VIOLIN Logo
VO Banner
Search: for Help
About
Introduction
Statistics
VIOLIN News
Your VIOLIN
Register or Login
Submission
Tutorial
Vaccine & Components
Vaxquery
Vaxgen
VBLAST
Protegen
VirmugenDB
DNAVaxDB
CanVaxKB
Vaxjo
Vaxvec
Vevax
Huvax
Vaccine Mechanisms
Vaximmutordb
Vaxism
Vaxar
Vaccine Literature
VO-SciMiner
Litesearch
Vaxmesh
Vaxlert
Vaccine Design
Vaxign
Community Efforts
Vaccine Ontology
ICoVax 2012
ICoVax 2013
Advisory Committee
Vaccine Society
Vaxperts
VaxPub
VaxCom
VaxLaw
VaxMedia
VaxMeet
VaxFund
VaxCareer
Data Exchange
V-Utilities
VIOLINML
Help & Documents
Publications
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

Experimental autoimmune encephalomyelitis

Table of Contents
  1. General Information
    1. Disease
    2. Introduction
  2. Vaccine Related Pathogen Genes
    1. Mog (Other)
    2. MBP from guinea pig (Protective antigen)
    3. Mog (Protective antigen)
  3. Vaccine Information
    1. EAE DNA vaccine pZZ/MBP68–85 encoding MBP
  4. References
I. General Information
1. Disease:
Experimental autoimmune encephalomyelitis (EAE)
2. Introduction
The experimental autoimmune encephalomyelitis, sometimes experimental allergic encephalomyelitis (EAE) is an animal model of brain inflammation. It is an inflammatory demyelinating disease of the central nervous system (CNS). It is mostly used with rodents and is widely studied as an animal model of the human CNS demyelinating diseases, including multiple sclerosis and acute disseminated encephalomyelitis (ADEM). EAE is also the prototype for T-cell-mediated autoimmune disease in general. EAE was motivated by observations during the convalescence from viral diseases by Thomas M. Rivers, D. H. Sprunt and G. P. Berry in 1933. Their findings upon a transfer of inflamed patient tissue to primates was published in the Journal of Experimental Medicine article (Vol. 58, No. 1, pp. 39–56). An acute monophasic illness, it has been suggested that EAE is far more similar to ADEM than MS. EAE can be induced in a number of species, including mice, rats, guinea pigs, rabbits and primates. The most commonly used antigens in rodents are spinal cord homogenate (SCH), purified myelin, myelin protein such as MBP, PLP and MOG, or peptides of these proteins, all resulting in distinct models with different disease characteristics regarding both immunology and pathology. It may also be induced by the passive transfer of T cells specifically reactive to these myelin antigens. Depending on the antigen used and the genetic make-up of the animal, rodents can display a monophasic bout of EAE, a relapsing-remitting form, or chronic EAE . The typical susceptible rodent will debut with clinical symptoms around two weeks after immunization and present with a relapsing-remitting disease. The archetypical first clinical symptom is weakness of tail tonus that progresses to paralysis of the tail, followed by a progression up the body to affect the hind limbs and finally the forelimbs. However, similar to MS, the disease symptoms reflect the anatomical location of the inflammatory lesions, and may also include emotional lability, sensory loss, optic neuritis, difficulties with coordination and balance (ataxia), and muscle weakness and spasms. Recovery from symptoms can be complete or partial and the time varies with symptoms and disease severity. Depending on the relapse-remission intervals, rats can have up to 3 bouts of disease within an experimental period (Wiki: Experimental autoimmune encephalomyelitis).
1. MBP from guinea pig
  • Gene Name : MBP from guinea pig
  • Sequence Strain (Species/Organism) : Cavia porcellus
  • NCBI Protein GI : 3309629
  • Other Database IDs : CDD:145029
  • Taxonomy ID : 10141
  • Gene Strand (Orientation) : ?
  • Protein Name : myelin basic protein
  • Protein Length : 150
  • Protein Note : MBP
  • Protein Sequence : Show Sequence
    >gi|3309629|gb|AAC26130.1| myelin basic protein [Cavia porcellus]
    SQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGRFFGSDRAAPKRGSGKDSHHAARTTHYGSLPQKS
    QRSQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQKPGFGYGGRADYKSKGFKGAHDAQGT
    LSKIFKLGGR
  • Molecule Role : Protective antigen
  • Related Vaccine(s): EAE DNA vaccine pZZ/MBP68–85 encoding MBP
2. Mog
  • Gene Name : Mog
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 17441
  • NCBI Protein GI : 113199771
  • Genbank Accession : AC116130
