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Mycobacterium tuberculosis

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Disease
    3. Introduction
    4. Microbial Pathogenesis
    5. Host Ranges and Animal Models
    6. Host Protective Immunity
  2. Vaccine Related Pathogen Genes
    1. SodA
    2. Ag85A (Protective antigen)
    3. Ag85B from M. tuberculosis H37Rv (Protective antigen)
    4. Apa (Protective antigen)
    5. bfrA (Protective antigen)
    6. DnaK (Protective antigen)
    7. ESAT-6 (Protective antigen)
    8. EsxA (ESAT-6) (Protective antigen)
    9. EsxB (Protective antigen)
    10. FbpA (Ag85A) (Protective antigen)
    11. FbpB from M. tuberculosis (Protective antigen)
    12. FbpD (Protective antigen)
    13. HBHA (Protective antigen)
    14. KatG (Protective antigen)
    15. Mpt63 (Protective antigen)
    16. MPT64 (Protective antigen)
    17. MPT83 (Protective antigen)
    18. Mtb72F (Protective antigen)
    19. PE20 (Protective antigen)
    20. PepA (Protective antigen)
    21. PPE14 (Protective antigen)
    22. PPE18 (Protective antigen)
    23. PPE31 (Protective antigen)
    24. PstS1 (Protective antigen)
    25. PstS3 (Protective antigen)
    26. Rv1806 (Protective antigen)
    27. Rv1807 (Protective antigen)
    28. Rv2660c (Protective antigen)
    29. drrC (Virmugen)
    30. fadD26 (Virmugen)
    31. leuD (Virmugen)
    32. lysA (Virmugen)
    33. mce-2 (Virmugen)
    34. mce-3 (Virmugen)
    35. panC (Virmugen)
    36. panD (Virmugen)
    37. PhoP (Virmugen)
    38. proC (Virmugen)
    39. RD1 (Virmugen)
    40. SecA2 (Virmugen)
    41. sigE (Virmugen)
    42. sigF (Virmugen)
    43. trpD (Virmugen)
  3. Vaccine Related Host Genes
    1. APOBEC3B
    2. C19orf66
    3. CD70
    4. Cxcl10
    5. Cxcl11
    6. Cxcl9
    7. Defb3
    8. Defb4
    9. Ebi3
    10. Fth1
    11. Ftl
    12. GPR182
    13. GPX1
    14. HIST1H2BE
    15. HIST2H4A
    16. human IFNG
    17. IFNG
    18. Ifng (Interferon gamma)
    19. IL-1b
    20. IL-6
    21. IL10
    22. IL10
    23. Il10 (interleukin 10)
    24. Il12b
    25. Il15
    26. Il17f
    27. IL2
    28. Il2
    29. Il4 (interleukin 4)
    30. IL6
    31. lipY
    32. Ltf
    33. Nos2
    34. PCDHB1
    35. PTCD1
    36. SLC11A1
    37. STAG3
    38. TBX21
    39. TLR1
    40. TNF-alpha
    41. TSC22D4
  4. Vaccine Information
    1. Ag85B and ESAT-6 fusion protein
    2. Arabinomannan-tetanus toxoid conjugate
    3. attenuated M. bovis strain WAg539
    4. Bacille Calmette-Guérin (BCG)
    5. BCG auxotroph expressing HIV-1 clade immunogen
    6. BCG mutant secreting listeriolysin
    7. BCG Vaccine (Freeze-Dried)
    8. BCG-DHTM
    9. DNA vaccine co-expressing A85A and caspase-3
    10. DNA vaccine Rv1806-1807
    11. ΔureC hly+ rBCG
    12. LT-BCG- Ag85B /Rv3425
    13. M. bovis WAg520 strain
    14. M. bovis WAg522 strain
    15. M. tuberculosis DNA vaccine (containing the ESAT-6, MPT-64, MPT-83, and KatG constructs)
    16. M. tuberculosis DNA Vaccine APADNA priming and APAMVA boosting
    17. M. tuberculosis DNA vaccine DNAAg85A encoding a single immunogenic M.tb Ag
    18. M. tuberculosis DNA Vaccine encoding Ag85B Protein
    19. M. tuberculosis DNA vaccine encoding Ag85B, MPT64 and MPT83
    20. M. tuberculosis DNA Vaccine encoding KatG
    21. M. tuberculosis DNA vaccine ESAT-6
    22. M. tuberculosis DNA vaccine pAK4-sod
    23. M. tuberculosis DNA vaccine pcDNA3.1+/Ag85A DNA encoding Ag85A
    24. M. tuberculosis HBHA Protein Vaccine
    25. M. tuberculosis Mtb72F Protein Subunit Vaccine
    26. M. tuberculosis phoP mutant SO2
    27. M. tuberculosis secA2 mutant
    28. M.S-Δesx-3-IKEPLUS (M. tuberculosis)
    29. Mtb72f fusion protein
    30. MVA/IL-15/5Mtb vaccine
    31. Mycobacterium tuberculosis drrC mutant vaccine
    32. Mycobacterium tuberculosis fadD26 mutant vaccine
    33. Mycobacterium tuberculosis leuD mutant vaccine
    34. Mycobacterium tuberculosis lysA/secA2 mutant vaccine
    35. Mycobacterium tuberculosis mce-2 mutant vaccine
    36. Mycobacterium tuberculosis mce-3 mutant vaccine
    37. Mycobacterium tuberculosis panCD mutant vaccine
    38. Mycobacterium tuberculosis proC mutant vaccine
    39. Mycobacterium tuberculosis RD1/panCD mutant vaccine
    40. Mycobacterium tuberculosis sigE mutant vaccine
    41. Mycobacterium tuberculosis trpD mutant vaccine
    42. Mycobax
    43. rBCG-Ag85A/ Ag85B
    44. rBCG-Ag85A[Tokyo]
    45. Recombinant BCG, Ag85A in multiple vectors
    46. recombinant S. typhimurium secreting M. tuberculosis ESAT-6
    47. Two recombinant BCG strains expressing MSP
  5. References
I. General Information
1. NCBI Taxonomy ID:
1773
2. Disease:
Tuberculosis
3. Introduction
At the end of 19th century, Robert Koch discovered that Mycobacterium tuberculosis was the causative agent of tuberculosis (TB). TB is a major cause of morbidity and mortality throughout the world despite more than a half century of widespread vaccination, claiming millions of lives every year. The extraordinary high prevalence of the latent infection [estimated at one-third of the world's population] is one of the main factors contributing to the high incidence of active tuberculosis. The other factor is convergence of the TB and HIV epidemics. With HIV/AIDS fueling the TB crisis, the WHO has estimated that more than 1 billion people will be infected with TB bacilli in the next 20 years (Gupta et al., 2007).
M. tuberculosis are Gram-positive bacilli lacking both flagella and capsules. M. tuberculosis is preferentially established in the pulmonary system in a dormant state after initial infection via aerosol transmission. The M. tuberculosis complex, a group of Mycobacterium including M. tuberculosis, M. bovis, M. bovis BCG, M. africanum, and M. microti, are tuberculosis-inducing bacteria which have low horizontal gene transfer and variable levels of virulence (Ducati et al., 2006).
4. Microbial Pathogenesis
Mouse and guinea pig models have been used extensively to understand the host and pathogen-specific factors controlling pathogenesis of tuberculosis. Transmission of M. tuberculosis via intravenous route in murine models is followed by initial bacterial replication and subsequent macrophage activation via IL-6, IL-12, and TNF-mediated mechanisms. Bacterial load is significantly reduced at 2 weeks post-infection in liver and spleen. After load reduction, selected lymphocytes are activated which can then induce macrophages to begin producing nitric oxide via the iNOS pathway. Bacteria which survive the initial clearance by the host enter a viable yet non-replicating persistence stage. Factors such as immune suppression from aging, iNOS inhibition, and corticosteroid therapy can shift the dormant bacilli back into a replicative state. As an example of preferred host niche, transmission via inhalation will sometimes result in bacilli establishing infection in macrophage phagosomes in the lung. These macrophages become lodged in the calcified tubercles, and can later initiate formation of granulomas through a CD4-mediated delayed-type hypersensitivity reaction. Each granuloma can remain dormant, reduce in size and possibly disappear, initiate progressive interstitial fibrosis in lung tissue, and/or necrotize (Ernst et al., 2007). Similar mechanisms are observed in liver tissue (Ducati et al., 2006).
Numerous pathogen- and host-specific factors related to pathogenesis of tuberculosis are potential vaccine targets. For example, the Bacille-Calmette Guiren (BCG) vaccine was obtained from an M. bovine strain which lost its virulence after 39 passages (Ducati et al., 2006). Evidence from subtractive hybridization experiments, along with whole-genome microarrays and BAC arrays and other experimental evidence, suggests that the RD1 locus is the main virulence feature that is absent from 13 strains of avirulent BCG yet present in hundreds of other M. tuberculosis strains (Ernst et al., 2007). RD1 is related to the regulation of the early secreted antigen 6 kDa [ESAT-6] secretion system 1[ESX-1], an important pathway which is linked to the recruitment and infection of macrophages to initially infected sites and subsequent disease propagation (Ernst et al., 2007).
5. Host Ranges and Animal Models
Host ranges extend to many animals, including human, mouse, guinea pig, rabbit, and cattle. The most predominant animal used to study M. tuberculosis for virulence assessment and vaccine development is likely the mouse, which include C57BL/6 and BALB/c strains, mainly due to cost effectiveness for long-term vaccination/challenge studies. Dunkin Hartley guinea pigs are often used in M. tuberculosis studies as a close model for the overall pathogenesis in humans.
6. Host Protective Immunity
Although it is known that IFN-gamma is essential for protective immunity, animal and human studies have found that IFN-gamma alone is not sufficient for the prevention of TB disease. There is evidence that IL-23, a recently described member of the IL-12 family of cytokines, is important in the immuno-pathogenesis of TB. There is also evidence that regulatory T cells (Treg) are present in TB disease and that Treg may suppress effector T cell responses (Fletcher, 2007). New vaccines seek to target specific CD1, CD4+, and CD8+ T and B cell responses to assist in eradicating the disease via improved clearance of infected cells and bacterial loads.
1. Ag85A
  • Gene Name : Ag85A
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0010936
  • NCBI Gene ID : 886132
  • NCBI Protein GI : 15610940
  • Locus Tag : Rv3804c
  • Genbank Accession : BX842584
  • Protein Accession : NP_218321
  • Other Database IDs : LOCUS:AY207395.1;GI:29027586;GI:29027587
  • Taxonomy ID : 83332
  • Gene Starting Position : 4265641
  • Gene Ending Position : 4266657
  • Gene Strand (Orientation) : -
  • Protein Name : secreted antigen 85-A FbpA
  • Protein pI : 6.51
  • Protein Weight : 33304.71
  • Protein Length : 338
  • Protein Note : 2.3.1.-
  • DNA Sequence : Show Sequence
    >gi|57116681:4265641-4266657 Mycobacterium tuberculosis H37Rv chromosome, complete genome
    GCTAGGCGCCCTGGGGCGCGGGCCCGGTGTTGGGCGTGGCACCCAGTGCCCGTTGCAGGTCGGGCTTCAT
    AGCGTTGAGCTGCGCGCCCCAGTACTCCCAGCTGTGCGTACCGCTGTCCGGGAAGTCGAACACGCCGTTG
    TGGCCGCCACCGGCGTTGTAGGCGTCTTGGAACTTGATGTTGCTGGTCCGCACGAAGCCCTCGAGGAACT
    TGGCCGGCAGGTTGTTGCCACCCAGATCCGACGGCTTGCCGTTGCCGCAGTACACCCAGACGCGGGTGTT
    GTTGGCGATCAGCTTCCCGACGTTCAACAGCGGGTCGTTGCGCTGCCACGCCGGGTCCTCCTTCGGGCCC
    CACATGTCGGAGGCCTTGTAGCCGCCAGCGTCACCCATCGCCAGGCCGATCAGGGTGGGACCCATCGCCT
    GGGAGGGGTCCAACAGGCCCGACATCGCTCCCGCGTAGACGAACTGCTGGGGGTGATAGATCGCCAGCGT
    CAGCGCCGAAGAAGCAGCCATCGAAAGACCGACGACGGCGCTTCCGGTGGGCTTGACGTGCCTGTTGGCC
    TGCAGCCACCCCGGCAGCTCGCTGGTCAGGAAGGTCTCCCACTTGTAAGTCTGGCAACCGGCCTTGCCGC
    AGGCGGGCTGGTACCAGTCGGAGTAGAAGCTTGACTGGCCACCCACCGGCATGACCACCGACAGGCCCGA
    CTGGTCGTACCACTCGAACGCCGGGGTGTTGATGTCCCAGCCGCTGAAGTCGTCCTGCGCGCGCAGGCCG
    TCGAGCAGGTACAGGGCGGGCGAGTTGGCACCACCACTTTGGAATTGGACCTTGATGTCACGGCCCATCG
    ACGGCGACGGCACCTGCAGGTACTCCACCGGCAAGCCCGGCCGGGAAAATGCCCCCGCGGTCGCCGTGCC
    ACCGACGGCGCCGACCAGACCCGACACTAGGGCCGCGCCGACGGCCCCGACCACGAGTCGACGCGACATA
    CCCGTGACGGCGCCACGAACCCTGTCAACAAGCTGCA
  • Protein Sequence : Show Sequence
    >gi|15610940|ref|NP_218321.1| fbpA gene product [Mycobacterium tuberculosis H37Rv]
    MQLVDRVRGAVTGMSRRLVVGAVGAALVSGLVGAVGGTATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQ
    SGGANSPALYLLDGLRAQDDFSGWDINTPAFEWYDQSGLSVVMPVGGQSSFYSDWYQPACGKAGCQTYKW
    ETFLTSELPGWLQANRHVKPTGSAVVGLSMAASSALTLAIYHPQQFVYAGAMSGLLDPSQAMGPTLIGLA
    MGDAGGYKASDMWGPKEDPAWQRNDPLLNVGKLIANNTRVWVYCGNGKPSDLGGNNLPAKFLEGFVRTSN
    IKFQDAYNAGGGHNGVFDFPDSGTHSWEYWGAQLNAMKPDLQRALGATPNTGPAPQGA
  • Molecule Role : Protective antigen
  • Related Vaccine(s): DNA vaccine co-expressing A85A and caspase-3 , M. tuberculosis DNA vaccine DNAAg85A encoding a single immunogenic M.tb Ag , M. tuberculosis DNA vaccine pcDNA3.1+/Ag85A DNA encoding Ag85A , MVA/IL-15/5Mtb vaccine , rBCG-Ag85A/ Ag85B , rBCG-Ag85A[Tokyo]
2. Ag85B from M. tuberculosis H37Rv
  • Gene Name : Ag85B from M. tuberculosis H37Rv
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0012364
  • NCBI Gene ID : 885785
  • NCBI Protein GI : 15609023
  • Locus Tag : Rv1886c
  • Genbank Accession : NC_000962.2
  • Protein Accession : NC_000962.2
  • Other Database IDs : EMBL: AE000516
    EMBL: AF198032
    EMBL: BX842578
    EMBL: U38939
    EMBL: X62398
    GenomeReviews: AE000516_GR
    GenomeReviews: AL123456_GR
    InterPro: IPR000801
    KEGG: mtc:MT1934
    KEGG: mtu:Rv1886c
    PDB: 1F0N
    PDB: 1F0P
    Pfam: PF00756
    PIR: C70516
    TIGR: MT1934
    TubercuList: Rv1886c
    UniProt: P31952
  • Taxonomy ID : 1773
  • Gene Starting Position : 2134890
  • Gene Ending Position : 2135867
  • Gene Strand (Orientation) : -
  • Protein Name : SECRETED ANTIGEN 85-B FBPB (85B) (ANTIGEN 85 COMPLEX B) (MYCOLYL TRANSFERASE 85B) (FIBRONECTIN-BINDING PROTEIN B) (EXTRACELLULAR ALPHA-ANTIGEN)
  • Protein pI : 5.72
  • Protein Weight : 34580.6
  • Protein Length : 325
  • Protein Note : Rv1886c, (MT1934, MTCY180.32), len: 325 aa. fbpB (alternate gene names: mpt59, 85B), precursor of the 85-B antigen (fibronectin-binding protein B) (mycolyl transferase 85B) (EC 2.3.1.-) (see citations below), highly similar to other Mycobacterial antigen precursors e.g. P12942|A85B_MYCBO ANTIGEN 85-B PRECURSOR from Mycobacterium bovis (323 aa); P21160|A85B_MYCKA ANTIGEN 85-B PRECURSOR from Mycobacterium kansasii (325 aa); etc. Also highly similar to Mycobacterium tuberculosis antigen precursors: Rv3804c|fbpA (338 aa), Rv0129c|fbpC2 (340 aa), and Rv3803c|fbpC1 (299 aa). TBparse score is 0.912.; mpt59; 85B
  • Protein Annotation : FUNCTION: Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity (UniProt: P31952)

    SUBCELLULAR LOCATION: Secreted protein (UniProt: P31952)

    SIMILARITY: Belongs to the mycobacterial A85 antigen family (UniProt: P31952)
  • DNA Sequence : Show Sequence
    >GeneID|885785 [Mycobacterium tuberculosis H37Rv ] 2134890..2135867
    tcagccggcgcctaacgaactctgcaggtcacccttcatggcgttgagctgagcgccccagtactcccag
    ctgtgcgtgccgttgggcgggaagttgaacacggcgttgtgcccgcccgcggcgttgtacgcatcctgga
    acttcaggttgctgctacgaacgaagttctccaagaactcggcgggtatgttggcaccgcccaactcgtt
    cggggtgccgttcccgcaataaacccatagccgggtgttgtttgcgaccagcttggggatctgctgcgta
    gggtcgttgcgctcccatgccgggtcactcgagggaccccacatgtctgcggccttgtaaccgccggcgt
    cacccatcgcgaggccgatcaggctaggccccatcccctgagaggggtccagcagggccgacagcgagcc
    ggcgtagatgaactgctgggggtggtaggcggccaagatcattgccgacgagccggccatcgacaagccg
    attgcagcgctgccggtgggcttcacggccctgttggcggacaaccattgcggcagctcgctggtcagga
    aggtttcccacttgtaagtctggcagccagccttaccgcaggccgggctgtaccagtcgctgtagaagct
    ggactgcccgccgaccggcatgactatcgacagtcccgactggtagtaccactcgaacgccggggtgttg
    atatcccagccgttgtagtcgtcttgggcgcgcaggccgtcgagcagataaaccgcaggtgagttgttcc
    caccgctctggaactgaaccttgatgtcgcggcccatcgacggcgacggcacctgcaggtactcgaccgg
    cagccccggccgggagaacgcgcccgcggttgccgctccgccggcaagccccaccaggcccggaaggact
    acagccgctgccgtgccgatcatcaatcggcgtccccaagctcgaatctttcggctcacgtctgtcat
  • Protein Sequence : Show Sequence
    >gi|15609023|ref|NP_216402.1| SECRETED ANTIGEN 85-B FBPB (85B) (ANTIGEN 85 COMPLEX B) (MYCOLYL TRANSFERASE 85B) (FIBRONECTIN-BINDING PROTEIN B) (EXTRACELLULAR ALPHA-ANTIGEN) [Mycobacterium tuberculosis H37Rv ]
    MTDVSRKIRAWGRRLMIGTAAAVVLPGLVGLAGGAATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQSGG
    NNSPAVYLLDGLRAQDDYNGWDINTPAFEWYYQSGLSIVMPVGGQSSFYSDWYSPACGKAGCQTYKWETF
    LTSELPQWLSANRAVKPTGSAAIGLSMAGSSAMILAAYHPQQFIYAGSLSALLDPSQGMGPSLIGLAMGD
    AGGYKAADMWGPSSDPAWERNDPTQQIPKLVANNTRLWVYCGNGTPNELGGANIPAEFLENFVRSSNLKF
    QDAYNAAGGHNAVFNFPPNGTHSWEYWGAQLNAMKGDLQSSLGAG
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : In mice, a primary vaccination with Ag85B-encoding plasmid DNA (DNA-85B) was protective against Mycobacterium tuberculosis (MTB) infection and associated with Ag85B-specific CD4+ T cells producing IFN-gamma and controlling intramacrophagic MTB growth (Palma et al., 2007).
  • Related Vaccine(s): Ag85B and ESAT-6 fusion protein , Arabinomannan-tetanus toxoid conjugate , LT-BCG- Ag85B /Rv3425 , M. tuberculosis DNA vaccine encoding Ag85B, MPT64 and MPT83 , MVA/IL-15/5Mtb vaccine , rBCG-Ag85A/ Ag85B
3. Apa
  • Gene Name : Apa
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0011175
  • NCBI Gene ID : 885896
  • NCBI Protein GI : 57116926
  • Locus Tag : Rv1860
  • Genbank Accession : BX842578
  • Protein Accession : YP_177849
  • Taxonomy ID : 83332
  • Gene Starting Position : 2107735
  • Gene Ending Position : 2108712
  • Gene Strand (Orientation) : ?
  • Protein Name : hypothetical protein
  • Protein pI : 4.7
  • Protein Weight : 30636.65
  • Protein Length : 325
  • Protein Note : Rv1860, (MT1908, MTCY359.0013), len: 325 aa. apa (alternate gene names: mpt32, modD), Ala-, Pro-rich 45/47 kDa secreted protein, very similar to P46842|N43L_MYCLE from Mycobacterium leprae (287 aa), FASTA scores: opt: 1166, E(): 0, (66.4% identity in 298 aa overlap). Known to be glycosylated fibronectin-binding protein (see some citations). CHANGES IN THE MANNOSYLATION PATTERN OF THIS PROTEIN AFFECT ITS ABILITY TO STIMULATE T-LYMPHOCYTE RESPONSE. MAJOR IMMUNODOMINANT ANTIGEN THAT HAS POTENTIAL AS A VACCINE AGAINST TUBERCULOSIS. APA-ELISA COULD BE USED IN DIAGNOSIS. TBparse score is 0.924.; mpt32; modD
  • DNA Sequence : Show Sequence
    >gi|57116681:2107735-2108712 Mycobacterium tuberculosis H37Rv, complete genome
    CATGCATCAGGTGGACCCCAACTTGACACGTCGCAAGGGACGATTGGCGGCACTGGCTATCGCGGCGATG
    GCCAGCGCCAGCCTGGTGACCGTTGCGGTGCCCGCGACCGCCAACGCCGATCCGGAGCCAGCGCCCCCGG
    TACCCACAACGGCCGCCTCGCCGCCGTCGACCGCTGCAGCGCCACCCGCACCGGCGACACCTGTTGCCCC
    CCCACCACCGGCCGCCGCCAACACGCCGAATGCCCAGCCGGGCGATCCCAACGCAGCACCTCCGCCGGCC
    GACCCGAACGCACCGCCGCCACCTGTCATTGCCCCAAACGCACCCCAACCTGTCCGGATCGACAACCCGG
    TTGGAGGATTCAGCTTCGCGCTGCCTGCTGGCTGGGTGGAGTCTGACGCCGCCCACTTCGACTACGGTTC
    AGCACTCCTCAGCAAAACCACCGGGGACCCGCCATTTCCCGGACAGCCGCCGCCGGTGGCCAATGACACC
    CGTATCGTGCTCGGCCGGCTAGACCAAAAGCTTTACGCCAGCGCCGAAGCCACCGACTCCAAGGCCGCGG
    CCCGGTTGGGCTCGGACATGGGTGAGTTCTATATGCCCTACCCGGGCACCCGGATCAACCAGGAAACCGT
    CTCGCTCGACGCCAACGGGGTGTCTGGAAGCGCGTCGTATTACGAAGTCAAGTTCAGCGATCCGAGTAAG
    CCGAACGGCCAGATCTGGACGGGCGTAATCGGCTCGCCCGCGGCGAACGCACCGGACGCCGGGCCCCCTC
    AGCGCTGGTTTGTGGTATGGCTCGGGACCGCCAACAACCCGGTGGACAAGGGCGCGGCCAAGGCGCTGGC
    CGAATCGATCCGGCCTTTGGTCGCCCCGCCGCCGGCGCCGGCACCGGCTCCTGCAGAGCCCGCTCCGGCG
    CCGGCGCCGGCCGGGGAAGTCGCTCCTACCCCGACGACACCGACACCGCAGCGGACCTTACCGGCCTG
  • Protein Sequence : Show Sequence
    >gi|57116926|ref|YP_177849.1| hypothetical protein Rv1860 [Mycobacterium tuberculosis H37Rv]
    MHQVDPNLTRRKGRLAALAIAAMASASLVTVAVPATANADPEPAPPVPTTAASPPSTAAAPPAPATPVAP
    PPPAAANTPNAQPGDPNAAPPPADPNAPPPPVIAPNAPQPVRIDNPVGGFSFALPAGWVESDAAHFDYGS
    ALLSKTTGDPPFPGQPPPVANDTRIVLGRLDQKLYASAEATDSKAAARLGSDMGEFYMPYPGTRINQETV
    SLDANGVSGSASYYEVKFSDPSKPNGQIWTGVIGSPAANAPDAGPPQRWFVVWLGTANNPVDKGAAKALA
    ESIRPLVAPPPAPAPAPAEPAPAPAPAGEVAPTPTTPTPQRTLPA
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : We show that the 50- to 55-kDa Apa protein, specified by the Rv1860 gene of M. tuberculosis, can elicit both lymphoproliferative response and gamma interferon (IFN-gamma) production. A poxvirus recombinant expressing the Apa protein conferred a significant level of protective immunity in guinea pigs against a challenge dose of virulent M. tuberculosis (Kumar et al., 2003).
  • Related Vaccine(s): M. tuberculosis DNA Vaccine APADNA priming and APAMVA boosting
4. bfrA
  • Gene Name : bfrA
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 885767
  • NCBI Protein GI : 15609013
  • Locus Tag : Rv1876
  • Genbank Accession : CP003248
  • Protein Accession : NP_216392
  • Taxonomy ID : 83332
  • Gene Starting Position : 2125339
  • Gene Ending Position : 2125818
  • Gene Strand (Orientation) : +
  • Protein Name : bacterioferritin BfrA
  • Protein pI : 4.23
  • Protein Weight : 17744.75
  • Protein Length : 159
  • Protein Note : Also known as bfr
  • DNA Sequence : Show Sequence
    >gi|448814763:2125339-2125818 Mycobacterium tuberculosis H37Rv, complete genome
    CATGCAAGGTGATCCCGATGTTCTGCGCCTGCTCAACGAACAATTGACCAGCGAGCTCACCGCTATCAAC
    CAATACTTTCTGCACTCCAAGATGCAGGACAACTGGGGTTTTACCGAGCTGGCGGCCCACACCCGCGCGG
    AGTCGTTCGACGAAATGCGGCACGCCGAGGAAATCACCGATCGCATCTTGTTGCTGGATGGTTTGCCGAA
    CTACCAGCGCATCGGTTCGTTGCGTATCGGCCAGACGCTCCGCGAGCAATTTGAGGCCGATCTGGCGATC
    GAATACGACGTGTTGAATCGTCTCAAGCCAGGAATCGTCATGTGCCGGGAGAAACAGGACACCACCAGCG
    CCGTACTGCTGGAGAAAATCGTTGCCGACGAGGAAGAACACATCGACTACTTGGAAACGCAGCTGGAGCT
    GATGGACAAGCTAGGAGAGGAGCTTTACTCGGCGCAGTGCGTCTCTCGCCCACCGACCTG
  • Protein Sequence : Show Sequence
    >gi|15609013|ref|NP_216392.1| bacterioferritin BfrA [Mycobacterium tuberculosis H37Rv]
    MQGDPDVLRLLNEQLTSELTAINQYFLHSKMQDNWGFTELAAHTRAESFDEMRHAEEITDRILLLDGLPN
    YQRIGSLRIGQTLREQFEADLAIEYDVLNRLKPGIVMCREKQDTTSAVLLEKIVADEEEHIDYLETQLEL
    MDKLGEELYSAQCVSRPPT
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mycobacterium frA (or called MMP-II) is one of the immunodominant antigens (Mukai et al., 2014).
  • Related Vaccine(s): BCG-DHTM
5. DnaK
  • Gene Name : DnaK
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0012408
  • NCBI Gene ID : 885946
  • NCBI Protein GI : 15607491
  • Locus Tag : Rv0350
  • Genbank Accession : BX842573
  • Protein Accession : NP_214864
  • Taxonomy ID : 83332
  • Gene Starting Position : 419834
  • Gene Ending Position : 421711
  • Gene Strand (Orientation) : ?
  • Protein Name : molecular chaperone DnaK
  • Protein pI : 4.59
  • Protein Weight : 61176.37
  • Protein Length : 625
  • Protein Note : heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria
  • DNA Sequence : Show Sequence
    >gi|57116681:419834-421711 Mycobacterium tuberculosis H37Rv, complete genome
    CATGGCTCGTGCGGTCGGGATCGACCTCGGGACCACCAACTCCGTCGTCTCGGTTCTGGAAGGTGGCGAC
    CCGGTCGTCGTCGCCAACTCCGAGGGCTCCAGGACCACCCCGTCAATTGTCGCGTTCGCCCGCAACGGTG
    AGGTGCTGGTCGGCCAGCCCGCCAAGAACCAGGCAGTGACCAACGTCGATCGCACCGTGCGCTCGGTCAA
    GCGACACATGGGCAGCGACTGGTCCATAGAGATTGACGGCAAGAAATACACCGCGCCGGAGATCAGCGCC
    CGCATTCTGATGAAGCTGAAGCGCGACGCCGAGGCCTACCTCGGTGAGGACATTACCGACGCGGTTATCA
    CGACGCCCGCCTACTTCAATGACGCCCAGCGTCAGGCCACCAAGGACGCCGGCCAGATCGCCGGCCTCAA
    CGTGCTGCGGATCGTCAACGAGCCGACCGCGGCCGCGCTGGCCTACGGCCTCGACAAGGGCGAGAAGGAG
    CAGCGAATCCTGGTCTTCGACTTGGGTGGTGGCACTTTCGACGTTTCCCTGCTGGAGATCGGCGAGGGTG
    TGGTTGAGGTCCGTGCCACTTCGGGTGACAACCACCTCGGCGGCGACGACTGGGACCAGCGGGTCGTCGA
    TTGGCTGGTGGACAAGTTCAAGGGCACCAGCGGCATCGATCTGACCAAGGACAAGATGGCGATGCAGCGG
    CTGCGGGAAGCCGCCGAGAAGGCAAAGATCGAGCTGAGTTCGAGTCAGTCCACCTCGATCAACCTGCCCT
    ACATCACCGTCGACGCCGACAAGAACCCGTTGTTCTTAGACGAGCAGCTGACCCGCGCGGAGTTCCAACG
    GATCACTCAGGACCTGCTGGACCGCACTCGCAAGCCGTTCCAGTCGGTGATCGCTGACACCGGCATTTCG
    GTGTCGGAGATCGATCACGTTGTGCTCGTGGGTGGTTCGACCCGGATGCCCGCGGTGACCGATCTGGTCA
    AGGAACTCACCGGCGGCAAGGAACCCAACAAGGGCGTCAACCCCGATGAGGTTGTCGCGGTGGGAGCCGC
    TCTGCAGGCCGGCGTCCTCAAGGGCGAGGTGAAAGACGTTCTGCTGCTTGATGTTACCCCGCTGAGCCTG
    GGTATCGAGACCAAGGGCGGGGTGATGACCAGGCTCATCGAGCGCAACACCACGATCCCCACCAAGCGGT
    CGGAGACTTTCACCACCGCCGACGACAACCAACCGTCGGTGCAGATCCAGGTCTATCAGGGGGAGCGTGA
    GATCGCCGCGCACAACAAGTTGCTCGGGTCCTTCGAGCTGACCGGCATCCCGCCGGCGCCGCGGGGGATT
    CCGCAGATCGAGGTCACTTTCGACATCGACGCCAACGGCATTGTGCACGTCACCGCCAAGGACAAGGGCA
    CCGGCAAGGAGAACACGATCCGAATCCAGGAAGGCTCGGGCCTGTCCAAGGAAGACATTGACCGCATGAT
    CAAGGACGCCGAAGCGCACGCCGAGGAGGATCGCAAGCGTCGCGAGGAGGCCGATGTTCGTAATCAAGCC
    GAGACATTGGTCTACCAGACGGAGAAGTTCGTCAAAGAACAGCGTGAGGCCGAGGGTGGTTCGAAGGTAC
    CTGAAGACACGCTGAACAAGGTTGATGCCGCGGTGGCGGAAGCGAAGGCGGCACTTGGCGGATCGGATAT
    TTCGGCCATCAAGTCGGCGATGGAGAAGCTGGGCCAGGAGTCGCAGGCTCTGGGGCAAGCGATCTACGAA
    GCAGCTCAGGCTGCGTCACAGGCCACTGGCGCTGCCCACCCCGGCGGCGAGCCGGGCGGTGCCCACCCCG
    GCTCGGCTGATGACGTTGTGGACGCGGAGGTGGTCGACGACGGCCGGGAGGCCAAGTG
  • Protein Sequence : Show Sequence
    >gi|15607491|ref|NP_214864.1| molecular chaperone DnaK [Mycobacterium tuberculosis H37Rv]
    MARAVGIDLGTTNSVVSVLEGGDPVVVANSEGSRTTPSIVAFARNGEVLVGQPAKNQAVTNVDRTVRSVK
    RHMGSDWSIEIDGKKYTAPEISARILMKLKRDAEAYLGEDITDAVITTPAYFNDAQRQATKDAGQIAGLN
    VLRIVNEPTAAALAYGLDKGEKEQRILVFDLGGGTFDVSLLEIGEGVVEVRATSGDNHLGGDDWDQRVVD
    WLVDKFKGTSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINLPYITVDADKNPLFLDEQLTRAEFQR
    ITQDLLDRTRKPFQSVIADTGISVSEIDHVVLVGGSTRMPAVTDLVKELTGGKEPNKGVNPDEVVAVGAA
    LQAGVLKGEVKDVLLLDVTPLSLGIETKGGVMTRLIERNTTIPTKRSETFTTADDNQPSVQIQVYQGERE
    IAAHNKLLGSFELTGIPPAPRGIPQIEVTFDIDANGIVHVTAKDKGTGKENTIRIQEGSGLSKEDIDRMI
    KDAEAHAEEDRKRREEADVRNQAETLVYQTEKFVKEQREAEGGSKVPEDTLNKVDAAVAEAKAALGGSDI
    SAIKSAMEKLGQESQALGQAIYEAAQAASQATGAAHPGGEPGGAHPGSADDVVDAEVVDDGREAK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : In an ex vivo transfection approach with a retroviral vector a single antigen (hsp70) could evoke strong protection in mice (Lowrie et al., 1997).
  • Related Vaccine(s): BCG-DHTM
6. drrC
  • Gene Name : drrC
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 888491
  • NCBI Protein GI : 15610075
  • Locus Tag : Rv2938
  • Genbank Accession : BX842581
  • Protein Accession : NP_217454
  • Taxonomy ID : 83332
  • Gene Starting Position : 3274071
  • Gene Ending Position : 3274901
  • Gene Strand (Orientation) : ?
  • Protein Name : daunorubicin-DIM-transport integral membrane protein ABC transporter DrrC
  • Protein pI : 10.46
  • Protein Weight : 26320.25
  • Protein Length : 276
  • Protein Note : Rv2938, (MTCY19H9.06), len: 276 aa. Probable drrC, daunorubicin-DIM-transport integral membrane protein ABC transporter, probably involved in daunorubicin resistance and phthiocerol dimycocerosate transport (see citations below), equivalent to Q9CB71|ML2350 PROBABLE ANTIBIOTIC RESISTANCE MEMBRANE PROTEIN from Mycobacterium leprae (276 aa), FASTA scores: opt: 1434, E(): 1.2e-81, (79.0% identity in 276 aa overlap); and Q49941|DRRC|L518_F3_76 PUTATIVE DAUNORUBICIN RESISTANCE TRANSMEMBRANE PROTEIN from Mycobacterium leprae (244 aa), FASTA scores: opt: 1194, E(): 8.3e-67, (76.85% identity in 242 aa overlap). Also similar to others e.g. Q9XCF9 DRRC PROTEIN from Mycobacterium avium (263 aa), FASTA scores: opt: 538, E(): 3.7e-26, (32.65% identity in 251 aa overlap); Q9S6H3 DAUNORUBICIN RESISTANCE PROTEIN C from Mycobacterium avium (263 aa), FASTA scores: opt: 533, E(): 7.6e-26, (32.25% identity in 251 aa overlap); P32011|DRRB_STRPE DAUNORUBICIN RESISTANCE TRANSMEMBRANE PROTEIN from Streptomyces peucetius (283 aa), FASTA scores: opt: 276, E(): 6.6e-10, (21.07% identity in 261 aa overlap); etc. Note that Rv2938|drrC belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentaly).
  • DNA Sequence : Show Sequence
    >gi|57116681:3274071-3274901 Mycobacterium tuberculosis H37Rv, complete genome
    CATGATCACGACGACAAGTCAGGAAATCGAGCTTGCACCCACACGTTTGCCAGGCTCGCAAAACGCTGCT
    CGGCTGTTCGTTGCGCAGACCCTTTTGCAGACCAACCGGTTGCTAACTCGATGGGCACGTGACTATATCA
    CCGTTATCGGAGCGATCGTGTTACCGATTCTCTTCATGGTGGTGTTGAACATTGTGCTAGGTAACCTAGC
    TTATGTCGTAACCCACGACAGCGGGCTCTACAGCATTGTTCCGCTGATCGCACTCGGCGCCGCGATCACT
    GGGTCAACTTTTGTCGCGATCGACCTGATGCGCGAGCGCTCCTTCGGACTGCTTGCCCGACTGTGGGTGC
    TGCCCGTGCACCGAGCATCGGGCCTGATCTCTCGAATCCTGGCAAACGCGATTCGGACTCTGGTCACCAC
    TTTAGTGATGCTAGGTACTGGGGTGGTATTGGGTTTCCGGTTTCGACAAGGCCTGATCCCGAGCCTCATG
    TGGATTAGTGTCCCGGTGATACTGGGCATCGCAATCGCGGCTATGGTCACTACCGTCGCGCTTTACACAG
    CACAAACCGTTGTTGTCGAAGGCGTTGAGCTGGTGCAAGCAATCGCGATCTTCTTCTCCACGGGTTTGGT
    GCCGCTCAACTCGTATCCAGGCTGGATTCAGCCGTTCGTCGCCCATCAGCCGGTGAGCTACGCCATCGCG
    GCGATGCGCGGTTTTGCAATGGGTGGTCCGGTCCTCTCTCCGATGATCGGGATGCTGGTGTGGACCGCGG
    GTATCTGCGTCGTATGCGCCGTACCCTTGGCCATTGGCTACCGACGGGCCAGCACGCATTG
  • Protein Sequence : Show Sequence
    >gi|15610075|ref|NP_217454.1| daunorubicin-DIM-transport integral membrane protein ABC transporter DrrC [Mycobacterium tuberculosis H37Rv]
    MITTTSQEIELAPTRLPGSQNAARLFVAQTLLQTNRLLTRWARDYITVIGAIVLPILFMVVLNIVLGNLA
    YVVTHDSGLYSIVPLIALGAAITGSTFVAIDLMRERSFGLLARLWVLPVHRASGLISRILANAIRTLVTT
    LVMLGTGVVLGFRFRQGLIPSLMWISVPVILGIAIAAMVTTVALYTAQTVVVEGVELVQAIAIFFSTGLV
    PLNSYPGWIQPFVAHQPVSYAIAAMRGFAMGGPVLSPMIGMLVWTAGICVVCAVPLAIGYRRASTH
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A drrC mutant is highly attenuated in mice and induces significant protection from challenge with wild type M. tuberculosis (Pinto et al., 2004).
  • Related Vaccine(s): Mycobacterium tuberculosis drrC mutant vaccine
7. ESAT-6
  • Gene Name : ESAT-6
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0012368
  • NCBI Gene ID : 886209
  • NCBI Protein GI : 57117165
  • Locus Tag : Rv3875
  • Genbank Accession : FJ014499
  • Protein Accession : YP_178023
  • Taxonomy ID : 83332
  • Gene Starting Position : 4352608
  • Gene Ending Position : 4352895
  • Gene Strand (Orientation) : ?
  • Protein Name : 6 kDa early secretory antigenic target ESXA (ESAT-6)
  • Protein pI : 4.19
  • Protein Weight : 9605.81
  • Protein Length : 95
  • Protein Note : Rv3875, (MT3989, MTV027.10), len: 95 aa. esxA, early secretory antigenic target (see citations below), identical to Q57165|O84901|ESAT6 EARLY SECRETORY ANTIGENIC TARGET from Mycobacterium bovis (94 aa), FASTA scores: opt: 596, E(): 4.6e-33, (100.0% identity in 94 aa overlap). Also similar to Q50206|ESA6_MYCLE|ESAT6|ESX|L45|ML0049|MLCB628.12c 6 KDA EARLY SECRETORY ANTIGENIC TARGET HOMOLOG (ESAT-6-LIKE PROTEIN) (L-ESAT) from Mycobacterium leprae (95 aa), FASTA scores: opt: 236, E(): 3.3e-09, (36.25% identity in 91 aa overlap); and weak similarity with others proteins ESAT-like from Mycobacterium leprae. Also some similarity with O53266|ES69_MYCTU|Rv3019c|MT3104|MTV012.33c PUTATIVE SECRETED ESAT-6 LIKE PROTEIN 9 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 131, E(): 0.03, (26.15% identity in 88 aa overlap); and other ESAT-like protein. Contains probable coiled-coil from 56 to 92 aa. BELONGS TO THE ESAT6 FAMILY. Note that previously known as esat-6.; esat-6
  • DNA Sequence : Show Sequence
    >gi|57116681:4352608-4352895 Mycobacterium tuberculosis H37Rv, complete genome
    CATGACAGAGCAGCAGTGGAATTTCGCGGGTATCGAGGCCGCGGCAAGCGCAATCCAGGGAAATGTCACG
    TCCATTCATTCCCTCCTTGACGAGGGGAAGCAGTCCCTGACCAAGCTCGCAGCGGCCTGGGGCGGTAGCG
    GTTCGGAGGCGTACCAGGGTGTCCAGCAAAAATGGGACGCCACGGCTACCGAGCTGAACAACGCGCTGCA
    GAACCTGGCGCGGACGATCAGCGAAGCCGGTCAGGCAATGGCTTCGACCGAAGGCAACGTCACTGGGATG
    TTCGCATA
  • Protein Sequence : Show Sequence
    >gi|57117165|ref|YP_178023.1| 6 kDa early secretory antigenic target ESXA (ESAT-6) [Mycobacterium tuberculosis H37Rv]
    MTEQQWNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQ
    NLARTISEAGQAMASTEGNVTGMFA
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : In a prophylactic vaccine model, immunization with the Mycobacterium tuberculosis protein ESAT-6 with LANAC adjuvant elicited significant protective immunity against aerosol challenge with virulent M. tuberculosis (Zaks et al., 2006).
8. EsxA (ESAT-6)
  • Gene Name : EsxA (ESAT-6)
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0010919
  • NCBI Gene ID : 886209
  • NCBI Protein GI : 57117165
  • Locus Tag : Rv3875
  • Genbank Accession : NC_000962.2
  • Protein Accession : NC_000962.2
  • Other Database IDs : EMBL: AE000516
    EMBL: AF004671
    EMBL: AF420491
    EMBL: BX842584
    EMBL: X79562
    GenomeReviews: AE000516_GR
    GenomeReviews: AL123456_GR
    InterPro: IPR010310
    KEGG: mtc:MT3989
    KEGG: mtu:Rv3875
    PDB: 1WA8
    Pfam: PF06013
    PIR: A70803
    TIGR: MT3989
    TubercuList: Rv3875
    UniProt: P0A564
  • Taxonomy ID : 1773
  • Gene Starting Position : 4352609
  • Gene Ending Position : 4352896
  • Gene Strand (Orientation) : +
  • Protein Name : 6 KDA EARLY SECRETORY ANTIGENIC TARGET ESXA (ESAT-6)
  • Protein pI : 4.19
  • Protein Weight : 9903.8
  • Protein Length : 95
  • Protein Note : Rv3875, (MT3989, MTV027.10), len: 95 aa. esxA, early secretory antigenic target (see citations below), identical to Q57165|O84901|ESAT6 EARLY SECRETORY ANTIGENIC TARGET from Mycobacterium bovis (94 aa), FASTA scores: opt: 596, E(): 4.6e-33, (100.0% identity in 94 aa overlap). Also similar to Q50206|ESA6_MYCLE|ESAT6|ESX|L45|ML0049|MLCB628.12c 6 KDA EARLY SECRETORY ANTIGENIC TARGET HOMOLOG (ESAT-6-LIKE PROTEIN) (L-ESAT) from Mycobacterium leprae (95 aa), FASTA scores: opt: 236, E(): 3.3e-09, (36.25% identity in 91 aa overlap); and weak similarity with others proteins ESAT-like from Mycobacterium leprae. Also some similarity with O53266|ES69_MYCTU|Rv3019c|MT3104|MTV012.33c PUTATIVE SECRETED ESAT-6 LIKE PROTEIN 9 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 131, E(): 0.03, (26.15% identity in 88 aa overlap); and other ESAT-like protein. Contains probable coiled-coil from 56 to 92 aa. BELONGS TO THE ESAT6 FAMILY. Note that previously known as esat-6.; esat-6
  • Protein Annotation : FUNCTION: Not known. Elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response (UniProt: P0A564)

