTTTCTTTAGAGATAGAGGGATTCATTTTCCAGGGTTAAGCTCACC
    ACTTCATTTTATTTTAGGTCTATACAATTTTAGGCTTGATTATAAAAAAAAAAAGTCTGATATTGTTTAC
    ATTATAGAACTCTGCCAAAGGCAGTTCTCTTCTTTAATTCATTACCTCCTCGAGGCTCTGGGCACAGTAT
    CCCAGGTATCAAGAAGTACTTGTTCCCTTGCCGTTGGAGACTCAAGCACCTCACCCTGAGACAGGGGCCT
    CGGAAAGAAAGACCTGAATGGTGTGGAGGAAAGAGCCCTGAGCTGGGAGACAAGGTCCCTCCAGCTACTG
    CTCCAACCCTGACTTGCTGTGTGCCTTTGATCAAGCTGTCTCTGGGCTTTAGCCTCCCCCTTTGTAAAAC
    GGGCGGGGAAGAGGTTGAGATGGCATGGGTGCCTCCAGCTCTCTCAGCATGATTCTGAGAACTCTGCGGG
    TAGCTCTGGCCTGCCCCTTTCCACGCCCTACCGCGATGTGCGCACAACAGTATTGTGACCCTTGTGGTGT
    ACTGTAGATTTTACCTAGTTTTGTTTCCCGTCAAACACATAAAGAAAAAGTAATCTTTCCCACCCCGCCC
    CCACTAAAATAATAATCATGAGAATGAATACACAGGGAGGAAGACTGGAAAAAATGAAAGGGAAGGACTT
    GCTCCCTCAAAAGGAAGGATCTCAGTTTGAAGTAATGTAGTGGCTGTTGCACAGGGTTAGACGTATCTCG
    CCGAAAGGCTGGGCTTGTCTCCCGATTTGACCACAGGCCTGAAAGAGAGGAAAGCGACCATCATTGTAGC
    CAGAACCCCGCCAGTGCAGCATCCATCCAGCCACGTCCAGCCTCCCAATCCCCCAGCCTTTGAAAGGTCT
    GAGAACCTGTTCCTGGGGCACCAGCATGGGACTGGGTGAATGACGTCAGCACAGCACCCGTGCTAAATGC
    CTTACTTGCACCATCTCATGTAATCCTCACAGCAGCCTTAAGGGTGGGCACTGAGATTGAGAAGTTAAAC
    TTGCCCAAGGTCACACATCTAGGAGGTGGCTGAGCCAGGATCTAAACCGAGTTAGTCTAGCTTCCTGGAG
    CCCCTGTCAAGGTCTCCACAGAGGTGTGAGAGTGAGACTGATAACCAAAGTCATTAGCTGACCTGATTCT
    CAGCCCCGGAGGATGACAGGGAGAGAGGAGGATGTGAGCAAATCACCACCATCAGCCAAATGATGTTACG
    CTAAAAACGTGTTAATTCAGCACCAGGCTAGCACCTTGTAAACATGCTAATTCATCATGAGCGTCATGTC
    TGCACTCTCAGCCCTCTAAAACCTCTCTGTTGAAGCTGAGCGGCCCACCATCATCACTGAGTATATTAAC
    TCGGGTGTTGGAATTTCCCAGGTGTGTTTAATCTGTATAGCTTTGTATTTTAAGGCAGAACTTTATTCTC
    AGTTATTCATCTCAAAATGAGAGTTTTCAGAAAAGATCGAGAAAAGGGATGGTCCTTCTGCAAAAGTTCC
    AAAGTGCACCCTGAATAATAAAAATGCTAAAAACTGGCCGGGTGCGGTAGCTCACGCCTATAATCCCAGC
    ACTTTGGGAGGCCAAGGCGGGCGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAA
    CCCCGTCTCTACTAAAAATACAAAAAATTAGCCGGGTGTTGTGGCGGGTGCCTGTAGTCCCAGCTACTTG
    GGAGGTTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATCGCACCACTG
    CACTCAAGCCTGGGCGACATAGCGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAGAAAAGAAAAGAA
    AAGAAAAGCTAAAAACTTCCCCCCCCACTTTTTTTTTTTTTTTTTGTGAGATGGAATCTTGCTCTGTTAC
    CCAGGCTGGAGTGCAGTGGTACGATCTCAGCTCACTGCAACCTCCGCCTCCCACGTTCAAGCGATTCTCC
    TGCCTCAGCCTCCTGAGTAGCCCGGATTATAGGCATGCACCACAATACCCGGCTCATTTTCAAAGTCTTT
    ATCCCCTTGGTATATCACCTGTAACATATGCATTGGAAATCCATTGCATTTCCATTGCTAAAAACAACAA
    TGTGTAAAGTCCTGAACTTGGATTCCCTCAGAAAAATGTAAAGGAGTATAAAATGCAAACTCCAGGTATA
    AAATGTAAAATATTTCATCTACTGAAAAGAACTGGTGTACACAGTCGGTTATCCTTAACACTTCAGCCTG
    CAGCCATGGGGAAGGTGTCTGAGCTTTGGATGGAAGTTGCAGCTCCCGGGCAGTATGACCTTGGACAAAT
    CACTAAATGTTCTAAGCCTCAGTTTCCTTATCTGTAAAATGCAGGTAATCATGACTACTTTAAAAGTTGT
    CATAAAACTTAAAATTAAAAAAAATCTATAAAGCAAGTAACGTGCGTTGTAGGCTTTCATCAAATGGTTA
    TTACTATTGATAATCTGAGTTTAATGTGCCTTTGCCACTTTACTAATCCCTGAGCCATGAGATGGCACAA
    AGTTGCTGTGAGGATTAAGAGAGATGGGGAGTATTACCCCTATCACAGTGCCTGACTAGAGCAGGCACTT
    GTTAGATGCTACCACAGGGATGCCTCAGCCAACGGGTGGAGAGAGGTTTTTAACAGAGAGGGAGAGAGAG
    AGAGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATCATGGGCTGAAACTGGATGAGGCAGTCACTTGAG
    CTGCCAGATAGCCAATTGTCCCTGCATCTCGTTCCCCTTTTTTCTACTCCAGTCCTTGGCAGCAAACCAA
    GGTCCTACATGACACAGAGAAGTTAGCAACCAAAGCCATTATTATTTTGGTTCAGCTGATAAAAGATTGG
    CTCTCCCTCAAAAAACTAGACCAGCCAAGTAGGAAAAAAAATTGTTTTCTTAAGAAAACAACAAAACCCA
    GATGGAATAATCAGAATAAAGAGGAGGTCAGGTCCTACGAGGAGCTAGGATAAATTGGGGGAATCCCTCA
    GGTTTTTTTTTTTTTTTTTTGGAAGTGCCAGGTCAAATAAGATGGAGATTTACTGGAGTCACACTGCTGT
    TGGCACCCGCCTAACCCGGGCCTCCTTTTTCTCAAAGACACAGGCCAGATGCCAGCCTTGGCCTCTCTTT
    GCTCAGGAACTGGCTGACTCCCTGCCCCAGGATTTCACGGCCATGACCTCTCTCTGGGAAAAGTTCCCAC
    TATTTGCTGCTGGGGAAACCAGGACCCCACCCCAGGGCTGGCCAAGGTGAGCAGGCTGAGTTCAAAAGAG
    ACTCACCGGGGACATTTGCAAACACGTCTTCGGTTCCCTGGATAAGGAAAAAGAAGGGAAAAAGTGAGTG
    CCCCTATCCATCAATAGTCCCCAAAGACAGTGTATTTTTTGTTGTTGCTGTTGTTGTTGCTGCTGTTTTT
    TGGTTTTACAAAAAATCATTTCAGAACGATCATGGGCTGAAATTGGATGAGGCAAATAGGGAATTGCCTC
    TACGAATTCTCCTTCCTTTCCCCTATTTCTTTGAGGATTATAACTCTTGAGAGCCGGGTAAGGGCTTCTT
    ATCCATCCTTCGTCTCAAGCATCAATTAAAAAAAATTGCAACTACAGACGCGGGCGAAAGCGGGGAGCCT
    GCAGACCTGATCCGGCACCTCTCTTGCATGGGACCCAAAGCAGGGTGGGCGGTTCGCCGAAGAGGGCCAA
    GGACGAACCTCAAACTGATGGTGGTTTTGACTAACCCTCCTGGGCACTTGACTCGGCCACAGCGGCCCCC
    TCCCGGCGGGCGTTTGTCATGTGTCCAGACATGTGCGCGCGCGCTGCGGGCTTCGTCGACGGAACACACC
    CGCTGCAGCGGCTCTGGGAACGCGGCTTTGTCTCCCAAAACCTCTCCGAGAGCGCAAGGAGCGGGAAGGG
    CTTTCTCGCATTCTATCTCCCAGAAAGAAGTGCCTGTACCTGCGGGGCAGCTCGGGAGTCCCAGAAAACT
    CAACCCCAAGCTCCCCCCTCGCAAGGTCCGCCTGGAGCTAGCAGAGCCAAGGGCAGGAGCGGGGCCGACT
    CCTTCCCGCCGCGATTCCTCGGGACTTACTGTGGTTGCAGTAAAGGGTGATAACCAGTGACAGGAGAAGG
    ACCCCACAAGTCCCGGCCAAGGGCGCCCAGATGTAGATATCACAGGCGAAGTCCAGCCCCCTCGTGTGCA
    CTGACGACACCAAAGACGCCGACATTTAGGAGAGGGCCCGGGACCTCCCAACCGCCCCACCGTCCCGGGA
    ACGTCTCTCCGCCTCAGATCTCGGTTTCCCACCACTTGGACAGCCCTTGACTCTACCTACAGTATCAGGG
    CTGTCCCTGGCATGGGCTCTCCCCGCGGTGCGTGCCGCCCCCGCCCCGGGCCCCCGCACGCCTCACCTGC
    GCCCCCCGCCGCTGGCCGGCACGCCTCTGGGCGCAGGGACAGGGGCTGCGACGCGATGGTGGGCGCCGGT
    GTTGGTGGTCGCGGCGCTGGCGTCGTGGTGGGCTTCGCTGCAAGAGCAACAGAGCGTGGTTGGGGGCCAG
    GCTGGGGTTATGGAGGCGCCCCAGCCCCGGCCTCGCGCACCTTTCCCCACGGGGACGCCTCCCCCCGGTT
    TTCCTGGGAGAAGGGATAGCAGAGGAGACAGGATGGGGACCCCGGGATGCGCGCGGACCCCTGTGCTCGG
    GCCTCGGCTCAGCCCAGCGCACCCGGCGCCCAGAGCCGGAGCGCAGGAGCCAGCTCCCCTGCACCTGGCC
    TCTCTCCCGGGCTGAACCAGCAACCCTGGAGCGCGGGTTGATTGCTGTCCGCATTTTACCCACGAGCAAA
    CGGAGGCGCAGATAATTTAAGTCATTTGCCCAGAATCACACAAGAATCCGAACTCGAGTCCAGATCGGCC
    GGACGTAAAAAGCCATCCCCAGTCCCTACTCAGCCTCGCGTTAGCCTCAGTTTGCTGTGTCTGTGAAATG
    GGAACAGTATCTAAGTCGCTTCCAGGTGCGCTAAGAGGCTTGAAAGCAGGGCCCAGGTGTGGAAAACAGG
    TTGAGGTGAACCCCAAGCCCCACGCGGAGAGGTGCCGCAACCCGGCGCGCGGACCTGGCAGGAAGACCGG
    CACGAAGTGGCTGAAGTACATGATGGAGTTGCTCAGGGCCGAGCAGAAATAGTAGCCCTCGTTCTCTCGG