  • Protein Accession : NP_034944
  • Taxonomy ID : 10090
  • Chromosome No : 17
  • Gene Starting Position : 37010739
  • Gene Ending Position : 37023397
  • Gene Strand (Orientation) : -
  • Protein Name : myelin oligodendrocyte glycoprotein
  • Protein pI : 8.06
  • Protein Weight : 26389.6
  • Protein Length : 247
  • Protein Note : Also known as B230317G11Rik
  • DNA Sequence : Show Sequence
    >gi|372099093:37010739-37023397 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J
    GAAGTATGCACAGAGAAACTTGTATTATTAATTAGCACCTAGCTTGTTTGTGTCTGGTAGACCACCAAGT
    AGTAATTTTTGGGGGAAAAAAACCACATTGGTTCTCAGAGAAATAAGGTAGGAGAAAATGAGAGGCAGGG
    GTATGAAGGGCACGAAGGTGTGGAACATAGGGGAGCAGACCCAGTTCTCAGCCCTTGCTTCTCAAGGTCC
    TCCCACCAGGCTGCCAAAGCAAGATGGTATTTCTCTGGTCGGTATTCTTTTGAATTTTAGCTCCAGGTTT
    TGGTTCAGGTGCTCATATTCATACCCTGGCTCACTTAGCTTTCCCCGATATTGTGGGCTCTTCTGTTCCT
    GACCCCCCCCCCCCCACATTGATATTCTTCCCAGTCAAGGCTGTGGTCTTGGAAGAAATATCCACCATTT
    GCAGAGTTGATGTTCTATAGTTCACAATGATGAAGTGCTGCGTATCCTGGTTGGCAGAATCACTCCTGTA
    TTACTCTGGTGCATAGGTGTCTCCTGATAGACTGCCTGGCCTTCACCACAGGGTGTTTTCTATAGTCAGA
    CTAACATGGTAAAAAAGATGTGGAAGTCAAAAATTAGAGGAAGGAGCTGAGGAAAGACAGTTGAGGGAGG
    GAAATCAAGTTCTACTCGGTATCCAGAATGTGTCTGTAGATGTAGATTGGAATCTGTCCACAGCAAAGAG
    GCCAATGGGAAATCCCCAAGGACCTGCTGGGCTCTCCTTCCGCTCCAGGAAGACACAACCATCACTCAAA
    AGGGGTTTCCTGGAAAGACAGACAAGTGACTTTAAAAACCTCTGCCAGTAGGTTCTTTTTATGAAGTCCT
    TGAACCTAGGAGATAGAAAGTTCCTAGGGGTCATGTGATCTCTCCCCCAACCGCCTGGTTGGTCTTGGAG
    GGAGGTGAGGTTCGGGATAACTATTATGGAGAATGTTAAAGGGGGCTGCAGAAGTGGAATTCTGGCTCAG
    GGGCACTAAAGGCTATTAAGTCAACAGGTAAGAGTGGCTCAGCAGCCTCTCTGGCACTGAAAATGTGTCT
    CGTTAATTCCCTCCCCTTTTCCCCTCACAAGAGGAGTCATTCCAGCCTTCCCTGGGATAATGGTCCCTCA
    GTAACCATGTTCCCTTTGCTGGGTTTTTGTTCGATTTAGAAGGAGGGAAATGGTTGGGTACAATGGTATG
    TGCTTAAGACGGTAACACTTGGAGGCAAGAAGAGGGTTGCCATAGTTTCTAGACCAGTTTGGTTTACATA
    GTAAGTTCTAAGGCTGCATAATGAGATGTCTCAAGATTGGAGAGGGGTGTTAGGGAGAACTTACTTAGCT
    CTTCAAGAAACTGTCCTGTAAACAAAAAGAAATGAGTCAATGGGTGGTCAGTGGGAGTCCCCCCTCACCT
    CCCCAGACAACCCTCCCTCCCCATTAAGAGGCCTATACATCTCATTAAGTGGTTCTCCGTTTTCCTATGG
    ATTCTGATGGTGAGAGTAAAGATTCCCATCCCTGGAATCTAGAACCTTGGTTCCACTCTTTTCCCATACT
    GGTCTGCCTGAGTTCCCTGATGCATAGCAGTCCTGTTTTGCTGCCCAGACACCGAACCTGCCAGTCTTCG
    GTGCAGCCAGTTGTAGCAGATGATCAAGGCAACCAGGGGTCCAAGAACAGGCACAATAACAAACAGTGTT
    ATCTTCCAGCAGGGCACCCTCAGGAAGTGAGGATCTGAGGTAAAGCAGAGCAGAGTGTCTCCCTCTGTGC
    CACGCCCATTCTTAACCTGAAGCCCCGAAATGACAAAGGACTCTGGGGACAGGCTGTAGAGTCCGTGAAC
    ATGGCTGGGGTGCACTGTCCCTTTATTTTCATTAACTTCTGATTGAATTTAGCTTTTTAACTGATTTATC
    ATAGAAAACAGGTTGTGTGACTTTTCATGCATTGTTTATGTTCAGCCATGCATTGACCCTCCCTCACCCC
    AATCTCTAGCATTTTTATTTGTTTGAAGAGAGGTTCTTGCCCTGTATCCCTGGCTGGCCTTGAATTTGAG
    AAATCCTGTCTCAGCCTTACAGGTGTTGTGGTTACAAGTCATCAAACCCTGCTTTCCCTAAGGTTTTCTT
    TTTCTGTGTGGCAGAGGTTTATTTGGAGGTGGGGGTGGAGACAAAGGGACAAAGAGAAAAGGAAAAAACA
    GGGCACGGTTTTCTTATACTAACAGACTAGGGATGCAGTCTCAAAAAGAAAGAAACCAGCAAAAACAAAA
    AGTCAAAAACTGGTATTAGGACTGACTGTATGATTTTAAGATACAAACATGGTTAAGAACTTAGACATTC
    AAATCTTTTTTTTTTTTTTTTTTTTTTGGAGGCAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGAACTC
    ACTATGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTTCCAAGTGCTGGGATTAAA
    GGTGTGTGCCACCACTGCCCGGCTAGACATTCAAACCTTATAAGTAAAATCCTGGGGGTTGGGAAGGTAG
    CTCAGTTTGTAGAGCTCTGAGTTCCACCCCCAGCACAGCATGCACTAGGCTTGGTGGCTCACACTTATAA
    TCCCAGAATATAGGAGGTGTAGGCAGGGGGATGATCAAAATTCAAGGTAATCTTTGGCTACATAGTGAGT
    TTGAGTCCTTCCTGAAATACATGAGACAGACATTTCAAAACAAAATGGAAATGGGAACGGTGAGATGGTT
    TAGCTGATAAAGGCACTTGCTGCTAAACCTGAAGACCTGAGTTTGACCCCAGACCCACATGATAGGAGAG
    AACCAACTCCTTTATATTGTCCTCTGACCTCTGTGTGAGCACTTGCCCACACTCACCCACTCACACCCTC
    CACACACACAATAAAATACATTCAAAGACTACATGTGTGATGTCCATATATTGGCCCAATGTCAAGTGCT
    AGGGTGTAGCCAAGTACCAGGAGAGCTGAACAGGGTTGCATCTGGCTTCAGAAGGAAAGGCTGTTTATGT
    TTAGGAAGATTGATTAGAGCAGGATTCTGCTATGCTCTGCTTAATGTTATTTTTTTAGTTGTGTGGAATC
    TATTCAACAAACGTTTGGGTATTCTTCTGTTGCTGGTGTTGTCCTTAGGAACTCTGCAATCTGAAGTGAA
    GAAAATGCGAAGATGATTCTTGAGGACTTAATATTCTAGTTGGGGCATGCATGGTTGAGTATACATTTAC
    CAGAATCTTGGGAGTTGAAGGTAGAAGGAGAAGAAGTCCAAGGCCAACCTTCATGACATAGTGAGTTTGA
    AGGCCAGCATAGCCATATGAAACCCTGACTCAAAAAGTTACTAGTGAGGTTTCATTTTCTCCTCAAAGAA
    GACTTCCCTGTTGAATCATGTGCTTGGACTACATTCCTGAAAACTAACTCTGCCATATAAGTATAGCAGA
    TGACTTTTTTTAAATGTAATTTTCTGGATATGGTGGCACATGTCTTTAATCCCAACACTGGGGAAGGAGA
    GTGTGTGGAGCTCTGTAAGTTCAAAGCCAACCTGTTGTTCATAGTGAATTCCAGGTTAGCCAGGGCTACA