    SUBCELLULAR LOCATION: Secreted protein (UniProt: P0A564)

    SIMILARITY: Belongs to the ESAT-6 (esx) family (UniProt: P0A564)
  • DNA Sequence : Show Sequence
    >GeneID|886209 [Mycobacterium tuberculosis H37Rv ] 4352609..4352896
    atgacagagcagcagtggaatttcgcgggtatcgaggccgcggcaagcgcaatccagggaaatgtcacgt
    ccattcattccctccttgacgaggggaagcagtccctgaccaagctcgcagcggcctggggcggtagcgg
    ttcggaggcgtaccagggtgtccagcaaaaatgggacgccacggctaccgagctgaacaacgcgctgcag
    aacctggcgcggacgatcagcgaagccggtcaggcaatggcttcgaccgaaggcaacgtcactgggatgt
    tcgcatag
  • Protein Sequence : Show Sequence
    >gi|57117165|ref|YP_178023.1| 6 KDA EARLY SECRETORY ANTIGENIC TARGET ESXA (ESAT-6) [Mycobacterium tuberculosis H37Rv ]
    MTEQQWNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQ
    NLARTISEAGQAMASTEGNVTGMFA
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : The heterologous prime-boost strategy of live Salmonella vaccine expressing Mycobacterium tuberculosis fusion antigen Age5B-ESAT6 (esxA) from the chromosome followed by a systemic boost of antigen and adjuvant reduced the levels of M. tuberculosis bacteria in the lungs and spleen to the same extent as BCG. Additionally, this vaccination regimen was observed to be statistically equivalent in terms of protection to immunisation with BCG (Hall et al., 2009).
    In a prophylactic vaccine model, immunization with the Mycobacterium tuberculosis protein ESAT-6 with LANAC adjuvant elicited significant protective immunity against aerosol challenge with virulent M. tuberculosis (Zaks et al., 2006).
  • Related Vaccine(s): Ag85B and ESAT-6 fusion protein , attenuated M. bovis strain WAg539 , MVA/IL-15/5Mtb vaccine , recombinant S. typhimurium secreting M. tuberculosis ESAT-6
9. EsxB
  • Gene Name : EsxB
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0010951
  • NCBI Gene ID : 886194
  • NCBI Protein GI : 15611010
  • Locus Tag : Rv3874
  • Genbank Accession : BX842584
  • Protein Accession : NP_218391
  • 3D structure: PDB ID : 3FAV
  • Other Database IDs : EMBL: AE000516
    EMBL: AF004671
    EMBL: AF419854
    EMBL: BX842584
    GenomeReviews: AE000516_GR
    GenomeReviews: AL123456_GR
    InterPro: IPR010310
    KEGG: mtc:MT3988
    KEGG: mtu:Rv3874
    Pfam: PF06013
    PIR: H70802
    SMR: P0A566
    TIGR: MT3988
    TubercuList: Rv3874
    UniProt: P0A566
  • Taxonomy ID : 83332
  • Gene Starting Position : 4352273
  • Gene Ending Position : 4352575
  • Gene Strand (Orientation) : +
  • Protein Name : 10 kDa culture filtrate antigen EsxB
  • Protein pI : 4.31
  • Protein Weight : 10396.66
  • Protein Length : 100
  • Protein Note : Rv3874, (MT3988, MTV027.09), len: 100 aa. esxB, 10 KDA culture filtrate antigen (see citations below, especially first), highly similar to O33084|CF10_MYCLE|ML0050|MLCB628.13c 10 KDA CULTURE FILTRATE ANTIGEN CFP10 HOMOLOG from Mycobacterium leprae (99 aa), FASTA scores: opt: 237, E(): 2.4e-08, (39.4% identity in 99 aa overlap). Also similar to O05440|ES6D_MYCTU|Rv3905c|MT4024|MTCY15F10.06 PUTATIVE ESAT-6 LIKE PROTEIN 13 from Mycobacterium tuberculosis (103 aa) FASTA scores: opt: 126, E(): 0.18, (23.1% identity in 91 aa overlap); and shows some similarity with other proteins from Mycobacterium tuberculosis. Contains probable coiled-coil from aa 49-93. BELONGS TO THE ESAT6 FAMILY. Note that previously known as lhp (alternate gene name: cfp10).; lhp, cfp10
  • Protein Annotation : SUBCELLULAR LOCATION: Secreted protein (UniProt: P0A566)

    SIMILARITY: Belongs to the ESAT-6 (esx) family (UniProt: P0A566)
  • DNA Sequence : Show Sequence
    >gi|57116681:4352273-4352575 Mycobacterium tuberculosis H37Rv, complete genome
    CATGGCAGAGATGAAGACCGATGCCGCTACCCTCGCGCAGGAGGCAGGTAATTTCGAGCGGATCTCCGGC
    GACCTGAAAACCCAGATCGACCAGGTGGAGTCGACGGCAGGTTCGTTGCAGGGCCAGTGGCGCGGCGCGG
    CGGGGACGGCCGCCCAGGCCGCGGTGGTGCGCTTCCAAGAAGCAGCCAATAAGCAGAAGCAGGAACTCGA
    CGAGATCTCGACGAATATTCGTCAGGCCGGCGTCCAATACTCGAGGGCCGACGAGGAGCAGCAGCAGGCG
    CTGTCCTCGCAAATGGGCTTCTG
  • Protein Sequence : Show Sequence
    >gi|15611010|ref|NP_218391.1| 10 kDa culture filtrate antigen EsxB [Mycobacterium tuberculosis H37Rv]
    MAEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELD
    EISTNIRQAGVQYSRADEEQQQALSSQMGF
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : The 10-kDa culture filtrate protein (CFP-10, esxB) and 6-kDa early secretory antigen of T cells (ESAT-6) are secreted in abundance by Mycobacterium tuberculosis. Study demonstrated that protective immunity is induced by CFP-10 DNA vaccination in C3H mice as measured by a CFU reduction in the lung and spleen 4 and 8 weeks after challenge with M. tuberculosis (Wu et al., 2008).
  • Related Vaccine(s): attenuated M. bovis strain WAg539
10. fadD26
  • Gene Name : fadD26
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 887603
  • NCBI Protein GI : 57117038
  • Locus Tag : Rv2930
  • Genbank Accession : BX842581
  • Protein Accession : NP_217446
  • Taxonomy ID : 83332
  • Gene Starting Position : 3243696
  • Gene Ending Position : 3245447
  • Gene Strand (Orientation) : ?
  • Protein Name : acyl-CoA synthetase
  • Protein pI : 6.2
  • Protein Weight : 57587.87
  • Protein Length : 583
  • Protein Note : activates fatty acids by binding to coenzyme A
  • DNA Sequence : Show Sequence
    >gi|57116681:3243696-3245447 Mycobacterium tuberculosis H37Rv, complete genome
    GATGCCGGTGACCGACCGTTCAGTGCCCTCTTTGCTGCAAGAGAGGGCCGACCAGCAGCCTGACAGCACT
    GCATATACGTACATCGACTACGGATCCGACCCCAAGGGATTTGCTGACAGCTTGACTTGGTCGCAGGTCT
    ACAGTCGTGCATGCATCATTGCTGAAGAACTCAAGTTATGCGGGTTACCCGGAGATCGAGTGGCGGTTTT
    AGCGCCACAAGGACTGGAATATGTCCTTGCATTCCTGGGCGCACTTCAGGCTGGATTTATCGCGGTTCCG
    CTGTCAACTCCACAGTATGGCATTCACGATGACCGCGTTTCTGCGGTGTTGCAGGATTCCAAGCCGGTAG
    CCATTCTCACGACTTCGTCCGTGGTAGGCGATGTAACGAAATACGCAGCCAGCCACGACGGGCAGCCTGC
    CCCGGTCGTAGTTGAGGTTGATCTGCTTGATTTGGACTCGCCGCGACAGATGCCGGCTTTCTCTCGTCAG
    CACACCGGGGCGGCTTATCTCCAATACACGTCCGGATCGACGCGTACGCCGGCCGGAGTCATTGTGTCGC
    ACACGAATGTCATTGCCAATGTGACACAAAGTATGTACGGCTATTTCGGCGATCCCGCAAAGATTCCGAC
    CGGGACTGTGGTGTCGTGGCTGCCTTTGTATCACGATATGGGCCTGATTCTCGGAATTTGCGCACCGCTG
    GTGGCCCGACGCCGCGCGATGTTGATGAGCCCAATGTCATTTTTGCGCCGTCCGGCCCGCTGGATGCAAC
    TGCTTGCCACCAGCGGCCGGTGCTTTTCTGCGGCACCGAATTTCGCCTTCGAGCTGGCCGTGCGCAGAAC
    ATCTGACCAGGACATGGCGGGGCTCGACCTGCGCGACGTGGTCGGCATCGTCAGTGGCAGTGAGCGAATC
    CATGTGGCAACCGTGCGGCGGTTCATCGAGCGGTTCGCGCCGTACAATCTCAGCCCCACCGCGATACGGC
    CGTCGTACGGGCTCGCGGAAGCGACCTTATATGTGGCAGCTCCCGAAGCCGGCGCCGCGCCCAAGACGGT
    CCGTTTTGACTACGAGCAGCTGACCGCCGGGCAGGCTCGGCCCTGCGGAACCGATGGGTCGGTCGGCACC
    GAACTGATCAGCTACGGCTCCCCCGACCCATCGTCTGTGCGAATCGTCAACCCGGAGACCATGGTTGAGA
    ATCCGCCTGGAGTGGTCGGTGAGATCTGGGTGCATGGCGACCACGTGACTATGGGGTATTGGCAGAAGCC
    GAAGCAGACCGCGCAGGTCTTCGACGCCAAGCTGGTCGATCCCGCGCCGGCAGCCCCGGAGGGGCCGTGG
    CTGCGCACCGGCGACCTGGGCGTCATTTCCGATGGTGAGCTGTTCATCATGGGCCGCATCAAAGACCTGC
    TCATCGTGGACGGGCGCAACCACTACCCCGACGACATCGAGGCAACGATCCAGGAGATCACCGGTGGACG
    GGCCGCGGCGATCGCAGTGCCCGACGACATCACCGAACAACTGGTGGCGATCATCGAATTCAAGCGACGC
    GGTAGTACCGCCGAAGAGGTCATGCTCAAGCTCCGCTCGGTGAAGCGTGAGGTCACCTCCGCGATATCGA
    AGTCACACAGCCTGCGGGTGGCCGATCTCGTTCTGGTGTCACCTGGTTCGATTCCCATCACCACCAGCGG
    CAAGATCCGGCGGTCAGCCTGCGTCGAACGCTATCGCAGCGACGGCTTCAAGCGGCTGGACGTAGCCGTA
    TG
  • Protein Sequence : Show Sequence
    >gi|57117038|ref|NP_217446.2| acyl-CoA synthetase [Mycobacterium tuberculosis H37Rv]
    MPVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLCGLPGDRVAVL
    APQGLEYVLAFLGALQAGFIAVPLSTPQYGIHDDRVSAVLQDSKPVAILTTSSVVGDVTKYAASHDGQPA
    PVVVEVDLLDLDSPRQMPAFSRQHTGAAYLQYTSGSTRTPAGVIVSHTNVIANVTQSMYGYFGDPAKIPT
    GTVVSWLPLYHDMGLILGICAPLVARRRAMLMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRRT
    SDQDMAGLDLRDVVGIVSGSERIHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTV
    RFDYEQLTAGQARPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKP
    KQTAQVFDAKLVDPAPAAPEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIEATIQEITGGR
    AAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPITTSG
    KIRRSACVERYRSDGFKRLDVAV
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An fadD26 mutant is attenuated in mice and induced significant protection from challenge with wild type M. tuberculosis (Infante et al., 2005).
  • Related Vaccine(s): Mycobacterium tuberculosis fadD26 mutant vaccine
11. FbpA (Ag85A)
  • Gene Name : FbpA (Ag85A)
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0010952
  • NCBI Gene ID : 886132
  • NCBI Protein GI : 15610940
  • Locus Tag : Rv3804c
  • Genbank Accession : NC_000962.2
  • Protein Accession : NC_000962.2
  • 3D structure: PDB ID : 1SFR
  • Other Database IDs : EMBL: AE000516
    EMBL: BX842584
    EMBL: M27016
    EMBL: U47335
    GenomeReviews: AE000516_GR
    GenomeReviews: AL123456_GR
    InterPro: IPR000801
    KEGG: mtc:MT3911
    KEGG: mtu:Rv3804c
    PDB: 1SFR
    Pfam: PF00756
    PIR: H70887
    TIGR: MT3911
    TubercuList: Rv3804c
    UniProt: P0A4V2
  • Taxonomy ID : 1773
  • Gene Starting Position : 4265642
  • Gene Ending Position : 4266658
  • Gene Strand (Orientation) : -
  • Protein Name : SECRETED ANTIGEN 85-A FBPA (MYCOLYL TRANSFERASE 85A) (FIBRONECTIN-BINDING PROTEIN A) (ANTIGEN 85 COMPLEX A)
  • Protein pI : 6.51
  • Protein Weight : 35686
  • Protein Length : 338
  • Protein Note : Rv3804c, (MT3911, MTV026.09c), len: 338 aa. fbpA (alternate gene names: mpt44, 85A), precursor of the 85-A antigen (fibronectin-binding protein A) (mycolyl transferase 85A) (EC 2.3.1.-) (see citations below), identical to P17944|P17996|FBPA|MPT44 ANTIGEN 85-A PRECURSOR from Mycobacterium bovis (338 aa), FASTA scores: opt: 2341, E(): 1.2e-132, (100.0% identity in 338 aa overlap); and highly similar to other Mycobacterial antigen precursors e.g. O52956|A85A_MYCAV|FBPA ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium avium (347 aa), FASTA scores: opt: 1987, E(): 1.7e-111, (82.55% identity in 338 aa overlap); Q05861|A85A_MYCLE|FBPA|ML0097 ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium leprae (330 aa), FASTA scores: opt: 1936, E(): 1.9e-108, (83.0% identity in 329 aa overlap); O06052|A85A_MYCGO|FBPA ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium gordonae (339 aa), FASTA scores: opt: 1932, E(): 3.3e-108, (80.45% identity in 338 aa overlap); etc. Also highly similar to P31952|A85B_MYCTU|FBPB|Rv1886c|MT1934|MTCY180.32 SECRETED ANTIGEN 85-B from Mycobacterium tuberculosis (325 aa), FASTA scores: opt: 1830, E(): 3.9e-102, (78.85% identity in 317 aa overlap); P31953|A85C_MYCTU|FBPC|MPT45|Rv0129c|MTCI5.03c|FBPC2 SECRETED ANTIGEN 85-C from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 1597, E(): 3.4e-88, (67.25% identity in 336 aa overlap).; mpt44; 85A
  • Protein Annotation : FUNCTION: Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity (UniProt: P0A4V2)

    SUBCELLULAR LOCATION: Secreted protein (UniProt: P0A4V2)

    PTM: Contains one disulfide bridge (UniProt: P0A4V2)