    CGGAAGTCGCTCAGGGTGAGGACGAAGGTGTCCCCCAACCTCTTGCCCGAGAACCGCTGGGTGTCCAGCC
    CCTCGGCCGCCTTGGGCTTGTTTTGGGAGAGGTATAGGAGGAAGGTGGGACTGGCGGCGGCGCCGCGCGG
    CTGGAAGAGCCACGAGCAGCCCGACGTCGGGTTGGACAGCAGCACCTGGCACTTCAGCTCCACTGTCTCG
    CCCAGGTTCCAGGTCCGATCCAGCGGCGACACCCGGAACTGGCTCGGCCTGGCGGCGTCTGCAGGCGGCA
    AGCAGCGAGGCTGAGCCCGCAGTCCCGCGCCCCCCGCCCCCCGCCCGCCCCATCCCCTGCCTTCCCGGGC
    GTCTCAAACTCACGGAGCAGCAAGGCCAGCGGCAGGAGCAAGGCGGTCACTGGTAAGGCCATGACGCGCT
    CCCCAGGACGCTGCTTGGCTCGAAGCTCGGGCGCGAGGGGAGGCGCGCGGGAGCCGGTGGGGCGCCGAGG
    GGGGAAAGTTGCGCCCTTCGGCCGGCCCGGAGCCTGATTTCGCATTTGGAGGATGTGATGTCACCCGAAG
    CCCCCGCCGAGGAGAGTCACCCTCCTTTTCGCGGTTGTCGCCTTCCAGCCCGGCGAGGAGGCTGGGGCCC
    GTGAATAGGGCCGTCGAGGCAGCCTGGCCAGGCAACTGGGGGCAGCTGAAAACTGCGGGTTTGGGGATGA
    GGAAAAGGGCTTGGAAATAGTCCTTGGAAATGGTTGTCTTGTGAGAGTGACAGAGTGGGTGAAGGGAGAC
    CAAAGATTTCAAGAAGTGAGGGCGAGAGTAGGCAGCAAAGGAGGGGAGTGTCCCTTCCTTTGCCTTCACT
    AAAGGCGTCTCTTGTACTGTCACCTTGGGACTTTTTATTGGCAAAATGGGCACTGAGGGCTGAAAAGGAA
    GAGGAACTAGCGACCTGCCCGCTTCTGAGGAACTCGCTAGAGCAGCCCCAGTTTTCACCGAGGAAGGACC
    CTCTCCCTTCCCCCAGGAGATTTCCATGAGAGCGGCAGCAGCCGAAGCTTTGGGTGTCGGTGTCAGTGCG
    CTGCTGACCTCATTCTTCCGGCCTTTCATCCAGCGGCTAAACTCACCACACAGCCTCATCTCTTCTTGGA
    GCCATTGCAACAGCCTTCAATTCACACCAGTCTCTCTCCTGATCAGTCCTCCAGCCACGTTGTTATAAAA
    ATTATTATTCTCACAAAGGGGATCTGACAGCGTCACTGCTGCAGCCTCCCATGGCCTGCATTGCTGGATG
    GAAATTCAACCCCCAGCTTGGTTGACCAGCCCTTGGGTGCTGGCTCCTCCCAGCCTCCGCACAGCCCCCT
    GCCCTGCCTTTCCCATACACCTGCAGCTGCAGCTTCACAGGCTTGAAGTCATTCAACCCCTGCGCTCTGT
    GCTTTCACGCCCTTGCACTTGCGGTCCCTCGGCCTGGAATGCTGGTCTGTTTAAGCCCTGGAGGCAGCTC
    CTGTGCTGTCTCATTTGGACCTCATTTCTTTCACCCCCAGCTCTGGGTGCTCTGCAGGGAGGTGTGGGTG
    ATGTGGGTGAATGCTTGGGTAGAGGCGGTTTTCTCTTTTCTGTCCCTAACACATGTGTGTCCCCTTCCTT
    TTTGTTTCTTTAAAAAATAAAATAAAATAAAAAAGACTGGAGTCTCGCTGTTTTGCTCAGGCTGGCCTTG
    GGACTCCTGAGCTCCAGTGATCCTCCCACCCCAGCCCCCTGAGTAGCTGGGACTACAGGCTCATCTTTCC
    TTTTTGACTGTTGCTGTCCTGAAACCTTCCAGCTCCAGTGGCATGCCCTCTTCCCATTTCTTCAGGGTTT
    CTGAGTCTTTCCACTTTGAATCCCATAGTGGCTGCACCTCACATTGCTACTGCTTGATCCTAGAGACAAG
    ACACCTGGCATTCTGTGTTCCATGTGTCTGCCCCACCCATTAGTTCTGCTGGGCGAGGACCATGCCGAGA
    CTTCATGATGTGTCCTCCATCCCCAGAAACACAGCATGGGCTGAGCTGTGCTTGTTGAACTGCATTCTCC
    TTGTCCTTGTGAAGTACAAACTTAAGTGTTGGATGGGTTCTTTGTCGTTGTTGCTATTTTTTTTTTTGAG
    ATGGGGTCTGGCTGTGTTGCCCAGGCTGGAGTGCAGTGTGGCACCACCTCGGCTCACTGCAGCCTCTACC
    TCCCAGGCTCAAGTGATCCTTCCACCTCAGCCTCCCAAGTAGCTGGGACCACAGGAGCACACCACCATGC
    CCAGCTAATTTTTTGTATTTTTGGTAGAGATGGGGTTTCACCATGTTGCCCAGGCTGGTCTCCAATGCCC
    GAGGTCAGGCGCTTTGCCCCGCCTTGGCCTCCAAAGTGTTGAGATTACAGGTGTGAGCCACCATATCAGG
    GCCTGGATGGGCTTTTTTTTTTTTTTTTAATTATTTTGATTTCAGTACCTCCTTCCAGCTCCCTCAAAGC
    ATCCAAGATGCTTATTCTAGCATGGAACATGTAACATTTTATATTCAGAAATTTTTAAAAATGTCTTTTA
    AGTGACAACAGTGCAATATATTCTTGTACGAAAACAAACACAAAGTTCCCTGTCTCAACTCTCCTCACTG
    TCACCTGCTCCCCACAGAAAACCACTGTTACAGTTGAATGTGTCCCTTAGATATTATCTGTACATAAACA
    GATGTTTATACATCATCTTAGACACATGGGATTGTATGAGATAAATATTTTTTTGCAACCTATCTTGTTT
    CACAGTAGAAGGAGAGCGTTCAGCAGATGAAGATCCACCCCATTCTTATAAATGGGTGCATTATATATGG
    GTGTTCCATAATTTAATTCTGCTGTTAGTGGACATTTAGGCGATTTCTCATTATTGCAACACTAGCAAGG
    CTGTGGTAGATATAATGTATCTGTATCACTGAGGGCTTGCACCAGTACCTGCAGCTTTTTCAAATGGGAG
    ATGAGGGAGTTGGGGAAGAAAACACCTTTACAATGATGGTTATAACTGGCCTATCGATGTATGCATGTGT
    GTGTGTGTGTGCATGGTATGAGGGTCCCTCGCTGTGTAGACACAGTGCTGTCCCTCAACTCACATGAATG
    AGGGAGACAAGGAAGCAATCAAGTCCAGACAGGCCACCAACACATCAATTCTTGCTGTAGATGGTAGTTT
    GCCATTTTGCTCAGCACCACCAGCACTGTATCAGGAGTGTCAAATGGCCCTGCTGGCCAAAGGCTACAAT
    CAACAGGGTGGTAAGGAAGATGGCTAGACACGAAACTGCACTTGCCCAGTGGTGGAAGCACTGAGGGGGC
    CAGAGTCAACCTCCACCTATTTGGATCTTGAGAATCAGGCCCCTGAGTGTGATGATGGCCAGATTTGGGG
    GTGTACTGCTTTAAAATAGACTAGTTCCATCTTAGTCTCACGGAAATCAGCTTGGGGGCCTTCTAGCCCT
    GCAGCTCAGAAAAGTGTCAGCCAGTGGGGTGGCAGCCCCTTTGTACAGAGCACCATGGGGGTTGGGGTGG
    GGAGGATAAGGCAACATGTCAAACCCATCAAGGAGGCTTTGTGACCCCAGTGATATTTTTTGCAGAACGT
    GGTGGATTTTCGATGTGACCACAATGACATCCGCCTTGCAGTGGCAGAACAGATGCAATTGCACAAGTTC
    TGGAGAAACTTTCTGTAGACCAGGTGTGCAGAGGGCTGGGTTGGGCCTGTCCATCACTGCCAGGTAAGTT
    CCCAAACCCCACACTGTAGCACTGACTTGGGATGGCATGAAGAAATGTGAATGACTGCACTCCTTTAAGC
    AAGCCCCTTATTTAAAAAAATATCCAGAAGTAGATAGAGTAAGATAGTAACCAGTTTCTATGCTGATTTA
    AACTGGTCCATGGAAATGGAAAACTTTAATGTAGATTAAAGACATATTCAAAATGCAAAACCGGGCAGAG
    CAGTGGTAGGAAATCCATCAGGGTTCTGTGCCACTGCTCCTAAGACATGAACTGCAGGGACAACTTGGAA
    TCATGGCTGGGAAATTGCCTGCTGTGTCCCCATCGCCAATTCAGGTCTATGGAGATGTTACTGTTCTTGG
    AAGGAGTTAGCTTGTCCAGGGCTAGAAACTCTAGAGTTATCAGGGCTTGTTTTATCTTAATTGGAAATAC
    CAATCAATTTGGCCTCACTTCTGTCACCTTTCTTTTCCTCCACCCCGAGTTCCCCAAGTTCATCTAGCTG
    CTGTCCTTCTTTCCTGGACTTTTGCAATGGCCTCTTAACTGGCCAACCCCATTTCTGCTCTGGTCCCTTC
    TAGAGAAAATCTCATCATGCCAACCCCCTGCTTCAACCTGAAAGGACTTCCTGGTGTCCCTAGTAGAATG
    TCGGGGATCCCTAATGTTGCCTGTGAGGCCCTGCACACTTGGTTTCATAGTCTCTGTCCTCTTGTCTGTC
    ACCTTCTTCACTCCTCCTAGGTTCCGCATCTCTCATTTCTCAAGCGGACCCTAGTCCTTTCCACTTCAGA
    GCCATTAAACATGCTATTCCCTCAGCCAAGAATGTTCTCACCTCTGCTCTCTGCTTACCAAACTCCTCTC
    CCTTAAGACCCAGCTCAAGCATCTCCTTCCTGTGGGGTTTAGCTCCTTCCCCCTCCCACTCCCAGACAGT
    ACAGACCACATCCTTCTCTTCTCTGTGTCACCCGGACCTTGGGTATCTGCAGACTGGAGCACAATGGTTT
    GTTTGCCTGTCCAGCTTGACAACCAAACCCTTGGTCCCTTGAGGAAGGAGCCTTGTCCCTCTCATGTTTA
    CACCAGGGTGCCAAACAGAGGGCTTGGCACACACTTGAGTGGCACACACTCAAGAAATTCTTGCTCAGTA
    AAAGCTAAAGGAAGGTAGAAGTAGGGTACAGCAGACCAGAGGAGGCTTAGATAGTTCATTCCAAGTAAGA
    GTGGTCAGGAAAGGCTTCACATATAGTTGGGTTTGAAGATAAAGGAAGAATTAGGAGAGAGGTTGTTTAG
    AATGAGGCATTTGATATGTCTGATCAAGATCAGCTGATACCATGCTTTAACATATAGCAATGGTATAGTT
    AAAAACAAAAGAAGAGGAGGAAGGGGAGAAGGCAGATGTACTCAAAAGCAAGATAAACGTCTTCAAGGAT
    GTAGTTGCAGGACAATGGCTGGAATCAGGGGCTGGCTTGCTGGGATCTGTTCTTAGCAAACCATTCTTGG
    GCCTGGGTCTTGAGGGTCTGTTCTTAGTGTAGAGGCACCAAGAGTTTGACTCCTTCAGCTAACAGAGTTT
    CACAGTGCTTTGCCTATAGAGGAAGGCTGGAGCCAGGCTCTCTGCTTGAAACAGGGATTTGGATCTGATT