    CAGTGAGACCATAACTTAAAATTTTTTTTCTTACATATGTATTCCTTGTGCATATAGCTGTTTGCTGTGG
    GGTCAGGAAATGGAACTTTAGGTCCTCTAGGGGAGCAGCAGGTGCTCTTAACGATTTTTCCCACCAGCAA
    GCAATTTTATGTGCAAAGGATCTCTTTAACCACTTTTTATAATTCAGAAGATGAATATATATACATATAT
    ATACACATAAGTACATATATGCATGTATACATATAAAGTTTACAATTGGTAAAATTCAGTGTAGGGCCTG
    ACACTGGAATATGGTACATTAATGATTGCTACATTCCTATAGATCTTGAATGTGAAGAAAAAAGGTGGAG
    GGGCCTTACAAAAAGCCACTGGTTCTAGTCCCCCAGGCCACTAGTGAAGTCCCACGGTGACTTGGGAGGG
    TTGGGATATGCCTGAACTTACCAAAAGTCCGATGGAGATTCTCTGAAAAGGACAAATAAGAATAGACTTA
    GTAAGGAACTGAAAGGGAGTCCCTTCCTTGGATCTATCTAAATCCCTCAAGGTTGGTTCTAGCCCTGATG
    CAAAGTAAACCGTGCTATAAGAGCTCGGGTGAAAAAAAAATCTAATTTTTAGCTGTCTATAGTCTTAAAT
    ATAAAGTTCCCAATTGAAGGGAGCATGGAGCATTGGGGGAAAGGCAGAAATGGTCTGAAGAGAGGGATAT
    TTTGGTTAAGTTATAAAGACCAGAAGAGGAATTGGGGAGGGAGGGTGTCTGGAACTCACCTACTTCTGCA
    CGAAGTTTTCCTAAAATAGAAAAAAGCAGGTATTTTAGTTCCTGATCTAACAGTATGAGGACCCTTCCAT
    ACCACCCAGCACCTTGGACCTTTGTGTGGAGAATTCTTTGATGGCCCGGGAGCATCTTAGAATAGGAAGA
    GCTCTAGATTTGAAACCAAGAGTCCAGTCTCTTTCTGTGAATATTGTCTGATTAAATAATGAAATAAAGA
    GCATGAGAGCCTGGAACTGATAGTGATTGGTTGCGTTGTCTGTCATCACAGCAAGGACAAGGCTGATTAT
    GTCGATCTAGCTGGCTTCTTTTCTTACAACTCCGTATGTGTCCCTCCCAAAACTGTTTGCTTGGCTGGAG
    AAAATGACCTTCCAGAAGAACAGGGCCCACTGTTCTTTGGTTACAGGTATAACAAATTGCTGCAGTTCAC
    AACTGGAACCTCCAAACCTTTTTGCATTTAAGAATGCAAAAGTCTGGGGCTGGTGAGATGGCTCAGCAGG
    TAAGAGCACTGACTGCTCTTCCGAAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCA
    TCTATAACAAGACTGGATACCCTCTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATATCGTAAA
    TAAATAAAGCTTAAAAAAAAAAAAAGCAAAAGTCTCAGGAACCTGCAATGTTCCTAACATACCACATGGT
    TGGCTTGTTCTTCTTCTTTTATTTTTAATTCTATCTGTGTCGCAGTGAGTGTGTGCGTGTGTCTGTGCGT
    ACACATGAGTATGTGTGCCCCTGGAGTCCAGAAAAGGTTGTCAGATCTCCTAATGTTATGGGTGGTTATG
    AGTTGCCTGATAGGTGCTAGGGCTAGAACTTGAGTCCTCTGTAGGAGCAGTACAGGCTTTTGGCCTCTGA
    GCTGTCTTTCCAGCACTATATCATAAAGCTTTATCACTTCTTCCATTATTTCTTGCTCAGTTCACATGGT
    CAAGAAGATTGTCATTTTGTAAATATAAACCCATATCCCATCTGGAGGAGAAACTACACATTTAGTGAGC
    AGCTACTTTATGATTGCATGTATGCTACACACTTCTCCCTGTAAGATTCCCACAGTTTAAGAGGGAAATT
    CTATCCCTACTTGGAGACTTGGAGAACCTAAGAATTTTTCTCATAGTTGGTGTCTACAGTAGAGTTGGAT
    CCTAGGCTGCCTAGCTCCGTGTTCCTGCTCTAGTAGCCATGATGTTGGAGAGCCTTTTCTTTGCCATGGA
    GTAGGTAACCAGAAATTCTGGGTGAAAAAACAAAAAACAAAAAACAAAAAACAAAAAACAAAAAACAAAA
    AACAAAAACAAAAACAAAAAACAAACAAAAACTAACCCTCACTGTGGGGCTGGGGATGTAGCACAGTGTG
    GAGAACCTGCCTGTTGTGTATAGGGCAGGGCCATTGTTGCTCTTTTGTCCTTTCCATGACTCAAACCCTC
    CTTGCTGCTATATTCTCTTATCAGCAGAAGGAAGTGGAAATGCAAGAACTCTTGCCTTGGTTTGGGAAGG
    ACACAGCACAAGGAGACTCTGACTGCAGGCTCTGGGCACAAAACAACTTCCTCTACAGCTGAATTTATTA
    TGTTCATAGTTGAATGGAAAAGACAGGGGAACCAGTTGTTTAATACATTCCATGTGGCAAAGACTTTCAT
    TTCAGAATCTTTATTAAAACATGAAGTAGTGGGGCTGGAGACATGGCTCACGGTTAAGTGTGTTTGCAGC
    TCTTGCATAGGACCCAGGGTATAGTTTTTAGTACCCACAGAGAGCAATTTACAGCCACCTATAGCTCCAG
    GGTCTCAGACACTCTTCTGGTCTCTTGCAGTACCTATAAATATGTGTGCACATATAGAAAAGCACAGACA
    AAAAATATATGTGTGTGTGTGTGTGTGTGTGTCTGCATATAGGTATATGCACATGACTGTAGGTGCCTGA
    GGAAGCTAGAGATGTTGGATCCCGTGGAGCTAGATGGTTGTTATATCATGGTTACAGATGGTTGTAAGCT
    GTCTAATATGGGTTCTGGGTCCTCTAAAAAAGGAGCAATTGCTCTTAACCATGGAACTATCTCTCCAGTC
    ATATATATATGCCTAGTATTTTAGACCTGACTGACTTTGAAGAGACTACTCACCCAGTTTCATGAAGCTG
    CTAAGGACTATGGAGGGCAGAGCTCTACTGCCTGTAACATGTCATCTGAGCAAGTCACCAATCCACCCCT
    AGACTCAATTTCTTCCTCGGCAATGAGGCTGGCACTATTACTCCACAGGTAGATGCAAGGTAAATTGTTC
    AGTAGAACCTGTTATGTAGTTCTGAACTGAGAAGACTTAAGGATTAACTGTCCCTAAGAACCAGGAAACG
    ATCCTGACAGACCCACCCGCTGCCCATACCTCTCAGTCTGTGCTGCAGGAAGAGGAATACAAGGCCTACA
    GAGACCTGCAGGAGGATCGTAGGCACAAGTGCGATGAGAGTCAGCACACCGGGGTTGACCCAATAGAAGG
    GATCTGAAAGGCAGAAGGAACACGTCACCCAGACTGCTTCCTAACACCCTGTAAAGAGCTTGTGCTGCAG
    TAGGTTGGGTTGAATAAGGGAACCAACATCAAGATTTTAAAAATACTGACACACCCAGGCATGGTCTACA
    TACATGACTCCCAGCATTCTGGAGTCTAAAGCAGGAGGATTGCTGCAACTCACTGGACAGCCTGCAAGAC
    ATGTCTCAATAAAGAAGGAGGGTGACATAAAGTGAAAATGTTGGTAAAGTAAACTTGTTTTTAAAAGAGA
    TGATGTGGCCATTGTTTTTTGTTTGTTAGTTAGTTTGTTTGTTTTGTTTTGTTTTAAACAGGGCAAGTCT
    GTCCTGAACTCTTTGTCCTCTAGTTTCTACCTTCCAAATGCTGGGAATTCAGGCCTGAGCTACAACTCCC
    CAAATTTTATTCAGTGTTTTCAAAGGAACTCCAACCAATCTTTGTGTCTGTGTCTATGTCTGTGCCTGTG
    CCTGTGCCTGTGCCTGTGTCTGTCTGTCTGTCTGTCTGTCTCTCTCTCTCAAGCTAATTAGAGGAATTTG
    GAATGTGGACACCAACTCAAAGGAAAGCACTCTCCCCTGAGGCACAGTTTTCATTGTGTGTTCTGCCCCA
    CTCCCACCACCTTTCTTATTTGTAATTTGTTGGGTCTCAATGAGTGTACCGGAGTTGGCAGATGAGTTGG
    GAATTTGCTAATCAAGAAGAGAGAAAAAAAAAATCTGTATGCTAAAGTTCTGACCTTCCACTTTTTGTGT
