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Arthritis

Table of Contents
  1. General Information
    1. Introduction
  2. Vaccine Related Pathogen Genes
    1. COL2A1 (Protective antigen)
    2. Collagen type II (Protective antigen)
    3. Collagen type II (Protective antigen)
    4. hsp65 from M. leprae (Protective antigen)
  3. Vaccine Related Host Genes
    1. Ifng (Interferon gamma)
    2. IgG (partial)
    3. Il10 from Rattus norvegicus
    4. TGF-B from Rattus norvegicus
    5. TNF-alpha
    6. TNF-alpha from Rattus norvegicus
  4. Vaccine Information
    1. Adjuvant arthritis DNA vaccine CII encoding type II collagen
    2. Adjuvant arthritis DNA vaccine pcDNA-CCOL2A1 encoding type II collagen
    3. Adjuvant arthritis DNA vaccine pCMV3.65 encoding HSP65
    4. Pristane-induced arthritis DNA vaccine DNA-hsp65 encoding Mycobacterium leprae 65-kDa heat shock protein
  5. References
I. General Information
1. Introduction
Arthritis is inflammation of one or more of your joints. The main symptoms of arthritis are joint pain and stiffness, which typically worsen with age. The two most common types of arthritis are osteoarthritis and rheumatoid arthritis. Osteoarthritis is usually caused by normal wear and tear, while rheumatoid arthritis is an autoimmune disorder. Other types of arthritis can be caused by uric acid crystals, infections or even an underlying disease, such as psoriasis or lupus (Mayo Clinic - Arthritis).
1. COL2A1
  • Gene Name : COL2A1
  • Sequence Strain (Species/Organism) : Gallus gallus
  • NCBI Nucleotide GI : 15546069
  • NCBI Protein GI : 15546070
  • Protein Accession : AAK98621.1
  • Taxonomy ID : 9031
  • Gene Strand (Orientation) : ?
  • Protein Name : alpha 1 type IIA collagen precursor
  • Protein Note : breed: San-Huang
  • DNA Sequence : Show Sequence
    >gi|15546069|gb|AY046949.1| Gallus gallus alpha 1 type IIA collagen precursor (COL2A1) mRNA, complete cds, alternatively spliced
    ACTGCTCGGCCCGACGGACGGTGAGAGGGCCTCCGCCGCCCGCCATGCACGGCCGCCGCCCGCCCCGCTC
    CGCCGCTCTCCTCCTCCTCCTCCTCCTTCTCACGGCCGCCGCAGCCGCGCAGGACCGCGACCTCCGACAA
    CCTGGCCCCAAGGGACAGAAGGGAGAACCCGGAGATATTAAAGATGTTGTAGGACCCCGAGGGCCTCCAG
    GACCACAGGGCCCAGCAGGAGAGCAGGGACAGCGAGGGGACCGTGGCGAGAAGGGGGAGAAGGGTGCTCC
    TGGCCCCCGTGGGAGGGATGGAGAACCCGGCACCCCTGGAAACCCAGGCCCCCCCGGTCCCCCCGGACCT
    CCTGGCCCCCCCGGACTTGGTGGAAACTTTGCGGCGCAGATGGCGGGCGGCTTCGATGAGAAGGCGGGTG
    GAGCGCAGATGGGTGTCATGCAGGGACCCATGGGCCCTATGGGACCCCGCGGCCCCCCTGGCCCCACTGG
    CGCACCTGGTCCCCAGGGATTTCAAGGCAACCCCGGTGAGCCCGGCGAACCCGGCGCTGCTGGTCCGATG
    GGTCCCCGGGGACCTCCGGGACCACCTGGGAAACCCGGTGACGATGGTGAGACAGGCAAACCCGGCAAAT
    CTGGTGAACGTGGCCCCCCCGGCCCCCAGGGCGCTCGTGGCTTCCCTGGGACTCCTGGTCTCCCCGGAGT
    GAAGGGCCACCGAGGCTACCCCGGTTTGGATGGTGCCAAAGGAGAGGCGGGGGCTCCTGGAGCCAAGGGT
    GAATCTGGTTCACCGGGTGAGAACGGCTCCCCCGGCCCCATGGGACCCCGTGGGCTGCCCGGAGAGCGAG
    GACGTCCCGGCCCCTCCGGCGCCGCCGGTGCTCGTGGCAATGACGGTCTCCCTGGCCCTGCTGGACCCCC
    TGGACCCGTCGGCCCTGCCGGAGCCCCCGGCTTCCCCGGAGCCCCCGGTTCAAAGGGTGAAGCCGGCCCC
    ACTGGTGCACGGGGTCCCGAGGGTGCCCAAGGACCCCGCGGCGAATCCGGCACCCCCGGCTCTCCCGGCC
    CCGCTGGCGCACCCGGTAACCCAGGGACTGATGGCATCCCCGGTGCCAAGGGCTCGGCGGGTGCCCCGGG
    CATTGCAGGCGCTCCAGGATTCCCCGGCCCACGCGGCCCCCCCGGACCCCAAGGTGCCACCGGACCACTG
    GGACCCAAAGGACAGACGGGCGAACCCGGCATCGCAGGCTTCAAGGGCGAGCAAGGACCGAAGGGCGAGA
    CGGGCCCCGCAGGACCCCAAGGTGCCCCCGGGCCGGCTGGTGAGGAAGGCAAGAGAGGAGCTCGTGGTGA
    ACCTGGTGCCGCCGGCCCTGTGGGCCCCCCCGGAGAAAGGGGCGCTCCTGGCAACCGTGGATTCCCCGGG
    CAGGACGGGCTGGCCGGACCCAAGGGTGCTCCAGGTGAACGCGGCCCCGCTGGTCTCGCCGGTCCCAAAG
    GTGCCACCGGTGACCCCGGACGTCCCGGAGAGCCCGGGCTGCCCGGAGCGAGGGGTCTCACCGGCCGCCC
    CGGCGATGCGGGACCTCAAGGCAAAGTCGGCCCAACTGGTGCTCCTGGCGAGGATGGCCGCCCCGGCCCC
    CCCGGACCTCAGGGTGCTCGTGGGCAGCCTGGTGTGATGGGTTTCCCCGGTCCCAAAGGCGCTAATGGTG
    AGCCTGGAAAAGCTGGAGAGAAAGGACTGCCCGGCGCCCCAGGGCTGCGGGGTCTGCCTGGCAAGGATGG
    GGAGACGGGAGCTGCCGGCCCCCCTGGACCCGCTGGTCCTGTGGGTGAGAGAGGAGAGCAAGGAGCCCCC
    GGTCCTTCCGGCTTCCAGGGACTGCCCGGACCACCAGGTCCCCCTGGGGAGAGCGGCAAACCCGGAGACC
    AGGGTGTTCCTGGAGAAGCCGGTGCCCCCGGTCTTGTTGGTCCCAGAGGTGAACGTGGATTCCCCGGTGA
    ACGCGGCTCTCCCGGTGCCCAAGGGCTGCAGGGTCCCCGTGGGCTCCCCGGAACGCCCGGCACTGACGGA
    CCCAAGGGTGCAACCGGTCCAGCCGGCCCCAACGGTGCCCAGGGTCCCCCAGGGCTGCAGGGAATGCCCG
    GTGAGAGAGGAGCAGCTGGCATCGCTGGCCTCAAGGGTGACCGGGGAGATGTTGGTGAGAAAGGACCTGA
    GGGAGCTCCAGGCAAGGATGGCGCACGTGGTCTGACGGGTCCCATTGGTCCCCCTGGCCCTGCTGGCCCC
    AACGGTGAGAAGGGTGAATCCGGCCCTCCTGGTCCATCTGGTGCTGCCGGTGCCCGTGGTGCCCCCGGTG
    AGCGTGGCGAGCCCGGTGCCCCCGGTCCTGCTGGATTTGCTGGCCCCCCGGGCGCCGATGGACAACCCGG
    TGCCAAAGGCGAGCAGGGAGAGCCCGGGCAGAAGGGTGACGCGGGCGCTCCTGGTCCCCAAGGTCCCTCC
    GGCGCTCCTGGCCCCCAGGGCCCAACCGGTGTCACTGGTCCCAAAGGAGCTCGTGGGGCTCAGGGTCCCC
    CTGGAGCCACGGGATTCCCCGGAGCTGCCGGCCGTGTGGGACCGCCCGGCCCTAATGGTAACCCAGGCCC
    CCCCGGACCCCCTGGCTCTGCTGGCAAGGACGGCCCCAAGGGTGTTCGTGGCGACGCCGGCCCCCCCGGC
    CGTGCAGGTGACCCCGGCCTCCAAGGCCCCGCCGGCCCCCCCGGCGAGAAGGGCGAACCCGGCGAGGACG
    GCCCCGCGGGTCCCGACGGCCCCCCCGGCCCTCAAGGCTTGGCAGGACAGCGTGGTATTGTGGGTCTCCC
    AGGACAGCGTGGTGAGAGAGGCTTCCCCGGACTGCCGGGGCCATCGGGAGAACCTGGAAAGCAAGGAGCG
    CCTGGCTCTGCGGGTGACCGAGGTCCCCCCGGCCCCGTGGGGCCCCCTGGGCTGACGGGTCCTGCTGGAG
    AACCCGGGCGCGAGGGCAACCCTGGTGCTGACGGTCTCCCAGGCAGGGATGGCGCAGCTGGCGTGAAGGG
    TGATCGTGGTGAGACCGGCCCTGTGGGTGCCCCCGGTGCTCCTGGAGCCCCTGGCGCCCCCGGCCCTGTT
    GGTCCCACTGGAAAACAAGGAGACAGAGGCGAGACGGGTGCACAAGGGCCCATGGGTCCCTCTGGTCCCG
    CTGGAGCTCGAGGAATGCCGGGTCCCCAAGGACCTCGTGGTGACAAAGGTGAGACGGGAGAGGCTGGAGA
    GAGAGGGCTGAAGGGCCACCGTGGCTTCACCGGTCTGCAGGGTCTGCCCGGACCACCCGGCCCGTCTGGA
    GACCAAGGTGCTGCCGGTCCCGCTGGTCCCTCCGGTCCCAGAGGTCCCCCTGGTCCCGTCGGCCCCTCTG
    GCAAAGATGGCTCTAACGGCATGCCCGGCCCCATCGGTCCTCCCGGTCCCCGTGGACGGAGTGGTGAACC
    CGGCCCTGCGGGTCCTCCTGGAAACCCCGGTCCTCCCGGTCCTCCTGGCCCCCCCGGCACCGGCATCGAC
    ATGTCTGCTTTTGCTGGACTGGGTCAGACGGAGAAGGGCCCCGACCCCATCCGCTACATGAGGGCAGACG
    AGGCGGCCGGAGGGCTGCGGCAGCACGACGTGGAGGTGGATGCCACCCTCAAATCCCTCAACAATCAGAT
    TGAGAGCATCCGCAGCCCCGAGGGCTCCAAGAAGAACCCTGCCAGGACCTGCCGCGACATCAAACTCTGC
    CATCCCGAGTGGAAGAGCGGAGATTACTGGATTGACCCGAACCAGGGCTGCACCTTGGACGCCATCAAAG
    TATTCTGCAACATGGAGACGGGCGAGACCTGCGTCTACCCGACCCCCAGCAGCATCCCCAGGAAGAACTG
    GTGGACCAGCAAGACGAAAGACAAGAAGCACGTCTGGTTTGCAGAGACCATCAACGGCGGTTTCCACTTC
    AGCTACGGCGATGAGAACCTGTCCCCCAACACCGCCAGCATCCAGATGACCTTCCTGCGCCTCCTGTCCA
    CCGAGGGCTCCCAGAACGTCACCTACCACTGCAAGAACAGCATCGCCTACATGGACGAGGAGACGGGCAA
    CCTGAAGAAAGCCATCCTCATCCAGGGATCCAACGACGTGGAGATCAGAGCCGAGGGCAACAGCAGGTTC
    ACCTACAGCGTCTTGGAGGACGGCTGCACGAAACACACTGGCAAATGGGGCAAGACGGTGATCGAGTACC
    GGTTGCAGAAGACCTCGCGCCTGTCCATTGTAGATACTGCACCTATGGACATTGGCGGAGCCGATCAGGA
    GTTTGGCGTGGATATTGGCCCAGTCTGCTTCTTGTAAAAAGGGTTGTTGTTATTTGTGTGTTTGTTTGTT
    GTTTGGTTGTTGTTTTTTGTTTCTTTTTTTTTTTTTTTTAGAAAAGAAAGGAATCCAGCCCAATCCCATA
    AAAGCAAACCAGTCCCACCCCCAGGACCCGCACGTTCCCAGCACAACTTCTGCACTGAACGGATGGCACG
    ACCCCGCGCCCCTTCGGGACCCTCCGGCGCCGTCACCGGGCAGACTGCGAAATACAACCACGGGCTTATA
    TTTATTTATTGCCTTCCTGGAAGGCCTGGTTTCGTAGGGCGGGTGGAGGTGGGAATCAATCTGGCAGGTG
    TGACGGCCCCCCTCCCCACAAAGGGATCTGGCAAACGCAGGTATCGCGAATCCCCTCCCCTCCCCGTGTA
    TCACCAGCAGGAGTGCTAATGTATCATACAACAGAAATGGTGCTATTCTTGTAAAACAAGTCTGTATTTT
    TTAACATCAGTTGATATAAAAACAACAAAAAAAAAAACTTTTGGTGGAAAGTAAAAAAAACAAAAAAAAA
    AAAAAAA
  • Molecule Role : Protective antigen
2. Collagen type II
  • Gene Name : Collagen type II
  • Sequence Strain (Species/Organism) : Gallus gallus
  • NCBI Gene ID : 395069
  • NCBI Protein GI : 45383309
  • Genbank Accession : AF452711
  • Protein Accession : NP_989757
  • Taxonomy ID : 9031
  • Chromosome No : Un
  • Gene Starting Position : 72263
  • Gene Ending Position : 86119
  • Gene Strand (Orientation) : +
  • Protein Name : collagen, type II, alpha 1
  • Protein pI : 8.52
  • Protein Weight : 131719.18
  • Protein Length : 1420
  • DNA Sequence : Show Sequence
    >gi|358479026:72263-86119 Gallus gallus breed Red Jungle fowl, inbred line UCD001 unplaced genomic scaffold, Gallus_gallus-4.0 ChrUn_7180000966168, whole genome shotgun sequence
    GAACTTTGCGGCGCAGATGGCGGGCGGCTTCGATGAGAAGGCGGGTGGAGCGCAGATGGGTGTCATGCAG
    GGACCCATGGTAAAGCCTCCCCCTCCCCAAACCCCCCTCCCACCCCAAAACCAGGAGGCCCTCCAGAATT
    CACACCCCTCTCCCCCCCAGAAACTCAACCCAAACCCACACCAAGGGGTCTCATCCCCTCAGCCCCATTG
    TGGGACATGGGGGACCCCACAGCACAGCCGCCATTCTGGGGAGGGGTACCCTCTGTCTCTGCAGTCTGGG
    GTGGGGGTGTGATGGTGGTGAGAGCAACACCCCCCCCCCCCCCATCTCCCCCATAAAACACCCCAAACTC
    ACACCTGGGAGTCCCATAGGGGACCCTGTGCCATCACCACTGTTCTCAGGGGGCACCCTCTGATTTTTTG
    GGGGGAGGCGATGGTGTTGATGGCCGCCTCGCTTCCCCTCCCCCCAAAAAAACCCCACAACCCAACAACA
    CACACCTGGGGGTCTCATCCCTACTGTGGGACATAGGGGGTGTCTCATCCCCCCAGCCCCACTGTGGGAC
    AAAGAGGACCTGCAGCATCACCACCACTTGGGGGGGGGCACCCTCTGTTTTGGGTGGTGGGGGGGGGGTG
    ATGGTGATGACAGCCACCTCAACCCCTCCCCCATGGTGACACTGTCCCTCATCTCTCTCCCTACAGGGCC
    CTATGGGACCCCGCGGCCCCCTGGCCCCACTGGCGCACCTGTAAGTACCCGCAGCCCCCCTGGGCTCCGG
    GGGGCTCCGGTTGGGTGTCCCACCGCCCCCCCAATGCCACCCCCTGTGCTCTCTCCCCACTTTAGGGTCC
    CCAGGGATTTCAAGGCAACCCCGGTGAGCCCGGCGAACCCGGCGCTGCTGTGAGTGTCCCCTCCTTGTCC
    CCACTCTGCCCCCCTTTGCGTCCCCCCTCAACTTGGGGACGTGTCTCCTGCCCTCCTCCCCCCTCCCCCC
    CCCACCTCACCGCTGTCTCTTTGTCCCCTGTAGGGTCCGATGGGTCCCCGGGGACCTCCGGGACCACCTG
    GGAAACCCGGTGACGATGTGAGTCCCCCCGTAACAGCGGTGCTGAGCACGAGCAGCTCGGTGCACTGATA
    CCCCCCTTTCGAGCGCTGCTTCCTCTCCATTAAAACCCCATTATTTCCCTGCAGGGTGAGACAGGCAAAC
    CCGGCAAATCTGGTGAACGTGGCCCCCCCGGCCCCCAGGTGAGGCAGCACTGGGCTGAACGTTGCCCCCA
    TCGCTGCCCCCAACCCTTTTTCCCCTCCCCACCTCAAGTTCACCCTAACCCTATTTCTCTCCACAGGGCG
    CTCGTGGCTTCCCTGGGACTCCTGGTCTCCCCGGAGTGAAAGGCCACCGAGTGAGTGCCCCCCACCCACA
    CCGTCCCCCCATCACTTATATCCCACCGTGCCCCCTCATAACGTTGTTTCTGCCTCTCTCCCTTCACCCC
    CAACCAGGGCTACCCCGGTTTGGATGGTGCCAAAGGAGAGGCGGGGGCTCCTGGAGCCAAGGTGAGTGGG
    GGGCGACCGCCTCCATGGCGTGGGGATGTGTACCCCAACCTGGGGCTCATCCTCTCTCGACCCTTTTTCC
    CATACAGGGTGAATCTGGTTCGCCGGGTGAGAACGGCTCCCCCGGCCCCATGGTGAGTGCGGGGGGTGGG
    CATAGGGGAAACTGAGGCACAGAATATGAGGACGGACCCCATAACCTCTGCGCTGAGCCCAACCCAACAC
    TGTGCCCCCTCCACCCCCGCAGGGACCCCGTGGGCTGCCCGGAGAGCGAGGACGTCCCGGCCCCTCCGGC
    GCCGCCGTGAGTGTGACAGTGGCCCTATGGCACAGGGCTGCGTGTGGGGGGGGATCGGGGTGCTGTGACC
    CCCCCCATGACTGCCGTCTCCCCCCAGGGTGCTCGTGGCAATGACGGTCTCCCTGGCCCTGCTGGACCCC
    CTGTAAGTATAGGGCTGCTTTGACCCCACACCTCAACTTTCTGCTGAACCCCGTAGGGGTTCTCCGGGAG
    CCCCCCCAGCCTGGGCAGCCCCCCCACACTCTCCTGTTCCCCCAGGGACCCGTCGGCCCTGCCGGAGCCC
    CCGGCTTCCCCGGAGCCCCCGGTTCAAAGGTAAGGGGTGAGCAGAGGGACGTGGGGAGGACTGGGGGGGG
    GAAACTGAGGCGCAGTGCCCCGTCTCACCCCACTTCTCTCCCCCCAGGGTGAAGCCGGCCCCACTNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNTCACTGTGAGGATGGCTGGGGGGGCAAGGGAGGATGATGAGGGATGTGGGGATGCTGGGG
    GGTGTGTGGGAGTGCTGGGAGCCATGGAGTCATGGGGGGGGCCATGGGGCCGTGCCGTGCTCCCCCTGAC
    GGCTGCTGTGTGCCTGCAGGGTGCCCCGGGCATTGCAGGCGCTCCAGGATTCCCCGGCCCACGCGGCCCC
    CCGGACCCCAAGGTGCCACCGGACCACTGGGACCCAAAGGACAGACGGTAAGAGACCCCCTGAGACCCCC
    ACGTGGGGACCCCGGGTATGGCCCCGCCCACTGCCAGGTGGCCCCGCCCATACTGGAGGTAGCCCCGCCC
    ACTTTGCCCCAGGCCCCGCCCACCGTGCCGCAGGATGCGCCCCCTGCAGGACCCCAATGTGGGGCACTCT
    GTGTCCCCTCCCCAAAGTGCCCCATGGTCACTCCCAGGAGACCCTCTTCTGGTCCCCAAAGGGGCCGACT
    GGGGATGGGATGGGTGATGAGGTTGGGGTGGGATGGGAATGGGATGAGATGAGATGGGAATGTGACGGTG
    ATGGGGATGAGATGGGCACGTGGGTGGGTATAGGGACAGGAAAGAGGATGAGGAAAGGGCTGGGGACATG
    GATGGTGGTGGGGAGGAGGATAGGGTTGAGGATATGGGAAATGGGGGATGGGAAAGGGGATGGGGCTGAA
    GAATAAGAGGGAGATTAAGAAGGGGGCAGCTGTGGTTAGGGATTGAGGTGGGGATGGGGACACAGCTGGG
    AATAGATGGAGGCAGGAATGGGAGGGGTGTGCAGGGGAAAAGGGATGGAGGATACAGGTGGGGATGGAGT
    GGGAATAGAGATGGGAGTGAGACTGTAGGGCAGGGAAAGGGATGGGGGACAGTGACGGTGTGGGGACAGA
    GATGGAATGGGGACAGGGATTTGATGGGGACAAGGATGCAGATAGGGGTGAAGGTAGGGGTAGGGGCAGG
    AAAAATGCTGGAGAGATGGGGACGGGACGGAATTGGGATAGAAATGGGGTGGGGTGGGATGGGAATGAGG
    CCGGGAATGGGAATGGGAATAGGGCTGGGGTTGGGATGGGGACCGCGATGGGGGCACTGAGAGCCCATTG
    CCCACCGCCCTCTCTTCCCCCAGGGCGAACCCGGCATCGCAGGCTTCAAGGGCGAGCAAGGACCGAAGGG
    CGAGACGGTGAGTTCTGGGGGGCCCATCCAGCCCCCCCCCCATCCGGGGAGGACCCCGCTGCGGGGCTGC
    GTCTCTAACGAACCTCTTCCCCCTAACGAGGCCCCTCGAGGGGCAGCATCCCCCCCTCCTGCAGCGGGGG
    GTGGAGGGGGAACAATAGGCTCTGTGTTAGGGCCGCGCTGTGCCCCCCCCCTCGGCCCCGGGGCCNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGCTGCGAACTGAACCCCAACCCCCACCCGTCCCGGTTTG
    CTGCTCTGACCCCTTTTCCTCCTCTTCCTCAGGGCCCCGCAGGACCCCAAGGTGCCCCCGGGCCGGCTGG
    CGAGGAAGGCAAGAGAGGAGCTCGTGGTGAACCTGGTGCCGCCGGCCCTGTGGGCCCCCCCGGAGAAAGG
    GTGTGTGCAGAGCGCTGCCCCCCACCCCGAAAAAGACAAAAACCCCACAGCCAGCCCTGACTTTGCCCCT
