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Human cytomegalovirus |
| Table of Contents |
- General Information
- NCBI Taxonomy ID
- Introduction
- Host Ranges and Animal Models
- Vaccine Related Pathogen Genes
- Glycoprotein B
(Protective antigen)
- IE2
(Protective antigen)
- pp65
(Protective antigen)
- UL123
(Protective antigen)
- UL83
(Protective antigen)
- Vaccine Information
- Human cytomegalovirus DNA vaccine pcGB
- Human cytomegalovirus DNA vaccine VCL-CB01 encoding phosphoprotein 65 and gB
- References
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| I. General Information |
| 1. NCBI Taxonomy ID: |
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10359 |
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2. Introduction |
Also known as human herpesvirus 5. Cytomegalovirus (CMV) is a common virus that can infect almost anyone. Most people don't know they have CMV because it rarely causes symptoms. However, if you're pregnant or have a weakened immune system, CMV is cause for concern.
Once infected with CMV, your body retains the virus for life. However, CMV usually remains dormant if you're healthy. CMV spreads through body fluids, such as blood, saliva, urine, semen and breast milk. People with weak immune systems have a greater risk of becoming ill from CMV. If you're pregnant and develop an active infection, you can pass the virus to your baby.
There's no cure for CMV, but drugs can help treat newborns and people with weak immune systems (Mayo Clinic - CMV). |
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3. Host Ranges and Animal Models |
| Humans |
| II. Vaccine Related Pathogen Genes |
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1. Glycoprotein B |
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Gene Name :
Glycoprotein B
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Sequence Strain (Species/Organism) :
Human betaherpesvirus 5
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NCBI Gene ID :
3077424
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NCBI Protein GI :
52139241
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Locus Tag :
HHV5wtgp060
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Genbank Accession :
AY446894
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Protein Accession :
YP_081514
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Taxonomy ID :
10359
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Gene Starting Position :
82065
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Gene Ending Position :
84788
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Gene Strand (Orientation) :
-
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Protein Name :
envelope glycoprotein B
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Protein pI :
7.31
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Protein Weight :
94542.1
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Protein Length :
907
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DNA Sequence : Show Sequence
>NC_006273.2:82065-84788 Human herpesvirus 5 strain Merlin, complete genome