    GAGTAAAAATTCTCCAGGAAAAAAAAAAAAAAAACCCAGCTGTCTGAGGAACATTCTGTGGCACACAGCT
    ATTTTCATGTCCCTGCAAGCTGTCTCTAGAAATAGAGCTTTGTTACTGGGCATACAATGCATCTTCTGGT
    CTCAGCCTGACAACAGTCACCACCACCTCAGATACAGAAATGATGGATTGTCTGATGTTCACACTGCAGG
    TGCTGAGGAGGAAGGGAAAAGATCAGAGGCCCAGCACTCACAACACTATTGTTCTTCAATTGCATCGCAA
    TTAACTTTCTATACGCTCCTTCTGTCTATTTTCCTATCCATGGTTTAACGACTCAGAGGCAGGACAAATA
    ATGAATAACTCCAAAACCAGCCACCACACAAAGCATACTGAAAATAAAGTTATAAAGACAAAGAGCAGTG
    TCATCGAAACTACAGGTCTCCTCCACTACTGTGTATGTGCTTAATTTTTACTTAGGTGTATATTTGTGTG
    GGGGGTATTGGCCTTTGGAAGCCAGAGAAGGCATCGGATCCCCCAGAACTGGAGTTATGTGGGTACTGGA
    ACCAAACTCTGGACGCCTGCAAGAGCAACAAAAGCTCTTAAGCACTGAGCCATCTCTGCAACCTCTTGGT
    TTTATTTTGTTTCGTTTTAGACGGTCTTTTATATTCTAGGTTAGTGTCTGACTGACTTCTCACCCTCTTG
    GCTTTAACTTCTAAGTGCTATGATTACGGGCTTCAGGTTCTAAGATCAGTTTATGCAGTGCTGGGGACTG
    AACCCGTGTTTACTTCCATTCTAGGTAAGAACTCTACCAAATGAATTATCCCTGCCATATATTACATACA
    CACACACACACATTATATTCCTGTCATACATATAATCCACACACATATATGCATATATATTGAATACACA
    CACACACACACACACACACACACACACACACACACACACACATATATCCACACTCAGACACAGGGATATG
    TTTTTGTTCCCGATACTCTATAGCTAGCTTATGTAACTCGACAAGCTCTCATGGGAAGCTATCTGGTATG
    TCTCTAAAGCAATCATTTTAAGCTGAGCATAGTGACTCATGCTTTTAGTGCCAGCACTTCAGAGGCTAAG
    GATGGAGGATTGCCCAAGTTATATATATATAAAAAGTTAGTTGTGCCAGGCGGTGGTGGCGCACACCTTT
    AATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAAAGTGAG
    TTCCAGGACAGCCAGGGCTATACAGAGAAACCCTGTCTCGAAAAAACGAAAAACCAAAACCAAACCAAAA
    CAAAACAAAACAAAAAGCTAGTTGTGGCAACACATGCATTTAATCCCAGCTCCCAGCTCTTTAGGAGGCA
    GAGACAGGAAGACCATTGTGAGTCTGAGGCTAACCTGGTCTATATAATGAGTTCCAGGCCAGCCAGGTCT
    ACATGTTAAAAAGCCATCACTTAAAAAGCCAGATAGTCAAAAAGAAGAGCTACCTGGCCGGGTGTGGTGG
    CGCACGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGAGGATTTCTGAGTTTGAGGCCAGCCTGGTC
    TACAGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAAAACCAAAAAAAAAAAA
    ATAAAAAAATTAAAAAAAAAACAAAAACAAAAACAAAATAAAATAAAAACCGAAGTGCAAGAGCATTTGT
    CTAGCTTAGATATCATCTTTTTATCTAATGTCACCTTCTTAGCCATGAAAAGGGACTTTGATTCCAGTTT
    CTTTCCCAATTCAAATAACAAAATAACCAACAGCCATAACATAGGCATCCCTGTTTACTGACGTGTCTGT
    TCCTTTGGATTTGATCTCAGAAGTGACAAGTTTGGGTAGAAAAACCTTATCTCAGCCGAAGGATGAAGGG
    AGGTCAACTATCCTACAGGTCATTTGACCTTGGAGGACTCACCTTCCACTTTCAACTCCATTGCTGCCTC
    TTCTTGGTAAGAGTGGTCTCTGAAGAAGCAGGTGTAGCCTCCTTCATCTGAGAATCTCACGTTCTGAATC
    CTAAGGGTAACCTTTCCCTCACTGATAGTCTCTTTCAGAAGCTCTGTGCGTCCCCGGTATTCAGGTGCTT
    GCTCTGCATCTTGGTCCTTGCCATTTCGGTAGAGGTGAACCACTCTTGAGAAGGGAGAACGGTACCAACC
    CACCTCCATGCCCGTGGCATTTTTCCCAGGAGAGATGCGGCACGGCAGCTCTGCTTCATCCCCAACTAAA
    GCCCGGATGGGATACCCTGGTCCTATCACTCTGAATTGTCCTGTCCAAGACACAAGAGTGGGAAAAAATA
    ACAACAACAAGTATCAGATGTGACAAATTCATCCATCATAAGAAGGCTGCTTCAAAAAGGGTGAGAGGGC
    CGGGCGGTGGTGGCGCACAACTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCGGACTTCTGAGTTCGA
    GGCCAGCCTGGTCTACAAAATGAGTTCCAGGACAGCCAGGGCTATTCAGAGAAACCCTTTCCCCCCTCCC
    CCCCCCAAAAAGGGTGAGGAAGGAAACATTTTTCATTTTTATTTTATTTTTGAGACAGGATCTCACTGTG
    TAGCCCTGGCTGGGCTAGAACTCACAGAGATCTGCCTGCCTTTGCCTCCCAAGTGCTAGGATCAAAGCTG
    TATACCACCACACTCAGTGGTTTGTTTTATTTTAAAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
    GTATCAGTACACACATGAGTGCAGGTGACTTCAGAGGCCAGAGGCATTTGTCACCTCCCCCCCCCCCCCC
    ATCTCCAGTCCTTTGTAAGTGAAGTATATACTCTTTACCACGGAGTCATCTCTTGAGCCCCTATTTATTA
    ATTTTGAGAAAGGATCTTGCTCTGTAGTGCAGATTGGTCTTGCACTCATAGCAATCCTCCTGTCTCGCCT
    TTTTAAGTGCTGGAATTATGAGCATGTGCCACTGTGCTTAGCTTAGGACACTCAATTTTTTTGGTAAAGG
    TTTTGCCTCTGTATCAGAGTGGGTGACTCTATGTCACCTTTGTGCAGCTACTCTTGGAACCCATAAGAGG
    CTGTGAGATCCCTTGGAGTTGGGGTTACAGGTAGCTGTGAGCTACCCCATAAATGATGGTACTGAGACCC
    CTACCTTGGATCTTCTGCAAGAGCAGCGAGCATACTTAACTGCTGAATCATCTCTTCAATACCTCTCCTC
    ATTATTTGATTTTTTTTTTTTTTAAGACATGCTTATAGTTCAAGCAGGCCTTAATCTTCCATGTAGCTGA
    GACTGACTTGAATTTCTGGTCCTTCTGCTTCCATCCCACAAATGCTACAATTATAAGTAAGGGTTCACCA
    CCAGGCCAGGTTTGGGAATATCACATGGAATTCATGAACCCTCAGTAGGGCTTTTTTTTTTTTTTTTTAC
    TTTAAGCTAATATGAAGTAAGCAGTCTCTAGGAAAAGATATGTTCTGAGCCGGGCGTGGTGGAGCACGCC
    TTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCAAGGCCAGCCTGGTCTACAAAGT
    GAGTCCCAGGACAGCCAGGGCTATAAAGAAAAATCCTGTCTCGAAAAACCAAAAAAAAAAAAAAAAAAAA
    AAGATATGTTCTGAATGTCTAGGACAGAATGCTCCACCATGAAATCATCTGACAATGGCTTCCACTTTTC
    TACCAGCTATGTTCATCTCTGACTCCTCAGCTCAGATGCTTGCCTCCTTGTCATGTGGCCCACTACTTCT
    AAGTGCTTCCCAGCGCTCTAGGTTGTTGGGAGTCACCCCATGGATGTGGGTGCCGGTAATCAAACTCCAG
    TTTCCCATAAGAGAAGTAATACTCTTTACCACAAAGCCATCTCTCTAACCCCTATGTATTAATTCTCTTC
    TTTTCATTTGTATCTTCAGCCTTATGCTAGCTCTGGAAACTTCTCTGGACATCTCCCCAGTGAGAATCAG