    CTTCTTCTTCTTCCATAGGGCGCTCCTGGCAACCGTGGATTCCCCGGGCAGGACGGGCTGGCCGGACCCA
    AGGTGGGACTTCGGGGTTATGGGGGGGGGTCCTATAGGGAGTGCCGACTCCCTGTGCCCCCCTCCAACCT
    GTGCCCCCCTCCCCCCCACACAGGGTGCTCCAGGTGAACGCGGCCCCGCTGGTCTTGCCGGTCCCAAAGG
    TGCCACCGGTGACCCCGGACGTCCCGGAGAGCCCGGGCTGCCCGGAGCGAGGGTGAGAGCTGGGGGGGAG
    TCCCCATGGGGGGGTGGGGTGCTGGGGGGCTCAGGTGGGGCAGAGGGATGTGGGGTGGTGGTGGTGGGGG
    CTGCTGGGGTCAGCAAGGAGGGGTTTGGGGAGCAGTTCAGGGGGTTTTGGGGTTGGTTAAGGAGCATTTT
    GGGTTTGTTAAGGAGCATTTGGGGTTGGTTAGGGGACGTTTGAGGTTTGTTACAGAACATTTGGGGTTGG
    TTAGGGAGCTTTGGGGGGTTGATGCCTTTGGGGTTGCTTCCTTTGGTTTCAGCCACGAAGCGCTTTGCCA
    TGAAGGTGCTCTTTGGGGTTGGTTCAGGTGCTGCTGGGCTCTGATCAGGATGCACTTTGGCATCAGCTGA
    GATGCACGTAGGGGTGGTTACGGTGCAGGTGGGGCTCATTGTGGTGCATTAGGGGTGGGGGTGCTCCTTG
    GTGCTCATTAACGTTGTCTGTGGGGTGAGTTAGGATGCATTTGGGATGAGTTAAGTTGCTTTTGGAGTTA
    CCAAAGGTGCTCCCTGGGGTTACTAAAGATGCTCTTTAAGGGCACGCAGCCCCCCGTGCCCTCACCCGGC
    CCATTTCAGCCCTGGAGCTGGGCAGGGCAGGGGTTCCCATTGTATCCCCCTCCCAACTGGGGGCCACCCG
    CTGCAGCCCCCACTGCTTCCTTCCCCTGCAGGGTCTCACCGGCCGCCCTGGCGATGCGGGACCTCAAGGC
    AAAGTCGGCCCAACTGTAAGTGATGCTCTATGGGGGTGGGGGAAGGAGGGCTGGGGGTGGCTGTTGTGAC
    CCACGCCGATGTTCCCCTCCCCATGTAGGGTGCTCCTGGCGAGGATGGCCGCCCCGGCCCCCCCGGCCCT
    CAGGGTGCTCGTGGGCAGCCCGGTGTGATGGGTTTCCCCGGTCCCAAAGGCGCTAATGTGAGTATTGACC
    ATTTTCTGCCCCAAAATGGAGCTGTGAAGGTCAGCGCAGCTGTGAGCCCATCCCGTCTCCTCTCTGTAGG
    GTGAGCCTGGAAAAGCTGGAGAGAAAGGACTGCCCGGCGCTCCAGGGCTGCGGGTGAGCTCCTTCTGCCA
    CTCTGACCCCATAAAATCACCTTGTGGGGTTTCCCCTGCACTGTGCCCTGTATGTGGATGGGGAAACTGA
    GGCACGGGGCTGAGTGCCGGGGATTTCTCCTCTGCAGGGTCTGCCTGGCAAGGATGGGGAGACGGGAGCT
    GCCGGCCCCCCTGGACCCGCTGTGAGTGTCCCTTCTTTTCCTCTTGGGGACAGATGTTTGAAATGTGGGG
    CTGGGGGGTGTAGCCCCCAGATCTGGGTGGAAGACCAGCCCAGGAGTGCTGGAGATACGGGGTGGGCAAA
    AGCCCCGGTGTTGAAGTGTGGGGATTTGGTGTCCCAGCCGCCCCATCCCGGTGTGATTTGGATTTTGCCC
    AAAACCTTGGGGTGGGTGAGGGGCTGCAAAGGGGCGAATCCAGGAGGGAGGAGGGATCTCAGAGCTGTCA
    CTGCTGTCCTCCCTTTAGGGTCCTGTGGGTGAGAGAGGAGAGCAAGGAGCCCCCGGTCCTTCCGGCTTCC
    AGGTGAGTGGGGACACACAAGGAGGGTTGGGGTGATGCAGGTGGGCACCCCCCCCTAACACCACTCCTTG
    TCCCCAAACAGGGACTGCCCGGACCACCAGGTCCCCCTGGGGAGAGCGGCAAACCCGGAGACCAGGTGAG
    GCCATTCATCCCTAGGCTGCAAGTGGGGGCTTAGCCTGTGCTGACCCCCCCCCCNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNGTCGCGCTGACCCCCCCCAGTGCTGACCCCTGGCTCTTCCCTCCTCTCTGCAGGGT
    GTTCCTGGAGAAGCCGGTGCCCCCGGTCTTGTTGGTCCCAGAGTGAGTATTGCTCATCCCCCCCCCGCTC
    CTCCTGTCCCACTGAAGGGTTTGGGGGCTCAGGGGGAGCAGAATCCAACCACCCCCACCAAAGCCATCGG
    ACCCCCACAGTTCCAGCAGCTCAGTGTCCCCCCTCTCCTCCCAGTTCACCCCAATCCCACCTGGGATGGA
    TGCGCTGCCCTCTGTCCTGAGGACAGTGCCATTGTGTTCCTCACTATGGCCTCGGGGGGCTTCTCTCTGA
    CCGTCTCCCCCTCTCCCCCCCATAGGGTGAACGTGGATTCCCCGGTGAACGCGGCTCTCCCGGTGCCCAA
    GGGCTGCAGGGTCCCCGTGGGCTCCCCGGAACGCCCGGCACTGACGGACCCAAGGTGGGTGACAGAGGAT
    GGGTGGCACAGTGCCACCACCATGGAGCCGTGATGCCATCGCGGCCCCAATGACCTCATCACTGTTCTGC
    TTTTAGGGTGCAACCGGTCCAGCCGGCCCCAACGGTGCCCAGGGTCCCCCAGGGCTGCAGGGAATGCCCG
    GTGAGAGAGGAGCAGCTGGCATCGCTGGCCCCAAGGGTGACCGGGTAAGGTGATGCGTCCCATAAGTGTC
    CTGAGTGACATCGGGTCTCAGCTCGGCTCACACTCATCCCATCCCACAGGGAGATGTTGGTGAGAAAGGA
    CCTGAGGGAGCTCCAGGCAAGGATGGCGCACGTGTAAGTGGGGCACGGCCATGGGGTGGGCTGGCAAAGG
    ATGCTCACAGAGACCACATCCTCATCTCTCTCTCTCTCCCATAGGGTCTGACGGGTCCCATTGGTCCCCC
    TGGCCCTGCTGGCCCCAACGGTGAGAAGGTGAGAGCAGCATCACAGCACCCCACATTACGCCCCATGGGA
    TGACCCCAGTGCCTCCACCTCTCCATCCTTTCTTTTCCAGGGTGAATCCGGCCCTCCTGGTCCATCTGGT
    GCTGCCGGTGCCCGTGGTGCCCCCGTAAGCACAATGTCTGCAGCCCCTGGGTGCCCCTAACCTTCACCCT
    AAACCCCCATCAACCCCTTTATCAACCTCCCCCATCTCTTCCCATTAGGGTGAGCGTGGCGAGCCCGGTG
    CCCCCGGTCCTGCTGGATTTGCTGGCCCCCCGGTGAGTGTTTCACCCCGAAGCCCCCATCGCACACCCAC
    GTCTTCACCCCACATCCTCACCCCACTCATGGTGGCTGCTGTTCCCATCAGGGCGCCGATGGACAACCCG
    GTGCCAAAGGCGAGCAGGGAGAGCCCGGGCAGAAGGGTGACGCGGGCGCTCCTGGTCCCCAAGGTCCCTC
    CGGCGCTCCTGGCCCCCAGGTACAACCCCAAATGGGGCAAACCCCCAAATTTGGGACGTCACGGCCCCAA
    TGCAGGCACACTGCAGCTCCCGTTCGGATTTGTAACCTGTTTTTCTCTCCTTCCTAGGGTCCAACCGGTG
    TCACTGGTCCCAAAGGAGCTCGTGGGGCTCAGGGTCCCCCTGTGAGTACCGGGGGGTGGGCTGCAGGGTG
    GGGAAGGAACGGCCGTGGGGCTGAGCTGTGTCTGAGCCGTTTCTCCTCTCCCTCTCTCCTCTGACTCTGT
    GATTCCCTCCCCAGGGAGCCACGGGATTCCCCGGAGCTGCCGGCCGTGTGGGACCGCCCGGCCCTAATGT
    GAGTCTGGGGGCGTTCTGGGATTGCCCCCACCTGGGGTTTGGGTGCTGCTTCTCCGTGCTGCGTGTTGGA
    GGGGGCACTGTTTCCCTGCACAGACACGTGGGGTTTTCCTCCTTGGCTCTCTGATGTTGGCTTTTGGGGC
    CATTCCAGTGGTAGAGAAGGACTTTTCTAAGGGCAAGAGCTCCCCAAGAAGCAGCAGTGGGATGCGGGTG
    ATAAAGATGGAATGGCTGCCTCTGGTTTGCACCAGCGCTGCTTTCCTTCCCTTTAGGGTAACCCAGGCCC
    CCCCGGACCCCCTGGCTCTGCTGGCAAAGACGGCCCCAAGGGTGTTCGTGGCGACGCCGGCCCCCCCGGC
    CGTGCAGGTGACCCCGGCCTCCAAGGCCCCGCCGGCCCCCCCGGCGAGAAGGGCGAACCCGGCGAGGACG
    GCCCCGCGGTGAGGATTCTGGGGGTCTCCTCCCTCCGTGCACCCCCTGGCTGCGTGGTGCCGTTGTTCTT
    AGTCTGATTTCCCCCTCTGCCGCCCTGCAGGGTCCCGACGGCCCCCCCGGCCCTCAAGGCTTGGCAGGAC
    AGCGTGGTATTGTGGGTCTCCCAGGACAGCGTGGTGAGAGAGGCTTCCCCGGACTGCCGGGGCCATCGGT
    GAGTGGGTCGCTCTCATTTGGGTGCACTGAATCCTATGGGGTGCAGAGATGTGGGGGCCGCGATGCTCTG
    GAGCCCATCTCAGGGGTCGCCAGCCCTTTGGTGCAGCCCGGGGACACCGTTTGCAGGTGGGTTGGGGTTT
    TGCAGAGCTCCTTTTTCCCCACCAGGAGCCGCTGGTGCAAGGCTTAAAGCCGGGGCAGGAAAACCATCGG
    TGGTTATTTGTTGCAGAGGGGTCTGGGAGCCATAAAAAACGGGGAAGGGGCAGCGCTGGGGTCTCTCCCA
    CTCATGCACTTCTTTCCCCTCTTTCAGGGAGAACCTGGAAAGCAAGGAGCGCCTGGCTCTGCGGGTGACC
    GAGGTCCCCCCGGCCCCGTGGGGCCCCCTGGGCTGACAGGTCCTGCTGGAGAACCCGGGCGCGAGGTAAG
    CAAAACCCCACAGCATCACAGCGGCACCGGGCATCACCAACCCCATGGCACAGCTCAGCTCCCAGAGCTC
    CCCGGTGTCTTTTTCTCCAGCACTGAAAGGAGACTTTGCACAAATCCTGCTCCACCCGGGTTGTAACATC
    CCCTTTTCCTCCTAGGGCAACCCTGGTGCTGACGGTCCCCCAGGCAGGGATGGCGCAGCTGGCGTGAAGG
    TGAGCTTGCCATGCGCTCCCCATTGGCACTCGCCATCCCCGTGCCAAAAGCTGTGGGGTTTTGCACAGAT
    CTGACCTCTCTGTTGTCTGCTCGCAGGGTGATCGTGGTGAGACCGGCCCTGTGGGTGCCCCCGGTGCTCC
    TGGAGCCCCTGGCGCCCCCGGCCCTGTTGGTCCCACTGGAAAACAAGGAGACAGAGGCGAGACGGTGAGT
    GCTGGCACAAGGGTTTAGGGTTTAGGGTCTCCTTATGGCTGAAAATGTGCAGGGGTTCCCCTCGAGGTTT
    GTTCCTTGCACCAGTGCTGAGAGCATTTAAAGATGCTGTGAGGCACCAATAGCTGCTGATTGTCACTGTT
    GCCCGGATCTGGGGTGCGGAGCATGGGGCTGGCTCAGACACCCCCGAAATCCCAAATTCATGGCTTCGAG
    GTGGTGCTTCTGGTCGCTGGCACCTTCTGATGTCCTTTTTTTCTCCCTGCAGGGTGCACAAGGGCCCATG
    GGTCCCTCTGGTCCCGCTGGAGCTCGAGGAATGCCGGTGAGTGGTGCTGAGTGCATCGGCACATCCCACG
    TACAGAGCGTGGGGTCCTCCGTGCCAGGAGGGGGTCTGCCACCCTGAGCCCGACACAGCCCTGTCCCCAC
    TTTAGGGTCCCCAAGGACCTCGTGGTGACAAAGGTGAGACGGGAGAGGCTGGAGAGAGAGGGCTGAAGGG
    CCACCGTGGCTTCACCGGTCTGCAGGGTCTGCCCGGACCACCCGTAAGTTGGTTTGGGGAGCACTGAGCC
    CCCCCCCCCCCGTACGATGTGGCTCCTTTGGGGTCTCTGTGGCCACCGAGGCTCTGTCTGGCCCAAAGTG
    CTGACCGCAGAGCTGTGACCACCCCGGCTTCCTCCTCAGGGCCCGTCTGGAGACCAAGGTGCTGCCGGTC
    CCGCTGGTCCCTCCGGTCCCAGAGTAAGTCCTGACAGTGGTGTTTGGGGTGGTGGAAGGGGAAGGAGCAG
    CAGTGGCCTCCCTGGGCACCTGCAGCCTCTGTTCGCTCCTGTCTGCTCATCAGCACCATCGCCTTCCCTG
    CCCTGAGGCCCCGCAATGCCTTCACCTCCCCGTTTTGGGGCTCTCTCCTAGGGTCCCCCTGGTCCCGTCG
    GCCCCTCTGGCAAAGATGGCTCTAACGGCATGCCCGGCCCCATCGGTCCTCCCGGTCCCCGTGGACGGAG
    TGGTGAACCCGGCCCTGCGGTGAGTCCTGGTGAGGGGAGGCAGGGAATGGGGTCCAGCTCGCAGAGCAGC
    CCATCAGCATCACTTCTTTCTCCCATAGGGTCCTCCTGGAAACCCCGGTCCTCCCGGTCCTCCTGGCCCC
    CCCGGCACCGGCATCGACATGTCTGCTTTTGCTGGACTGGGTCAGACGGAGAAGGGCCCCGACCCCATCC
    GCTACATGAGGGCAGACGAGGCGGCCGGAGGGCTGCGGCAGCACGACGTGGAGGTGGATGCCACCCTCAA
    ATCCCTCAACAATCAGATTGAGAGCATCCGCAGCCCCGAGGGCTCCAAGAAGAACCCTGCCAGGACCTGC
    CGCGACATCAAACTCTGCCATCCCGAGTGGAAGAGCGGTAAGAGCTCCGCGTGCCTCTCCCGTCCTCCCC
    TCTTCCCCACAGGAGAGCATCCCCAGCATCCTCGCACCGACCTGCGGTCAGGTTGGATGTTAGGAAAGAT
    TCCTTGTCCAAAAGAGCTCTGGGCGCTGGGCTGGGCTGCCCGGGGAGGTGGGGCAGTCGCTGTCCCCATA
    GGTGTTGGGGAACTGTGGAGATGTGGCACTTGGGAGCGTGGCTTAGTGGGGATGAGGCAGCAGTTGGACC
    AATCTTCGAGGTCTTCTCCAGTCTTAATGGCTCTGTGCTTCTGTCGGTGTGCATGGTGGTGATGGGTGGC
    CATTTAGACTTGGCGATCTTTGAGGTCTTTTCCGATCTTAACGACTCCTAGACCTCCCCAACCCCATGAA
    CGCTGTTTGTCCTCCCCCCTGCAGGAGATTACTGGATTGACCCGAACCAGGGCTGCACCTTGGACGCCAT
    CAAAGTATTCTGCAACATGGAGACGGGCGAGACCTGCGTCTACCCGACCCCCAGCAGCATCCCCAGGAAG
    AACTGGTGGACCAGCAAGACGAAAGACAAGAAGCACGTCTGGTTTGCAGAGACCATCAACGGCGGTTTCC
    ACGTGGGTGTCCCCCGGGTGTCCTTGGAAGGATCGATCCCACCTGGGATGTCCTTCTTGCGGTCATGTGG
    ATGGGTTTTAATGAAGTTATAGAGGGTGATTCTGAAGGTGTAGGTTTGGGTCAGTTCAGCTCCACAAATC
    AAAGGGAAAGGATGGGATGGAGCAACTGAGCTCCCTCGGTTTGTTTGGCCCAGAAAAGGTGAGGATGAGG
    GGAGGCCTCACGGCCCTACAGCCCCTTACGGCCCTACAGCAGCGTTAGGAAAAAAGTTCTGCCCCGGAGC
    TGTGTTGGGCACAGAACAGCCCTGTGATGCCGGAGCTCGGGGAGCATTGGGACAACGCTCTCAGACATTG
    GGTTTGGGTCAGGTCCTGGGTAACGTGATGTGCAGGGGGCAACCAGCCCATGGGTGGGCTTTAAGGACCC
    TTCCAAGCCAACCATTCCATGGTTCTGTGATCTGTAAGGACCTTTCCAATCCAAACCACTCTGATTTTTT
    TCTCAGCCATTTGGGAACCTGAAGTACGGAAGTCCTCCCAAAAAGCTCCTGAGAGTAAGGTGGTCATAAT
    GCCCGCAGGCTTTAACTCCTCACCTCTTCCCTCCAGTTCAGCTACGGCGATGAGAACCTGTCCCCCAACA
    CCGCCAGCATCCAGATGACCTTCCTGCGCCTCCTGTCCACCGAGGGCTCCCAGAACGTCACCTACCACTG
    CAAGAACAGCATCGCCTACATGGACGAGGAGACGGGCAACCTGAAGAAAGCCATCCTCATCCAGGGATCC
    AACGACGTGGAGATCAGAGCCGAGGGCAACAGCAGGTTCACCTACAGCGTCTTGGAGGACGGCTGCACGG
    TAGGTTGCTGGGCGCCTGCAAAGGAAAGGTGCAGATGGGGAGGGGGATGCTGAGGCTGCGGGGATGAGGC
    CGGAGCAGCTGACAGCATCCCTGCCCTCCTTCCCTCCCCAGAAACACACTGGCAAATGGGGCAAGACGGT
    GATCGAGTACCGGTCGCAGAAGACCTCGCGCCTGCCCATTGTAGATATTGCACCTATGGACATTGGCGGA
    GCCGATCAGGAGTTTGGCGTGGATATTGGCCCAGTCTGCTTCTTGTAAAAAGGGTTGTTGTTATTTGTGT
    GTTTGTTTGTTGTTTGGTTGTTGTTTTTTGTTTCTTTTTTTTTTTTTTTTAAAAAAAAGAAAGGAATCCA
    GCCCAATCCCATAAAAGCAAACCAGTCCCACCCCCAGGACCCGCACGTTCCCAGCACAACTTCTGCACTG
    AACGGATGGCACGACCCCGCGCCCCTTCGGGACCCTCCGGCGCCGTCACCGGGCAGACTGCGAAATACAA
    CCACGGGCTTATATTTATTTATTGCCTTCCTGGAAGGCCTGGTTTCGTAGGGCGGGTGGAGGTGGGAATC
    AATCTGGCAGGTGTGACGGCCCCCCTCCCCACAAAGGGATCTGGCAAACGCAGGTATCGCGAATCCCCTC
    CCCTCCCCGTGTATCACCAGCAGGAGTGCTAATGTATCATACAACAGAAATGGTGCTATTCTTGTAAAAC
    AAGTCTGTATTTTTTAACATCAGTTGATATAAAAACAACAAAAAAAAAAACTTTTGGTGGAAAGTAA
  • Protein Sequence : Show Sequence
    >gi|45383309|ref|NP_989757.1| collagen alpha-1(II) chain precursor [Gallus gallus]
    MHGRRPPRSAALLLLLLLLTAAAAAQDRDLRQPGPKGQKGEPGDIKDVVGPRGPPGPQGPAGEQGQRGDR
    GEKGEKGAPGPRGRDGEPGTPGNPGPPGPPGPPGPPGLGGNFAAQMAGGFDEKAGGAQMGVMQGPMGPMG
    PRGPPGPTGAPGPQGFQGNPGEPGEPGAAGPMGPRGPPGPPGKPGDDGETGKPGKSGERGPPGPQGARGF
    PGTPGLPGVKGHRGYPGLDGAKGEAGAPGAKGESGSPGENGSPGPMGPRGLPGERGRPGPSGAAGARGND
    GLPGPAGPPGPVGPAGAPGFPGAPGSKGEAGPTGARGPEGAQGPRGESGTPGSPGPAGAPGNPGTDGIPG
    AKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGEPGIAGFKGEQGPKGETGPAGPQGAPGPAGE
    EGKRGARGEPGAAGPVGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPAGLAGPKGATGDPGRPGEPGLP
    GARGLTGRPGDAGPQGKVGPTGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLPGAPG
    LRGLPGKDGETGAAGPPGPAGPVGERGEQGAPGPSGFQGLPGPPGPPGESGKPGDQGVPGEAGAPGLVGP
    RGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGATGPAGPNGAQGPPGLQGMPGERGAAGIAGLKGDR
    GDVGEKGPEGAPGKDGARGLTGPIGPPGPAGPNGEKGESGPPGPSGAAGARGAPGERGEPGAPGPAGFAG
    PPGADGQPGAKGEQGEPGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGP
    PGPNGNPGPPGPPGSAGKDGPKGVRGDAGPPGRAGDPGLQGPAGPPGEKGEPGEDGPAGPDGPPGPQGLA
    GQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGSAGDRGPPGPVGPPGLTGPAGEPGREGNPGADGLPG
    RDGAAGVKGDRGETGPVGAPGAPGAPGAPGPVGPTGKQGDRGETGAQGPMGPSGPAGARGMPGPQGPRGD
    KGETGEAGERGLKGHRGFTGLQGLPGPPGPSGDQGAAGPAGPSGPRGPPGPVGPSGKDGSNGMPGPIGPP
    GPRGRSGEPGPAGPPGNPGPPGPPGPPGTGIDMSAFAGLGQTEKGPDPIRYMRADEAAGGLRQHDVEVDA
    TLKSLNNQIESIRSPEGSKKNPARTCRDIKLCHPEWKSGDYWIDPNQGCTLDAIKVFCNMETGETCVYPT
    PSSIPRKNWWTSKTKDKKHVWFAETINGGFHFSYGDENLSPNTASIQMTFLRLLSTEGSQNVTYHCKNSI
    AYMDEETGNLKKAILIQGSNDVEIRAEGNSRFTYSVLEDGCTKHTGKWGKTVIEYRLQKTSRLSIVDTAP
    MDIGGADQEFGVDIGPVCFL
  • Molecule Role : Protective antigen
  • Related Vaccine(s): Adjuvant arthritis DNA vaccine pcDNA-CCOL2A1 encoding type II collagen
3. Collagen type II
  • Gene Name : Collagen type II
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 12824
  • NCBI Protein GI : 169658375
  • Genbank Accession : AAHY01124867
  • Protein Accession : NP_001106987
  • Taxonomy ID : 10090
  • Chromosome No : 15
  • Gene Starting Position : 97975601
  • Gene Ending Position : 98004723
  • Gene Strand (Orientation) : -
  • Protein Name : transcript variant 2
  • Protein pI : 8.58
  • Protein Weight : 131740.08
  • Protein Length : 1419
  • Protein Note : Also known as Dmm; Lpk; Col2; Del1; Col2a; Col2a-1; M100856; Rgsc856
  • DNA Sequence : Show Sequence
    >gi|372099095:97975601-98004723 Mus musculus strain C57BL/6J chromosome 15, GRCm38.p1 C57BL/6J
    ATCCTTTCCAATGATTTTTTTTTTTTTGGTTTTTAATATCAATTGATGTTTTAAAAAATACAGAGGTGTT