TTCAGACGTTCTCTTCTTCGTCAGAGTCTTTCAAGTGTCGGTAGCCGTTTTTGCGATGTCGCAGTCGGTC
TAGTAGGTTGGGCTTCTGTCCCTTGTCCTGCGTGCCAGTCCGTCCGTCCAAAGAATCTGTACCGTTCTGC
TGCGCTCGCTGCTCTGCGTCCAGACGGGCCAGGGCCAGAAGCATCTGGTAAGCCTGCTCGTTGGTGTAAG
GCGGAGCCGCCGTGGATGCATCAGACGACGGTGGTCCCGGTCCTTTGCGACCAGAATTATAAACACTTTC
CTCGTAGGAAGGCGGAGCCTGTAACGACGTGTCTTTGGTGCTGCCCGACGTCACGGTGGTCCCGTCGGCG
GACACCAGATAGGGAAAGAGGTTCTGCAGCGGCTGCGTGCACAAACGCCGCTGTCGAGTATAGATCAAAT
AAGTGATAATGACTACAGCTATGGCCACGAGGATGATGGTGAACGCTCCGAAGGGGTTTTTGAGGAAGGT
GGCAACGCCTTCGACCACGGAGGCCACCGCGCCACCCACGGCCCCAATGGCTACGCCAACGGCCTTTCCC
GCGGCGCCCAGGCCGCTCATGAGGTCGTCCAGACCCTTGAGGTAGGGCGGTAGCGGGTCGACTACCTTGT
CCTCCACGTACTTTACCCGCTGCTTGTACGAGTTGAATTCGCGCATGATCTCTTCGAGGTCAAAAACGTT
GCTGGAACGCAGCTCTTTCTGCGAGTAAAGTTCCAGTACCCTGAAGTCGGTATTTTCCAGCGGGTCGATA
TCCAGGGCGATCATGCTGTCGACGGTGGAGATACTGCTGAGGTCAATCATGCGTTTGAAGAGGTAGTCCA
CGTACTCGTAGGCCGAGTTCCCGGCGATGAAGATCTTGAGGCTGGGAAGCTGACATTCCTCAGTGCGGTG
GTTGCCCAACAGGATTTCGTTGTCCTCGCCCAGTTGACCGTACTGCACGTACGAGCTGTTGGCGAAATTA
AAGATGACCACGGGTCGTGAGTAGCAGCGTCCTGGCGACTCCTTCACGTTCATATCACGCAGCACCTTGA
CGCTGGTTTGGTTGATGGTCACGCAGCTGGCCAGGCCCAAGACATCACCCATGAAACGCGCGGCAATCGG
TTTGTTGTAAATGGCCGAGAGAATGGCTGACGGGTTGATCTTGCTGAGTTCCTTGAAGACCTCTAGGGTG
CGCCGTTGATCCACACACCAGGCTTCTGCGATTTGCGCCAGCGCCCGGTTGATGTAACCGCGCAACGTGT
CATAGGTGAACTGCAGCTGGGCGTAGACCAGATTGTGCACCGATTCCATGTTGGATAAATGAGTTGCATT
GTTGCCATCTGTACTTCTTTTGGTTCTATTATGAGTAAGATTCAGACTGGAGCGGTTGGCCAAACGTTCG
AGTTCCACCAGAGATTTTTGCTTGATACCTTGCCAGAACACTACCAAACCACCAGTGGTTTCAAAGACGG
ACACGTTTCCATATTTTTCATATGTTTGATTGTATGAAGTATTGAAAATCTGCTGTAACTTATTTATAGC
CTCATCACGTACGCAGTCCAGCGCAGAGTCGGACATGTTCACCTCTTGCTTCTTAGATAAGAAAGTGGCG
GTCATTTTGGCAGAAGAAAAGTGATACGAGTCCTCGGCTTCGGAACGAATGGTGCGTTCCGAGGCTTCCC
AGAAAGTGAGTTGACAAGTGACATTCTTTTCGTCCTGTATATCCCAGGAGATCACCGAGTCCGCACGTTC
AAGAAAAGCCACCAACCTGTGGGTCTCTAACGCAGAATTCGGTCTTCCAAAGTCGGAGACGATAGTGTAG
TTCGGAAAAATGAAAAACTTGTCGGCGTTTTCTCCAAAGTAGCTGGCATTGCGATTGGTTCCGTTGTAGA
AAGGAGAAATGTCAACCACGTCACCCGTGGAAGTGGCGAAAAAATGATAAGGATATTTGGAGCGCGCAGT
AGTGATGGTCACCATACAATTCAGATTACAGGTCTCACGATAGAGCCAGGTGCTGCCGCGGCTGTGCCAT
TGATCCTTGACCGTCACGTAACGGGTACTGTGGGTGTTGGAATAATCGTCGGGCATTAATTGCATGGTTT
TGTTTTCATAGCTGTCCCTATGATAAGCCACGAAAACCGTGCCTGCTATAACGCGGCTGTAGGAACTGTA
GCACTGACTGTGGCTGTTGATATGATGAATCTCCCACATAGGAGGCGCCACGTATTCCGTGTTGCTGCCC
AGCAGATAAGTGGTGTGGATGTAAGCGTAGCTACGACGAAACGTCAAAACCTTCTGGTAGACTCGTACCT
TAAAGGTGTGCGCGACGATGTTGCGTTTGTAGACCACCATGATGCCCTCGTCCAGGTCTTCATTGATGGG
CTTCATCGAGGTGCAGACGATATTACGTTCAAAGCGAATAAGATCCGTACCCTGGGCCATAGAACACACG
CGATAGGGGTACTTGGTGGTATTGACCCCCACCACATCTCCGTACTTGAGGGTAGTGTTGTAGATGGTCT
CGTTAACACCATGGCTGACCGTTTGGGAAGAAGTTACGCGTTGAGAGACTGAACCGGATCGAGAGTGAGC
AGCAGACGTCGTATGAGAGGAATGGTGACTGTGAGTAGCAGAAGTTCCACGAGTAGAAGATGAGGAAACC
GCAGCACCCAGACAGACGATACACAAGTTAACGCAGACTACCAGGCACCAGATCCTGGATTCCA
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Protein Sequence : Show Sequence
>YP_081514.1 envelope glycoprotein B [Human betaherpesvirus 5]
MESRIWCLVVCVNLCIVCLGAAVSSSSTRGTSATHSHHSSHTTSAAHSRSGSVSQRVTSSQTVSHGVNET
IYNTTLKYGDVVGVNTTKYPYRVCSMAQGTDLIRFERNIVCTSMKPINEDLDEGIMVVYKRNIVAHTFKV