    CTACTAGCAAACTGGGCTCTTCCTTATACCAGGATGCCCACAGAGGATGCCTGGACTAGGGCGGTTTCTT
    TATTTCTTCTTTTAGTTGGACAACAGGCTCCTGTATCTCTGCCTTATTCTGCAGAGCAAGTTCTCAAACA
    TAACTCGATGCTATAACTCAACTTGTTTTTGTTTTTGTTTTTAAATATATTATTTTATTTGTGTTGTTTC
    ATTTTAGCATTCAGGCTTAGAGACAAGCACATTTGCCCACTGAGCCACCTCACTGACCCTCAACAAACAT
    TTATTGAGATCTGCTACAAACAGGCATTGCCTTAATGTGCAAAGTACAAAAATACTCTCACTTAACTCTG
    ACGGTAACTCCAAGAAGTTAGTGTCCTCTTTTGAACGATGAAAAAAGGAAACGAAACTAGGGCTACAGAT
    AGAGCTTAGTTGTGAATTGTGAACTTAGCTTGCACCAGGCCCTGAGTTTAATTCCCAGTACCATATGAAT
    AGGGAAAGGTTTGGGTTTACCCGCCCCCCCCCCCCAAGAAGTCAGGAAGGCCTTTAAGGAGAGAGACTAT
    GCCAACAGACCCAAGCATAAACAGGAAGTTTGTATAGCCATTTGAGGATGATTAAAAGAGGTTAGCCAAG
    GACACTCCCTTTCTGCTCCTAGGTCTCTGCAGGGGTGGAATGAGCATGCTTACCTGCATAGCTGCATGAC
    AACTGGAGGAGAAGGAGGAGGAGAAGGGAGAGGAAGCAGCTGGGCCAAGAGAAGCTCCACAAACAGGCCA
    TCTTTATGGTTACCGGGGACAGAGTCCCTAACAGCAGGGTCAGGCCAAGCTGAGGTCCTCCGGCTGCATG
    TCCCTTCCTCCAAGAAGCCCGAAGGGAGAATCTGGGAGGGCACTACTGCCCAAGCCCAACCTCCATGTAA
    GCATTACCACAGCTGTGCCCTGAGGAATGGCCCCACGTCACCCTTGCCAGATATTTGTA
  • Protein Sequence : Show Sequence
    >gi|113199771|ref|NP_034944.2| myelin-oligodendrocyte glycoprotein precursor [Mus musculus]
    MACLWSFSWPSCFLSLLLLLLLQLSCSYAGQFRVIGPGYPIRALVGDEAELPCRISPGKNATGMEVGWYR
    SPFSRVVHLYRNGKDQDAEQAPEYRGRTELLKETISEGKVTLRIQNVRFSDEGGYTCFFRDHSYQEEAAM
    ELKVEDPFYWVNPGVLTLIALVPTILLQVSVGLVFLFLQHRLRGKLRAEVENLHRTFDPHFLRVPCWKIT
    LFVIVPVLGPLVALIICYNWLHRRLAGQFLEELRNPF
  • Molecule Role : Protective antigen
3. Mog
  • Gene Name : Mog
  • Sequence Strain (Species/Organism) : Rattus norvegicus
  • NCBI Gene ID : 24558
  • NCBI Protein GI : 158262022
  • Genbank Accession : BX883052
  • Protein Accession : NP_073159
  • Taxonomy ID : 10116
  • Chromosome No : 20
  • Gene Starting Position : 4044676
  • Gene Ending Position : 4054908
  • Gene Strand (Orientation) : +
  • Protein Name : myelin oligodendrocyte glycoprotein
  • Protein pI : 8.13
  • Protein Weight : 25744.92
  • Protein Length : 245
  • DNA Sequence : Show Sequence
    >gi|389675109:4044676-4054908 Rattus norvegicus strain BN/SsNHsdMCW chromosome 20, Rnor_5.0
    AGGCTTCTTGGGGTAAGGGACATGCAGCCAGAGGGCCTTAGCTTGGCCTGATCCTGCTGTCAGGGACCCT
    GTCCCCAGTAACCATAAAGATGGCCGGTGTGTGGAGCCTTTCTCTGCCCAGCTGCCTCCTGTCCCTGCTC
    CTCCTCCTCCAGTTGTCATGCAGCTACGCAGGTAAGCATGCTCCAGCCCTGCAGAAACCCAAGAGCAGAA
    AGGGAGTACCCTTGGCTAACCTCCTCTATTATCCTCAAATGGCTACACGAACTTCCTGTCTACACTTGGG
    TCTGCTGGCACGGCTTCTCTCCTTAGAGACCTTCCTGACTTCTTGGAGAATAAAATCCAAACCTTTCCTC
    ATTCAAATGGTGATGGGAACCAAACTCAGGGCCTGGTGCAAGCTGAGTTCTAAGCTCTATCTATAGCACT
    AACTTCTTGGAGTTACTGTCAGAGTTAAGTGAGAGTGTTTTGGACTTTGCACATTAAGGCCATGCCTGTT
    TGTAGTAAATCTCAGTAAACGTTTGTTGAGGGTTGGTGAGATGGCTCAGTGGGCAAACATGCTTGCCTCT
    TAGACTGGATGCTAACATGAAACAACGCAAATATGAAAAAAATAGAAACACATTGAGTTATAGCACCGTG
    TTATGTTTGAGAACTTGCTCTGCAGAATAAGGCAGTGATACGTTTTTATCCAGGAGGCTGTTCTCCAACG
    AGGAGAAGAAATAAACACTAGACAGAGTTCTACCTCAGGGAGCCATGTGATTGACAGAGCCCTGGCCCAG
    GCACCTTCTGTGGACACCCTAGTACAAGGAAGAACCCAGCTTGCTTGCCAGTAGCCTAATCTCACTAGGG
    AGATGTTCAGAGAAGGTTTCAGAGCTGGGATGAGGTTGAAGATACAAAATGAAAAGAGACTTAATAAATA
    GGGGTTAGAGAGATGGCTCTGTGGTAAAGAGTATTCCTTCTCGTACAGGAGACTGGTTTTTTGGTTACCA
    GTACCCATATCCACGGGGTGGCTCACAACAACCTGTTACTATCTGGGGAGGCAGCTATCCCCAGAGTGTG
    TGACTTCCTCAGTGGAGAATGAGATGTGAGGAGAGAGGGCTGGGAAGCATTTAGAAGCCACATGACAAGG
    AGGAGGCAAGCATCTGAGCTGAAGGGTCAGAGATGAACGTAGCTGATAGACAAGTGGAAGTCACTGTCCA
    GAGGGTTTCATGTTAGAGCCTTCTGTCCTAGACATTCAGAACATATCTTTAAGTAGAGACTGCAAATTTC
    GTATTTGTTTAAAGTTAAAGAAAAAAAAAAAAAAAGCCCTACCGAGGCTCGTGAATTCCGTTTAAAATTC
    CTAAACCTGGCATGGAGGTGAGCCCTTGTAATTGTAGCATTTGTGGGATGGAAGGAAAAGGACCAAGAAT
    TCAAGTCGTTCTCAGCTACGTGGAAGTTTAAGGCCTGCTTGAGCCATGAGCGCCGTGTCTTAAAAACTGA
    AGAGTTGATTCAACAGTTAAGTACGCTTGCTGCTCTTGCAGGGGATCCAAGGTAAGGTTCCCAGCACCAT
    CCTCTATGGGGTAGCTCGAAGCTACCTGTAACCCCAGCTCCAGGGGACCTAACAGCTTTTTACAGATTCT
    AAGAGTAGTCACGCAAAGGTGGCATACAGTCACCCACACACACACACACAGACACACACACACACATATA
    CACACACACACTTTAAAATAAAACAAATCACTGGATATGGTGGTATACAGCTCTGAGAGGCAAGGGCAGG
    CAGATCCCTGTGAGTTCCAGGCTAGCCAAGGCTACGTAGTGTCTCAAAAATAAATAGAATAAAAATGAAA
    AAATCATGTTTCCTTCCCCTCACTCTTTTTGGAGCGGCTTTCCTATGATGGATGAATTTGTCATATCTGA
    TTCTCGCTTTTTTTTTTTCTCCCTCCCCTTTGGTGTCTTGGACAGGACAGTTCAGAGTGATAGGGCCAGG
    GCATCCCATCCGGGCTTTAGTTGGGGATGAAGCAGAACTGCCGTGCCGTATATCTCCTGGGAAGAATGCC
    ACGGGCATGGAGGTGGGGTGGTACCGTTCTCCCTTTTCAAGAGTGGTTCATCTGTACCGAAATGGCAAGG