    TTCTCTTGATTGAAAGAGAGCCCACAGCCACCCATGTAAGGCCTAGTTCTGACTGTGGGCCCTGGAAAGT
    CAGAGGCTACTTTAAAAAACTCAGGAAAAGAGAGAGTAAAGAGAGGAAGAGATTTTCAAAAATTTCTATT
    CAAGATAACCCTGCACACACACTCGAAATTCAAAGTATACATAAAAAAGACTCAACAAGTGGAATGTGAA
    TTCACTTCCAAAAAGAGGCAAATTATTGAACAATTTGAAAAATGTTTAAAACAAAGAGAACAACAAATGT
    AGAAAATGCAGAAATTAAAGAAGAACAGTTGAAGATGATAAATACTGAGCATACTGGAAATGAACACTCT
    ACATATTAAACAAGGATATACTCTAGGTTGGACATAGTTGAAGGGATAATTAATGAATTGGAAGCTAGCA
    TTGAGAAATTCATAGAGAATGCAACACAGGGAGATGAAGACAGAAGCAGTTTGAAAAAGCTGTTGAGATG
    TATATTAGCATGGTGAAGTGTTTAATAGGATTTCAGAAAGGGGAGAACAAAGGGAATGTGGAGAAGAAAT
    ATGGGAAGAAAGCATGGCTGAAAATTTTTCACAATTAAAGACAAACATTCTCAGATCAAAAATACTCTTA
    GTGCCAAGTATAAAAACATATACATCTTTGCACATATCAAGGTGAATTATAAACATCAAGAGTAAAGAGA
    AGAGGGCATCCCAGGAAGCTAGGATGTTTGAAATATAATTGGGAGAATCCTTGTTGCAGTTTGGATTGGG
    CAGAGAGGGTAAGAAGCTGAACTGTAATGGGGAAAAATATACACATTTTGGTTACACCTTGCATTGACTC
    TCTGTCATCCCCTCCCTTGGTGCTGACACATGCTTACTGTAAAACTGATCCAATGATCCCATAGAGTTGA
    TGTTTGTGGTTTCTTTGAATAAACATATAAATTGATCCTTGCCTTCTTAAAACCTGAGAAAGTTACATTT
    GTCTTATCTGAGTTCCTTTCTAGGGAAACCAACTATCAGGCCTCCCAGATGGTAGCAATGAGCTGAAACT
    CACCAGATCACTTATTATGAACAATAAGACGTCAGACTCTTCACCTGGTATGATGGCCTAACTAACCTCC
    TGTTTCCTGTTGTCCAACTCCTTTTCCTTACTCCTCCCAACTTCCTGTTTTCCCACACATGATTACATGT
    CTTCCCTGCTATATAAACCCCTTAATTTTAGTCAGGTCAGAGGGATGGATTTGAGACTGATCTCCTATCT
    CCTTGGCTGCAGCACCTGACTAAATTCTTCTTCCTTGGCAATACTTGTTGTCTCAGTGATTGGCTTTCTG
    TGAGGTGAGCAGAAGGACCTAGATGGATCCCCCGGTGTTTCGGTAACAAAATTGTTTTGGATCTTTAATC
    TCAGTAACACTGAAACAGAAGCGTTCTGAATGCAACTGGAGAGCCAGGATTTGAGTTCAGAAGAGGGGTC
    AGGGCTGGGGATGTAGCCTTGGGAAGGAGGTGATAAGTTGTGAGAGTGGGTGTCATTGCAAAGGGAGGTG
    GTGTGGAAAGAACATACAGAAGAGTATATGGAACCTTCAGGGAAGGCTCGTATTGAAGAGCAGGAAATAA
    AACATTGGCAAGGAATCCAGGTAGTGCAGGAAATTCAGAATGGGGCTTGAGAAGGTGGAAGAACTTCACA
    GGGGAGGTTTGGGCTATAAGAGAAGCACAGAGAGGACATCAAAGCCTGTGGCAGGTAGAGATTGTAAGCT
    CATGTCTGCCCCTGACCCACATGAGGATGTCCCACATGTTTGCCATTGGTGGATGATAAGATGAGGAGGA
    CTCACAGGAGAAAGGCCAACAAGGGAGGGTAGGGGCAGCATGGGGACTTGTGGAGGGCAATGTCAGATGA
    AGTGATGTTGACTTCTGAACTTCATTCATTATTCACTCATTTAAATATTTCTGCTATTCACTTTTTTAAA
    AAAAAGTTTTGAAAACATTTTAACATAGAACAATATGTCAAATAATATAAAATGCCCACTCTTTATAAGT
    GTTAGCATTTTGTCGCATTTCCTTCACATGAACATTACAGATAAAACTGTACCCTCCCAAGTACCACCCC
    ACCCGCCTTCTCATGAAAAGCTAAATTTGGAGTTTTGTTCATTAATTATGACAGAAAGATGGTAAGTTTT
    TTAATTTTTAATCTGTTAACCTTTCCTATGGAGCTGAGAGAGAAGGATGCCGAATGTCCAATGAGTGTGG
    ATTTGTCTATTTCTCCTTTTAATTCAGTTCATATTTTGTCTCCTATATTTTGAAGCTTTGTTATTAGGTG
    CATACACATTTAGAATTGTGCCTTCTGGATGAATTCACTCTTTTATCATTATGAAATGTTCGTCTTTACC
    TCTGCCTCTGGTAATACTTTGTTGTGAAGTCTACCTTTTATTCATAAGGAACAGAGCTTTCTTTCTTTTT
    TTTTTTTTTGAGATGGAGTTTCCCTCTTGTTGCTCAGGTTGGAGTGCAATGGCACGATGTTGGCTCACCG
    CAACCTCCACCTCCCAGGTTCAAGCTATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATG
    CACCACCACGTCCGGCTAATTTTGTATTTTTAGTAGAGACAGGGTTTCTCCATGTTGGTCAGGCTGGTCT
    CGAACTCCCAACCTCAGGTGATCCACCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCAC
    TGTGCCCGGCCAGAAACACAACTTTCTTATGCTTCCCGTTTGCATAATGTATCTTTTCCTTTTTCTTTTT
    TTTTTTTGAGGCAGAGTCTAGCTCTGTTGCCCAGGCTGCAGTGCAGTGGTGCGATCTCATCTCACTGCAA
    CCTTTGCCTCCAGGGTTCAAGCAATTCTCCTGTCTCAGCCTCCTGAGTAGCTAGGACTACAGGTGCACGG
    CACCATGCCCAGCTAATTTTTATATTTTTAGTAGAGATGGGGTTTCACTATGTTGGCCAGGCTGGTTTTG
    AACTCCTGACCTCAGGCAATCTGCCCACCTGGCCTTCCAAAGTGCTGGGATTACAGGCATGAACCACTGT
    GCCCAGCCTGCATAATGTATCTTTTTCTATCCATTTACTTTCAACTTCCCCTTCTCTGTTTTGAAATGTT
    TACTAGATATGTATACATGTATATGTGTATATATATCTATAAATGCTATATAATTTTATTTTCTGAGAGT
    TTCAAAATACAGAAATGTCATCATATTGTGTGTCTTTGAGCTATGTTGATACATATAGATTTATTTTAAA
    TGCTGTGTGGTGTATTATCCTGTGAATGTGCCACAGTTTCATGTTCAGTGGTTCTTGAACCAGAGTGTTC
    ATCTCTCTGGAAGTATATGGAGGCTGTCCTTGGGGATACACATAATTTTAAGGAAATTGTTTTCCAGATT
    CTCAACTCCCATTGGTTCTCTTCTCATAAAATGGACCTGCCCAAGAACAAGTCCCTGTGTTTAGGCTTTG
    TGCTCGTTCTCCTTTCTGGCCTATTCTTTCATGATTATTTTATTTCCCACTTTAAAACTGAAAGGCAAAC
    TTCCCGCCCTCCTGTTCCTTACTATGCTCCAGAGGTGGGCACGTAATCCTCTGGGCCACCAATAAAAAGA
    CAAATCCAAACATTTTTCATGTAATTTCCATCCGTTAGAAATTGCCAAAGAATGCATTTCTCCTGAGCAG
    AGTCCCCAGTATCCAGGTAGAGTCATCTGGGGGCCGGGCACGGTGGCTCACGCTTGTAATCCCAGCACTT
    TGGAAGGCCGAGGTGGGCGGATCACGAAGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCC
    ATCTCTACCAAAAATACAAAAAATTAGCCGGGCACGGTGGCGGGCGCCTGTAGTCCCAGCGACTCGGGAG
    GCTGCGGCAGGAGAATGGCTTGAACCCGGGAAGTGGAGCTTCCAGTGAGCCAAGATCACACCACTGCACT
    CCAGCCTGGGCGACAGAGCGAGACTCTGTCTCAAAAAAAAAAGAAAGAAAAGAGTCATCTGGGAGGGATG
    CAGGAGGCAGAGTGCCTCACAGCAGGCTTTGGGGCCTCATCCCTCTACTCAGCTCTCTATCATCGAGCTA
    GAATTCAGAGGTGAGACAGTCATTATGAGGATGCATATTAACGTGTTCATTATGATTACAAAAGGAATCA
    GAATTCTTTTTTTTTTTTTTAACAGAAATTAAGTCTGAGTTGTTAATTGGTTTGACATTGAAGACCAGCT
    TTGCCAATGAGGTTAATGGCAGTATTTTAACTTTCCTTGAATCTAGTGAGCTAAATCTTTAGCTCCAAGG
    TTTTGATGAGAATCAAGTTTAGCGCTAAAGAAACAGCTTAGAAAAAGAAAGAAAAGAAAACACTTTTAAC
    AAGTTTAAAATCTATGATAAGATAAAAGCATTTTGGCCAGGTGCGGTGGCTCATGCCTGTAATCCCAGCA
    CTCTGGGAGGCCGCAGTGGGTGGATCACAAGGTCAGGAGTTTGAGACTAGCCTGGCCAACATGGTGAAAC
    CCCGTCTCTACTAAAAATACAAAGAAAATTTAGTCGGGCATGGTGGCAAGCGCCTGTAATCCCAGCTACT
    CAGGAGGCTGAGGCAGGAGAATTGCTTGAACCCAGGAGGTGGAGGTAGCAGTGAGCCGAGATCACACCAC
    TGCACTCCAGCCTGGGCGACAGAAAAAAAAAAAAAGAAAGAAAAAAAAAGATAAAAACATTATATCACAA
    TATTGTTTGTATTGGAATTTGTCTTGAAATTAAATTAAAAATATTTCTAATACCCCAACACTTTCCAAGT
    TAATCAATTTCAGCAATCGTTTTTAAGTGAAAGAAGAATGATGTCATTGATGGCTACTTGGTAGGCTTCG
    GTAAAACCTAGTTTAGGGCTTCCAAAACCCAAGAAAGTGAATAAATCTAAAAATGGTAACAAACCCATGT
    GCAGGTCAGTGGTTCCCAAGTGTTTGCTTTCAACAAAATTGGAAGAAGGCCTTCTAGATTTGGCAACTAT
    CACAGGTTGAATTGTGTCCCCCCAAGTATATTTGTTGAAGTCTTATACTCTAGCATCTCAGAATGTGACC
    TTATTTGGAGACAAGGTCCTTACAGAGGTAATCAAGTTCAAATGAGGCCAATAGGATGGGTCCTAATCCA
    ATATAACTGGTGTCCTTGTGGAAAGGGGCAATCAGGACACACACACAGAGAACATCATCTGAAAACAAGG