    SIMILARITY: Belongs to the mycobacterial A85 antigen family (UniProt: P0A4V2)
  • DNA Sequence : Show Sequence
    >GeneID|886132 [Mycobacterium tuberculosis H37Rv ] 4265642..4266658
    ctaggcgccctggggcgcgggcccggtgttgggcgtggcacccagtgcccgttgcaggtcgggcttcata
    gcgttgagctgcgcgccccagtactcccagctgtgcgtaccgctgtccgggaagtcgaacacgccgttgt
    ggccgccaccggcgttgtaggcgtcttggaacttgatgttgctggtccgcacgaagccctcgaggaactt
    ggccggcaggttgttgccacccagatccgacggcttgccgttgccgcagtacacccagacgcgggtgttg
    ttggcgatcagcttcccgacgttcaacagcgggtcgttgcgctgccacgccgggtcctccttcgggcccc
    acatgtcggaggccttgtagccgccagcgtcacccatcgccaggccgatcagggtgggacccatcgcctg
    ggaggggtccaacaggcccgacatcgctcccgcgtagacgaactgctgggggtgatagatcgccagcgtc
    agcgccgaagaagcagccatcgaaagaccgacgacggcgcttccggtgggcttgacgtgcctgttggcct
    gcagccaccccggcagctcgctggtcaggaaggtctcccacttgtaagtctggcaaccggccttgccgca
    ggcgggctggtaccagtcggagtagaagcttgactggccacccaccggcatgaccaccgacaggcccgac
    tggtcgtaccactcgaacgccggggtgttgatgtcccagccgctgaagtcgtcctgcgcgcgcaggccgt
    cgagcaggtacagggcgggcgagttggcaccaccactttggaattggaccttgatgtcacggcccatcga
    cggcgacggcacctgcaggtactccaccggcaagcccggccgggaaaatgcccccgcggtcgccgtgcca
    ccgacggcgccgaccagacccgacactagggccgcgccgacggccccgaccacgagtcgacgcgacatac
    ccgtgacggcgccacgaaccctgtcaacaagctgcat
  • Protein Sequence : Show Sequence
    >gi|15610940|ref|NP_218321.1| SECRETED ANTIGEN 85-A FBPA (MYCOLYL TRANSFERASE 85A) (FIBRONECTIN-BINDING PROTEIN A) (ANTIGEN 85 COMPLEX A) [Mycobacterium tuberculosis H37Rv ]
    MQLVDRVRGAVTGMSRRLVVGAVGAALVSGLVGAVGGTATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQ
    SGGANSPALYLLDGLRAQDDFSGWDINTPAFEWYDQSGLSVVMPVGGQSSFYSDWYQPACGKAGCQTYKW
    ETFLTSELPGWLQANRHVKPTGSAVVGLSMAASSALTLAIYHPQQFVYAGAMSGLLDPSQAMGPTLIGLA
    MGDAGGYKASDMWGPKEDPAWQRNDPLLNVGKLIANNTRVWVYCGNGKPSDLGGNNLPAKFLEGFVRTSN
    IKFQDAYNAGGGHNGVFDFPDSGTHSWEYWGAQLNAMKPDLQRALGATPNTGPAPQGA
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Researchers have developed DNA vaccines expressing M. tuberculosis antigen 85A (Ag85A, fbpA) and cytokine granulocyte macrophage colony stimulating factor (GM-CSF). The immune strategy of BCG-prime and DNA vaccine boost induced mice to generate efficient immune protection against M. tuberculosis challenge (Dou et al., 2010).
  • Related Vaccine(s): Recombinant BCG, Ag85A in multiple vectors
12. FbpB from M. tuberculosis
  • Gene Name : FbpB from M. tuberculosis
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0011172
  • NCBI Gene ID : 885785
  • NCBI Protein GI : 15609023
  • Locus Tag : Rv1886c
  • Genbank Accession : BX842578
  • Protein Accession : NP_216402
  • 3D structure: PDB ID : 1F0N
  • Taxonomy ID : 83332
  • Gene Starting Position : 2134889
  • Gene Ending Position : 2135866
  • Gene Strand (Orientation) : -
  • Protein Name : secreted antigen 85-B fbpB (85B) (antigen 85 complex B) (Mycolyl transferase 85B) (fibronectin-binding protein B) (extracellular alpha-antigen)
  • Protein pI : 5.72
  • Protein Weight : 33091.46
  • Protein Length : 325
  • Protein Note : Rv1886c, (MT1934, MTCY180.32), len: 325 aa. fbpB (alternate gene names: mpt59, 85B), precursor of the 85-B antigen (fibronectin-binding protein B) (mycolyl transferase 85B) (EC 2.3.1.-) (see citations below), highly similar to other Mycobacterial antigen precursors e.g. P12942|A85B_MYCBO ANTIGEN 85-B PRECURSOR from Mycobacterium bovis (323 aa); P21160|A85B_MYCKA ANTIGEN 85-B PRECURSOR from Mycobacterium kansasii (325 aa); etc. Also highly similar to Mycobacterium tuberculosis antigen precursors: Rv3804c|fbpA (338 aa), Rv0129c|fbpC2 (340 aa), and Rv3803c|fbpC1 (299 aa). TBparse score is 0.912.; mpt59; 85B
  • DNA Sequence : Show Sequence
    >gi|57116681:2134889-2135866 Mycobacterium tuberculosis H37Rv, complete genome
    GTCAGCCGGCGCCTAACGAACTCTGCAGGTCACCCTTCATGGCGTTGAGCTGAGCGCCCCAGTACTCCCA
    GCTGTGCGTGCCGTTGGGCGGGAAGTTGAACACGGCGTTGTGCCCGCCCGCGGCGTTGTACGCATCCTGG
    AACTTCAGGTTGCTGCTACGAACGAAGTTCTCCAAGAACTCGGCGGGTATGTTGGCACCGCCCAACTCGT
    TCGGGGTGCCGTTCCCGCAATAAACCCATAGCCGGGTGTTGTTTGCGACCAGCTTGGGGATCTGCTGCGT
    AGGGTCGTTGCGCTCCCATGCCGGGTCACTCGAGGGACCCCACATGTCTGCGGCCTTGTAACCGCCGGCG
    TCACCCATCGCGAGGCCGATCAGGCTAGGCCCCATCCCCTGAGAGGGGTCCAGCAGGGCCGACAGCGAGC
    CGGCGTAGATGAACTGCTGGGGGTGGTAGGCGGCCAAGATCATTGCCGACGAGCCGGCCATCGACAAGCC
    GATTGCAGCGCTGCCGGTGGGCTTCACGGCCCTGTTGGCGGACAACCATTGCGGCAGCTCGCTGGTCAGG
    AAGGTTTCCCACTTGTAAGTCTGGCAGCCAGCCTTACCGCAGGCCGGGCTGTACCAGTCGCTGTAGAAGC
    TGGACTGCCCGCCGACCGGCATGACTATCGACAGTCCCGACTGGTAGTACCACTCGAACGCCGGGGTGTT
    GATATCCCAGCCGTTGTAGTCGTCTTGGGCGCGCAGGCCGTCGAGCAGATAAACCGCAGGTGAGTTGTTC
    CCACCGCTCTGGAACTGAACCTTGATGTCGCGGCCCATCGACGGCGACGGCACCTGCAGGTACTCGACCG
    GCAGCCCCGGCCGGGAGAACGCGCCCGCGGTTGCCGCTCCGCCGGCAAGCCCCACCAGGCCCGGAAGGAC
    TACAGCCGCTGCCGTGCCGATCATCAATCGGCGTCCCCAAGCTCGAATCTTTCGGCTCACGTCTGTCA
  • Protein Sequence : Show Sequence
    >gi|15609023|ref|NP_216402.1| secreted antigen 85-B fbpB (85B) (antigen 85 complex B) (Mycolyl transferase 85B) (fibronectin-binding protein B) (extracellular alpha-antigen) [Mycobacterium tuberculosis H37Rv]
    MTDVSRKIRAWGRRLMIGTAAAVVLPGLVGLAGGAATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQSGG
    NNSPAVYLLDGLRAQDDYNGWDINTPAFEWYYQSGLSIVMPVGGQSSFYSDWYSPACGKAGCQTYKWETF
    LTSELPQWLSANRAVKPTGSAAIGLSMAGSSAMILAAYHPQQFIYAGSLSALLDPSQGMGPSLIGLAMGD
    AGGYKAADMWGPSSDPAWERNDPTQQIPKLVANNTRLWVYCGNGTPNELGGANIPAEFLENFVRSSNLKF
    QDAYNAAGGHNAVFNFPPNGTHSWEYWGAQLNAMKGDLQSSLGAG
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Immunization with DNA encoding Ag85B (fbpB), a candidate vaccine antigen of Mycobacterium tuberculosis, elicited in mice a low but protective CD4(+) T cell-mediated IFN-gamma response (Palma et al., 2010).
  • Related Vaccine(s): M. tuberculosis DNA Vaccine encoding Ag85B Protein
13. FbpD
  • Gene Name : FbpD
  • Sequence Strain (Species/Organism) : Mycobacterium bovis BCG str. Tokyo 172
  • VO ID : VO_0012366
  • NCBI Gene ID : 7562680
  • NCBI Protein GI : 224992215
  • Locus Tag : JTY_3867
  • Genbank Accession : AP010918
  • Protein Accession : YP_002646905
  • 3D structure: PDB ID : 1R88
  • Taxonomy ID : 561275
  • Gene Starting Position : 4234158
  • Gene Ending Position : 4235057
  • Gene Strand (Orientation) : -
  • Protein Name : secreted MPT51/MPB51 antigen protein
  • Protein pI : 6.63
  • Protein Weight : 29500.66
  • Protein Length : 299
  • DNA Sequence : Show Sequence
    >gi|224988383:4234158-4235057 Mycobacterium bovis BCG str. Tokyo 172, complete genome
    CTTAGCGGATCGCACCGACGATATCGCCCGACATAGCGCCCAGCTGGGGCGCCCACGAGCCCCAGCCGTT
    GTCACCGCTGGCTGGGAAGTCGAAGTGTCCGTTGTGCCCGCCGACGCTGCGATACTGGTTGTAGAACATG
    CGGCTGTTACCCATCGCCTCGGCGGCTTGGCCGATCATGGCGGCGGGATCGCTGGCTCCCGGGTTGGTCG
    GGCTCCACACCCACACCCGGGTGTTGTTTTGCGCCAGCAGGCTGGCATGCACCCACGGGTCGTGCCACTT
    CCACCGACCCAGCTGTGGTGCTCCCCACATTCCGTTGGTGTCCACACCGCCGAATTGCTGCATGCCCGCC
    GCGATCGCACCGTTGGTGGTGGTGTTCGACGGGTACAAAAAGCCCGACATCGAGCCAGCGAAGCCGAAGC
    GGTCGGGGTGGAAGGCCGCCAGCGCCATCGCCCCGTAACCGCCCTGAGCGGCGCCAACGGCCGCATGGCC
    ACCGGGGGCCAAGCCCCGGTTAGCGGCCAGCCAGTCGGGCAGCTCAGCGGACAAGAAGGTGTCCCACTGC
    TTGCTGCCATCCTGCTCCCAGTTGGTGTACATGCTGTACGCACCACCGGCCGGTGCCACCACCGAAATCC
    CCTTGCCCGCCAACGTGTTCATCGCGTTACCCGCGGTGACCCAGTTACTGACATCCGGGCCGGCGTTGAA
    GGCGTCCAGCAGATACACCGCGTGCGGCCCACCGGCTAGGAAGGCCACCGGGATGTCCCGGCCCATCGAG
    GGCGACGGCACCATCAGGTTCTCGTATGGGGCGGCCTTGGCGGTGGGTTCCGCGGCTACCGCGACACCGC
    CCAACCCGAATGACAGTGCGGCAATCCAGAGCGCCCGCAGCAGCGCCGACCGACCCTTCA
  • Protein Sequence : Show Sequence
    >gi|224992215|ref|YP_002646905.1| secreted MPT51/MPB51 antigen protein [Mycobacterium bovis BCG str. Tokyo 172]
    MKGRSALLRALWIAALSFGLGGVAVAAEPTAKAAPYENLMVPSPSMGRDIPVAFLAGGPHAVYLLDAFNA
    GPDVSNWVTAGNAMNTLAGKGISVVAPAGGAYSMYTNWEQDGSKQWDTFLSAELPDWLAANRGLAPGGHA
    AVGAAQGGYGAMALAAFHPDRFGFAGSMSGFLYPSNTTTNGAIAAGMQQFGGVDTNGMWGAPQLGRWKWH
    DPWVHASLLAQNNTRVWVWSPTNPGASDPAAMIGQAAEAMGNSRMFYNQYRSVGGHNGHFDFPASGDNGW
    GSWAPQLGAMSGDIVGAIR
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Intravenous vaccination with recombinant bacteria carrying expression plasmid for MPB/MPT51 in BALB/c mice elicited significant protective responses, comparable to those evoked by a live Mycobacterium bovis BCG vaccine (Miki et al., 2004).
14. HBHA
  • Gene Name : HBHA
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Ra
  • VO ID : VO_0012373
  • NCBI Gene ID : 5211814
  • NCBI Protein GI : 148660242
  • Locus Tag : MRA_0482
  • Genbank Accession : CP000611
  • Protein Accession : YP_001281765
  • Taxonomy ID : 25177
  • Gene Starting Position : 567106
  • Gene Ending Position : 567705
  • Gene Strand (Orientation) : +
  • Protein Name : iron-regulated heparin binding hemagglutinin Hbha
  • Protein pI : 9.84
  • Protein Weight : 20541.79
  • Protein Length : 199
  • DNA Sequence : Show Sequence
    >gi|148659757:567106-567705 Mycobacterium tuberculosis H37Ra, complete genome
    CATGGCTGAAAACTCGAACATTGATGACATCAAGGCTCCGTTGCTTGCCGCGCTTGGAGCGGCCGACCTG
    GCCTTGGCCACTGTCAACGAGTTGATCACGAACCTGCGTGAGCGTGCGGAGGAGACTCGTACGGACACCC
    GCAGCCGGGTCGAGGAGAGCCGTGCTCGCCTGACCAAGCTGCAGGAAGATCTGCCCGAGCAGCTCACCGA
    GCTGCGTGAGAAGTTCACCGCCGAGGAGCTGCGTAAGGCCGCCGAGGGCTACCTCGAGGCCGCGACTAGC
    CGGTACAACGAGCTGGTCGAGCGCGGTGAGGCCGCTCTAGAGCGGCTGCGCAGCCAGCAGAGCTTCGAGG
    AAGTGTCGGCGCGCGCCGAAGGCTACGTGGACCAGGCGGTGGAGTTGACCCAGGAGGCGTTGGGTACGGT
    CGCATCGCAGACCCGCGCGGTCGGTGAGCGTGCCGCCAAGCTGGTCGGCATCGAGCTGCCTAAGAAGGCT
    GCTCCGGCCAAGAAGGCCGCTCCGGCCAAGAAGGCCGCTCCGGCCAAGAAGGCGGCGGCCAAGAAGGCGC
    CCGCGAAGAAGGCGGCGGCCAAGAAGGTCACCCAGAAGTA
  • Protein Sequence : Show Sequence
    >gi|148660242|ref|YP_001281765.1| iron-regulated heparin binding hemagglutinin Hbha [Mycobacterium tuberculosis H37Ra]
    MAENSNIDDIKAPLLAALGAADLALATVNELITNLRERAEETRTDTRSRVEESRARLTKLQEDLPEQLTE
    LREKFTAEELRKAAEGYLEAATSRYNELVERGEAALERLRSQQSFEEVSARAEGYVDQAVELTQEALGTV
    ASQTRAVGERAAKLVGIELPKKAAPAKKAAPAKKAAPAKKAAAKKAPAKKAAAKKVTQK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Purified preparations of HBHA were emulsified in a dimethyldioctadecylammonium bromide-monophosphoryl lipid A adjuvant and tested for the ability to reduce M. tuberculosis infection in the mouse aerosol challenge model for tuberculosis. The HBHA-containing vaccine gave a approximately 0.7-log reduction in CFU in both mouse lungs and spleens compared to adjuvant controls 28 days following challenge (Parra et al., 2004).
  • Related Vaccine(s): M. tuberculosis HBHA Protein Vaccine
15. KatG
  • Gene Name : KatG
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0012369
  • NCBI Gene ID : 885638
  • NCBI Protein GI : 15609045
  • Locus Tag : Rv1908c
  • Genbank Accession : BX842578
  • Protein Accession : NP_216424
  • 3D structure: PDB ID : 1SJ2
  • Taxonomy ID : 83332
  • Gene Starting Position : 2153888
  • Gene Ending Position : 2156110
  • Gene Strand (Orientation) : -
  • Protein Name : catalase-peroxidase-peroxynitritase T KATG
  • Protein pI : 4.87
  • Protein Weight : 75544.06
  • Protein Length : 740
  • Protein Note : Rv1908c, (MTCY180.10), len: 740 aa. katG, catalase-peroxidase-peroxynitritase T (EC 1.11.1.6) (see citations below), HPI. FASTA results: Q57215 CATALASE-PEROXIDASE from Mycobacterium tuberculosis (740 aa) opt: 5081, E(): 0, (100% identity in 740 aa overlap). Contains peroxidases active site signature (PS00436) and ATP/GTP-binding site motif A (P-loop; PS00017). Cosmid sequence was corrected to agree with a sequencing read from the H37Rv genome. DELETIONS OR DEFECTS IN KATG GENE CAUSE ISONIAZID (INH) RESISTANCE. BELONGS TO THE PEROXIDASE FAMILY. BACTERIAL PEROXIDASE/CATALASE SUBFAMILY. KATG TRANSCRIPTION SEEMS TO BE REGULATED BY FURA|Rv1909c PRODUCT. The catalase-peroxidase activity is associated with the amino-terminal domain but no definite function has been assigned to the carboxy-terminal domain. TBparse score is 0.923.
  • DNA Sequence : Show Sequence
    >gi|57116681:2153888-2156110 Mycobacterium tuberculosis H37Rv, complete genome
    ATCAGCGCACGTCGAACCTGTCGAGGTTCATCACCTTGTCCCAGGCAGCGACGAAGTCCTGCACGAACTT
    CGGCTGCGCGTCATCGGCGCCATAGACCTCGACAAGCGCCCGCAACTCCGAGTTGGACCCGAAGACCAGG
    TCCACGCGGCTGCCGGTCCACTTCACCTTGCCACTGCCATCCTTGCCCTGGTAGGTCCCGTCATCTGCTG
    GCGAGGGCTCCCAGGTGATACCCATGTCGAGCAGGTTCACGAAGAAGTCGTTGGTCAGTGACTCGGAGGC
    CTCGGTGAACACGCCCAGCGGTAAGCGCTTGTAGTTTGCGCCGAGGACGCGCAGGCCACCTACCAGCACC
    GTCATCTCAGGGGCACTGAGCGTAAGCAGGTTCGCCTTGTCGAGCAGCATGTACTCGGCCGGCAACGGGT
    TGCCCTTTCCGAGGTAGTTTCGGAAGCCATCTGCCTTGGGCTCCAGCACGGCAAAGGATTCCACGTCGGT
    TTGTTCCTGCGACGCATCCGTGCGGCCCGGGGTGAAGGGCACCGTGATGTTGTGGCCAGCCGCCTTTGCT
    GCTTTCTCTATGGCGGCACAGCCACCGAGCACGACGAGGTCGGCGAAGGACACTTTGATGTTCCCCGGCG
    CCGCGGAGTTGAATGACTCCTGGATCTCTTCCAGGGTGCGAATGACCTTGCGCAGATCCCCGTCGGGGTC
    GTTGACCTCCCACCCGACTTGTGGCTGCAGGCGGATGCGACCACCGTTGGCGCCGCCGCGCTTGTCGCTA
    CCACGGAACGACGACGCCGCCGCCCATGCGGTCGAAACTAGCTGTGAGACAGTCAATCCCGATGCCCGGA
    TCTGGCTCTTAAGGCTGGCAATCTCGGCTTCGCCGACGAGGTCGTGGCTGACCGCAGGGACCGGATCCTG
    CCACAGCAGGGTCTGCTTGGGGACCAGCGGCCCAAGGTATCTCGCAACGGGACCCATGTCTCGGTGGATC
    AGCTTGTACCAGGCCTTGGCGAACTCGTCGGCCAATTCCTCGGGGTGTTCCAGCCAGCGACGCGTGATCC
    GCTCATAGATCGGATCCACCCGCAGCGAGAGGTCAGTGGCCAGCATCGTCGGGGAGCGCCCTGGCCCGCC
    GAACGGGTCCGGGATGGTGCCGGCACCGGCGCCGTCCTTGGCGGTGTATTGCCAAGCGCCAGCAGGGCTC
    TTCGTCAGCTCCCACTCGTAGCCGTACAGGATCTCGAGGAAACTGTTGTCCCATTTCGTCGGGGTGTTCG
    TCCATACGACCTCGATGCCGCTGGTGATCGCGTCCTTACCGGTTCCGGTGCCATACGAGCTCTTCCAGCC
    CAAGCCCATCTGCTCCAGCGGAGCAGCCTCGGGTTCGGGGCCGACCAGATCGGCCGGGCCGGCGCCATGG
    GTCTTACCGAAAGTGTGACCGCCGACGATCAGCGCCGCTGTTTCGACGTCGTTCATGGCCATGCGCCGAA
    ACGTCTCGCGAATGTCGACCGCCGCGGCCATGGGGTCCGGGTTGCCGTTCGGCCCCTCCGGGTTCACGTA
    GATCAGCCCCATCTGCACCGCGGCCAGCGGGTTCTCCAGATCCCGCTTACCGCTGTAACGCTCATCGCCG
    AGCCAGGTGGCTTCCTTGCCCCAATAGACCTCATCGGGCTCCCACTGGTCGACCCGGCCGAAGCCGAACC
    CGAACGTCTTGAAGCCCATCGATTCCAGCGCGCAGTTGCCGGCGAAAACAATCAGGTCCGCCCATGAGAG
    CTTCTTGCCGTACTTCTTCTTGACCGGCCACAGCAGCCGGCGCGCCTTGTCCAAGCTGGCGTTGTCGGGC
    CAGCTGTTAAGCGGCGCGAACCGCTGCATGCCGCCCCCGGCGCCGCCGCGGCCGTCGTGGATGCGGTAGG
    TGCCGGCAGCGTGCCACGCCATCCGGATAAACAGCGGCCCGTAGTGGCCGTAGTCGGCGGGCCACCACGG
    CTGCGAGGTGGTCATCACTTCCTCGATGTCCCGCGTCAGGGCGTCAACGTCGATGGTCGCGACCTCCGCG
    GCATAGTCGAACGCCGCACCCATCGGGTCAGCGACGGCCGGGTTTTGGTGCAGTACCTTCAGATTGAGCC
    GGTTGGGCCACCAGTCCTGGTTTCCGCCGCCCTCGACGGGGTATTTCATATGACCCACGACGGGACAGCC
    GTTGCTAGCGGCTCCGGTGGTGGTTTCTGTAATGGGTGGGTGTTGCTCGGGCA
  • Protein Sequence : Show Sequence
    >gi|15609045|ref|NP_216424.1| catalase-peroxidase-peroxynitritase T KATG [Mycobacterium tuberculosis H37Rv]
    MPEQHPPITETTTGAASNGCPVVGHMKYPVEGGGNQDWWPNRLNLKVLHQNPAVADPMGAAFDYAAEVAT
    IDVDALTRDIEEVMTTSQPWWPADYGHYGPLFIRMAWHAAGTYRIHDGRGGAGGGMQRFAPLNSWPDNAS
    LDKARRLLWPVKKKYGKKLSWADLIVFAGNCALESMGFKTFGFGFGRVDQWEPDEVYWGKEATWLGDERY
    SGKRDLENPLAAVQMGLIYVNPEGPNGNPDPMAAAVDIRETFRRMAMNDVETAALIVGGHTFGKTHGAGP
    ADLVGPEPEAAPLEQMGLGWKSSYGTGTGKDAITSGIEVVWTNTPTKWDNSFLEILYGYEWELTKSPAGA
    WQYTAKDGAGAGTIPDPFGGPGRSPTMLATDLSLRVDPIYERITRRWLEHPEELADEFAKAWYKLIHRDM
    GPVARYLGPLVPKQTLLWQDPVPAVSHDLVGEAEIASLKSQIRASGLTVSQLVSTAWAAASSFRGSDKRG
    GANGGRIRLQPQVGWEVNDPDGDLRKVIRTLEEIQESFNSAAPGNIKVSFADLVVLGGCAAIEKAAKAAG
    HNITVPFTPGRTDASQEQTDVESFAVLEPKADGFRNYLGKGNPLPAEYMLLDKANLLTLSAPEMTVLVGG
    LRVLGANYKRLPLGVFTEASESLTNDFFVNLLDMGITWEPSPADDGTYQGKDGSGKVKWTGSRVDLVFGS
    NSELRALVEVYGADDAQPKFVQDFVAAWDKVMNLDRFDVR
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : DNA vaccines encoding KatG antigen fused to TPA signal sequences evoked a significant protective response in mice aerogenically challenged with low doses of Mycobacterium tuberculosis Erdman 17 to 21 days after the final immunization (Li et al., 1999).
  • Related Vaccine(s): M. tuberculosis DNA vaccine (containing the ESAT-6, MPT-64, MPT-83, and KatG constructs) , M. tuberculosis DNA Vaccine encoding KatG
16. leuD
  • Gene Name : leuD
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 888225
  • NCBI Protein GI : 15610124
  • Locus Tag : Rv2987c
  • Genbank Accession : BX842581
  • Protein Accession : NP_217503
  • 3D structure: PDB ID : 3H5J
  • Taxonomy ID : 83332
  • Gene Starting Position : 3344032
  • Gene Ending Position : 3344628
  • Gene Strand (Orientation) : -
  • Protein Name : isopropylmalate isomerase small subunit
  • Protein pI : 4.89
  • Protein Weight : 20390.6
  • Protein Length : 198
  • Protein Note : catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate in leucine biosynthesis; forms a heterodimer of LeuC/D
  • DNA Sequence : Show Sequence
    >gi|57116681:3344032-3344628 Mycobacterium tuberculosis H37Rv, complete genome
    CTCAGGGGGCGGGTAGAGTGCGCGGTTTCCAGTACGCGCACGCACCCTCAAAGGCCTCGATCTCGTCGAG
    TTTCCGCAGCGTAAGGGCTATATCGTCGAGACCTTCAAGCAGCCGCCACGCCGAGTGGTCGTCAATCTTG
    AACGGCAGCACCACTGTTGCTGCGGTGATAATTCGATCTTGAAGATTGGCAGTGATTTCCAGGCCCGGAC
    TCTGCTCAATGAGCTTCCACAGGAGTTCCACATCGTCTTGGGCAACCTCGGCCGCCAGCAGCCCGGCCTT
    GCCCGCGTTGCCGCGGAAAATGTCACCAAATCGGGATGAGATAACCACCCGGAATCCGTAGTCCATGAGC
    GCCCAGACCGCATGCTCTCGCGAGGATCCGGTGCCGAAATCGGGCCCGGCAACCAGGACCGAACCCCGGT
    CAAAGGGACTGAGGTTTAGCACGAATGCAGGATCCGACCGCCAACCCGCGAACAAGCCGTCCTCGAAACC
    GGTTCGGGTGACCCGCTTCAGAAAGACCGCGGGAATGATCTGATCGGTGTCGACATTGGACCGCCGCAAC
    GGCACGCCAATACCAGAGTGGGTGTGAAAGGCTTCCA
  • Protein Sequence : Show Sequence
    >gi|15610124|ref|NP_217503.1| isopropylmalate isomerase small subunit [Mycobacterium tuberculosis H37Rv]
    MEAFHTHSGIGVPLRRSNVDTDQIIPAVFLKRVTRTGFEDGLFAGWRSDPAFVLNLSPFDRGSVLVAGPD
    FGTGSSREHAVWALMDYGFRVVISSRFGDIFRGNAGKAGLLAAEVAQDDVELLWKLIEQSPGLEITANLQ
    DRIITAATVVLPFKIDDHSAWRLLEGLDDIALTLRKLDEIEAFEGACAYWKPRTLPAP
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Deletion of leuD gene was shown to be highly attenuated. Immunocompetent BALB/c mice vaccinated with the DleuD auxotroph were protected as well as BCG in terms of survival following an intravenous or aerosol challenge with virulent M. tuberculosis (Hondalus et al., 2000).
  • Related Vaccine(s): Mycobacterium tuberculosis leuD mutant vaccine
17. lysA
  • Gene Name : lysA
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 886960
  • NCBI Protein GI : 15608433
  • Locus Tag : Rv1293
  • Genbank Accession : BX842576
  • Protein Accession : NP_215809
  • 3D structure: PDB ID : 1HKW
  • Taxonomy ID : 83332
  • Gene Starting Position : 1448027
  • Gene Ending Position : 1449370
  • Gene Strand (Orientation) : ?
  • Protein Name : diaminopimelate decarboxylase LysA
  • Protein pI : 5.07
  • Protein Weight : 42101.78
  • Protein Length : 447
  • Protein Note : Rv1293, (MTCY373.13), len: 447 aa. Probable lysA, diaminopimelate decarboxylase (EC 4.1.1.20) (see citation below), almost identical to DCDA_MYCTU|P31848. Contains PS00878 Orn/DAP/Arg decarboxylases family 2 pyridoxal-P attachment site, PS00879 Orn/DAP/Arg decarboxylases family 2 signature 2. BELONGS TO FAMILY 2 OF ORNITHINE, DAP, AND ARGININE DECARBOXYLASES.
  • DNA Sequence : Show Sequence
    >gi|57116681:1448027-1449370 Mycobacterium tuberculosis H37Rv, complete genome
    TGTGAACGAGCTGCTGCACTTAGCGCCGAATGTGTGGCCGCGCAATACTACTCGCGATGAAGTCGGTGTG
    GTCTGCATCGCAGGAATTCCACTGACGCAGCTCGCCCAGGAGTACGGGACCCCGCTGTTCGTCATCGACG
    AGGACGACTTTCGCTCGCGCTGCCGAGAAACCGCCGCGGCCTTTGGAAGTGGGGCGAACGTGCACTATGC
    CGCCAAGGCGTTCCTGTGCAGCGAAGTAGCCCGGTGGATCAGCGAAGAAGGGCTCTGTCTGGACGTTTGC
    ACCGGTGGGGAGTTGGCGGTCGCGCTGCACGCTAGCTTTCCGCCCGAGCGAATTACCTTGCACGGCAACA
    ACAAATCGGTCTCAGAGTTGACCGCTGCGGTCAAAGCCGGAGTCGGCCATATTGTCGTCGATTCGATGAC
    CGAGATCGAGCGCCTCGACGCCATCGCGGGCGAGGCCGGAATCGTCCAGGATGTCCTGGTGCGTCTCACC
    GTCGGTGTCGAGGCGCACACCCACGAGTTCATCTCCACCGCGCACGAGGACCAGAAATTCGGGTTATCGG
    TGGCCAGCGGCGCGGCCATGGCAGCGGTGCGGCGCGTTTTCGCCACTGATCACCTGCGCCTGGTTGGGCT
    ACACAGCCACATCGGTTCGCAGATCTTCGACGTGGACGGCTTCGAACTCGCCGCGCACCGTGTCATCGGC
    CTGCTACGCGACGTCGTCGGCGAGTTCGGTCCCGAAAAGACGGCACAGATCGCGACCGTCGATCTCGGTG
    GCGGCTTGGGCATCTCGTATTTGCCGTCCGACGACCCACCGCCGATAGCCGAGCTCGCGGCCAAGCTGGG
    TACCATCGTGAGCGACGAGTCAACGGCCGTGGGGCTGCCGACGCCCAAGCTCGTTGTGGAGCCCGGACGC
    GCCATCGCCGGACCGGGCACCATCACGTTGTATGAGGTCGGCACCGTTAAGGACGTCGATGTCAGCGCCA
    CAGCGCATCGACGTTACGTCAGTGTCGACGGCGGCATGAGCGACAACATCCGCACCGCGCTCTACGGCGC
    GCAGTATGACGTCCGGCTGGTGTCTCGAGTCAGCGACGCCCCGCCGGTACCGGCCCGTCTGGTCGGAAAG
    CACTGCGAAAGTGGCGATATCATCGTGCGGGACACCTGGGTGCCCGACGATATTCGGCCCGGCGATCTGG
    TTGCGGTTGCCGCCACCGGCGCTTACTGCTATTCGCTGTCGAGTCGTTACAACATGGTCGGCCGTCCCGC
    TGTGGTAGCGGTGCACGCGGGCAACGCTCGCCTGGTCCTGCGTCGGGAGACGGTCGACGATTTGCTGAGT
    TTGGAAGTGAGGTG
  • Protein Sequence : Show Sequence
    >gi|15608433|ref|NP_215809.1| diaminopimelate decarboxylase LysA [Mycobacterium tuberculosis H37Rv]
    MNELLHLAPNVWPRNTTRDEVGVVCIAGIPLTQLAQEYGTPLFVIDEDDFRSRCRETAAAFGSGANVHYA
    AKAFLCSEVARWISEEGLCLDVCTGGELAVALHASFPPERITLHGNNKSVSELTAAVKAGVGHIVVDSMT
    EIERLDAIAGEAGIVQDVLVRLTVGVEAHTHEFISTAHEDQKFGLSVASGAAMAAVRRVFATDHLRLVGL
    HSHIGSQIFDVDGFELAAHRVIGLLRDVVGEFGPEKTAQIATVDLGGGLGISYLPSDDPPPIAELAAKLG
    TIVSDESTAVGLPTPKLVVEPGRAIAGPGTITLYEVGTVKDVDVSATAHRRYVSVDGGMSDNIRTALYGA
    QYDVRLVSRVSDAPPVPARLVGKHCESGDIIVRDTWVPDDIRPGDLVAVAATGAYCYSLSSRYNMVGRPA
    VVAVHAGNARLVLRRETVDDLLSLEVR
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A lysA mutant, in combination with a secA2 mutation, is highly attenuated in mice and induced significant protection from challenge with wild type M. tuberculosis (Hinchey et al., 2011).
  • Related Vaccine(s): Mycobacterium tuberculosis lysA/secA2 mutant vaccine
18. mce-2
  • Gene Name : mce-2
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis CDC1551
  • NCBI Gene ID : 925014
  • NCBI Protein GI : 15839991
  • Locus Tag : MT0618
  • Genbank Accession : AE000516
  • Protein Accession : NP_335028
  • Taxonomy ID : 83331
  • Gene Starting Position : 688269
  • Gene Ending Position : 689483
  • Gene Strand (Orientation) : +
  • Protein Name : virulence factor
  • Protein pI : 4.96
  • Protein Weight : 39757.76
  • Protein Length : 404
  • Protein Note : identified by similarity to GP:5814111; match to protein family HMM PF02470; match to protein family HMM TIGR00996
  • DNA Sequence : Show Sequence
    >gi|50953765:688269-689483 Mycobacterium tuberculosis CDC1551, complete genome
    GGTGCCAACGCTGGTGACGAGGAAGAACCGACGTGCGTGGCTGTATGTGGAGGGTGTTGTCCTGCTGTTG
    GTGGGCGCGTTGGTGCTCGTATTGGTGTACAAGCAGTTTCGTGGGGAATTCACGCCGAAGACCGAGCTGA
    CTATGGTCGCCTCCCGGGCTGGGCTGGTTATGGAAGCTGGATCCAAAGTCACCTACAACGGGGTGGAGAT
    CGGCCGGGTGGGCAGCATTTCGGAGATTGAGCGTGACGGCCGGCCGGCGGCGAAGCTGGTTTTGGACGTG
    AATCCTCGCTACATCAGCCTGATTCCGGTCAATGTGGTGGCCGATATCGAGGCGGCCACCCTGTTCGGCA
    ACAAGTATGTTGCGCTGTCCGCGCCGAAAATTCCTCAACAGCAGCGGATTTCCTCACATGACGTGATTGA
    TGTGGGGTCGGTGACCACCGAATTCAACACGTTGTTCGAGACGATCACCTCGATCGCCGAGAAGGTGGAT
    CCGATCGAGCTGAACGCGACGCTGTCCGCGGTAGCACAGGCGCTGGATGGGCTGGGCGGCAAGTTCGGTG
    AGTCGATCGTTAATGGCAATCAGATTCTGGCGCAATTAAATCCGCGGCTGCCGCAGCTCGGCTATGATGT
    TCGGCGGTTGGCGGATCTCGGTGAGGTCTATGTCGATGCTTCGCCGGATCTGTGGTCCTTTCTGCAGAAC
    GCACTGACCACTGCGCGCACATTGACCAGCCAACAGCGCGATCTGGATGCCGCGTTGTTGGCGGCTACGG
    GTGCGGGCAACACCGGTGAAGACGTTTTTGCTCGAGGCGGGCCGTATCTTGCGCGCGCAGCCGCCGATCT
    GGTGCCCACCGCTACGCTGCTGGACACCTACAGTCCCGAACTGTTCTGCATGATCCGCAACTTTCACGAC
    GCTGCGCCCAAAGTCGCGGACGCGGTGGGCGGCAACGGCTATTCGCTAGCGGCCGCCGGAACGATTTTGG
    GAGCACCCAATCCCTATGTCTATCCGGACAATCTGCCGCGGGTGAATGCCCACGGTGGACCCGGGGGCCG
    ACCGGGCTGCTGGCAGACGATCACCCGGGAGCTGTGGCCGGCACCCTATCTGGTGATGGACACCGGTGCC
    AGCCTCGCACCGTACAACCACGTCGAGCTCGGCCAACCGATGTTCACTGAATACGTATGGGGACGCCAAT
    ACGGAGAGAACACGATCAACCCATG
  • Protein Sequence : Show Sequence
    >gi|15839991|ref|NP_335028.1| virulence factor [Mycobacterium tuberculosis CDC1551]
    MPTLVTRKNRRAWLYVEGVVLLLVGALVLVLVYKQFRGEFTPKTELTMVASRAGLVMEAGSKVTYNGVEI
    GRVGSISEIERDGRPAAKLVLDVNPRYISLIPVNVVADIEAATLFGNKYVALSAPKIPQQQRISSHDVID
    VGSVTTEFNTLFETITSIAEKVDPIELNATLSAVAQALDGLGGKFGESIVNGNQILAQLNPRLPQLGYDV
    RRLADLGEVYVDASPDLWSFLQNALTTARTLTSQQRDLDAALLAATGAGNTGEDVFARGGPYLARAAADL
    VPTATLLDTYSPELFCMIRNFHDAAPKVADAVGGNGYSLAAAGTILGAPNPYVYPDNLPRVNAHGGPGGR
    PGCWQTITRELWPAPYLVMDTGASLAPYNHVELGQPMFTEYVWGRQYGENTINP
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Mce-2 and mce-3 mutants in Mycobacterium tuberculosis is attenuated and provide protection against challenge for mice (Aguilar et al., 2006).
  • Related Vaccine(s): Mycobacterium tuberculosis mce-2 mutant vaccine
19. mce-3
  • Gene Name : mce-3
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis CDC1551
  • NCBI Gene ID : 923491
  • NCBI Protein GI : 15841441
  • Locus Tag : MT2018
  • Genbank Accession : AE000516
  • Protein Accession : NP_336478
  • Taxonomy ID : 83331
  • Gene Starting Position : 2206659
  • Gene Ending Position : 2207936
  • Gene Strand (Orientation) : +
  • Protein Name : virulence factor
  • Protein pI : 6.51
  • Protein Weight : 41527.31
  • Protein Length : 425
  • Protein Note : identified by similarity to GP:5814111; match to protein family HMM PF02470; match to protein family HMM TIGR00996
  • DNA Sequence : Show Sequence
    >gi|50953765:2206659-2207936 Mycobacterium tuberculosis CDC1551, complete genome
    CATGAGACGCGGGCCGGGTCGACACCGTTTGCACGACGCGTGGTGGACGCTGATCCTGTTCGCGGTGATC
    GGGGTGGCTGTCCTGGTGACGGCGGTGTCCTTCACGGGCAGCTTGCGGTCGACTGTGCCGGTGACGCTGG
    CGGCCGACCGCTCCGGGCTGGTGATGGACTCCGGCGCCAAGGTCATGATGCGCGGTGTGCAGGTCGGCCG
    GGTCGCCCAGATCGGTCGGATCGAGTGGGCCCAGAACGGGGCGAGCCTCAGACTGGAGATCGACCCCGAC
    CAGATCCGGTACATCCCGGCCAATGTCGAGGCACAGATCAGCGCCACCACCGCATTCGGTGCCAAGTTCG
    TCGACCTGGTGATGCCGCAAAACCCAAGTCGTGCACGGCTGTCCGCTGGGGCGGTACTGCATTCGAAGAA
    CGTCAGCACGGAAATCAACACCGTCTTCGAAAACGTCGTCGACCTGCTCAACATGATCGACCCGCTGAAA
    CTGAACGCCGTGCTGACCGCGGTCGCCGACGCCGTTCGCGGGCAAGGTGAACGGATAGGCCAGGCCACCA
    CCGACCTCAACGAGGTGCTGGAGGCACTCAACGCACGCGGCGACACCATCGGCGGCAACTGGCGATCGCT
    CAAGAACTTCACCGACACCTATGACGCGGCCGCCCAAGACATCCTGACGATCCTGAACGCCGCCAGCACC
    ACCAGTGCGACCGTCGTGAATCATTCGACGCAGCTGGATGCCTTGCTACTCAACGCCATCGGACTATCCA
    ACGCTGGCACCAACCTGCTTGGCAGCAGCCGAGACAATCTCGTCGGCGCGGCCGACATCCTGGCGCCGAC
    CACGAGCCTGCTGTTCAAGTACAACCCCGAATACACCTGCTTCCTGCAGGGCGCCAAGTGGTATCTCGAC
    AACGGCGGCTATGCGGCCTGGGGCGGGGCCGACGGGCGCACGCTACAACTCGATGTGGCGCTACTGTTCG
    GCAACGACCCCTATGTCTATCCGGACAACCTGCCGGTTGTCGCGGCCAAGGGGGGTCCCGGCGGAAGGCC
    GGGATGCGGGCCATTGCCGGATGCCACCCACAACTTCCCGGTGCGCCAGCTGGTCACCAACACCGGATGG
    GGAACCGGGCTGGACATCCGGCCCAACCCCGGCATCGGGCATCCCTGCTGGGCCAACTACTTCCCGGTGA
    CCCGCGCGGTGCCCGAGCCGCCGTCGATCCGTCAGTGCATCCCCGGGCCGGCGATCGGGCCCAACCCCGC
    GGCGGGGGAGCAGCCATG
  • Protein Sequence : Show Sequence
    >gi|15841441|ref|NP_336478.1| virulence factor [Mycobacterium tuberculosis CDC1551]
    MRRGPGRHRLHDAWWTLILFAVIGVAVLVTAVSFTGSLRSTVPVTLAADRSGLVMDSGAKVMMRGVQVGR
    VAQIGRIEWAQNGASLRLEIDPDQIRYIPANVEAQISATTAFGAKFVDLVMPQNPSRARLSAGAVLHSKN
    VSTEINTVFENVVDLLNMIDPLKLNAVLTAVADAVRGQGERIGQATTDLNEVLEALNARGDTIGGNWRSL
    KNFTDTYDAAAQDILTILNAASTTSATVVNHSTQLDALLLNAIGLSNAGTNLLGSSRDNLVGAADILAPT
    TSLLFKYNPEYTCFLQGAKWYLDNGGYAAWGGADGRTLQLDVALLFGNDPYVYPDNLPVVAAKGGPGGRP
    GCGPLPDATHNFPVRQLVTNTGWGTGLDIRPNPGIGHPCWANYFPVTRAVPEPPSIRQCIPGPAIGPNPA
    AGEQP
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Mce-2 and mce-3 mutants in Mycobacterium tuberculosis is attenuated and provide protection against challenge for mice (Aguilar et al., 2006).
  • Related Vaccine(s): Mycobacterium tuberculosis mce-2 mutant vaccine , Mycobacterium tuberculosis mce-3 mutant vaccine
20. Mpt63
  • Gene Name : Mpt63
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0011176
  • NCBI Gene ID : 885334
  • NCBI Protein GI : 15609063
  • Locus Tag : Rv1926c
  • Genbank Accession : U27119
  • Protein Accession : NP_216442
  • 3D structure: PDB ID : 1LMI
  • Taxonomy ID : 83332
  • Gene Starting Position : 2178956
  • Gene Ending Position : 2179435
  • Gene Strand (Orientation) : -
  • Protein Name : immunogenic protein MPT63 (antigen MPT63/MPB63) (16 kDa immunoprotective extracellular protein)
  • Protein pI : 4.67
  • Protein Weight : 14827.76
  • Protein Length : 159
  • Protein Note : Rv1926c, (MT1977, MTCY09F9.38), len: 159 aa. mpt63 (alternate gene name: mpb63), immunogenic protein (see citations below), identical to MPT63|MPB63 from Mycobacterium bovis (159 aa). Exported protein containing a N-terminal signal sequence: see notes below about proteomics.; mpb63
  • DNA Sequence : Show Sequence
    >gi|57116681:2178956-2179435 Mycobacterium tuberculosis H37Rv, complete genome
    TCTACGGCTCCCAAATCAGCAGATCCTCCATGCCGTTGTTCATCGCGACGATGGTTGGCGATGGGCCGGT
    GACATCGAAGTAGATTTTGCCGGTCGATTGTTCGCCTTGGGGGATAGTGGCTCCGCTAATGGTGTCGGGG
    CCCGCGGCTTGCCACAGCACCCGGTAGTTGATGCCGTCGGCGGTGCGGGCATTGAACTGCGAGACCGCGG
    GCGTGACGCTGCCGCGAATCGCATTGACCGTGGCAGTGGCCTCCCAGACCTGGCCGGCCACCGGATAGCC
    GGGGATGACTGCCGTGCTGGATTTGAGATCACTGACCTTCCAGCCGAGCACGACTTGGCCAACGGTGTCG
    GTCATCGTTAGCTCACTGCCAAGTTTTCCGGTGATGGGATAGGCAGCCAACGCGACCGGTGCCGCAAAGG
    TCGCGATGGCCGCCATGGCCACGACCGCTACTGCCGTCTTGATCATTGTGGTGAGCTTCA
  • Protein Sequence : Show Sequence
    >gi|15609063|ref|NP_216442.1| immunogenic protein MPT63 (antigen MPT63/MPB63) (16 kDa immunoprotective extracellular protein) [Mycobacterium tuberculosis H37Rv]
    MKLTTMIKTAVAVVAMAAIATFAAPVALAAYPITGKLGSELTMTDTVGQVVLGWKVSDLKSSTAVIPGYP
    VAGQVWEATATVNAIRGSVTPAVSQFNARTADGINYRVLWQAAGPDTISGATIPQGEQSTGKIYFDVTGP
    SPTIVAMNNGMEDLLIWEP
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : MPT63 (Rv1926c), a major secreted protein of Mycobacterium tuberculosis, is immunoreactive in antibody assays in humans and animals and provides protection as a combined DNA vaccine in mice (Mustafa, 2009).
21. MPT64
  • Gene Name : MPT64
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0012367
  • NCBI Gene ID : 885925
  • NCBI Protein GI : 15609117
  • Locus Tag : Rv1980c
  • Genbank Accession : A42312
  • Protein Accession : NP_216496
  • 3D structure: PDB ID : 2HHI
  • Taxonomy ID : 83332
  • Gene Starting Position : 2223342
  • Gene Ending Position : 2224028
  • Gene Strand (Orientation) : -
  • Protein Name : immunogenic protein MPT64 (antigen MPT64/MPB64)
  • Protein pI : 4.6
  • Protein Weight : 23267.4
  • Protein Length : 228
  • Protein Note : Rv1980c, (MT2032, MTCY39.39), len: 228 aa. mpt64 (alternate gene name: mpb64), immunogenic protein (alternate gene name: mpb64) (see citations below), identical to MPT64|MPB64 from Mycobacterium bovis (228 aa). Similar to Rv3036c|MTV012.51c from Mycobacterium tuberculosis. Exported protein containing a N-terminal signal sequence: see notes below about proteomics.; mpb64
  • DNA Sequence : Show Sequence
    >gi|57116681:2223342-2224028 Mycobacterium tuberculosis H37Rv, complete genome
    TCTAGGCCAGCATCGAGTCGATCGCGGAACGTGGGACCAATACCTGGGTTGGGCCGGCTGCTTCGGGCAG
    CAACTCCCCCGGGTTGAAGAAGAAAATCACCCCGTCGTTCGTGACTGCGAAGTTCTGATAATTCACCGGG
    TCCAAGCCGGCATTCGGCGCTATCGATACCTGTTGTCCGGTCTGCTTGCTCAGTTCACCTTGCACAATGG
    GGAAGACGACTGGCAGCGGATCGGTGTCAGCCTGCCACAGCGTGTCATAGGTGATTGGCTTGCGATAGGC
    CTGGTCCCAATCGAAGGCCTTGTACGTGGTCGTTGGGTGCGTGCCGCCGGCGTTCTGGTAGACCTTGAGC
    ACCACGGCCTGCGTACCACGCGGCGGTATCGCGGACTGGTATGTGGCCGAGGTGATATTCAATTCGTAGG
    GGGCTTCGCGTGGAGTGGACGATGTGGCCGCGCTGAGGAACTTGTCGCGCGTCTGGGCGATGTAATTTTC
    CAGCGACTTCTGGTCGGGGTAGTAACTGGGCAGGCTGATGTTGATGTTGTAGGCCGGGTCGGACATTTGA
    ATCTGGCACGCCTGGCCGGTATCGGTGCCTTTCAACTCCTCGCAGTAGGTCTTGGGCGCGGCCGTGGCCA
    CACCCGAACAACAGAGCAAAACGACAGCCGTGACCAGCATGAAGATCTTGATGCGCA
  • Protein Sequence : Show Sequence
    >gi|15609117|ref|NP_216496.1| immunogenic protein MPT64 (antigen MPT64/MPB64) [Mycobacterium tuberculosis H37Rv]
    MRIKIFMLVTAVVLLCCSGVATAAPKTYCEELKGTDTGQACQIQMSDPAYNINISLPSYYPDQKSLENYI
    AQTRDKFLSAATSSTPREAPYELNITSATYQSAIPPRGTQAVVLKVYQNAGGTHPTTTYKAFDWDQAYRK
    PITYDTLWQADTDPLPVVFPIVQGELSKQTGQQVSIAPNAGLDPVNYQNFAVTNDGVIFFFNPGELLPEA
    AGPTQVLVPRSAIDSMLA
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : DNA vaccines encoding secreted proteins Ag85B and MPT64 protect mice from the tuberculosis H37Rv strain (Luo et al., 2004).
    *Note* MPT64 in combination with Ag85B was protective, DNA vaccine of just MPT64 was mildly protective in mice.
  • Related Vaccine(s): M. tuberculosis DNA vaccine (containing the ESAT-6, MPT-64, MPT-83, and KatG constructs) , M. tuberculosis DNA vaccine encoding Ag85B, MPT64 and MPT83
22. MPT83
  • Gene Name : MPT83
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0012409
  • NCBI Gene ID : 887155
  • NCBI Protein GI : 15610010
  • Locus Tag : Rv2873
  • Genbank Accession : BX842581
  • Protein Accession : NP_217389
  • Taxonomy ID : 83332
  • Gene Starting Position : 3183904
  • Gene Ending Position : 3184566
  • Gene Strand (Orientation) : ?
  • Protein Name : cell surface lipoprotein mpt83 (lipoprotein P23)
  • Protein pI : 4.73
  • Protein Weight : 20581.44
  • Protein Length : 220
  • Protein Note : Rv2873, (MTCY274.04), len: 220 aa. mpt83 (alternate gene name: mpb83), cell surface lipoprotein (see citations below). Also similar to upstream ORF Q50769|MP70_MYCTU|MPT70|MPB70|Rv2875|MT2943|MTCY274.06 which is also known as MAJOR SECRETED IMMUNOGENIC PROTEIN MPT70 PRECURSOR from Mycobacterium tuberculosis (193 aa), FASTA scores: opt: 806, E(): 2.7e-38, (70.25% identity in 185 aa overlap). BELONGS TO THE MPT70 / MPT83 FAMILY. ATTACHED TO THE MEMBRANE BY A LIPID ANCHOR.; mpb83
  • DNA Sequence : Show Sequence
    >gi|57116681:3183904-3184566 Mycobacterium tuberculosis H37Rv, complete genome
    GATGATCAACGTTCAGGCCAAACCGGCCGCAGCAGCGAGCCTCGCAGCCATCGCGATTGCGTTCTTAGCG
    GGTTGTTCGAGCACCAAACCCGTGTCGCAAGACACCAGCCCGAAACCGGCGACCAGCCCGGCGGCGCCCG
    TTACCACGGCGGCAATGGCTGACCCCGCAGCGGACCTGATTGGTCGTGGGTGCGCGCAATACGCGGCGCA
    AAATCCCACCGGTCCCGGATCGGTGGCCGGAATGGCGCAAGACCCGGTCGCTACCGCGGCTTCCAACAAC
    CCGATGCTCAGTACCCTGACCTCGGCTCTGTCGGGCAAGCTGAACCCGGATGTGAATCTGGTCGACACCC
    TCAACGGCGGCGAGTACACCGTTTTCGCCCCCACCAACGCCGCATTCGACAAGCTGCCGGCGGCCACTAT
    CGATCAACTCAAGACTGACGCCAAGCTGCTCAGCAGCATCCTGACCTACCACGTGATAGCCGGCCAGGCG
    AGTCCGAGCAGGATCGACGGCACCCATCAGACCCTGCAAGGTGCCGACCTGACGGTGATAGGCGCCCGCG
    ACGACCTCATGGTCAACAACGCCGGTTTGGTATGTGGCGGAGTTCACACCGCCAACGCGACGGTGTACAT
    GATCGATACGGTGCTGATGCCCCCGGCACAGTA
  • Protein Sequence : Show Sequence
    >gi|15610010|ref|NP_217389.1| cell surface lipoprotein mpt83 (lipoprotein P23) [Mycobacterium tuberculosis H37Rv]
    MINVQAKPAAAASLAAIAIAFLAGCSSTKPVSQDTSPKPATSPAAPVTTAAMADPAADLIGRGCAQYAAQ
    NPTGPGSVAGMAQDPVATAASNNPMLSTLTSALSGKLNPDVNLVDTLNGGEYTVFAPTNAAFDKLPAATI
    DQLKTDAKLLSSILTYHVIAGQASPSRIDGTHQTLQGADLTVIGARDDLMVNNAGLVCGGVHTANATVYM
    IDTVLMPPAQ
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : A single DNA vaccine encoding the MPT-83 tuberculosis antigens evoked partial protection against an aerogenic challenge with M. tuberculosis Erdman in the mouse model of pulmonary tuberculosis (Morris et al., 2000).
  • Related Vaccine(s): M. tuberculosis DNA vaccine (containing the ESAT-6, MPT-64, MPT-83, and KatG constructs) , M. tuberculosis DNA vaccine encoding Ag85B, MPT64 and MPT83
23. Mtb72F
  • Gene Name : Mtb72F
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis
  • VO ID : VO_0011173
  • NCBI Protein GI : 283801990
  • Taxonomy ID : 32630
  • Gene Strand (Orientation) : ?
  • Protein Length : 729
  • Protein Note : Description of Artificial Sequence:tri-fusion protein MTB72F (Ra12-TbH9-Ra35 or MTB32-MTB39 fusion)
  • Protein Sequence : Show Sequence
    >gi|283801990|emb|CBI85484.1| unnamed protein product [synthetic construct]
    MHHHHHHTAASDNFQLSQGGQGFAIPIGQAMAIAGQIRSGGGSPTVHIGPTAFLGLGVVDNNGNGARVQR
    VVGSAPAASLGISTGDVITAVDGAPINSATAMADALNGHHPGDVISVTWQTKSGGTRTGNVTLAEGPPAE
    FMVDFGALPPEINSARMYAGPGSASLVAAAQMWDSVASDLFSAASAFQSVVWGLTVGSWIGSSAGLMVAA
    ASPYVAWMSVTAGQAELTAAQVRVAAAAYETAYGLTVPPPVIAENRAELMILIATNLLGQNTPAIAVNEA
    EYGEMWAQDAAAMFGYAAATATATATLLPFEEAPEMTSAGGLLEQAAAVEEASDTAAANQLMNNVPQALQ
    QLAQPTQGTTPSSKLGGLWKTVSPHRSPISNMVSMANNHMSMTNSGVSMTNTLSSMLKGFAPAAARQAVQ
    TAAQNGVRAMSSLGSSLGSSGLGGGVAANLGRAASVGSLSVPQAWAAANQAVTPAARALPLTSLTSAAER
    GPGQMLGGLPVGQMGARAGGGLSGVLRVPPRPYVMPHSPAAGDIAPPALSQDRFADFPALPLDPSAMVAQ
    VGPQVVNINTKLGYNNAVGAGTGIVIDPNGVVLTNNHVIAGATDINAFSVGSGQTYGVDVVGYDRTQDVA
    VLQLRGAGGLPSAAIGGGVAVGEPVVAMGNSGGQGGTPRAVPGRVVALGQTVQASDSLTGAEETLNGLIQ
    FDAAIQPGDSGGPVVNGLGQVVGMNTAAS
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Our lead construct, Mtb72F, codes for a 72-kDa polyprotein genetically linked in tandem in the linear order Mtb32(C)-Mtb39-Mtb32(N). All forms of Mtb72F immunization tested resulted in the protection of C57BL/6 mice against aerosol challenge with a virulent strain of M. tuberculosis (Skeiky et al., 2004).
  • Related Vaccine(s): M. tuberculosis Mtb72F Protein Subunit Vaccine
24. panC
  • Gene Name : panC
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 885459
  • NCBI Protein GI : 15610738
  • Locus Tag : Rv3602c
  • Genbank Accession : BX842583
  • Protein Accession : NP_218119
  • Taxonomy ID : 83332
  • Gene Starting Position : 4044280
  • Gene Ending Position : 4045209
  • Gene Strand (Orientation) : -
  • Protein Name : pantoate--beta-alanine ligase
  • Protein pI : 5.96
  • Protein Weight : 29602.42
  • Protein Length : 309
  • Protein Note : catalyzes the formation of (R)-pantothenate from pantoate and beta-alanine
  • DNA Sequence : Show Sequence
    >gi|57116681:4044280-4045209 Mycobacterium tuberculosis H37Rv, complete genome
    ATCAGTTTCTCCAATGTGATTCGAGGATTGCCCGGTATCCGTCCGGGCGGTCGGTGCCGGCGAAAGTTCC
    GATTTCAATCGCAATGTTGTCCAGCAGCCTGGTGGTGCCAAGCCGGGCAGCAACCAGCAGCCGACCGGAA
    CCGTTGAGCGGCATCGGGCCAAGCCCGATATCGCGCAGCTCCAGGTAGTCGACCGCCACGCCGGGTGCAG
    CGTCGAGCACCGCACGGGCGGCATCCAGCGCGGCCTGCGCGCCAGCCGTTGCCGCATGCGCTGCGGCCGT
    TAGCGCCGCCGAGAGCGCGACGGCCGCCGCACGCTGGGCCGGGTCCAGGTAGCGGTTGCGCGACGACATC
    GCCAGCCCGTCGGCTTCGCGCACGGTCGGCACGCCGACCACCGCGACATCGAGGTTGAAGTCCGCGACCA
    GCTGCCGGATCAGCACCAGCTGCTGGTAGTCCTTCTCACCGAAGAACACCCGATCCGGGCGCACGATCTG
    CAGCAGCTTTAGCACGACCGTCAGCACGCCGGCGAAATGGGTTGGCCGCGGGCCGCCCTCGAGTTCGGCG
    GCCAACGGACCGGGTTGCACGGTGGTGCGCAGGCCGTCGGGATACATCGCCGCGGTAGTTGGCGTGAAAG
    CGATTTCCACGCCTTCGGCCCGCAGTTGCGCCAGGTCGTCGTCCGGGGTGCGGGGATAGGCGTCGAGATC
    TTCCCCGGCACCGAATTGCATCGGGTTGACGAAGATCGACACGACGACGACCGATCCGGGCACCCGCTTG
    GCCGCACGCACCAACGCGAGGTGGCCTTCGTGCAGCGCACCCATAGTAGGCACCAACATCACTCGCCGGC
    CGGTGAGTCGCAGTGCGCGACTGACATCGGCGACATCCCCCGGTGCCGAGTACACATTGAGTTCACCGGG
    ATGGAACGCAGGAATCGTCA
  • Protein Sequence : Show Sequence
    >gi|15610738|ref|NP_218119.1| pantoate--beta-alanine ligase [Mycobacterium tuberculosis H37Rv]
    MTIPAFHPGELNVYSAPGDVADVSRALRLTGRRVMLVPTMGALHEGHLALVRAAKRVPGSVVVVSIFVNP
    MQFGAGEDLDAYPRTPDDDLAQLRAEGVEIAFTPTTAAMYPDGLRTTVQPGPLAAELEGGPRPTHFAGVL
    TVVLKLLQIVRPDRVFFGEKDYQQLVLIRQLVADFNLDVAVVGVPTVREADGLAMSSRNRYLDPAQRAAA
    VALSAALTAAAHAATAGAQAALDAARAVLDAAPGVAVDYLELRDIGLGPMPLNGSGRLLVAARLGTTRLL
    DNIAIEIGTFAGTDRPDGYRAILESHWRN
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A panCD mutant is attenuated in mice and induces significant protection from challenge with wild type M. tuberculosis (Sambandamurthy et al., 2002).
  • Related Vaccine(s): Mycobacterium tuberculosis panCD mutant vaccine , Mycobacterium tuberculosis RD1/panCD mutant vaccine
25. panD
  • Gene Name : panD
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 885596
  • NCBI Protein GI : 15610737
  • Locus Tag : Rv3601c