    ACCAAGACGCAGAGCAAGCGCCTGAATACCGGGGACGCACAGAGCTTCTGAAAGAGTCTATCGGCGAGGG
    AAAGGTTGCCCTCAGGATCCAGAACGTGAGGTTCTCGGATGAAGGAGGCTACACATGCTTCTTCAGAGAC
    CACTCCTACCAAGAAGAAGCCGCCGTGGAGTTGAAAGTAGAAGGTGAGTCCTCCGAAGCCATACGCAGTA
    TGGACTGTGGGGTAGTTGACCTCCCTTCATCCTCCAATCACCCAGCTGAGGTCTTTCTACCCAAAGACCT
    CACTTCCGAGATCGATCCCAAGGGGACAGACGCAACAGTAAACAGGGCTCTTATGCTGTGGCTGTTGGTT
    ATCTCGTTATTTGAATCAGGAAAGAAAACTAGAGTCGAAGCCCGTTCTCACGGCTAAGAACGTGACTTTT
    TGGTATCTAAGGTAGACAAATGCTCCTGCACTTCAGTTTCTGTTTCTGTTTGGTTTTGAAATGATGCCTT
    TTCAACATGTAGCCGCTGGCTGGCCTAGAACTCACCCTATAGACCAGGTTAGTCTCAGACTCAAAATGGT
    TCTCCCGTCTCTGCCTCCTAAGTGCTGGGAGCTGAGATTAAAGGCATGTGATACATACATACATAAATAT
    ATACATACATACATATATATATATTGGGCAATCCTCCAGCCTCGGTCTCCTAAGTGCTGGGACTAAAAGC
    ATGAGTCACCAGCTCAGCTTAAAATGATCGCTTTAGAAACATACCAGATAGCTTGGGTGCTTCCCTCAAG
    AGCTTGTTGAGTAACATAAGCAAGCCATAGAATATAGGGAACAAAAACATATCCCTGTGTTTGAGTGTAT
    AATTACACACACACACACACACACACACACAGTCATATATATATATATATGCACACTCAATATGTATATA
    TGCATATACGTGTGTGGATTCTATGGCAGGGATATAGTGTGTGCGTGTGCGTGTGCGTGTGCGTGTGCGC
    GTGCGCGTGCGCGTGCGCGTGCGCGTGCGTGTGCACGTGCGTGTGCGTGTGCGTGTGCGTGTGCACATGC
    GTGTGCGTGTGCACATGCGTGTGCGTGTGCGTGTGCGTGTGCACATGCGTGTGCGTGTGCGTGCGTGTAA
    TATATGGCAGGGCTAATTCAGTTGGTAGAGTTCTTACCTAGAATGGAAGTAACCATGGGTTCAGTTCCCA
    CCACTGCCTAAACTGATCTTAGAACCTGAAGCCTGTAACCGCGGCTCTTAGAAGTTGAAGTCAAGAGGAT
    GAGAAGTCAGACACTAACCTCCAATATGAAACTGCCTCAAACCAAACCAAACCAAACCAAACCAAACCAA
    ACCAAACCAAACAACCAAACCAAGAGGCTGGGAAGATGGCTCAGTGCTTAGTAGCTTTTGCTGCTCTTGC
    AGAGAGAGGTTCAGAGTTTGGTTCCAGTACCCACATAACCCCAGCTCTGGGGGATCTGACACCTTTTCTG
    GCCTCCAAAGACCAATACCCAACCCCCAAATATAGACCTAAATAAAAATTAAGCACACATACACAGTGGT
    GGAGGAGACCCGTAGCTTCAGGACACTGCTTTGGTTTTTAAGATATTTATTTATTTATTAATTATATATA
    AGTACACCGTAGCTGTCTTCAGACACATCAGATCTCATTACAGATGGTTGTGAGCCACCAGGTGGTAGCT
    GGGAATTGAACTCAGGGCCTCTGGAAGAGCAGTCAGTGCTCTTAACCGCTGAGCCATCTCCCCAGCCCTC
    TGCTTTGTTTTTCTAACTTCATTTTTCAGTGTGCTTTGTGTTGTGCGGTGGCCGACTTTTGGAGTTACTC
    CTTATTATGTGTCCTGCCTCAGAGTGGTTAAAGCATGGATAAGAAAACAGACAGGGAGCGTTGAAAGTTA
    ACCGCGATGTAACTGAAGAACAAAAGTGTTGTGAGCGCCGGGCCTCTGACCCTTTCCCTTCCTCCCCAGC
    ACCTGCAGTGTGAGCATCAGACAATCCATCATCTCTGTTATCTGAGGTGGGGGTGACTGTTGCCAGGCTG
    AGACCAGAAGATGCATTGTATGCCCAGTAACAAAGCGCTATTTCTAGAGACAGCTTGCAGGGACATGAAA
    ATAGCTGTGTGCCACAGAATGTTCTTCAGACAGCTGGTTTTTTTTTTTTTTTTGGTTGGGGAAATTTTAC
    TCACACACACAAAAAAAATGTGGAGGGTCAGAACTTTAGCATACAGATTTTTCTCTCTTCCTGATGAACA
    AATTCCCAGCTCATCTGCCAACTCCAGCACACTCATTGAGACTCAACAAATTGCAAGTAAGAAAGGTGGG
    GGTGGGGGATAATGTGGCAGAACAGGCATTATAAACTGTGCCTTGGGGGAGAATGCAGTGTTTTCCTTCA
    AGTTGGTTTCCACATTCCAAATTCCTCTTGTCAGCTCCCGGTTTGCTTGCCTGACACACACACACATACA
    CACACACACACACAGACACAGAGAGACAGAGACAGAGACGGAGAGACAGAGACAGAGAGAGTGGTTGGAG
    TTCCTTTCAAAACATTGAATAAAATTTGGGGAGTTGTAGCCCAGGCCTAAATACCCAGCATTTGGGACGT
    AGAGGCTGGAGGACAAAGAGTTCAAGGACAGATTTGCCCTGTTTAAAACGAAACAAACAAAAAACAATGG
    CCACATTATCTCTTTTAGAAACAAACATACTTTACCAGCATTTCCATTCTATGTTACCTCCTACCCCTCA
    CCCCCCAATTTTATTGAGACAGGTCTTGCAGGCTGGCCTGTGAGTTGCAGCAATCCTCCTGCCTTAGACT
    CCAGAATGCTGGGATTAGGGGGTATGCGGCACCAGGCCTGGGTGTGTCAGTATTTTTAAAATCTTGATGT
    TGGTTCCTTTATTCAACCCAACCTCCTGTAGCACAGGCTCTGTACAGGGTGTTGAGGAATTGGTCTGCGT
    GATGTGTTCCTTCTGCCTTTCAGATCCCTTCTACTGGATCAACCCTGGCGTGCTGGCTCTCATTGCCCTT
    GTGCCTATGCTGCTCCTGCAGGTCTCTGTAGGCCTTGTATTCCTCTTCCTGCAGCACAGACTGAGAGGTA
    TGGGTCTGTCAGGATCCTTTCCTGACTCCTAGGGACAGTTAATCCTTAAGTCTTCTCAGATCTGAAGTAC
    ATAACAGGTTCTGCTGAACAATCTACCTTGCACCTACTTGTGAAGTAAGACTGACAGCCTCGTTGCTGAG
    GAAGAAATTGTATCGAGGGGTGGTTCAGTGACCTGCTCAGATGACATGTTACAGGCAGTAGAACTCTGCC
    CTTCTAGTCTTTAGCAGCCTCATGAAACTGGGCGAGTAGACAGTCAGATGTAAAATACTACGCATTTGAA
    ATAAATAAACCCTAAGGAAGGGCTGGAGAGATAGCTCCATGGTTAAGAGCAATTGTTCCTCTTCTAGAGG
    ACCCTGAACCCACATCAGGGAGCGTACAGCCATCTGTAACCATGATGTAATAGCCATCTAGCTCCATGGG
    ATCCAACATTTCTGGCTTCCTCAGGCACCTGCAGTCATGTGCATATACCTACATCCAGACACACAATTAA
    AAATTAAATCTTACGATTTTTTAAACTTTCGAATTTATTTTTGTGCATGCTTGTCTATGTGTGCCCATGT
    GTTTATAGGTGCTGTAAGAAGCCGGGAGAGTGTCAGAGACCATGGAACTGGAACTATAGGTGGTCGTTAA
    GGTGCTCAGTTCCAGGCTCTTGTGGTCTTCCATTATTTCATTCAGACAACACCCACAGACAGAGGCTGAA
    TTCTTGGTTTTAAATCTAGAGTTCTTCCTACTCTCCTAAGACACTCCCAGGCCATCAAACAAATCCCCCA
    CACAAAGGTCAATGGTGCTGGGTGTAGAAGGGTCCTCATTTTGTCAGATCAAGAACTAAAAAACCTGCCC
    TTTTCTATTTTAGGAAAACTCCGTGCAGAAGTCGGTGAGTTCCAGGCGCCCTCCCTCCCCGACTCATCTT
    ATCTTTTAGCTTAACCAAAATGTCCTTCTCTTCAGACCATTTCTGCCTCTCCCCCAAAGCTCCGTGCTCT
    CTCCAGTTGGGAACTTTAATATTTAAAACTACAGGTGGCTCAAATTTAGAAGTTGTTTGACCTGAGTTCG