    CAGAGATGGGGTGATGTTTCTACAAGTCAAGGAATGCCAAAGATTGCCAGCAACTGACTAGAAGCTAGGT
    GAGAAGCATGGAACAAATTCCTTCTGTAGCCCTCAGAAGGAACACACCCAGCTGACACCTTGATCCTGAA
    CTTCTAGCTTCTAGAACTGTGAGGCAATAAATTTCTTTTGTTGAAGCCACCTAGTTTGTGGTACTTTGTT
    AAAACAGCCCTGGCAAACTAATACAGCAACTAATAGATCATTAAAATTAATTTTTGATGATAGATAACAT
    TGTCTCCCTCCCTCCCTGCCTCCCTCCCTTCCTTCCTTCCTTCCCTCCTTCCCGCTCTCTTTCTCTCTCT
    CTTTTTCCCTTTCTCCCTTTCTCTCTCTCTCTCTCTTTCTCCCTTGGAGAAAGGGTCTTGCACTTTCGCC
    CAGGCTGGAGTGCAGTGGAGTCACTGCAGCCTCAACCTCCTGGGCTCAAACAGTCCTCCCACCTCAGCCT
    CCCAAGTAGCTGGGACTATGGGTGCACACCATCATACCTGGCTAAATTTTTGTAGAGATGGGGGTTCACC
    ATGTTGCCCAGGCTGGTCTTGAACTCCTGGACTCAAGGAATCCATCCATCCACCTTGGCCTCCAAAATGC
    TGGGATTACAGACGTGAGCCACTGCACCTGGTTCATGCTTTTGTCTTTTTGAAAAAAAAAGGAAAAAGTT
    AAAAGATTTAGTGATTCTGCTGTAACAAAGCCAACTATTTATTTATGGAGACAAAATTTCTCAGTACTTA
    CATTGTATGACAAACAAAAGCAAAACAAAATTAATACTGAACTCTGTCTCATTCTAGCAATCAATAAATA
    AAATAAATCCTGTTATTTTATGGGTTCATTAATTAATTGGACCACAAAATTCATTTGTGGGGGGTCCATT
    TCATTAAGAGCTGAATTTTCCCCCAAAATTATGTTTTAAAAAGCTTTTTTTGAGATAATTGTAGATTGAC
    ATGCACATACAAGAAAGGGTACAGAGAATTCCATACCCTTCACCCAGTTTCCCCTAACAAAGGTTACTTT
    TATATTTATTTATTTATTTTTAGAGACAGAGCATTCTTCTGTTGCCCAGGCTGGAGTGCAGTGGTGTGAT
    CTGGGCCCACTGCAACCTCCGCCTCCTGGATTCAAGTGAGTCTCTTGCTTCAGCCTCCCTAGTAGCTGGG
    ATTACAGGCACCCGCCATCATGTCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGATTTCACCATGTTA
    GCCAGGCTGGTCTCAAACTCCTGAACTCTAGTAATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTAC
    AGGTGTGAGCCACCATGCCTGGCCAAAGGTTACTTTTAATGTTCATTAATTTTGAAGACTTGCAGTTTAT
    TTATGCTATAAAGGACAATTGTGCACTATTAATAGTCGCAATGATGACATAATCTAAGACTTTTAATATT
    TAGAAACTTATGGTTGCAGGAAATTAAAAATTCATCTCATGTGCTTTTTTGGCAGAGAAGTATGAAATAT
    TGATCAATATAAGGTCCTTAAGCATATGCACCTATTAAACTTTTATGGAGGAAGTAGAATAGAATTCATA
    AGTCCATTTGTTTTTACTCTTTATTTTTTTGAGACAGAGTCTCACTCTGCCGCCCAGGCTGGACCGCAGT
    GGCACAATCTCCACTCACTGCAACCTCTGCCTCCTTGGTTCAAGTGATTCTCGTGTCTCAGCCACCTGAG
    TAGCTGGGATTGCTACTCAGGTGTGCACCACCACACCCGACTAATTTTTGTATTTTTAGTAGAGATGGGA
    TTTCACCCCTTTGGCCAGGTTGGTCTTGAACTCTTGGCCTCAAGTGATCTGCCTGCCTTGGCTTCCCAAA
    ATGCTGGGATTACAGGTGTGAACCATCGCGCCTGGCCTAGAATTCATAAGTTTAAGGAGAAAAAGGAGTG
    ATGAAAAATTTCCAAAAGTTAAGAACTTTTTCTTGTATTTTAAAAATGAGTGACGGTTGAAACAAAATTG
    ATATATTATTTATGTGCTATTAGAAATAATTAAAAAGAATAAGGTTTTCTTTTTTAAAAATCAACATTTA
    CTTTGGAAATTATGTCCTTTATAGTGATGAAAAATGTTTGATGTCAAGTTAAAAGTGAGTAAAGAAGTAC
    ATTCATTTTATAACAGCATTTTTCACAGTAGCTAAAATGTGGTAGCAACCCAAGTGTTTATTACTGGATG
    AATAGATAAACAAAATGTGGACTCAGGCATCTCTCTTCTGGGCTCATACCCAAAGGAAATGAAATCACCA
    CCTCACCAAGGTATCTGTACTCCTGTGTTTATTGTAGCATTATTCATAATAACCAAGATATGGAGACAAC
    CTAGACAACCATCAATGGACGAATGGTTTAAGAAATTGTGGTACGGATATATACAAGGGAATTCAGCCTT
    AAAAAAGGAGGAGATAGCATTTGCCATAACCTAGACGGGCCTGGAGGACATTATGCTAAATGAAAGAAGC
    CAGACACAGAAAAAAAATATTGCACGATCTTACCTGTATGTGGAATCTTTTATTTATTTATTTTCTTAGA
    CGGAGTCTCACTCTGTTGCCCACGCTGGAGTGCAGTGGCGCAATTTTGGCTCACCGCAACCTCTGCCTCC
    TGGGTTCAAGTAATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCACCTGCCACCACACCTG
    GCTAATTGTATTTTTAGTAGATATGGGGTTTTGCCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCA
    GATGATCCACCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCTACCATGCCTGGCCTGTGG
    AATCTTTTAAAAAGGTCAAATATATAGAGAATAAAACAGTGGTTATCAAGATTGGAGTAGGAGAGAGGAA
    ATGGGCAGATGTAGGCCTAAGGATACGAAGTAGCAAACATATAGGATGAACAAGTCAAATAAAGTACAAT
    ATGAAGACTACAATTAATAATAGCATATTATTCCAGGATTTTTGCTAGATGAGCATAGTTGCTTTTGCTA
    CAGGAGGAAATAAATGGGTAACTAAGTGAGATGATGCATAAGTCAATTTGTTTCACTATAGTATCCATTC
    TACTAAATATGTGTGTCTTATACCATGATGTTGTATATACCTTATATATACACAATAAAATTTATTTAAA
    CAAACAAAATGTGGTATATACATAGAGTGGAATATTATTCAGCCTTAAAAAGGAAGTTCTGACACATGCC
    ACAATATGGATGAATCTTGAGGATATTATGCTAAGTGAATTAAGCCAGTCACAAAAAACCAAATACTGTA
    TGATTCTATGTATTTGAGGTATTTAAAGTAGTCAAAATAATAGAGACAGAAAGTAGAATGGTGGTTGGGA
    CAGGCTGGGGAGAGGGAGGAGTGGGAGTTATGTTTAACGGTTATAGAGTTTTAGTTTTTCTAGATGAAAA
    GAGTTAGAGAGAAGGATGGTGGTGAAGGTTGCACAACAATGTGCATGAACTTAATACCACTGAACTGTAC
    ACTTAAATGTTAAAATGGTAAGATTGTTATGTGTATTTTGCCACAATAAATTAGTATTATTATTTTTTTT
    AGAGACAGAGTCTAGCTCTGTCAACCAGGTTGAGTGCATTGGTGTGATAACAGCTCACTGCAGCCTCCAA
    CTCTTGAGCCCCCAACTCCTGGCCTCAAGCAGTCCTCCTGCCTCAGCCTCCCAAAGTGCTGAGATTACAG
    GGGTGAGCCATTCACTGTTCCTTGCTAAATCTTTTTTTTTTTTTTTTTTTTTTTTTTGAGACAGAGTCTC
    GCTCTGTCACCCAGGCTGGAGTGCAGTGGTGCGATCTCAGCTCGCTGCAAGCTCCACCTCCTGGGCTTAA
    ATGATTTTCCTACTTCAGCTTCCTGAGTAGCTGGGATTACAGGCACACGCCACCATGCCTGGCTAATTTT
    TGTATTTGTAGTAGAGACAGAGTTTTGCCATGTTGGCCAGACTGGTCTCGAACTCCTGACCTCAAGTGAT
    CCACCTGCCTAAGCCTTCCTAAGTGTTGGGATTACAGGCATGAGCCACCACACCTGGCCTAAATTTTTTT
    TTTTAATGAGTGAAGGAGTCCATAGTCTTTCATACTTTGGGGTGATACAAGAAAAGGAAAAAGGTTTAAT
    GACAACTGCCACTTTCATTTATTCATTCCTTTGTTGATGTACTTTTGGGCTGGGTTTTGCTGTTATAAGC
    TAAACTACAATGGACATCCTTGTACACATTCCCTAGAGTCCAAGTACAAGATTTTCTCTAGGGTGTTTTC
    CTGGAAAAGGAATGACTTGAGGAGTATGGCAACCTTGTGAGAAATGACTGATTCGAAAGATGTGTCCCAT
    AGCTTTTGATACATTTTTCTCCGAAGTCATGGTTCCAGTCCACACTCACACCAGCTGTGGAGGAGTTTTC
    ATTTACCCACGAGCTTTGTTATTTCTGGTTTTGTCAGTCTTTTCAATTCTGCCAATCTCATAGGGTGTGA
    TGGTATCTCGTTGTTTAAACCTGACTTCTCTTGTTAAGGTTGAGACCGAGCATCTGGCCCCATGTTTGCT
    GGCTATTCAGCTTTCCTCTTCTGCAGACTGCCCGTTTGTCCACTGCTCTGTTTCAGTAACTTCCATATAC
    CAAGAACTCTCTGCCTTCCATAACTCACATCACTTAAAATACGAATTTTTTTGATGCTTAGAGAACCTGA
    TTTCCTTCCAACCAATTGTGCTCTCCTAATTCCAACAACCAAATGAAGCTTCAACTCTTCTTTTTGTTCA
    GAGCCCTCCTGAGTCCTGTCCTACTCACAGTAAAGGCTGTGGCAAAATAAATAAATAAGTAAATAAAGGG
    GAAAAATACACTGCATCCAAAAAAGGCTCGTTTCTTTTTCTTTCTTTCTCTCTCTCTCTTTCTTTTTCTT
    TTTCTCTTGTTGTGACAATTGTCACAACAAAATGTGTATGTGACAATTTGTTATCTTAAAACAGTTTACC
    CAAAATAGAAACCTTACAAAGCCAGTGGAAACTTTTCTTTTTGCATAAGTTGGTTAATTCTTTTGAGTGC
    TGGCCTGGGATCCATGAAATAACCTCAACTCGACCATAAAACTTTTCACTCCCACGTTATGCAAACGGTG
    ATGACCTTGTTGGTGGCAAGCCCCCCGTGTGACTTTCCGAGGGGATCTGAGTGGTGCAAGGTGGAGGACG