  • Genbank Accession : BX842583
  • Protein Accession : NP_218118
  • Taxonomy ID : 83332
  • Gene Starting Position : 4043861
  • Gene Ending Position : 4044280
  • Gene Strand (Orientation) : -
  • Protein Name : aspartate alpha-decarboxylase
  • Protein pI : 4.69
  • Protein Weight : 13595.37
  • Protein Length : 139
  • Protein Note : Converts L-aspartate to beta-alanine and provides the major route of beta-alanine production in bacteria. Beta-alanine is essential for the biosynthesis of pantothenate (vitamin B5)
  • DNA Sequence : Show Sequence
    >gi|57116681:4043861-4044280 Mycobacterium tuberculosis H37Rv, complete genome
    GCTATCCCACACCGAGCCGGGGGTCTAGCAGCTCGCCCGCGTTTTCGGGCACAAATGCCGGATCGTGGCC
    CATGTCGATCGGTTTGTTGTAAGCGTCGACAAACACGATCCGCGGCTGGTATGTGCGGGCCCGGGCGTCG
    TCCATCGTCGCGTACGCAATCAGAATCACCAGATCCCCCGGATGCACCAAGTGCGCGGCGGCACCGTTGA
    TGCCAATCACACCACTGCCGCGTTCGCCGGTGATCGCGTAGGTGACCAGTCGAGCACCGTTGTCGATATC
    GACGATGGTTACCTGTTCGCCTTCCAGCAGGTCGGCGGCGTCCATCAAGTCGGCATCGATGGTCACCGAG
    CCGACGTAGTGCAGGTCGGCGCAGGTCACCGTGGCGCGGTGGATCTTCGACTTCAGCATCGTCCGTAACA
  • Protein Sequence : Show Sequence
    >gi|15610737|ref|NP_218118.1| aspartate alpha-decarboxylase [Mycobacterium tuberculosis H37Rv]
    MLRTMLKSKIHRATVTCADLHYVGSVTIDADLMDAADLLEGEQVTIVDIDNGARLVTYAITGERGSGVIG
    INGAAAHLVHPGDLVILIAYATMDDARARTYQPRIVFVDAYNKPIDMGHDPAFVPENAGELLDPRLGVG
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A panCD mutant is attenuated in mice and induces significant protection from challenge with wild type M. tuberculosis (Sambandamurthy et al., 2002).
  • Related Vaccine(s): Mycobacterium tuberculosis panCD mutant vaccine , Mycobacterium tuberculosis RD1/panCD mutant vaccine
26. PE20
  • Gene Name : PE20
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0012371
  • NCBI Gene ID : 885537
  • NCBI Protein GI : 57116919
  • Locus Tag : Rv1806
  • Genbank Accession : BX842577
  • Protein Accession : YP_177843
  • Taxonomy ID : 83332
  • Gene Starting Position : 2048071
  • Gene Ending Position : 2048370
  • Gene Strand (Orientation) : ?
  • Protein Name : PE family protein
  • Protein pI : 4.19
  • Protein Weight : 8927.3
  • Protein Length : 99
  • Protein Note : Rv1806, (MTV049.28), len: 99 aa. Member of the Mycobacterium tuberculosis PE family of gly-, ala-rich proteins (see citation below), most similar to Rv1788|MTV049.10|AL022021 (99 aa), FASTA scores: opt: 334, E(): 4.7 e-15, (59.8% identity in 97 aa overlap). TBparse score is 0.932.
  • DNA Sequence : Show Sequence
    >gi|57116681:2048071-2048370 Mycobacterium tuberculosis H37Rv, complete genome
    CATGGCGTTTGTTCTTGTCTGTCCAGATGCGCTGGCCATCGCGGCCGGTCAGTTGCGCCATGTTGGATCG
    GTGATAGCCGCGCGGAATGCGGTCGCGGCACCGGCAACTGCCGAATTGGCCCCGGCGGCCGCTGACGAAG
    TATCAGCTTTGACTGCAACACAATTCAACTTCCATGCCGCCATGTACCAAGCGGTCGGCGCCCAGGCGAT
    CGCCATGAATGAGGCGTTCGTCGCGATGTTGGGCGCCAGCGCGGATTCTTACGCGGCTACCGAAGCCGCC
    AACATCATTGCTGTGAGCTA
  • Protein Sequence : Show Sequence
    >gi|57116919|ref|YP_177843.1| PE family protein [Mycobacterium tuberculosis H37Rv]
    MAFVLVCPDALAIAAGQLRHVGSVIAARNAVAAPATAELAPAAADEVSALTATQFNFHAAMYQAVGAQAI
    AMNEAFVAMLGASADSYAATEAANIIAVS
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : A selection of previously identified protective Mycobacterium tuberculosis DNA vaccines were re-formulated as proteins and administered with a Th1-inducing adjuvant to help stimulate the relevant immune responses necessary for protection. One candidate (Rv1806-1807) induced protection in the guinea pig aerosol infection model 30 days post-challenge on the basis of reducing the bacterial burden of M. tuberculosis in the lungs (Vipond et al., 2006).
27. PepA
  • Gene Name : PepA
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0010953
  • NCBI Gene ID : 886924
  • NCBI Protein GI : 15607267
  • Locus Tag : Rv0125
  • Genbank Accession : NC_000962.2
  • Protein Accession : NC_000962.2
  • Taxonomy ID : 1773
  • Gene Starting Position : 151148
  • Gene Ending Position : 152215
  • Gene Strand (Orientation) : +
  • Protein Name : PROBABLE SERINE PROTEASE PEPA (SERINE PROTEINASE) (MTB32A)
  • Protein pI : 4.91
  • Protein Weight : 34926
  • Protein Length : 355
  • Protein Note : Rv0125, (MTCI418B.07, MTB32A), len: 355 aa. Probable pepA (alternate gene name: mtb32a), serine protease (EC 3.4.21.-) (see Skeiky et al., 1999), highly similar to other proteases e.g. HHOB_ECOLI|P31137 protease hhob precursor (355 aa), FASTA scores: opt: 400, E(): 3.8e-14, (32.4% identity in 346 aa overlap). Also similar to Q50320 34 kDa PROTEIN PRECURSOR from Mycobacterium tuberculosis (361 aa), FASTA scores: opt: 1689, E(): 0, (70.7% identity in 362 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Has a putative signal sequence at the N-terminus. BELONGS TO THE SERINE PROTEASE FAMILY.; mtb32a
  • DNA Sequence : Show Sequence
    >GeneID|886924 [Mycobacterium tuberculosis H37Rv ] 151148..152215
    atgagcaattcgcgccgccgctcactcaggtggtcatggttgctgagcgtgctggctgccgtcgggctgg
    gcctggccacggcgccggcccaggcggccccgccggccttgtcgcaggaccggttcgccgacttccccgc
    gctgcccctcgacccgtccgcgatggtcgcccaagtggggccacaggtggtcaacatcaacaccaaactg
    ggctacaacaacgccgtgggcgccgggaccggcatcgtcatcgatcccaacggtgtcgtgctgaccaaca
    accacgtgatcgcgggcgccaccgacatcaatgcgttcagcgtcggctccggccaaacctacggcgtcga
    tgtggtcgggtatgaccgcacccaggatgtcgcggtgctgcagctgcgcggtgccggtggcctgccgtcg
    gcggcgatcggtggcggcgtcgcggttggtgagcccgtcgtcgcgatgggcaacagcggtgggcagggcg
    gaacgccccgtgcggtgcctggcagggtggtcgcgctcggccaaaccgtgcaggcgtcggattcgctgac
    cggtgccgaagagacattgaacgggttgatccagttcgatgccgcgatccagcccggtgattcgggcggg
    cccgtcgtcaacggcctaggacaggtggtcggtatgaacacggccgcgtccgataacttccagctgtccc
    agggtgggcagggattcgccattccgatcgggcaggcgatggcgatcgcgggccagatccgatcgggtgg
    ggggtcacccaccgttcatatcgggcctaccgccttcctcggcttgggtgttgtcgacaacaacggcaac
    ggcgcacgagtccaacgcgtggtcgggagcgctccggcggcaagtctcggcatctccaccggcgacgtga
    tcaccgcggtcgacggcgctccgatcaactcggccaccgcgatggcggacgcgcttaacgggcatcatcc
    cggtgacgtcatctcggtgacctggcaaaccaagtcgggcggcacgcgtacagggaacgtgacattggcc
    gagggacccccggcctga
  • Protein Sequence : Show Sequence
    >gi|15607267|ref|NP_214639.1| PROBABLE SERINE PROTEASE PEPA (SERINE PROTEINASE) (MTB32A) [Mycobacterium tuberculosis H37Rv ]
    MSNSRRRSLRWSWLLSVLAAVGLGLATAPAQAAPPALSQDRFADFPALPLDPSAMVAQVGPQVVNINTKL
    GYNNAVGAGTGIVIDPNGVVLTNNHVIAGATDINAFSVGSGQTYGVDVVGYDRTQDVAVLQLRGAGGLPS
    AAIGGGVAVGEPVVAMGNSGGQGGTPRAVPGRVVALGQTVQASDSLTGAEETLNGLIQFDAAIQPGDSGG
    PVVNGLGQVVGMNTAASDNFQLSQGGQGFAIPIGQAMAIAGQIRSGGGSPTVHIGPTAFLGLGVVDNNGN
    GARVQRVVGSAPAASLGISTGDVITAVDGAPINSATAMADALNGHHPGDVISVTWQTKSGGTRTGNVTLA
    EGPPA
  • Molecule Role : Protective antigen
  • Related Vaccine(s): Mtb72f fusion protein
28. PhoP
  • Gene Name : PhoP
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 888772
  • NCBI Protein GI : 15607897
  • Locus Tag : Rv0757
  • Genbank Accession : BX842574
  • Protein Accession : NP_215271
  • 3D structure: PDB ID : 3R0J
  • Taxonomy ID : 83332
  • Gene Starting Position : 851607
  • Gene Ending Position : 852350
  • Gene Strand (Orientation) : ?
  • Protein Name : two component system response transcriptional positive regulator PHOP
  • Protein pI : 6.31
  • Protein Weight : 24835.35
  • Protein Length : 247
  • Protein Note : Rv0757, (MTCY369.02), len: 247 aa. Possible phoP, two component system response phosphate regulon transcriptional regulator (see citations below), highly similar to various transcriptional regulators e.g. CAC32360.1|AL583945 putative two component system response regulator from Streptomyces coelicolor (271 aa); T45446 probable two-component response regulator from Mycobacterium leprae (253 aa); and similar to phoP proteins e.g. P13792|PHOP_BACSU alkaline phosphatase synthesis transcription regulatory protein from Bacillus subtilis (240 aa), FASTA scores: opt: 594, E(): 2.3e-33, (41.0% identity in 234 aa overlap); etc. Also highly similar to Rv3765c from Mycobacterium tuberculosis (234 aa), Rv1033c (257 aa), RV0903c|MTCY31.31c|Q10531 (236 aa), FASTA score: (45.4% identity in 229 aa overlap); MTCY10G2_16 and MTU88959_1.
  • DNA Sequence : Show Sequence
    >gi|57116681:851607-852350 Mycobacterium tuberculosis H37Rv, complete genome
    AATGCGGAAAGGGGTTGATCTCGTGACGGCGGGAACCCCAGGCGAAAACACCACACCGGAGGCTCGTGTC
    CTCGTGGTCGATGATGAGGCCAACATCGTTGAACTGCTGTCGGTGAGCCTCAAGTTCCAGGGCTTTGAAG
    TCTACACCGCGACCAACGGGGCACAGGCGCTGGATCGGGCCCGGGAAACCCGGCCGGACGCGGTGATCCT
    CGATGTGATGATGCCCGGGATGGACGGCTTTGGGGTGCTGCGCCGGCTGCGCGCCGACGGCATCGATGCC
    CCGGCGTTGTTCCTGACGGCCCGTGACTCGCTACAGGACAAGATCGCGGGTCTGACCCTGGGTGGTGACG
    ACTATGTGACAAAGCCCTTCAGTTTGGAGGAGGTCGTGGCCAGGCTGCGGGTCATCCTGCGACGCGCGGG
    CAAGGGCAACAAGGAACCACGTAATGTTCGACTGACGTTCGCCGATATCGAGCTCGACGAGGAGACCCAC
    GAAGTGTGGAAGGCGGGCCAACCGGTGTCGCTGTCGCCCACCGAATTCACCCTGCTGCGCTATTTCGTGA
    TCAACGCGGGCACCGTGCTGAGCAAGCCTAAGATTCTCGACCACGTTTGGCGCTACGACTTCGGTGGTGA
    TGTCAACGTCGTCGAGTCCTACGTGTCGTATCTGCGCCGCAAGATCGACACTGGGGAGAAGCGGCTGCTG
    CACACGCTGCGCGGGGTGGGCTACGTACTGCGGGAGCCTCGATG
  • Protein Sequence : Show Sequence
    >gi|15607897|ref|NP_215271.1| two component system response transcriptional positive regulator PHOP [Mycobacterium tuberculosis H37Rv]
    MRKGVDLVTAGTPGENTTPEARVLVVDDEANIVELLSVSLKFQGFEVYTATNGAQALDRARETRPDAVIL
    DVMMPGMDGFGVLRRLRADGIDAPALFLTARDSLQDKIAGLTLGGDDYVTKPFSLEEVVARLRVILRRAG
    KGNKEPRNVRLTFADIELDEETHEVWKAGQPVSLSPTEFTLLRYFVINAGTVLSKPKILDHVWRYDFGGD
    VNVVESYVSYLRRKIDTGEKRLLHTLRGVGYVLREPR
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Study evaluated immunogenicity and protection against Mycobacterium tuberculosis in rhesus monkeys with an attenuated M. tuberculosis with a disrupted phoP gene (SO2) as a single-dose vaccine. SO2 treatment showed reduced average lung bacterial counts (1.0 and 1.2 log respectively, compared with 0.4 log for BCG) and significant protective effect by reduction in C-reactive protein levels, body weight loss, and decrease of erythrocyte-associated hematologic parameters (MCV, MCH, Hb, Ht) as markers of inflammatory infection, all relative to non-vaccinated controls (Verreck et al., 2009).
  • Related Vaccine(s): M. tuberculosis phoP mutant SO2
29. PPE14
  • Gene Name : PPE14
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0012374
  • NCBI Gene ID : 885069
  • NCBI Protein GI : 57116795
  • Locus Tag : Rv0915c
  • Genbank Accession : BX842574
  • Protein Accession : YP_177765
  • Taxonomy ID : 83332
  • Gene Starting Position : 1020057
  • Gene Ending Position : 1021328
  • Gene Strand (Orientation) : -
  • Protein Name : PPE family protein
  • Protein pI : 4.31
  • Protein Weight : 39057.75
  • Protein Length : 423
  • Protein Note : Rv0915c, (MTCY21C12.09c), len: 423 aa. PPE14 (alternate gene name: MTB41). Member of the Mycobacterium tuberculosis PPE family (see citation below), highly similar to many e.g. Rv1807 from Mycobacterium tuberculosis (403 aa), FASTA scores: opt: 966, E(): 4.4e-30, (45.7% identity in 392 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2.; MTB41
  • DNA Sequence : Show Sequence
    >gi|57116681:1020057-1021328 Mycobacterium tuberculosis H37Rv, complete genome
    TTTACCGCGGGGGGTTTCCGGGCGGCGGCTGCTCTCTAATCACAACTACCGGGGGTTTGCGGCCGTCCTC
    TTGGCCGTCAGTGCTGGTGCCGCTACGGGTGCCGCCACCGCCCGTCGTGCCGCGTGCGGCCAGGCTCGCC
    AAAGCCATCCCGCTGAGCAGGCCTGCCGGCATCCCGTTTAGGGCCGTCGGGTCGGCGCCGGCGCTGGAGC
    TGAAGGTGGGTGTTGCCTGAACGGCGAGCTGGATCTCCGGGGCGGCCGTGGTCCAGCTGTGCGGCACCGA
    CAACGCTCCGACTAATGCTGCGTGGCCGACGCCCGCGGACACCGGCGCCGCGCCCCCGAAGGGGCCCCAG
    TGCGGCTCCGGCTCGTCGGTCGCCGAACTCAGTGGATGGCCCTGCGTCGGTCCCAGCCCGCCGGCGTTCC
    CGTATAGGCCGATGTGCCAGGGTCTGGCCGTGTTCGTGATCGCGAGCGCAATGCTGCCGGTCGCGATGGA
    TGCAATGTAGAGCGCGATCACGTCCAATTCCCCTATCGGGGTGGGGATCACTATCGGCTGAGCGGATCCG
    ACTTGCGGGTTGAGGGTCGACGCGATCCCCAACAGTCCCGATGTCAGCGGATCAGCGTTGGCGGCCAATG
    CGGACAGAATGTCGCTCAGGATCCCCGGGGGCAGCTGGGCCAGTGTCGCCTGTGCATCCGCAACGGCGCC
    CGCACCGGCGGCTTGGGTCGCCGCGGCTGCGGCCGCGGGCCCGGCCGGGCCGGTGCCTTGCACGGGTGGA
    GTGAACGGCGGCAACGCCGACGCGGCCGCAGATGCCCCCTCATAGCTGTACATCACGGCAGCGTCTTGGG
    CCCACATTTCGGCATACTCGGCCTGGGTAGCCGCGATCGCCGCACTGTTTTGCCCCAGAATGTTCGCCGC
    GACCAGCGACATCAACCGGCTGCGGTTGGCCGCGACGAGGGATGGTGGCACCGTCATCGCGAACGCCGTC
    CCAAACGCTTCCGCCGCTGCCCTCGCCTGTGTGGCCGTCTCCTTCGCCAGCGCCGCCGTGGCGGCCAGCC
    ACCCCACATACGGCGTTGCCGCGGCCGCCATCGCGGCCGCCGCCGGCCCCATCCACGGCTCAACGATCAG
    CGTCGACACCACCGATCCATACGAGACCGCGGCGGAAGTCAACTCCGCGGCCACACCGTCCCAGGCGGCC
    GCGGCGGCTAGCATCGACTCCGGCCCCGGACCGGAATACATTCGGCTTGAATTCACTTCCGGAGGTAAAA
    GCCCGAAATCCA
  • Protein Sequence : Show Sequence
    >gi|57116795|ref|YP_177765.1| PPE family protein [Mycobacterium tuberculosis H37Rv]
    MDFGLLPPEVNSSRMYSGPGPESMLAAAAAWDGVAAELTSAAVSYGSVVSTLIVEPWMGPAAAAMAAAAT
    PYVGWLAATAALAKETATQARAAAEAFGTAFAMTVPPSLVAANRSRLMSLVAANILGQNSAAIAATQAEY
    AEMWAQDAAVMYSYEGASAAASALPPFTPPVQGTGPAGPAAAAAATQAAGAGAVADAQATLAQLPPGILS
    DILSALAANADPLTSGLLGIASTLNPQVGSAQPIVIPTPIGELDVIALYIASIATGSIALAITNTARPWH
    IGLYGNAGGLGPTQGHPLSSATDEPEPHWGPFGGAAPVSAGVGHAALVGALSVPHSWTTAAPEIQLAVQA
    TPTFSSSAGADPTALNGMPAGLLSGMALASLAARGTTGGGGTRSGTSTDGQEDGRKPPVVVIREQPPPGN
    PPR
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : immunization with the soluble recombinant rMTB41 protein plus adjuvant, a regimen known to generate primarily CD4+ T cells, resulted in induction of protection comparable to BCG in two well-established animal models of tuberculosis (mice and guinea pigs) (Skeiky et al., 2005).
30. PPE18
  • Gene Name : PPE18
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0010954
  • NCBI Gene ID : 886073
  • NCBI Protein GI : 57116841
  • Locus Tag : Rv1196
  • Genbank Accession : NC_000962.2
  • Protein Accession : NC_000962.2
  • Taxonomy ID : 1773
  • Gene Starting Position : 1339349
  • Gene Ending Position : 1340524
  • Gene Strand (Orientation) : +
  • Protein Name : PPE FAMILY PROTEIN
  • Protein pI : 4.81
  • Protein Weight : 39158.1
  • Protein Length : 391
  • Protein Note : Rv1196, (MTCI364.08), len: 391 aa. PPE18 (alternate gene name: mtb39a). Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to others e.g. Y07P_MYCTU|Q11031 hypothetical 40.0 kDa protein cy02b10.25c (396 aa), FASTA scores: opt: 2124, E(): 0, (85.1% identity in 397 aa overlap). Note that expression of Rv1196 was demonstrated in lysates by immunodetection (see Dillon et al., 1999).; mtb39a
  • DNA Sequence : Show Sequence
    >GeneID|886073 [Mycobacterium tuberculosis H37Rv ] 1339349..1340524
    atggtggatttcggggcgttaccaccggagatcaactccgcgaggatgtacgccggcccgggttcggcct
    cgctggtggccgcggctcagatgtgggacagcgtggcgagtgacctgttttcggccgcgtcggcgtttca
    gtcggtggtctggggtctgacggtggggtcgtggataggttcgtcggcgggtctgatggtggcggcggcc
    tcgccgtatgtggcgtggatgagcgtcaccgcggggcaggccgagctgaccgccgcccaggtccgggttg
    ctgcggcggcctacgagacggcgtatgggctgacggtgcccccgccggtgatcgccgagaaccgtgctga
    actgatgattctgatagcgaccaacctcttggggcaaaacaccccggcgatcgcggtcaacgaggccgaa
    tacggcgagatgtgggcccaagacgccgccgcgatgtttggctacgccgcggcgacggcgacggcgacgg
    cgacgttgctgccgttcgaggaggcgccggagatgaccagcgcgggtgggctcctcgagcaggccgccgc
    ggtcgaggaggcctccgacaccgccgcggcgaaccagttgatgaacaatgtgccccaggcgctgcaacag
    ctggcccagcccacgcagggcaccacgccttcttccaagctgggtggcctgtggaagacggtctcgccgc
    atcggtcgccgatcagcaacatggtgtcgatggccaacaaccacatgtcgatgaccaactcgggtgtgtc
    gatgaccaacaccttgagctcgatgttgaagggctttgctccggcggcggccgcccaggccgtgcaaacc
    gcggcgcaaaacggggtccgggcgatgagctcgctgggcagctcgctgggttcttcgggtctgggcggtg
    gggtggccgccaacttgggtcgggcggcctcggtcggttcgttgtcggtgccgcaggcctgggccgcggc
    caaccaggcagtcaccccggcggcgcgggcgctgccgctgaccagcctgaccagcgccgcggaaagaggg
    cccgggcagatgctgggcgggctgccggtggggcagatgggcgccagggccggtggtgggctcagtggtg
    tgctgcgtgttccgccgcgaccctatgtgatgccgcattctccggcggccggctag
  • Protein Sequence : Show Sequence
    >gi|57116841|ref|YP_177795.1| PPE FAMILY PROTEIN [Mycobacterium tuberculosis H37Rv ]
    MVDFGALPPEINSARMYAGPGSASLVAAAQMWDSVASDLFSAASAFQSVVWGLTVGSWIGSSAGLMVAAA
    SPYVAWMSVTAGQAELTAAQVRVAAAAYETAYGLTVPPPVIAENRAELMILIATNLLGQNTPAIAVNEAE
    YGEMWAQDAAAMFGYAAATATATATLLPFEEAPEMTSAGGLLEQAAAVEEASDTAAANQLMNNVPQALQQ
    LAQPTQGTTPSSKLGGLWKTVSPHRSPISNMVSMANNHMSMTNSGVSMTNTLSSMLKGFAPAAAAQAVQT
    AAQNGVRAMSSLGSSLGSSGLGGGVAANLGRAASVGSLSVPQAWAAANQAVTPAARALPLTSLTSAAERG
    PGQMLGGLPVGQMGARAGGGLSGVLRVPPRPYVMPHSPAAG
  • Molecule Role : Protective antigen
  • Related Vaccine(s): Mtb72f fusion protein
31. PPE31
  • Gene Name : PPE31
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0012372
  • NCBI Gene ID : 885072
  • NCBI Protein GI : 57116920
  • Locus Tag : Rv1807
  • Genbank Accession : BX842577
  • Protein Accession : YP_177653
  • Taxonomy ID : 83332
  • Gene Starting Position : 2048397
  • Gene Ending Position : 2049596
  • Gene Strand (Orientation) : ?
  • Protein Name : PPE family protein
  • Protein pI : 4.25
  • Protein Weight : 36883.49
  • Protein Length : 399
  • Protein Note : Rv1807, (MTV049.29), len: 399 aa. Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv1789|MTV049.11|AL022021 (393 aa), FASTA scores: opt: 1169, E(): 0, (49.5% identity in 412 aa overlap). TBparse score is 0.919.
  • DNA Sequence : Show Sequence
    >gi|57116681:2048397-2049596 Mycobacterium tuberculosis H37Rv, complete genome
    ACTTGACTTCGCCACGCTACCGCCCGAAATCAACTCGGCGCGTATGTATTCCGGCGCGGGCTCGGCCCCG
    ATGCTGGCCGCAGCGTCAGCCTGGCACGGCTTGTCCGCAGAACTGCGCGCCAGCGCACTGTCATACAGCT
    CGGTGCTTTCGACGCTGACCGGTGAAGAATGGCACGGTCCGGCGTCGGCATCGATGACAGCCGCGGCCGC
    CCCCTACGTGGCCTGGATGAGCGTCACCGCCGTCCGGGCCGAGCAGGCCGGGGCACAGGCGGAGGCTGCC
    GCTGCAGCGTACGAAGCCGCGTTCGCAGCAACGGTGCCCCCGCCGGTCATCGAGGCCAACCGCGCCCAGC
    TCATGGCGCTGATCGCCACCAATGTGCTAGGCCAAAACGCCCCCGCGATCGCGGCCACCGAGGCCCAGTA
    CGCCGAAATGTGGTCCCAGGACGCGATGGCCATGTACGGCTACGCCGGCGCCTCGGCAGCCGCTACCCAG
    CTGACCCCGTTCACCGAGCCGGTGCAGACTACCAACGCGTCCGGCCTGGCGGCCCAGTCGGCTGCGATTG
    CCCACGCCACCGGCGCCTCGGCTGGTGCTCAGCAAACGACGCTGTCGCAGCTGATCGCCGCCATACCGTC
    TGTACTGCAAGGACTTTCGTCATCGACTGCAGCCACGTTCGCGTCGGGGCCGTCCGGATTGCTGGGCATT
    GTCGGGTCTGGATCTTCCTGGCTCGACAAACTCTGGGCGTTACTGGACCCCAACTCCAATTTCTGGAACA
    CGATAGCTTCGTCCGGACTGTTCTTGCCGAGTAACACGATTGCGCCCTTTTTGGGTCTACTCGGCGGCGT
    GGCAGCTGCGGATGCGGCCGGGGATGTGTTGGGAGAGGCCACCAGTGGCGGGCTCGGTGGCGCGCTGGTG
    GCGCCGCTTGGCTCAGCGGGCGGGCTAGGCGGCACTGTCGCGGCCGGCCTGGGCAACGCGGCCACCGTCG
    GAACCTTGTCGGTGCCGCCGAGCTGGACGGCGGCCGCACCACTAGCCAGCCCCTTGGGCTCCGCGTTGGG
    AGGCACACCGATGGTGGCACCGCCCCCAGCAGTGGCGGCCGGCATGCCCGGAATGCCTTTCGGCACCATG
    GGCGGTCAAGGCTTCGGGCGTGCCGTGCCCCAGTATGGCTTCCGCCCCAACTTCGTCGCACGACCGCCCG
    CCGCCGGGTG
  • Protein Sequence : Show Sequence
    >gi|57116920|ref|YP_177653.1| PPE family protein [Mycobacterium tuberculosis H37Rv]
    LDFATLPPEINSARMYSGAGSAPMLAAASAWHGLSAELRASALSYSSVLSTLTGEEWHGPASASMTAAAA
    PYVAWMSVTAVRAEQAGAQAEAAAAAYEAAFAATVPPPVIEANRAQLMALIATNVLGQNAPAIAATEAQY
    AEMWSQDAMAMYGYAGASAAATQLTPFTEPVQTTNASGLAAQSAAIAHATGASAGAQQTTLSQLIAAIPS
    VLQGLSSSTAATFASGPSGLLGIVGSGSSWLDKLWALLDPNSNFWNTIASSGLFLPSNTIAPFLGLLGGV
    AAADAAGDVLGEATSGGLGGALVAPLGSAGGLGGTVAAGLGNAATVGTLSVPPSWTAAAPLASPLGSALG
    GTPMVAPPPAVAAGMPGMPFGTMGGQGFGRAVPQYGFRPNFVARPPAAG
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : A selection of previously identified protective Mycobacterium tuberculosis DNA vaccines were re-formulated as proteins and administered with a Th1-inducing adjuvant to help stimulate the relevant immune responses necessary for protection. One candidate (Rv1806-1807) induced protection in the guinea pig aerosol infection model 30 days post-challenge on the basis of reducing the bacterial burden of M. tuberculosis in the lungs (Vipond et al., 2006).
32. proC
  • Gene Name : proC
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 887256
  • NCBI Protein GI : 15607641
  • Locus Tag : Rv0500
  • Genbank Accession : BX842573
  • Protein Accession : NP_215014
  • Taxonomy ID : 83332
  • Gene Starting Position : 590082
  • Gene Ending Position : 590969
  • Gene Strand (Orientation) : ?
  • Protein Name : pyrroline-5-carboxylate reductase
  • Protein pI : 4.42
  • Protein Weight : 26600.99
  • Protein Length : 295
  • Protein Note : catalyzes the formation of L-proline from pyrroline-5-carboxylate
  • DNA Sequence : Show Sequence
    >gi|57116681:590082-590969 Mycobacterium tuberculosis H37Rv, complete genome
    GATGCTTTTCGGCATGGCAAGGATCGCGATTATCGGCGGCGGCAGCATCGGTGAGGCATTGCTGTCGGGT
    CTGCTGCGGGCGGGCCGGCAGGTCAAAGACCTGGTAGTGGCCGAGCGGATGCCCGATCGCGCCAACTACC
    TGGCGCAGACCTATTCGGTGTTGGTGACGTCGGCGGCCGACGCGGTGGAGAACGCGACGTTCGTCGTCGT
    CGCGGTCAAACCAGCCGACGTCGAGCCGGTGATCGCGGATCTGGCGAACGCGACTGCGGCGGCCGAAAAC
    GACAGTGCTGAGCAGGTGTTCGTCACCGTGGTAGCGGGCATCACGATCGCGTATTTCGAATCCAAGCTAC
    CGGCTGGGACGCCAGTGGTGCGTGCGATGCCGAACGCGGCGGCATTGGTGGGAGCGGGGGTTACAGCGCT
    GGCCAAAGGCCGCTTTGTCACCCCGCAACAGCTTGAGGAGGTCTCGGCCTTGTTCGACGCGGTCGGCGGC
    GTGCTGACCGTTCCGGAATCGCAGTTGGACGCGGTGACCGCGGTGTCCGGCTCGGGTCCGGCCTATTTCT
    TTCTGCTGGTCGAGGCCCTGGTGGATGCCGGAGTCGGGGTGGGCTTGAGCCGTCAGGTGGCCACCGATCT
    CGCCGCGCAGACAATGGCTGGCTCAGCGGCGATGCTGCTGGAGCGGATGGAGCAAGACCAGGGTGGCGCC
    AATGGCGAGCTGATGGGGCTGCGCGTGGACCTTACCGCATCACGGCTGCGCGCCGCGGTTACCTCGCCGG
    GCGGTACGACCGCCGCTGCGCTGCGGGAACTCGAACGCGGCGGGTTTCGGATGGCTGTCGACGCGGCGGT
    TCAAGCCGCCAAAAGCCGCTCTGAGCAGCTCAGAATTACACCGGAATG
  • Protein Sequence : Show Sequence
    >gi|15607641|ref|NP_215014.1| pyrroline-5-carboxylate reductase [Mycobacterium tuberculosis H37Rv]
    MLFGMARIAIIGGGSIGEALLSGLLRAGRQVKDLVVAERMPDRANYLAQTYSVLVTSAADAVENATFVVV
    AVKPADVEPVIADLANATAAAENDSAEQVFVTVVAGITIAYFESKLPAGTPVVRAMPNAAALVGAGVTAL
    AKGRFVTPQQLEEVSALFDAVGGVLTVPESQLDAVTAVSGSGPAYFFLLVEALVDAGVGVGLSRQVATDL
    AAQTMAGSAAMLLERMEQDQGGANGELMGLRVDLTASRLRAAVTSPGGTTAAALRELERGGFRMAVDAAV
    QAAKSRSEQLRITPE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A proC mutant is highly attenuated in mice and induces significant protection from challenge with wild type M. tuberculosis that is equal to the protection induced by the current vaccine BCG (Smith et al., 2001).
  • Related Vaccine(s): Mycobacterium tuberculosis proC mutant vaccine
33. PstS1
  • Gene Name : PstS1
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0012407
  • NCBI Gene ID : 885724
  • NCBI Protein GI : 57116801
  • Locus Tag : Rv0934
  • Genbank Accession : BX842575
  • Protein Accession : YP_177770
  • 3D structure: PDB ID : 1PC3
  • Taxonomy ID : 83332
  • Gene Starting Position : 1042114
  • Gene Ending Position : 1043238
  • Gene Strand (Orientation) : ?
  • Protein Name : periplasmic phosphate-binding lipoprotein PSTS1 (PBP-1) (PSTS1)
  • Protein pI : 5.02
  • Protein Weight : 36356.95
  • Protein Length : 374
  • Protein Note : Rv0934, (MTCY08D9.05c), len: 374 aa. pstS1 (previously known as phoS1 or phoS), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to Rv0932c|MTCY08D9.07|pstS2 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN from Mycobacterium tuberculosis (370 aa), FASTA scores: opt: 460, E(): 5.9e-19, (31.2% identity in 375 aa overlap); and Rv0928|MTCY21C12.22|pstS3 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 435, E():1.1e-17, (30.0% identity in 380 aa overlap) (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0928c). Also highly similar to MTCY08D9.05c|P15712|PAB_MYCTU PROTEIN ANTIGEN B PRECURSOR from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 2459, E(): 0, (100% identity in 374 aa overlap). Contains a prokaryotic membrane lipoprotein lipid attachment site (PS00013) at the N-terminus so the 23 aa leader peptide sequence is probably removed. BELONGS TO FAMILY OF PHOSPHATE RECEPTORS FOR BACTERIAL ABC-TYPE LIPOPROTEIN TRANSPORTERS.; phoS1; phoS
  • DNA Sequence : Show Sequence
    >gi|57116681:1042114-1043238 Mycobacterium tuberculosis H37Rv, complete genome
    CGTGAAAATTCGTTTGCATACGCTGTTGGCCGTGTTGACCGCTGCGCCGCTGCTGCTAGCAGCGGCGGGC
    TGTGGCTCGAAACCACCGAGCGGTTCGCCTGAAACGGGCGCCGGCGCCGGTACTGTCGCGACTACCCCCG
    CGTCGTCGCCGGTGACGTTGGCGGAGACCGGTAGCACGCTGCTCTACCCGCTGTTCAACCTGTGGGGTCC
    GGCCTTTCACGAGAGGTATCCGAACGTCACGATCACCGCTCAGGGCACCGGTTCTGGTGCCGGGATCGCG
    CAGGCCGCCGCCGGGACGGTCAACATTGGGGCCTCCGACGCCTATCTGTCGGAAGGTGATATGGCCGCGC
    ACAAGGGGCTGATGAACATCGCGCTAGCCATCTCCGCTCAGCAGGTCAACTACAACCTGCCCGGAGTGAG
    CGAGCACCTCAAGCTGAACGGAAAAGTCCTGGCGGCCATGTACCAGGGCACCATCAAAACCTGGGACGAC
    CCGCAGATCGCTGCGCTCAACCCCGGCGTGAACCTGCCCGGCACCGCGGTAGTTCCGCTGCACCGCTCCG
    ACGGGTCCGGTGACACCTTCTTGTTCACCCAGTACCTGTCCAAGCAAGATCCCGAGGGCTGGGGCAAGTC
    GCCCGGCTTCGGCACCACCGTCGACTTCCCGGCGGTGCCGGGTGCGCTGGGTGAGAACGGCAACGGCGGC
    ATGGTGACCGGTTGCGCCGAGACACCGGGCTGCGTGGCCTATATCGGCATCAGCTTCCTCGACCAGGCCA
    GTCAACGGGGACTCGGCGAGGCCCAACTAGGCAATAGCTCTGGCAATTTCTTGTTGCCCGACGCGCAAAG
    CATTCAGGCCGCGGCGGCTGGCTTCGCATCGAAAACCCCGGCGAACCAGGCGATTTCGATGATCGACGGG
    CCCGCCCCGGACGGCTACCCGATCATCAACTACGAGTACGCCATCGTCAACAACCGGCAAAAGGACGCCG
    CCACCGCGCAGACCTTGCAGGCATTTCTGCACTGGGCGATCACCGACGGCAACAAGGCCTCGTTCCTCGA
    CCAGGTTCATTTCCAGCCGCTGCCGCCCGCGGTGGTGAAGTTGTCTGACGCGTTGATCGCGACGATTTCC
    AGCTA
  • Protein Sequence : Show Sequence
    >gi|57116801|ref|YP_177770.1| periplasmic phosphate-binding lipoprotein PSTS1 (PBP-1) (PSTS1) [Mycobacterium tuberculosis H37Rv]
    MKIRLHTLLAVLTAAPLLLAAAGCGSKPPSGSPETGAGAGTVATTPASSPVTLAETGSTLLYPLFNLWGP
    AFHERYPNVTITAQGTGSGAGIAQAAAGTVNIGASDAYLSEGDMAAHKGLMNIALAISAQQVNYNLPGVS
    EHLKLNGKVLAAMYQGTIKTWDDPQIAALNPGVNLPGTAVVPLHRSDGSGDTFLFTQYLSKQDPEGWGKS
    PGFGTTVDFPAVPGALGENGNGGMVTGCAETPGCVAYIGISFLDQASQRGLGEAQLGNSSGNFLLPDAQS
    IQAAAAGFASKTPANQAISMIDGPAPDGYPIINYEYAIVNNRQKDAATAQTLQAFLHWAITDGNKASFLD
    QVHFQPLPPAVVKLSDALIATISS
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Vaccination with the recombinant PstS-1 antigen and cholera toxin significantly protected against the challenge given 10 days later, but only marginally after 12 weeks (Falero-Diaz et al., 2000).
34. PstS3
  • Gene Name : PstS3
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0012370
  • NCBI Gene ID : 885366
  • NCBI Protein GI : 57116798
  • Locus Tag : Rv0928
  • Genbank Accession : BX842575
  • Protein Accession : YP_177768
  • Taxonomy ID : 83332
  • Gene Starting Position : 1034902
  • Gene Ending Position : 1036014
  • Gene Strand (Orientation) : ?
  • Protein Name : periplasmic phosphate-binding lipoprotein PSTS3 (PBP-3) (PSTS3) (PHOS1)
  • Protein pI : 5.85
  • Protein Weight : 35472.01
  • Protein Length : 370
  • Protein Note : Rv0928, (MTCY21C12.22), len: 370 aa. pstS3 (previously known as phoS2), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to others from Mycobacterium leprae e.g. Q50099|PSTS3|PHOS1 phosphate-binding protein 3 precursor (328 aa), FASTA scores: opt: 1772, E(): 0, (79.6% identity in 328 aa overlap); and highly similar to others e.g. AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa). Also highly similar to Rv0932c|MTCY08D9.07|pstS2 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN (370 aa); and Rv0934|pstS1 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN (374 aa) from Mycobacterium tuberculosis (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0934c). Contains lipoprotein signature (PS00013) at N-terminus. BELONGS TO FAMILY OF PHOSPHATE RECEPTORS FOR BACTERIAL ABC-TYPE LIPOPROTEIN TRANSPORTERS.; phoS2
  • DNA Sequence : Show Sequence
    >gi|57116681:1034902-1036014 Mycobacterium tuberculosis H37Rv, complete genome
    ATTGAAACTCAACCGATTTGGTGCCGCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCCGCGTGT
    GGTAACGACGACAATGTGACCGGGGGAGGTGCAACCACTGGCCAGGCGTCGGCGAAGGTCGATTGCGGGG
    GGAAGAAGACACTCAAAGCCAGTGGGTCGACGGCGCAGGCCAACGCGATGACCCGCTTTGTCAACGTGTT
    CGAGCAGGCCTGCCCCGGCCAAACCCTGAACTACACGGCCAATGGTTCGGGCGCTGGAATCAGCGAATTT
    AATGGCAACCAAACCGATTTCGGTGGCTCAGATGTACCCCTGAGCAAGGACGAGGCCGCAGCGGCGCAGC
    GGCGTTGCGGCTCGCCGGCGTGGAATCTGCCGGTGGTGTTCGGCCCGATCGCGGTTACCTACAACCTCAA
    CAGCGTTTCCTCGCTAAATTTGGACGGCCCCACGTTGGCGAAGATCTTCAACGGCTCCATTACGCAGTGG
    AACAATCCCGCGATCCAGGCGCTGAACCGCGACTTCACGCTGCCAGGTGAGCGGATTCACGTGGTGTTCC
    GCAGCGATGAGTCGGGGACCACGGACAACTTCCAGAGGTACCTGCAGGCCGCGTCCAACGGTGCGTGGGG
    TAAGGGCGCTGGAAAGTCGTTCCAAGGCGGCGTCGGTGAGGGCGCGCGGGGTAACGATGGCACGTCAGCG
    GCCGCGAAGAACACCCCGGGGTCGATCACCTACAACGAGTGGTCGTTCGCCCAGGCGCAGCACCTGACCA
    TGGCCAACATCGTCACTTCGGCTGGTGGGGACCCGGTGGCGATTACTATCGACTCGGTCGGCCAGACGAT
    CGCCGGGGCCACCATCTCCGGGGTGGGCAACGACCTGGTGCTCGACACGGACTCGTTCTACCGGCCGAAG
    CGTCCCGGCTCCTATCCGATCGTGTTAGCGACATACGAAATCGTTTGCTCGAAGTATCCCGACTCGCAGG
    TTGGCACGGCTGTGAAGGCGTTCCTGCAGAGCACTATCGGCGCCGGTCAAAGCGGCCTGGGGGACAACGG
    ATACATCCCAATTCCGGACGAGTTCAAATCGAGGCTGTCGACTGCGGTCAACGCGATCGCCTG
  • Protein Sequence : Show Sequence
    >gi|57116798|ref|YP_177768.1| periplasmic phosphate-binding lipoprotein PSTS3 (PBP-3) (PSTS3) (PHOS1) [Mycobacterium tuberculosis H37Rv]
    MKLNRFGAAVGVLAAGALVLSACGNDDNVTGGGATTGQASAKVDCGGKKTLKASGSTAQANAMTRFVNVF
    EQACPGQTLNYTANGSGAGISEFNGNQTDFGGSDVPLSKDEAAAAQRRCGSPAWNLPVVFGPIAVTYNLN
    SVSSLNLDGPTLAKIFNGSITQWNNPAIQALNRDFTLPGERIHVVFRSDESGTTDNFQRYLQAASNGAWG
    KGAGKSFQGGVGEGARGNDGTSAAAKNTPGSITYNEWSFAQAQHLTMANIVTSAGGDPVAITIDSVGQTI
    AGATISGVGNDLVLDTDSFYRPKRPGSYPIVLATYEIVCSKYPDSQVGTAVKAFLQSTIGAGQSGLGDNG
    YIPIPDEFKSRLSTAVNAIA
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice vaccinated with PstS-3 DNA demonstrated significant and sustained reduction in bacterial CFU numbers in spleen and lungs for 3 mo after M. tuberculosis challenge, as compared with CFU counts in mice vaccinated with control DNA (Tanghe et al., 1999).
35. RD1
  • Gene Name : RD1
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 885777
  • NCBI Protein GI : 15610750
  • Locus Tag : Rv3614c
  • Genbank Accession : BX842583
  • Protein Accession : NP_218131
  • Taxonomy ID : 83332
  • Gene Starting Position : 4054141
  • Gene Ending Position : 4054695
  • Gene Strand (Orientation) : -
  • Protein Name : hypothetical protein
  • Protein pI : 3.73
  • Protein Weight : 18643.7
  • Protein Length : 184
  • Protein Note : Rv3614c, (MTCY07H7B.08), len: 184 aa. Conserved hypothetical protein, equivalent to Q49730|ML0407|B1620_C3_264|MLCL383.03 HYPOTHETICAL 24.2 KDA PROTEIN from Mycobacterium leprae (216 aa) FASTA scores: opt: 899, E(): 1.7e-51, (71.3% identity in 188 aa overlap); and similar to two hypothetical proteins from Mycobacterium leprae: Q9CDD6|ML0056 (169 aa), FASTA scores: opt: 285, E(): 1.2e-11, (38.35% identity in 172 aa overlap); and O33090|MLCB628.19c (338 aa), FASTA scores: opt: 289, E(): 1.2e-11, (38.95% identity in 172 aa overlap). Also highly similar to O69732|Rv3867|MTV027.02 HYPOTHETICAL 19.9 KDA PROTEIN from Mycobacterium tuberculosis (183 aa), FASTA scores: opt: 563, E(): 1e-29, (54.9% identity in 173 aa overlap).
  • DNA Sequence : Show Sequence
    >gi|57116681:4054141-4054695 Mycobacterium tuberculosis H37Rv, complete genome
    CTCACCATGGATCGCTCTCGTCGTCTGGTGCTGGACAATCGTCGCTGTAGCGCGTCGCGAACACCTCAGC
    TTCTGCTGCCGCGGCTTCTTCCGGCGATGGTAACCCCCAGGTTTCGCCCACGGTCTTACGTAGCAGTGCG
    ACGCGGTGTTCATCTGCATCGACCTGTTGACTCATCCTGTCAAGGATGAAGGCGTACTGGGCCGACTGCG
    CCTTCTGCCGCGCCAGGTCGGCAATCACCAGGATCTCAGAAGCGAGCTGCGACTCACTCATCCAGGCCAC
    CCTGGCCGACAGCTCGACATGGTCAATCCGGCCGTCCATCAGCGTCGATACCGACACCGTGCGTGGGGGA
    TTCGTCACGGTAAAAAGCGCGATCTCTTGTTCGGTGTCCGTCTCCGCCTGACCGTGGGCATTGTCCAGGT
    CGGGTCCGGTGTCCGGGGTCGCCGCCGACCCGACGCCAATAATCGGATCCGCAGTCCAGCCCTCCGCGCC
    GTCGGCACCCCAGAGATCCACGGCGTCGAAATCGTTGCTGTCAAAGTCATTTCCGGGCAAGTCCA
  • Protein Sequence : Show Sequence
    >gi|15610750|ref|NP_218131.1| hypothetical protein Rv3614c [Mycobacterium tuberculosis H37Rv]
    MDLPGNDFDSNDFDAVDLWGADGAEGWTADPIIGVGSAATPDTGPDLDNAHGQAETDTEQEIALFTVTNP
    PRTVSVSTLMDGRIDHVELSARVAWMSESQLASEILVIADLARQKAQSAQYAFILDRMSQQVDADEHRVA
    LLRKTVGETWGLPSPEEAAAAEAEVFATRYSDDCPAPDDESDPW
  • Molecule Role : Virmugen
  • Molecule Role Annotation : RD1 and panCD mutants in Mycobacterium tuberculosis were attenuated and provided protection in CD4-deficient mice against an aerosol challenge with virulent M. tuberculosis (Sambandamurthy et al., 2006).
  • Related Vaccine(s): Mycobacterium tuberculosis RD1/panCD mutant vaccine
36. Rv1806
  • Gene Name : Rv1806
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 885537
  • NCBI Protein GI : 57116919
  • Locus Tag : Rv1806
  • Genbank Accession : CP003248
  • Protein Accession : YP_177843
  • Taxonomy ID : 83332
  • Gene Starting Position : 2048071
  • Gene Ending Position : 2048370
  • Gene Strand (Orientation) : ?
  • Protein Name : PE family protein PE20
  • Protein pI : 4.19
  • Protein Weight : 8927.3
  • Protein Length : 99
  • Protein Note : Rv1806, (MTV049.28), len: 99 aa. PE20, Member of the Mycobacterium tuberculosis PE family of gly-, ala-rich proteins (see citation below), most similar to Rv1788|MTV049.10|AL022021 (99 aa), FASTA scores: opt: 334,E(): 4.7 e-15, (59.8% identity in 97 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).
  • DNA Sequence : Show Sequence
    >gi|448814763:2048071-2048370 Mycobacterium tuberculosis H37Rv complete genome
    CATGGCGTTTGTTCTTGTCTGTCCAGATGCGCTGGCCATCGCGGCCGGTCAGTTGCGCCATGTTGGATCG
    GTGATAGCCGCGCGGAATGCGGTCGCGGCACCGGCAACTGCCGAATTGGCCCCGGCGGCCGCTGACGAAG
    TATCAGCTTTGACTGCAACACAATTCAACTTCCATGCCGCCATGTACCAAGCGGTCGGCGCCCAGGCGAT
    CGCCATGAATGAGGCGTTCGTCGCGATGTTGGGCGCCAGCGCGGATTCTTACGCGGCTACCGAAGCCGCC
    AACATCATTGCTGTGAGCTA
  • Protein Sequence : Show Sequence
    >gi|57116919|ref|YP_177843.1| PE family protein PE20 [Mycobacterium tuberculosis H37Rv]
    MAFVLVCPDALAIAAGQLRHVGSVIAARNAVAAPATAELAPAAADEVSALTATQFNFHAAMYQAVGAQAI
    AMNEAFVAMLGASADSYAATEAANIIAVS
  • Molecule Role : Protective antigen
  • Related Vaccine(s): DNA vaccine Rv1806-1807
37. Rv1807
  • Gene Name : Rv1807
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 885072
  • NCBI Protein GI : 57116920
  • Locus Tag : Rv1807
  • Genbank Accession : AL123456
  • Protein Accession : YP_177653
  • Taxonomy ID : 83332
  • Gene Starting Position : 2048397
  • Gene Ending Position : 2049596
  • Gene Strand (Orientation) : ?
  • Protein Name : PPE family protein PPE31
  • Protein pI : 4.25
  • Protein Weight : 36883.49
  • Protein Length : 399
  • Protein Note : Rv1807, (MTV049.29), len: 399 aa. PPE31, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv1789|MTV049.11|AL022021 (393 aa), FASTA scores: opt: 1169, E(): 0, (49.5% identity in 412 aa overlap).
  • DNA Sequence : Show Sequence
    >gi|448814763:2048397-2049596 Mycobacterium tuberculosis H37Rv complete genome
    ACTTGACTTCGCCACGCTACCGCCCGAAATCAACTCGGCGCGTATGTATTCCGGCGCGGGCTCGGCCCCG
    ATGCTGGCCGCAGCGTCAGCCTGGCACGGCTTGTCCGCAGAACTGCGCGCCAGCGCACTGTCATACAGCT
    CGGTGCTTTCGACGCTGACCGGTGAAGAATGGCACGGTCCGGCGTCGGCATCGATGACAGCCGCGGCCGC
    CCCCTACGTGGCCTGGATGAGCGTCACCGCCGTCCGGGCCGAGCAGGCCGGGGCACAGGCGGAGGCTGCC
    GCTGCAGCGTACGAAGCCGCGTTCGCAGCAACGGTGCCCCCGCCGGTCATCGAGGCCAACCGCGCCCAGC
    TCATGGCGCTGATCGCCACCAATGTGCTAGGCCAAAACGCCCCCGCGATCGCGGCCACCGAGGCCCAGTA
    CGCCGAAATGTGGTCCCAGGACGCGATGGCCATGTACGGCTACGCCGGCGCCTCGGCAGCCGCTACCCAG
    CTGACCCCGTTCACCGAGCCGGTGCAGACTACCAACGCGTCCGGCCTGGCGGCCCAGTCGGCTGCGATTG
    CCCACGCCACCGGCGCCTCGGCTGGTGCTCAGCAAACGACGCTGTCGCAGCTGATCGCCGCCATACCGTC
    TGTACTGCAAGGACTTTCGTCATCGACTGCAGCCACGTTCGCGTCGGGGCCGTCCGGATTGCTGGGCATT
    GTCGGGTCTGGATCTTCCTGGCTCGACAAACTCTGGGCGTTACTGGACCCCAACTCCAATTTCTGGAACA
    CGATAGCTTCGTCCGGACTGTTCTTGCCGAGTAACACGATTGCGCCCTTTTTGGGTCTACTCGGCGGCGT
    GGCAGCTGCGGATGCGGCCGGGGATGTGTTGGGAGAGGCCACCAGTGGCGGGCTCGGTGGCGCGCTGGTG
    GCGCCGCTTGGCTCAGCGGGCGGGCTAGGCGGCACTGTCGCGGCCGGCCTGGGCAACGCGGCCACCGTCG
    GAACCTTGTCGGTGCCGCCGAGCTGGACGGCGGCCGCACCACTAGCCAGCCCCTTGGGCTCCGCGTTGGG
    AGGCACACCGATGGTGGCACCGCCCCCAGCAGTGGCGGCCGGCATGCCCGGAATGCCTTTCGGCACCATG
    GGCGGTCAAGGCTTCGGGCGTGCCGTGCCCCAGTATGGCTTCCGCCCCAACTTCGTCGCACGACCGCCCG
    CCGCCGGGTG
  • Protein Sequence : Show Sequence
    >gi|57116920|ref|YP_177653.1| PPE family protein PPE31, partial [Mycobacterium tuberculosis H37Rv]
    LDFATLPPEINSARMYSGAGSAPMLAAASAWHGLSAELRASALSYSSVLSTLTGEEWHGPASASMTAAAA
    PYVAWMSVTAVRAEQAGAQAEAAAAAYEAAFAATVPPPVIEANRAQLMALIATNVLGQNAPAIAATEAQY
    AEMWSQDAMAMYGYAGASAAATQLTPFTEPVQTTNASGLAAQSAAIAHATGASAGAQQTTLSQLIAAIPS
    VLQGLSSSTAATFASGPSGLLGIVGSGSSWLDKLWALLDPNSNFWNTIASSGLFLPSNTIAPFLGLLGGV
    AAADAAGDVLGEATSGGLGGALVAPLGSAGGLGGTVAAGLGNAATVGTLSVPPSWTAAAPLASPLGSALG
    GTPMVAPPPAVAAGMPGMPFGTMGGQGFGRAVPQYGFRPNFVARPPAAG
  • Molecule Role : Protective antigen
  • Related Vaccine(s): DNA vaccine Rv1806-1807
38. Rv2660c
  • Gene Name : Rv2660c
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 887222
  • NCBI Protein GI : 15609797
  • Locus Tag : Rv2660c
  • Genbank Accession : BX842580
  • Protein Accession : NP_217176
  • Taxonomy ID : 83332
  • Gene Starting Position : 2980962
  • Gene Ending Position : 2981189
  • Gene Strand (Orientation) : -
  • Protein Name : hypothetical protein
  • Protein pI : 4.63
  • Protein Weight : 6788.85
  • Protein Length : 75
  • Protein Note : Rv2660c, (MTCY441.29c), len: 75 aa (questionable orf). Hypothetical unknown protein.
  • DNA Sequence : Show Sequence
    >gi|57116681:2980962-2981189 Mycobacterium tuberculosis H37Rv, complete genome
    ACTAGTGAAACTGGTTCAATCCCAGTATCGCGCACCACGATTGACCTGCGGTTTCATCCACAAAATCTGG
    GCTGCGTGAACTAAATGTGAACTGACTCGGTGCAACCACCGAAAGGTTCCTCTGTTCCGTGCCCACGCCG
    ACACCGACGGTGACCCCACCAGATGCGCCTGCCGCCCGCTGGCTAGCCTGGCCTGTTGCTGCAAGCGCCT
    GGTCGACGCCCGCTATCA
  • Protein Sequence : Show Sequence
    >gi|15609797|ref|NP_217176.1| hypothetical protein Rv2660c [Mycobacterium tuberculosis H37Rv]
    MIAGVDQALAATGQASQRAAGASGGVTVGVGVGTEQRNLSVVAPSQFTFSSRSPDFVDETAGQSWCAILG
    LNQFH
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : When combined with AG85B and ESAT-6, Rv2660c provided mice protection against challenge (Aagaard et al., 2011).
39. SecA2
  • Gene Name : SecA2
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • VO ID : VO_0010950
  • NCBI Gene ID : 885594
  • NCBI Protein GI : 15608958
  • Locus Tag : Rv1821
  • Genbank Accession : BX842577
  • Protein Accession : NP_216337
  • 3D structure: PDB ID : 1NL3
  • Other Database IDs : EMBL: AE000516
    EMBL: BX842577
    GenomeReviews: AE000516_GR
    GenomeReviews: AL123456_GR
    HSSP: O05885
    InterPro: IPR000185
    InterPro: IPR001650
    InterPro: IPR011115
    InterPro: IPR011116
    InterPro: IPR011130
    InterPro: IPR014018
    InterPro: IPR014021
    KEGG: mtc:MT1869
    KEGG: mtu:Rv1821
    Pfam: PF01043
    Pfam: PF07516
    Pfam: PF07517
    PIR: F70720
    PRINTS: PR00906
    PROSITE: PS01312
    PROSITE: PS51196
    TIGR: MT1869
    TubercuList: Rv1821
    UniProt: P66785
  • Taxonomy ID : 83332
  • Gene Starting Position : 2066456
  • Gene Ending Position : 2068882
  • Gene Strand (Orientation) : +
  • Protein Name : preprotein translocase subunit SecA
  • Protein pI : 5.11
  • Protein Weight : 82502.57
  • Protein Length : 808
  • Protein Note : SecA2; functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond to SecA2; which is non-essential and seems to play a role in secretion of a subset of proteins
  • Protein Annotation : FUNCTION: Involved in protein export. Interacts with the secY/secE subunits. SecA has a central role in coupling the hydrolysis of ATP to the transfer of pre-secretory proteins across the membrane (By similarity) (UniProt: P66785)