    GTTTACTTTTTTGTCAGGGCTAGAACCAGCCTTGGGGGATTTAGATAGGTCCAGGGAAGGAACTCCCTCC
    CTCTCAGCTCCTTACTGAACCTATTCTTATTTGTCCTTTCAGAGAATCTCCATCGGACTTTTGGTAAGTT
    CAGACATATCCCAACCTTCCCAAGTCACTGTGGGATTTCACTAGTGGCCTGGGGGATGCGGGGGTGGGGT
    GGGGGGTGGAGACTAGGAACAGTGGCTTTTTGTAAGGCTCCCCTATATCTCTTTTCTTTGAATTCAAGAT
    CTACAGGAATGTAGCAAACATTTATGTACCATATTCCAATGTCAGGCCCTGCGTTGAATTTTACAAATTG
    TAAACTTATTATATACATAACTATCTATATCTATCTATATATTATTTTATATATATATATATATATATAT
    ATATATATATATATATATAAAATCTTTCAAATTATAAAAAAGTGGTTAAAGAAATCCTTTGCACATAAAA
    TTGTTTTCCCGATGGGAAAATGAAATATACTTCAATAAGACACCGTCACTGGGCCTAAAGACATGGCTCA
    GTGGTTAAGAGCACCTGTTGCTCCCCTAGAAGACATAAGTTTTTTTCTCCTGACCCCGCAGCAGACAGCT
    GTACGCACAAGGCGTACATATGTAAGAAAAAATAATTTTTTTTTTTTTGAGATATGGTCTTGCAACGTAG
    CCCTGGCTAACCTGGAATTCGCCATGAACGATAGGCTGGCCTTGAACGCACAGAGCTCCACCTGCCTCTG
    CTTCCCCAGTTTTGGGATTAAAGACATGTGCCACCATATCCAGAAAATTACGTTTAAAAAGTCATCTGCT
    ATACTTTTAGGGCAGAATTGGTCTTCAGGAGTAGAGTCCAAGCACACGATTCATCAGGGAAGGTTTCTCT
    GAGGAGAAAGTAAAGCCTCACTGGTTAACTTTTTTTGAATCAGGGTTTCATATGGCTATGCTGTGTGTGT
    CATGATAGCCTTGGGCTCTGATCCTCCTGCCTTCAACTCCCAAGTGCTGATAATGTGCACATTCAACCAT
    GCATGCCCCAACTAGAATATGAATTCCCCAGGAATCAACTTTGCCTTTTATTCACTTCAGATTGCAGAGC
    TCCTAAGGCCACCACCAGCAATACCCAAATGCTTGTGGAATGGATTCCATACAGCTAAAAAGCAACATTA
    GGCAGAGAACGGGAAATCTGTGCTCTGATCAACCTTCCTAAACACAAACAGCCTTTCCTTCTGCAGTCAT
    ATGTAATCCTGTTCAGCTCTCCTGGTACTTGGCACACTCAGTAGGTTCCACCGTAGCACTTTAGTGTACG
    GATGTCACACATGTAGTCTTTGAACACATTTTATTACCCGTGTGTGGAGGTGGGTGACAGAAAGTGCTCA
    CAGAGGTCAGAGGGACAGTAAGGAGGAGTTGATTCTCTCCTTCCCTCGTGTGGGTCTGGGGATCAAACTC
    AAGTTTAACAGCAAGTGCCTTTATCAACTACGCCATCTCCCGGATCCCATCCCATCCCATTTTGTTTTGA
    AGTGTCTGTCTCATGTATTTCAGGCTGGACTCAAACTTGCTATGAAGCCAAAGACTGTCTTGAATTTTGA
    TCCCCCTGCCTGTACCTCCTATATTCGGGGGTTATAGGTGTGAGCCGTACATGCTGTGCTAGGAATGGAA
    CGCAGAGCTCTGTGCAAGCTACAAGAATATAACCCTACAAACCGAGCTGCCTTCCCAGCCCCCAGGAAGG
    GGCTAATACCAGTTTTTGACTTTTCATTTTTGCTATTTTTCTCTTCCCTTCGAGACTTGAACTCAGTAGT
    GTGTCTGAAGACAAATGCAGCCAGCGCACTCATATAAATAATAAATAAATCTTTAATAAAAAGGAAACAT
    TAGTGAAAGCAGGGTTGACAACTTGTAATCCCAGCACCTGGAAGGCGGAGGCAGGAGGACTTCTCAAGTT
    CAAGGCCAGCCAGGGATTGTACAAGCAAGAACCCCTCTTCAAAAAACACGCGCACACACACAAATGCTAG
    AAGTGGGGGCGAGGGAGGGACAGCGTGTGGCTGAAGATCAACAAAGCATGAAAGGGTCACACCACCCATT
    GTCTTCTACGATAAATCGGTTAAAAAGATACAGCTAAATTCAATCAGAAGCTAAAGGAAGTAAAAGCAAG
    GCGCACAATGTACCTCATCTACATTCATGGACTCTACAGCTTGTCCTGGCTTTGGAGGCTTCAAGTGGGT
    GTGGCATGAGCGGCTGTGGCATAGAGGGAAACACTCTGTTCTGCTTTACCATTCAGATCCTCACTTCCTG
    AGAGTGCCCTGCTGGAAGATAACACTGTTTGTTATTGTCCCTGTTCTTGGACCCCTGGTTGCTTTGATCA
    TCTGCTACAACTGGCTGCACCGAAGACTGGCAGGTGCAATGTCTGGGCAGCAAACAGGACCACTGTGGGG
    CGGGGGGGGGGGGAGGGGGGGGAGCTCAGGCAGACCAGAACGGGAAAAGAGTGGAACCAAGGTTCTAGAT
    CCCAGGGCTGAGAATCTTTTTGCTCTCTCTACTCTCACCATCAGAATCCTCAGGAAAACTGAGGACCACT
    TACTGAGATGCACAGGCCCCTTAATGGAAAAGAAGGGTTGTCTGAGGAGGTGGGGGGGAGGCTCCCACTG
    GTCACCCATCGACTCATTTCTCTTTGTTTACAGGACAGTTTCTTGAAGAGCTAAGTAAGTTCTCCCTAAC
    GCCCCTCTCCATTCTTGAGACATCTCATTACGCAGCCCTAGAACTCACTATGTAAACCAGACTGGTCTGG
    AAACTATGGCAATCCTCTTCTCAGCTCCAAAGTGCTAAGGTCATAAGCAAATACCACCGAACCCAAGTGT
    TTTTTTCCTTCTAAACTGATCAAAAATCCAGCAGAGGAACATGGTCACTGGGGGACCATAATCCCAGGGA
    GGGCTGGAATGATTCCTTCTGTGATGGAAAAGGGAAGGGAATTAACTGTTAAGAGACACCACTTTCAGCG
    CCAGAGAAGCTGCTGAGCCACTCCACTCTTACCTGTTGACTTGATAGCCTTTAATGCCCCACAGCCAGAA
    TCCCATTTCTGTAGCCTCCTTTGACATTCTCCCTAATAGTTAGCCTGACTGAACCTCACCTCCCTTCGAT
    AACATGACCCCCGTGAGCTTTCTACCTCCTAGGTTCGATGACTTCAAGAACCCACTGGCAGATTTTTTAA
    GTCACTTGTCTTTCTTTCTAGGAAACCCCTTTTGAGTGATGGTGCATCTTCCTGGAGCGAAGGAAAGCCT
    GGCAGGTCCTTGGGGATGTCTCTCACTGGCCTCTCTGTTATGGACACATTCCAATCTACATCTACAGAAA
    CACTCGGGATCCTGAGTAGAACTTGATTTTCCTCCCACAACTGTCTTTCTTTCCTCAGCTCCTTCCTCTA
    AATCTTTTTTGACTCCTGTCTTAGTCTGCCTCTAGAAAACACCCCATGACTAAGGCCAGGGAGTCTATCA
    GGAGACACCTATGTACCAGAGTAATACAGGAGTGATTCTGCCAACCAGGACACCCAGCGCTTCAACATTA
    CGATCTACAGAACATCAGCTCTGCAAATGGTGGATATTTCTTCCAGAGCCACAGTCTTGACTGGGAAGAA
    TATCAGTGGGGAGAGTAAGGGGGTGAGGGACAGAAGAACCCACAACATGAGGGAAAGCTAAATGAGCCAG
    GGTATGAATATGGGAGCCTGAACCAAAACCTGGAGATTAAAAAAAAAAGTCAAAAGAATACTGACTGGAT
    CAGAGCAACACCATCTTGCTTTGGCAGCCTGGTGGGAGGACCTTGAGAAGCAAGGGTCGAGAACTGGGTC
    TGTTCTCTCATTTTTCTCCTATCTTATTTGAGTACCCACTGTTTGGTTTTTTTTTTTTTTTTCTTCTTCG
    TTTTTTCAACAATTACTACTTGGTGGTGTATCAGACACAAACAAGCTAGGTGCTAATTAATAATAATACA
    AGTTTCTTTGTGC
  • Protein Sequence : Show Sequence