RVYQKVLTFRRSYAYIHTTYLLGSNTEYVAPPMWEIHHINSHSQCYSSYSRVIAGTVFVAYHRDSYENKT
MQLMPDDYSNTHSTRYVTVKDQWHSRGSTWLYRETCNLNCMVTITTARSKYPYHFFATSTGDVVDISPFY
NGTNRNASYFGENADKFFIFPNYTIVSDFGRPNSALETHRLVAFLERADSVISWDIQDEKNVTCQLTFWE
ASERTIRSEAEDSYHFSSAKMTATFLSKKQEVNMSDSALDCVRDEAINKLQQIFNTSYNQTYEKYGNVSV
FETTGGLVVFWQGIKQKSLVELERLANRSSLNLTHNRTKRSTDGNNATHLSNMESVHNLVYAQLQFTYDT
LRGYINRALAQIAEAWCVDQRRTLEVFKELSKINPSAILSAIYNKPIAARFMGDVLGLASCVTINQTSVK
VLRDMNVKESPGRCYSRPVVIFNFANSSYVQYGQLGEDNEILLGNHRTEECQLPSLKIFIAGNSAYEYVD
YLFKRMIDLSSISTVDSMIALDIDPLENTDFRVLELYSQKELRSSNVFDLEEIMREFNSYKQRVKYVEDK
VVDPLPPYLKGLDDLMSGLGAAGKAVGVAIGAVGGAVASVVEGVATFLKNPFGAFTIILVAIAVVIITYL
IYTRQRRLCTQPLQNLFPYLVSADGTTVTSGSTKDTSLQAPPSYEESVYNSGRKGPGPPSSDASTAAPPY
TNEQAYQMLLALARLDAEQRAQQNGTDSLDGRTGTQDKGQKPNLLDRLRHRKNGYRHLKDSDEEENV
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Molecule Role :
Protective antigen
- Related Vaccine(s):
Human cytomegalovirus DNA vaccine pcGB
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Human cytomegalovirus DNA vaccine VCL-CB01 encoding phosphoprotein 65 and gB
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2. IE2 |
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Gene Name :
IE2
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Sequence Strain (Species/Organism) :
Human cytomegalovirus
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NCBI Protein GI :
AAA45953
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Taxonomy ID :
10359
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Protein Name :
IE2
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Protein pI :
8.5
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Protein Weight :
2761.18
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Protein Length :
71
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Protein Sequence : Show Sequence
>AAA45953.1 IE2, partial [Human betaherpesvirus 5]
MMLRAWRLMVLLAAYCYY
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Molecule Role :
Protective antigen
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Molecule Role Annotation :
(Tang et al., 2017)
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3. pp65 |
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Gene Name :
pp65
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Sequence Strain (Species/Organism) :
Human cytomegalovirus
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NCBI Protein GI :
AAA45994
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Other Database IDs :
CDD:283448
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Taxonomy ID :
10359
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Protein Name :
pp65
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Protein pI :
7.17
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Protein Weight :
57622.66
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Protein Length :
603
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Protein Note :
Betaherpesvirus UL82/83 protein N terminus; pfam05784
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Protein Sequence : Show Sequence