    SUBUNIT: Part of the prokaryotic protein translocation apparatus which comprise secA, secB, secD, secE, secF, secG and secY (By similarity) (UniProt: P66785)

    SUBCELLULAR LOCATION: Cell membrane; peripheral membrane protein; cytoplasmic side (By similarity) (UniProt: P66785)

    SIMILARITY: Belongs to the secA family (UniProt: P66785)
  • DNA Sequence : Show Sequence
    >gi|57116681:2066456-2068882 Mycobacterium tuberculosis H37Rv, complete genome
    CGTGAACGTGCACGGTTGTCCACGAATTGCGGCCTGTCGGTGTACAGACACGCACCCTCGCGGCCGGCCG
    GCATTCGCGTACCGTTGGTTTGTGCCCAAGACCACCCGCGCTCAACCCGGCCGGCTGAGCAGCCGATTCT
    GGCGATTGCTCGGCGCCAGCACCGAAAAGAACCGGAGCCGCTCCCTGGCGGATGTAACCGCTTCGGCAGA
    ATACGACAAGGAAGCTGCCGATCTGTCCGACGAGAAGCTGCGTAAGGCGGCAGGCCTGCTCAACCTCGAC
    GACCTCGCGGAGTCCGCCGATATCCCGCAGTTTCTCGCGATTGCCCGGGAAGCCGCCGAGCGGAGGACCG
    GGCTGCGACCATTTGATGTGCAGTTGCTTGGCGCGTTGCGCATGCTCGCCGGAGACGTGATCGAGATGGC
    CACCGGTGAGGGCAAAACCCTTGCCGGGGCGATCGCGGCCGCCGGTTATGCGCTGGCCGGCCGGCACGTG
    CACGTCGTGACGATTAACGATTACCTGGCCCGCCGCGATGCGGAGTGGATGGGCCCGCTGCTGGACGCGA
    TGGGCCTGACGGTCGGCTGGATCACCGCGGACTCGACCCCTGACGAGCGCCGGACCGCATATGACCGTGA
    TGTCACCTATGCCTCGGTCAACGAGATTGGCTTCGATGTACTGCGCGATCAGTTGGTGACTGATGTCAAT
    GACCTGGTATCGCCCAATCCAGACGTGGCTCTCATCGACGAAGCCGACTCCGTGCTGGTCGACGAGGCGC
    TGGTGCCCCTGGTGCTGGCCGGAACCACACATCGTGAGACGCCGCGGCTGGAGATCATCCGGCTGGTCGC
    TGAGCTTGTTGGCGACAAGGACGCCGACGAGTACTTTGCCACCGATTCCGATAACCGCAATGTCCACTTG
    ACCGAGCACGGGGCACGCAAAGTCGAGAAAGCGCTCGGTGGCATCGACCTGTACTCCGAGGAGCACGTCG
    GCACCACACTGACTGAGGTCAATGTCGCGCTGCACGCGCATGTGCTCCTGCAACGCGACGTGCACTACAT
    CGTCCGCGACGACGCGGTGCACCTGATCAACGCGTCGCGTGGCCGTATCGCGCAACTGCAGCGCTGGCCG
    GACGGGTTGCAAGCTGCGGTCGAGGCCAAGGAAGGTATCGAGACCACGGAAACTGGGGAAGTGCTCGACA
    CCATCACGGTGCAGGCCCTGATCAACCGGTATGCGACTGTGTGCGGAATGACGGGAACCGCGCTGGCCGC
    CGGTGAGCAGCTACGGCAGTTCTACCAGCTCGGTGTCTCACCGATACCACCGAACAAGCCAAACATCCGC
    GAGGACGAGGCCGACCGGGTCTACATCACCACTGCAGCCAAGAACGACGGGATCGTCGAGCACATCACCG
    AGGTGCACCAGAGGGGGCAGCCTGTGCTGGTCGGTACCCGCGACGTGGCCGAATCCGAGGAACTGCACGA
    ACGCCTGGTGCGCCGCGGTGTGCCCGCCGTGGTGCTCAACGCGAAGAACGACGCCGAGGAGGCCCGGGTC
    ATCGCCGAGGCCGGCAAATACGGCGCGGTCACGGTGTCAACTCAAATGGCCGGGCGCGGCACCGACATCA
    GGCTCGGCGGGTCCGACGAAGCTGACCACGACAGGGTCGCGGAATTGGGCGGCCTGCACGTGGTCGGCAC
    TGGCCGTCACCACACCGAGCGGCTAGACAACCAGCTGCGCGGTCGGGCCGGGCGCCAGGGAGATCCCGGG
    TCGTCGGTGTTTTTCTCAAGCTGGGAAGACGATGTCGTTGCGGCCAACCTCGACCACAACAAGCTGCCGA
    TGGCAACCGACGAAAATGGCCGGATTGTCAGCCCGAGGACGGGTAGTCTGCTCGACCATGCCCAGCGCGT
    TGCCGAGGGCCGGTTATTGGATGTGCACGCCAACACGTGGCGCTACAACCAGCTGATCGCCCAGCAGCGC
    GCCATCATCGTCGAACGGCGTAACACGTTGTTGCGCACCGTAACCGCGCGTGAGGAACTCGCCGAACTGG
    CGCCTAAGCGGTACGAGGAGCTGTCCGACAAAGTATCCGAGGAACGCCTCGAGACGATTTGTCGGCAGAT
    CATGCTGTATCACCTCGACCGTGGCTGGGCCGATCACCTGGCGTATCTGGCCGACATCCGGGAGAGCATC
    CATCTACGCGCGCTGGGCCGGCAGAACCCACTCGACGAGTTTCACCGGATGGCTGTGGACGCGTTCGCGT
    CGCTGGCCGCCGACGCCATCGAGGCGGCTCAACAGACGTTCGAAACCGCGAACGTCCTTGACCACGAGCC
    GGGGCTGGACCTGTCCAAACTGGCCCGGCCGACGTCGACATGGACCTACATGGTCAATGACAACCCACTG
    TCCGATGACACGCTTTCTGCCCTCAGTCTGCCCGGGGTGTTCCGCTG
  • Protein Sequence : Show Sequence
    >gi|15608958|ref|NP_216337.1| preprotein translocase subunit SecA [Mycobacterium tuberculosis H37Rv]
    MNVHGCPRIAACRCTDTHPRGRPAFAYRWFVPKTTRAQPGRLSSRFWRLLGASTEKNRSRSLADVTASAE
    YDKEAADLSDEKLRKAAGLLNLDDLAESADIPQFLAIAREAAERRTGLRPFDVQLLGALRMLAGDVIEMA
    TGEGKTLAGAIAAAGYALAGRHVHVVTINDYLARRDAEWMGPLLDAMGLTVGWITADSTPDERRTAYDRD
    VTYASVNEIGFDVLRDQLVTDVNDLVSPNPDVALIDEADSVLVDEALVPLVLAGTTHRETPRLEIIRLVA
    ELVGDKDADEYFATDSDNRNVHLTEHGARKVEKALGGIDLYSEEHVGTTLTEVNVALHAHVLLQRDVHYI
    VRDDAVHLINASRGRIAQLQRWPDGLQAAVEAKEGIETTETGEVLDTITVQALINRYATVCGMTGTALAA
    GEQLRQFYQLGVSPIPPNKPNIREDEADRVYITTAAKNDGIVEHITEVHQRGQPVLVGTRDVAESEELHE
    RLVRRGVPAVVLNAKNDAEEARVIAEAGKYGAVTVSTQMAGRGTDIRLGGSDEADHDRVAELGGLHVVGT
    GRHHTERLDNQLRGRAGRQGDPGSSVFFSSWEDDVVAANLDHNKLPMATDENGRIVSPRTGSLLDHAQRV
    AEGRLLDVHANTWRYNQLIAQQRAIIVERRNTLLRTVTAREELAELAPKRYEELSDKVSEERLETICRQI
    MLYHLDRGWADHLAYLADIRESIHLRALGRQNPLDEFHRMAVDAFASLAADAIEAAQQTFETANVLDHEP
    GLDLSKLARPTSTWTYMVNDNPLSDDTLSALSLPGVFR
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Vaccination of mice and guinea pigs with a secA2 mutant significantly increased resistance to M. tuberculosis challenge compared with standard M. bovis bacille Calmette-Guérin vaccination (Hinchey et al., 2007). In another study, lysine auxotrophy combined with deletion of the SecA2 gene made a live attenuated vaccine for Mycobacterium tuberculosis. SCID mice was highly protected from challenge after the vaccination (Hinchey et al., 2011).
  • Related Vaccine(s): M. tuberculosis secA2 mutant , Mycobacterium tuberculosis lysA/secA2 mutant vaccine
40. sigE
  • Gene Name : sigE
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 888751
  • NCBI Protein GI : 15608361
  • Locus Tag : Rv1221
  • Genbank Accession : BX842575
  • Protein Accession : NP_215737
  • Taxonomy ID : 83332
  • Gene Starting Position : 1364412
  • Gene Ending Position : 1365185
  • Gene Strand (Orientation) : ?
  • Protein Name : RNA polymerase sigma factor SigE
  • Protein pI : 4.82
  • Protein Weight : 27091.02
  • Protein Length : 257
  • Protein Note : Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; in M. tuberculosis this protein is involved in heat shock, oxidative stress and virulence
  • DNA Sequence : Show Sequence
    >gi|57116681:1364412-1365185 Mycobacterium tuberculosis H37Rv, complete genome
    CATGGAACTCCTCGGCGGACCCCGGGTTGGGAATACGGAATCGCAACTTTGCGTTGCCGACGGTGACGAC
    TTGCCAACTTATTGCAGTGCAAATTCGGAGGATCTCAATATCACGACCATCACGACCTTGAGTCCGACCA
    GCATGTCTCATCCCCAACAGGTCCGCGATGACCAGTGGGTGGAGCCGTCTGACCAATTGCAGGGCACCGC
    CGTATTCGACGCCACCGGGGACAAGGCCACCATGCCGTCCTGGGATGAGCTGGTCCGTCAGCACGCCGAT
    CGGGTGTACCGGCTGGCTTATCGGCTCTCCGGCAACCAGCACGATGCCGAAGACCTGACCCAGGAGACCT
    TTATCAGGGTGTTCCGGTCGGTCCAGAATTACCAGCCGGGCACCTTCGAAGGCTGGCTACACCGCATCAC
    CACCAACTTGTTCCTGGACATGGTCCGCCGCCGGGCTCGCATCCGGATGGAGGCGTTACCCGAGGACTAC
    GACCGGGTGCCCGCCGATGAGCCCAACCCCGAGCAGATCTACCACGACGCACGGCTGGGACCTGACCTGC
    AGGCTGCCTTGGCCTCGCTGCCGCCGGAGTTTCGTGCCGCGGTGGTGCTGTGTGACATCGAGGGTCTGTC
    GTACGAGGAGATCGGCGCCACACTGGGCGTGAAGCTCGGGACGGTACGTAGCCGGATACACCGCGGACGC
    CAGGCACTGCGGGACTACCTGGCAGCGCACCCCGAACATGGCGAGTGCGCAGTTCACGTCAACCCAGTTC
    GCTG
  • Protein Sequence : Show Sequence
    >gi|15608361|ref|NP_215737.1| RNA polymerase sigma factor SigE [Mycobacterium tuberculosis H37Rv]
    MELLGGPRVGNTESQLCVADGDDLPTYCSANSEDLNITTITTLSPTSMSHPQQVRDDQWVEPSDQLQGTA
    VFDATGDKATMPSWDELVRQHADRVYRLAYRLSGNQHDAEDLTQETFIRVFRSVQNYQPGTFEGWLHRIT
    TNLFLDMVRRRARIRMEALPEDYDRVPADEPNPEQIYHDARLGPDLQAALASLPPEFRAAVVLCDIEGLS
    YEEIGATLGVKLGTVRSRIHRGRQALRDYLAAHPEHGECAVHVNPVR
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A sigE mutant is highly attenuated in mice and induced significant protection from challenge with wild type M. tuberculosis, more so than the existing vaccine BCG (Hernandez et al., 2010).
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
41. sigF
  • Gene Name : sigF
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis CDC1551
  • NCBI Gene ID : 922492
  • NCBI Protein GI : 15842877
  • Locus Tag : MT3385
  • Genbank Accession : AE000516
  • Protein Accession : NP_337914
  • Taxonomy ID : 83331
  • Gene Starting Position : 3663004
  • Gene Ending Position : 3663789
  • Gene Strand (Orientation) : -
  • Protein Name : RNA polymerase sigma factor SigF
  • Protein pI : 6.53
  • Protein Weight : 26809.27
  • Protein Length : 261
  • Protein Note : Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; this sigma factor is a general stress response regulator; expressed in stationary phase and under nitrogen depletion and cold shock
  • DNA Sequence : Show Sequence
    >gi|50953765:3663004-3663789 Mycobacterium tuberculosis CDC1551 chromosome, complete genome
    GCTACTCCAACTGATCCCGTAGCCGTGCCAATGACTTGGCCAGCAGCCGCGACACGTGCATCTGTGAGAT
    ACCGACGCGCTCGGCGATCTGCGTTTGGGTCATCGAGTCGAAGAACCTGAGCACCAAGACCGTTCGTTCC
    CGCTCGGGCAACGCCTCGAGCAACGGACGAAGCACCTCCCGATTCTCGATCTGGTCAAGACCCGCATCCA
    CGTCGCCCAGGGTGTCTGTGATTGCGCGGGCATCGTCGTCGCTGCCGCCACCGCTGTCGATGGACAAGGT
    GTGGTAGGAACTACCCGCCAGCAAACCTTCGATAACCTCAGCGCGGTCCATCCCGAGCTCCGCGGCGAGC
    TCCGATGCCGACGGCGCCCGCCCGAGCCGCTGCGACAAATCGGCGGTGGCGGTACCTAGCCGCAGATGCA
    GTTCCTTGAGACGCCGGGGAACCTTGACCGACCAGCTGTTGTCGCGGAAGTGTCGTCGGACCTCGCCCAT
    GATGGTAGGAACCGCGAAGGAGACGAAGTCCGACCCGGTCTTCACGTCGAAGCGAACCGCGGCGTTGACC
    AGCCCGACCCGCGCGACCTGAATAAGGTCGTCACGCGGTTCGCCGCGACCCTCGAACCGCCGCGCGATGT
    GATCGGCCAGCGGCAAGCACCGCTGAACGATCTTGTCCCGGTGCCGCTGGAATTCCGGTGAGCCGGCAGG
    CAAACCAACCAGCTCGCGAAACATCTCCGGAACGTCGGCGTATTCGTTAGCTCGCGATGCAGAACCGCCG
    GCAGCGCGCGCCGTCA
  • Protein Sequence : Show Sequence
    >gi|15842877|ref|NP_337914.1| RNA polymerase sigma factor SigF [Mycobacterium tuberculosis CDC1551]
    MTARAAGGSASRANEYADVPEMFRELVGLPAGSPEFQRHRDKIVQRCLPLADHIARRFEGRGEPRDDLIQ
    VARVGLVNAAVRFDVKTGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGTATADLSQRLGR
    APSASELAAELGMDRAEVIEGLLAGSSYHTLSIDSGGGSDDDARAITDTLGDVDAGLDQIENREVLRPLL
    EALPERERTVLVLRFFDSMTQTQIAERVGISQMHVSRLLAKSLARLRDQLE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A sigF mutation is highly attenuated in Mycobacterium tuberculosis and induced protection from challenge in mice(Geiman et al., 2004).
42. SodA
  • Gene Name : SodA
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 886174
  • NCBI Protein GI : 15610982
  • Locus Tag : Rv3846
  • Genbank Accession : NC_000962.2
  • Protein Accession : NC_000962.2
  • Other Database IDs : EMBL: AE000516
    EMBL: AF061030
    EMBL: BX842584
    EMBL: X52861
    Gene3D: G3DSA:3.90.149.10
    GenomeReviews: AE000516_GR
    GenomeReviews: AL123456_GR
    InterPro: IPR001189
    KEGG: mtc:MT3960
    KEGG: mtu:Rv3846
    LinkHub: P17670
    PANTHER: PTHR11404
    PDB: 1GN2
    PDB: 1GN3
    PDB: 1GN4
    PDB: 1GN6
    PDB: 1IDS
    Pfam: PF00081
    Pfam: PF02777
    PIR: S15205
    PRINTS: PR01703
    ProDom: PD000475
    PROSITE: PS00088
    TIGR: MT3960
    TubercuList: Rv3846
    UniProt: P17670
  • Taxonomy ID : 1773
  • Gene Starting Position : 4320704
  • Gene Ending Position : 4321327
  • Gene Strand (Orientation) : +
  • Protein Name : SUPEROXIDE DISMUTASE [FE] SODA
  • Protein pI : 6.43
  • Protein Weight : 23033.8
  • Protein Length : 207
  • Protein Note : Rv3846, (MTCY01A6.22c), len: 207 aa. sodA (alternate gene names: sodB, sod), superoxyde dismutase (EC 1.15.1.1) (see citations below), equivalent to many e.g. P47201|SODM_MYCAV|SODA|SOD from Mycobacterium avium (206 aa), FASTA scores: opt: 1210, E(): 1.8e-73, (82.5% identity in 206 aa overlap); Q9F9R1|SOD from Mycobacterium paratuberculosis (207 aa), FASTA scores: opt: 1207, E(): 2.9e-73, (81.65% identity in 207 aa overlap); O86165|SODM_MYCLP|SODA|SOD from Mycobacterium lepraemurium (206 aa), FASTA scores: opt: 1204, E(): 4.5e-73, (82.05% identity in 206 aa overlap); P13367|SODM_MYCLE|SODA|ML0072 from Mycobacterium leprae (206 aa), FASTA scores: opt: 1169, E(): 9.6e-71, (80.5% identity in 205 aa overlap); etc. Contains PS00088 Manganese and iron superoxide dismutases signature. BELONGS TO THE IRON/MANGANESE SUPEROXIDE DISMUTASE FAMILY. ALTHOUGH FOUND EXTRACELLULARLY, NO SIGNAL SEQUENCE IS PRESENT. AN ALTERNATIVE SECRETORY PATHWAY MAY BE USED.; sodB; sod
  • Protein Annotation : FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems (UniProt: P17670)

    CATALYTIC ACTIVITY: 2 superoxide + 2 H(+) = O(2) + H(2)O(2) (UniProt: P17670)

    COFACTOR: Binds 1 iron ion per subunit (UniProt: P17670)

    SUBUNIT: Homotetramer (UniProt: P17670)

    SUBCELLULAR LOCATION: Secreted protein (UniProt: P17670)

    SIMILARITY: Belongs to the iron/manganese superoxide dismutase family (UniProt: P17670)