    TGACACAGAATAGCACCATTGTGTAGGACACGCAGTCCCTGCGCCCAGCATCTGTAGGGGTCTTCTGGCT
    GGGCTGGGAACAGTCACTCGGGGGGCGTCTGACTCACACCAGATAGTTCCTGTCTCCGCCTGGACCCAGA
    GGTCTTACAGGAAGACAATAAATAAATAGAACACCGAGATTTTATTTTGCAGTCTGCCCAGTTCAGGTCT
    CTTAGAAAGGGGAGCACAGTCCGGGCTGTGAGGGGAGGACGGTTGGGTATCATCAGGTCAGGTCAGCCAT
    TCAGTGCAGATCCTGGAGTGACTGCGGTTGGAAAGTGTTTGGGTCCTTTGGGTTCGCAATGGATTGTGTT
    GTTTCAGGGTTCGGGGGTTTTACAAGAAGCAGACAGGCCCTATGTCCACACCAAATTCCTGTTCAGCCCC
    TCCAATGTCCATGGGTGCGATGTCAATAATGGGAAGGCGGGAGGTCTTCTGTGATCGGTACTCGATGACG
    GTCTTGCCCCACTTACCAGTGTGTTTCTAGGCAGAAAAGGGGAGGTGTCATTTGGGCGATGCCTTACAGC
    AAGGACTGGCTACTTAGCACCCCCAAACTAAGCAGCCACAAGGTAGGGACGACACTGGGTGGCTTGCTAG
    AACTTCTCTCCATTTGCCCAAGCCCTCTCCTCTTCAATTGTCCCCTTGATGGTAGAGCCCTCCTCTACTT
    GTCTTCCTCCTTCCCACAGAGGGCTTCCTGCCTGGGATAGCCTCTCCTCTCCTATGCTAGTTTCTACGAA
    TACTTCTATAAGAGTTAGTGAGCTTGCCAGCATCTTTATTGCCTCTGAATGAGGAAGTGGACAAGCCAGT
    ATGGTATACGCTCCTCCCCCATCCCTGCCTCTCAGCTTGTCTCCCACATGCCCCCACGACTCAAGCCTCT
    AATGAGTCCCTTCACTACAGGAAAGTGACATTTGTCACAGGCTGACCTCAGCCCCACTCACCGTGCAGCC
    ATCCTTCAGGGCAGTGTATGTGAACCTGCTGTTGCCCTCGGCCCTCATCTCTACATCATTGGAGCCCTGG
    ATGAGCAAGGCCTTCTTGAGGTTGCCAGCCGCTTCGTCCAGGTAGGCGATGCTGTTCTTACAGTGGTAGG
    TGATGTTCTGGGAGCCCTCAGTGGACAGTAGACGGAGGAAAGTCATCTGGACGTTAGCGGTGTTGGGAGC
    CAGGTTGCCATCGCCATAGCTGAACTGTGGGGCAGAGGGGCGAGGGTTGTGAGACCAGGGGCCTAGCATC
    TGCTGGGATCAGAGGCTAACAGTACATTCATCTGATAGGGGCTCAGAGCATCAGATGGGCCCTGGGGCAG
    GTGTGGAGCAAGCTCAGAGGACATCTTCTCTCGCTCTAATGGGGAAAATACTTTCTCCTCTTTCTTTCTT
    CTAAGGCCCAAAGGGACACCGGCGGAGGATGCCAAGAAGGAGCTAAAGAATCGGAGGAGAGAGAAGAGCA
    CTCAGGAGGGACCAGCTGAGCCTAGACCACCGGGCACTCACGTGGAAGCCACCGTTCATGGTCTCTCCAA
    ACCAGATGTGTTTCTTTTCCTTGCTCTTGCTGCTCCACCAGTTTTTCCGAGGGACAGTCGCTGGGTTGGG
    GTAGACGCAAGTCTCGCCGGTCTCCATGTTGCAGAAAACTTTCATGGCGTCCAAGGTGCAGCCCTGGTTG
    GGATCAATCCAGTAGTCTCCTGCAGGAAAAAGAGGCAGCATCCCATGATGCTTTGCAAGACAGCCTACTG
    GGAAACAAGTCAGGGGAGGCTTACATGTGGCCTAAGTGGGGGCCAGACAGAAGGGCATGGGAACAAAGAA
    ATCTGCATGGAAAACCTAATGCTAAAGAGGGGTCCAAGGAAAAAGGGAGAGGCAGAGCTTCCCTGCTGGA
    CAGAGTCACTTCTGTCATGGTCCTTCACCAGAACTTTCTAGGTCTAACCCCGCTTTGCACTACGGTACAA
    TGGGGTGTGTCTTCCTGGCTGCTGAAGGCGGACATATTGGTGGGCTGGCTCTCTCCTCTCTGGGGGAGGG
    GTCCTGCCCTCCAGGCTCACCGCTCTTCCACTCGGGGTGGCAGAGTTTCAGGTCTTGGCAAGTGCGAGCA
    GGGTTCTTGCGGGAGCCGTCGGGGCTGCGGATGCTCTCAATCTGGTTGTTCAGTGACTTGAGTGTAGCGT
    CCACCTCCACGTCGTGCTGTCTCAAGGTACTGTCTGCCTCGTCGGCCCGCATGTACTGCATGGGGTCGGG
    GCCCTTCTCTCTCTGCCCTAAGCCAGCAAAGGCTGACATGTCGATGCCAGGACCAGGAGGACCTGGAGGG
    CCAGGAGGACCGGGACTTCCAGGGGGACCCTGAAGCAGTAAATAGAGATGTCAGCTAGGAATCACAGGAC
    AAGGCCCTCCTCTGTGCCCATCTGCTGGGACTATGCTAGTCCTCTTCTTGCTCATCTGGTGGGGACAGAG
    GACAACAGGACCAAGAAAACCACAGCAACTTCCCCACTGCCTCCCTGGCTCTTCTGTTTCCCAGAACCCC
    TCATGAGCCCTGTGACCCTCAGCTGGCCCTCAGTCTGTAGGATGCCTTCCCCATTAGTTGCCAGCTGATG
    TTCCAAAGGCCCTATAAAATGCTGGGGATACCTCCGATGGTGGAAGGGGGAGGGTGGGGTGGGGTCAGGA
    CACTTACAACAGGGCCTGTTTCTCCTGAGCGTCCACGGGGACCTGGAGGCCCGATGGGGCCAGGGATTCC
    ATTAGAGCCATCTTTGCCAGAGGGACCAACAGGGCCAGGTGGACCCTATAAGCCAAGAGAGAGGAAATGG
    TAGGAAAATAGCCTCTGAGTAGTGTAGTGGAGCAGCTATGGCTAGGTGGGTGGCCAGCCCCATGGTGCTG
    GTCATACCATTCTGTCTCTGATCCCTGCATTCCTCAGCCACACTCCCTAAATGCAGCTCCATTTAGCCAA
    AGGACTAGCAGGTTAGTTCCATGTCCCTCCTAAGTCATGTTTATAAAAATCTTATTTTATCAGGCTTGTC
    TTCCAGCCAGTGTGACCTCAGATGTTTAGGCCACAGCCAGTGCAAGAGAGTCCCGCTTTTCTCGGGCTAC
    CTCCTGGAAGGACTGCTAAATCCAAGGTGCCCTGAAGAATCAATGCTCATATCCATCATCTCCTGGCTGA
    GGCCTTGGCCCACCCAGCAGAAAAGCTCATTCCACCCCCTGAAACCATCACACTTACTCTAGGGCCAGAA
    GGACCAGCAGGGCCAGAAGCACCCTGATCTCCAGAAGGACCCTGTGGAAAAGAAGAAAACAGCGGAGGTC
    AACACAGCTGGGTATAGAAATACATCTTCTTCAGAATGGGAAACAGTCACGTGCACCCCCCCCCCCTTGT
    ACCCACTAAGAGATTCCAGTGCCTTCTGAGAAAGATGCTAAGCCTTTTGGGTAGCAGGAGAAGCTCAGGG
    GGTCTGAAAAGTAGGAGGTCTTGGGCAGCCCCCTCATGGACAAATGACATGCAGTGACACCCACCGGAGG
    GCCAGGCAGACCCTGCAGTCCAGTGAAACCTCGGTGTCCCTTCAGTCCCCTCTCGCCCTGCTCTCCAGAT
    TCTCCTTTGTCACCTCGGGGGCCTTGAGGGCCCTATGAATGAGAAAGTCACAGATTTGGTCAAGTCTGGT
    CCACTATGTGTCTCATTTGAGACCTGGTGTCTCTTTTCCAAGGAGGACTCCACACAGGATTTTCCAATAC
    TTACTGCAATCCCACGGGCTCCAGCAGGTCCTGAGGGACCCATAGGACCTTGTGCACCCTGTTAAGGGGA
    GATCGAGAAAAGCGTCAGGGAATGAAGTGTCAGGACATGAAACCCCTCGTCTACATCTGCCTCAGGCCCA
    GGAGGCCCCAGACCCAGGGGCTACCTCTGGATACTCAAAACAGGAAAAGCAAACACGGAACACTCTCTAC
    CACTTGGTCACCCTCTTCACTCCTAAACTCAGGATACTCACAGCCTCTCCTCTGTCTCCTTGTTTGCCAG
    TTGGGCCAGCAGGACCAGGAGAGCCTGGGGGCCCAGGAGCTCCAGGGGCACCCAGTGCTCCAGTCTCACC
    ACGATCTCCCTGTAGGAGCAAAGTGTCAGATTCAGCAAAGAATGTTTCAGAAGAGGCAGGCAGAGATACT
    TTGTTTCTAGAGTGGCTCATTAGGCCCAGCACAGTTCCCGGAACCCCCATCCCAGACAGACGCCAGTCAC
    TTACCTTGACTCCAGCTGCACCATCTCTTCCAGGGGGTCCATCAGCACCAGGGCTGCCCTGGACAAGGGG
    ACATGAGGATTCACTTAGAGCCACTCTCTGTTTACTCTCCCACTCTGGTCTGAAGCATTGACCTGTGCAC
    TTCTGCCCAACTGAGACTTCGGGGCTAACACGTCCACCAAGCTTCCACCCACCCTCCTCTTTGTACACCC
    AGCCCTGGTCTCTCTGGGTGTCCTTCCCTCAAGATCATCCTGCCACACCAAGCCCTAGGGCTCACCTCTC
    GTCCAGGTTCACCTGCAGGCCCTGTCAGGCCAGGAGGCCCCACAGGACCAGGAGGACCTCTGTCTCCAGA
    CGCGCCAGGTGCACCCTGCTTGCCGGGCTCACCCTGAAAGGATGCAGACAAGGACCATGAACACACATGC
    TAAACATCCCTGCCAGGGCTCCACCCCACCCTCTTCAGCAGAATCAGCCAACAAAGGGATGCTCCCAAGG
    GTGCTGAGCTAAAGGAAGACCTAGGGTACCATTGCAGCAAGAACACCCAGTTAAGAGTTTTGAGCCTGAA
    AAGCATCCTTCACTCACCGATGGGCCGGGAAGGCCGGGGAATCCTCTCTCACCACGCTGACCAGGCAGAC
    CAACAATGCCCCTTTGACCAGCCAGCCCCTGGGGACCTGGAGGACCATCAAGACCCTAGGAGAAGAAGAA
    AGAGCTGACACAGGGCTTGGCCTGGCTGTGACTCTTCCCACCCATTGAGTCCATTCTGTGCGGCTCCCTT
    CCTACAATCCCCCACAGCCCTGCACCCTCCTCCTAGTGCTTAGAGGAGCTCAGGCGACACTCATAGGCCT
    AGCAGGGGACGTACAGAGGGACCATCATCTCCAGGTTCTCCTTTCTCGCCAGGAGCTCCTGCAGGACCTT
    GAAGACCGGGGTCACCAGCTCTGCCAGGGGGGCCACTGTCTCCTCGAACACCTTTGGGACCATCTTTTCC
    AGCAGGACCAGGGGGACCGGCGGGTCCAGGATTGCCCTAGGAGGAACAAATACAGAAAGTGGTAACTTGC
    CGTCCTCCTCATCCAAGTTACCGAGGCCACTGTGGCCTTGGGGGCCAAGGCATTCCACACGGTCCACCCC
    TGTGTAAAGCTCTGAGCAGCTAGCTGTGGCTCCATAGTCCTTGCTTAGCCTTTCTTCATCCACTTGGAGC
    TTTTCCTGCTGGATCAGCTTCATCCTCTATGACAGATACCTCCAGAAACAGCTCCAACCTTGACGCTCTG
    ACTTGAGTCTGCTGTTCACGCAGCAGCCCCTCCTCCACTCTCAGTGTTGATGGATGGAATTTTTCCTTAT
    CTCTTCTCACACTGAAGTCCCCCCCTCCGCCCGCCATTATCTGGTCTGGGGCTCATGGGCTTCTTTTACA
    GATTGTAGATCTAGATGCACTGAACAGGTTTAGGAAATATGTCCCAGGTGCAGGGCATCATGCCAAGTCC
    AAACCAGCCAGGAAGCCCCATTGCTCCCAGCTACCAATGGTCAATTTGGTAGGAATGGAGAAAAGTCAGG
    GCCATACGGGAGCTCTTTTGACCCCTATGTTTGGAGGTTTGCTCCAAATGCCTCTGACCCTATCTGGGTC
    TCCCACGCCAGAAGTAACCTGGAATGGTACTCACATTAGCACCTGGGGGTCCAACTCGGCCAGCAGCTCC
    AGGGAATCCGGTGGCTCCCTGTGGACACAGAGCGGTGGGTGAGAGAAAGGCCCTGGATACCTCGGGTTCT
    CAGCAGAGAGCAGAGTCAAGTTGGTAGGACTAGAGAGCTTGTTTCAGAGCTGGGGAATCAAGAAAAGTTG
    AGGACTACAAAGTAGCAAGTGAGTCACTTGAAAACAATGAACACTGCACCTGGCCACATAGTCAGGCATG
    GGGTCGGGGTGAGAGGTGTGGCTAGCCTTCAGGGAGCTGAGTGGCAGAGCTGAGGGCTGAGGACTCTAAA
    GTGACTGTGCGCACACAGCAGAGTTAGATGCCGCCTCTCCTGCCAGGCTGTAGTCATGTGGGCTTAGCCT
    GGGCATGTTAAAGGCTGGGCACAGAGAAGGTGGAGAATCCGGAACCTAACTAACCCACTGCAGAAATCAT
    GGCCCAACGCCTTTTTACATGAGATTTACTCACCGGGGGACCTTGGGCACCTCGGGCTCCCTTAGGACCA
    GTCACTCCAGTAGGACCCTGTGAAAGGAAGATGACAATGAGGTCTAGTGGACCTGAGGAGAGTGGTAAAC
    ATCTGTCTGAACCTTAGCGCAGGACCCCTGAGAACCGGGCTATCAGAGCACCGCTTCCCAACACTAGGAC
    CCCAGACAAAAGCTATCGTAGGTAGATCTGTGTGCATCACCACCGGAAGACTCTAGGGCTGGACTCTGTA
    AGCCTTCTACAAATACAACCCCAAAGTCTCCCCTCCTGGGAAACCCACTCTCTCTGCTGCCCCAGGATAC
    CAACTGCCTGTCCTGGCCATCTGAGGTCAGTGGACACAGCCTATACTCAGTGCTGAGGACCTAATACCCA
    ACCTGCGTGCCCCACGCACCATTGTCCCTGCACAGCCGAGGTGCCCACGAGGCTCCCAATTAGACTCTGC
    CTGCATGACCTCCCAGGGTCCTCCTCCTGTCCTGGCAGGCTCTGCCTCATGAGCTCAACAATACTCACCT
    GTGGCCCAGGGGCTCCCGAGGGGCCTTGTGGGCCGGGGGCACCAGCATCTCCTTTCTGTCCGGCTTCTCC
    TTGATCACCCTTGGCACCAGGCTGGCCATCAGCACCCTGTAATAGGAAGAAGAGACAGATGAAGGAGAGG
    CAAGCTGATCACCCCAGGTCCTGCCAAGAGGTTCTGGGAGTACATGTGTACGCGTCTACCCTGGGCCTGA
    AAACTGGGTTTAAGACTGGGCTGTTCTGTTGCCATAGAAATATGAAGAAAGTCCATTTCTCCCTTTACTG
    CCCTCTGGCGCCCCCTCCTCTCTCCCACCAGAAGGTGCCCTTGAGTTCTGGATGTGAAGTCAGACTGCAC
    ACATGGGTGGGAGTAAGGTAGACAGAGATACTCACAGGAGGGCCAGCGAATCCAGCAGGTCCAGGTGGCC
    CGGTCTCTCCGCGTTCACCCTGTAAGATGACCAGGAAGTGTCATGTCCGTCGCCTGCCTCTGTGCTCGCC
    TCAGACTGGGAACCCACTGCCCAGCCTCATGCTCAACCCCTCCCAGAGAGAAGGCGTCTGAGTGGCCTAG
    GTCCCGGGGAGTCAGAGCACTGGTCATGCCTGGGCCTGTGACCTCTGCCTTTCATGACTTCTCTCCTCCC
    ACCCCTAACCTGAAGGGCATGGCTGGTTTCCTCACTCCTGGGGCCAGGGAAAGCCTAATTTAGAGAGACA
    CACTCACCGGGGCACCTCGAGCTCCGGTACTTCCTGACGGGCCAGGAGGTCCGACTTCTCCCTAGGAGTT
    AAGAAGGTAGGGATTAGAGTGGGCCCGAGGACCCAAGTGTACCAGGAAGCCATGATGTTGGGAAGAGGAC
    TCTGGGTCCCTAGACCCCATTCCACATTAGGTTTCTTCCCAGTCCCAGCTGTCCCATCTATCTCTTCAAG
    GCCTTGGCCAATGGCACCCTTCTACCAGGCCAACTGCCACACAAGTCACACGTTAACTGGACCTCCACCC
    TATACTTCTACCACCACCATGGTAGGCAATGGCCAATGAGTGAAATTTGACTGTCTGGCAGGAGGTAAGG
    GGAAGACATTCTGGGGAGGTGGACAGAGGGATGAAGGTCAGACAGAGGGACAAAGGCCAGCTGCAACTCA
    CCTTCTCGCCGTTGGCCCCTGCTGGGCCTGGGGGTCCGATGGGCCCAGTCAGACCCTGGAGGGAGAAAGA
    TGGAGATAAAGGGAGAGAGTCAGGCCAGCATCATGGGGTTCCTTGGGGATTCTGGGAGCCCCATACCCAA
    TAGACTTTGGTGTACCCTCCCATGGTCTGAGAGCTGCCACTGATGTCTGGGCCCCAGCTCTAAAGGGAAA
    GCCCCTGTCGTCTTCCAGCCCCTCTTCTGAGTGTGAAAGGTCGTGGGAACACCTGTGTCTGTCTCCCAGC
    TCCTGAGTCCTATAAGCAACAGCTACTTACTCGGCCGCCATCCTTCCCAGGAGCTCCCTCTGGGCCTTTC
    TCGCCAACATCGCCCTGCAGAAGAGAATACCGGGTACCTTAGTGGGTACATCAGGAGCGACTGCCAGTTC
    ATCCCCGCCTGGCTAAGGTTCTGGTATCCTGTCCCTGCCTTAGGAAGTCTTTTCTGTGGGCTTTGGGCTG
    GTGACAAGGGGTAACGGGTGAGAAAACAGGGAGAAGGAGGTCATGATGGCAGAGCGGTTCGGTTGGTTGG
    TTGGTTGGTTGGTTGGTTGGTTGGTTGGTTGGTTTCGGGGTAGGGGTTCCTAGTTTCTCAAAGAATGGCC
    ATGATCCTCATCTTCAGAAGTACAAGGACAGAGTATGAAGCCTGGTGGTTGTGGCGTTGGGGAGAGACAC
    CCATCACTTACTCTGTCTCCCTTGGGCCCAGCAATGCCAGCGGCTCCTCTCTCACCAGGCATTCCCTGTA
    GACCTGGAGGCCCCTGAGCCCCAGGGGGGCCATCTGGGCCAGCTGCACCCTAGGATGACAGACAAGAGGA
    CTCTGTAGGGAACGGCATCTCTCTCTCTCTCTCTCTCTCTGCCCCAGTGTATACAGCCTGGGAAGCCAGG
    ATCCCAAACCCCTCTTCCCCCTTAAATCCCCTTCCCAGTGCCACCTCCCGCAAGTTCCTCCTAGCCACCA
    TCTTTCGTGCCCCCTCCCCTCCCTCTCACCCCTCAGTGAGCTTCCCTAGTGCTAGGTAGAAGACACCCGC
    GGGACTTGGCCTCACTTACTTTGGGACCATCAGTACCAGGAGTGCCAGGGAGGCCTCGGGGACCCTGAAG
    GCCCTGAGCACCAGGAGAGCCACGTTCACCTGGGAAACCTCGCTCGCCCTGTGGAAGAGACATCAAGCAG
    AAATCAGGCAAAAGTGATGGAGACGATGAGACAGGGGAGGGGCCCACAGGAAGAGATATGGGTCCCCAAG
    AACCCAAGGTGGGCTGCAGTGTTGGGAGACATGGGCCAAAATGAATGAAGGCAGGCCCAAGGATATACCA
    GTGCCGGGTAGCAGGACACTCACCCGAGGACCCACAAGGCCAGGAGCTCCAGCTTCACCAGGAATACCCT
    AGAGAATACAAAAAAGGATGTAAGAGTGAAGGCTGCCTGCTCCCTCTGGACTCAAGAGCGTGCCTCCCAT
    GGTGGCTGAGCATGTCCACAGGAGGACTGGGCTCAGACTTGTCCGTGGAGAGCAGGGAATCAGGTCTGGG
    GCATTCCCAGGCTCCACAGGGCTCTCCATTCACTCCATTTCCCTTGTCTCCTGTACATCAGCTAAACAAG
    GTCACAGAGCTCCCAACTCACCTGGTCACCTTGCTTTCCACCTTCACCTGGGGGACCGGGAGGGCCAGGA
    AGTCCCTGAAAGCCAGAGGGACATGGATTAGCAAAGAAGGGTGTCTGGTGCCTCAGCTTCCAGGAGGGCG
    CAGCACAAGGCAGACCCAAAGGAGAGAATTATTAAGGCCAGGCACGGAGAGCCACACCCTCTGATACCCC
    ATGAGAGGAGTGGGCTTGCCACCCTTAGTGCCTGCCCAGAGAGCTCTAGGCATAGGAGGGGATTGCATAG
    AGAGTCTGGTCCAGCCACCTACCTGGAACCCTGATGGTCCAGGAGCGCCCTGCTCGCCTCGTTCACCAGC
    AGGTCCCTGCAGAAGAAAGAAAAAGTGAGCCAAGCCAGGAATTCCAAGAACCTTAGGCCCCGGGGCGTTG
    ATTAGAAGCCTTCCTGCCCTGACCAACCAACACGTGGCAGGCCACTTGCTCTCCATCATCTACCCACACT
    TCCCTCCACTAGGCATGTCCTCTCATCTGGGGATAGTTCAGCCCAGATCCACATCTGTCACCTCTCCTAC
    TAAATGGATCCAAGGTCCCTCGTTTTAGCAGCAGGTACTCACACTGGGGCCGGGGGGTCCTGCGGCTCCC
    GTCTCACCGTCTTTTCCAGGAAGACCCTACAGGGCAGAGGAGGAGGAGTTGTAACGACTGATGTCTGTGG
    CGTCATCTGAGACCAGCAGATGTAGTTTTCCTTCGGTCAGGGGTTTTTAGATCAACAAGGTCTGCAACGG
    CTCTCCGCTTTCACCCTTCCCTGAACTTCCGTGGTGCGTCCACCATTTGCCTCAGTCTTTCTCCACATAG
    ACATCTTCCTCTGTACCTCAGCCTTGCCTCTGAAACCCACCAGCAGCCATAGACTGTCTGGGTGGATCCT
    GACACAATCTAACATGTGCTCATCCACAGAATCAACTCTTGAACGTCCTCCCCACCCCCCCGTTGCTTCT
    GTGAGGAATTCCACCAACACTAACACTGCACCAATAACAAGCCATAGGTAGTGGGGAGGACACTTACTCT
    CAGACCAGGAGCGCCAGCCAGACCCTTCTCACCAGCTTTGCCAGGCTCGCCCTGAAGGGAGGGACGAGAA
    GACCATGAGCTGGGTGATTTGGGATAGAGTGAAGTAGTAGGCTAGAGAGCTGAGGCCTGAAGCCTCAGTG
    TGGTGCACAAGAGAAGGTGCCATCGAACATCAGCCCCTTCTTTTATAGTCACTGGCCTCCGGGGCACAAG
    GCTCTATGGGTAATGTTGCAGTGCTGATATCTTAGGGTTTTAAGGTACCCAAGTTTACCCCAGGTCACAT
    GCATTCCTTGCAGGACATGGAGGACAGAGCTTAATGACATTGAGAGGCCACTGGTCACCCACCCTGGGTT
    AACTCTGCTCAGGACCCAGCTCCTTCTCCTCGAAGGATATAGGGAAAGACGAAGGAGATGAGAGAGCAAA
    CTGTCACTTACGTTGGCACCTTTGGGGCCAGGGAAACCCATGACGCCAGGCTGCCCACGAGCTCCCTGAG
    GACCAGGAGGTCCAGGGCGACCGTCTTCACCAGGGGCTCCCTGAAAGACAGGATTCATTCTCAGGACATA
    GTCCCTCTGGCTGTATCTCCCCATCACTATTTACTAAGTGTGTGCTGTGTGGCAGGCAGGGAACTAGGTA
    CCAGCCATACACATGCATCTAGGGTTGACCAACCCCACACACAGACCCAGCTACGGGTAGATGTTCCTGG