    CAGTGATGGAAACATTGCGAGAGGGAAGGCGTCTGTTTCCACCCACTTACCCTCAGAGCATGAGGGCTGG
    GCGAAGGCTCTGACTCCTGTAGGGGGTGACCCATTTCTAGGACTATAGAAGGAGAGGTGTGGACCTGGAA
    AAAGGAAGGAAAGACCACGGCAAGGAAAGTCAAGAAGTGGGAGAAGTCAAGGGCTCTCTCCTCCCTTCCT
    CCTTGTCCTTGGCCTGCAGCAACTCCTCTTTCTCTTTTTGTGGGGACAGACTGAAGAGGATTTCTTAGTA
    TGGTTTGTACCTTCCCAGTAGAGCAGGAAAAAGAGGAACGAGCTGCTCCCTCCACACCTCAGACGCTGAG
    GTCAACTGCCCTTTGGGCGGGGCACGTTGGCTCACGCATCTAATCCCAGCACTTTGGGAGGCCGTGAGGG
    GAGCATCACTTGAGCCCAGGAGTTGGAGACCGGCCTGGGCCACATAGTGAGGCCCCATCCTCCGGCTTGC
    ACCACTCAAATTCCCTCGTAAAGTCACACGGGGAGCTTGCCCCCAGCAAGGTCGTCAGTTTGCAAAACAT
    GGGAGTGAAAAGCTTTATGGTGGAGTGTAGGTTATTTCATGGATCCCAGGCCAGCACCCAAAAGAATTAA
    CCATCTTGTGCAAAAAGAAAATTTTCCACTACTTTTTTAAAGTTTCTATTTTGGGTAAATCGTTTCAAGA
    TAACAAATTGGAGAGAGACCTAGAGAGAAATGAGCCTTTTTAAGATGCAGTTTATTTTGCCCCATGATGT
    TTTTGTTTTTGTTTTTGCCAGAGCCTTTACTGTGAGTAGGACAGGACTCAGGAGGCCTCTGAACAAAAAG
    AAGCGTTGAAGCTTCATTTGGTTGTTGGAATTGGGAGAGCACAATTGGTTGGAAGGAAATCAGGTTCTCT
    CAAAAAATAAAATAAAATAAAATAAAAAAAAAAGCCAGCGTGCTGGCGCGGGTCCATGGTCCAAGCTACT
    TGGGAGGCTGAGGTGGGGGGATCGCTTGAGCCCGGAAGTCGAGGCTGTAGTGAGCCGTGATTGCACCACT
    GCACTCCAGCCTGGGCGATAGAACAAGACCCTGTCTCTTAAAACAAACAAGAAACAAAACAAAACAAACA
    ACAAAAAAATTCATTTGGGAATGTTTCTGCGCGTGCCCATAAGCAGAGCCATGCTATAGGATCTCCCCTG
    TGCCCCAACAATCAGCTTCTTACTTGGAGGGTAGAGAACGGTGCTTCCCCACGCTGCTGTGCAATGGATA
    GGAGCTATGTGAAGCTGGCGTATGGGTGGGGTTTGCAGGCTTCGTCCGGCTTCATCGCCGGCTGCTGACC
    CGGCACCAATTCCTGTTCTGCAGGTCTCACCGCAGAGGGGCACGCCAGCCATGAGGACAGATGAGGGAAC
    ACGTGATGCCACGATGGGGGTGCCAGGATGAGGTGGGTGCGGTCGCGGACAGGCGCACGAGGAGCCCAGC
    GGAGCGCCACCCGGAGCAGGCGCGGAGGAGGGCTGGGGAGGGCCACCAAGGCGACGAGAGCCGGTGTGCC
    TGAATCAGCCTAAAGGAGACGGAGGAGGAGTGTGGTGGGCGCAGGGGCAGGGAGCTGGGGGAAGGGCGGG
    GGGCTAGCCCAGGCTGAAGGCAGGCAGGAGCAGGGCCGCGATGTCAGACAAGAAACGGCAGCAGTGTGTT
    TGGGAACCTAGCACCAACCGCACCGGTGGAGACAGGGTCTGCTGTAGAGGAGGTGGGGCCGGGTCCCAGC
    TAAGTAAGGCGGTGGATCTTGCAGCCCTTCCATCCTCAGCCGCTCATTCTGCGCAAATCTCGGGGCCAGC
    CTTGGTGGAGCCGTAAAGCGTCCACCAGAACCTGGATCCCTCCGCCACCTTTCCCATGAATTCACCTTTC
    TGTACACAGCAAGCGCCTGAGCGGAGACGGCCGACACGTTTCCCACTGTTACCCCAGGAAACCGCGGCTC
    CTGAGGGGGTCAGGGCCTGGGCAGGGGGCAGAGCTCAGCGCGCCGTACTGAGGCAGAAACGGGGTCCAGA
    GAGGGTGGGGTGGGGGTACAGGGAAGGGTCGCCCGAAGGTCCCTGGCGCAGGGAGGACAGAGGAGGGATC
    TAGAATTCGTAGGGGAGAAGAGAACTCAGAAAAGATCCGGCCCAGCGCATTTATTTTACAGA
  • Protein Sequence : Show Sequence
    >gi|225007536|ref|NP_001139345.1| T-cell surface glycoprotein CD8 alpha chain isoform 1 precursor [Homo sapiens]
    MALPVTALLLPLALLLHAARPSQFRVSPLDRTWNLGETVELKCQVLLSNPTSGCSWLFQPRGAAASPTFL
    LYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGYYFCSALSNSIMYFSHFVPVFLPAKPTTT
    PAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCNHRN
    RRRVCKCPRPVVKSGDKPSLSARYV
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Recombinant Aeromonas hydrophila vaccine (Aera)
2. IFN1
  • Gene Name : IFN1
  • Sequence Strain (Species/Organism) : Ovis aries
  • NCBI Gene ID : 492823
  • Genbank Accession : AMGL01050517
  • Taxonomy ID : 9940
  • Chromosome No : 2
  • Gene Starting Position : 89172785
  • Gene Ending Position : 89173443
  • Gene Strand (Orientation) : +
  • Protein Name : transcript variant X1
  • DNA Sequence : Show Sequence
    >gi|417531962:89172785-89173443 Ovis aries breed Texel chromosome 2, Oar_v3.1, whole genome shotgun sequence
    TAGGAAAAAAAAATCTGAAAAGGCTCTGTGGGCAGAAGAAATAAGGGTAACATTAAAAAAAAAAAAATGG
    TTGAGAAATTTTGCCCTAACCCATTTGGAGAGTACAAAATGAAAAGCAAAGAGAAAAGTGGAAAGTAAGA
    GGGAACTTTCAGAAAATGGAACCCATGGGCTCCTATTTAAGACACAGGCCTGAAGGAAGGGCTTCAGAGA
    ACCTAGAAAGCAGGTTCACAGTCACACACCACCCCAGGCCTGAAGCATCTGCAAGGCCCCAATGGCCGCA
    GCCTGGTCCTTGCTCCTGGCCCTGCTGCTGCTCAGCTGCAACGCCATCTGCTCTTGGGGCTGCCACCTGC
    CTCACACCCACAGCCTGGCCAACAGGAGGGTCCTGACACTCCTGCAACAACTGAGGAGGGTCTCCTCTTC
    CTCCTGCCTGCAGGACAGAAAAGACTTCGCATTCCCCCAGAAGGCGCTGGGCGGCAGCCAGTTGCAGAAG
    GCTCAAGCCATCTCTGTGCTCCACGAGGTGACCCAGCACACCTTCCAGCTCTTCAGCACAGAGGGCTCGG
    CCGCCGCGTGGGACCAGAGACTCCTGGACAAGCTCCGCGCTGCACTGGATCAGCAGCTCACTGACCTGCA
    AGCCTGTCTCAGGCAGGAGGAGGGGCTGC
  • Molecule Role : Protective antigen
  • Related Vaccine(s): Recombinant Aeromonas hydrophila vaccine (Aera)
3. IgM
  • Gene Name : IgM
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16019
  • Genbank Accession : AC073553
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 171229
  • Gene Ending Position : 175133
  • Gene Strand (Orientation) : -
  • Protein Name : immunoglobulin heavy constant mu
  • Protein Note : Also known as Igm; muH; Igh6; Igh-6; Igh-M; AI326478; MGC107036; MGC107507
  • DNA Sequence : Show Sequence
    >gi|121699722:171229-175133 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
    GTCATTTCACCTGCAGGTGACAGAGACAGTGTGAATTGCTGTCCACTTGTAGGGAGTAGAGGTAGAGGCA
    GTGAGGACTGGTGACTCCCTGTCCCAGCCCTGTGTCCTCAGCCCCACAACCACACTACCTTGAACAGGGT
    GACGGTGGTGCTGTAGAAGAGGCTCAGGAGGAAGAGGACGATGAAGGTGGAGGCAGTGGTCCACAGGTTC
    TCAAAGCCTTCCTCCTCAGCATTCACCTCCCCCTCTATGAAGGGACACAGAGAGGGAGGGTCAAGCCAAG
    TCTTGGGGTGACTGAGCATTTGCATAAGGGTTGGGGTGCCCTGATGGTTCTGGAGAATCTTAGCGAGTAT
    TGACTGGTCCCCTAATAGCTCAAGCTAGACGGAGGCACAGAACAGGCCAAGCTGTAGGACACACTAATTT
    GGAGCCTTTGTTCCAGGAGCCCTGGGGCAGCAGAGCAAGCAAGACAGATGCAATGTATGGACCTGGGTAA
    AGGCAGACTCAGGAACTGTGGCAAAATGATATTGGTGTGCTCTGGACTTTCCCTGAGCAGGTGGTGCCAT
    CGGTGCTGTTTAGACTGAGAATTTAGAGCAGGTCTCCCTGTCTCTTGGGCTTTAGCAATAAGAACAGGGA
    TACCAGAGGTTGTCCCTCCTTACTTCCTGAAGGACTCAGGCATCTCATGACTTTCTTTGATCAGAGTTCC
    CGTGCGTTGCTTGTCCTACCCCTGCCAGCAATGTTGGGTCTTGGTACCAAGAGATAAACAGACAGATACC
    TGAGGGAGGCTGTGGGACCAGGGCATTTATTGGGATCTCTGATCCCTGTAAAGATGAGCCCTTGGGGATA
    TCTAAAGCCTAGGCCCCAAATGGATATGAATATGAAGAATGTCCAGGATATGAGCCCAAAGAGGCAGTGT