    >gi|158262022|ref|NP_073159.2| myelin-oligodendrocyte glycoprotein precursor [Rattus norvegicus]
    MAGVWSLSLPSCLLSLLLLLQLSCSYAGQFRVIGPGHPIRALVGDEAELPCRISPGKNATGMEVGWYRSP
    FSRVVHLYRNGKDQDAEQAPEYRGRTELLKESIGEGKVALRIQNVRFSDEGGYTCFFRDHSYQEEAAVEL
    KVEDPFYWINPGVLALIALVPMLLLQVSVGLVFLFLQHRLRGKLRAEVENLHRTFDPHFLRVPCWKITLF
    VIVPVLGPLVALIICYNWLHRRLAGQFLEELRNPF
  • Molecule Role : Other
III. Vaccine Information
1. EAE DNA vaccine pZZ/MBP68–85 encoding MBP
a. Vaccine Ontology ID:
VO_0004401
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Rat
e. Gene Engineering of MBP from guinea pig
  • Type: DNA vaccine construction
  • Description: This DNA vaccine expressed an autoantigenic peptide, myelin basic protein peptide 68-85, from guinea pigs (Lobell et al., 1999).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intramuscular injection (i.m.)
g. Rat Response
  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: The pZZ/MBP68–85-treated rats were protected from EAE. The control plasmid pZZ, which does not encode MBP68–85, failed to protect from EAE (Lobell et al., 1999)
IV. References
1. Bourquin et al., 2000: Bourquin C, Iglesias A, Berger T, Wekerle H, Linington C. Myelin oligodendrocyte glycoprotein-DNA vaccination induces antibody-mediated autoaggression in experimental autoimmune encephalomyelitis. European journal of immunology. 2000; 30(12); 3663-3671. [PubMed: 11169409].
2. Lobell et al., 1999: Lobell A, Weissert R, Eltayeb S, Svanholm C, Olsson T, Wigzell H. Presence of CpG DNA and the local cytokine milieu determine the efficacy of suppressive DNA vaccination in experimental autoimmune encephalomyelitis. Journal of immunology (Baltimore, Md. : 1950). 1999; 163(9); 4754-4762. [PubMed: 10528174].
3. Lobell et al., 2003: Lobell A, Weissert R, Eltayeb S, de Graaf KL, Wefer J, Storch MK, Lassmann H, Wigzell H, Olsson T. Suppressive DNA vaccination in myelin oligodendrocyte glycoprotein peptide-induced experimental autoimmune encephalomyelitis involves a T1-biased immune response. Journal of immunology (Baltimore, Md. : 1950). 2003; 170(4); 1806-1813. [PubMed: 12574345].
4. Ramshaw et al., 1997: Ramshaw IA, Fordham SA, Bernard CC, Maguire D, Cowden WB, Willenborg DO. DNA vaccines for the treatment of autoimmune disease. Immunology and cell biology. 1997; 75(4); 409-413. [PubMed: 9315486].
5. Ruiz et al., 1999: Ruiz PJ, Garren H, Ruiz IU, Hirschberg DL, Nguyen LV, Karpuj MV, Cooper MT, Mitchell DJ, Fathman CG, Steinman L. Suppressive immunization with DNA encoding a self-peptide prevents autoimmune disease: modulation of T cell costimulation. Journal of immunology (Baltimore, Md. : 1950). 1999; 162(6); 3336-3341. [PubMed: 10092787].
6. Selmaj et al., 2000: Selmaj K, Kowal C, Walczak A, Nowicka J, Raine CS. Naked DNA vaccination differentially modulates autoimmune responses in experimental autoimmune encephalomyelitis. Journal of neuroimmunology. 2000; 111(1-2); 34-44. [PubMed: 11063819].
7. Tsunoda et al., 1998: Tsunoda I, Kuang LQ, Tolley ND, Whitton JL, Fujinami RS. Enhancement of experimental allergic encephalomyelitis (EAE) by DNA immunization with myelin proteolipid protein (PLP) plasmid DNA. Journal of neuropathology and experimental neurology. 1998; 57(8); 758-767. [PubMed: 9720491].
8. Waisman et al., 1996: Waisman A, Ruiz PJ, Hirschberg DL, Gelman A, Oksenberg JR, Brocke S, Mor F, Cohen IR, Steinman L. Suppressive vaccination with DNA encoding a variable region gene of the T-cell receptor prevents autoimmune encephalomyelitis and activates Th2 immunity. Nature medicine. 1996; 2(8); 899-905. [PubMed: 8705860].
9. Walczak et al., 2004: Walczak A, Szymanska B, Selmaj K. Differential prevention of experimental autoimmune encephalomyelitis with antigen-specific DNA vaccination. Clinical neurology and neurosurgery. 2004; 106(3); 241-245. [PubMed: 15177776].
10. Weissert et al., 2000: Weissert R, Lobell A, de Graaf KL, Eltayeb SY, Andersson R, Olsson T, Wigzell H. Protective DNA vaccination against organ-specific autoimmunity is highly specific and discriminates between single amino acid substitutions in the peptide autoantigen. Proceedings of the National Academy of Sciences of the United States of America. 2000; 97(4); 1689-1694. [PubMed: 10677519].
11. Wiki: Experimental autoimmune encephalomyelitis: Experimental autoimmune encephalomyelitis [http://en.wikipedia.org/wiki/Experimental_autoimmune_encephalomyelitis]