    CTCAGAAGTCTCTCCTGAACTTCTAATCTTTGTCATCACTCCTCCTCCTGGAAAATGTCCAAATGCTGTG
    TGTGTGTGTGTGTGTGTGTGTTTGTGATTTTCAGAGCTCAGAGTATAGGTCGCATCTGGCCATAAAACCG
    ACCATGTGTCTCTTACCCCTTCACAAAATGTACAAAGTCCTAGACTGCCCTCACCCAAACTCCCTTCTCT
    CTTCCCACCATCTACTGGACGATAATCCCTTATTTATCCTCCCAGGAACCTCACTCAGGCTAATAGACTT
    ACAGAAGGACCAACTTTGCCTTGAGGACCAGCGTCACCAGGGCGACCGGTAAGACCCTTGGTTAGGGAGA
    GAGAAGAGACCAAGGTTATTCGGGGTGGGGGAGGGGGACAGGCCCCAAATCTTCCAATTCAGGCAGCACA
    GCTTAGAGGATGCAGGTGGGAAGGGTGTGGGGGTCTGTGTAGTATCCACCTTACCCTGGCTCCAGGAAGA
    CCAGGTTCTCCAGGACGACCCGGGTCACCGTTGGCTCCCTTGGGACCAGTCAAGCCACTGGGCCCTCGCT
    CTCCAGGGGCACCCTATACCAGAGGAGACAGGAAGTCCATGGGATGCTCTGTAACTAGGGAAACAGGCAG
    GGACCAGGCCTCTCCTCCTCCACTCACCTTGGGACCTGCCAGACCATCTTGACCTGGGAATCCACGGTTG
    CCAGGAGCACCCTAGGGAGAAGGGACGAGGCAGGGGATGAGGATGCTTTTCATCAGCATGCCTGGAAGGA
    GTCCCACCAGGCTCCAGGGGTCTGTCCCCTCCACCTCCCACCACAGTGACTGTGGGGCGGTAGACACCGC
    CCGTGAGTCAGAGAAGCTAATGTCCGTCCAGGGCCTGCTCCCACCTCTCTGGGAAGAAGCTACTGTTTTA
    TGCCACCCTTGGGACAGAGGGTGCAGACAGCATGAGGATGCTGTGACCCTGGTGTTGGATGGGGAAGAGA
    AGAGCCACGTTATCCAGAAAAGTCATTGGGTGTGGAAGAAAGGATGAGCCACGGAGCAGCGGGCCTGTGA
    GCTGGAGCTCCTTGCCTACTTACTCTCTCTCCAGGGGGTCCGATTGGTCCAGCACCACCCGGCTCTCCTC
    GAGCACCTCGTTTGCCTTCTTCACCAGCGGGGCCAGGGGCTCCTTGGGGCCCAGCAGGTCCCTGAAGACA
    AGCACAAGAGGGGTGGGATGAGGTTTCCACCTAGCCTTGGGGAAAGCACTGGCCTGGTGTGCAGAGGAAG
    GGGCCTTGGAAATGTTTCGGGGCTTGGCCAAGGGGGTTAAAGGGTCAGCCATTGGGTCTGGGGGCCAGAA
    ACAGCCCAAGAGGAAGAGATGGCTTGGAACTCAAGCTATTCGAACCAGGAGAGGAGGCTGTGTCAGGGTC
    TTCTCTACCCTACTTCACTCCCTTCTCCAGGGACAGCAGGAGAGAGCCCGGGATGGGAAGTGGCTGTGGG
    GAGGGGGCACAGCGGGGGCAGAAGGGTGACCTGTGGGCAGAGCTACAGCAAAGAAAACAAAGTCTTGAGG
    GCAGATACTCACAGTCTCTCCCTTGGGGCCTTGATCACCTTTAAAGCCAGCAATGCCAGGTTCACCCTAC
    GTGAAAGAAAGAAATATGCTCGCACTGGCTTCTCTCCATAGAGTCTGCTCACCCTCCTCCCAGAAGCCTC
    CTACGGTGAGCAGGAGGGCACAGAAAAGAGGAGCCTGTAGGTAGGACCTGCCAATCTTTTTGGGCTCTTA
    CCGCCTGACCTTTGGGGCCAAGGGGACCAGTTGCACCTTGAGGACCGGGAGGGCCACGGGGCCCAGGGAA
    GCCAGGGGCACCAGCAATTCCAGGAGCACCCTGTCAGCATGAACAGAAGAGAGGCACGTGAGGAGGATGA
    GGAGGGACTAAGCCACCTTGGGAACACAGCCCAGGGACAATACTTACAGCGGATCCTTTGGCTCCAGGAA
    TACCATCAGTCCCTGGGTTACCCTGACAAGAAGATGGTATAGCGATAAAATAAACAGCAAAGAAACAACC
    CAACCATTCGGCCCATTGGGCCCCAGTCTAGGGGTCAGGGACAAGTGCTGTCTGCTTCAGGGGCATCCCT
    CCTGCCTGTCTCATGGGGTGGACTACGGGTGAAGGGAGACAGTCGTGGATGTCAGGGGGAGGTGTTTGGC
    CAGCATGACCATTTAGTGGTCCCAGTGACTGGGACTCCAGGTGACATGTACATTTGTGACAAGGCCACAC
    ATGCCATGCCTTCCAGGCCAAAGAGATGAACTTACAGAAGCACCTGCAGGCCCAGGGGACCCAGGATTGC
    CAGGCTCGCCACGAGAACCTTGAGCACCTTCAGGACCGCGAGCACCAGTGGGACCAGCTTCGCCCTGGGA
    AGAGAGTGGGGGGGCTGAAGTGGGGGTGGTGGTGGCAGCACTTCACGGCCCAGTGTGCTTGACTCTTGTG
    TGTGCTCACACTCCTGTGAGTGAGTTGCGTATGCATGGGGGTCTGTCTTTTCTGTTCCCACCTGACCAAG
    CAGCATGAAGAGCCGATATCCCACCCCTGCCTCTTCTACCCTCACCCCTGCCCTGTGACCCCACACACAG
    CTGCACAGATAGATGCCTACTGCATTCCAGCAGCAAGCAGCTTGCTGGTCCTTCAGTCCAGCAGAAAAGC
    CTGGGTTAGGAGACCTGCCATCTTAGGGCCATGGGATGATGGACAGATGGGCCTCCACTCCCCCTTCCCC
    TCCTTTTGTTTTCTCTTTGACAGAGGAACAGAAGCCAGAAGGGTGACTCTGTCTCCTCCAGAGCCTCAGC
    TCAGAAGTGGCTTTCTTTATGGCTGCTGGGTGATCAGAGCTGCTCCAGGCTTGAGCCTCAGCCTGTTTGA
    AGTGCCAGGAAGCCAGGGCTGAAAGCAAGCTGAGACAGGCTCTACTTTTCTTTAAGTTAACTGGAAGCCC
    CTTATCCCCCATGTAAAAATTCTTTTTCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT
    CTCTCTCTCTCTCTCTCTTCCTCTCTTATTTTTCTTCTTTCTTTTTCCTAAAAGTCACAGCTCTGTGATC
    TGGCTCTCTGGACTGGAGCCTCCTCCGGGGACGGCTGCTGCCCTGGAGAGAGGTCAGAGCCTCCTCAGGT
    TTCTCCACACTGGCATTCTTACTTCACTTTGGTAATTATTATTTTCTAAAGGTCAGGCCCCCCTCACCTT
    CCGCTGTCCCTCTTCACAATGGGTCTCCTTCACAGGGCTCCCAGGGGCCTCAGACACTGTCTGGTTGCCA
    TGGAAACCCCCAGGCGTAGAAAAGCAACCTGATGCCAGTGACACAGGGAGGCTCCTTACTCACTCCCAGG
    GACGTTCACACTGGAATCCCTCCCGGGCCACAGGGGCTTTTCCTTGAGCCGGCTGGCCTCCCGCTGGTCT
    CTATTAATCCCTGGAAGGACCAGGTTAACAACAGCCCAGCACCCCAAGTGAAGTAGAAATAAGAAAGGGG
    CAGGGGATGCACAGAAGAGTCGCCTCGCCTCTGCCCCTCCCCCTCTATCCCTTTAAGCTCCTTCTGGGTA
    CCTCAGCTTTTGCCCACCCCCAGGCTTGTTTTCTTCTTCCTCCCCTCCCTTAGTCTTCTTCTCTCTCTCT
    CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGGGTCTGGGGGGGGAGG
    GTTCCCATCTTTGACTACTAGGTGGACTTCAGCCTCTACAACCGAACCAAACTCTGTCAGAGAGAGATGC
    TGGAAACACACAGAGAGGCTAGGGGGGATGTGTGCCAACAGGAGCCTTCCTTAGCAAGGACCACAGGAAG
    ACTGGGTGTCCTGACCCTCTGAGATTCTCAGCTTGACCAGCAGATCACTCACCTTGGCACCAGGAGCACC
    AGGGAAGCCAGGACCACCTGCGGGACCCACAGGACCCTGGGGAGAAGAAGGGGACAGAGGGTGAGCGCTG
    GGTGTCAGTGAACTCTGTCCAGGCCTCAAAGAGAGACGCCCCACCTCCCACTCCTTCCACCTCTGTGGCT
    AGAAGAAACTGCCAGGGCTGCTCTCCCCAAAGACCTCCAGAGAGACACCCTGCTCCTCTTGGAGAGTTTC
    TGAGTCTGTGAGAAGCCACGGGGCTTTGACCTCTCTCCTTAAAAGAGGAGACTGCTAGGGGCAGCAGGGG
    ATGTCAGGCTGCAGTGTCAACAGCATGCTCACGGGATGAGCCACCTCCTTCCTGCCACAAAGTACAGGGG
    CAACCCCACAGACTTCAGGGCTGCACAGCTACTGTCACCTGCTTAAGAAGGACAGCAACTTACCGGAGGT
    CCAGCGGGGCCTGGCTGGCCATCGTTACCCCGAGCACCCTGTGAACAGCAATAGCACAAAGGAGCCAATT
    AACACAGTGTTGGGGTGTCCCAGGCTGAGCATGAACAAAGCAGAGGAACCTTCCTGGGATATCTGTACAA
    GGGGGCAGAGAGACAGACAGAGAGAGGGCTACAGGGCACTGGCCCTCGTGGGTAAGGAAGCTTGGGCAAC
    TTCCCAGCAGACCTGTCACTTCTAAAACTTCAGTTAGGTCCCCAGTATTCCCGACTTGAAGCTGAATGAT
    CATTCAAAGAATCCTGTGGGCTGGGCCAAAGGAACCGGACAGAAGGAAGGAGGAGCCTGCCCCTGTGCTG
    GGTATCCTGGCTTAACAGAAGCAAGTCTTCTCTACCCTCTGGTCCCTCCCAGTCAGCTGCTCTTCAGGAC
    AGCACAAATACCCCCACAATGTTCCCTGCTGACCGGGAACCTGACCCGCGATGAAGACAGACCCTGTCTA
    CAACCGCGCCTGAGCTCCTATCTCAGAGACCCTTGGCTTTGCAGGGGTGGGGGTGGGGGGTGGGGGTGGG
    GTCGAGGTTGTCCCTTCTCTAAAAGACTCGAGCTCCGAGAGTTCCACTGGTTTGGGGTGAAAACATCTTT
    CTATTTTTCTCCAGTGCCCTTGAGAATTTGTACTCTAACTCCAAGAACCGCTATGTTAGGCACATCCAGG
    TCTAGGACTGGGTCCCTCCCAGACCCAAAAGGCTAAGCCAGTGGCTCTCAGCTTTCCCAACACTGTGACC
    CTTCAATACAATTCCTCACGTTGTGGCAAGCCACAATCATAAAATTATTTTCATCGCTCCTTCACGACTG
    TAATTTGGCCACTGTTATGAATCATAATGTAAGTCTTTTTGGAGATAGCGGTCAGCCAGGGGAGTCACAA
    CCCACGGGGCTAAGGGGGGACTTTCTGCACGTCTCTGACTCTCAGAAGCACAAGGCAAGGATGCTCTGCG
    GTGCTCAGTCTCCTTCCTGGTGTGAGCCATCTCTCTTCCCAGAGCCTCTAGCCTGGCAGCCAGAGGGAGG
    GGCATGGCGAGACAGCAAGACTCAATAGAAGGAAGAGAGAAATCAGAGCCTGATTTCCTGACCCAAGCGC
    CCTGGAAAACTCTGTTACTGGGGAACCCTACAGTACCTTCCCTCCAGGAGTCAGCAGAGAGCAAGGCAGG
    GGGAGAGGGCCCAGTCTGAGCTGCCTGTGTCTGAGGCCTACATGGACAACTTCTAGGCTCAGAAGGCCTT
    TGGGAGTCCTCGCAGCAAACAGCAAGGCAGAGTGGGGAGCCCCGCCCCGCTCCCACCAAAAGGCCCAACA
    CACATGTAGAGAACATTCTAAACAGAATCTCCTAACAGCGCTGCCACTGCTTTGTGGACGAGACCACCAA
    GGAAAACCAAGAACTGGGGCTTGTGTCTGGGAAGAAGGAGAGACAATGCGAGAGGCAGTGAGTGAGCCAG
    TCTGGGTTTGAGCCTCGTTAAAATCCCCAGGCCAGGGTCTCCCGACTCTGCAGAAGCAGCAGGCCCAGAG
    CTTCCTTCCTTTCATACAAAGCGCCCCTGGGGCGAGGCCTTTGGCCAGCTCTGGTTGGCAGTGCGAGTGC
    AGGCTGCTGGTTTCACAGACCTGCTCCTGAGGGAACTTTCCCGCCCAGATGCACGGCCTGGAAAGCAGCT
    GCTTACAGTTGCCCTGCAAGGACCTAGGAGGAGGGGCCTCTGAGCTTGGTTGGCTGGGTGTAGACCAATA
    GAAAGCCGTAAAACTTGGTTGGCTGGGTTGCAGACCAATAGAAAGCCGGAATCTTTCTTAAGCGCGCCGT
    TCATCACATCACTCGCCTGACCAAGCACCTACAAGGCTTCTCTATTGCTTGGGGAGCCAAGATAACGGCC
    TCTCGCTGGCACCCATGCCCTCCACTGCCTGCCAGTCCTTTAGCTCATGGTTCTGGGCCCTCGGTACACA
    GTCTGTCTTAAGCTAGTTATTCTTCCCCGGGGACTCAGTTCCCTCTTCTCTAAAATGGGAACGAGAGATT
    CAAGTTCACAGAATGAGGGGGAAGATTAGACAAAGAGAAGCAAGCCAGGTTCTAAATCAAGGGTCACGAG
    GTGGATGCCGCTGGTGTCCTATCTCGACACTACTCAGAACCGCATCAAGGGCGCGGAATCGAGAGAGAGG
    GCAGAGTGTATAGCATAGAGGGCTGGCACCATCTAAGCCTGATCCTGAACTCTAACATGCACGGCTCAGG
    AAGGAGCAGAGGCTATTGAAGGGGTGACCTGAAGGATCCCGACTCCGGAAGAGGACTCTCACCAGCATAC
    TCTCCTATAGTACGCCCTCCTTCCACCCTGCAGGCCCCACCCCACCTGAAGATTGGGCGTAGGAAACCTC
    AGGATAGAAAGTTCTGGTATGATGATGCGCCCTAATCCTCACCAAGTTGGATTTTGTTGTTTATTAGGTA
    TTGGGGGGTGGAGTGTGGAGAAAGGTCTTATGTGTGGCGCAGGCTGGCCTTGAACTTATGATTCAGGAAC
    TCAGCTTCTTGGGTACTATGATTATAGATAGGTACGTCCCTCCTGGCTGGGGTTACCACTGAGTTGATTT
    GAGGGTCAAGAGAAATTTACTTGTAAGCTGTAAAGTGTTGAGCGTGTGGTGGCACCGGGCTTTGGGGGTT
    ACTCACAGCAGCACCAGCAGGGCCAGTCCGTCCTCTCTCACCAGGCAGGCCACGGGGACCCTGCAACACA
    CACCAGCGCGGTTTAAGTGTGAGTTGGCATAATGATGGTCTGCGTCATGACTTCTTCCCACCCACGGTGG
    CCCCATCTCGACCCTAGATCCTGCTCCCAGGACACAGGGGTTCCTTCAGGGAGACCCCTGTGTCTTCCTG
    CCCCTTCCTATGGAGAATGCAGCTCGGTTCTCAAAACAGATGAGTCACCCAAATCATTGAAGACAAACAG
    ATTTTTTTTTAAGAGTAACTGAAATGTGCTTTAAACGTGCCTAACTGTTCCTGTTTCGAGGAAGGGCGTG
    TGAAATGTTGAACAATCTACGCTGCAGGTTGGGTTGGCTCATAATCGGATAAATCTGTGCGAACAGCTTT
    GCATGAGTGCATGAAGGAGGAAATGCTGCTGTTTGCAGGGTAGCTGTCTGACAGGTGTTTCGGGAGTCTT
    AAGAGTGTGGGGTGGCTTCCCCAGGGGTGACTCTCATACTCACCATTGGGCCCGGGGATCCGTTCTCACC
    AGGGGAACCACTCTCACCCTGGAGAAAGAGACATATAGGGTCAGTGTCTGGAGTCTTGGCTTCCAGAAAC
    TTCCTAGGTAAGTCATCGATAGGTCTGGCTGGCTCAATTAGGCCTGAGACATGTCAATTTCAGTCCGGGG
    AGAGACGATGTCCCCAAACTCTAAGGCCTGCTGCTCATGTACCAAGATGTAGACTGGGTGTTCCCAGCTA
    TGTTCTAACTCCCGACCCCATCAGCGCCGCCTTGACTGGACAGTTGTCCCCTACCGCCACCCCCTGTGTG
    TCTTGTGGCCCCTTTACCTTCACACCCGGAGCACCAGCTTCCCCCTTAGCACCGTCGAGGCCTGGGTAAC
    CCTGGGAAACAGGACGAGGGTTCCGTCACATGTCTGGGAGCCCACCCAGGAAATAGTGTGCATAACCCCC
    AAACATCCTATCCCATGATACTTACTCTGTGACCCTTGACACCGGGGAGACCCGGGGTTCCTGGGAATCC
    ACGAGCACCCTGCAATACCAAGAGGAGGTACTCAGAGGAAAGCAGAGATTGGGTGAAGGCTGCTGGATAG
    ACTGCAAGTCCAGCTCCCTCAGGCCTACAGAAACAGGCATCCAGGTGTGTCCACAGCTCCTTCAGCCCAA
    CCCAGAGCATCACCTTGTGATCATACAAACACGGTATCCAGCGTGAAGGCATTACGTACACAGTACCGTT
    AGTACCGTGAGCTAACCAAGACGAGTGAGCCATATGGACTCAGAGGGAGACTGGAAAAGCCTGTGAGAGC
    GAGGAAGATGAAGCTTTACCTGAGGGCCAGGGAGGCCTCTTTCCCCAGACTTTCCGGGCTTCCCAGCTTC
    ACCCTGAACAGAAAGAGAGGCATCTCATTTTTCTTGGCAGTCTCTTGAGCGTATTCACTCCACGGCTGGG
    CATCTGCCTATCTGCCCACCTTGACCACGCCCTTCCTGCAGATGCCCGTGACCCAGAATGCCTCTGTGGT
    TTATCCCTTGGCAAGTTCCTGGGCCCTCACTCCACTGTCCCCAGCAAGCGTCTGGTCCCCTCTCCAGAGC
    CCTGTGGACTGGCCTGTATGCCCTGATCTCCAGAGGCCTTGCTGTGTGCTGCCAGTCATAAAAAAAAGCT
    CAAGAAAGCCAGCTCTTCTCTCTATTTTTTTATGTCCCCCTCCTCTGTAGACATTCTGGCTCAGGCTGAG
    TTTGTCTATCTCATTTACAATTGAGATGATGGTAGCCTGTTTGTTGACCGACTCTTGCTTTGTTTTTCTA
    CTCTATACCGCTGTGCTTTGAGTCCTCCCATCCCGGCTGGCTCTCTCCTTTCTCATTTCTCTTCCCGAAG
    TTAATGATGTTTTAATTACTCTCCTTCTTGTGGGATCTAAAGGGATCCCCAGGGATCTGGCAAGCTGAGA
    TGCAAATAAAGGATATGTGGGTGGTAGATAGCTGGAATTTAGGACTGGCTAGCCTGGTTCCTCAGGACGT
    CTCTCTACCCCAAGCCCCAGCACATCCTCTCTCTGGGTCTGGGGCTTCTTGGGTCTACTCACGTCGTCAC
    CAGGTTTTCCAGCAGGGCCAGGAGGACCTCGGGGACCCATGGGACCCTACAAATAGAGAATTCAAGACCT
    GGTTGGAAGGGAAACACAACTCGAGGCGGTCGCTTTGGAAGCTAGTTACTCTACAAGGAAGAGAGACAAA
    AAGGTAGTAACAAAACTGTAAAGCAGATCATGGTGGGAGGAGGGGACTGCGCAGGCTGGCGACCCCTAAG
    GGGAAGTGTCCCGATGGAGTTCCTGCCATATTGATTCTCTGCCGTTGTGCCCCGTGGGTAAGGCACTCCC
    ATTGTGGGAAAGGGCTGGCAGAGCCTGGGAGCAGACGGTAGCTTGGGTAGAAGATTGTCCCCAGAGCCTC
    CTGGGAGAGGGTAGCCTGTGGTACTCACAGAGACACCAGGCTCGCCAGGTTCACCAGGATTGCCTTGAAA
    TCCTTGAGGGCCCTAAAAGGGGAAATAAGGACGCCATGTTGACCTCCAAGCTGTGCCGCCGTCATGTGGA
    TGATGAGCATCTACAGCGCCCCCAAGAGGAAAAGACTGAACACTTACGGGGGCACCGGCAGGGCCTGGGG
    GTCCACGGGGTCCCATGGGGCCCTGCATTGGAACAGAAAAAACGAGACGCTTACTGGCCCGCTTTATCAT
    TGGCTTCTAGGATTCCATCTTAGAAGAGGGACAAGAAGATAGTAAGGGAGCAGGAGGGGGAGGGGCAAAG
    GGACTCCAGCGGCTGCTCCCTCTTCTTTCCCACTCCCCACTCAACCATCTATCTTTATTTATAGGCCCCA
    TTTGGACCGAGACGCTGGCCTTGCTCCCTCCTGGATGGCGGCCATGTTTACTAAAGTCTGAATTTCATTT
    AGCATGTGCCAAGCCATCGCTGCAGGGTGAGATTTCCTGCTGGAAGCCTGGTGGATGCTGAAGAACAGTC
    GCTGCTGTCTGCATTCTTCAGTTCTCCTTCCAAGAGCAGTGGCAACCAGCCCCCTAATACATCGGCTCTG
    TGCATCACTCCGTCCCCCTTCCTCCTAGACACACTAGAGCGTCATCCAGACTGGCATCCATCACCATTAA
    AAACCAAACTCCCCAGGGTTAAATGAGAGGTTTCCCATAGCTGATGGAAATGAGATGCCCTAAATCATCG
    GCGAAGGCAGAGGTTGCTGGGTCTCTGGCTTTGCGAAAGGTGCCCTCCTCCCGTCCGGTCAGCACAGAAC
    TGGAATCCTACCATGGGCCCTTGCATGACTCCCATCTGGGCACCACCAGCCTTCTCGTCATACCCTCCAG
    CCATCTGAGCCGCGAAGTTCTGCAAAGCAACAGAGCCGTGTTAGGGGGCTGAGAGGCCCTGGTGTCATTC
    CTAATTGCTCGGAGTTCTACCTAGTCTCTGGTTATCAGGGACTAGCCGAGCAGCGTTGGTCAGAATTGCC
    AAAGGGATCTGACAACGTTTTGGGAGTGTGCCCTTGCGACAGTGGTAGAATCACTTCCTTGGAAAGTCAA
    GTGTTATTTGTGGTGGTCCCGGTCCCTCACGCTCTGCCTTATAAACGTTCATATTCTGAAACATTCATAA
    GTAAAACATCGCCTTTAAAAGGCATCCTTTGTTCTCTCTGATGTTGATGTCTGGCAAAGGGCTGAAATCT
    GGTCTTGAGGTATTTCGGGAAGGGTTATAGTAGGCGTCTGTGAACCCGCTTAAGTTGAAGGATTTAAAAC
    ACAGCAGACCTCCAAATGGCACACCTACAAAGTGGGCAGTCATATAATTTGTTGACTGGTGTGATTCCAA
    ACACGACAGTAGCCGCACACCTTCACTCAGTGGGGCTCTTTCATAAGAATGAATAGTTGCTTGCAATGTA
    CTTGGCAAGGGCTATGGACAAATGTCTTCCAAGGACAGTGGTCACTGCTCTTCCAGAGAAATACAAGTGC
    CCAGTCATTCTTTGCGGGCACCCCTACACCCTCCCCCAAGAATTAACCGCATGGTGGGGTGCCAGGAAGC
    CAGATAGCTGAAGGCCTTCTTTACAGAGACCCAGTGAGTGAAAGAAAGTCAGCCCTTAGCCCCCAGCGCC
    TCGAGCCTCATACACAGATCTTCCTTAATTACAGGCCGTGAACAGGCTGTGTGCGTACTGCTGGCTGGCT
    CTGGGAGGCTGCCAGGTAAGAGAGTAGCAATTTAGAAGCCACAGTTCTGGAGGGTCAGCCTGGAGAAAGG