>AAA45994.1 pp65 [Human betaherpesvirus 5]
MASVLGPISGHVLKAVFSRGDTPVLPHETRLLQTGIHVRVSQPSLILVSQYTPDSTPCHRGDNQLQVQHT
YFTGSEVENVSVNVHNPTGRSICPSQEPMSIYVYALPLKMLNIPSINVHHYPSAAERKHRHLPVADAVIH
ASGKQMWQARLTVSGLAWTRQQNQWKEPDVYYTSAFVFPTKDVALRHVVCAHELVCSMENTRATKMQVIG
DQYVKVYLESFCEDVPSGKLFMHVTLGSDVEEDLTMTRNPQPFMRPHERNGFTVLCPKNMIIKPGKISHI
MLDVAFTSHEHFGLLCPKSIPGLSISGNLLMNGQQIFLEVQAIRETVELRQYDPVAALFFFDIDLLLQRG
PQYSEHPTFTSQYRIQGKLEYRHTWDRHDEGAAQGDDDVWTSGSDSDEELVTTERKTPRVTGGGAMAGAS
TSAGRKRKSASSATACTAGVMTRGRLKAESTVAPEEDTDEDSDNEIHNPAVFTWPPWQAGILARNLVPMV
ATVQGQNLKYQEFFWDANDIYRIFAELEGVWQPAAQPKRRRHRQDALPGPCIASTPKKHRG
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Molecule Role :
Protective antigen
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Molecule Role Annotation :
(Tang et al., 2017)
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4. UL123 |
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Gene Name :
UL123
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Sequence Strain (Species/Organism) :
Human cytomegalovirus
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NCBI Protein GI :
P03169
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Other Database IDs :
CDD:284701
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Taxonomy ID :
10363
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Protein Name :
55 kDa immediate-early protein 1
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Protein pI :
4.44
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Protein Weight :
53577.61
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Protein Length :
582
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Protein Note :
IE1
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Protein Sequence : Show Sequence
>sp|P03169.1|VIE1_HCMVT RecName: Full=55 kDa immediate-early protein 1; Short=IE1
MESSAKRKMDPDNPDEGPSSKVPRPETPVTKATTFLQTMLRKEVNSQLSLGDPLFPELAEESLKTFERVT
EDCNENPEKDVLAELVKQIKVRVDMVRHRIKEHMLKKYTQTEEKFTGAFNMMGGCLQNALDILDKVHEPF
EEMKCIGLTMQSMYENYIVPEDKREMWMACIKELHDVSKGAANKLGGALQAKARAKKDELRRKMMYMCYR
NIEFFTKNSAFPKTTNGCSQAMAALQNLPQCSPDEIMAYAQKIFKILDEERDKVLTHIDHIFMDILTTCV
ETMCNEYKVTSDACMMTMYGGISLLSEFCRVLSCYVLEETSVMLAKRPLITKPEVISVMKRRIEEICMKV
FAQYILGADPLRVCSPSVDDLRAIAEESDEEEAIVAYTLATRGASSSDSLVSPPESPVPATIPLSSVIVA
ENSDQEESEQSDEEEEEGAQEEREDTVSVKSEPVSEIEEVAPEEEEDGAEEPTASGGKSTHPMVTRSKAD
Q
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Molecule Role :
Protective antigen
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Molecule Role Annotation :
(Tang et al., 2017)
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5. UL83 |
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Gene Name :
UL83
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Sequence Strain (Species/Organism) :
Human herpesvirus 5
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NCBI Protein GI :
270355835
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Other Database IDs :
CDD:283448
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Taxonomy ID :
10359
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Gene Strand (Orientation) :
?