    CAUTION: Although found extracellularly, no signal sequence is present. An alternative secretory pathway may be used (UniProt: P17670)
  • DNA Sequence : Show Sequence
    >GeneID|886174 [Mycobacterium tuberculosis H37Rv ] 4320704..4321327
    gtggccgaatacaccttgccagacctggactgggactacggagcactggaaccgcacatctcgggtcaga
    tcaacgagcttcaccacagcaagcaccacgccacctacgtaaagggcgccaatgacgccgtcgccaaact
    cgaagaggcgcgcgccaaggaagatcactcagcgatcttgctgaacgaaaagaatctagctttcaacctc
    gccggccacgtcaatcacaccatctggtggaagaacctgtcgcctaacggtggtgacaagcccaccggcg
    aactcgccgcagccatcgccgacgcgttcggttcgttcgacaagttccgtgcgcagttccacgcggccgc
    taccaccgtgcaggggtcgggctgggcggcactgggctgggacacactcggcaacaagctgctgatattc
    caggtttacgaccaccagacgaacttcccgctaggcattgttccgctgctgctgctcgacatgtgggaac
    acgccttctacctgcagtacaagaacgtcaaagtcgactttgccaaggcgttttggaacgtcgtgaactg
    ggccgatgtgcagtcacggtatgcggccgcgacctcgcagaccaaggggttgatattcggctga
  • Protein Sequence : Show Sequence
    >gi|15610982|ref|NP_218363.1| SUPEROXIDE DISMUTASE [FE] SODA [Mycobacterium tuberculosis H37Rv ]
    MAEYTLPDLDWDYGALEPHISGQINELHHSKHHATYVKGANDAVAKLEEARAKEDHSAILLNEKNLAFNL
    AGHVNHTIWWKNLSPNGGDKPTGELAAAIADAFGSFDKFRAQFHAAATTVQGSGWAALGWDTLGNKLLIF
    QVYDHQTNFPLGIVPLLLLDMWEHAFYLQYKNVKVDFAKAFWNVVNWADVQSRYAAATSQTKGLIFG
  • Related Vaccine(s): M. tuberculosis DNA vaccine pAK4-sod
43. trpD
  • Gene Name : trpD
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 887681
  • NCBI Protein GI : 15609329
  • Locus Tag : Rv2192c
  • Genbank Accession : BX842579
  • Protein Accession : NP_216708
  • 3D structure: PDB ID : 2BPQ
  • Taxonomy ID : 83332
  • Gene Starting Position : 2455630
  • Gene Ending Position : 2456742
  • Gene Strand (Orientation) : -
  • Protein Name : anthranilate phosphoribosyltransferase
  • Protein pI : 6.4
  • Protein Weight : 34267.71
  • Protein Length : 370
  • Protein Note : Catalyzes the conversion of N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate
  • DNA Sequence : Show Sequence
    >gi|57116681:2455630-2456742 Mycobacterium tuberculosis H37Rv, complete genome
    GTCAGATCTGCCGACCGAACCGCACCCATCGCGCGAGCAACTGTTCGGCCGCACCGGTGTCGATTGCCGC
    ACTGGCCCGCCGCAACCCCTCCTCCCAGGCCGGCAGCCATTCAGCACGGCTGGATAATCCGGCGTGGGCG
    ACGATCGCACCGGCGGCGTTGAGCACCACAGCGTCCCGGACCGGGCCCCTGGCACCGCCCAACACCGCGC
    GCACCGCGGCCGCGTTGGCTTGCGCATCGCCTCCAGCCAGCTGGTCAAGCTGGGCGCGCGCAAACCCGAA
    TCCGGCGGGATCAAACGTCAACTTATCCACGCTGCCCGCCGCAACGCGCCAGATCGTGCTCGTGGTGGTG
    GTGGTCAACTCGTCCAGCCCATCGTCGCCGTGTACCACCAGCACACTGGACCGGCGCGCAGCAAACACCC
    CGGCCATCACTTCGGCGAGGTCGGCGAACGCGCATCCGATCAGTCCAGCCCGGGGCCGGGCCGGATTGGT
    CAGCGGCCCGAGAAGATTGAACACGGTGGGCACACCGATCTCGCGGCGTACCGCGGCCGCGTGCCGGTAG
    GAGGGATGGAACCGCGGCGCGAAGCAGAACCCGATCCCAACCTCCGCGAGGCTGCGCGCGACCAGGTCGG
    GTCCCAGGTCGATGCGCACCCCCAGCGCCTCCAGCGTGTCGGCGCCACCGGACAACGAGGACGCCGCTCG
    GTTGCCGTGCTTGACCACCGGCACACCCGCAGCCGCCACCACAATCGCCGCCATGGTGGATAGGTTCACC
    GTGTTGACTCCGTCGCCACCGGTGCCGACGACGTCGACGGCGTCGTCGGGGACCGTATCGGCGGGCAACG
    GATGCGCGTGGCTGAGCATGACGCCAGCGAGCTCACCGACTTCGTCGGCGGTCGGAGCCTTCATCGTCAT
    CGCCACCGCGAAGGCGGCGATCTGCGCCGGCCGCGCATTGCCGGTCATGATCTGGTCCATGGCCCAGGCA
    GCCTGGCCCCGCGCCAGATCGCGGTTGTCGGTCAACCGCCCCAAAATCTGCGGCCAGGACGGCACCGATG
    CGGCTTCTGCTTTCGGCGAGCCCCCGCGAGATCCCCCCGAAGAACCCTCAGCTGACAGCGCCA
  • Protein Sequence : Show Sequence
    >gi|15609329|ref|NP_216708.1| anthranilate phosphoribosyltransferase [Mycobacterium tuberculosis H37Rv]
    MALSAEGSSGGSRGGSPKAEAASVPSWPQILGRLTDNRDLARGQAAWAMDQIMTGNARPAQIAAFAVAMT
    MKAPTADEVGELAGVMLSHAHPLPADTVPDDAVDVVGTGGDGVNTVNLSTMAAIVVAAAGVPVVKHGNRA
    ASSLSGGADTLEALGVRIDLGPDLVARSLAEVGIGFCFAPRFHPSYRHAAAVRREIGVPTVFNLLGPLTN
    PARPRAGLIGCAFADLAEVMAGVFAARRSSVLVVHGDDGLDELTTTTTSTIWRVAAGSVDKLTFDPAGFG
    FARAQLDQLAGGDAQANAAAVRAVLGGARGPVRDAVVLNAAGAIVAHAGLSSRAEWLPAWEEGLRRASAA
    IDTGAAEQLLARWVRFGRQI
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A trpD mutant is highly attenuated in mice and induces more protection from challenge with wild type M. tuberculosis than that induced by the current vaccine BCG (Smith et al., 2001).
  • Related Vaccine(s): Mycobacterium tuberculosis trpD mutant vaccine
1. APOBEC3B
  • Gene Name : APOBEC3B
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 9582
  • NCBI Protein GI : 22907025
  • Locus Tag : CTA-150C2.15-003
  • Genbank Accession : AL022318
  • Protein Accession : NP_004891
  • Taxonomy ID : 9606
  • Chromosome No : 22
  • Gene Starting Position : 22346831
  • Gene Ending Position : 22357253
  • Protein Name : apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B
  • Protein pI : 5.94
  • Protein Weight : 44235.85
  • Protein Length : 382
  • Protein Note : Also known as ARP4; ARCD3; PHRBNL; APOBEC1L; bK150C2.2; DJ742C19.2; FLJ21201
  • DNA Sequence : Show Sequence
    >gi|157729478:22346831-22357253 Homo sapiens chromosome 22, alternate assembly HuRef, whole genome shotgun sequence
    CACAGAGCTTCAAAAAAAGAGCGGGACAGGGACAAGCGTATCTAAGAGGCTGAACATGAATCCACAGATC
    AGGTACCGCTGCCCACTCTGCCTGCTGGGCCCCTCCTGCCCCTTCCTGCCTGGTGGTCCTGCTGGGCCTC
    AACCCTGGCCTCCCCCCGCCCCTGCCCCAGCCCTGGGCTCCCTCCCCTCTGGCTCCCCTGCCACACGAAT
    CCCAGTCAGGCTCTTTGTCCTGCTGTGTGGTCACCCCACTGCTGCTTCTGAATGGGCTGCCTCCCCTACC
    TGCACCAGCCCAGGCCCCGTGCTGAGACTCTCCCTTAAAGTCATGCCCGGACTGGTGCTCCCCGCCCTGG
    GAGGGTGCTCCCTCTGTGTGCTTCCTGCCACTCCTGAGCTCAGAAACTGGGAGAATTGAACCAAAGGGTA
    ATTGGAGCAATCAGGCATTTTTGTTCTCAGTGTTTTGATGAAATTTCTGTAAGAATTACTTTGCTTAAAT
    ACAAAGTCTTAGGAGAGGGTGCGGGGGTAGGAATGGTCTCTGAAGCACATGATAAATCGTGTCATCCAAG
    GATGACTCCATGGTGAGCAGATGTCCCCAGACAGGTTCCACTCCAAGGTCACTCTTCATCTTTTAGAACA
    TGACCAGTGGTTGAGCGTGGTGGCTCACACCTGTAATCCCAACACTTTGGGAGGCCAAGGCGGGCGGATC
    ATGAGGTCGGGAGTTTGAGACCAACCTGGCCCATATGGTGAAACCCCGTCTCTACTAAAAAACATACAAA
    AATTAGCCAGGCATGGTGGCGCATCTCTAGTCCCAGATACTCAGGAGGTTTAGGCAGGAGAATTGCTTGA
    ACCTGGGAGGTGGAGGTTGCAGTGAGCTGAGATCATGCCACTGCACTCCAGCCTGGGCGACAGAGTGAGA
    CTTCATCAAAAAAAAAAAGAAAGAACATGATCAGTAACATGGAATGTGAGAATGTGGGAAAAAAGTGCAA
    TGATGAGGAGAAATGCTCTGATTTTAAAATTTAGTGAAAAGAGAGCCCAGTCCCCAACAGCTTTCTCCTA
    CAGGATCCGTGATGCAGAGGCTGGACAGGGCTGGCCTGGAAAGGGGCCCCTGCTCCATCCGTCCCCAGCT
    CTGTGGCCTCGGCAGGTTACCCCGCCTCTCTGTGCCTCTGGCATCTCCTCTGTAAGATGGGAATGGCCCC
    TGCAGGCCTAGGGTAGCCTCACGTGAGCTCACACCCGCTCAGCACTTAGAAGAGTGGCCTGGGCCTCAGA
    ATTCAGTTCTACCATGATTTTAAAATAATGTTAACACGCAGAGTGAAACATCAGGAAGGAATTGAGCTGA
    AAGGTTAAAAGCTATTTTTTAGGGGTGAAGGGTTTTATTCTCTTTTGCATTCTCCTAATGGGTTGGGGAG
    TGGGTTGGCAGAAATACTAGGAGGGTGGGGAATGTACCCCTGGAAAGGCCAGAGTTGGACCCGAGCTGGG
    AGAGATCACCCCAGCCCGCCTGCCAGCATCCCCTCCTCTTCCTCTCCCCTCAGTCTTCCTGCCTGGGAAG
    GCAGCAGAAATTCTCAGGGCTGTGGAGGGGTCGGGGAGGCCCAGGGCCATCCTGGGAGCTGTGTTCAGTG
    GACATGAGCCCCGAGGACTCCCGGGAGGGCTCCCTGCATGGGCCGGTTTCTCTCTTGTGCCTTCAGAAAT
    CCGATGGAGCGGATGTATCGAGACACATTCTACGACAACTTTGAAAACGAACCCATCCTCTATGGTCGGA
    GCTACACTTGGCTGTGCTATGAAGTGAAAATAAAGAGGGGCCGCTCAAATCTCCTTTGGGACACAGGGGT
    CTTTCGAGGCCAGGTACCACCCAAACTTCAATCGAATCACAGGCAGTGTTGCAGGAATTAGAGGACTGGA
    GAGACTGATATGGGTGAGACAGGAGGATTTATTTAGGTGCACTGGTTCAGCAGACTCACATCCAAAAAGG
    CTGAGCCCTTAACAAAGACAGACTGAGGTATTTATAAGCAAACTTACAGAAGCAGAACAAAGGCGATTAA
    TCAAACAGTGACAGGTCACATAATCTATAGCATAACAGATGACTTAGCATAACTTGGGGCCTTCTATAGC
    TGGTGGCCTTGCAGCTACATCAAAAGGAAAACAGGAACTGGCTAAATACAGACATTTGTAAAATATAGTT
    ATAATGGTTCTGGAAAGGAGAGACAGTAAAGGAATTTGTCTTTTTTTTAAAAAATTTTTTTAAAATTTTC
    TTCAACCTTGCCCTGGAGGGGTGTGGGGGTGGTGTCTGGAGCCCATTCCTTTGGCCTTGACTTTTCAGGC
    AGTGTTATAACTGTCGTTGGAGTGAGCTGGCTAGGCAGAGGAAAACTTGTTCTTCTTTTCTTTTTAACCC
    TTGCTACAGTAGGACCTAAGCAGCTGGAAATGCAGAAAACACAACAATAAGTAAAATGCCCAGCACTGGG
    CTCTCAATTGTGTATTTTTCCCACATTTCTTTTTTCTCTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTCC
    GAGATGGAGTCTCGCTCTGGCACCCAGGTTGGAGTGCAATTGCACAATCTTGGCTCACTACAACCTCCAC
    CTCTCAGGTTCAAGTGATTCTTCGGCCTTAGCCTCCCGAATAGCTGGGATTACAGAAGGCTGCCACCACG
    CCCAGTTAATTTTTTCTATTTTTAGTTGAGACTGGGTTTCACCATGTTGGCCAGGCTGGGGAATTCCTGA
    CCTCAGGTGAGTCACCTGCCTTGGCCTCCCAAAGTGCTGGGGTTACAAGCATGAGTCACCGCACCTGGCC
    ACTTCCCACAGTTCTGTTTTTTTGTTTTGTTTTGTTTTGGTTTTTTTTTAAGACTGAGTTTCACTCTTTG
    TTGCCTGGGCTGGAGTGCAATGACTCACTCTCAACTTCCACCTGCCAGGTTCAAGTGATTCTCCTGCCTC
    AGCCTGTGAGCTGAACAGTCACATGAGGGTGAAGGTTGTTGCCACAAGCTCAGGGGATGCTCCAGACAGA
    GTGGCCTGGGATGTCGAGTCACAGCTGCTCCATGCCACGGGGACAAGAGGAAGCAGGAGTTTAGTCTGAC
    ACACTGCCCTTCCAGATAGAGGGCAAGAGACAGAAAGAGGGGCTGAAGTCACCCTTGAATAACCACAGCA
    GTGGCACCCACAAAGGTGCAGTCCCCACAGCCTCATAGCCTCTTCTAGGTGCCACCTCTTAAGGCCATTA
    CAATAGCCATTAACACTGAACGTGAGCTTTGGAGCAGACAATCACTCAAACTAAACAGGGGGGATGGAGG
    AAAGGAGCTTCAATGGCAAGATCCCCTGGGCTCCTGTCCTGGCCCCTCCTCTCCCTGCCCCACCCCTGCA
    CTCCTCCTGCTCCCCCTCTCAGAGCATCCCCTGCCCCCTGCTCCTCTCCCAGGTGTATTTCGAGCCTCAG
    TACCACGCAGAAATGTGCTTCCTCTCTTGGTTCTGTGGCAACCAGCTGCCTGCTTACAAGTGTTTCCAGA
    TCACCTGGTTTGTATCCTGGACCCCCTGCCCGGACTGTGTGGCGAAGCTGGCCGAATTCCTGTCTGAGCA
    CCCCAATGTCACCCTGACCATCTCCGCCGCCCGCCTCTACTACTACTGGGAAAGAGATTACCGAAGGGCG
    CTCTGCAGGCTGAGTCAGGCAGGAGCCCGCGTGAAGATCATGGACTATGAAGGTGAGAGGTGGAGGGGTC
    AGGGGAGCGTGAGCGGGAGGAACAGCATGAAAGATGGATGGATCTGCAATGCCATGGCTGGGGGTGTCCC
    AGGGCAGCCTGCAGGGGTGGGACTGGCACTGACTGCAACTGACAGCCAGGAGACCAGGCCTGGGAGGACA
    GGCCAGGGTCAGGGGAGAGCCTGACTGCTTCCCGCTTCTTCATCTCAGAATTTGCATACTGCTGGGAAAA
    CTTTGTGTACAATGAAGGTCAGCAATTCATGCCTTGGTACAAATTCGATGAAAATTATGCATTCCTGCAC
    CGCACGCTAAAGGAGATTCTCAGGTGAGGGTCTCCCTCTGGCCTCATCATCTCTCTCCTCTCACCTGCTC
    ATCCTCCTGAGGCCTCCGTTGGCCTGGCCCTCCCGCCCTCCCTCTTGACCCACTGCCTGCCCTCATGGTC
    ACACCACCTTCCCTTAACTCCTGGTGCTCCCTCCACACTGCCTCCTCCCTGCTTTCCTGGGCCCTTCCTG
    TGAGTAAGAGGCCCCTTCTGCCTCTAAGGCACCTGGGTCTTGGACTAGAGGAACAAACAGCAAGACAGGG
    TGGCCTGGGACACCAGCCGCTGCCCTTCTGTGACATGGGGACAAGAGGAGGCAAGAGCTCAGCCTTCCAG
    GGAACAACTCTGGGCAGGAGATGTGGAAGGAAGGAGCTCAGTGTGGGGGCACCCATGGCATCCTGGGGGG
    ACATCTGAGGGCCACCCCCACCCGCTATTCCTCCCTCCAATGGTGGCCTCTGAGTGTGAAGGCATGGGGG
    AAGGCAGACACCTGCCCCTGACTCTCCCTCACTACCACATAGCTGCTGGGTGGCTGGGGACGGGGGGGGT
    CCCTGGGATGATTCCTGAGGGCAGGATCCAGGGGTCCCTCCAGATGTCCTCCCATCCAGGTGAGAAAGCA
    GCATAAAGTGAGGCTTGCACTCAGATGGGCCTGGGAGGTCACTCACAGCGCAGGTGTCTCCTGGAACAAG
    GGCCCTGGAAGATAGAAATAGAATCCAAACCCAGGACTAACATCAGGGCCAAGACAAGCCTGCCAGGGAG
    GCTGCACATGAAGCCCCAGATCAGGGACCACTGCCCGCTCTGCCCATGGGGCCTCTGCTGCCCTTTCCTG
    CCTGGTGGTCCTGCTGGGCCTCAGCCCTGGCCTCCCCCTGTCCCAGCCCCAGCCCTGGGCTCTCTCCCCT
    CCTGTTCCTCTGCCACCCCCACTCCCAGCCAGGCTCTTTGCCCTGTTGTGTGGTCGCCCCACTGCTGCTT
    CTGAATGGGCCACCTCCCCCACCTGCCCCAGGCCAGACCCCCTGCTGAGACTCTTCCCTGAAAGTCACCG
    CTGGGCTGGTGCTCCCTGCCCTGGGAGGGTGCTCCCTGTATATGCTTCCCACCATTCCTGAGCTCTGGAA
    CTGGGAGAATTGAACCAAAGGATGATTGGAGCAATCAGGCATTTTGTTCTTAGCATTTTGATGGAATTTC
    TGTAAGATTTACTTTGTTTCAACACAAAGTCTTAGGAGAGGGTGTGGGGGAGGAAATGGTCTCTGTACCA
    GAAAATAAGTCATTTCTTCCAAAGACGCCTCCGCTGTGAGCAGAGGAGGATGCACACGACCCCAGACACA
    GGCTCCTCCTCCGTGAGCACCATTCACCTTTTAGAATGACACCTGCAACATGGAGTGTAGGAAAACATAT
    GATCAGGAGAAATGCTCTTATTTTAGGCAGGGCACGGTGGCTTATGCCTATAATCCTAGCTCTTTGGGAG
    GCTGAGGTGGGTGGATCACTTGAGGTCAGGAGTTGGAGATCTGCCTGGCCAGCGTGGCAAAACCTCCTCT
    CTACTGAAAATACAAAAAATGAGCCAGGCATGGTAGCGGGAGCCGGTAATCCCAGCTACTTGGGAGGCTG
    AAGCAGGAGAATCGCTTGAACCCGGGAGGGACAGCAGTGCTGGTCACGGAAACACCCAGGACTCAGGACT
    GAAGCACAAGTGTTTCCAGTCCCCACACACTAAAAAGGAGACAAGAACTCCTCTTCGAGCTTTTCTGATG
    AGCACTCACCTCTCACCCTCAAACCCCTGGGGCCTCTCTTTTCCTCCTGACCCTCTCTCTGGACCTGGCC
    TCTTGCTCTAGGTTCCCAGTTTTGGTCCCAGTGCTGGTCTCTCCTCCAATGGTACCAATTCCAGGTCTCC
    TTCCACCTCCTGGGGCAGATGCACAGGGAGCCTGAAAATCCCAGTGGATAATGATGCTGTGCCCCAGCCA
    GTGAAGACAGTTGGAGAGGGGATTCTCTTCACTTGTCTTTAAATGAGCATCTACTATTTCTTGCAAATGT
    TCCATGTCTCTGGACTTGGCCTAAGCCATTTTTATGTTCAGTGTCATCTCGTTCTCCCTAATATTTAGAA
    AAGGGTGTTGCTTGTAGGACCACTTTGCTATGAAGAAGCCTTGTTTCCTTTTCTTGAGAACACAAAGCAA
    GTGAGTGGTAGAGCCCAGATTCATACCCAGGTCTACTAGAAATTGTGGCAAACAGAGATTCCTTCCTTCC
    TTCCTTCCTTCCTCCCTTCCTTCCTTCCTTCCTTCCTTGCTTCCTCTCTCTTTCTCTCTTTCTCTCTTTC
    TCTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTT
    TCTTTCTTTCTTCTCTCTCGTCTTTCTTCTTTTGCTGCTGCCTCCAAACAGAGATTTTTCTAATTCTGGT
    TTTGTCAGGATGTCTACAGAGGGGCCGGGAGAGGCCTTCAAGGTGGGGCTGGTGTTTCCAGCCCAGGAGT
    CCTGAGCTGCCCCATCTGGCCTGAGAAGCACGTTTGGGAGACCTCATGGCATCCAAAGGGACGTGGAAAA
    TGCTATGAATTATCCAAAAAGATGTCCCTGGCAGGCTCCTCTCCCCAATGATCCCCCTCAGGAGTGTCCC
    TGCCCCAATGCTGGGTGTGGTAGGAGTTAACCCTGCAGGCAGGAGGAAGCCCCAGCAGGTCCATCTCCAG
    CAGGGGCTGTGGGTGAGCAGAGCCAGGACAGGGGTGCATGGTGAGGCCAGGGAAGGAGACCCAGCTCTAC
    CCCAGGGAGAGGGGCAGGGTCCTCCCCGGAGGGCCTGAGCACACTGAGCTGACCCTGGGGAGACCCTGAC
    AAGGCCTAAACAGGCCCCGGGGCTGTGTTGACTTCCTGATAATCCACCAGAAGGGGTCAGAGAGAGAGGA
    TCAGTGGCCCCCACAAAGGGAGTGGAAGCGCCTCCTCTGACAGGTGCCTCAGTATATGGGGAGCAGGGAA
    GGAGTGGGGGGTGCAGGAGAGGAGCGAGAGGTAGTCCCAGGAGGAGTCTTAGGGCTTTTGGTTTCCCCTG
    TCTTTGTCCACAGATACCTGATGGATCCAGACACATTCACTTTCAACTTTAATAATGACCCTTTGGTCCT
    TCGACGGCGCCAGACCTACTTGTGCTATGAGGTGGAGCGCCTGGACAATGGCACCTGGGTCCTGATGGAC
    CAGCACATGGGCTTTCTATGCAACGAGGTGACCGACCCAGCCACCTGCATCCAGGCAGGGCCCTCCCAAT
    CCAGGGACACTCATAGATAGAAGGTTCTGGGTGGTGCCTGTGGTTTCCTGCAGTGTTTGTCACTTGTGCT
    TCCTGCAGCTGCTGCTGCTTGGCCCTGGAGTTGGGGGGAGACTTTGGCTTCAGTGACTCTCCATGGCCAG
    GTGGGGTTGACTCTGCCCAATGGCAAAGTGCTTCCTGAGGACCCTCCCAGGATCCCCTCACAGACACAGC
    TCTCACCAGGAATGATTCGGAACTGTGGGATTTGAGGACTCAGGGCCTACCTGACTCACAAGGCCAGGAT
    GTCCCTGTGCCCTCTCTTGGGCTTCATCCTGCGGAGAGACAGACTGGGGCAGGAGAGGCTGATCAGGGGT
    TTTGTCCTGCCCAGGGTGGAGCCCACAGCAAGGCCAGAACAGGTCCCATGTCAGGATGCAAAGATGGCCA
    GCAGTTGGGGAAGAACTGGGCCAGGAAAGGCTGAGGGGGCCAGGAAAGGCTGAGGGGGCCAGGCTAGGCT
    GGCCAGGAGCCCTGAGCCCAAGGGACTTTCTTCCCTGGCCCCTACCCAGCACAGCCTCTGTCTGGAGAGG
    CCAAGTTCTGCTTGGCTCTGTTGCTGGAAACTGGGGGCTTCTCTGGGCTCTATGAGCTCAATGTGGGCCT
    CGCTGGATCCACACACACTGCTTGGAACATCCTTTCAAGGGTGCCAGTCTCCATCACCGCCTAAGCAGTG
    GGACTCCCCCAGGAATGACCCACAGCCCCTTCTCAGCACAAACCATGTCATCCACTTCACCCTGCATCCC
    CTCTGATGGAATCCCTCCCTGTCTTTGTCCCCATCACAATCACAGTCCTTGCTGCCCTGCTGTCCCCAAC
    TTGACCGATTCCTAGTCTTAGTGGAAAATCCTTTTCTCTGAAGTTTGGAGTAAATACCATATTTCTATAA
    GTCAAAATAATGACAATTATACCATGTCACAGGGACCTTCCCACATTGGAGTGAGTCCTGCTGGTCTTCT
    CTTCCCCACTACTTTTGGTGGAATTCATTTTGGCCTAAGTCTATATCACATTTGGGCTCAGCACAGGTTG
    TGGCACAGAATAGAAGTTTCTAGAATTATGGGCCACAGATGGGATGGGAGTAGGGGAGTTGATGAGTAGA
    GCAGGTCAATCTCCCCTTGAAGGAAGCACACTCACTCAGGGGCATCAGGCCAATGACCTTGGGGCTCCGC
    CGGCCCCTCCTCCCTGCCCCCATCTCTGTAGCCCCTCCCTGCAGGGCTGGGTCTGGGGTGAGGGTCCTGA
    AGGCTCTGACCTTGGGTACAAAATTGATGGGGGCTCCAAATGCTCAGTAATTAGAGAAAACACATTTTAA
    TTTAATAATTAATGCAATATTTTAAAACGCAAATTTAATACAAATAGATCATGATGAATAAAAGCAAAGA
    TTTTAATAAAGCCTGGCAGGAGAGTGCTGTCCTGTGTATATTGGAGCTGGGGCAAAATAAAACCTCATTG
    CTCCTGATCTGTTTGTATTTAAATGGGCAACATTTTGCTTATCTTGGGATTTTTGCATAACATCTGATAT
    TTTTAATATTGCATAGAAAATCATGCATCCTAATAACCGGGGTTTTGGCGACCCCTCTAATTTCGTGCTG
    GAGGCCCCCGCCTCCCCAGCCCCACCTGGTCCAGGCGCTCCCTCCCTGTTCACCACACATCACCTCACAC
    TCTGTTTCCTTTTCTAGGCTAAGAATCTTCTCTGTGGCTTTTACGGCCGCCATGCGGAGCTGCGCTTCTT
    GGACCTGGTTCCTTCTTTGCAGTTGGACCCGGCCCAGATCTACAGGGTCACTTGGTTCATCTCCTGGAGC
    CCCTGCTTCTCCTGGGGCTGTGCCGGGGAAGTGCGTGCGTTCCTTCAGGAGAACACACACGTGAGACTGC
    GCATCTTCGCTGCCCGCATCTATGATTACGACCCCCTATATAAGGAGGCGCTGCAAATGCTGCGGGATGC
    TGGGGCCCAAGTCTCCATCATGACCTACGATGGTAAGAATGGAAGGTTCAGGTGGGGTGGGGTGGGTGGG
    GGCAGGAGAGGTTCCTGGGAAGAAAAGGAGAAAGGCCTTGGTCTGCTGCCTGCAGAAACGATGGCTGGAC
    TCTGGGACCTGACTTTGGGGTCGATGGGAAGAGAGAGGCCAGGCCAGGAGATATGGGCCCGGGGAGGGTG
    GCTGGAAGTGGAAGCAGAACTTGGGGATTTCCGAAAGAAAGAGAACTGGGCTGGCCCAGATTCCAATGGG
    AAGGAAGTACCTGATGAAGGAGCTAAGTCCCTAGGGGAGGGAGAGGGAAAGGAGGGACTGAAACCAGGAT
    GTGGGAAGTCTGTCCTGAGAGTCATGGGCCCTTGGTGCTGCCCCCTCCCCACAACAGGAGCGTGACTTAT
    CTCCCCTGTCCCTTTTCAGAGTTTGAGTACTGCTGGGACACCTTTGTGTACCGCCAGGGATGTCCCTTCC
    AGCCCTGGGATGGACTAGAGGAGCACAGCCAAGCCCTGAGTGGGAGGCTGCGGGCCATTCTCCAGGTGAG
    GGCTTCCTCCCTCTGCCTGGTGCCCCATCGGCCTCCCCCTCCTCCCCGCTCCCCTGTGCCTTGCCTTCCC
    CTCTGCTCAGAGCCTCCTCTGGGTTCCCTGCTCCCCACAGGGCGCCCAGCTCCGTCCCTCCCTTTCCTTC
    TCACAGCCTCCTTCTCTTTCCCACCTCCCGCATCCCTCCCTCCTCTCCCGTCATTGTCACTGTCCCCAGG
    CCACCTCCCTGTGCCCTCTTTCCACTCTCTCACCTCCTGCTCCATTCAACCCCCCTGCTCTTCCAGAATC
    AGGGAAACTGAAGGATGGGCCTCAGTCTCTAAGGAAGGCAGAGACCTGGGTTGAGCAGCAGAATAAAAGA
    TCTTCTTCCAAGAAATGCAAACAGACCGTTCACCACCATCTCCAGCTGCTCACAGACACCAGCAAAGCAA
    TGTGCTCCTGATCAAGTAGATTTTTTAAAAATCAGAGTCAATTAATTTTAATTGAAAATTTCTCTTATGT
    TCCAAGTGTACAAGAGTAAGATTATGCTCAATATTCCCAGAATAGTTTTCAATGTATTAATGAAGTGATT
    AATTGGCTCCATATTTAGACTAATAAAACATTAAGAATCTTCCATAATTGTTTCCACAAACAC
  • Protein Sequence : Show Sequence
    >gi|22907025|ref|NP_004891.3| probable DNA dC->dU-editing enzyme APOBEC-3B [Homo sapiens]
    MNPQIRNPMERMYRDTFYDNFENEPILYGRSYTWLCYEVKIKRGRSNLLWDTGVFRGQVYFKPQYHAEMC
    FLSWFCGNQLPAYKCFQITWFVSWTPCPDCVAKLAEFLSEHPNVTLTISAARLYYYWERDYRRALCRLSQ
    AGARVTIMDYEEFAYCWENFVYNEGQQFMPWYKFDENYAFLHRTLKEILRYLMDPDTFTFNFNNDPLVLR
    RRQTYLCYEVERLDNGTWVLMDQHMGFLCNEAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFIS
    WSPCFSWGCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFEYCWDTFVY
    RQGCPFQPWDGLEEHSQALSGRLRAILQNQGN
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
2. C19orf66
  • Gene Name : C19orf66
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 55337
  • NCBI Protein GI : 154350198
  • Genbank Accession : AC020931
  • Protein Accession : NP_060851
  • Taxonomy ID : 9606
  • Chromosome No : 19
  • Gene Starting Position : 10196805
  • Gene Ending Position : 10203927
  • Gene Strand (Orientation) : +
  • Protein Name : chromosome 19 open reading frame 66
  • Protein pI : 7.28
  • Protein Weight : 31422.73
  • Protein Length : 291
  • Protein Note : Also known as FLJ11286; FLJ40743
  • DNA Sequence : Show Sequence
    >gi|224589810:10196805-10203927 Homo sapiens chromosome 19, GRCh37.p5 Primary Assembly
    CGACTGCAGAGGCCGCGCCGCCTCCCCCGTCCGAGGTCTGCGCGCTCCGCCGCAGGGTGCAGACCCGGGG
    CGCCCGCCTGGGTTTGGGGCGCAAGAGCAGAGGCGGAGCCAGGGCGGAGCCAGCGCGCCGGGTCCCCCCT
    GAATCGAAAGCGAAACAGGGGCCGGGGAGGAAGGGCGGAGCCGGCCCCGAGGCACCGCCCCCTGCCCTGC
    GCGGCTGCTGGACCGACGGGCGCACCCAGGTAGGGGGGCGGCTGAGCCGCGCAGTGCGGACCCTCGCGGG
    GAACTGCGCCGCCGCCACCATGTCTCAGGAAGGTGTGGAGGTAAGCGATGGCTACGGCTGGGCCGGGGTC
    AGCCGCGACAGACCCCGAGGAGCGGCCGGGAGGCGCGGAGGGGGCTTCGCAGTTCCTGGGGACCCCCATC
    CTAGAACCCCAGATCCTTACCCCTGCCTGGCGCTCGCCCCCCTTGAAAAGGAAAGTGACTCCCACGTTTC
    CCCAGAGCGAAATGAGGCCTCCCCGAGGAAAAGCGGGGGCTGCAGGGTCAAAGGTCAGAGGCCAGAGATA
    ACCTGGCCGCCCCCCCACACCTTAGGCTGGGACGCTCGCCCCAGTCCGCGCCTTCCCCCAACGCCCTGTG
    GGGACTTTGGCTTTTTCCAGGAAATGCCGTAAAGGGATGAAAGGCGGGGGGGGGGCGGCGGAGGCCAAAA
    CCAAGGGTCAAGTTCGGGCCGGGAGAACGGTGCCTAGAGATGGGGGAGGGATGATCCCGTTTCCCCTTCC
    CCCACCGGAACCCCCCTGTCTCCATCCCAGCTGGAGAAGAGCGTCCGGCGCCTCCGGGAGAAGTTTCATG
    GGAAGGTATCCTCCAAGAAGGCGGGGGCTCTGATGAGGAAATTCGGCAGCGACCACACGGGAGTGGGGCG
    CTCCATCGTGTACGGTGAGGCTGCAGGGGTCCCGGGCCTGGTGCGGGGACCGCGCGTGGGAGCGCCGAGG
    GGGCGTGGTCTAGGGAGAGGCGTTGAGATCACCTCCCCCGGAGGTCCCAGCCTTGGGGAGGCTCTGCGTC
    GCGGCTCTGGGTGGCCTGGAACTCCCACAGACCCTTCAAGGGCCCTTCCCTTATATGCACTCTCCCCCGC
    AGGGGTAAAGCAAAAAGATGGCCAAGAACTAAGTAACGATCTGGATGCCCAGGTAACCTATCCCCTCCCC
    TCGCAAAGGACCGGGTCACGGGAGGGAGAGCAAGAGGGGGGACCCGACCCGTTCATGGTGACTGACCCCC
    CTCTGAAACAGGAGGGACCAGGCATTGTCGGTCCATAACTCATCACCCAATCTCACTTTTTCACCTCTCC
    ATCATTTGTCCGGCCAGGGAGCCTGCAAACAGTAGGCGCTCATTATGTGCTGATTGTATTCAATGGGGGA
    GAGAGCCATGAAACTAAGCAATGAAAGGAGCCAGGATTATTGCCATCATAGCGGGGGCGCAGAGGTCAGA
    GAAGGCTTACTGGAGGAGGAGGTACCAAGTCTTAAAGGATGGGGAGGAGTGAGCCCTGGGAGGGGAAAGG
    GAAAGAAAGGCAGGGGTCTTGGGGTGGGGGAGGGAATGGCATAAGCAAAGGCTGGGAGGTGGGGAAGGGT
    TGTTAGTGGCAGTCTCTAGAAAGGCTAGGATGAGCAGAGGATTGGGGAGGAGAGAGAGGCAGGGGAATGG
    AACCCTTGTGTTGAATGAATGAATGAATGAATGAATGAATGAATGAATGAATGGCTTTGAAGCACAGGTG
    CATCATGGCTACTTTGCTTCTTTGACAGCTTTGGTGATGGGAACGCTGTGGATTTAGCCTGCCTGAAAGG
    CTTAGTGTAGGGCTGTTAAAGAGTATAATAGGCCAGGCGCGGTGGCTCACGCCTGCAATCCCAACATTTT
    GGGAGGCCGAGGCGGGCGGATCACCTGAGGTCGGGAGTTTGAGACCAGCCTGACCAACATGGAGAAACCC
    GTCTCTACTAAAAAATACAAAATTAGCTGGGCATGGTGGCGCATGCCTGTAATCCCAGCTACTCGGGAGG
    CTGAGGCAGGAGAATCACTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCTGAGATCACACCATTGCACTC
    CAGCCTGGGCAACAAGAGCGAAACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAATGCCGGGCGCGGTGGC
    TCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCGGATCATGAGGTCAGGAGATCGAGACCAT
    CCTGGCTAACATGGTGAAACCCCATCTATACTAAAAATACGAAAAAAAAAATTAGCCAGTTGTGCTGGCA
    GGCCCCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTG
    CAGTGAGCCGAGATCGCACCACTGCACTCCAGCCTGGGCAACAGAGCGATTCTCCGTCTCAAACAAACAA
    AAACAAACAAAACCAGAGTATAACAAAACATGTATCATAGTCTACATTTATACATATAAAAGTATGATAA
    AAATGATAGCAGAGGGGCCAGGTGTGGTGGCTCACGCCTGTAATTCCAGCACTTTGGGAGGTTGAGGCGG
    GCAGATCACTTGAGGTCAGGAGTTCGAGATTAGCCTGGCCAACACGGTGAAACCCCTTCTCTATTAAAAA
    TACAAAAAATTAGCTGGGCGTGGCAGTGCATGCTTGTAATCCCAGCTACTTGGCAGGCTAAGGCAGAAGA
    ATCACTTGAACCTGGGAGGCAGAGGTTGCAGTCAGCCAAGATTGTGCCATTGCACTCTAGCCTGGGAGAC
    AAGAGCGAAACTCCGTCTCAAAAATATAATAATAATAATAATAATAATAATAATAATAATAATATTAGCA
    GAGAGTAGCATTCATTGAGTACTTCCCACATGGCAAGATTCCAGGAATTTATTTATTTCATCACTGCAGT
    GACCCCAGGAGACAGGTCCTATTATTTATCCCCATTTCACAGACAAAGAAACTGAGGCCGAAGTCACTTG
    CCAGCAAGCAGGGGCAGCTGCGATTTGAACCCTGGCCACCTGGAGTCAGAGCCTGGGCTCTAACATGCCT
    GTCAGTGGGAACCCAGGGGGCAGGGAGTGGCTTGCCCAAGTCCCCACAGCTGGAAGTGATACAGCTGGGA
    TTTGAACCCACATCTCTCTCCCACTTAGCCGAGCACAACGTGGCCTCAAAGGGGCGATATGTCACAACAT
    CAACAAGCATGTGGCATTTGCAGGCAGGAAGTGAGTATGGGGGTCAGGCTAGCCTCACTCAGGCAAGCCC
    TCCCCTAAGCCTCAGTTTCCCCATCTGAGCAATAAGGTAACAGGTGTGCCCACCTCTCAGGGCTGCTATG
    AGGACAACTGAGGCCACACACCTGGCTCTCAATAAGTGAGTGGTGCTCTGTGAGCTTCTGGCGAGTTAGG
    GGCACCCTACTCATTGCGGCCGGGGGCCTCCCCCAGCAAACGGGTGGAAAGAGGCCTGAGCCCCATCCCC
    AGTTTCTGCCCCTGCTATCTCCACCCAGGATCCACCAGAAGATATGAAGCAGGACCGGGACATTCAGGTG
    AGTTGGTGGCTAGGGCTTGGGATGGGGGAGTTGGGAGGGGGACATCTGCAGGCACAGAAGGATGTCCATT
    AGGGCAGGAGGGGAGATATTCACTGGGGCAGGAAGAGGACTCGTCTGTAGGGAGAGGAGGGCTGATGCTT
    ATGTTGGTGCAGAAGGGGTGACACCCCCCCACCTCATCCCCACCTGCCCCATTCCACAGGCAGTGGCGAC
    CTCCCTCCTGCCACTGACAGAAGCCAACCTACGCATGTTTCAACGTGCCCAGGACGACCTTATCCCTGCT
    GTGGACCGGCAGTTTGCCTGCTCCTCCTGCGACCACGTCTGGTGGCGCCGCGTGCCCCAGCGGAAGGAGG
    TGATGACCTAAAACTTAACCTCGACTTTGACTGTCCCGAACTCCACTCCTGACTCCTATCCTCAGTGACT
    GTACCTCTGACCTAAGGATTTCAATCACTGAGAGTCCAATCCCTGACCTCCAAACTCAGAGCTCAGCCTT
    GATCTCTGATCCCAACCCCTGACCCAGGACTGGACTTCTGACCTTAAATGTGAGAAGCCCAATACCTCCT
    TACCCCAGAACCTGACCCTTGACCCACAATTGAACAGCTACTACAGAAATCAAACCTGGCCAGGCGCAGT
    GGCTCATGGCTGCAATCTCAGTGTTTTGGGAGGCTAAGTAGGGAGGATCGCTTGAATTTAGGAGTTTGAG
    ACCAGCCTGGGTGACAGGGTGAGACCCTGTCTCCAAAAAAAAATGTGTTTTGAAGACAGGGGGTCTCGCC
    CTGTCACCCAGGCTGGAGTGCAGTGGCGGGATCATAGCTCACTGCAGCCTCCACCTCCTGGCCTCAGGTG
    ATCCTCCCACCTCAACCTCCCAAGCAGCTGGGACTACAGGCCTGCGCCACCACACTTGGCTAATTTTTGT
    ATTTTGTGTGTGTGTGTGGAGATGGGGTTTTGCCATGTTGCCCAAGCTGGTCTCAAACTCCTGGGCTCAA
    GCAATCTACCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGTGCCTGGCAAAAAAAA
    ATTTTTTTAATTAGCTGGGAGTGGTGGTGTGTGCCTGTGGTCCCAACTACTCAGGAGGCTGAGGTGGGAG
    GATCGTTTGAGCCTGGGAGGTCGAGGCTGCAGTAAGCCATGATCACAACACTGCATTCCAGCCTGGGTGA
    CAGAGCAAGACTCTGTCTCTAAAAAAAAAAAGAAAGAAAGAAAAGAAACCAAATCTGACCTCAAAAACAC
    AGCCCCAAATCTGGCTTCTGAACCTCCTGACTAGTAGTTCACTTCTGGTCAATGCTTAACTAGTTCTTGC
    AAAAGAGGCAGGAAGCCAAGATGTGTAGTGTTTGCCAATTTCTGTGGTGTAAATATTCCCACCATGGTCA
    ATATCAGGCTACCCACGTGAAGTCACTGATTGAGTTGGGTTGCTCAAGCTGAGGCCAGCCGCTGCAGCAC
    ACTGCTAGCCCTGACCCCAACCTCAGACACCTCTGACAATCCTTGGTCCCCTCTCTGTACCTTCCTGCCC
    ACCACCACCAGGTATCCCGGTGCCGGAAATGCCGGAAGCGCTACGAGCCAGTGCCAGCTGACAAGATGTG
    GGGCCTGGCTGAGTTCCACTGCCCGAAGTGTCGGCACAACTTCCGGTGAGGGCGCTGACCCCCAGCTCCC
    CCTCAGCCCTGCCCTACCCCAGCCCTGCCCCTCCCTGCCCTGGCCCCACCCTGGCCCAGCCTCGCCCTCG
    GACCCTCACAGCCCTGCCCGCCCCCAGGGGCTGGGCACAGATGGGGTCCCCGTCCCCCTGCTACGGGTGC
    GGCTTCCCCGTGTATCCAACACGGATCCTCCCCCCGCGCTGGGACCGGGACCCGGATCGCCGCAGCACCC
    ACACTCACTCCTGCTCAGCTGCCGACTGCTACAACCGGCGAGGTGAGGCTCTTCTCCCCCAACAGCCTGG
    ACAGTCTTTGTCCCCTTCTGTGCCTTTAAGTCCCCAAATCTCCACTCAGTCCACTTTGTCTCCCACAGCA
    GCAGCCACTGCTTCCACATGGCCTCCATGACCCCCCAGTCTCCGTGGTCTTGCCCAGGAGGCTCTGAGGT
    TTCACCCCAGTGGCCCGTGCCTGAGGGTCCCCATGGCCTCTGCCCCCATGGTCTCTGGGTTTGGGGCCCC
    TGTTTTGTCCCCTGTAATCTCAGGTGACCCCCATGTCCCCAGGTCTCCTGTATCTTCAACACCCCTGAAT
    GTCCAGTTGTGCTGGTCCCCGTATCTGGTGGTCTCAGCTCCTCCCTAGAGCCCCGCGTGGCCTAAGTCCC
    CTCCTCCCCAGACTCTCATGGGACCTCCAGCCCCATGTCTGCAGCACCTCTCCCTCCCTAGAGCCCCACG
    TGCCTGGGACATCCTGTGCTCACCCCAAGAGCCGGAAGCAGAACCACCTGCCCAAAGTGCTCCACCCCAG
    CAACCCTCACATTAGCAGTGGCTCCACTGTGGCCACCTGCTTGAGCCAGGGTGGCCTCCTGGAAGACCTG
    GACAACCTCATCCTGGAGGACCTGAAGGAGGAGGAGGAGGAAGAGGAGGAGGTGGAGGACGAGGAGGGCG
    GGCCCAGGGAGTGACCCCTGCCAGGTGCAGATACAAACCAGACACGGTCTGTGGCTACTTTGTGTTATTA
    TAAGATATGAGCTCAAACCGAGATATGAATGACCTTGGGGAGCCATCTGAGGCCAAGATATTGACGGGGG
    GGATTCCTGGGTCCCATTTTCAGCGCCCAGGGTCACAGATCCACAGTGGGAAGTTCTGTGGGACACATTG
    GCACTGAGCCACAAAGAAGGTGTGGCCAGAACAACTTGGGCTCCTGCTGACCAATGTCCTCTAGGGCCTA
    GGGGACAGAGGAACACAGAGTCACAGCTTCAGGGGCCGAATGAGCATGGCGGCCTTCCTGAGAGAATATG
    CCCCACCACGAAACTCAGCCCAGTAGACACCATCCTGGTAGCGGCTTCGGTAGTGGCCGCCGTGGTGCCA
    CACACCGTTGAGGTTGGAGTGGGCACAGGCATGGTACCACCAGCCTCCCCGCTGGTACAGGGCACAGTTA
    CCTGAGGGGAGAGAGAGAGTCCATGTCCTCTCACCAGAATAAAAGCCTCTACCTGCACCTCACAGTGCAA
    GGCTTTTGCCAGGCATCCCCTGGCCCCTCCCATTCTTATTGAATACAAGCCCTGATCTTCCATCTCCTCA
    GCAAAAAAATAGGAGCCCTGGCCCCCCAACTTTCTTCAGAGTAATAGCCTTAATTCCTTCCCTATCTCCT
    TACCAAAGTACAAGTCACATCTTTCCCACCTTTTCTGCAAACTAGGAGTCTACCGTTCATTCCTTTATCA
    AAGAAAAGTATCTACTTCCTTTCTAGAATAAGAGTACTAGCTCTCACCCTCTGCCCTTTACTTGAACAGG
    AGTCTTGATTCTTTTTTTGCCTCATCAGAGAAGGAATCTGGACTCCCCATCCCCCCACCAGGATAAAAGT
    CCTGACCTTTGTTCTCTTGACGGAATAAAAGCTTGCTTATCCTTATACTTACC
  • Protein Sequence : Show Sequence
    >gi|154350198|ref|NP_060851.2| UPF0515 protein C19orf66 [Homo sapiens]
    MSQEGVELEKSVRRLREKFHGKVSSKKAGALMRKFGSDHTGVGRSIVYGVKQKDGQELSNDLDAQDPPED
    MKQDRDIQAVATSLLPLTEANLRMFQRAQDDLIPAVDRQFACSSCDHVWWRRVPQRKEVSRCRKCRKRYE
    PVPADKMWGLAEFHCPKCRHNFRGWAQMGSPSPCYGCGFPVYPTRILPPRWDRDPDRRSTHTHSCSAADC
    YNRREPHVPGTSCAHPKSRKQNHLPKVLHPSNPHISSGSTVATCLSQGGLLEDLDNLILEDLKEEEEEEE
    EVEDEEGGPRE
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
3. CD70
  • Gene Name : CD70
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 970
  • NCBI Protein GI : 4507605
  • Genbank Accession : AC008760
  • Protein Accession : NP_001243
  • Taxonomy ID : 9606
  • Chromosome No : 19
  • Gene Starting Position : 6346586
  • Gene Ending Position : 6351901
  • Gene Strand (Orientation) : -
  • Protein Name : CD70 molecule
  • Protein pI : 8.6
  • Protein Weight : 19828.21
  • Protein Length : 193
  • Protein Note : Also known as CD27L; CD27LG; TNFSF7
  • DNA Sequence : Show Sequence
    >gi|157718668:6346586-6351901 Homo sapiens chromosome 19, alternate assembly HuRef, whole genome shotgun sequence
    CTGATTTTTGTATTTTTTAATAGGAAAATGAAAGAAAAAGCTCAATGCCTTCTCTTGTCCTGCCACCACT
    ACCCCCCACCACACTCCCACCCCAACCCCGGGTGGCCCCTGTGTGTACACTTTTTCTCTTGAACTTAAAT
    AAAATAAAATAAAATTTAAAAAACCCTAATCAGCAGCAGTGGTCAGGGGCGCACCCACTGCACTCCAAAG
    AAGGTCTCATCAGTGTTTCGGGAAGGCAAAAGTGTCCCAGTGAGGTTGGTGCAGAGTGTGTCCCCTCGGG
    CCAGGGGCGTCAGGCGCTGGGAGGCAATGGTACAACCTTGGTGGAAGCTGAGACGCAGCAGGCTGATGCT
    ACGGGAGGCGGGAGAGCAGATTCCCACGGCCAGGGTGGTGGGGTGGTGCCTGGAGGCCGTCGTGGAGGAG
    CAGATGGCCAGCGTCACCTGGATGTGTACCATGTAGATGCCATCACGATGGATACGTAGCTGCCCCTTGT
    CCAGCTCTGGTCCATGCAGGAAGGAGCGGCCCAGTGCTGGGCCCCCCTGCCAGTATAGCCTGGGGTCCTG
    CTGAGGTCCTGGGGGCACAGGGTTAGAGGGATGAGAGGATGGAGGTTTAGGGAAACTGAGGCACAGGGGG
    TCATAGAGAAAGTCAGATATAGAGATCGAGAGTTAGAGATCAAGGAATAGGTTGGACGTGGTGGCTCATG
    CCTGAAATCCTAACAGTTTAGGAGGCCAAGGCGGGTGGATCACCTGAGGTCAGCAGTTCAAGACCAGCCT
    GGACAACAGGGCGAGACCCCATCTCTACAAAAAATAAAAAGTCAGCCAGGTGTGGTGGTGCACGCCTCTG
    GTCCTGGCTACTCTTGGGAGGCTGAGGCTGGAGGATCGCTTGAGCCTGGGAGGCTGAGACTGCAGTGAGC
    TATGATCACACCACTGCACTCCAGCCTTGGTCACAGAAACCCTGTCTCAAAAAAAAGGGAGGGGGATAGA
    AGGTTAGAGATCTAAAGGGAAGGAGAGAGGGGTAAGGGGAAAGGGGTAGAGACAGGGAGAGAGAGACACC