    TATTGGGTGGTGACTCCTTACAGAGGCTCCTTGATGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
    GTGTGTGTGTGTGTGTGTGTGTGTGTGGTTGGGGCATCTTTTGGACAACTGCATTATGTTTTCTGCATTT
    TGAACTGGAGATCAGGGTGGGTGTGAGTGGGTTAGGCAATTTCACATTGGTCTTCTGAGGTGTTCTATTA
    ATGGAGACACAGCCAAGGCTAACTTTCTGTCATGGGTGGTTAGTGGAGTATCTTCTGCCCCATGGTGTTG
    AGACAGATAGGCATACTTTGTACCTGGGGTTATCTCTCCCAATCATAGAGTGGGCTCCTGAGGAGTATCA
    GTGACCCAGCCTAGTGTAGGCTCTCTGAGCCGTGAGCCTTGGAGATTACAGCCCTGGTCAGAGTCCAGGT
    GTATCTGTGTGCTATACCTGTTCACATGTTAGTGTACACATGTGAAGATGTTTCTGTACATGTGTGTATA
    TGCATATCTTTGTATCTATATAGTTTATTTGTGTATATTTGCCTATAGTCTGTTTCTGTGTACACAGGAC
    CATGCATATTGGTATCTGTTTCTGTGAGTGGTTGTGTGTCTATGCCTATTTGTCTCTGAAGGCGTTTGTA
    AGTGTCCATGCATGTGTGTGCGCATGAACTCATGTGTATATATGTGTACCTTTGTCTGTGTGTCCATGAA
    TACACATTCATGTATGTGTCTACACATGTGTACCTATATATCTATGTATAAATGCATGTGTGTCTATATA
    TGTGCCTGAATGCTGCCTGCACCAGGTTAGGCAAGCTTTCAGCCATATATCTAGAAGTAGCTGGAGGAAT
    CGCATGTTCATACTCTGTCTGTTGTAAGCCTGACTGAGTTCACACACAAGGAGGAAACTGGCAAGGTAGA
    GGAAGGGCCAGGGTGGAGGCAAGTATGCAGGGTGTGTGCAGAGCAAGCAAAACACAACTGTCTCAGCAGG
    CATGAGCATTGTATAATCAAAACCAGTGACGTTTGAATGGATTTTTTTTATTTCTAATTTTTATAAAATG
    CAACATCTCACTCTGACATGGTTAGTTTGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGA
    GCGCTAGCATGGTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTACAGTGTGGGT
    TTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGTGGCAGGGCCTCGTGGCCTACAACACAGG
    TATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGCTGTGGGTAAAGTAGAAGCCTGG
    GGCCCCAGGCTCTGGCATCGGGGCACTGGTCACATACTTCTCTTGGGGCAAGAGTTGCCCTCTCTGAAGC
    CACTGCACACTGATGTCTGCAGGAGAGAAGCCCTTCACCAGGCAGGTGACTGTGGCTGACTCCCTCAGGT
    TCAGTTGCTCACGAGCTGGTGGCAGCAGGTACACAGCAGGTGGATGTTTGTGCACCTCTGTAAATGAAGA
    CAGTAGTGAGGATAGGGTGGAAGAGGACCTGCCCTCCCTATGAGGTACAGGAAGGGTCCTGCAATTGGAG
    GGGAAGGGAAGGGGGGATACCTACCATTGGGTTTTGAGATGAATTTCTTCTGTGGTGAAGGCAGATCCCT
    GTGAGTCACAGTACACACAAATTCCTTCCTGTTATTCCAGTCTTCCACACAAACACTAGCCACACCCTTA
    GCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTTGGTTTCCAGTGGTTCACCACTTTGAG
    AAGCCCAGGAGATATTCAGGGTTTCATAGGTTGCCAGGTTTGAGACCAGACAGGTCAGGTTAGCGGACTT
    GCTGAGGAAGATGTCGGCAAAGGAGGGGGGGATGGTGAAGGTTAGGATGTCTGTGGAGGGACCTGCAGTC
    AAGAGAACACTGTGTCAGGTTATGACATATCTAGTTGTGAAGTTGGAAAAAAGCTTAGTCTCCATCCCCC
    AAACCTACTTAAGGGAAGATGCTGGTCCTTCCAGCTGATTCTCACCCCTCTACTGCCTATTGCTCATTCT
    TGTCCTTGGCCACTCTGGGGAGCCAAAGTTCAAGGAGCAAATGACCATGTCTGGGTGGCATTGGCCATAA
    TTGTAGGAGGACTTCCCTAATCTGGGAGGGCTAGGCATTGGGCTTAGCCCAGGCCACTCACTGGCAGCAC
    ATGTGGAGGACACGTTCTTCAAGAAGGTGAGACCCCTGTGATCCACACGGCAGGTGTACACATTCAGGTT
    CAGCCAGTCGATTTCAGAGATGGTAAGTGTGCTTATGACCTTGTAGGTTTGGGGTGTGGATCCTTTGTTC
    TCGATGGTCACCGGATCTGTGGTGAAGCCAGATTCCACGAGCTTCCCATCCTTTAGCCAGGATACTGTGA
    TCGGTTTTGGAGTGAAGTTCGTGGCCTCGCAGATGAGTTTAGACTTGCGTGGTGCAGGGCCAGAGAAGCC
    ATCCCGTGGTGGGACGAACACATTTACATTGGGGTTCATCTCTGCGACAGCTGGAATGAAAGGTCAAGGT
    GTGAGCTCAGCTTGGCCTAAGCCCCACCCCGCTGCTCCCTCTGGGCCATGCCTGGGCCTGGGCACCCCTG
    CTGGGAGGGTTTGGTTCTTACCTGGAATGGGCACATGCAGATCTTTGTTTTTGCCTCCGTAGTGGATTTT
    GCATACCAGGTATTCATCTGAACCTTCAAGGATGCTCTTGGGAGACAGCAACACCTGCGAGGTGGCTAGG
    TACTTGCCCCCTGTCCTCAGTGTTGGGAAGGTTCTGATACCCTGGATGACTTCAGTGTTGTTCTGGTAGT
    TCCAGGTGAAGGAAATGGTGCTGGGCAGGAAGTCCCGGGCCAGGCAGCCCATGGCCACCAGATTCTTATC
    AGACAGGGGGCTCTCGCAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTC
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Recombinant Aeromonas hydrophila vaccine (Aera)
4. IL8
  • Gene Name : IL8
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 3576
  • NCBI Protein GI : 10834978
  • Genbank Accession : ABBA01022993
  • Protein Accession : NP_000575
  • Taxonomy ID : 9606
  • Chromosome No : 4
  • Gene Starting Position : 74606222
  • Gene Ending Position : 74609432
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 8
  • Protein pI : 9.09
  • Protein Weight : 10403.36
  • Protein Length : 99
  • Protein Note : Also known as NAF; GCP1; LECT; LUCT; NAP1; CXCL8; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
  • DNA Sequence : Show Sequence
    >gi|224589816:74606222-74609432 Homo sapiens chromosome 4, GRCh37.p10 Primary Assembly
    TGAGGGTGCATAAGTTCTCTAGTAGGGTGATGATATAAAAAGCCACCGGAGCACTCCATAAGGCACAAAC
    TTTCAGAGACAGCAGAGCACACAAGCTTCTAGGACAAGAGCCAGGAAGAAACCACCGGAAGGAACCATCT
    CACTGTGTGTAAACATGACTTCCAAGCTGGCCGTGGCTCTCTTGGCAGCCTTCCTGATTTCTGCAGCTCT
    GTGTGAAGGTAAGCACATCTTTCTGACCTACAGCGTTTTCCTATGTCTAAATGTGATCCTTAGATAGCAA
    AGCTATTCTTGATGCTTTGGTAACAAACATCCTTTTTATTCAGAAACAGAATATAATCTTAGCAGTCAAT
    TAATGTTAAATTGAAGATTTAGAAAAAACTATATATAACACTTAGGAAAGTATAAAGTTTGATCAATATA
    GATATTCTGCTTTTATAATTTATACCATGTAGCATGCATATATTTAACGTAAATAAGTAATTTATAGTAT
    GTCCTATTGAGAACCACGGTTACCTATATTATGTATTAATATTGAGTTGAGCAAGGTAACTCAGACAATT
    CCACTCCTTGTAGTATTTCATTGACAAGCCTCAGATTTGTCATTAATTCCTGTCTGGTTTAAAGATACCC
    TGATTATAGACCAGGCATGTATAACTTATTTATATATTTCTGTTAATTCTTTCTGAAGGCAATTTCTATG
    CTGGAGAGTCTTAGCTTGCCTACTATAAATAACACTGTGGTATCACAGAGGATTATGCAATATTGACCAG
    ATAAAAATACCATGAAGATGTTGATATTGTACAAAAAGAACTCTAACTCTTTATATAGGAAGTCGTTCAA
    TGTTGTCAGTTATGACTGTTTTTTAAAACAAAGAACTAACTGAGGTCAAGGGCTAGGAGAATATTCAGGA
    ATGAGTTCACTAGAAACATGATGCCTTCCATAGTCTCCAAATAATCATATTGGAATTAGAAAGGAAGTAG
    CTGGCAGAGCTGTGCCTGTTGATAAAATCAATCCTTAATCACTTTTTCCCCCAACAGGTGCAGTTTTGCC
    AAGGAGTGCTAAAGAACTTAGATGTCAGTGCATAAAGACATACTCCAAACCTTTCCACCCCAAATTTATC
    AAAGAACTGAGAGTGATTGAGAGTGGACCACACTGCGCCAACACAGAAATTATGTAAGTACTTTAAAAAA
    GATTAGATATTTTGTTTTAGCAAACTTAAAATTAAGGAAGGTGGAAATATTTAGGAAAGTTCCAGGTGTT
    AGGATTACAGTAGTAAATGAAACAAAACAAAATAAAAATATTTGTCTACATGACATTTAAATATGGTAGC
    TTCCACAACTACTATAAATGTTATTTTGGACTTAGACTTTATGCCTGACTTAAGGAATCATGATTTGAAT
    GCAAAAACTAAATATTAATCTGAACCATTTCTTTCTTATTTCAGTGTAAAGCTTTCTGATGGAAGAGAGC
    TCTGTCTGGACCCCAAGGAAAACTGGGTGCAGAGGGTTGTGGAGAAGTTTTTGAAGAGGTAAGTTATATA