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Protein Name :
tegument protein pp65
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Protein pI :
7.27
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Protein Weight :
58927.11
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Protein Length :
631
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Protein Note :
unpassaged strain in the original clinical sample;
acronym: HCMV; acronym: HHV-5
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Protein Sequence : Show Sequence
>ACZ79994.1 tegument protein pp65 [Human betaherpesvirus 5]
MESRGRRCPDMISVLGPISGHVLKAVFSRGDTPVLPHETRLLQTGIHVRVSQPSLILVSQYTPDSTPCHR
GDNQLQVQHTYFTGSEVENVSVNVHNPTGRSICPSQEPMSIYVYALPLKMLNIPSINVHHYPSAAERKHR
HLPVADAVIHASGKQMWQARLTVSGLAWTRQQNQWKEPDVYYTSAFVFPTKDVALRHVVCAHELVCSMEN
TRATKMQVIGDQYVKVYLESFCEDVPSGKLFMHVTLGSDVEEDLTMTRNPQPFMRPHERNGFTVLCPKNM
IIKPGKISHIMLDVAFTSHEHFGLLCPKSIPGLSISGNLLMNGQQIFLEVQAIRETVELRQYDPVAALFF
FDIDLLLQRGPQYSEHPTFTSQYRIQGKLEYRHTWDRHDEGAAQGDDDVWTSGSDSDEELVTTERKTPRV
TGGGAMAGASTSAGRKRKSASSATACTAGVMTRGRLKAESTVAPEEDTDEDSDNEIHNPAVFTWPPWQAG
ILARNLVPMVATVQGQNLKYQEFFWDANDIYRIFAELEGVWQPAAQPKRRRHRQEALPGPCIASTPKKHR
G
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Molecule Role :
Protective antigen
- Related Vaccine(s):
Human cytomegalovirus DNA vaccine VCL-CB01 encoding phosphoprotein 65 and gB
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| III. Vaccine Information |
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1. Human cytomegalovirus DNA vaccine pcGB |
| a. Vaccine Ontology ID: |
| VO_0004599 |
| b. Type: |
| DNA vaccine |
| c. Status: |
| Research |
| d. Host Species as Laboratory Animal Model: |
| Mouse |
| e. Gene Engineering of
Glycoprotein B |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
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| f. Vector: |
| pcDNA3 (Hwang et al., 1999) |
| g. Immunization Route |
| Intramuscular injection (i.m.) |
| h.
Mouse Response |
- Vaccine Immune Response Type:
VO_0003057
- Efficacy:
Neutralizing antibody was developed, and the percent reduction of input infectivity in 1:100 diluted sera was 74.5% in three-times immunized groups. The induction of neutralizing antibody with HCMV gB in this study might be useful to attenuate or prevent HCMV infection (Hwang et al., 1999).
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2. Human cytomegalovirus DNA vaccine VCL-CB01 encoding phosphoprotein 65 and gB |
| a. Vaccine Ontology ID: |
| VO_0004341 |
| b. Type: |
| DNA vaccine |
| c. Status: |
| Clinical trial |
| d. Host Species as Laboratory Animal Model: |
| Human |
| e. Gene Engineering of
Glycoprotein B |
- Type:
DNA vaccine construction
- Description:
This DNA vaccine expressed the major hCMV surface glycoprotein B (Schleiss, 2009).
- Detailed Gene Information: Click here.
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| f. Gene Engineering of
UL83 |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
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| g. Immunization Route |
| Intramuscular injection (i.m.) |
| h.
Human Response |
- Vaccine Immune Response Type:
VO_0000286
- Immune Response:
VCL-CB01 increased T-cell responses compared with placebo (Schleiss, 2009).
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| i.
Mouse Response |
- Vaccine Immune Response Type:
VO_0003057
- Efficacy:
Preclinical investigation of the hCMV gB/pp65 DNA vaccine was initially conducted in mice. When formulated in PBS, lone gB and pp65 plasmids were highly immunogenic, but vaccination with the bivalent vaccine reduced immune responses to each antigen by 2.8- and 4-fold of the lone values, respectively. However, formulating the bivalent vaccine with 02A significantly (p = 0.048) increased the antigen-specific immune responses relative to those induced with the bivalent vaccine in PBS, as well as completely abrogating a decrease in pp65-specific T-cell responses (Schleiss, 2009).
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| IV. References |
1. Hwang et al., 1999: Hwang ES, Kwon KB, Park JW, Kim DJ, Park CG, Cha CY. Induction of neutralizing antibody against human cytomegalovirus (HCMV) with DNA-mediated immunization of HCMV glycoprotein B in mice. Microbiology and immunology. 1999; 43(3); 307-310. [PubMed: 10338203].
2. Mayo Clinic - CMV: Cytomegalovirus (CMV) infection [http://www.mayoclinic.com/health/cmv/DS00938]
3. Tang et al., 2017: Tang A, Freed DC, Li F, Meschino S, Prokop M, Bett A, Casimiro D, Wang D, Fu TM. Functionally inactivated dominant viral antigens of human cytomegalovirus delivered in replication incompetent adenovirus type 6 vectors as vaccine candidates. Human vaccines & immunotherapeutics. 2017; 13(12); 2763-2771. [PubMed: 28494195].
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