    AGGAGAGAGAGAGACAAAAAAAAAAAAAAAAAAAAAACAGAAACAGACAAGGGTAAAGATAGAGGGGGAG
    GGAGAGATATATAGAGAGAGAATGCAGAGAGAGAGCAAGAGAGAGAGTGTATGTGTGAGAGAGGACGAGA
    GTGCACGAGAGAGTGCGACAGAGAGCATGAGAGAGAGAGCATGAGAGAGCGAGAGAGTGTGTGAGAGGAC
    GAGAGAGCTTGAGGGAGAGTGCACGAGAGAGTGCGAGAGAGCACGAGAGAGCAGGAGAGAGTGCAAGAGA
    CAGCATGAGAGAGAGAGAGATCATGAGGGAGAGAGAGCATGAGACAGCGAGAGAGTGTGAGAGAGAGGAC
    GAGAGAGCTTGAGGGAGAGTGCACGAGAGAGTGAGAGAGAGCACGAGAGAGCAGGAGAGAGTACGAGAGA
    CAGCATGAGAGAGAGAGCAAGAGAGAGAGTGTGTGTGTGAGAGAGGACGAGAGAGCTTGAGAGAGAGTGC
    ACGAGACAGAGTGTGAGAGTGAGCTCGAGAGAGTGCATGGGAGAGTGTGTGTGTGAGAGAGGATGAGAGC
    TACAGAGCTCGAGAGTGCACGGGAGTGAGCGAGAGAGTGCCTGAGAGAGTGAGACAGACAGCACGAGAGA
    GACAGCGTGCGCACGAGACAGCGCGCAAGAGAGTGCACGAGAGAATTCCCCAAGGGACGCCTGCTCGGGT
    CACACCCTGAACGCCAATAAAAGCTTTGGCTCCCAGGACCCTTTTTTTTTTCTTGCTTTCCACCCGCTGG
    TTGAGTGTGTGTCTCAGAGAGCTCCCCTACCTTCCCGTTGGCCCTCTTCTCTCTAGGATCTTTATTTTCT
    TTAGAGACAGGGTCTGGTCCTGTCACCCAGGCTGGAGTGCAGTGGTGCAATCATAGCTCACTGCAGCCTC
    AGCCTCTGGGGCTCAAGCTATCTCCCACCTCAGCCTTCCCGAGTCACTGGGAGGTGGCATGCGCCACCAT
    GACCAGCTAATTTTCAAAATTTTGTTCTAGAGATGGTGGTCTTGCTATGTTTCCCAAGAAACATAGTCTA
    GAACTCCTGGGCTCAAGCGATCCTCCCACCTCAGCCTCCCAAAGCGCTGGGATCACAGCCGGGAGCCAAG
    GTCCTCGGCTATATTTCACGTGTTTCATCTCAGCGTCTCCTGTCTCCGCAACTTATCTGACTGACACACC
    TGAGCCTCACTCTCCTCCTCACTCAAGGCTATAGTGGAGACTGGCTATCTTGTTAGGAAGGAACTGACAC
    GGGGTCAAACAGGAGCCCCAAGGGTGTTTACCAGGCTTTGCAAATTTCCTGTAAGAGTGACACCTGGTCC
    CCTGTCAGACACTTAGGCATGATATCATCCACCAGAAGAAAGAACTGTCCTGTGAAAGACACTGTCAACA
    CCTGTGGCCACCACCCCTGGTGCCCCATCGATGTGGGGCTGGACAGCCACTCTCCAGAAAGGGGCCTCAA
    GACCAAATTACAGGAAAAATATGGCAAAGGCCGGGGAGGGGGGGAAGATATATCAGGGGTCAGAGACCCA
    AGGAGAAGAGAGAGCAACAAAGGTCCCTTCCATTACAAAATGGAAGGAACAAAACACGCAAGCGTGCAGT
    AATTTTAGTCTCTGGGGATGAACCGTGGAAAAACAGAAGAGGAAGAAGCGTTCGAGAGAGGCTGGGAGGA
    GGTAGAGACTTCCACCTAGAGCAGAACTGTTCATTTCTTCTCCCTCAAGGCTTCTTGCCAATGCCACCCA
    CTCAGATACCACCCCCCCACCCGACCGCCGCGACTCCACTTTAAATGAAAATCCCCAACCCTGGCATGCC
    CGGATTCCCCACCCACCTCCTTGCCTGATTTTTTCTCTAAAACCTGCTCTTGGCCTGTTTTACTCTACGT
    GGCATTGCTCTGTTTTTCACATGTTCGTTTTTATTTTTTTGGTCATCTCCTCCTCGGTAGGAGTGATAGT
    AGAAGGGAAGCTCACCAGGCAGGGATTTCTGTCTGTTTTGTGCGCGACTGCAGTACCGGAACAGTGCCTG
    CCCACTGCAGATGTATAATTAGTGCTTGTAGCATGAATGGAGGAAGGGTAGCCTTTCCTGTGAGCGTTTT
    CGTTTACTAACAAAACCCCTTTGATTCTTCCAAGGGAGGAGATCGAATGTGAAGGACTGAGAGGGAAAGG
    GAGAGAAAGGTAGTGGATGCCCAGAAACACAAACCTGCTCGCTCGCTCTCTCCTCTCTCTCTCTCTCGTT
    CTCTCCCTTTTCCTTTTTCTCTTGGGAATGATCTCTTTCTCCCCCGCCCTGTTTTTCTTCTTCCCCCTCT
    CTGTCTCTGTCTCTTCCTCTCTGTTATTCTCCTTCTCTTTTCCTCTCTCTGGCTTTCTTTCTTTTCTTTC
    TCTTATTTCTTTTTTTCTCTTTCTTCTTTCTTTTTCCTTTCTCTCTCTCTCACCTCCCTCCCTCTCTCCT
    TCCTTCCTTCTTCTCTTTCTTTTCCTTCCTTCCTTTCTTTCTTTTTCTTTTCCTTTCTTCTTTTTATTTA
    TTTATTTGACAGAATCTCCCTCTGTCACCCAGGCTGGAGAGCAGTGCCATTATCTTGGCTCACTGCAACT
    TCTGCCTCCCAGGTTCAGGCGATTCTCCTGCCTCGGCCTCCTGAGTAGCTGGGATTACAGGTGCCTGCCA
    CCACACCCGGCTAATTTTTGTAATTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAA
    CTCCTGGGCTCAAGTGATCCACCCGCCGCGCCCTCCGAAAGCGCTGGGATTACAGGCTCCAGCAACCACG
    CCAGGTCGCCTCTCTCGTTTTTATTCTCTCTTCCTCTATCTGTCTTTTTTCTCTTTCTCCGTCTCTCTCC
    CTCTCTCTCGTTCTGTCTCCCCCGCCTGTCTCCCTCCCTCCCTCTTCCACTCTCCCTGCGTCTCTCCCTG
    TTTCTTTCTTTCCCCTTCTTCTCCTGTCTCCGTCTGTCCCCTCTGTCCCTCTCTCTGTTCTTTTTTCTGT
    CTCTCCCTCCCTTTCTCTGGGCCTCTCCCTCCCTCTCTGTCTTCTGTCTCCCCCTCTCCGTTTCCCTCCC
    TATCTCTCCCTCCCTCTCTCCCTCCGTCTCTGTCTGTGTCTCTTTCTCTCTGTCTCTCCCCACTTGTCTT
    TCTACCTCTCCTTCCTTCTCTCTCTGTGCCTCTTCTTCTCCCCGTCCCTCCACGTCCCCCGTGTTACCTG
    TGTGATTCAGCTGCAGCTCAGCTACGTCCCACTGGAAGAAAAGACCAGAAAACAGGGCACGGACGTAAGC
    AGAGAGGTTCTATGTGTCCCCTGTGCCAGGAGCTCTCTTTTCTCTGTCCATCCTCCTTTCCACCTCTCAT
    CCCACGGCGCGCACTGGTGATTTTATTTCATTTTATTTTTTTTTACTCTTAAGACTTCTTAAAGAGGAAG
    CAGGTCTGAACCAGCGGGGAACGTGAAGTCGAGACCTTCAAAAGAACTTCTTCGTTTCCCGAATCGTTTT
    CCTGGATGTCACTGGGCGCGAGAGCACCGCGCGCAGCGGACTCTCTACGCTGCAAAGGCGCCCGGCGCGG
    TCCCCAAAGGGACCCCTCCCCCACCCGGAGCGAGTGGCTGGGGCCATCCCGTCCTAGGAGGCCCCAGGAA
    GCCCCCACGCCCAGCCTTGTGAAGCCCCCAGCAGCCTCTGAGTCAGCCTGCCGGGGGAACACCAGAGCCG
    AGTCATCTTCCATTTCCATGTGTGCCTACCTTTCCCATCCCGGGATCTCCCTGTACCTCCTGGCAGGGCT
    GCCTGTCTCGTAGCCCCTACACCGGGCAGCCTGGTCCCCGCCTCGCCTCCCTGTTCTGGTCTCTGTCTCT
    GCCCTACCAGATCTTCCTCTCTGGCCTCTTTGTACCCATCTCATTCTGTCTTTTCGGTCACGCGCCTCTC
    TATGTTTTCTTCCCAACTTTTCCATCTCAACTCACCCCAAGTGACTCGAGCGGCAGCTGCTGCTGAGCCT
    GTGCGAAGCGCTGGATGCACACCACGAGGCAGATCACCAAGCCCGCGACCAATGGGACCAAAGCAGCCCG
    CAGGACGCACCCATAGGGCCTGCGCCGCACCGAGCAGCCCGAACCCTCCTCCGGCATCGCCGCGGCGATC
    ACCTCCGCTAGCGCAGGAGGGGCGATGGGGGCGCGGAGCGCTGCCGAGAAGGAAGGAAGGAAACTGCAGC
    CCCCTCCCGGGGCTCCTGGGCGTCTACTTGCTTCAACCTGTCAGGGGACCAGCCTGCCCCTCTCTG
  • Protein Sequence : Show Sequence
    >gi|4507605|ref|NP_001243.1| CD70 antigen [Homo sapiens]
    MPEEGSGCSVRRRPYGCVLRAALVPLVAGLVICLVVCIQRFAQAQQQLPLESLGWDVAELQLNHTGPQQD
    PRLYWQGGPALGRSFLHGPELDKGQLRIHRDGIYMVHIQVTLAICSSTTASRHHPTTLAVGICSPASRSI
    SLLRLSFHQGCTIASQRLTPLARGDTLCTNLTGTLLPSRNTDETFFGVQWVRP
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
4. Cxcl10
  • Gene Name : Cxcl10
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 15945
  • NCBI Protein GI : 10946576
  • Genbank Accession : AC109603
  • Protein Accession : NP_067249
  • Taxonomy ID : 10090
  • Chromosome No : 5
  • Gene Starting Position : 92346637
  • Gene Ending Position : 92348888
  • Gene Strand (Orientation) : -
  • Protein Name : chemokine (C-X-C motif) ligand 10
  • Protein pI : 10.57
  • Protein Weight : 10391.5
  • Protein Length : 98
  • Protein Note : Also known as C7; IP10; CRG-2; INP10; IP-10; Ifi10; mob-1; Scyb10; gIP-10
  • DNA Sequence : Show Sequence
    >gi|372099105:92346637-92348888 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
    ATTTCATGTCAGTTATTATTACTTTATTACATTTTGGGTCTCTTACTACTTTCAATACAGTACATTGTCT
    TTTGAATGTTACATAATACAAAAGGTATTGGACGGTTTTAAAAATAAACTTTAACTACCCATTGATACAT
    ACTTGATGACACAAGTTCTTCCATATACAATGCAAAGCATACAAAAAATACATTAGGAATTCTACTTTGT
    ACAGTCGTTCATTAAATAGTATTTACACATACATTTTCAGGTTCCTCTGAGTATCTTGATAACCCCTTGG
    GAAGATGGTGGTTAAGTTCGTCCTTACAAACTTAAATTTGTAGTCTTACATCTGAAATAAAAGAGCTCAG
    GTAAACTTAGAACTGACGAGCCTGAGCTAGGGAGGACAAGGAGGGTGTGGGGAGCAGCCTGGGCATGGCA
    CATGGTGAAGGGCGTCGCACCTCCACATAGCTTACAGTACAGAGCTAGGACAGCCATCCCAGCCACTTGA
    GCGAGGACTCAGACCAGCCCTTAAAGAATTCTTGCTTCGGCAGTTACTTTTGTCTCAGGACCATGGCTTG
    ACCATCATCCTGCAGGAGGAGTAGCAGCTGATGTGACCACGGCTGGTCACCTTTCAGAAGACCAAGGGCA
    ATTAGGACTAGCCATCCACTGGGTAAAGGGGAGTGATGGAGAGAGGCTCTCTGCTGTCCATCCATCGCAG
    CACCGGGGTGTGTGCGTGGCTTCACTCCAGTTAAGGAGCCCTTTTAGACCTGTAGGAAGAGGAAACTGTA
    TTGAGCGGAATAGACTCTGCTTTCACTTTGGGAACCCAAAGACCCTGGGGCAAAAGGCCTGGTGCATTTT
    CCTCTGCTACACAATCTCCATGGGTCACGGGTGGGTGGGGGTTGAGGGGACAGAATGGGTTTGTGTGTTT
    TTTGTTTTTTTTTTTTTTAAGTGAGGAAGTGGGGGGGGGGTGGGCATTTGGCAGCTTTACCCGTGACAGG
    TATAATAACTGCTCCACTTCTAGACACTAGGAATGTATGACCTAAGAAGGCACCTCAGTGTCTGTGTAAA
    TGGAGAGACAAAGGCACATGCCGGTGCCATGAGTTTAGGGCAGATGCCCCACAGCAAAGCCCAGGGCAGG
    CGCTGCTCCCCAGACCACCATTTCCTGAAAAAGCAACATCAAACCTACCTTTTTTGGCTAAACGCTTTCA
    TTAAATTCTTGATGGTCTTAGATTCCGGATTCAGACATCTCTGCTCATCATTCTTTTTCATCGTGGCACT
    ATAGAAATAAGAGTGAGATACTCAGATTAACGTCAGGCCAACAGAGCTGATGGCCCTGCAGGTCCATGTC
    AGAGTTCAGGCACTGTGCCTTAGTTAGAGTCACAGACCCGTCCCTATCTTTCCTGGTTTCCCTCACTTAT
    TTACTGTGTGGTTACGGGAGGGACGTTTATCGGCAGGTGGGCTTGTACTCACATGATCTCAACACGTGGG
    CAGGATAGGCTCGCAGGGATGATTTCAAGCTTCCCTATGGCCCTCATTCTCACTGGCCCGTCATCGATAT
    GGATGCAGTTGCAGCGGACCGTCCTTGCGAGAGGGATCCCTGCAGGAAGAGAGGGGGAGAGCCAGGAAGG
    TCAGCACACGGTTTCATTTTAGGCACTTAAAATGGATGAGGTAGTGTTTAGCAGACCCTGTCTGATCTGT
    GGAGAGACGCATCCCATCGTATCATAGGGCTGAGTGTTTGAATATCTTGGTCATGGTAAAGAGAGATCTG
    CAACTTGTCAGTTACGAAATCCTGCATTTATGCACTGCTCACAGTATTGGTTAGGCTCAGGCAAGGAGGC
    ATCCGTGCCTTACCCCATCCCACTCTTTCGCCCTTTCTCCTGACTCTCTCTTCCCTCCTTCTTCCTCCTC
    CCTCCTCCTTTTCTCCCTTTCTTCCCTTCTTCGTTCCTTTGCTTTTATTCTATTTTTCTCTGACTTCAAC
    CAGCCTATGATAAACTCTGCAAGCTGAAGGGATTTCTTCCTGCTTCCCTCTTCTTTATAGGTACACACAT
    TAAAGCTTAAATACATGCTGTGTTTAGAACTGAGCTTAATCGTCTGACTTCTGACTTCGTTAATTAAATG
    GCCTTGGTGTCCCTTACCTTGAGTCCCACTCAGACCCAGCAGGATGAGGCAGAAAATGACGGCAGCACTT
    GGGTTCATGGTGCTGATGGGGAGGCTTCCGGAAGGTTGGCTCGGGATGTCTCTCAGCGGTGGATGAAGCG
    CTTCTCGAGTGC
  • Protein Sequence : Show Sequence
    >gi|10946576|ref|NP_067249.1| C-X-C motif chemokine 10 precursor [Mus musculus]
    MNPSAAVIFCLILLGLSGTQGIPLARTVRCNCIHIDDGPVRMRAIGKLEIIPASLSCPRVEIIATMKKND
    EQRCLNPESKTIKNLMKAFSQKRSKRAP
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): MVA/IL-15/5Mtb vaccine
5. Cxcl11
  • Gene Name : Cxcl11
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 56066
  • NCBI Protein GI : 9507071
  • Genbank Accession : AC122365
  • Protein Accession : NP_062367
  • Taxonomy ID : 10090
  • Chromosome No : 5
  • Gene Starting Position : 92359543
  • Gene Ending Position : 92363295
  • Gene Strand (Orientation) : -
  • Protein Name : transcript variant 2
  • Protein pI : 11.02
  • Protein Weight : 10670.03
  • Protein Length : 100
  • Protein Note : Also known as Ip9; H174; Itac; b-R1; Cxc11; I-tac; Scyb11; Scyb9b; betaR1
  • DNA Sequence : Show Sequence
    >gi|372099105:92359543-92363295 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
    CTTTACTTTAAGATAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGAAACTCCTGGTCTGTAGACCAGGTT
    GGCCTTGAACTCAAGAGATCCTCCTGCCTCTGCCTTTCAAGTGCTGAAATTAAGGTGTATACCACCACAC
    CTAGGTAATTTTTTTTATTGAAATTTTTAACAGTACCTTTTTATCATATTTTTTTCTCATCTTCAGCTCC
    TCCTCACTAAAGTATATTCCTTTCTATTTTAAAATTTTGTGTAGCCCTGACTGTTCTGAAAGACCAGTCT
    GGCCTGGACCTCACAGAGATCCGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCGTGTATGTTTCT
    AAAGAATTCTGACAAAAACTTGTGTAGCTCTAAAAAACAAAACAAAACAAAACAAAATCTAAAGGCATCT
    CAATGTCCAGAGAGCATGTAGTTGTTGATGATTTAATAAGTGTTATAATAGCTTACTAGAGAAACACAAT
    TCTTATTCTTTTCAAGGAGAGACAGAGCCAGCCATCCCTACCATTCATTCACAGTATGACCAAGGAAAAG
    AACAAAGTACATATTTATATATTTTTCTCAAAGGCAGACTCACAGGAAACAGACTGAAAGAGGTGCACAG
    ATTTTTTTTTCAACATTTTTCTTTGGTGAACAGAACCAATTTATTTTAAAAATCTATCCTTGTTATTGTC
    ACTGATAAAACCAGACGTACAGGAAATACACATCTTAACCCCTTAGAAGGCCTCAGGGGGAAGCCAGTGT
    GACTAGCTAGGAAACAAACAGTAGTGTTCTTACTAGTTTCTACAACGCAGAAATGAATCGTAGTCTTTTT
    AAATGAACAAACAAGACAAAATGTGGAAATGTCTTCCTAGATGTTCGTGTGCCTCGTGATATTTGGGGAA
    AGGAATGTTTATTTGCATGAACAATTCTTTGAGGAATGTTTTACTACACACAGAGATGCTTTTGAATCCA
    GCACTGGTCCGGATTGCAGTAACAATGCATCCACTGTAACCACAGAAGGTAGCGTGGAGTGTGCACGTGA
    CTTTTAGAGACAAATATTTTTCATAGCCAAGTAGAACAGTTTAGATGTATCGGACCTTCTAGAAAGTTCT
    GCAGCCTGGTAATACGTGGCTGCATGTTCCAAGACAGCAGAGGGTCAGGTTCCTGGCACAGAGTTCTTAT
    TGGAGGGCTCACAGTCAGACGTTCCCAGGATGTCACATGTTTTGACGCCTTAAAAAATTCTTTTTTTCTA
    TTGCCTGTAATGAGAAAATGAAGAGTTTTACTTACTGGGTTGTTTCGTTTCCTCAGTAATTAAGAGCTGA
    CCTGTAAAAACAACGCGGAGGGGTAAGGATGTGGCCAGGTTTAACCCAGGACCCCTTAACTGGGTGCTTC
    TGTTAGGATGGTAATGGGGTCTTCCGGCAGTCGCCTGTTCTCTGTGTTCCAGCACTCTCGTGGGTAAAGC
    ACTTTCCGACCTGGCCGACTGGCAACCTACCTGCATTATGAGGCGAGCTTGCTTGGATCTGGGGTCCAGG
    CACCTTTGTCGTTTATGAGCCTTCATAGTAACACTGGTAAGGGAGACACAAAAGAGTCTTAAACTTTGGT
    GGGAAAACATCAACCAAACAAAAGCACCCATACCATTGTCACCTCCAGCACATACATCACTTCAACTTTG
    TCGCAGCCGTTACTCGGGTAAATTACAGAAGCTTTCTCGATCTCTGCCATTTTGACGGCTTTCATCCCGG
    GGCCGATGCAAAGACAGCGCCCCTGTTTGAACATAAGGAAGCCTGGGACAGACCACAGTGTTGGTTAGTC
    AGGGCTGTGAAGTGGATGGCAGACGCATTTGTTAATCTCAACTGTGCAGACTGAAATTAAGTTTTTGAAT
    CTCTCTGCATTAGGCTAATTTGCTGTATCCTTGATGATTTCCTTCCTTAATTTTTGTTAATTATTTAATT
    TAGGTTGCAGACAGGACTCACTTGCTATTTGGACCAGGTTTGCCTCCAACTCACCACAAAGCCCAGGATG
    ACCTTGAACTTGAAGCACTCACTACAAAGCAGCCCCCTCCCCCCATCTTGGCATTATCCTACCTCACTCA
    GCTCACCCTCCTAAGTGCTGAAGTTATATTACAGTATGAGTATGCTATGAGACCGGCTCTTGATTACTTT
    AAATAACACCTACACAGTATACAGCAACCCAGAGAACAAAGTGGCTATTTATTTGGAATTATTTTATGAC
    TGGTGGAATGGGTATACAAGCGTCTTTCACAGTCGCTGGTGATCTGTTCTTTACTAAATGAGCAAATAGT
    TGATAATTTCTTTGATCTTTGTCTTCCCAGTTCAAATTCTAGATTAAGACTAAGTATTATGAATCATGTT
    GTCTCCCACAGCTGAAACTGTCTTTAAAGCTTCCAGATGCTCCCAGGGAAACCTCAAAGGTTCATTATTT
    CATAAGAGCAGCAAGAGTTGTAATAATGGCTTGGCTTGTCACATTAACTTCATATCTGGCTTAGCCCAGG
    TCTGCTGTGATAATCGTGTAAGAACCATCAGCTCACAGGAGAAACACTTCCAGGTTCAGTCATGGGACAT
    GTGAATAATGCTTCTATTTAGAAACAATATTCTTTGGGAAGAGCTTGTGGAGCATTGTCAGTATCCACAC
    TACCTTTAAGTCACTCTTGCCCTCAAGTGACATATTGATCAAGCACGTATGACATTTTTATGACCATTGC
    CCCAGAGAGAAATTTCCTTTTCTCTATTCTGAAAGTATTGGTCATAACAAATTAGCAGTCTCCTGGTTAA
    AACATTAATTTTTTCAGAATACCAGCATAATCCCACATAAGATTCTCTGAACAGGCATTGAGGACTGGAT
    AATTGTCAGAAAATGAAGAAATTGGGCAGGGGTGGCGCACGCCTTTAATCCCAGTACTAACAACTGTGGA
    GGAATACAATGGCATTGTTTACTGCCTAATATTTGTAAGTTGCTCTGACCATAATATCTGTGGTTTATTT
    GAAATACAAATCGCCAGGTGTGGTGGTGCACGCCTTTTATCCCAGCACTTGGGAGGCAGAGGCAGGCGGA
    TTTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCC
    TGTCTTGAAAAAAACCAAGAAAGAAAGAAAGAGAGAAAGAGAGAAAGAGAGAAAGAGAGGAAGAGAGGAA
    GAGAGGAAGAGAGAGAGAAAGAGAGAAAGAAAGAGAGAGAGAGAGAAAGAAAGAAAGAAAGAAAGAAAGA
    AAGAAAGAAAGAAAGAAAGGAAGGAAGGAAGGAAGGAAGAAGGAAAGAAAGAAAATGAATCCATAAACTG
    ATCATTTTCTTTAAGGATTTCCAACATTTGCTGCGCCATTGCTAGAACTTCTTTTTTTAATTATTTATTT
    TTTATTAGATATTTTCTTTATATACATTTCAAATGCTATCCCGAAAGTTCCCTATACCCTCCCCTTAGAA
    CTTCTAATATGTGAAATAAAGTAAATTTTTAAAACTGTATATTTTAGAGAAAAAGATATTTGAAAAGTAA
    CACAAGGAACAGACAGACTCAGAAGCTACGGGCACCAAATACCTTGAGCAGCTGTGGCCCAGATATTGCA
    GCCAGGGCTATGGCTGTGACCTTCCTGTTCATCTCAGCAGCCGCTGCAGCTCTCTGCCTGGGCTTTGGAG
    ATCTCTCTTTGCAGAGAGCAACGGCAGGCAGGAAGGCTGAACC
  • Protein Sequence : Show Sequence
    >gi|9507071|ref|NP_062367.1| C-X-C motif chemokine 11 precursor [Mus musculus]
    MNRKVTAIALAAIIWATAAQGFLMFKQGRCLCIGPGMKAVKMAEIEKASVIYPSNGCDKVEVIVTMKAHK
    RQRCLDPRSKQARLIMQAIEKKNFLRRQNM
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): MVA/IL-15/5Mtb vaccine
6. Cxcl9
  • Gene Name : Cxcl9
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 17329
  • NCBI Protein GI : 110626107
  • Genbank Accession : AC109603
  • Protein Accession : NP_032625
  • Taxonomy ID : 10090
  • Chromosome No : 5
  • Gene Starting Position : 92321330
  • Gene Ending Position : 92328078
  • Gene Strand (Orientation) : -
  • Protein Name : chemokine (C-X-C motif) ligand 9
  • Protein pI : 11.28
  • Protein Weight : 14046.52
  • Protein Length : 126
  • Protein Note : Also known as CMK; Mig; MuMIG; Scyb9; crg-10; BB139920
  • DNA Sequence : Show Sequence
    >gi|372099105:92321330-92328078 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
    AGACATTGAAAAACATGTTTATTTTGGAATTTAGTGCACTTTTACTGATATCACTACGCTTTCACGAAAT
    AGCTTCAGAGCACATATGCTAATTTGCTTTGAATTAAAAATTAGAGTACACATATGAAGTCATACTGTAG
    GCATATGCATCCAACATAACTATTTAGTAAAAATTACACAGGCTTTGGCTAGTCGTTATGCATACTACCC
    AGCTGTCAGATGCAAGGGTAGCTAACTAGTTAGGAAACAATTTTAGACTTTTTTTTTTTTTTTCCAAAGT
    AAATTACCGAAGGGAGGTGGACAACGTTTTGACTGTGCAATGTTGTTTGGATGGGAGTCCCACCAGCAGC
    ACAAAAACCACCTGCATGCAGCGCCAGGAATGGCAGTATTTGAGAAAAAGCATGCTCAAGACGGAACTTC
    TGCCCAGAGACATTCTTTTCTATCTAATTCATCAGACCTCCTCTCTAGAGCAGTTTCCAGCTCCCACACA
    GGCTAACTTGGCCCTGTCCACACTTCCACTCGCAGAATTTACTGCTTCCTGTAGCTCTTAATTCCTTTAA
    AAATATCTCTGTAAAGATTTTTATTTGCCGTCTGAGATTATGGGTGTATGTCATGGGGGCCTGTGTACAC
    CTGTGCAATGCTTGCAGAGGCCAGAAGAGAGAAATGGGTTCCCTGGAGCTGGAGTCACAGGTGGCCACAA
    GGCTCACGCACACGTGGGGGTGGGTGCTGGGAATGGAACTCTGGCTCCCTCTCCAGCCCTGATCTTTCCA
    TTTCACCACTCCTAGCCTGATGTGGCAATCCATACAATCATACACTGGGATTTCTGCAATGAGGGAGGGA
    AGGTGGCAAGGAAATTCATATTCACCTAAGATAAACTTGCTAAAGGATTTGGCAGCTCTTTTGTTGACTC
    CTAGGGAGCTTGTCCTGATCAAGAATGATATTAGAGGAATTTTGTTTTGAGTGAGGAAAGATCTAGTATT
    TGCCAATCAGTCAAACCTGTCTTCCATGTAAGCTTATGTTTCTAGATGGCCTGTGTAATTTTTCCTGAGC
    AGTCCCAAATATCCGCAAATGTCTTGAACATGTGATTCCTGTTTGTTAAAGACACGAGCCTCCTGTCACA
    AGGTAAGGCTCTTTTTCACCCTGTCTGGCTCTGTGCGCTGAAGATGTCTGAAGATAATAAGAAAGCTAGA
    TAATTTAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGGAGGGAGG
    GAGAAAGGAAGGAAGGCAGGCAATCAGGTGTAGTAGCACACACCTTTAACCCCAGCATTCAGGAGGCAGT
    AGAATACAGATATGTATGAATTTGAGACCAGCCTTGTCTACTTTGAGAGTTTTAGGACAGCCAGATATAA
    AACTTTCTTTAAAAAAAAAAAAAAAAAGTGGTAAGCAAGCAAGGAGGCCAAAGGTTAGTTAGGCAATTTG
    GAGCAGCCGGGGATTGGCCAATGCCTGGTGTGTAACAGCCTTTCTACCTCTCACACATACAATTAGGAAA
    TTATTCAAAACCAAGTTTTTGTAGGCACTGTGGAAGATTTAGGGGAATAGTCTGTGGTCCCTACTCTTAA
    AAACCCGTCTCGTTGGAAAAAGTAAAACAAACAAACAAACAAATCCAATTAGATAGCAGCGAAGTGTTTA
    CTTGTGAAACAGGAATAAATTTAAGAGAATTTGAGTAGCCAGAAAATGTTCTTTAAATTGGTTAAGTTTT
    TGCACTGTTAGTGAAGGACCTGGTCCCTGCAACACCAAGTGTTCTGCCACTAAGCTACAGCCACAACCTT
    CTAGGAGAGTTTTATTGAAGAGGCAAACTTAGAATAAGCTCTGAAGATGGGATCAAGTTAATAATGAAAT
    CCCATGGTCTCGAAAGCTACGTGGGAGGTTCTGAAGGCCAACTCTGGTGGTCTCCACTGTAGAACCCCAA
    AACCATTAGCACCATCTCTGAGACACAATGTGTGCAGGGGCTCTAGGCTGACCCAAATGCAGCTGCCGAG
    GGCCTCTCCAGCTTGGTGAGGTCTATCTAGCTCACCAGCAAACAGACAGGCCTGGGATTTGGTGACGTGA
    GCCTCAGAAGTCTTCGGAGGCTGCCAAATGTGTGGCTCTGGAAGCCAGACAGCTGTTGTGCATTGGATAG
    CTATGAAGGAAAGGGACACTTGCCCAGATCTGATGTTATCACGACTAGGCTCAAGGGCGTGATGAAATTT
    TGTTGGCTGTGTAGAACACAGAGTTTTTGCCTCTAGCAATTGTTACTATTGGAGTTTTGCAATTAACTTC
    CTAAAGTTAGTTATAATTTCAAATTAACACTTTATGTTTTGTAGTTGAGTTAATGTATTAAAAGCCTATG
    TATTAAAGGCTGCTCTGCCAGGGAAGGCTTTTCAGTACAATCTAAAACCCATTAAGATTCAGGGTGCTTG
    TTGGTAAAGTAATGGTCTCTTATGTAGTCTTCCTTGAACGACGACGACTTTGGGGTGTTTTGGGTTTTCT
    GTTTTTCATGTTCTTTTGATGTTTTTTCCCCCTCTTTTGCTTTTTCTTTTGGCTGATCTGCAAGAAATGA
    GAAAAAGGCAATGCTTTCCAATGTTGTAGTTTCTCCCCTTCGAGATGGTCCAACTCCTGAGTGTGATCTC
    TCCTTATCCTCAGAGCCAGCTCACGTCGCTGCTCTCTCAGAGGCGCTGGCCTCATTATCTGCTGCCCCTA
    CATTAAAGAAGCTACAGTTCCCTCTTTGCTTGTTTGTCTGTTTTCTGGCATCTTGCTGTCTTTCATCTTC
    TCCCTTCATCTCCCACCTGGGCCAAACACAGCCCTTTGGGGCTGGCTGGTAGCGTTATCACTCACTTTGC
    ATATTAGCCAGTGGTTCTCAACTTGTATGTCGAAACCCATTCGGGGGCACATAGCAGATATCCTGTGTCT
    CAAGTATTTACATTATGATTGTTAACAGTAGCAAACATTACAGTTATGAAGTAGCAGTAAGATAACTTTA
    TGGTTGGGGGTCACCTCAATATGAGGGACTCATGCATTAGGGTCTCAGCATTAGGAAGGTTGAGAACCAC
    TGAAACTGGACAAGGCTAGGCGAGTGAGTAGCCTGAAGCTTAAGGGCTAGGGTCTAGGGCAGACCCAGCA
    GTTAGGTGTGGGGACCCAAGCTCCCCACCACATACCCCATGGCTCTTCTCAAACCCAGCTCCTTAGCTAT
    CTCACAGCTCTTTTCCCTTTTTCCTTGGCTCCTTTCTCATCTCAATCCCTTTGTTCTCTGCATTTAAATT
    CCAGTTACATTACTTCAGACTCACTGAACAGACTTTACTCAAAACCACCAACGCCCACACTGTACTTAAT
    TAACTGCCTGGTAAATGTCTTATCCTCAAAGATCCAGATCAGAGATCACTTGATAGGAGAAGTCTACCTT
    AATACTTTCTATATTGTCTCTTGGTCAAGTCAATTACCCTTTCCATCATAATTCTCAAAGTTCTCATGTA
    CACACTATTTATTCTAAATGTCTCTCACCACGTCTGTTGATTTTTATACATTGTTAATGACTATCTGCTA
    TATGGGGGAGGGGTGATAGCTTATTAATTTTGAATCCTTAGTGTCTTACTAGTTGATGCTATTCAAAAGG
    ATGGAAAATGGGTCCTTAATGGGTGTGCCCAGGATCTGCTGACTTTGTGAGCTGACCTCTGAAAGAAAGC
    CATTTGGGTTCCTCCCCCACCAACCTTCTTTTCCCATTCTTTCATCAGCTTCTTCACATTTGCCGAGTCC
    GGATCTAGGCAGGTTTGATCTCCGTTCTTCAGTGTAGCACTGTGCCAAAGAAACACCAGTGAGCTTTCTC
    CCCCCAGTAACAACTTCATAATTCTTAGTAGAAACAGTGGAGGAAAACGTGCATTTTATTGAGTTTTCCA
    GTGAAACCACTTAAAATGTGGCAAAACGTCTTCCTAAGATTAACAGGTATATTTTTAAAAACTGTAGCAG
    ATTTTTCAGGAGAAACCTCAATTTCTAAAAATTAATTTCTCTGTATAGCTTGAGAATAATAATGGCTATT
    CTATATAGAATTCCTTATTTTAGTTGTCAAAGGTCAAATATATTACATACATGTATGTATATATATATTT
    ATATCCTTTATATATGTATGTGTATATATTTATATCCTTTATATCGTATACATACATACACACATATAAA
    TATATTATATAATATTATATATCCTTTAATTTCATAGTTCTGATTTCTAAGAGGCTTGGAATGGTGGTTT
    ATTCCTGGAATCCCAGCATTCACGTGGACTGAAGTCAGCCTTGGCTACCTAGCAAGACTCTGTCTCAAAA
    CAAAACAAACACGAAATTCTAGTCTCTAGAAAAGAATTACTAGGAAAGAATTAAACACAATCCTACAACA
    GTAATAGTCTGACAACAGTGAATTTTTTTTAAACATAACCGTTGTTTCTAGCAATGGGCCATGTGCTATA
    CAAATTATGGCTTATCAATATGATGTAAAACAGCTTAGCCATCAAAAGTCATTATTAGGCCAGGGAGTTG
    ATGTGACTGCTAAGCATACTTGCCTCATAAGCCCAGAGAGCTGAGTCAACCCCAGAACCCCAAATTAGGA
    CAGAAGAGAAAACCGACTCCACCAAGTTGTTCTGATTGCTGTATGCATGCTGTGGGATGTGTTCTTGAAC
    ATACAAAGTATGGGTTAAAATTTATTTTATTTTTAACTTCTTTGTGTCTCTGCATGTGTATGTGTACTAG
    TGTGCAGCTGACCACAGAATCCAGAAGAAGGGATAGGATCTCTGGGGTGGAGTTACAGATGGTTGTGAGT
    TGTACACTGTGGGTAATAGAAACCAAACTCGGGTCCTCTTAACCTCTCAGCCATCTCTCTGCAACCCCTC
    AAAAAAATTTTAAATGCCGCTTTTAGGATTCAGGGTTAGGGTTAAGTAGAGAGAGAGTACATGCATAGCC
    TGTGCAAAATCCTAGGTTCAACCCTAGCACCCCCAGACAACTGACAAGTATGGCATTTTTAGAGAAAATT
    AATTGTCATGAGAAACTGTCGTTGTTATTCTGTTAAGGAAAAAACAAATTACAAATGGCAACTTCTCCTC
    CCCTGTAGGGAAGATGTCCAGGTTCCTACACTCTCAGATGGTACCTACGTACCTATATGCCTTACTTAAG
    ATAAAGTCTAATGTGTAAACTAAACAGACTAAGGTATTAACCGCACATTTTATTATTTAAAGATATGTCA
    TCTTCCATTTTCAGTTTCCAGAAGAAATTTCTTATTTTGAAGAGGAAGAAAGAAAAGGAGTAGACAGATT
    TATGGGGGGGCCTCCTGCCTACATGAACAAGTAAGCAAGCTGAGGGTGAGAATCCCACTAAGTCCAGTGT
    AGGATGCATGCGTTGGGAGCATTTACTTACATGATTTCAGTTTTGTTGCAATTGGGGCTTGGGGCAAACT
    GTTTGAGGTCTTTGAGGGATTTGTAGTGGATCGTGCCTCGGCTGGTGCTGATGCAGGAGCATCGTGCATT
    CCTTATCACTAGGGTTCCTAGGCGGAAGATAATGCACATTACTTAGAATGACATCCTTTCGAATGAGGCT
    TGTCTCAGTCACTATTGCTGCGACTGAGTGTCTGTGACTGCTTTTCAAGGTAGGTGGTATTTCCATTCTG
    TATACCAGGAAGCCAAGGTTAAGGGCAAAGTCATTTAGCAAAAGGGAGCAGGGGAAGAACTTCATTTTAG
    GTCATTTAGAGTTCTTAACAACATCACTTCACAACCTTGGAAGAAGAGAAAACCTCCATCATACTTAAAT
    CCCTACTGTTAGACACACATATATGCAACCTGTCAGTACTACGGGGACCGGTTTCTTAAGCAGTTCCTCA
    ATCTACTTCTTGCTACTGGGGTCATGGAGTTGATGTAGGTGTGGACAGAAAGCTCTACTTACAACAATTG
    ATTACCATTTCAATCCTATTCCCTCTCATCCTCACTGCTCTGAAAAGTCTCTTTAAACCTATGTCTCCAT
    CCCTTATTGCTTACCCAGGTCAGTGTCTATTTACACAGGTTTTGCCAACACACAGTAACTATGGCACACC
    TACTTTACACACGTGGAAAACGCTCTCTAGGAAGGTTTTTTTTTTTTGTCCGAAGTAAGCAAAGTCATGA
    ATAAGGAGGTCAAAGGCAATCTCTCTGACTTCTGAATTCTCAGTTGGTTAACCACAGACATTCTTATCAG
    TGCACAGCATCGGTTGAGGAAAGTGATGCAGGAGACAACTTAGAAAAGGATCTCTTATGTCACTCTCTGT
    TGCCAGGGCTGCGGATGAAATATTTCACAGGATAAATTCGAAGTAAAGCACCCAGAATCCCCTTTACATC
    TGAGAAATGTTTTTTCATTATTAAAACATTTAAATATGCTTTGAACCCTCATTGGGAACTCACAATACCT
    ACGCCAAGAAGGCATATTTAACTTGGCAGGTATAGATTACGACCTTTGGATAACTGCTCACAGCATCTAA
    CTGCTTTGATGACCAAACAGTATTACTTTTTATTGCATGCAAGACATTCAGCTTTATCTGCTGGCCTGAG
    AAGGCTTTTACGGTGATTCAGTCCTCAGAACTTACTAACGTAAAACTCCTCACCTCGAACTCCACACTGC
    TCCAGGAAGATGATGCCCAAGAGGAAAAGAACAGCGGACTTCATGGCAGAGCTGAGTTCTATTGAGTCAC
    TGTGTTGGAGTGAAGTCCGAGAATGTCTT
  • Protein Sequence : Show Sequence
    >gi|110626107|ref|NP_032625.2| C-X-C motif chemokine 9 precursor [Mus musculus]
    MKSAVLFLLGIIFLEQCGVRGTLVIRNARCSCISTSRGTIHYKSLKDLKQFAPSPNCNKTEIIATLKNGD
    QTCLDPDSANVKKLMKEWEKKISQKKKQKRGKKHQKNMKNRKPKTPQSRRRSRKTT
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): MVA/IL-15/5Mtb vaccine
7. Defb3
  • Gene Name : Defb3
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 27358
  • NCBI Protein GI : 7304997
  • Genbank Accession : AC113099
  • Protein Accession : NP_038784
  • Taxonomy ID : 10090
  • Chromosome No : 8
  • Gene Starting Position : 19293360
  • Gene Ending Position : 19295338
  • Gene Strand (Orientation) : +
  • Protein Name : defensin beta 3
  • Protein pI : 11.07
  • Protein Weight : 6827.63
  • Protein Length : 63
  • Protein Note : Also known as BD-3; MGC129397
  • DNA Sequence : Show Sequence
    >gi|372099102:19293360-19295338 Mus musculus strain C57BL/6J chromosome 8, GRCm38 C57BL/6J
    CCAGGCTTCAGTCATGAGGATCCATTACCTTCTGTTTGCATTTCTCCTGGTGCTGCTGTCTCCACCTGCA
    GGTGAGTCAGGGGAGTAGGGTCATCCCACACTGATAGGAGAGACAACACCCTACATCAGGTGTGTGTGTA
    TGTGTGTGTGTCTGTGTGCCTATGTATCTCTGTGTTTGTCTCGGAGTCTCCCTCTCTATGTATCTTTCTG
    TTTCTCTGTGTTTGCATCTCTCTCTGTGTCTTTGTGTTTCTGTGTCTCTATGTTTGTGTCTGTCTGTCTG
    TCTATCTCTCTCTCTTTCTGTGTATGTGTGCATGTGTATACCTTTATGTCCCTTAATCTTCGTGTGTTGG
    TTTGTCTCCCTCTCATTTTATGTGTGTGTGTTCCTCTGCGCATGCATCTGTCTTTCTGTCTCTATGTGTA
    TATGTCTGTCTTTCTGTGTATAAGTCCATATCTATGTTTCTGTCTGTGTCTTTTTCTCCTTTTTACATAT
    ACCCTCCTTACTTTTGCATTTTTCTTTCTTTCTTCTAAGCATTTTAGAAAATGTGTATTGTTGACTACTA
    ACACAAACCAAGGACTCTTTCTTTTTCCCTTGAAGGAGTCTAAGGTAGACACATGCTCTGCTCATCTGCA
    GCAATTGACTTCCATTTCCTAACTCCTGATGCTAAGCTTAAGATTATCAAATGAGTGTCTTATAGGTAGC
    TTAAAAATAGTAACATGAAGAATGTTTGATTCTTACATGAACAGAAAGGTATTAATTTAGAAATCTTTAC
    ATATAAGGAGTAGAGTCTCTGCTTACTCTAGAGAACTCCTCAGAATAACCAGCCTAGCCTGTTTGAGAAC
    TTCTGCTCCCCTTAGCCCCATGGAATACCCCTGAGCACACAGTGTCTACAGGGTTAATGAGGTTCATAGC
    ACAGGGGAGGCTATTAAATCCTGTCTCCCAAACTTTTGCTTAAGTGTATTTAGTGGAAAAAATTTATTTT
    CTGTAAATTATGTGTATATGTATTGATTTGTATATGGGTATGTGCCAAGTAGTACAGTACTCACAGAGGT
    CATAAAAGGAAATCATATCTCTCAGAGATGGAGTTATTTATAGGCACATATGGCCTGCCTGATATGGTAC
    TAGGAACTTATCTCAGGTCTTCAGCAGGAGCAGTATGCACTCCTACAGGCTGAGCCATCTTTCCAGGCTC
    CAAGCTGTGTTTCATACTTGACTTTAGACAAACAGAATAATGGGGTTAGACAACTCATGTGAGGGAGCTA
    AAACAAGTAATGAGTTGGAGAGGAATTGATGAACACATCTTAGGCCAACCTGTGGCCTCTATATGTGTGT
    GCACACATGCACATTCATGTGCACAGTCACACTCAGTGTCTGTTTCACATGCTAGAAGTAGGGGCTTGTG
    TGTGGAGAAGCAAAGGCAGGGCTGCTAAGAAGTAGCTAGGGAGTCACACAAAATCTTTCCTGAGCTACTG
    CATGCTGGCTTTGAATACTCCAATCAGTCAGGGGTTGAGCAGATCCTTCAACATTTGGGTCCTAGGAATA
    CCAGCACCTCCTCTACACTGCAACTGTGCCTGTCTCGAACAAGAATTGGATTCAGCCCTGCTAAAACAGA
    CATTACTGTTTGGGCAGAACAATCTGCTGGGATCTACTTATCATAAGAACACATTGGTATGTATTTCTGA
    CCTACCACTTTCAATTTTAATGTATGGTTTTTCTTACTTATCTTTCAGCTTTTAGCAAAAAAATCAACAA
    TCCAGTAAGTTGTTTGAGGAAAGGAGGCAGATGCTGGAATCGGTGCATTGGCAACACTCGTCAGATTGGC
    AGTTGTGGAGTTCCTTTCCTCAAATGCTGCAAGAGAAAATAGAGAAGACCAAGATCCCGTTAAACAAAGA
    AAACACCAGAATTTGCTCCTCCATGAAGATGGACAAATCTTAAACCAAATTAATATTCCTTGATTAAATG
    CAAACAAGTGCTCCCTAGC
  • Protein Sequence : Show Sequence
    >gi|7304997|ref|NP_038784.1| beta-defensin 3 precursor [Mus musculus]
    MRIHYLLFAFLLVLLSPPAAFSKKINNPVSCLRKGGRCWNRCIGNTRQIGSCGVPFLKCCKRK
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
8. Defb4
  • Gene Name : Defb4
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 56519
  • NCBI Protein GI : 9789929
  • Genbank Accession : AC116394
  • Protein Accession : NP_062702
  • Taxonomy ID : 10090
  • Chromosome No : 8
  • Gene Starting Position : 19198703
  • Gene Ending Position : 19201546
  • Gene Strand (Orientation) : +
  • Protein Name : defensin beta 4
  • Protein pI : 9.22
  • Protein Weight : 6929.73
  • Protein Length : 63
  • Protein Note : Also known as BD-4; 2310001F05Rik
  • DNA Sequence : Show Sequence
    >gi|372099102:19198703-19201546 Mus musculus strain C57BL/6J chromosome 8, GRCm38 C57BL/6J
    TGCATCTCTGCATCTCACCAGGCTTCAGTCATGAGGATCCATTACCTTCTCTTCACATTTCTCCTGGTGC
    TGCTGTCTCCACTTGCAGGTGAGTCAGGGGAATAGGATGGTCCCACACTGATATGAGAGACAGCACCCTA
    CCCGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGCTTTATGTGTCTCTGT
    GTATGCCTCTTCGTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTT
    CTTTCTTTCTTTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTCCT
    TCCTTCCTTCCTTCCTTCCTTCCCCCCTCTCTTTCTTTCAAGATTTATTTATTTATTTTATGTATGTGAG
    TACATTCTTCAGACACACCAGAGGCACTAGATCTCATTACAGATGGTTGTAAGTCACCATGTGGTTGCTG
    GGAATTGAACTCAGGACCTCTGGAAGAGCAGTCAAGTGCTCTTAACCACTGAGCCATCTCTCCAGTCTCC
    TCTTTGTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTG
    TGTGTGTGTGTCTGTCTGTCTTTGTCTCTGTCTGTCTCTCTCTAATCCACTTATTGCCCTCCTTTTTTTC
    ATTCTTTCTTCTAAGCACATTTTAACAGTGTGTATTGTGTGATTGCTGAAACAAACCAAAAACTCTTTCT
    TTTTAACTTGAAGAAGCCTAATGTAGACTCACTCTGAGCTGATCTGCAGTGCCTGACTTCCATTTTCTAA
    TTCCTGGTGCTAAGCTTAAGATTATGAAATGAGTGTCTTATAGGTAGCTTAAAATTTATAACATGAAGAA
    TGTTTGATTCTTATCTGAAAAAGTTATTCATTTAGAAATCTTTATATTTAATGAGTATAGAGTCTCTGCT
    TGCTCTAGAGAATCCTCCAGAATAACCAGCCCAGCCTCTATTTGAGAACTTCCTTTCCTCCTACCTCTAT
    GGAATACCCTTAAGCATACACATTCCACAGGGGTAATGGGGCTCATAGCTCAGAGGGGTTATAAATCACA
    GCTTCATAAACTCTGCCTACATTTTATTTAGTGGAAAATGTTTATATCCTTTTAAATTATGTACACATAT
    ATGGATATGTGCCAAGCAGCACAGTACCCACAGAGGTCATAAAAGGACATCTAATCTCTCAGAGATAGGG
    TTATATACACATATGGGCTGTCTGATATGGTACTAGGAATTTACCCCAGGTCTTCTGCAGGAGCGGTATG
    TACTCCTAACTGCTGAGGCATCTTTCTAGGCTTCAGATTGTATTTCATCCTTGACAAACAAAACAAAGGG
    ATTCACAACTCAAGAGAGGGAGCTAAACAAGTAAGAGAGAACATGAAATGAATGAATGAATGAATGAAGC
    AAGGACCTCAAACTGCATCAGACAGAAATGATATAGAGAGGAACATAATTTCACTAATTTTGTTGAAGGG
    TTCTGGTTTGGAAAGGAGCATCTGTGTTCCATGAGATACACAGACACTTACTCTGCCTGACATTTAGATG
    AATGCAAAGAACAAAGGAGGGAGAAGAGGGAAGGATGAGATTAAGACTGGATTTTAGACATTTGAAACTA
    TCAAAAATAAGATTTTTTACTTTTGATTTCTATTGTAAATTACTAAGGAATCAAGTTATCATTAAGCAAG
    TCTTATACATGGAGATTTGGGGCCAATACTTCATTTAAAATAGGTACCTGGAAGTTAAAATACCTTAATT
    AAAAATAGGAAGATGGCTCAGTGATAAAAAGTGCATGTTGTGCAAGTATGAAGATCAGAATTTGGATCCC