    TTTTTTAATTTAAATTTTTCATTTATCCTGAGACATATAATCCAAAGTCAGCCTATAAATTTCTTTCTGT
    TGCTAAAAATCGTCATTAGGTATCTGCCTTTTTGGTTAAAAAAAAAAGGAATAGCATCAATAGTGAGTTT
    GTTGTACTCATGACCAGAAAGACCATACATAGTTTGCCCAGGAAATTCTGGGTTTAAGCTTGTGTCCTAT
    ACTCTTAGTAAAGTTCTTTGTCACTCCCAGTAGTGTCCTATTTTAGATGATAATTTCTTTGATCTCCCTA
    TTTATAGTTGAGAATATAGAGCATTTCTAACACATGAATGTCAAAGACTATATTGACTTTTCAAGAACCC
    TACTTTCCTTCTTATTAAACATAGCTCATCTTTATATTTTTAATTTTATTTTAGGGCTGAGAATTCATAA
    AAAAATTCATTCTCTGTGGTATCCAAGAATCAGTGAAGATGCCAGTGAAACTTCAAGCAAATCTACTTCA
    ACACTTCATGTATTGTGTGGGTCTGTTGTAGGGTTGCCAGATGCAATACAAGATTCCTGGTTAAATTTGA
    ATTTCAGTAAACAATGAATAGTTTTTCATTGTACCATGAAATATCCAGAACATACTTATATGTAAAGTAT
    TATTTATTTGAATCTACAAAAAACAACAAATAATTTTTAAATATAAGGATTTTCCTAGATATTGCACGGG
    AGAATATACAAATAGCAAAATTGAGGCCAAGGGCCAAGAGAATATCCGAACTTTAATTTCAGGAATTGAA
    TGGGTTTGCTAGAATGTGATATTTGAAGCATCACATAAAAATGATGGGACAATAAATTTTGCCATAAAGT
    CAAATTTAGCTGGAAATCCTGGATTTTTTTCTGTTAAATCTGGCAACCCTAGTCTGCTAGCCAGGATCCA
    CAAGTCCTTGTTCCACTGTGCCTTGGTTTCTCCTTTATTTCTAAGTGGAAAAAGTATTAGCCACCATCTT
    ACCTCACAGTGATGTTGTGAGGACATGTGGAAGCACTTTAAGTTTTTTCATCATAACATAAATTATTTTC
    AAGTGTAACTTATTAACCTATTTATTATTTATGTATTTATTTAAGCATCAAATATTTGTGCAAGAATTTG
    GAAAAATAGAAGATGAATCATTGATTGAATAGTTATAAAGATGTTATAGTAAATTTATTTTATTTTAGAT
    ATTAAATGATGTTTTATTAGATAAATTTCAATCAGGGTTTTTAGATTAAACAAACAAACAATTGGGTACC
    CAGTTAAATTTTCATTTCAGATAAACAACAAATAATTTTTTAGTATAAGTACATTATTGTTTATCTGAAA
    TTTTAATTGAACTAACAATCCTAGTTTGATACTCCCAGTCTTGTCATTGCCAGCTGTGTTGGTAGTGCTG
    TGTTGAATTACGGAATAATGAGTTAGAACTATTAAAACAGCCAAAACTCCACAGTCAATATTAGTAATTT
    CTTGCTGGTTGAAACTTGTTTATTATGTACAAATAGATTCTTATAATATTATTTAAATGACTGCATTTTT
    AAATACAAGGCTTTATATTTTTAACTTTAAGATGTTTTTATGTGCTCTCCAAATTTTTTTTACTGTTTCT
    GATTGTATGGAAATATAAAAGTAAATATGAAACATTTAAAATATAATTTGTTGTCAAAGTA
  • Protein Sequence : Show Sequence
    >gi|10834978|ref|NP_000575.1| interleukin-8 precursor [Homo sapiens]
    MTSKLAVALLAAFLISAALCEGAVLPRSAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKL
    SDGRELCLDPKENWVQRVVEKFLKRAENS
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Recombinant Aeromonas hydrophila vaccine (Aera)
5. TNF-alpha
  • Gene Name : TNF-alpha
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 21926
  • NCBI Protein GI : 7305585
  • Genbank Accession : AB039224
  • Protein Accession : NP_038721
  • Taxonomy ID : 10090
  • Chromosome No : 17
  • Gene Starting Position : 35199389
  • Gene Ending Position : 35201995
  • Gene Strand (Orientation) : -
  • Protein Name : tumor necrosis factor
  • Protein pI : 4.75
  • Protein Weight : 24010.47
  • Protein Length : 235
  • Protein Note : Also known as DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434
  • DNA Sequence : Show Sequence
    >gi|372099093:35199389-35201995 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J
    CTTTATTTCTCTCAATGACCCGTAGGGCGATTACAGTCACGGCTCCCGTGGGGAGCAGAGGTTCAGTGAT
    GTAGCGACAGCCTGGTCACCAAATCAGCGTTATTAAGACAATTGGGTTAGATAAATATTTTGTTTTAAAC
    ATAAGCAAAAGAGGAGGCAACAAGGTAGAGAGGCCAGGTGGGGACAGCTCAGCTCCGTTTTCACAGAAAA
    CATGTCTGTCTGAAGACAGCTTCCCACACTGGGTCCTCCAGGACACCCCGGCCTTCCAAATAAATACATT
    CATAAGCAAATAAATAAATAATAAATAAATAATAAATAATAAGTGCAAATATAAATAGAGGGGGGCTGGC
    TCTGTGAGGAAGGCTGTGCATTGCACCTCAGGGAAGAATCTGGAAAGGTCTGAAGGTAGGAAGGCCTGAG
    ATCTTATCCAGCCTCATTCTGAGACAGAGGCAACCTGACCACTCTCCCTTTGCAGAACTCAGGAATGGAC
    ATTCGAGGCTCCAGTGAATTCGGAAAGCCCATTTGAGTCCTTGATGGTGGTGCATGAGAGGCCCACAGTC
    CAGGTCACTGTCCCAGCATCTTGTGTTTCTGAGTAGTTGTTGAAAGCTCTGAGCACAGAGTTGGACCCTG
    AGCCATAATCCCCTTTCTAAGTTAGAAGGATACAGACTGGGGGCTCTGAGGAGTAGACAATAAAGGGGTC
    AGAGTAAAGGGGTCAGAGTGGGGGCTGGGTAGAGAATGGATGAACACCCATTCCCTTCACAGAGCAATGA
    CTCCAAAGTAGACCTGCCCGGACTCCGCAAAGTCTAAGTACTTGGGCAGATTGACCTCAGCGCTGAGTTG
    GTCCCCCTTCTCCAGCTGGAAGACTCCTCCCAGGTATATGGGCTCATACCAGGGTTTGAGCTCAGCCCCC
    TCAGGGGTGTCCTTGGGGCAGGGGCTCTTGACGGCAGAGAGGAGGTTGACTTTCTCCTGGTATGAGATAG
    CAAATCGGCTGACGGTGTGGGTGAGGAGCACGTAGTCGGGGCAGCCTTGTCCCTTGAAGAGAACCTGGGA
    GTAGACAAGGTACAACCCATCGGCTGGCACCACTAGTTGGTTGTCTTTGAGATCCATGCCGTTGGCCAGG
    AGGGCGTTGGCGCGCTGGCTCAGCCACTCCAGCTGCTCCTCCACTTGGTGGTTTGCTGAGGGGGGGGGGG
    AGGATTGAGTCAGTGTCACCCTCTTAGTTCACACTCCACATCCTGAGCCTCAGCAGCTACCCACACTTCA
    CTTCCGGTTCCTGCACCCTCTGTCTTTCCACATCCCATTGGCTATGAGGTCCCGGGTGGCCCCCTGATGC
    CTTGCTTTTGAGTCACTGCTCTGACTCTCACGTGCTGTCTCTAAGAGCTCTGTCTTTTCTCAGCCTGGCT
    CGACACCCCTCAACCCGCCCCCCAAAATCATGCCCCTTCATTCTCAAGGCACATGTAAAGAAATCTTACC
    TACGACGTGGGCTACAGGCTTGTCACTCGAATTTTGAGAAGATGATCCTGGAGGGGAAGAGACAAAGGCA
    AGGATGAGCCTTTTAGGCTTCCCAGCAAGCATCTATGCACTTAGACCCCTTTCCTCCCAAACCAAAGCTT
    TAAGTTCTCCCCCCACCCCATCTCATCCCATGCCTAACTGCCCTTCCTCCATCTTAAATTAAGAGAGAGG
    TGTGGGAACACTTACTGAGTGTGAGGGTCTGGGCCATAGAACTGATGAGAGGGAGGCCATTTGGGAACTT
    CTGTGTAGGAAAAGGAGGTTAGTTAAGACAGACTCACCCCAAAGGAGAAGCCTCCCGGCTGATTGCCCCG
    CTTACAGTTCCTCTTTGCCCCACCCCACCCCCCAGCTTTGTGTTTTTCTTCTTCATTCATTCATCTGTCC
    AACCCACGGCTTCTTTCTGCGGTGCCCTCTGTGCTTGATCTCCCGTTATCTCCCCTTCATCTTCCTCCTT
    ATCTCTCATGCCTCTCTCATTTCTGTCTCTGAGTTTTATCTCTTGCTTATCCCCTCTTCCCCTGGCCACA
    TCTTTCCAGATCTCTCCACGTGTGAACACACTTGTTCGTTCATTCATCTCTCTGTGCATCCGACGAAGGA
    TGTTTAGTCAGCTGGACGCATGGGTCCGAGGTCCTGACTCTGTCCCCTCCACACTCTCCTCCACCTTGCC
    CTGCCCATTAGCCCACTTCTTTCCCTCACACTGTCCTTCTTGCCCTCCTAACCCGTTTTGCTTGTGAGCG
    AGAATAAGGGTTGCCCAGACACTCACCTCATCCCTTTGGGGACCGATCACCCCGAAGTTCAGTAGACAGA
    AGAGCGTGGTGGCCCCTGCCACAAGCAGGAATGAGAAGAGGCTGAGACATAGGCACCGCCTGGAGTTCTG
    GAAGCCCCCCATCTTTTGGGGGAGTGCCTCTTCTGCCAGTTCCACGTCGCGGATCATGCTTTCTGTGCTC
    ATGGTGTCTTTTCTGGAGGGAGATGTGGCGCCTTGGGCCAGTGAGTGAAAGGGACAGAACCTGCCTGGTT
    GGCTGCTTGCTTTTCTGGGAGCTATTTCCAAGATGTTCTGGAGTTTCTGTTCTCCCTCCTGGCTAGTCCC
    TTGCTGTCCTCGCTGAG
  • Protein Sequence : Show Sequence