    AGAACTAAGGGCACTTACTCCTGCACTAAGGCCAGGGGGACCAGGGGGACCTGGAGGGCCAGCGGGGCCA
    GGATTTCCAGGGGTACCAGGTTCTCCATCTCTGCCACGGGGTCCAGGCGCACCCTTGACAGGAAGAGAAA
    AAAAAGTTATCAGTGGAGCACCGCTACCTTTGGAACCATCTGCCCTGGGCAGCACTTGGGAGGTAGCCAG
    GCCTTTAGACGACCCAGGGGACACAGACATCAGTATTTGTTGCTGTCTGCTCACCTTTTCTCCCTTGTCA
    CCACGATCACCTCTGGGTCCTTGTTCACCTGCAGGTCCCTGAAGGTGAGGGGCAAGGTGTGTTGACATGA
    AACTAGAGGTCATGATGGGTCAGGGGCAGTCAGAGAAGAATGGAAAAAGAGATCTCTCCCTTTCTTACCT
    GAGGGCCAGGAGGTCCTCTGGGTCCTATGATCTGTGTCAGAGAGAGCCATGAGCCCATTGGTTAGAGGGG
    TGCATTTGGGTCAGCAGGTGCACGGGTTGTGGCGGAGGTGAGTCGGGTGGGGACGGGTCACGGTGGGGAT
    AATTTGTACTTACATCTCTGATATCTCCAGGTTCTCCTTTCTGCCCCTGCATCACAAAACATCGACAGGT
    GAAGGCTGAGCAAGCCCGCTGAGCCCCCACACAGAACCCCACCCCACAACCCTAGCCTCTCAGATGGACA
    TCTGAGGGCTAAAGAAGAGGCACAGACCAGATTAGTATCTCAGTACCCCTGAAGAAGGACACGACACATG
    AAAGCAAAACATGGACCAGTGAAATGGCCCGGTGGCTAAGGCTCTGACAGAGCCGCCCTAATGCCCTATG
    GTGGGAAGAGAGACCCAAGTCTTCAATGTTATCCTCGGGCCGCCACACATGCGCCATAGCACGCTTGTGT
    CCACGCTCATACATACAATCGCGTGGGCAGGGAGTGAGAATGTAATTAGATTAATTAAATTAAATTAAAA
    ATGGGGTGCCTTACCTTTGGCCCTAATTTTCCTGCAAGGGAAGACAGAGAAGGAGAAGTGAGACAGGGAT
    GATAATTTGACACTCAAACTCTCTTCGAAGGATCAGAAAGGAAAGGGCCACAACCGAGGAAGGAAGCGCA
    TCCCTCTGTCCCCGGAATCCCTCTGTGGTGGGGTTAGGATCACGGAGCCCTTTTGTGGCTGGGGACTTAG
    AAGGGACAGAGGTGCCTCTACCTCATTAGTCAAGAAGCATGCATAGTGTACAGGAATATGGCGGAGAGCA
    GGGGAGGAGGCAACCAGGGGTTGGAGGGCAAAGCGCAGAAGACCCAGAGGTCAGGCAGTCCTTCCCAACC
    CGTGCCCTCCCAAAGAAGAGTCTAAGGAAGCCAGCCTTACATTAGCGTGGAGTCTCCTTGACGTAAGCAC
    AAAAGAGAGCGGGACACAGGTGTTACAAAGAGCATTGCAGCCAGGGCCATCCCAGACCCATACGCCCCTC
    CCCCACCACAGCAGCAAGCTCCACTGGGTCCTCACACCGCACTGGTATGGGCGCCTCCCTCTGAGAATCT
    GCGCCATACCAGGCAACAGTGTGGGGGAAGCATGCAGGAAAGGCGACTTCTCGATTCCAGTTCAGCCCTC
    TTCTCCATAGGAGTAGGAAGGGAACCATGAGCCTGTGCGGGCATCCCCCACGCTCTCCGTAGGGAGATGG
    GACACTTGCGTGGGTGCTGGGAAGGGGTCAGCTGTACTCTTCTTTGTTAGCACTGGTTCTAGCTGGGAGT
    GGGTAACCACAGCAGCTTGCAGGGGCACGATCATCCTTTCAACTCCCAGAGCCCAGCTACCCCTTTGGCT
    TTTCTTAAATTAATTAGCCGACTTGACTCAGGAGGGAAAACTTTTGTGGAAGCATAATTGTGTTTCTGTA
    CGCATCCTCCTGGGGTGTTAGGTGAGGGAGGGTCTCTGTAGTGATTCAGGCTCACCAGCCTTCAACCTGG
    TGGAACCGAGAAGTGGCCTTGCCTTTGTGCCCCTGCTGGTGGGATCAAATAGATTGATTAGCAGCTTCTC
    CGCTTCAAAGAGAAGGCCAAAACCCACGGAAGGCATTTGCTGTGTCTCCGAGTAATTTATTTATGTTGAA
    TAATAAATAAATAAATTACGACCACTGGCAGTGGCGAGGTCAGCTGGGCAGATGGGACAGCACTCTCCGA
    AGGGGATCTCGGGGTTGAGGCAGTCTGGGTCTTCACAGATAATGTCATCGCAGAGGACATTCCCAGTGTC
    ACACACACAGATGCGGCAAGATGAGGGCTTCCATACATCCTTATCTTTATACCTCTGCCCATTCTGCAGA
    CAGCTGCCAGCCTCCTCTGCACCAAGTGGGGTGGGAGGGGCGGCGTGCCAAGCAGGAGCGGGGAAGAGCC
    AGAGAGAAAGAGAAAGAGGTTGTAATAAACTTGACATAATTAGTCCAAATGCTAGGGAATGGTGGGGGTG
    AGGTAGCAGACCACACCAGACAGGCTGAACTGACACAGTAGTTTTGAAAAACATAATTTGTCTTTCTATG
    TATTCTACAGTAGCTATTATGTTTTGCACTGTGCAAGACTGGTATGTTGGGTGGAGTGGGAATCGTGAAC
    CAGCGATAAAGGAGGGCTGTTACTCCATTATGTCTCTTCTTCCCAGCATTGGGGGAGGGGGAATGGAGAT
    AGGGAAGGGTAGTTGGTGAAACTAAGTGGCAGGGGGAGATGATGCTCTGAGCAGGAACCCTGGGGCAGTA
    AAGATGGAGAATACAGAAAAAAAAAAAATAGCGGGACACTCTAACTGCAGAGCAAAGACATCCTTTTTGT
    CATCCTCTAGGAATAACAAGATTTTCTAAATATAGCGACAACTAAGAATCAGAAAGCGCAGAGAATTCTA
    CCTACAAACGTTAGAAGACACAAGCAATACATAATAATCTGAAAGGCAGGCCGAGCCCTCGGGCTGGGAC
    GCTCCTGCCAGAAGTCTTGCCTCTGACTGAAGATGGCAGCCACAATATGGGTGGCAGAAGATTCTCAATG
    CCTAGTCGTGGCTAAGACTGACCAGTCTAGACCTGCGTTCTCCAAGAATGCTAAGAAGTAGCTCGGGCTG
    GACCCTCTCCACCAGCGCCCTGCCGTCTGGAATTCGCCCTGCACATGAGAACGGTGCTGACAAGAGAGCG
    CCAGGGACTGAAGAGCCCGCACCGTGTGCTAGGCAGCGCCCGGCGCTGTTTTTCTCATCAAAATCTCAAA
    AGCAGTGGCAAAAGCTGAGGGTGAATAATAGCGAAGAGCCACAAACCCAGTGGGCGTGGAGAGAAACGGG
    CCTGCCCTCTGCTCTTCAAGGAGGGCGAGAATCCAAAGATCAAAGTCTCCTTTTTCTTTTCCACTGGAAG
    AAACCCATCTATCTAGCCTTTAGATAAGACAATCGAGGAGAAGCTGAAGGAATCCCAGGGGGAAAAGGTC
    GAAGCTGCTGGGATACTCCCTGCTCCTCCACCTCAGGCTCCAATCCCACTTTCCCCACCCCCAGCGGCGC
    TAATATCGCTGGAGGTGAGGAAAGGCTAGTGACGCCCTGTCTTGACTAAGAAATGGCACTCTATTGCCCA
    AGACTGCCTAGGACAACCCGAGAAAATCCTGGAGAGGGTGCGTCGTGAAAGCTGATCGGGTTCCCCAGGA
    AGGGCGCCCTAGGGCCGCGCGGGCCAAAGGAGGGGCGGGTCTGGCCGGGTGTGAGAGCTGCTGGCTGACT
    CTTTGTGGCTGGGGCTCCGGACACAGCCCTCCAGCAGACAGACATCCGTAGAGTAAAATGTAGGATTAAA
    TGACTGCCCAAGAAAGGAGCCAACGCTGTAACCAGATCGCCTAGGTGCGGTCCGAAGAAGCCAGCACCAC
    CCTAATTGTGGCACGAAAGGGGCTACAGATAGCATCCTGGCCGCCCGATCCGGTTCCCCTCGTTACCGCC
    GTGCGGCATAAGTGATTCTTTGTGGCCGGCCAATTAAAAAGCTACCTCTTTCGGGGAACTGTTTTGCTTC
    GTCGCCGCTGTGCGCGGGGCTGGAGCCCGGGAGGTGGCGGCAGGCGGGCACGGGGGGTGGGGGGGGGATG
    AGGGCGGGGAGGCTGTGCATTGTGGGAGAGGGGGGTCGGGGAGTTGGGGGACCCACTGGGCCTTGCCTCT
    CATGAATGGGGCTTTTCTCAAGCGCATACAGAGCCCGATTCACAGCTCTCCACGAGGAACCAGTTTAAAT
    AAATACGGGCAGGGTTTCAGCCCATCTGGAAGCCATCGCTGCCAATCCCCCAACGCAAACAAGTCTCACA
    AAGGAGGACTGAGGTCTCCCCTCCCGAACCAGCAGCACTTTTGGAAGGCTAGGGCTGAGAAATGCAGATG
    TGGGTCAATGTCTTCGCGGCCAACAAGTTTCCCAAACTCGTGGAAAGATGGAGAGGAGGAGCGCGGTGGA
    CTCTAGGTTTCTGACATTCCTAACGTCCACACAGGCTACGCCTTGCCCCACGTCAGGTCTTTGGGTCCTG
    TGGATCCAAGGCTAGAGTGTGGGGTGTCTGCCATTGGTGAGGCAAGCTGGGGTGTAAAACCAACGATTTT
    TTTTGGGGGGGGGGGGCATGGTCAGATGCAGAATCGTAAGAGACCGAAAGTTTTAGACAACCTCCCCCCA
    CCCACCCACACTCATACAAAGCTGTGGAATTCACTCTGTCAAAAAGGTTGCAGCAGTGAGAGAGAGCTCT
    TTGACACTTAAGCGAGCGCCTCTGCTATCAGATGAAGAATTCGAGAAAGCGTTAAGACGCTCAGTTCACT
    CCACTGCGCTCCATAAAGAACACCGGTCAAGACCGCAGCTTTGTGGGGTGTGTGCTTGAAAGTTTAGGAC
    AAAAAATACAGTCAGGCCTATCTCAGACCACATACAAACACTTCGATGCCCCCCCACCCCCGCCCCCAGA
    GCTCGAACGCAGGACTCTGGCTTAGCTCGCCACCCTGCCACCCTGCGCGGCGGGGTCGGAAAGTGCTATC
    CCGGGCTTCTCGCGGCAGCGCTCCCCGCTCTAGCCCTGCCCCCCGCCGGGTCCGCCAAGCTAAAGAGCAA
    AAAGTCCGCCCGGGCCCGCCTCCTTCTAAAGCATCTCTGGAGACAGCCTGGTGGCAGGGGAAAGAGCACA
    GAGTCTCACGGGCAGAGGGTCTTTGATCCCCACCTACTGTCCAAACAGTTTCACCTGCGATTGATTAACA
    GTCTGTAACATACAGAGAAAGACAGGGCTGGAACCCTACCCGTCGGAAACGTGCCCAAACACGTGGAAGA
    AAATTCTCTACCTAATACACTTTCCAGGCTTCGGTTGTCCTGAATATAATCCCAGACAGTGGATCTTACC
    CAGGGAGGAGATGGAAAGGTGCGGGAACCGCCCTTGGACCGAGGCTATACAGCTGCCACCTCTAACGATA
    GCCATACGCAGGTCACTAGCCTGACCCCATATCCATCCACGGCTACACACGCTGTAAAGTAACTCATTGT
    AGGGTATACAGTGTGTGTGTGTGCGGGTGGGTGCTCTTGGGGACACATTTGGAAAGCAGGTCTGTTTGCA
    ATGTCAACGTTTTTCAGTGTCAAATTCCTGCAGCCTAACGGAGGCAAGGGGAGAGCCGAGTTTCAAAGAA
    GGGCTGCCCGGCTGCACCTTACTGTGGGCCAGCCTGATAGCGGAGGGGACTGGAGAGCTGCGCTGAAGTC
    GCCGGGAGCCGCTTTTTCTTTGTGGCCTCAGATTTCAACCCATCAGGGTCAGAGTTAGTTCTTTGTCTAG
    CGCCCCTTTGAGTTCTGCAGCGTCTAACCCCTAGACTAGGCTGATAAATCAACCCGTAGGCGTCTCTCGA
    TTTCTCTCCTACTTTTAAGTGTGGGGCTTCTTTTACTGAGCTCGAAACGTCCTGGTCAAAGCTGGTGGAA
    GGTGATGCGTCTGTGTTCAAACCTGTTGCATATCCTTGCTTAACGCAGGGACCTGAGAAAGTCTCCAATC
    GTGACTCTCACTGAAGATGCTGCGACAGAGGTCCTTACTCGCAGAATTAGGATCAGTGTACTCCTGTATA
    CTCTGCGCTTAGGACTTAGCGCCCTAACCCGGACCAGTACTTGGGGGAGGGAGTAGGAGGAAAAGGGTCA
    CAAGTCAGGGAAGTAGGCAGGGGCGGCGGGCGACTTACGGGCATCCTGGCCCTGACACCGTAGGACCGCG
    GCGATGAGCAGCGTCAGCAGCACCAGCGACTGGGGAGCCCCGAGGCGGATCATGGCTCACCGCGAGGCCT
    GGCAGAGCCTGGCCCGGGCGAAGCGCAGCGAGGCGGCAGAGCACGGAGCGGGTCCGGGTCTCTACCGCTC
    CCTCATGCAGGAGGCCCTAGGAGCAGGAGGAGGCAGGAGACCCGGCAGCCCAGCGGCGCTCTGCGTCTTC
    CCCCGGCAGAGGCTCCAGTTATATGCTCCCGGTCGCTCCAAAGGCTGGCAAACCGGCCCAAACCGCGGGC
    CGC
  • Protein Sequence : Show Sequence
    >gi|169658375|ref|NP_001106987.2| collagen alpha-1(II) chain isoform 2 precursor [Mus musculus]
    MIRLGAPQSLVLLTLLIAAVLRCQGQDARKLGPKGQKGEPGDIRDIIGPRGPPGPQGPAGEQGPRGDRGD
    KGEKGAPGPRGRDGEPGTPGNPGPAGPPGPPGPPGLSAGNFAAQMAGGYDEKAGGAQMGVMQGPMGPMGP
    RGPPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGLPGPQGARGFP
    GTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDG
    QPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGSRGEPGNPGSPGPAGASGNPGTDGIPGA
    KGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQAGEPGIAGFKGDQGPKGETGPAGPQGAPGPAGEE
    GKRGARGEPGGAGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLTGPKGANGDPGRPGEPGLPG
    ARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLAGAPGL
    RGLPGKDGETGAAGPPGPSGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKQGDQGIPGEAGAPGLVGPR
    GERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGAAGPDGPPGAQGPPGLQGMPGERGAAGIAGPKGDRG
    DVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPSGSTGARGAPGERGETGPPGPAGFAGP
    PGADGQPGAKGDQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPP
    GANGNPGPAGPPGPAGKDGPKGVRGDSGPPGRAGDPGLQGPAGAPGEKGEPGDDGPSGLDGPPGPQGLAG
    QRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGR
    DGAAGVKGDRGETGALGAPGAPGPPGSPGPAGPTGKQGDRGEAGAQGPMGPSGPAGARGIAGPQGPRGDK
    GESGEQGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGSNGIPGPIGPPG
    PRGRSGETGPVGPPGSPGPPGPPGPPGPGIDMSAFAGLGQREKGPDPMQYMRADEADSTLRQHDVEVDAT
    LKSLNNQIESIRSPDGSRKNPARTCQDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNP
    ATVPRKNWWSSKSKEKKHIWFGETMNGGFHFSYGDGNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIA
    YLDEAAGNLKKALLIQGSNDVEMRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPM
    DIGGAEQEFGVDIGPVCFL
  • Molecule Role : Protective antigen
  • Related Vaccine(s): Adjuvant arthritis DNA vaccine CII encoding type II collagen
4. hsp65 from M. leprae
  • Gene Name : hsp65 from M. leprae
  • Sequence Strain (Species/Organism) : Mycobacterium leprae
  • NCBI Protein GI : 34333423
  • Other Database IDs : CDD:213108
  • Taxonomy ID : 1769
  • Gene Strand (Orientation) : ?
  • Protein Name : heat shock protein 65
  • Protein Length : 201
  • Protein Note : chaperonin_like superfamily. Chaperonins are involved in productive folding of proteins. They share a common general morphology, a double toroid of 2 stacked rings, each composed of 7-9 subunits. There are 2 main chaperonin groups. The symmetry of type I...; cl02777
  • Protein Sequence : Show Sequence
    >gi|34333423|gb|AAQ64514.1| heat shock protein 65 [Mycobacterium leprae]
    EDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVKEGLRNVAAGANPLGLKRGIEKAVDKVTETLL
    KDAKEVETKEQIAATAAISAGDQSIGDLIAEAMDKVGNEGVITVEESNTFGLQLELTEGMRFDKGYISGY
    FVTDAERQEAVLEEPYILLVSSKVSTVKDLLPLXXXXXXXGKSLLIIAEDVEGEALSTLVV
  • Molecule Role : Protective antigen
  • Related Vaccine(s): Adjuvant arthritis DNA vaccine pCMV3.65 encoding HSP65 , Pristane-induced arthritis DNA vaccine DNA-hsp65 encoding Mycobacterium leprae 65-kDa heat shock protein
1. Ifng (Interferon gamma)
  • Gene Name : Ifng (Interferon gamma)
  • Sequence Strain (Species/Organism) : Mouse
  • NCBI Gene ID : 15978
  • NCBI Protein GI : 33468859
  • Genbank Accession : NM_008337
  • Protein Accession : NP_032363.1
  • Other Database IDs : MGI:107656; UniProt: P01580
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • DNA Sequence : Show Sequence
    >gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
    ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
    GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
    GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
    AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
    AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
    GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
    AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
    GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
    AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
    AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
    AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
    TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
    TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
    ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
    GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
    AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
    TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
    AGTATACCTTTGCAACC
  • Protein Sequence : Show Sequence
    >gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
    MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
    ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
    LLPESSLRKRKRSRC
  • Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
  • Additional Molecule Role : Vaximmutor
  • Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
  • Related Vaccine(s): Adjuvant arthritis DNA vaccine CII encoding type II collagen
2. IgG (partial)
  • Gene Name : IgG (partial)
  • Sequence Strain (Species/Organism) : Rattus norvegicus
  • NCBI Protein GI : 204718
  • Other Database IDs : CDD:209398
  • Taxonomy ID : 10116
  • Gene Strand (Orientation) : ?