    >gi|7305585|ref|NP_038721.1| tumor necrosis factor [Mus musculus]
    MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRDEKFPNGLPL
    ISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQ
    VLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQL
    SAEVNLPKYLDFAESGQVYFGVIAL
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Recombinant Aeromonas hydrophila vaccine (Aera)
IV. Vaccine Information
1. Aeromonas hydrophila aroA mutant vaccine
a. Vaccine Ontology ID:
VO_0002799
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Gene Engineering of aroA
  • Type: Gene mutation
  • Description:
  • Detailed Gene Information: Click here.
e. Immunization Route
Intraperitoneal injection (i.p.)
2. Aeromonas hydrophila LSA34 vector vaccine WED/pUTDgap
a. Vaccine Ontology ID:
VO_0004606
b. Type:
Recombinant vector vaccine
c. Status:
Research
d. Antigen
The protective antigen GAPDH from the fish pathogen A. hydrophila, encoded by the gene gapA34 (Mu et al., 2011).
e. Vector:
The Edwardsiella tarda strain EIB202 is used a a live, attenuated vaccine vector, using the promoter P(dps) as the protein-based expression system for the antigen (Mu et al., 2011).
f. Preparation
The gapA34 gene was introduced into P(dps) and transformed into live, attenuated E. tarda strain EIB202 (Mu et al., 2011).
g. Immunization Route
Intramuscular injection (i.m.)
h. Description
A. hydrophila is a common fish pathogen, so in order to create a vaccine against this pathogen, the gapA34 gene of A. hydrophila was expressed in the bacterial vector E. tarda strain EIB202, and it was shown to increase survival of vaccinated fish (Mu et al., 2011).
i. Fish Response
  • Host Strain: Turbot (Scophtalmus maximus)
  • Vaccine Immune Response Type: VO_0000287
  • Efficacy: Over 60% of the fish vaccinated with the WED/pUTDgap vector vaccine survived the A. hydrophila challenge (Mu et al., 2011).
  • Description: The P(dps) promoter--which was chosen due to its high transcription activity-- was shown to elicit survival in turbot, meaning that it has the potential to be an important part of bacterial vaccine vector application (Mu et al., 2011) .
3. Recombinant Aeromonas hydrophila vaccine (Aera)
a. Vaccine Ontology ID:
VO_0004811
b. Type:
Recombinant vector vaccine
c. Status:
Licensed
d. Host Species for Licensed Use:
Baboon
e. Immunization Route
Intramuscular injection (i.m.)
f. Fish Response
  • Vaccination Protocol: In vaccination experiments, healthy grass carp were distributed randomly into control group and bath immunization group (150 fish per group). For bath immunization, fish were immersed in 20 mg L−1 SWCNTs-Aera and the control group was immersed in PBS for 6 h. At the end of the vaccination, grass carp were transferred to different tanks without SWCNTs-Aera for 28 d. After 28 d, 15 fish in each replicate were euthanized in the laboratory through washrag soaked with 20.0 g/m3 MS-222. Sampled fish were dissected immediately with sterile scissors. The intestine, kidney and spleen were aseptically removed from their abdominal cavity, separately, where five fish were sampled for analyzing expression of seven immune-related genes through quantitative real-time PCR (qRT-PCR) and the experiment was performed in triplicate replicates. Thereafter, the intestine of three fish (15 fish per replicate) were also collected and pooled together as described elsewhere to extract microbial DNA for deep sequencing [29]. After 2 d, the remaining fish (15 grass carp in each replicate) were intramuscularly injected with 6.5 × 104 cfu mL−1A. hydrophila, kept for 10 days and thereafter euthanized for sampling. Subsequently, the intestine was carefully excised and maintained as above, which was also for extracting microbial DNA for deep sequencing. All the samples were placed into sterile polypropylene centrifuge tubes and stored in −80 °C until DNA extraction. All experiments were performed in triplicate (Liu et al., 2015).
  • Host Gene Response of CD8A
    • Gene Response: The expression of CD8A was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IFN1
    • Gene Response: The expression of IFN1 was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgM
    • Gene Response: The expression of IgM was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL8
    • Gene Response: The expression of IL8 was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
    • Detailed Gene Information: Click here.
  • Host Gene Response of TNF-alpha
    • Gene Response: The expression of TNF-A was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
    • Detailed Gene Information: Click here.
V. References
1. Hernanz et al., 1998: Hernanz Moral C, Flaño del Castillo E, López Fierro P, Villena Cortés A, Anguita Castillo J, Cascón Soriano A, Sánchez Salazar M, Razquín Peralta B, Naharro Carrasco G. Molecular characterization of the Aeromonas hydrophila aroA gene and potential use of an auxotrophic aroA mutant as a live attenuated vaccine. Infection and immunity. 1998; 66(5); 1813-1821. [PubMed: 9573055].
2. Liu et al., 2015: Liu L, Gong YX, Zhu B, Liu GL, Wang GX, Ling F. Effect of a new recombinant Aeromonas hydrophila vaccine on the grass carp intestinal microbiota and correlations with immunological responses. Fish & shellfish immunology. 2015; ; . [PubMed: 25862971].
3. Mu et al., 2011: Mu W, Guan L, Yan Y, Liu Q, Zhang Y. A novel in vivo inducible expression system in Edwardsiella tarda for potential application in bacterial polyvalence vaccine. Fish & shellfish immunology. 2011; 31(6); 1097-1105. [PubMed: 21964456].
4. Wiki: Aeromonas Hydrophila: Aeromonas Hydrophila [http://microbewiki.kenyon.edu/index.php/Aeromonas_Hydrophila]