  • Protein Name : immunoglobulin gamma-1 chain
  • Protein Length : 106
  • Protein Note : Immunoglobulin domain; cl11960
  • Protein Sequence : Show Sequence
    >gi|204718|gb|AAA41373.1| immunoglobulin gamma-1 chain, partial [Rattus norvegicus]
    RTQVPHVYTMSPTKEEMTQNEVSITCMVKGFYPPDIYVEWQMNGQPQENYKNTPPTMDTDGSYFLYSKLN
    VKKEKWQQGNTFTCSVLHEGLHNHHTEKSLSHSPGK
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Adjuvant arthritis DNA vaccine pcDNA-CCOL2A1 encoding type II collagen
3. Il10 from Rattus norvegicus
  • Gene Name : Il10 from Rattus norvegicus
  • Sequence Strain (Species/Organism) : Rattus norvegicus
  • NCBI Gene ID : 25325
  • NCBI Protein GI : 148747382
  • Genbank Accession : AABR06074649
  • Protein Accession : NP_036986
  • Taxonomy ID : 10116
  • Chromosome No : 13
  • Gene Starting Position : 52824593
  • Gene Ending Position : 52828559
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 10
  • Protein pI : 8.68
  • Protein Weight : 19532.21
  • Protein Length : 178
  • Protein Note : Also known as IL10X
  • DNA Sequence : Show Sequence
    >gi|389675116:52824593-52828559 Rattus norvegicus strain BN/SsNHsdMCW chromosome 13, Rnor_5.0
    TCGGTATAAAAGGGGGACACCGGGCAGGAGATCTACACATTAGGGACTTGCTCTTATACTACCATAGCCA
    CAACGCAGCCTTGCAGAAAACAGAGCTTCAGCATGCCTGGCTCAGCACTGCTATGTTGCCTGCTCTTACT
    GGCTGGAGTGAAGACCAGCAAAGGCCATTCCATCCGGGGTGACAATAACTGCACCCACTTCCCAGTCAGC
    CAGACCCACATGCTCCGAGAGCTGAGGGCTGCCTTCAGTCAAGTGAAGACTTTCTTTGTAAGTATAATGC
    CCACCTAGCCTTTCTTCCTAGGATCAACTGAAATATGCATTCTGATGGAACTGTAGCTCTCCTCCTCCTC
    CTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTTCTCCTCCTGCTACTCCTCC
    TCTATCTCCAGCATCTATTACCCTTAAACTTGAATCCAGAGAGTCCTAGGCCAAGCCATGGGTTATGAGT
    TAAACTAAATCCAGGCATAGCTGAAAACTAGGTTAACTAGCTAGCTAGCTAGCTAGCTAGCTAGGTTAAC
    TCCTAGCTAGCTAGGAGGTGAATGCATTGACTCTCCCATGCTCAAGAACTTTCTGTTAAGTTTCCGATGA
    GACCCCATGTTTTGCTGGCTAGGACAAAGCCTGGGGTAGAAGATACATGCTGTCTCTGCTTAGTCTCAAG
    ATCTGGAGGACCAAAGTCTGGGGGTTTGGGGGAACACCAGAGCTCAGGAAACTGCTGTAGGAGGTCCAGA
    GAAGGCAGAGAACCATGCAAAGCAGAGGCTTGCCGTAAGGGACTCCAGACAGGAAGGCTTGACACAGGTG
    CCCTAGAAACCTCAACTGACTCATCTGAAAGAAGACCAGGCCTGGGTCAAACTTCTCCCAATCTGTTAAA
    TTCAGTCAGAAGTTTTTAAAAACAACTTGATCCAAAGAGGAAGGACTTTGATTTAACTCCGGGCTCTAGC
    ACCATCTAATCCTGTTTTTAAAACTCACCTTTCCAAAGTCACCAGGGCTACCTTGATTACAAATTCAAAA
    CTCTACAAATGGAAAAGGTATTCAAATGGCACGCGTGATGGGTATTCCCCCAGCCCTTGTATTCATGGTC
    CTATTGTCCCTCCAGCAAAAGAAGGACCAGCTGGACAACATACTGCTGACAGATTCCTTACTGCAGGACT
    TTAAGGTAAGAATCCACTGGGTGCTGGGGAGCAATTAAGTGATTAGGGAGGGGAGGTGCTGCTTGTGACA
    GAGAGAGCTGGGTCTGGTGCCGTGGCTTTCAAAAAGAGAAGTGAGAAGACCTTAACTCAGCATATTGCCA
    GCAGTCGAGGGTTATGAAGAGCTCATTCTGTGGTAAAGAGAGAAAATGTAACTGGGGGGGGGGGGTCCCC
    TAGGGACAAGACTGTCCATCAACATTAGTGGCAACAGTCCATCCTTCCAATAAGCCACACCCAACCTTTG
    ATCCCTGCCTTTAGGGTTACTTGGGTTGCCAAGCCTTGTCAGAAATGATCAAGTTTTACCTGGTAGAAGT
    GATGCCCCAGGCAGAGAACCATGGCCCAGAAATCAAGGAGCATTTGAATTCCCTGGGAGAGAAGCTGAAG
    ACCCTCTGGATACAGCTGCGACGCTGTGTGAGTAGCAGACCAGTTCTTTCCCCTGTAGCCACCCAACAAA
    CACTGTCTCCTACAATCCAGCCAGGCCATGCGCATCCAGACACACACAGACTAGACAGAGGACTAGGTAA
    ATCTAGAGGGACACCCGGAGTCAGATCTCTTGCTTGTCTGTCTCTGGGCGAGAGTGGGGGTGGCTTTGAG
    GCGATCACACGTGAAGATTTGCGTATAGCCTTCCTGTTATTTGTGAGTCATTGTGGGTCATTAGCTACTC
    CCCTCTCTTCAAGGGAAGGCTAGGGCTTCAGTCATAGTTCCCTCGTGTTGGGGCTGCCAGCCAAGGCTCC
    CCAAGCAGAGGAAAACATTGGTCTCTGTATCTCCTCTTTTGGAAAGCAAAGTCAGTGCCACACACCTGGG
    CTGATAGAAATCTCCCCGGTATTTCAGAAGGGCCAGAAGACTACTGTCTCTAAGCCAGTATCTATGAATA
    GCATATCCAGGACATCTATGTCTCTGACTGAAGTGATTCCTTCGCTGCTGTTGTTTCTTCTTTCTTCTCT
    CACCGATACTGGAAAATCAGTTGGAGTCTACCTGTGCCCAACGATGGTTCTGGCCCAACAATTTGGCGCC
    AGTAACTGAGGCTTTTTCCGATCCACTCTCAGACACCTAGAAATAGCTCATGGTGGTTGAGATTGGAGCT
    AGACAAGGAGAGACATTAAAAACATAACATGTTTTGGGGCTGGCTCGGAGGAACAAAACCCAAGACCTAC
    CTAAGTTCACACCCACGCTCAGCAAACCATGATTTCAAAGACATACATTGTATCTTCTTTAGGCTGTTTT
    CCTCGGCACAGAAAGATAAAGGGCCAATCATGGCTCAGTTCTCCCTGGGGGCTAGGAAGTAGATATCGAA
    GATAAGCAAGGGTAGCTCCTTCCTCATTACAAGAAGATAGTTTCCTCCCAAGAGCCATGGATACAGGGTT
    CGAGCAAGAATTATCAACACTCTTTTTTTTTTTTTCTTCTACAGCATCGATTTCTCCCCTGTGAGAATAA
    AAGCAAGGCAGTGGAGCAGGTGAAGAATGATTTTAATAAGGTAAGCCTGGGCCATGGCAAAGAGAACGCG
    TGGAACGTGACCTCCGTCCGTCCACTCGCAAGGGCAGAAAGAGGGCCCGACCTATTCCGACCGAACCTGG
    AGTGTGGGAACCAGCAAATGGTGTGACCTCCTCTGCCAGTTAGAAAGCCACCACCTCAGTCACATTTGTT
    TTCTGCAAAGCGTCTCTGGCAGTTTCTAAATGACTGCTCCACTTTTGCAGGCTTTTGGCTTAGACTGACC
    AGACAGCCTATAAGCGCAGAGCGCTAGGTGCTGGGGAGAGCGCCGTAGGAAACAGAAAGTACAGAAAGTA
    CCTTGTTGGGAAACAGGCAGAACCCACAAGTACAGAAAGCAGACATGCATGAGTGCACGCAGGTACATCT
    TGGGGCTCAGCCTGGATGCTCCTCGGAACCAGAAGGAGCAGGAAGCAGAATTCTTAGGGGTCCCAGGGCA
    CTCTCCACCTGGCAAACAAAATGAGGTTTCATACTTCCTTCTGCCTGTGAACCTATTCAACCCTAGTTCC
    CAGAAGCCATGTGGCCCACATCTTCTTTGTGGGCTAGGCAGAGCTCCAACATCAGTGGGCATGGATTCCA
    TGACAGCAGGCCAGAGCAGCGGGGTGAAGACTGCCTACACACGCAAAACATTAAAAAAAAAATGTTTTTC
    CTTGTTATCTCTATCACTGTCCTGCTTTGAAATTGCTTCCTCTAGCATCTTTGCATAACATTCTGCATAC
    GCATTATGTGGGCACTGGCCTCTGGGGGGGGGGGGGGGAGTTGAAAAGGCTCTACTCTGAAGACAGTGCT
    TTGAGAACCGAATGCTTCCCTTTCCCTACTGACCCCTGGCAGTGCTTAAAGTCGGAGAAGGTGCAAGGGA
    AGACACAGCCCCTTCCCAAAGCAGATGAACAAATCTGCTTTGGGTCCTTCAGCTCCCAACACAAGAAAGC
    TGAGATCTTTCTACACTCAGTGGCCTTCCTAGTGCAGGGAGCCCCTCAGCCCTCCAATGGGTCCTAACCA
    GATTCTTCTCTCCCCACACAGCTCCAAGACAAAGGTGTCTACAAGGCCATGAATGAGTTTGACATCTTCA
    TCAACTGCATAGAAGCCTACGTGACACTCAAAATGAAAAATTGAACCACCCGGCATCTACTGGACTGCAG
    GACATAAATAGAGCTTCTAAATCTGATCCAGAGATCTTAGCTAACGGGAGCAACTCCTTGGAAAACCTCG
    TTTGTACCTCTCTCCAAAATATTTATTACCTCTGATACCTCAGTTCC
  • Protein Sequence : Show Sequence
    >gi|148747382|ref|NP_036986.2| interleukin-10 precursor [Rattus norvegicus]
    MPGSALLCCLLLLAGVKTSKGHSIRGDNNCTHFPVSQTHMLRELRAAFSQVKTFFQKKDQLDNILLTDSL
    LQDFKGYLGCQALSEMIKFYLVEVMPQAENHGPEIKEHLNSLGEKLKTLWIQLRRCHRFLPCENKSKAVE
    QVKNDFNKLQDKGVYKAMNEFDIFINCIEAYVTLKMKN
  • Molecule Role : Other
  • Related Vaccine(s): Adjuvant arthritis DNA vaccine pcDNA-CCOL2A1 encoding type II collagen
4. TGF-B from Rattus norvegicus
  • Gene Name : TGF-B from Rattus norvegicus
  • Sequence Strain (Species/Organism) : Rattus norvegicus
  • NCBI Gene ID : 59086
  • NCBI Protein GI : 11024652
  • Genbank Accession : AABR06003977
  • Protein Accession : NP_067589
  • Taxonomy ID : 10116
  • Chromosome No : 1
  • Gene Starting Position : 83742150
  • Gene Ending Position : 83758471
  • Gene Strand (Orientation) : +
  • Protein Name : transforming growth factor, beta 1
  • Protein pI : 8.63
  • Protein Weight : 42045.82
  • Protein Length : 390
  • Protein Note : Also known as Tgfb
  • DNA Sequence : Show Sequence
    >gi|389675128:83742150-83758471 Rattus norvegicus strain BN/SsNHsdMCW chromosome 1, Rnor_5.0
    TCAACCTCAAATTATTCAGGACTATCACCTACCTTTCCTTGGGAGACCCCACCCCACCCCACAAGCCCTG
    CAGGGGCGGGGCCTCCGCATCCCACCTTTGCCCGGGGTTCGCGCTCTCCGAAGTTCCGTGGGGCGCCGCC
    TCCCCCATGCCGCCCTCGGGGCTGCGGCTGCTGCCGCTTCTGCTCCCACTCCCGTGGCTTCTAGTGCTGA
    CGCCCGGGAGGCCAGCCGCGGGACTCTCCACCTGCAAGACCATCGACATGGAGCTGGTGAAACGGAAGCG
    CATCGAAGCCATCCGTGGCCAGATCCTGTCCAAACTAAGGCTCGCCAGTCCCCCGAGCCAGGGGGAGGTA
    CCGCCGGGCCCGCTGCCCGAGGCGGTGCTCGCTTTGTACAACAGCACCCGCGACCGGGTGGCAGGCGAGA
    GCGCTGACCCGGAGCCCGAGCCCGAGGCGGACTACTACGCCAAAGAAGTCACCCGCGTGCTAATGGTGGA
    CCGCAACAACGGTGAGCTCTGAGGGGCTGGGGAGCCAGGAGGGAGCCCCCAGGGGGCGCCGGAGTGCAGG
    GGTCACCGGGAGGAAATTACCCGCAGAGGAAACTGGCTGGAGGAAGGGGACCCCTGGGGGCGCTGGGATG
    ACTGTTTGGGTGTCCCAAAGAGGATGGCGCTTACCCCTACCCCAGGTGTCTGGTCTTAGAGAGAAAGACA
    GCCTGCAAAAGTGAACCTCCCCTAGAAAGGCATGATCCTGGGGAGAGTCCACTCTAGAAGAGGAGCATTT
    GTCAGGTTTTGTTTTAAGTTCCCCTTATGGTGCCTGAAAATGCAAGGCTCGTTGGGTCCTTTTCCAGAAA
    GGTAAAGCCCGGCTCAGGAAAAATGGAAGGCGCCCAGCATTTGGGAAACAAGAGACAGTGTGGAAAGCCG
    ACACTAGACCGGGTCGTGAGATGGAGAGAAGAGATTCAAGGGAAAAAAAAAACCTAGGGGATGGGTACCC
    TAGAAATGCCAAATAAAATCTCTACTGGGCTTACTACCAGAAGGCGACAGGCGCGTGCTGGAGGGTGTGA
    TCTGTGGAGTCATTGTGGGGTTCCAAGTGATAGAGGAAAGCGAAGTTGTTAGGAGAAGGAGGCTAGAGAG
    AGAGGAGCGGAGAGGGTGCGGAAAATGAGCCCGGAGGTGTCACGAGTGTTTTGCTTGGGGGCGTAAAAAA
    GCTTGCGAGCGGGCAGGCGGAGCCCAGTGGAGGAAGCGAGCGAGTCCTCAAGAAATTTAGAATACTGGGT
    CATTGGGAAGGAATTATAAAAGAGGGGAGACTGGCGTAGGAAGGTGGCCCCGCGAGGCGGGCAGGCAGAA
    CAGGGATACTATGCCATCCAGGCGAGAGTGGCGAGCGCCCCTCGCCTGGGCGTGGAAGGGAGTGGCTGAA
    CCTCGGGCCCTAGGGGAGCTGCTAGAGGTAGGAGAGAGGCAGTCTTCAAAACCCAAATGAAAAAAACGAG
    TCGGTAAGGGTGAGGAATCTCCACGTGGGTGCCACGCGCGTGGGGAGGAGCTTGCGCTTCTTGAGGGGGT
    TAGAGGACCCCACGGAGCTGAACGCTGGCAGGCTGTACCCCCAAAGTCCCAGTTCCTTCTACGGGAGGCT
    GGGGAAAACCCAGCGTCCGGTCGCCTCGCCCTCCTCCCTTCCCTTTCCTTTCCGTGCCCAGGAGGAGGGG
    GGAGACTGAGAACGCGCGCGCGGAGCCTGGGGCGAGACGAGGCAGGTCCGGATCCCGCCCTCCCTAGGCT
    GTTAGTCTCCCGCCCTCCTCACCCCAGCAGTGGGCGGGGACACTCACGGCCTCGCCCTTTAGTCACCCAC
    GCGGGACCTAGGAGTCCAGTGTGACCGGGAACTGTGGGTGTCAGAAGCTTCTCCAGGTTTCACCGTCCTA
    ACCCCCAGAGGTGGACTTAAGAATTCCTTGCACATCGCTGAACCCAATTGTGGCTCAGGCCTAATGACAC
    CCCTCCCTAGTCACCATTTAATGTGACCTCTTCGTTAAAGTCTTTTCCTCTCCCCTAAGACTGTATTTCT
    TCTCTTACGCATATATCTCTTCCGGCATGTGCCTCCCCGTTTCCGTCTCCTCTCCCACGTATCTATTCAT
    CTCTATGTCACCCCACTTCCCTCTGCGACACCCACAATCCTTCCTGCCCTCTTATCTCTCCCCTATCTGC
    TACCCCCAACTGAGATCCCACCCCCTCCATTGTGGTTTGCTGAGTAACAACTGGTCCAAGAATAGGGCCC
    CTCTGTCCTTCATCGATGTCTGGGTCACCATCTCATCCCCACTACAGGAAGTCAAAAGCTCCAGTTGATG
    GAGGATTTCAGGACTTTGCCCCTTCGTCCACCGACGCAGACATCCTTTAGCCAAGGAAGCCCTTTAGTGA
    ATGGGGCTCCCAAACATGCAGTTATTCTTGCCTAGAAATCAACCTCGCTACTAACCCGGCCAGGAAGTGG
    GCTAAAGAGGGAAGTAGCCGATGATAGGCAGATGATAATTACCCTGCACTGGCCAGGAAGTAACAGAGTT
    TCACGATTAAGACCACAGTCTGGTTGTCTCTACACCTGATTCCCCACCCCCCCAACTCCCCTCTCCCACT
    CAGTTCTCTCCCTTCATCACCTCTGATGGCTTCCTGACTTGATGTGCAGGGCTGGATTGGTACCCAGCGG
    TGAGGGAAGGGGCTGTGTGAGGGGGGGACTGGAAAGAGGGAAATCTTCCGTTGGGATGAATAAATAAATA
    AGTAAATAAATAAGTGGGGGGGGGGACAAACCACCATCTGCCAAGCAGAGCCGTCCATGACTGTAGTCCC
    AGTACGAGGGAGATAGAGGCGAAAGGATTGCTTCGAGCCTAAGCTATGAGATCCTGTCACCAGAAACAAA
    AGAAAATAAACAAACAAAAAATTAGCCCCTAGCATAAGATGGCTTGAATCTTACCACATTGTGAAGTCTT
    GATGTCTTTATCTTTGTAGTGTTTAAAACAATTTCGTTACCATTCACTGTATTCCCCTCACCCTTATTCA
    CACATGCATGCACGCATGCACGCAGGCAGGCAGGCAGGCTAACTCCCAGTGAACAGCTTCCTGACTCGGG
    CTCCTGAGTGCTGCTGGCTGCAGACTCGCACCGGCTAAAATACACATTATCGACGTGTGTGTCACATGTT
    TAGCTTTTGCCCCATGTCTTATCAGCTCCCTGGAATTGGATGGTATCTTGCAGTTAAAGAGAAGAATTTG
    CTCACCATTGGGTACATAGTGCTTCAGTTGAGGCCACCTCAGTTGGGAGTCAGAACTTAATGGTACCTGT
    TTCTTCCAAGGTCAGAAGGAGATTGGCCCAATGGGAGAGCATTTCCCTAGAATCCACCTTTGAGGTGTTC
    GCTCCAGCACAGCTGTTCGTTCCAGCCTAGAACCCGCTGTGGAAGTCACTCAGTGCCACTTTCTGGTCCC
    CACAATTATGCTATGCCATTCACTCTACAACTGCTGTGTGGTTTTGTTTGAGGTGTGGTTTTGTTTGAGG
    CGTGGTCTCGCAAAGCCCAGGTGAGGTCAGCCAGTTAAGAGCATTAGAGAGGGGCTCAGCAGTTCAGAGA
    ACCAGCTGTTCTTCCAGAGGTCAGCCGGGGCCCACATGGCTGCTCACAGCCCTCTGTGGCTCCAGTTCTA
    GAGGATCTGACGCCCTCTTCTGGCCTCCACAGGCGCCAGGCATACACTCAGTACACAGACGCACAGACAC
    AGCCGAGAACACCCATAGACTTAAAGTTAGAAGAGTGGACATGTCAAAGTGGAGCGCCAACCTGAGTTAG
    AGCCCATGGGCTCAGAAGCAGGGCACCTCTTTTTCTCCCATCCTCGCCACAGACCTGATGTCTTTCTCCT
    TCTGCTCCTCCCCACCCAGCAATCTATGACAAAACCAAAGACATCACACACAGTATATATATGTTCTTCA
    ATACGTCAGACATTCGGGAAGCAGTGCCAGAACCCCCATTGCTGTCCCGTGCAGAGCTGCGCCTGCAGAG
    ATTCAAGTCAACTGTGGAGCAACACGTAGAACTCTACCAGGTGAGGCTTTGAGCCTGAGAGGTGGCTCAC
    AGGTCACAACTGCTGAGGTGTGGCCAGGTGGCTCTGTTTGACAACTACAGGGCACACAGCCAGTCCTTGA
    GGAGAGAATACAGAGTGTTGAAGCCTAAGGGTCTTCTTGTTTTCTTTTGGTTGGTTGTTTCGGTTTTTCA
    GGACAAGGTCTCTGTGTAGCCCTGGCTGCCCTAGAACTCACTCTGTAGATCAGGCTGGCCTCAAATTCAC
    AGAGATCCATCCACCTGCCTTTGCCTCTCCAGTGCTGGGACTGAAGGCAGGTGTGTGTCACCATAGCCCC
    CGAGAATCCTAAGGTTTTTAAAAAATGCTTAGATTTCGTCCCATTAGTTGAATTTGGGATGTTGAACTAA
    AAATGCAGAAAGAGCCATGGGATGGATATCTGGATTTGTATGCTTGCCTAGAATCCCCCAGTGAGGGGCT
    AGGGGCGTGGCTCAGTGGTAGACTGCCTAGCATGAGTAAAGCATGGGGGTGGGAGTGGGTGGGTGAGGAG
    AGGGGATAACAGTGAGGAAGGAAAGGAAGTTGGCAGACACTTGCAATCCCAGCACTTGGAGGATGGAGGC
    AGGAGACCAGGAGTCTGAGGCCAGCCTGAGCTACTTGAGACCCCGTCTGGAGGCTGAGGCAATAAGATCT
    CTTGAACCCAAGTATTCAAGTCCAACATGGAATAGTGAGATCTTGTCCCGGACAGAGAAATTAAGCATTT
    GAAATGCCCTTGTAGTCTCTTAAGTCAGCAATCACCATTCTGGACTTCAAAAGTAGCTTCTTTTTTTCTT
    TTTAAAGACAGGGGTTCTCTGGGTGTCTTGGAACTCAGCAATCCACTCACCTCTGCCTTCTGAGACTTGG
    GCCTTAAAGGCTTGGGCCACCACTGCCAAGCCAAAAATAAAAATTTTAAACCAGGAGGCAGAGGCAGGAG
    GGTCTCAGAGTTCAAGGCCAGCCTGGTCTCCATTGTTAGCTCCAGAACAGCCAGGGCAACACAGAGAGAC
    CCTGTCTCAAAAAACAACAATGACAAAAACAAAAACATTGGGAAGACTGTTTTGTGAGTGTACTCATAAT
    AATAATTGTAAGTAAGATAACAAACTAAAGATAGAGAGATAGATAGATAGATAGATAGATAGATAGATAG
    ATAGGAAAGAGAGTCACCACTCTCCCAAACTCAGGATAGTAAACCGGTTAAACTGTGCTCCACAGTATGA
    GCAATGAGCACTTGCGTTGAGGGCCCTCCCAGCTGCTCAGCAGCGTCCACCATGGAAGAGATTGAGGTCC
    CGCAAGGCTGTTGAGTGCCCATAGCTTGCACAGAATCTCATGCCCAAGGGGGAGCCTCACGACTGACCAC
    AGCCTGGGGTTGTCGTTCCTGTCCCATGCTGTGTTCCAGTCTCTGTCCACCCCTCCACCCCCACTCCTGG
    CTACTTTTTGCTCTTGGGTGCTGTATGTGAGGGCGTGGCCTGCAGGATCCAGAGTCTGTACGTGTCAGAA
    GCCAGTGTCTGGGGGATGCCAGTTTGTGGGACAGGCAGCATGGGTTTGCAGCCAGATGATCTGGGGACAA
    GATGGCAGCCACCATACTCCTCTTTGCTTTCTTTGTTTCCATTCAGAAATATAGCAACAATTCCTGGCGT
    TACCTTGGTAACCGGCTGCTGACCCCCACTGATACGCCTGAGTGGCTGTCTTTTGACGTCACTGGAGTTG
    TCCGGCAGTGGCTGAACCAAGGAGGTGAGATTGCTTGTCTTGTACCATCCTCCAGTGGGCCCCCCCCCGT
    TTGTCCTGGCATCATTGTGCATAGCACTGCATTCCCCCCAACTGCACCCCTAACACACACACACACACAC
    ACACACAAACACACACCACACACACACTCACACACACACACACACACACACACACACACCACACACACAC
    ACACACACACACATACCTGGTACTGATGTCAAGAAGTAGTACTTCACAGTTCTTACCAATGGATCAATCT
    GTGGAGGGATGCTAGGGATAGACGGTGACCCAAACAGTCCCTATCCTTGCCTTCAGTAGACCGAGTGGTA
    CCTTGGACAGTTGAAGGATAGGTTGACTTTTAGCTGTGCTTTATGACTCATCGTAGAGAGGAACGTGGTT
    GAGCACTCAGGAGGTGCCTGACTGTCTGGATTCAAGGAGGGCTTCCTGTAGGAGAGGGCATCTGAGGTAA
    TGTCTCTGAGATGAATAAAAGCAAGCAGGAAAAGGAGAAATGAGAGTATCCTGTACCAAGGCTCTGCAGC
    CAGGGAGAGAGCCTGCTGTGTCCGTTTGTCCAGGAGTGAACAGTCTCCCTTGTCTCTTGTGGGTCCTTCC
    CTTGCAAGGCCCACACGATCTTTCCAGACACTGGTAAGGTCCTGTCAGAGCCCTAGCAGGCAGTAATGGC
    CTATAAGGATGTAGGCCAGAAAGGGAGACCTACAGAGAGGGCAAGGCTTAGATGAGGGAAACCAAAGTGT
    TGTGGAAACCCAGGCAAGGTTAGGACCCAGGAGGGCTTCTGGGATGAGAGAAGACTGACTCATGGGATGT
    GTAGTTTAGCCACATGGAGGATGAGTCCGAGTGGGCTGGCACCAAGAGGAATGTGGAAAGCCCACTGGAT
    GGAGGTCAAGAAGAATTTTGTGTGGTATACAGTAGGCGCTTAGTATCATTTATGTGTCCACTCAAGGAAC
    ATCTCCCTTGAACCAGTAAGATGGCTCAGCAGATAAAGGTGCCCACTGCTGAGATTAATGGCTTAGGTTC
    AAGCCCCAGGAACACCCAGGAAGTTGGAGGGAACCAACTTCTACAAATTGACCCCTGACCTCCCTGCATA
    CCCTGGCACATGTGCACCCCCCTGAATAATGAGAAGAGGCCAGCTTAGGTTTGCACCGTGCCTTTGCCTC
    TGGCTCCCTGTGTGGCCCTGGCTAAGTCGCTTCCTCCTGGAGTGAAGGACTCTGAAGATCAGTGGAGTTT
    AGATCAGTGAGTGACTGGCGTCTGTGTTCGCTCTGAATGTCCTTCGACACCAACACTGCTCCTGGCCCTT
    GGCACCTAGATCATGATGATGTTTATCTTTGTGACCAAGGACCAGGCTTTACAGAGCAAGAAGATAGCAA
    GAACCCAGGGCAACATGCCTGATGGTACGAAAGCCTGCTAAACTCTGCGCTTGGGAGGCGAAGGCAAGAG
    GATCATGAGTTCAAGGTCTTAGCTCCATAGTGAGTTCGAGAATTTGAGGCCAGCTTGGGCTACGTGAGAC
    CCTGTCTCCAAAGAAAAAGAGGGCCATGGAGAGGGGTCATGGGCGCAAGAGCTTCCATTTTGCAAGCACA
    AGGACCTGAATTCTCATCCCTGGCACTCATGTAAAAGTCCGACATTGGCGTAGTGTGTGCCTGTAACCCC
    AGCCTTGTTGGGGGTTCGGTGTATGTATGGAGACAGGCCAACTCATGACTCTGTGATCTGCCAATCTGGC
    CAAAGGGGTGGGCTCAAGTCCAGAGATCTGTCTGAGGCAATGAGGGAGAAAGGATTAGAGGAAGATGCTT
    GTCACCCAGGTATAAGCCTACCTGGTATAAAATGTACATTATACATGCATACATATACATATATACACAT
    ACATATCTACAGTTCCAAAAAATATAAAATGAAAGGAAATAAGGCAGGCACAGGATCCCTCCTCTGTTCA
    TGTTGTTCTACTGAGAATAATCAAAGAAGTAAATACTCATGCAGAAATGTCAGAGCCCCTGCCCGCTATG
    GAGGAAAGGAATGGATGCCACATTTGGAGAGGGAAGTGTCTCCTCAGAGCCTTTCCCAAGGGGTTGACGT
    TTGAGCTGAGCCCTGAATAAAGGGGAGAAGGAACCAGCAACTTAAAGAGCGAAAAGTGTGGGGTGACTAG
    CAAGGAGGCCCCAGGGCTGGCAGCTTCCTGCCCCCTCTCCCTTCCCCCCATCAAGGCCTCACCAGTCACC
    TCAGACTCCTTTCCTCTCCTGCAGACGGAATACAGGGCTTTCGCTTCAGTGCTCACTGCTCTTGTGACAG
    CAAAGATAATGTACTCCACGTGGAAATCAATGGTGAGTTAACTTCCCAGCTCCTGCCGGACACAGACCCT
    GCATGTGCGTGCGTGCGTGCGTGCGTGCGTGCGTGCGTGTGTGTGTGTGTGTGTGAGAGAAATCTCACTC
    GCCCCTTGCTCACCCATCCATCCCTCCACAACATTCAAACCCAAACAGGGATCAGTCCCAAACGTCGAGG
    TGACCTGGGCACCATCCATGACATGAACCGACCCTTCCTGCTCCTCATGGCCACCCCCCTGGAAAGGGCT
    CAACACCTGCACAGCTCCAGGCACCGGAGAGCCCTGGATACCAACTACTGCTTCAGGTAAACTCTGTCTC
    TCTCGGAGGCTGCACAGGCTGGGGGCTTGACTGCAGCCTGTGGACTGGTGTGCCACCATTCATGCCTGGA
    GGGCTTGGGGACAAGTTCTTGGTTCTGTGGGCAGCTGAGAGTCTAGAATGTGGGGTGTTTTCTGGCTGGA
    GTGTTCAGGTTAGCACAAGAGTTAGAAGTCGGTTGGAAGGTTATCTTGTTGGGTTTTAAAATTCTTGCTA
    GGCTTCCACCACTTTGCAATGCTAATGAAAGAATTTACTGTGAGCAATTGAGAGTGCTGACCCTAGAAAG
    CGGGAGAGTTGAGATCCAAGCATTTGGGTTCAGAATCAGAAGGCGGTCTGCACTGGGTTCCCACACTCGA
    GGCAGATGGTGGTCTCCAATGCTGGTAGTTGGGAACGACAATTATTGGGGCTCAGAATGGCAGGGTCTGG
    CATGGAGATGTTAGCGTTCAGAGTGGAGGTGGAGAAAGCCAGAATGTGAGTCCCCTGAGGGTCAGGGCTT
    CATCTTGCAGTGGTGCACCCTGAGGAAGCTGGGAGGCGGAACTTCCTGAGATGTAGGCTTACAAGTGTGG
    AGTGTTGGAAGACAGCACTGAAGCAGGGAACTCTGAGGAGTCGGAGAGTGGGCATCCTAGAGTGTCCAGA
    ATGGCCATCCCAAGGGCAAATGCTTTTTGGTCATTGTTTTTAAATTATATCTGTGTGTGCGTGTGTGTGT
    GCGTGCTTGCGTGTAGGGGCACCCATGCCATGACACTTGTGTGAAGGTCAGAGGACAACTGTTGGGAGTC
    CGTTCTCCTTCCACCGCGAGTGTCCCAGAGATAAGACTCAGGTTATCAGGTTTGGCAGTGAGCACCTTGA
    CCCACTTTGTTTTGTTCAAGACAGGGCTTTGCTGTGTGACTGAGGCTACCCTCAGATTCACTCCTTTAGC
    CCAGGCTAGCTTGAATTCTCTGTCCTGGGATTACAGGTGTTACCCAACCACCCCATTTCAGACAGAATGG
    CAGACTCATACTAAGCCTCGGTAATGCGGATTGCGAGAAAGAAGTTGAAACACTAGATCCTAGGAGGTGT
    GAAATTCAGGAGAGTTGAGATGAGAGCAGTGAGCTACCTGTCCCTTCCCTGCTTCAGACTTTATAGAGTG
    CTGTGTTGTATGTCGGTCCTTGGCTGGAGCTGCTCCTCCCCCTCCCCCACCTCCACCTCCTCCTCCTCCT
    CCTCCTTCACCGTCCCTTTTTTTTTTTTTTTTTTTGGTTCTTTTTTTCGGAGCTGGGGACCGAATCCAGG
    GCCTTGTGCTTCATAGGCAAGCGCTCTACCACTGAGCTAAATCCCCAACCCCCCCAACGCCTTCACCGTC
    CTTTTGCTGTTAGCCTTCACATAGGAACAGATGGAGGGGCTTCACACTGCAGGTTACTAAGAGATGAGAA
    AACTATGCAAGCTCTGTTCCCCGCATACTGGGAAACGAACTTAGGGAATCAGGAAAATCTCTTTGTCTGT
    CTGTCTGTCTGTCTGTCTGTCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTCTCTCTCTACATTTGTTTTG
    TTTATGCCTGATGTATGTGTGGGCTCTGGGAATCCAAACCCAGGTCTTCATGCTTGCATAGCAAGCACTT
    GACTCACTGAGCCACGTCCTCTGCTGTGACAAGTCCCCAGTTGCTTGGAAGAACATAGTGCCAACGAAGA
    AGGCCTGTCAGATACATCAACCGTGATCTGATGGGGATGGTAGCTCCAACTCCCATGTTTCCCTGCAACA
    GAGGGACAGACTAGCAACATTAACCAGCACCACAGGGATGCACCCAGCAAAGTGCAGATGGTGGGAAACC
    TAGTCGTTTCTTTAACACATACAGTACAAAGAAGGGAGGGCAGGATATATAACCCACAGGACTAGAAGAG
    AGGGAGAGAAGGGCCAAGAGGTGGGAAGAGGAGGAAAGAAAAGGAAAGGGAAAAAGACTTTTATGGGATA
    TGGTGGGAAATATGAACCTTGAGTAGTATTTGATTTTAGTGAGTTACTGTTAAATTCTTGACCAGGGTTG
    GGGATGCTGTGAAGAGATGGCTCAGTGGTTAAGAGCACTGGCTGCTCTTCAAGAGAACCTGGGTTCAATT
    CCCAGCCCCCACATGGAGGATCACACCTGTGTGTAACTCTTGTTCCAGAAGATCTGATGCCCTCACACAG
    GCATAGATGCAGGCAGAACAGCAATGTACAGAAAAAAATTCTTGCCTAGGATTTTGTTGTTGCTGCTGCC
    TTTGTGATACTGAGGAGGGAACCCAGTACCTTGTACGTGGTAGAAAAGCAGTTGACCACTGAGCCACGCC
    CCCAGCCCCTCACTGGGGGATTCTAGGCAGGGGCTCTACCACTGAGCCACGCCCCCGGGCTCTACCACTG
    AACATATTGATCTATACTTTTGGTTCATGTTTAAATTGTAAAATATTCTTCACTTTCTAAGAGAAAGTGA
    ACCATTCTATAAAAGTTCATTAAACAAATGAGAAAAAAACTACAGTGGCGGACAGGAAGTGTAGGTAGGA
    TGAAATTAGCCACGAGTCAGCAACTGTTGCACTTAACAATTGTGGGGATGTGAATTAATTAAGAAACCAT
    AATTCAGAAGTCCAAAAATCTCTCTGGCCATGATGAGGAACAGGAACAGAAGGAGCTCAAAGTCATTGGT
    AGGTATGCAGTGTGTTTGTGGCAACCTGGACTACATTAGACCCTGCCTCAAGAAAAGGGTTGGGGCACCA
    GAGCTCAGCTGCTCTTTTATACAGGACGCAGCAGCTCTGGGGCGTTCTAGGCACCCACATGGCTGCTCAC
    AACAATCCATTAAGGCCAGTTCCAGGGAATCTCATTCCTTCTTCTGGCCTCTGCGGGCACTGCACGGATA
    TGGCGTGTGCACACATATGTGCAGAGACATACTCCTACACATAAAACTTTCTTTTAAATTGCCAGGTGCG
    GTGGCGCATGTCTCTAATCCCAGCATTCAGGAAGCAGAGAGAGGCAGATCTCTGTTTGAGTTTGAGGCTG
    GCAAGTTCACTCACATAGCAAATTCCAGGACATAACATGTCGAGATCTTGTCTCAAGAACTAGGAGGAAT
    AATAAAAATAAAATTGGGGGCTGGGGATTTAGCTCAGTGGTAGAGCGCTTACCTAGGAAGCGCAAGGCCC
    TGGGTTCGGTCCCCAGCTCCGAAAAAAAAAAAACCAAAAAAAAAACAAAAAAAAAATGTGGAGCAGGAGG
    GTACTTGACATTTTATAAAGTCTGTGTAAATATCCCCTTATCCCCAAAAGCTTTTAGTCCCTAGCACTTC
    TGAAAAGGGGTATTCAGTTTGTATTGTAACACTCTGCTTCTGTGTCCGTTCGCAACTTTCCACGGCAAAG
    CTGGTTTTATGCCAGCTTATCGGCTCAAAAACCACCTCCCCCAGGGCCGCAGAGAGGGCCCTTCACAGAT
    TAGGCACGGCTGGCTCTCAGTAGACTGGCAAGGGAGGCTTGAAAAAGCAGTGGCTGTGAAAATCCTAAGA
    CTGCTTCCCAGAAAGAAAGGCCGATATGTGCCAGGCTGTGGTGGTGCACAGCTTTAATCCCAGCACTCAG
    TAGGCAGAGGCAGGTGGATCTCTGTGAGGGCAAGTCCAGCCTGGCCTGTAGATTGAGTTTCAGGACAGCT
    GGGGTTACAGAGAAGAACCTTGTCTCCAAAAAAAGAAAGCAAGCGAGCCCTGGGGTGTGTCACCCTGGGG
    TGTGTCACCCTGGGGTATATCACGGTTACCCGGAAGGCTTGCTTGTTCAATATGGGCCCAGTGTCTCTGT
    CTCAGCAGAAACGAGTGGGTAGAGTCCACAGGAGCCCCAGTACCCTGAGCCTGGAGCTCTGTGCTCTGAA
    AAGGAGGTGTCACCCTGTCCCGGGAGCTGCTCACGAGAACCTCCCTTTCAGCCACCCTACCCTGTTGTGT
    CTCCTTCCAGAGTTTCTTTCTCTCTGTGGCATACCTTCAGATGCTTTGTATGTCATTGTTGAAAACAGGG
    TCTCCCCGTGTAGCCCTGCCTGGATTAAAATGTTCTGCATGGACCAGGCTGGCTTTGAACTTGCAGCGAC
    CCTCCCATTTCTGCTTCCTGACTGCTGGGGTTATAGGTGTGCACGTGTGCATAATCATGCCTGCCTGGCT
    CACATGTGTCAAATTTTATTTATTATTTTATTTTACATGCATGTTTTGCCCACATGGGTATATGTGCACT
    GTGCATACGTGATACCCAGAGGACAAAAGAGGACATTGGATCCCCTAGGTCTGGAGTTACAGATGGCCAT
    GAGCCTCCATGGGGGTGCTGGGAATTGAACCTGGGTCTTCTGCAAGAGCAGCCACTGCACTTGACCACTG
    AGCCATCTCCTAGTCCCTTGCCCACATTTTTAGAGTTATTTTTAAAGGTGTAATGAGGAGGAACCTGCAT
    TTTCCTTCCTTGAATTACTTCGTTCTGATTCTAAAAATAATCCCAGCCCCTGCTAAGAGTGTGGGAAATC
    CAGAAAGCCATCACACCAGGGAACATAAAGACCCACGGGCAGCATCTTTAGTGCGCAGTCTGCCGGCCTC
    TTTGTAATGGGATTTGATTTACTCACTACCTGCATCTTTAGATCCTGATTCTGATACATGCCGTGAACAC
    AGGTGCCCAAGTGTTCTTCTGGTCAGCCTGGGCTACAAGGTGAATTCTGGGGCAACTTAGGGTACATGGC
    GAGACCTCATCTTAACTTTCCTCCCTCTTGAGGGGATCGTGCAGGGTGAGGTGGCAATTGCTGGTAATTC
    CAGCACTTGGGAGGCGAAGGCAGGAATTATCAGGAGTTCATGGCCAGCCTGGGTTACCTAACAAAACCCA
    GTCTTGAAACAAAGGACTGACCAGTACTCAGGAAGCAGGGACAGGAGGGCCTCGGGCAGCCTGGTCTTTG
    TAGTGAATACTGGGACAGCCAGGACTCCACAGAGAGAGTCTCTCTTAAAAAAAAAAAATGTAATGTTTGT
    CAGTTAATTCATATAACCAGCATTTGCCAAGCATGTGTACATTAAGAGACACTGCCCCATGGTGAGATGA
    ATCAGATGATATTTAATAAAAACAAGCGGAAACATTCTGAGCAGGAAAATAAAGCTGGTGAAGGGGGCAG
    GTTAGAAGCCAGGCCAGGAAAAATGAATAATAGTGGAAGCCAAGTGTGGTGCACCCTTGTGATCCCAGCA
    CTTGAGAGGCAGAGGCAGGAGGGTCAGGAGTTTAGGGTTGGCCTTGGCTACAAAGGGTCTTGGAGTCCAG
    CCTTGGCTACATGAAAGCCTGTTTCAGAAAAGACAAAGAGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC
    CTTGCCTAGGAAGCGCAAGGCCCTGGGTTCAGTCCCCAGCTCTGAGAAAAAAAAAAAAAAGAACCAGAAA
    AGACAAAGAACAAAAGACATCTTGGGGACTGATTAATTTTTCTATTCTGATGAAAATCATTTTATGGTGG
    AAAATTTAAATATAGACAGAAAAAGCAGAGGTAGTATAACCTTCCAGCTTCCAGAATTATCAGGATTTTG
    ATGACTCGCTTCAGACAGTTTGGGGCTGGAGACTTGGCCCAAGAGTTAAAAGCACTGGCTGTTCTTCCAG
    AGGACCTGGGTTCAGTTCCAAGCACCCGTATATGGTCGCTTAAAAGGACAACTATCAATAACTCCAGTTT
    CCGGGAACCTGATGCCCTCTTCTGGCCTTCTTGGGCATTGCACAAATATGGTGCACATAAAAATAAATCT
    TTAAAACAGAACAAGCCAGGTGGTGGTGGTGCACACCTTTAGTCCCAGCACTCTGGAGGCAGAGACAGGT
    GCGTTCCCAAGTTTGAGGCCAGCCTGGTCTACAGAGTGAGTTCTGGGTCAGCCAGGGCTACACAGAGAAA
    CTCTGTCTCAAGCAAGCAAACAAACAATACCGAAACAGCTGCATAGAAATTCACAGTCCTTGCAGTAAGT
    GTAGCATTGAATTCCCAGGATGGAATTGAACAGTTGTATGGAGGGACGCTTTGGATGGCAAGTGGGCAGG
    GCTGGTCAGAGGAGGGAGGCACTGTTCATTTATGTAACGGGGATACTGAATATCTGCCTCAAGCCAGGCA
    GCATTTTTAGGAGTTAAAGCTCCCTGGGTGGTAGATAGTATTTAAAGGCACCGTCTTACCCTGTAGCCGT
    AGCTACCCTGGAACCGTCAGGGATCCAGCCTACCCTGCCTCTTTGTTGGTAGTAAAGGTATGCACCGCCA
    TGCACGGATTCACTTTAATCTTGTTGTTATTTTGTGCAAGTGCCCACACATGCTGTGGTGCACACGAGCA
    GGGCAGAGGTCAGTCTCGTGGAGCCTCTTCTCCACACTCAACTCATCCGGTTTTCAAGTCAGGCTCCTTT
    ATCCGCTAGGCTGTCTTGTCAACCCTGAGTCAAACCTAAATGTAGTGAAGGAGGAAGCCAGAGTGGGCAT
    CTCTGGGAAGAACAAGTCAGGCAAACAAAAACAACCAGCGTGTGTAAATGCCCTGAGGTAGGCTTGACTG
    TGTCTGAGGACTAGAGAGGAAGCCTGTGTGGCTGATGTCAGGGGTGGGTGAGGGCAGAAGCTGAGGAAAG
    CCATATTCGGTGTACCTGTGACTCATGGTGATAATTTAGGCATTTGTAGTAAAGGGGAAGGAAGAGTATC
    ATAGCCGGCTATGGCCTCCAAAGGCTGCCCTCGGCCTTGTTTGTGCCACTCAGCTGCACAGGCCTTAGCC
    CCACATGCAGTAGAGGTTCAGTAAACACTTGTCGGACAGATAGGATGTGTCGGCACACGTGGGGCCGTGG
    ACATGGCATGATTTATTACGTCCCCCCCGTGAGTGCTCATGGGCCTTCTCAGTGCCTGTCGTGATGGATG
    GTTGTAGTGTTTCTGTTATCGCAGGGGTCCTGGGGAGCCTGGGCTGGAGGCTGAGGGCAGCCTAGGGTTG
    GAGGGGGCAGCAGGGTTGTCCCTGCTCCTGACCTTTGTTCCTCCCGCCTGCAGCTCCACAGAGAAGAACT
    GCTGTGTACGGCAGCTGTACATTGACTTTAGGAAGGACCTGGGTTGGAAGTGGATCCACGAGCCCAAGGG
    CTACCATGCCAACTTCTGTCTGGGGCCCTGCCCCTACATTTGGAGCCTGGACACACAGTACAGCAAGGTG
    GGTCTGGCCTGTGGGACAGGCTGGAGCAGAGGCTGGGTGGTTGGGAGAGAATGAGAGGCTAGAGCAGTGG
    GGGAGTCAAGGGAATAGGGTGACAGGGAAACAGAGGAGAGACAGAAATACAGAGAGGCAGAGTTGGAGAG
    ATGGGAAGAAACAGATTAGGTGAGAAAGACAGGAGAGCTAGTGATGTGGAGAGACACAGAAATGGAGGTG
    GGGAGGATTGGAGATGGGAACTAGAGTAAGTGACCAAGACAGACAGGGAGGGGAGATAAGATGAAGAGAG
    GGAGAGAGGAAGAGGCAGAGATGGGGGCAGATGAGAGAGAGGTGGAACATTAGGAAGAGACATAAATAGA
    AATAGACTAGGATAGGTATCGAGGCCGATGGAAGCTGGGGAGACTCAGAGATAAGAGACAGAAATGTAGA
    GATCCTGGTAGGGAGAGAGACCGGGGAGTCCCTGGACACTGGCAGTGACTCTGGCCCACCCGCAGGTCCT
    TGCCCTCTACAACCAACACAACCCGGGTGCTTCCGCATCACCGTGCTGCGTGCCGCAGGCTTTGGAGCCA
    CTGCCCATCGTCTACTACGTGGGTCGCAAGCCCAAGGTGGAGCAGTTGTCCAACATGATCGTGCGCTCCT
    GCAAGTGCAGCTGAGGCCCCGCCCCCGCCCCGCCCCGCCCCTCCCGGCAGGCCCGGCCCCGCCCCCGCCC
    CGCCCGCAGTGCCCGGGGCTGTATTTAAGGACACCTGCACCCCCCCCAAGCCCATCTGGGGGCCCATTAA
    AGGTGACAGAGG
  • Protein Sequence : Show Sequence
    >gi|11024652|ref|NP_067589.1| transforming growth factor beta-1 precursor [Rattus norvegicus]
    MPPSGLRLLPLLLPLPWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPP
    GPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYDKTKDITHSIYMFFNTSDI
    REAVPEPPLLSRAELRLQRFKSTVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQ
    GDGIQGFRFSAHCSCDSKDNVLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRAL
    DTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGA
    SASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS
  • Molecule Role : Other
  • Related Vaccine(s): Adjuvant arthritis DNA vaccine pcDNA-CCOL2A1 encoding type II collagen
5. TNF-alpha
  • Gene Name : TNF-alpha
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 21926
  • NCBI Protein GI : 7305585
  • Genbank Accession : AB039224
  • Protein Accession : NP_038721
  • Taxonomy ID : 10090
  • Chromosome No : 17
  • Gene Starting Position : 35199389
  • Gene Ending Position : 35201995
  • Gene Strand (Orientation) : -
  • Protein Name : tumor necrosis factor
  • Protein pI : 4.75
  • Protein Weight : 24010.47
  • Protein Length : 235
  • Protein Note : Also known as DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434
  • DNA Sequence : Show Sequence
    >gi|372099093:35199389-35201995 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J
    CTTTATTTCTCTCAATGACCCGTAGGGCGATTACAGTCACGGCTCCCGTGGGGAGCAGAGGTTCAGTGAT
    GTAGCGACAGCCTGGTCACCAAATCAGCGTTATTAAGACAATTGGGTTAGATAAATATTTTGTTTTAAAC
    ATAAGCAAAAGAGGAGGCAACAAGGTAGAGAGGCCAGGTGGGGACAGCTCAGCTCCGTTTTCACAGAAAA
    CATGTCTGTCTGAAGACAGCTTCCCACACTGGGTCCTCCAGGACACCCCGGCCTTCCAAATAAATACATT
    CATAAGCAAATAAATAAATAATAAATAAATAATAAATAATAAGTGCAAATATAAATAGAGGGGGGCTGGC
    TCTGTGAGGAAGGCTGTGCATTGCACCTCAGGGAAGAATCTGGAAAGGTCTGAAGGTAGGAAGGCCTGAG
    ATCTTATCCAGCCTCATTCTGAGACAGAGGCAACCTGACCACTCTCCCTTTGCAGAACTCAGGAATGGAC
    ATTCGAGGCTCCAGTGAATTCGGAAAGCCCATTTGAGTCCTTGATGGTGGTGCATGAGAGGCCCACAGTC
    CAGGTCACTGTCCCAGCATCTTGTGTTTCTGAGTAGTTGTTGAAAGCTCTGAGCACAGAGTTGGACCCTG
    AGCCATAATCCCCTTTCTAAGTTAGAAGGATACAGACTGGGGGCTCTGAGGAGTAGACAATAAAGGGGTC
    AGAGTAAAGGGGTCAGAGTGGGGGCTGGGTAGAGAATGGATGAACACCCATTCCCTTCACAGAGCAATGA
    CTCCAAAGTAGACCTGCCCGGACTCCGCAAAGTCTAAGTACTTGGGCAGATTGACCTCAGCGCTGAGTTG
    GTCCCCCTTCTCCAGCTGGAAGACTCCTCCCAGGTATATGGGCTCATACCAGGGTTTGAGCTCAGCCCCC
    TCAGGGGTGTCCTTGGGGCAGGGGCTCTTGACGGCAGAGAGGAGGTTGACTTTCTCCTGGTATGAGATAG
    CAAATCGGCTGACGGTGTGGGTGAGGAGCACGTAGTCGGGGCAGCCTTGTCCCTTGAAGAGAACCTGGGA
    GTAGACAAGGTACAACCCATCGGCTGGCACCACTAGTTGGTTGTCTTTGAGATCCATGCCGTTGGCCAGG
    AGGGCGTTGGCGCGCTGGCTCAGCCACTCCAGCTGCTCCTCCACTTGGTGGTTTGCTGAGGGGGGGGGGG
    AGGATTGAGTCAGTGTCACCCTCTTAGTTCACACTCCACATCCTGAGCCTCAGCAGCTACCCACACTTCA
    CTTCCGGTTCCTGCACCCTCTGTCTTTCCACATCCCATTGGCTATGAGGTCCCGGGTGGCCCCCTGATGC
    CTTGCTTTTGAGTCACTGCTCTGACTCTCACGTGCTGTCTCTAAGAGCTCTGTCTTTTCTCAGCCTGGCT
    CGACACCCCTCAACCCGCCCCCCAAAATCATGCCCCTTCATTCTCAAGGCACATGTAAAGAAATCTTACC
    TACGACGTGGGCTACAGGCTTGTCACTCGAATTTTGAGAAGATGATCCTGGAGGGGAAGAGACAAAGGCA
    AGGATGAGCCTTTTAGGCTTCCCAGCAAGCATCTATGCACTTAGACCCCTTTCCTCCCAAACCAAAGCTT
    TAAGTTCTCCCCCCACCCCATCTCATCCCATGCCTAACTGCCCTTCCTCCATCTTAAATTAAGAGAGAGG
    TGTGGGAACACTTACTGAGTGTGAGGGTCTGGGCCATAGAACTGATGAGAGGGAGGCCATTTGGGAACTT
    CTGTGTAGGAAAAGGAGGTTAGTTAAGACAGACTCACCCCAAAGGAGAAGCCTCCCGGCTGATTGCCCCG
    CTTACAGTTCCTCTTTGCCCCACCCCACCCCCCAGCTTTGTGTTTTTCTTCTTCATTCATTCATCTGTCC
    AACCCACGGCTTCTTTCTGCGGTGCCCTCTGTGCTTGATCTCCCGTTATCTCCCCTTCATCTTCCTCCTT
    ATCTCTCATGCCTCTCTCATTTCTGTCTCTGAGTTTTATCTCTTGCTTATCCCCTCTTCCCCTGGCCACA
    TCTTTCCAGATCTCTCCACGTGTGAACACACTTGTTCGTTCATTCATCTCTCTGTGCATCCGACGAAGGA
    TGTTTAGTCAGCTGGACGCATGGGTCCGAGGTCCTGACTCTGTCCCCTCCACACTCTCCTCCACCTTGCC
    CTGCCCATTAGCCCACTTCTTTCCCTCACACTGTCCTTCTTGCCCTCCTAACCCGTTTTGCTTGTGAGCG
    AGAATAAGGGTTGCCCAGACACTCACCTCATCCCTTTGGGGACCGATCACCCCGAAGTTCAGTAGACAGA
    AGAGCGTGGTGGCCCCTGCCACAAGCAGGAATGAGAAGAGGCTGAGACATAGGCACCGCCTGGAGTTCTG
    GAAGCCCCCCATCTTTTGGGGGAGTGCCTCTTCTGCCAGTTCCACGTCGCGGATCATGCTTTCTGTGCTC
    ATGGTGTCTTTTCTGGAGGGAGATGTGGCGCCTTGGGCCAGTGAGTGAAAGGGACAGAACCTGCCTGGTT
    GGCTGCTTGCTTTTCTGGGAGCTATTTCCAAGATGTTCTGGAGTTTCTGTTCTCCCTCCTGGCTAGTCCC
    TTGCTGTCCTCGCTGAG
  • Protein Sequence : Show Sequence
    >gi|7305585|ref|NP_038721.1| tumor necrosis factor [Mus musculus]
    MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRDEKFPNGLPL
    ISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQ
    VLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQL
    SAEVNLPKYLDFAESGQVYFGVIAL
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Adjuvant arthritis DNA vaccine CII encoding type II collagen
6. TNF-alpha from Rattus norvegicus
  • Gene Name : TNF-alpha from Rattus norvegicus
  • Sequence Strain (Species/Organism) : Rattus norvegicus
  • NCBI Gene ID : 24835
  • NCBI Protein GI : 6981662
  • Genbank Accession : AABR06099883
  • Protein Accession : NP_036807
  • Taxonomy ID : 10116
  • Chromosome No : 20
  • Gene Starting Position : 6936228
  • Gene Ending Position : 6938846
  • Gene Strand (Orientation) : +
  • Protein Name : tumor necrosis factor
  • Protein pI : 4.9
  • Protein Weight : 24217.89
  • Protein Length : 235
  • Protein Note : Also known as Tnfa; RATTNF; TNF-alpha
  • DNA Sequence : Show Sequence
    >gi|389675109:6936228-6938846 Rattus norvegicus strain BN/SsNHsdMCW chromosome 20, Rnor_5.0
    ACACCAAGGGACCAGCCAGGAGGGAGAACAGCAACTCCAGAACACCCTGGTACTAACTCCCAGAAAAGCA
    AGCAACCAGCCAGGCAGGTTCCGTCCCTCTCATACACTGGCCCGAGGCAACACATCTCCCTCCGGAAAGG
    ACACCATGAGCACGGAAAGCATGATCCGAGATGTGGAACTGGCAGAGGAGGCGCTCCCCAAAAAGATGGG
    GGGCCTCCAGAACTCCAGGCGGTGTCTGTGCCTCAGCCTCTTCTCATTCCTGCTCGTGGCGGGGGCCACC
    ACGCTCTTCTGTCTACTGAACTTCGGGGTGATCGGTCCCAACAAGGAGGAGGTGAGTGCCTGGGCAGCGT
    TTATTCTCACTCACAAGCAAAGTAGGGTGGGAGGGCAAGAAAGGCAGGAGGGTAAGGGAAAGAGGTCGGC
    TGGTGGGCACAGTAATGTGGAGGAGAGTTGGGGAGGGAGACCTCGGACCCGTGCAGCCAGTTGTCTAAAC
    ATCCTTGGGTGGATGCACGGAGGGGCGAATGAACGAACAAGCGTGCGTACACGTGCAGAGATGTGCAGAG
    ACGTGGCCAGGGGAACAGGGAAGAAGCAAGAGATAAAAATTCGAGACGGAGATGGGAGACGAGGGAGATA
    AGGAGATATGAGAGATAAGGAGGGAGATGGAGGGGAAACAACAGATCAAGCACAGAGGGTACCACAGAAA
    GATGCTGAGGTTGGACGGATAAATGAATGAGGAGGAAAAGCACTGACTGGGGGGGGGGGGCTGGCAAAGA
    GCGGGGAGGCTTCTCCTTTGTGGTGAGTCTGTCTACTAACCTACTTCTTTCCCTACACAGAAGTTCCCAA
    ATGGGCTCCCTCTCATCAGTTCCATGGCCCAGACCCTCACACTCAGTAAGTGTTCCCAAGCCTCTCTCTT
    AATTTAGGATGGGGGGCGGGGGACTTAAAGCTTTGGTTTGGGAGGAAAGGGAATAGGGGTCTAACTACAC
    AGATGCTTGCTGGGAAACCTAAGGGTCTCATCTCCGCCTTTGTCCCCTTCCAGGATCATCTTCTCAAAAC
    TCGAGTGACAAGCCCGTAGCCCACGTCGTAGGTAAGTTTCTTCGCATGTGCCTTGGGGGGGGGGGTGTGA
    CAGGGCAGGATTTTGGGGTCGTGCCAGGCTGAGAGAAGACAAGGGCTCTCAGAGACAGCAGGTGAGAGAG
    TCAGAGCGGTGATTCAAAGGCAAGGCATTAGGAGGCCACCCCGGACCTCGTAGCCAATGGGACGTGGAAA
    GACCAGAGGGCGCAGGAACCGGAAGTGAAGTGTGGGTAGAAGTTGAGGTTCAGGATGTGGAGTGTGAACT
    AAGAGGGTGACACTGACTCAATTCTCCCTCCCTCAGCAAACCACCAAGCGGAGGAGCAGCTGGAGTGGCT
    GAGCCAGCGTGCCAACGCCCTCCTGGCCAATGGCATGGATCTCAAAGACAACCAACTGGTGGTACCAGCA
    GATGGGCTGTACCTTATCTACTCCCAGGTTCTCTTCAAGGGACAAGGCTGCCCCGACTACGTGCTCCTCA
    CCCACACCGTCAGCCGATTTGCCATTTCATACCAGGAGAAAGTCAGCCTCCTCTCTGCCATCAAGAGCCC
    TTGCCCTAAGGACACCCCTGAGGGAGCTGAGCTCAAGCCCTGGTATGAGCCCATGTACCTGGGAGGAGTC
    TTCCAGCTGGAGAAGGGGGACCTGCTCAGCGCTGAGGTCAACCTGCCCAAGTACTTAGACATCACGGAGT
    CCGGGCAGGTCTACTTTGGAGTCATTGCTCTGTGAGGCGACTGGCGTGTTCATCCGTTCTCTACCCAGCC
    CCTGTCCCCGACTCTGACCCCCATTACTCTGACCCCTTTATCGTCTACTCCTCAGAGCCCCCAATCTGTG
    TCCTTCTAACTTAGAAAGGGAATTGTGGCTCTGGGTCCAACTCCGGGCTCAGAATTTCCAACAACTACGA
    TGCTCAGAAACACACGAGACGCTGAAGTAGTGGCCTGGATTGCGGGCTGCTCATGAGCCGCCACCGGCAA
    GGATTCCAATGGGCTTTCGGAACTCACTGGATCCCGGAATGTCGATGCCTGAGTGGATCGGGGGAGTGGA
    GGGGGGAGTGGATCAGGCTGCCTCTGGCTCACAAGGCTGCTGAAGATGTCTCAGGCCTCCCTTCCTTCCT
    TCCTTCCCTCCCTCAGAGCTTCCCAGACTGTTCCCTGAGGCACAGCGCCCAGCCTTCCTTACGGAACCCC
    CTCTATTTATAATTGCACCTGTGACTATTTATTTATTATTTATTATTTATTTATTTGCTTATGAATGTAT
    TTATTTGGAAGGCCGGGGCGTCCTGGAGGACCCAGCGTTGGGAAGCTGTCTTCAGGCCAACATGTTTTCT
    GTGAAAACGGAGCTAAACTACCAGCTATCCCCATCTGGTCCCCCACCCCTGCCTCGTTGCCTCCCCCCTT
    TTCTTTTGCTTATGTTTAAAACAAGATATTTATCTAACCCAGTTGTCTTAATAATGCTGATTTGGTGACC
    AGGCTGTCGCTACATCACTGAACCTCTGCTCCCCACGGGAGCCGTGACTGTAATCGCCCTACGGGTCATT
    GAGAGAAATAAAGTTCGCTTGGAAAAGAA
  • Protein Sequence : Show Sequence
    >gi|6981662|ref|NP_036807.1| tumor necrosis factor [Rattus norvegicus]
    MSTESMIRDVELAEEALPKKMGGLQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPNKEEKFPNGLPL
    ISSMAQTLTLRSSSQNSSDKPVAHVVANHQAEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLIYSQ
    VLFKGQGCPDYVLLTHTVSRFAISYQEKVSLLSAIKSPCPKDTPEGAELKPWYEPMYLGGVFQLEKGDLL
    SAEVNLPKYLDITESGQVYFGVIAL
  • Molecule Role : Other
  • Related Vaccine(s): Adjuvant arthritis DNA vaccine pcDNA-CCOL2A1 encoding type II collagen
IV. Vaccine Information
1. Adjuvant arthritis DNA vaccine CII encoding type II collagen
a. Vaccine Ontology ID:
VO_0004409
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of Collagen type II
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
f. Immunization Route
Intramuscular injection (i.m.)
g. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: In vitro analysis of anti-CII T cells demonstrated reduced production of the pro-inflammatory cytokines IFN-γ and TNF-α in CII vaccinated mice. Further, CII DNA vaccines reduced epitope spreading of auto-reactive B cell responses in CIA (Ho et al., 2006).
  • Efficacy: DNA encoding CII reduced the disease severity and disease incidence of collagen-induced arthritis to 50% as compared to the PBS-treated control group in which the disease was more severe and the disease incidence was 89% (Ho et al., 2006).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: CII-stimulated lymphocytes isolated from CIA mice treated with a DNA vaccine encoding CII produced significantly less IFN-g and TNF-a compared to PBS and DNA vector-IL-4 treated mice (Ho et al., 2006).
    • Detailed Gene Information: Click here.
  • Host Gene Response of TNF-alpha
    • Gene Response: CII-stimulated lymphocytes isolated from CIA mice treated with a DNA vaccine encoding CII produced significantly less IFN-g and TNF-a compared to PBS and DNA vector-IL-4 treated mice (Ho et al., 2006).
    • Detailed Gene Information: Click here.
2. Adjuvant arthritis DNA vaccine pcDNA-CCOL2A1 encoding type II collagen
a. Vaccine Ontology ID:
VO_0004410
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Rat
e. Gene Engineering of Collagen type II
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3 expressed chicken type II collagen (CCOL2A1) (Song et al., 2009).
  • Detailed Gene Information: Click here.
f. Vector:
pcDNA3 (Song et al., 2009)
g. Immunization Route
Intramuscular injection (i.m.)
h. Rat Response
  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: CD4+CD25+Treg cells play a role in restraining auto-aggressive T cells in experimental settings, and 200 μg/kg of pcDNA-CCOL2A1vaccine increased the proportion of CD4+CD25+Treg cells in the blood of arthritic rats compared with those in all other groups (Song et al., 2009).
  • Efficacy: Comparison of all treated and control groups demonstrated clearly that 200 μg/kg of pcDNA-CCOL2A1 vaccine alone in a single intravenous treatment significantly reduced the severity of disease, footpad swelling, arthritic incidence, clinical scores, and deferred the onset of disease (Song et al., 2009).
  • Host Gene Response of IgG (partial)
    • Gene Response: pcDNA-CCOL2A1 vaccine significantly reduced the levels of serum anti-CII IgG antibodies compared to the negative control group (P < 0.05) (Song et al., 2009).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Il10 from Rattus norvegicus
    • Gene Response: Concentrations of the Th2-cytokine IL-10 in 200 μg/kg of pcDNA-CCOL2A1 vaccine group were significantly higher than those in all other groups, including the MTX positive control group (P < 0.05) (Song et al., 2009).
    • Detailed Gene Information: Click here.
  • Host Gene Response of TGF-B from Rattus norvegicus
    • Gene Response: Concentrations of the Th3-cytokine TGF-β in 200 μg/kg of pcDNA-CCOL2A1 vaccine group were significantly higher than those in all other groups, including the MTX positive control group (P < 0.05) (Song et al., 2009).
    • Detailed Gene Information: Click here.
  • Host Gene Response of TNF-alpha from Rattus norvegicus
    • Gene Response: Concentrations of the Th1-cytokine TNF-α in both 200 μg/kg of pcDNA-CCOL2A1 vaccine and MTX groups were significantly lower than those in the negative control and other treatment groups (P < 0.05). Of note, the level of TNF-α in 200 μg/kg of pcDNA-CCOL2A1 vaccine group recovered to that of the normal group (Song et al., 2009).
    • Detailed Gene Information: Click here.
3. Adjuvant arthritis DNA vaccine pCMV3.65 encoding HSP65
a. Vaccine Ontology ID:
VO_0004407
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Rat
e. Gene Engineering of hsp65 from M. leprae
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3 expressed mycobacterial heat shock protein 65 (hsp65) (Ragno et al., 1997).
  • Detailed Gene Information: Click here.
f. Vector:
pcDNA3 (Ragno et al., 1997)
g. Immunization Route
Intramuscular injection (i.m.)
h. Rat Response
  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: Rats treated with pCMV3.65 showed a significantly elevated antibody levels (median 0.707) when compared with vector-treated rats (median 0.351; P < 0.01) and with naive animals (median 0.0715; P < 0.01). Similarly, spleen cells from pCMV3.65-treated rats exhibited significantly higher proliferative responses against the mycobacterial recombinant antigen when compared with vector treated animals (P < 0.05) or with naive rats (P < 0.01) (Ragno et al., 1997).
  • Efficacy: The pCMV3.65-treated rats were significantly protected from disease development in comparison with the control groups injected with the empty plasmid (Ragno et al., 1997).
4. Pristane-induced arthritis DNA vaccine DNA-hsp65 encoding Mycobacterium leprae 65-kDa heat shock protein
a. Vaccine Ontology ID:
VO_0004408
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of hsp65 from M. leprae
f. Vector:
pcDNA3 (Santos-Junior et al., 2005)
g. Immunization Route
Intramuscular injection (i.m.)
h. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: Cellular and humoral immune responses to the protein are elicited, and long-lived memory lymphocytes are induced (Santos-Junior et al., 2005).
  • Efficacy: DNA-hsp65 vaccination was protective against arthritis in AIRmax mice (maximal acute inflammatory reaction), as evidenced by the absence of arthritis incidence during the 150-day follow-up period. This protective effect was more pronounced after the onset of pristane-induced arthritis (Santos-Junior et al., 2005).
V. References
1. Ho et al., 2006: Ho PP, Higgins JP, Kidd BA, Tomooka B, Digennaro C, Lee LY, de Vegvar HE, Steinman L, Robinson WH. Tolerizing DNA vaccines for autoimmune arthritis. Autoimmunity. 2006; 39(8); 675-682. [PubMed: 17178564].
2. Mayo Clinic - Arthritis: Arthritis [http://www.mayoclinic.com/health/arthritis/DS01122]
3. Quintana et al., 2002: Quintana FJ, Carmi P, Mor F, Cohen IR. Inhibition of adjuvant arthritis by a DNA vaccine encoding human heat shock protein 60. Journal of immunology (Baltimore, Md. : 1950). 2002; 169(6); 3422-3428. [PubMed: 12218165].
4. Ragno et al., 1997: Ragno S, Colston MJ, Lowrie DB, Winrow VR, Blake DR, Tascon R. Protection of rats from adjuvant arthritis by immunization with naked DNA encoding for mycobacterial heat shock protein 65. Arthritis and rheumatism. 1997; 40(2); 277-283. [PubMed: 9041939].
5. Santos-Junior et al., 2005: Santos-Junior RR, Sartori A, De Franco M, Filho OG, Coelho-Castelo AA, Bonato VL, Cabrera WH, Ibañez OM, Silva CL. Immunomodulation and protection induced by DNA-hsp65 vaccination in an animal model of arthritis. Human gene therapy. 2005; 16(11); 1338-1345. [PubMed: 16259568].
6. Song et al., 2009: Song X, Liang F, Liu N, Luo Y, Xue H, Yuan F, Tan L, Sun Y, Xi C, Xi Y. Construction and characterization of a novel DNA vaccine that is potent antigen-specific tolerizing therapy for experimental arthritis by increasing CD4+CD25+Treg cells and inducing Th1 to Th2 shift in both cells and cytokines. Vaccine. 2009; 27(5); 690-700. [PubMed: 19095031].