    AGTGTCCATGAAGAAGCATAGAGGGCTTCTGGTGTACATCTGTAATTCTAGCACAAAGAAGGATCCCACA
    GGCACATTGGCCAGCCAGCCAGCCAGACCTCTCAATGAATTCCAGTTCACTGAGACACTCTAGATCAAAA
    ATTGAGGCAGAGAACAATTGATGAACATATCTTATGACAACCTCTGGCCTCTATATGTGCCTGAAAACCT
    GTTTGTACACATTCATACTCAACAGCTGTTTCTGATACAAAAAGAAGGATTTTTTTATGTGGAAAAGGAG
    AGGCAGGGCTGCTGTGAAGAGGCCAAAGACTCACACAGAATCCCTACTGAGGTCTAGCCTGATGAACCTG
    AAAATTCCCATCAGTCAGGGGTAGAGCAGACCCTTCAACATTTGGGTGTTAGGAAAACGAGCACTCCTTT
    ATACAGCACCAGTGGCCAGAACTGGATTCAGTTCTGCTAAAACAGACAACCCAGTTTTGGGGGCATGGGG
    ATCCTGCTTGGAATGTACTCAAGGTAATAGCACACTGGACTGTATTTCTGACCTGAGGCTCAACTCTAAC
    ATGTGTTTTTCTTACTTATTCCAGCCTTTACCCAAATTATCAACAATCCAATAACATGCATGACCAATGG
    AGCCATATGCTGGGGTCCGTGCCCTACCGCTTTTCGACAGATTGGCAATTGTGGCCATTTTAAAGTCAGA
    TGCTGTAAGATAAGATAGATAAAAAGACGAAGAACCAGCAAGATGAATAAATTGCCAGAATTTGCTCCTC
    CATGAAGATTCACAAATCAAACCCAATTAAAGTTCTTTGATTACCTTCTCTAACTCCAGTTCTAGGGGAT
    GCACACCTTCTTTTGACCTTTATAACAACCATTTGCAGAGTACATACAGAAAAAATACCATACAAATAAA
    ATTATAATAATAGTAATAATTATAATTTTAAAAATAAAGTCACT
  • Protein Sequence : Show Sequence
    >gi|9789929|ref|NP_062702.1| beta-defensin 4 precursor [Mus musculus]
    MRIHYLLFTFLLVLLSPLAAFTQIINNPITCMTNGAICWGPCPTAFRQIGNCGHFKVRCCKIR
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
9. Ebi3
  • Gene Name : Ebi3
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 50498
  • NCBI Protein GI : 7657052
  • Genbank Accession : AC154235
  • Protein Accession : NP_056581
  • Taxonomy ID : 10090
  • Chromosome No : 17
  • Gene Starting Position : 55952622
  • Gene Ending Position : 55957021
  • Gene Strand (Orientation) : +
  • Protein Name : Epstein-Barr virus induced gene 3
  • Protein pI : 10.35
  • Protein Weight : 23864.43
  • Protein Length : 228
  • Protein Note : Also known as EBI-3; IL-27; interleukin-27 subunit beta
  • DNA Sequence : Show Sequence
    >gi|372099093:55952622-55957021 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J
    TGACTTCTCCCTTTTGGGGCCCTCCTTCTACCTCAAGGCCTTTCCCCACACATACACACAGCAGCTGGTG
    ACAGAATCAGGGTTCCCGTCACCATCATCATCTCCTGCCCCATACACTGGACAACTGAGCCACACTGGGC
    AGGTCCTTCCCTGGGGCCAGGTTCCCTGTGTGAGTCCCCTGTCCTTCACCCTCTCTCTGATGGGTCACTA
    ACTCGGATCCAAGGAACAGAGCCACAGAGCATGTCCAAGCTGCTCTTCCTGTCACTTGCCCTCTGGGCCA
    GCCGCTCCCCTGGTTACACTGAAACAGGTAGGTGACCACAGGGGGACTGGGGCACCTGGGCGGAGGGAAC
    TAGTCCTTCCCTTTCAGTTTAAGGTCATATCTACCATCAGCTGGGAACTCACACCTATACCCGTTGGACC
    TGTTCTCCTTCTACTACAGGGATGGGGAAACTGAGGCATGAAGCCTCAAAACCTCATTTATCCAGGCAGA
    GGCACCCTTGAACTTAGACATTTGTGGAGCTAGACAGTTGACTCTCAGAAACCCGAAACCTGAAGCCTCA
    CCTTTGTGACTCAGCAGCTGTAAAAGAAAAGAAAACGAAAAAGAAATGTAGACAATACTGGGTATAGTGG
    GGCACACCTGCCATGAAGCTCTCAGGAGTTCAGGGTCATCCACAGTCACATAGGAGGAAGCCAAGTATCA
    AGAGATTCCAATGTCCTGATCCACTCAGGGTAGGAGGCAGCCTAGGCCCCTTGGGAACCTGCTTCTAAAG
    CAACAGAACAAGTGTGGGGTGTGGGTAGTCTAGGGAGTTCTTCAGAATCCACAGTTCAAGGCCAGCCTAG
    GCTATGTGAGACCCTCCAGAAGGAGGAAGAGGAGGAGGAAGAAGGAAGAGGAGGGAGATGGAGGAGAAGG
    AGAAAGGAGAGAAGGGAAATTAGAAAAAAAGAAGAAAAAAAAAAAAAAACCTCAGGCCAGGCAGTGGTGG
    CACACGCCTTTAATCCCAGCACTTAGGAGGCAGAGGCAGGTGGATTTCTGAGTTCCAGGCCAGCCTGGTC
    TACAGAGTGAATTCCAGGACAGTCAGGGCTACACAGAGAAACCCTGTCTCGAAAAACCAAAAAAAAAAAA
    AAACAAAAAAACAAAAAAACCTCAGTTTTCTCATCTAGAAATTGAGCTAAGAACCTTGGGGTTTGTAGTA
    AGTATAGGAGCTCATGCTTCCATGCATAACAGGTCCTACATGGCCTACGGAAGCTCCTGTTCCTTTGTCT
    CCTCCAGCTCTCGTGGCTCTAAGCCAGCCCAGAGTGCAATGCCATGCTTCTCGGTATCCCGTGGCCGTGG
    ACTGCTCCTGGACTCCTCTCCAGGCTCCCAACTCCACCAGATCCACGTCCTTCATTGCCACTTACAGGTC
    AGAAAATGGAAAGGGTTTAGGAGGAAGCCAAGTATCAAGAGATTCCAATGTCCTGATCCACTCAGGGTAG
    GAGGCAGCCTAGGCCCCTTGGGAACCTGCTTCTAAAGCAACAGAACAAGTGTGGGGTGTGGGTATTCTAG
    GGAGTTCTTCAGAATCCACAGTCCCCAAGTCCCCCTGAGCATATCACCCCCTGAGCGTGACCCACAGTCA
    GCTGTGGGTGAGCTGGGGCCTCACGGGCACCCAGGTTCCTTTCGAGATTCACCATGGTGCTTCCCATGCA
    GGCTCGGTGTGGCCACCCAGCAGCAGAGCCAGCCCTGCCTACAACGGAGCCCCCAGGCCTCCCGATGCAC
    CATCCCCGACGTGCACCTGTTCTCCACGGTGCCCTACATGCTAAATGTCACTGCAGTGCACCCAGGCGGC
    GCCAGCAGCAGCCTCCTAGCCTTTGTGGCTGAGCGAATCAGTGAGTGTCTGTCCTCCCTGCCCCTCTCCA
    CCTGCTCCATCCCGTGGCTCCTCCCTGCCGCCGCCCCCCTACCCCCACCCACCTCTTGCTTTGTCCCTGC
    AGCTTCCATCCTTTGGCTCTGCTCCCGCCTCACAGGCTTCCTGTCGATGCCCCGGGGCCTTTGCATTGCT
    TCCTTCCTTGGCTTTCCTGGGTCTCTGCTCCAATGTTGGCTCCTCAGTGAGGCCTCCCCAAAAGTATATC
    ATTAAACATAAGCAATAGTCACAGTTAAGAGAGGATGAAAGCCAAAGGTGGTGTACAAGTCTTTAATCCC
    AGAGGTAGGCAGATCTCTGAGAATTCAAGGCCAGCCTGATCTACAGATCATGTTCAATGCAAGCCAGGGT
    CACTCCTGAGCACCTGTCTCAGAAAAGAAAGCCAAGAACAAAAGCTGATGCAGGTCGGTGAAAGGCTACA
    CAGCTCTCGAGGGTTTAAACCTGAAGTCTCGACATGATCGCCCAACTCCAATCCTAAGGATCTGCTCAAC
    AGCAATAAAGCTGTTCCTCCCTACCAATCCTTGGCAACATCATTGCTGGCATCCAGAAGGCCAGTGCCCC
    GTGCGTTCCCATGGAGCGATGGGCACCCAGTGCTTATCTAGCGTTACTCAGCCCTAACAAAGAGAGAGTG
    GGGCTCTGGCACCAACAGCTGTGGCGGGACCTTGAGGACACTACGCTCAGTGAGCAACCCAGACTCAACC
    CACCCAGTGTGGGCTGCAGTACAGAGGAGTCTAGAGACAGGAAGCCTGAGTGCTGAGGACAGAGAAGGGT
    AGGAAGATGGCTTGTGTTGTCATGAAGGAGTGTTCCATAAGTAGGTGGAGTAATGAAAACGCTCTCTGCA
    GATGCACGGGATGTCTTCTAGCAGAGACTTGTGCTCTCAGAATTGTATCTCAGAAAAGCCCTTCAGCCTA
    CTGGAGACTTGCACTTTTCTTTGCCTGTGCAACCCTTGTGACTTCTATCAGCAGTGATGAGAGCGGTCCA
    GCAAATGGTTTTTCCTTGAGCAGATAGACGAATGAAGGAATAAATGAATGCCTGCATGACAGATATCAAG
    TGGATGAGTGAATGATTTAGTAATGAATGAATGCATAATGAGTGAATGAAGGCAGAAATGACATGAGTGC
    ATGCACACGAATGACTTAATGAATGAACGAATGAATGCATGAATGACTTAGTGAATGAGTGAATAAATGC
    ACAACTGACATAATGAATGCATAATGAATACATGAAGGCCATGAGTAAATGAATGAATGACATGAGTGAG
    TGCATGAACGAATGAATGACTCAATTAGGGAGTGAATGAATGAATTCACAAAGGATACAGTGAATGACCT
    AATGACTGGGAAATGGATCCCTGAACGAATGAGTGTATAAACGATAAGCGCAAATGAATGAGTTAATGAA
    TCCAGAAACAAATCTGCACTTGAATCAACCAATAAATAAATGTGTGAGTGAGATGGTGAGTAACTGAGTA
    GAGGAATGAATTAGTGAAGAATTTAGTGAATACGTGAACGAATGAAGTGTGAGTATGAATGAACGGCAGG
    ACGGAGCAAATCCATGAATAGCTGTCTGAGGGCGAAAGCGAACATGTGAATGAATGAGAGTCACAAACGA
    AGACTTAGTGTGTGAGGGATGAACCGGCAGAGCGGACGCTGACCCCGGTCCTGCTCCCACCAGTCAAGCC
    GGACCCTCCGGAAGGCGTGCGCCTGCGCACAGCGGGACAGCGCCTGCAGGTGCTCTGGCATCCCCCTGCT
    TCCTGGCCCTTCCCGGACATCTTCTCTCTCAAGTACCGACTCCGCTACCGGCGCCGAGGAGCCTCTCACT
    TCCGCCAGGTGAGCTGCGCGCGCAGGGGGGCGTGGTCTGGAAGAAGGAGGAGCACAGAGGTGGATGCTGA
    GTGAGACTGGGTCCCACTGGGTAATCTCCTGTCTCCGCCTATCGCCTCTGTTGGAGCGTAGAGATTGTTG
    GTGTGATTCAGCAATAGACTTTACCTGCTTGCTGGGAATTCGAACTCACTGCAAGCTCTTTACCCACCAA
    GCCAGCTCCCCAGCTCCCCAATGCATTGTGGTCCTTCCCACCTCCTCCTTCCAATGGCCTGACACCCCTT
    CTCCCCACAGGTGGGACCCATTGAAGCCACGACTTTCACCCTCAGGAACTCGAAACCCCATGCCAAGTAT
    TGCATCCAGGTGTCAGCTCAGGACCTCACAGATTATGGGAAACCAAGTGACTGGAGCCTCCCTGGGCAAG
    TAGAAAGTGCACCCCATAAGCCCTGAAGAGGGACTGGATCCTTGATATCAGACCCTGATGTCGTCTACTT
    GAGGGTGGCAGGGTGGCCTGTCCTGAGTCTGAATACTGACTTTCCATGTACTGGGCTGCTCCGAAGCACT
    GGATAATTCACTTGACTTCTTCAGACCTCAATTTCCAACCCTGTGGGATGATCTTTCTTCTTCTGCCGGT
    GCGGGGCTTTGTGAACTTTGTGATTTAATTAGAAAGACGGATTAAATAAGAATCATCATC
  • Protein Sequence : Show Sequence
    >gi|7657052|ref|NP_056581.1| interleukin-27 subunit beta precursor [Mus musculus]
    MSKLLFLSLALWASRSPGYTETALVALSQPRVQCHASRYPVAVDCSWTPLQAPNSTRSTSFIATYRLGVA
    TQQQSQPCLQRSPQASRCTIPDVHLFSTVPYMLNVTAVHPGGASSSLLAFVAERIIKPDPPEGVRLRTAG
    QRLQVLWHPPASWPFPDIFSLKYRLRYRRRGASHFRQVGPIEATTFTLRNSKPHAKYCIQVSAQDLTDYG
    KPSDWSLPGQVESAPHKP
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): MVA/IL-15/5Mtb vaccine
10. Fth1
  • Gene Name : Fth1
  • Sequence Strain (Species/Organism) : Cavia porcellus
  • NCBI Gene ID : 100379547
  • NCBI Protein GI : 290491181
  • Protein Accession : NP_001166318
  • Taxonomy ID : 10141
  • Chromosome No : Un
  • Gene Starting Position : 9448211
  • Gene Ending Position : 9449938
  • Gene Strand (Orientation) : +
  • Protein Name : ferritin heavy chain 1
  • Protein pI : 5.3
  • Protein Weight : 20567.49
  • Protein Length : 182
  • DNA Sequence : Show Sequence
    >gi|347623380:9448211-9449938 Cavia porcellus strain inbred line 2N unplaced genomic scaffold, Cavpor3.0 supercont2_65, whole genome shotgun sequence
    CGCGGTCTCCTGCTTCAACAGTGCTTGGACGGAGCCCGGTGCTCGCCTGCCCTTACCGCCGCCCTCACCG
    CCGCCGCAGTCTAACCTCGCCCGCCGCCACCACCACCACCGCACCGCCGCCGCCGCCATGACGACCGCCT
    CGCCCTCGCAGGTGCGCCAGAACTACCACCAGGACGCCGAGGCCGCCATCAACCGCCAGATCAACCTGGA
    GCTCTACGCCTCCTACGTCTACCTGTCCATGGTGAGCGCGTGGCGTGCAGCCCGCAGTGGGCGCTCGGGG
    TTGCCTGGCGACAGCGCAAGCTCCCCAGGGACGGGAGTGCGCGCCTGGATGTAGGAGAGGCGGCGGCGCC
    ACTGACCCCCCTCGGCTGTCTCCCGCCGGGGCGTGCACTTTGAGGGTGCAGCTGGAGAGCTCGCGAACCT
    GTGTAGAAGATACCGTGCAGGGCGAACGCTCAACGCACTCCCTCTAGACAGGAGGATTTCCCTGTAAACA
    CTGCTGGAGGTGATTTTCGGTGCTAGTTTGAAGCTTCTTGAAGCAGCTCTCAGCGACCAAGTCCCAGTTT
    TTTGACACAAGATCTCACTGTGTAACCCAGGCTGCCCTTGAATGCACAGTTATCCTCTTGCCTCAGCCTC
    CTCAGTGCTGGGATTACATGCGTGCTCTATCAGTTTTCTAGTTTAGAGTTAAGAGCCTAGTCCCTTTCAG
    GAACACATCAGGCCCATTCAGCCCTGGCCCCTCTGGTTGTGTGGTGAGAAGGGCAAGCTGGACAGGCAGA
    GGACACTCACCACCTCCCCTCGCGACAGCCATCTCCCTTCCCATGGGAACCTTCACTGGACGGTGATGTT
    TATTTTCAGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTCGCCAAATACTTCCTTCACC
    AATCTCATGAGGAGAGGGAACATGCAGAGAAGCTGATGAAGCTGCAGAACCAGCGCGGTGGCCGGATCTT
    TCTTCAGGATATCAAGGTGAGTGGGGAAGGTCTGGATGTGAGAGACTCTAGGGGTCCTTCCCTCCCCTCC
    ATGTGCAGCTATGGCTGGGGCTCTCTGCCTCTGTGCGTGTCAGGGCCACTTTATGCTAATGTAGGCACTG
    AATCTGCAAGGACAGTGAAGCAGCAGCTCTAGAGGGGAGATGTGGGCATTAGACCCCAGCACTCACAGAC
    TGGGGCTGGCCCTGTTGGGGGGAGTCTGGGCACTGGTTGATCTGGTGCCTTTGCTCAGAAACCAGACCGT
    GATGACTGGGAGAATGGGCTGAATGCCATGGAGTGCGCCCTTCACTTGGAGAAGAGTGTGAATCAGTCAC
    TCCTGGAGCTGCACAAGCTGGCCACTGACAAGAATGACCCTCATGTGAGTACAGGGATTGGGCTGGCAGC
    AGAGGGAGGTGGGGTTCACGAGGCACCGGGAGCCAACCATCTACCTCTTGTCTCCTAGCTGTGCGACTTC
    ATCGAGACTCACTACCTGGATGAGCAGGTGAAGGCCATCAAAGAGCTGGGCGACCACGTGACCAACCTGC
    GCAAGATGGGTGCCCCTGAGTCTGGCATGGCCGAGTATCTCTTTGACAAGCACACCCTGGGGGACAGTGA
    CGAGAGCTAACGGTGCTTGGGCAGTGTGTGCATGCTGCGGCTACCTTTACCTTTTCTATAAATTGTACCA
    GAACATCTAAAGTTCTTTAATTCCTTCAAATAAAGAGATTTGGTACCC
  • Protein Sequence : Show Sequence
    >gi|290491181|ref|NP_001166318.1| ferritin heavy chain [Cavia porcellus]
    MTTASPSQVRQNYHQDAEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKL
    MKLQNQRGGRIFLQDIKKPDRDDWENGLNAMECALHLEKSVNQSLLELHKLATDKNDPHLCDFIETHYLD
    EQVKAIKELGDHVTNLRKMGAPESGMAEYLFDKHTLGDSDES
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
11. Ftl
  • Gene Name : Ftl
  • Sequence Strain (Species/Organism) : Cavia porcellus
  • NCBI Gene ID : 100379558
  • NCBI Protein GI : 290491214
  • Protein Accession : NP_001166329
  • Taxonomy ID : 10141
  • Chromosome No : Un
  • Gene Starting Position : 8357696
  • Gene Ending Position : 8359000
  • Gene Strand (Orientation) : -
  • Protein Name : ferritin light chain 1
  • Protein pI : 5.89
  • Protein Weight : 19110.14
  • Protein Length : 175
  • DNA Sequence : Show Sequence
    >gi|347623385:8357696-8359000 Cavia porcellus strain inbred line 2N unplaced genomic scaffold, Cavpor3.0 supercont2_70, whole genome shotgun sequence
    TGCTGTGAAAGGCTTTATTTTTCTTTTGGTCCAAGATTTGGGAGGGGACTCCAAAGAGGTGAAAAAACTG
    CCTAGTGACTGGAGGGAGAGGGTCCAGCCAAAGCCCTGATGCCAGTGATGCTGGAGCCCCTCAGATACTG
    CCAGGCTCTGGGGCCTCCTAGTCGTGCTTAAGTGTGAGCCTTTCAAAGAGGTACTCGCCCAGCCCAGCCT
    GGGGACCATCCAGCCTGCGGAGGTTAGTGAGGTGGTCACCTATCTTCTTGATGAGCTTCACTTCCTCATC
    TAGGAAGTGGTTCTCCAGAAAATCACAAACCTGAAAAGCAAAAGAAGTCAGAAATCCAATTCTGGGCCAA
    GAGGGTGGAGCAAAGCCGTTTCTAACAATGGGACATGCAGGAGGCAGGCTCAGAAGTAAATGTGCCTACG
    GCTCCTGGGACAGTCACTTACATGAGGGTCTGTCTTGGCAGAGCCCAAGGCATGGAGATCCAGAAGAGCT
    TGGTTCAGGCTCTTCTCCAGGGTCAGAGCCGCTTCCATAGCATCAAGGGTCTTACCCCACTCATCCTCAG
    AAGGCTTCTACACAGAAAACAAAAGGCACAGAAACGGCCACGTTATCGTACACACTCACAGAGAGCTAAA
    ACTACAAATCTCAGTTTGAGTGATCCTTTCCTGCAGTAACTTGCACAGCCTACACCTGATTTCCCAGAAA
    TCCCAGCGACAACTGATCAGCTAAGGCCCAGGAGGAGCCCCATGGCAAGGACTGAAGTCGCTACTTCAGT
    CCTTGTTTACCTGCACGTCCTGGAAGAGCGCGCGGCCGCCGCGCTGGTTTTGCATTTTCAGGAGGCGCTC
    GGCACCCTCACGTTTCTCCTTGGCCAGCTCACGGAAGAAATGGCCCACGCCCGCCAAAGCCACATCGTCG
    CGGTCGAAATAGTAGCCCTGCGGGAAGAGATGGCGGGAAGTATGTGGAGAGTTGAGGCCAGGCAAGCGGC
    CTCCTCCCTCCGCCTGCCCCGCAAAACAAAGACGGCCTGCGCATGCGCACAGCCAACAAGTGCGCAGTTA
    CGAGGAAACAGGGCCTAGGGTGTTTGGGGGACCCTTACCAGAGAGAGGTAGGTGTAGGAGGCCTGCAAGT
    GCAGGTTCACCAAGCGGTTGACGGCAGCCTCCACCTCGGTGGAATAATTCTGACGAATCTGAGAGGTCAT
    TGCTGATCAGCGGGAAGGAGCTACCCACAAAAGAACGGTGTAGGCTGGCCCTGGAAGGAGCAGATGGTCG
    AGAAGATGGTCCCAAAATTTGTACCTGGAGAGTGGATGGGAGATT
  • Protein Sequence : Show Sequence
    >gi|290491214|ref|NP_001166329.1| ferritin light chain 1 [Cavia porcellus]
    MTSQIRQNYSTEVEAAVNRLVNLHLQASYTYLSLGYYFDRDDVALAGVGHFFRELAKEKREGAERLLKTQ
    NQRGGRALFQDVQKPSEDEWGKTLDAMEAALTLEKSLNQALLDLHALGSAKTDSHVCDFLENHFLDEEVK
    LIKKIGDHLTNLRRLDGPQAGLGEYLFERLTLKHD
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
12. GPR182
  • Gene Name : GPR182
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 11318
  • NCBI Protein GI : 6005705
  • Genbank Accession : AC026120
  • Protein Accession : NP_009195
  • Taxonomy ID : 9606
  • Chromosome No : 12
  • Gene Starting Position : 54427153
  • Gene Ending Position : 54444913
  • Gene Strand (Orientation) : +
  • Protein Name : G protein-coupled receptor 182
  • Protein pI : 7.55
  • Protein Weight : 41850.38
  • Protein Length : 404
  • Protein Note : Also known as AMR; 7TMR; ADMR; AM-R; G10D; gamrh; hrhAMR; MGC34399
  • DNA Sequence : Show Sequence
    >gi|157704453:54427153-54444913 Homo sapiens chromosome 12, alternate assembly HuRef, whole genome shotgun sequence
    CCAGCCTCCTCACAGCTCCCCATAGCCTGGACCTGCCGGCCCTCCCTCCAGGACCGAGGGGCTCCCAAGG
    GAAACTCAGGTGAGTGTCGCCCTCTCCCAGGGAAGATGGGAATCAGGGCTTGGAGACAACTGGCTATCCT
    GCATGATCCACAGGTTTTCCTAGGGATGAATCAGATTGTGTGGTGTGCGTGTGTGTGTGTGTGTTCCACA
    CTCACGGTCCAAATAACAAGACAGAAAAAGAGCAATAAAGATCTGTGCAAAAGGGCTGAGGAATGAAGTG
    CCAGGAGAGAGGTGGAGGTTGGAGACCACCTAGGAATGGTGGGTGTGGGGGGATTGAGGGGGTTGTTGAG
    GGGGAACTGGCTGGGGGTGGGGAGAAATGTTGGTGGACACCAACAGTCCTGGCCTCTTCCAGCTGAAACC
    CCTTAAGAAAGTATCTCTGCTCTTGGGTCAGAGCTAGCAAGTGAGTTGTGTATTATTTATTCACAGTTTG
    AAAGCTAAACTTCCCTTTCTTGCCTTAGCCCCAGAATCTTTCCCCAAAGCCCCCGACTCCCTCTGGCCTC
    TACCTTGAGGACACAAGGCTCTCGAGGTCTGCTTTCCCTTCCAACCCTCCCTTCATCCCAATAGGCGTGT
    GCTGGTCCCAATGTCAGTGAAACCCAGCTGGGGGCCTGGCCCCTCGGAGGGGGTCACCGCAGTGCCTACC
    AGTGACCTTGGAGAGATCCACAACTGGACCGAGCTGCTTGACCTCTTCAACCACACTTTGTCTGAGTGCC
    ACGTGGAGCTCAGCCAGAGCACCAAGCGCGTGGTCCTCTTTGCCCTCTACCTGGCCATGTTTGTGGTTGG
    GCTGGTGGAGAACCTCCTGGTGATATGCGTCAACTGGCGCGGCTCAGGCCGGGCAGGGCTGATGAACCTC
    TACATCCTCAACATGGCCATCGCGGACCTGGGCATTGTCCTGTCTCTGCCCGTGTGGATGCTGGAGGTCA
    CGCTGGACTACACCTGGCTCTGGGGCAGCTTCTCCTGCCGCTTCACTCACTACTTCTACTTTGTCAACAT
    GTATAGCAGCATCTTCTTCCTGGTGTGCCTCAGTGTCGACCGCTATGTCACCCTCACCAGCGCCTCCCCC
    TCCTGGCAGCGTTACCAGCACCGAGTGCGGCGGGCCATGTGTGCAGGCATCTGGGTCCTCTCGGCCATCA
    TCCCGCTGCCTGAGGTGGTCCACATCCAGCTGGTGGAGGGCCCTGAGCCCATGTGCCTCTTCATGGCACC
    TTTTGAAACGTACAGCACCTGGGCCCTGGCGGTGGCCCTGTCCACCACCATCCTGGGCTTCCTGCTGCCC
    TTCCCTCTCATCACAGTCTTCAATGTGCTGACAGCCTGCCGGCTGCGGCAGCCAGGACAACCCAAGAGCC
    GGCGCCACTGCCTGCTGCTGTGCGCCTACGTGGCCGTCTTTGTCATGTGCTGGCTGCCCTATCATGTGAC
    CCTGCTGCTGCTCACACTGCATGGGACCCACATCTCCCTCCACTGCCACCTGGTCCACCTGCTCTACTTC
    TTCTATGATGTCATTGACTGCTTCTCCATGCTGCACTGTGTCATCAACCCCATCCTTTACAACTTTCTCA
    GCCCACACTTCCGGGGCCGGCTCCTGAATGCTGTAGTCCATTACCTTCCTAAGGACCAGACCAAGGCGGG
    CACATGCGCCTCCTCTTCCTCCTGTTCCACCCAGCATTCCATCATCATCACCAAGGGTGATAGCCAGCCT
    GCTGCAGCAGCCCCCCACCCTGAGCCAAGCCTGAGCTTTCAGGCACACCATTTGCTTCCAAATACTTCCC
    CCATCTCTCCCACTCAGCCTCTTACACCCAGCTGAGGTAGAGGCCAGACTCCTCCAACAGTGAAGGAAAA
    GGCACAGGTGAGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGATAGCCATGGACTCTGGATCTTTA
    AAAATCTGCACTTCCAGCTGGGATTGGGAAGGGAGCTGAGGCTAAAGAGGTGCTCGGCAGAGGAAACTAG
    ACACATTCATTTCATAAATCACTCTGGCTGGAAACAGTGAACTCTGCAGGAACCTCACAAAACTCCTTTT
    GCGTTTGTGAGCTAGATGATCTTCCAGCTCACTATGCAGGTTATGGGGACAAGAGGCCAGAGGCCAAGGA
    ACCAGGCGGCCCCAGCAGGACGTTCCCTAGCTGTGCATCCTATGCACAGCCATGATAAGTGAAGGCGGAT
    GGGGATGGCAGGGGTGGGGGCGAAGCCAAGGTCTGAGACCTGCACCCTGCAGCTGGGTCAGCAGGATGGT
    ATCTCCACCCGCGGCCATCCAGGAAACACAGCTCCATTTCCTTGCTGACACCCTCCCCTCCCCGTTGTCC
    ATCCTGCTCCCTCCCCACAAGCAAGAGGAGTTGTCCCAGAGTCAGCTCCTCATAGCTGACCTCTTACCAG
    CTTCGGATCTAGGAAACTTCCAGACTTCTAGGGAGACACAGAGCTACCAGAAAGCCCAACCCACCCCCAT
    CTCTTTCTCTCCAGATCATAGGAAAGTATCAAGGGATGTCATTCCGGGAGCAGCCCTTGGGACAGCCAGC
    AGCAGGTGCTGGCAGATGCTCTGTCCTTCACCGTCTGTCCCCGGCACCAGATGGGGAAGGCCACAGTGGA
    AACTTCTCAATTCCGTCACAGGGCAGAGCTCTGACACTGTTATAAGTCAGTTCTCCCTGGCCATGTCTTT
    TCCTAATTTTCCTGTCCCACAACTTAGGTTCCTTAACACTTGTTCTACAGATGAAACAAAAAAGTCCTCT
    GTTAACAAGTAGACCTTCTGGAAAAGGATCACTTATGTTTGGGAGACTAGGACCCTGTCCATGAAGAACT
    CTGAAGTAAAGGCCTAAGAACAGGATCTAGTTTCCGCCTTCCAATTCCACGTCCTTTGCCTCTTAATCCT
    GCCCGTGGTAAGGGGACTGGATGGAATCACTGATAGCCTCTTCACCATCTCCTCTGTCCTGCTTAAGTGC
    TCCATGTGTCTGGAATGTTTAACTTGGAGTAACTACTCACTGACTTGGAGACCACCTGTTCTACTTTGAG
    AAATCAGAACTGTTCTGTGGATGCACTGACAGCGTGCTCCAACTGCATATGCACCCAGGTTTGTCAGGAG
    CATGGAAGGAAACCTGCATCTCCCTGTTTACTGGGCCCAAACCTGAAAGGGTCTTAAGCTGCAGACAGCT
    TCTCAGTAGCACAGAGCTGAAGGCTTTTTGAACCAGGAGTCTGGGTTGTGGCTTGGGTCGCAGTAAGGGC
    TTAGCCTATCAATGCAGAATTCACATTGAAAAAGACTTTCCTCTCTTGTCCCAACACAAAAAAGCAAGCC
    AAGATCTGATGCCCATATCACTGCCTCCTCTGACTTGGGCTACAGGAGTTTCCTGAAGGAAACCAAATTT
    TACATCTCTGTCACATTCCAGATCACCTTAGAGGCCACGCTCCCAAGATTAGTTATCACCCTGGCAGTAT
    GAATACTTCCCTAAGGCCTCCCATCCATGGAGGGGAAGAGTGGGAACCAGCTGTTACACTCAGCATCTAC
    TGAGCACTGATGGGAGCCCTGCCTGGGCCATGTGCTGTGGGGCCAAATGGACATCACTCCCTAACTTCTT
    GAGGTCTGTCTCCTCTGTGGATTCACGCATGTATCAACCTCATTAAACTATGCCAAACTTTTGCCTCCTC
    CCTGTTTCAATCTGCCTCGCTCCACTCAAAGACACACCATGTCCCCGCAAATCAAATGGCATTGGCAAGA
    CAAACTGTAGCCAAATCTCTCACTTAGTAACGTTAGTCCAACTAGACACGGGCAGGGACCCACCACTCTC
    TCTGGGCTGACGGAGGATCCTCTCAGGGATACCCCCTGAAAAGTTTGAGTCTTTGACTTAATTTCCCACT
    AAAAACAGGTCAACAAGTTAATCCTGGGGAATTCCAGCTACAGGAAAGGAAAATGTGCTACCTGAAAGAA
    TTCTGGCATCTGCTGGGCAGAAATGTGCTGCTGAAAGGGTGATGAGCCCAGATATTCTGAACTCCAGGTA
    TGAGCATATTCATAGTAGGTAGGAAGAGAACTCTCATTTCAAATTTCTTTTTTTATTCTAAATAAAAACC
    ACACTTTGTGCTGAACAATGGAATATAAAAGAATCAGATGTACAATGATTTTAGACATCTACTATTTGGG
    GAAAAAAGTTTACAAAATATACAAAACTTTACAGCAATAGATAGTAAACACTTAAATATTAGGAGGTCAG
    TACTTATTAATTTAATGGAAGGAGTTAGACGTCAACAACTCTTCTCTGTTTCATAAGAGACTTTAGCTAT
    TCAAGGTGGGGGCGGCGAGTGGATTGGACAGGGGAAGGGTTTTAAAGAAACATCAAAATCGAGTTTCATT
    TTCATTCTGATGTTAAGCTTATGATGCATAACCACATTGAAACTAATGCTGTGCACCCTGCCTCCTGACT
    CATTTGGTGCAGGATTTAGGGAAAAGAGTCCCAGATGGGTGCAGACCACCCCATAGAGCCCACTCCCATA
    CCAATAAATACAGTGTCTATCTAGAATTTGCTTTGGAAGTTAAGCAGTCTTGGGCTTGGGGAAAGGGGCA
    CAGAAGGAGGCCAGAGGAAGAAGAAAATTCAACACTAGCAAAAACCTGTCTAGGAAAAAGATATTTAAGC
    CCAAAGGTTTGGCCTGGAGACAAACAGGTACTCACAGATCAGAAAAGCAGAATATAAAAAAAAACTTTTA
    CCCATCTCCTGGTTCTTAGGGGTCACCTGGGCCTGACCCTTATCCCACCTCCTTTCTTCCTAACTAGCCA
    TGGACTACGGGAACTCCATGGAGTCATAAAATTTAGGACCAAATACTAAGGTTCAGCAGAGCTTCCTAGC
    GGGTGCTGTGGCATCAGTGAGTCACCCAGTGGGTCACTGGAGTGCCAGGATACTAATCTCTGCCTCTGGC
    CACATGGAGCCTCTGCTGTTTAACCCCGGGTGCCACACAAGTATTACCATTTTCCCCTCGGAAAACAAGT
    TGGGAATGGCCTCATATTTGGTTTAATGCTCTATGACCACCACCTTGAAATTTTTAATAATTTATGAATA
    AGAGGGCTCCACAAATGATGTAGCTGGTCCTGAGTACATACCAAGAAAATGGGAGAAGAGGATGTTCAAG
    GCTTAAACAGAACTTCAGAGTAACACAGAAGAGAGTAGTAAACTAGGTTCCAGGAGAGAGGGAGCAACAA
    AATCAAATGAACAGAAAGTCAAAACAATTAAAAATGCAAAATAAAATGAATACCTTCTACCAGGTATCCC
    AGGGTGCCCTCTGCCACAATATAGTGTTTCAAGCAAAACCATTCAGAAGAGGGTGAGCTGGAGGCAGAGG
    AAGCAGACATGACTTTGGTGGGGTGGGGGGAAACGGGAAGTTCAGGTGTGAATACAGGGGTCACCAACAG
    GGAGAGGGTTTGGTCAACAAATCTCATGAGCTACTGATCCTATGACGTATCTGACTGGGACCAACTGAGC
    CACCCTGATCAATCTGATCAGCCCCCGGGGGATCAGGGGATGCAGTTTTCTGTTTCGTGTTTCAAGGGCC
    AAGTTTCAAGGATCTGCTTTTTGGTTTACTGGCAAAAAGGGAAAACTTCACAGTTTCAGGTTAAGTACAC
    AGACCACCCAGCATCCATGATGCCCTCTGTTAAGGAAAAAGGTTTCTCCAGCACATTTATCTGCTCTCAG
    CCACTCGGCTGGTTCCTGTAATTACCACCTCTCTCACACCAGCATTTCAAAAATCAGTCCTCACCAACAG
    TCTGGGAGGGATTGCTCATGCTTGAAAATGTAGCAGACACTTGACTGTAGTCTGTTACACATTTCCTTCT
    TAAATTCTAATGGTCAGTGTGAGTAAGAAAGGGAGAAGCCAGTCAGCAATGAAGAGTCTCAACACTGCTT
    TCCTGATGAGGATGTCCTCAGAGGACACTGAACAAGGTGGGTTTAGATGGTCAAAGGTGGACCTTTAAAC
    TGGCCACTGTACAAGTCCCTTCATCTAATGGTCACCCCACCAGAGAGCCAGGCACTCCAACTCTGAGGCT
    TTATCAGTTCAGGGCTGGGGAGAAGCAGCAGGTCCAACAAGGATGGAGCTTTGGTTTGTCTGACATCTCA
    ATATAAATAAAGACAGGAGGCTTCCAAACTGTGCAAATAGGTCATTCATGTCACTTGTTCCCCTCAACCC
    TGTCCCCAATCTACTCCATCACAGGAAGGAAGGGGGATGAAAGGCCTGGAATTTAACTGTCCCGAACTTT
    ATAGAACATACACCCATGGCCTAAAGTGTTCCCTGGGTTTATTATGCTAGAGACAATCTGGAGAAGAAGA
    ATTTGCTTAAGGGCTTGACATGTCTTCCCTTAGTTATACCCCTGGTTCTCCACATCTCTGGTCAAGGCTG
    TTCTCTGGAATATGGCTACTTGCTGGGGAGAAACTCATTTTCCTAAAGTCTCTTATACAAAAGAGATGCT
    GGGTAATGTGTATACAAAAACAAAAATAAATAAATATTAATGTCCAAAAAGGCTTTGTGAGTTAATGAAA
    ACCACTTGCTTGTGAAACAGCCCGGGGTGTTGCTGAATCCCACCAGGGGCAGTGCCAGGGCTGAGGCCAG
    GGACTCCCAGAGCTCACCTGAGCAGGGTGAGGTAGTATGAGCTACTGAGGTGGCACCTCACAAGTATGCA
    AGATCGAGGGCCCTATCCCTCAAGACTTCTTTGGGCAAAGCCCCTTAGAAAACCAAGTAAGAGTGGAGCT
    AACCAGCTGCGTGGGCTGTGCCGGCACACTCTGCAGCCACCCACGGAGGGCCTGAACCAGCTGCCATCCA
    ATAGCGCCCCCTGGAGGTGATGGCAACGACAGATCAGGATAAATTCCAGCAGGTCAAAGGTGAGCTGACA
    AAGCTCCGGCACTGTTAGCACAAAGTAAACAGGTTACCCGACTAGGACAGACAGTCAGAACGCACACCCT
    GCTACACACCACCAGTTACTCAGGTCCCTTTAATCCTGAAGGGCTCGCGGTCTGCCACTCCACCACTTCA
    TGGAGTCAGCTCATCTACACCACGGGCCAAACACTTCTGGACGGGAGACCAGATATAAATTAAGGCAAGG
    CTGCTGCTGTCAGAGACATCCCCACCTCTCCACCAAGACACTCATGGCCAACAGAAGTGGCCAAAACAGA
    TGACTATATCAAAGTGGTTCCCTATCCTCTCTGCCCCACAGAAGGCCGGACGTCCCGGGCCATCACTCCA
    TAAAATCCTGTTTCTTTGGAAATGAGGAGAAAAGAGAAACCTCTCCTGTCCATCCAGGCTGCCACTTCTG
    GTGGCACTGCAGGACACCCCTTCTGGCTGGCAGGGGTTCATCCCAAAGCTTTCTGACCTAAATAGAAAGC
    TCTGTGCCTAGTATGGATCTAGCTGCCAAAAGATTTTCACTGTTCAATCAGCAGATTAGGGCTGTAGATA
    AGCAGACACCAACATCCCTGATGTCTTGTCCCTGCTGAGGGCTGGAACCTGAGGGGACTGCCCTGCTGAC
    CTGTCTTTTCTGAATGATCACATTCCCAAAATCTTTCTCATTGCTGGTCATCTCAGTTGGAGGCCTGCAG
    TAACACCCACAGTGAAAACCTCCCTTTACCTCCTCTGTTAAGTGAGGCTGTTGGTTGGATTCTTAGCAGC
    ATAAAACCTATCAGTAAACTAAAACTAGAGAGGCTGGGGAAGGGGGTCTAGTGGACACCAACTGCTTGAC
    TAGATCCCAAAACCAGCCTCAAAAGAATGAAAAACATAGCCCTGCATGGGCAAGAACAGGTATGGAGGTA
    AATGTGATAGGGCTGTTGCTTCTCAACAACAGTAACAGCTTATGTGCTATTTCTTCTTCTTTTCTTCTTT
    AAACACAACACAGAACAGAATTCCTACCAAGGCAAAATTATAACTTCAGCTGGGGAGGGACCAACACCTC
    TTAGATATCAGCATCCTGGCTGCTGCTTGGGAGCTCCCCGTGGCTCTGCCGGGACCCAGTCAACTGTCCG
    GGTTCTTATCCGCCTCTTTGGAATGGATGATGTACATGAAGGTCTGCTCGATGGTGAAGTCAGGGGGGAG
    GCTGCCTTTGTGCACACCAACATGTTTGCTCATGAGGTTAAGGGTGGAACTGTAGTGCCCACAGACTGTG
    CAGCGGTACATGAGGGCCCCCGAGTGTGTCTTTAGGTGGCACTTGATGGTCGAGCGGCCTCGAAAGAACT
    TGTGGCACACCTTACATTGGTATGGCTTCTCCCCCGTGTGGATCCGCCGGTGGTAGTTGAATTCGCTTGT
    GTGGGCAAATCTTTTGCCACAGACCTTGCAGCTATACTTGCTGTTCCCAGGTTGGCCCTGCAGCGCCAGC
    TCTTCACCCAGAAACTCCTCTGCTGGCAGCTTCCGCTTCTGGAGAGCTGGGTGCTGCAGCCCAAAACCAG
    GCCGTGCATCAAGGCCACTGTTGCATTTGTGCTGGCTGACGTGGTAGCGATAGGCAGCCTCTGACTTGAA
    GCTCTGGCTGCACAAGCGGCAGTGGAAGAGGGCGTCTTCCAGACTTTGGTGCACAGCCATGTGCTTCTCT
    AGAAGATGCCAGTCCACAAAGCTGTTCGCACAGACAGAACAGGAGAAGACTGAGATGCCGAGATGGGACA
    GCGTGTGCCACATGAGGCTGCAGAGGTTAAGGTGACGCTGGTCGCAGACACTGCAGGCCTGGCCCTTCAA
    GTTTAGATGGTCAAGGATGTGGCCCCGGACCACATGGAAATCTTTGGCCAATACTTTCCCACAGGCAGCG
    CATTTCAGCTCTGGGGAGGCTGCCCCTGAAAAGAGACCCAGCTTCCCTGGCAGGGGATCGTTGGGGTGGA
    CAATGATGTTGTTCTCAAATATTCCATCCCGTCGGTGAAGGGTCAGCTGCCACTGGGTAAAGAACTTAGT
    TTCACACATGTCGCAGGAGAAAAGGAAAATACCAATGTGGGACAGGACATGAGTTACCCGGGAGTTTCGG
    TCCTGGAAGTGGGTCTCGCAGACCTTGCAGTTGCCCGTCAGCAGGTCCACATGGTCCCGAGCATGCTGCC
    GGATCAGTTGAATGTTGGGCTCTAGAACTTTCTTGCACACCTGGCAGGGCATGGCCTTATTCTCCCGATT
    GTCATTCTCTCCAAAAGCCAACTCATCCTCACTGTCATCACTCAGCTCAATCACCTCCTCAGTTGTGCCT
    ATATCCTCAGCAGGGTCTTCAAACTGCAAGTCATCATCCCCCAGGTCCTCGAGCTGGAGCTCATCCATCT
    GCCCAAAGCCAGGATCTTTGGAGTGCTCACTATTGCTCAGACAGGAGTTGGTCCCAGTGGTAATGTCTAC
    TGCATTGTCAGGGGTGAGGAAGCTGTTTTTACTGAAGTCTCCATTGCTTTGAACTTTGTATGGCTGGCAG
    GAGCTCGTGCTGGTCTGGATGCCCGTGCTGACAGTGCCTAGGAGTGGCTGGGTCATCATGCTAGGAATGG
    ACGTGAAGGGAGCAGGGGTGTCATGGTCTTCTGTCTTTGGCGGTGGTGGGGGCGGTTGCGGCAGATGCAG
    GCTATGGTTAGCGTCACTGGCAACATTCTTCTCTTCCTCTTTATAGCTCCCTCCAGCATCACTGGGCAAC
    ACATAGTTTCTATCAGCACCAAGGTAGTCCCCACCACCTCGGAGTTCTCCAAGGGGATGGGCAGGTTCTG
    CCGAAGGACAACTCAGGGTACCAGAGTGGCTCTCACTGGTGCTGGTCAGGGGCTTGGCCCTGGCAGTGCT
    CTCCAGATCAGGAAAGGTAGAGTGACAGGCCTGGAGGAGGTCCTCCATACCCAGACGCTCAGCTACCTCG
    TAGATGACCCCAACATTGATCAGGTCTGTGAAGAGTTCTGAAGTGTAGACAAAGCTCAGAACCTTCTCAA
    AGTTGGCAGGGGTGATGAAGTCCACCACATAGGTCCTGGCAGCATCAAGCCCAGTATTCAGGAAGAGGTT
    CTGGAAGTAGCCAGCTGCACAGGCCAGCACAGCCTTGTGGGCCGGGAAGGAGCGGCTCCCCACCACAATG
    GTGACGTCGCACATGGTCTCTGAGAGCCGGCACTTGTTGAGTTCCTTCAGCAGGTTGTTGGGGTGGTTGG
    TGCTTTGCAGTTTGATCCTCATGCCCATCTTAGCAGCTCCTATGAAATTACCTCCTTATCAGCACAGTTA
    ATCTGTGGATAGCAAGAGAAAAAGATGGATGGCCAAACACATCCGTGCAAAAGAGGGGCTATGCAGGAGA
    AAATACCATCACTCTGAATGCTTCCAAGCCCCAGGTAGGGATCAGGAGTAAACTGGCATGGAGAGTTCCT
    CCATTATGACAAGGCAATGACATCTCCCCCTGTATATACTCAAAGAGTTATGACAAAAACCAAAACGACT
    GATGACAATCTGATAGCTTGGTTTTAAGCTGGTGCCTAGAGACGAACCAGAGGCATTCCCTTGGACACAA
    TCTTATACTTCATTACCAGAGCCACTGGATCAAATTTTTCTCCTATTAATTTCCTCCCAAATACAAGCTT
    TCCTAATTCATTGAGCATATTCTTTTCCCTCATGATGCCAAATACCATGCACATGCTTGAACACAACTAT
    CAAAGCTCCACTAGCTACTGAGGAACATATAAGGTGGTTCCGTTTTTTAGCTTTGTCATTGCTGTGTCTT
    CAACCTCTCAATAATCTCCAAATAAATCTCACGTTCTTGAATCCCCATCTCTCTCTGCCTATACTACATA
    CCCGCTCTCTGGCCTTTCGATAACCCAGAGCCCCTCCAGATATTGCTTTATCACAGTCCCTGTATTCTGT
    GAAGTTAAAACACTCAGAATGCACAGTAAGAAAAGTAGAACACAACTGTGGTAGTATTTAATATTACTTT
    AATATGTTGTATTCTTTTTCGCCACCCTTTTCGGGGTTTATATTTTGTTGTCCCTGCACTAACAGATTTA
    CTTGATGCCGCAAGAAAGACTTCTAATTATACAGGTAAGACTCCTAGAGGCTTGTTCACTACCACTTTCT
    GCCCTTTCTGACTCCTGCACGACTAAGGAAGAAGAGCTACTTGCATCCCTGTTCAGTCTCACAACTCTGC
    CCAACACAGCCCCTTTCTCCATCTCTCCAGATTCTAACAACTCCCAACTTTTTCTGGGGCCCAGGAAGCG
    TCTAAGTATTGTGGGGTACCGGCCAAGCATCTCCCGGGCCCTGGTGGCCACCCCTGAACGATGGGCGGGT
    CGGAGCACTGGGCAGCCAGAGGAGAGGGTGGGAGAGGGGGAAGCTGGGAACTTCCAAGGACACCCCAACA
    ATCCCCGGGCGCCGCGGTCTCCCCTCCGGAGTCGCATCTGGGAGGGAAGGGCGCTGCATCGGCTGGGGCG
    CTCCCTCTGGAGGCAACGAGCGCCTCAGTCCCTCCACAAACAGCGCGGGGCTCACCCGGGTGAGCCTGTT
    GGGGGCATGGGGCTGAATAAGGGGCCAAGCGAGGGGCGGCGGGGCCGGCCGGGGTCGGCGCGCGGGCGGG
    GAGGATGGGTCCGGCCGAAGGGAGCGGGCAAGAGGGACCCCAGCAAGAGCGAGATAAATGCCGGGCCGAA
    GGTCGGGCTTCGGCCAGGGGCAGCCCTCGGCCTGGCCGCGCTTACCCGGCTCTGCGGCGCCCCGAGCACC
    ATCGCCGCCGCCGCTTCACACAAAGGCCCCGGCCCGCCGTGCCCGGCCCGACGAGGAGGCGGCGCCGCCG
    GCCGCGGCCAGACTCTCCATCCGCCGCGCGGNNNNNNNNNNNNNNNNNNNNAAAACCCTATCCAAACTCT
    CTTCACCTCCCCTGTTCTACGCTCACAGTTCCAAAAGCTTCTCCTCAGAACATCTACTATGGGCATTTTA
    CAAAATGTTGTAAGATTGTTTGATTACTATATATCTCCTCCACTAGGCTGTGGGCTTCCTGAAGGCAGGG
    ATCTGTGGCTTTGCACACGGCCGCAGGTCCACACTGACCCTTACCACACTTCCTGGCACAATAAGTGCTT
    AATACATTAAGCCTGCTTGAATGAATGAAGGAGCACTTGAGGGCTAGACCGCTACTACTGGACCCTTCTG
    ATAATCCACTGCTGTCCCTCCTTCCATCTAGGTGGCCAAATGAAGAAAGTCACCGGATCTCACTGTACTT
    TGGCACCCCAGGCCTGCTTCCTGAAGCCTGCCCTCCCCAACTCTGTGTAGAACTCGATCACTCTTCCTCC
    CCACCCTTCTCCCAAATCATCTTTTCAAATGCTTCTGCCCAAGAATTGTCATCAAACGAAAACCACCTAC
    ACTGCCTGCCCCACCCACCACCACCAAAGACATTAGAAGTCTTCGTCATGTCGCCCTCCCTCCCTCAGTA
    CAGAGGAAGCTCTTAGGCTCCAAGACCCTTCAGTTTCATCCTCTCTCCCATCCCATCCCAGTCACTGCCT
    GAAAATAGCTTGCAGCCAAGGACAAACTCCCAGGACAGGCGCATGGTAGGATTCAGTAATTCCTTGATGG
    ATAAGTGGAAAGGAAGGGACACACTGAATGTTGCCAACAGATCTAAGCCAGTTTGGCCATTCAGCAATTG
    TCCATTCTCTTGATGAATTTGGGAGATTGTGTGTCCTCCTTTCCACAACCTCATTAACTGGTGGCAGACT
    ATACTGAAAGCAAGCACTATACCAAATGCTGCCAAACATGTAACATTTGTTAGCCAGGAGATACAAAAAC
    AGCCATAGAACTGTTCTTATAGTTTGGGGATGAGGCATCTCACTTTACCATCTAGTGAAAGGGTCACTTA
    GAGGTTGACACAATGTTTCAGTGGCAGGCCCAGCTCTCACAGTTGTGCAAGGTGACTGAGTCTCCCTAAA
    GTGGAATATCACACAGCCAGCAGGTCCAGACCTCCAGCATGACTCTGGAGACAGAGGGCTGCTGGGCTTG
    CAAGGGCCTGGGCCTATACAGTCTTTCTAACCAACTGCCCCAGCCATCTCTGGAGGGTTGACCCCAATAA
    ACTTCACATGAAAACAAATCATCCAAAAGACGCAGGTGAAAGTATATACCACTTATACTGAAGTCTTTTT
    AAAGTAAATCACCATATAGTCACAATAACAAACATATACAAAGTTAAATGGAGAAGAAAGGGCCAGGAAT
    TTAGTCAAGGTCTTGTGGACCAGATCTCTTTAGGCATCAACTGTTTTACAACAGGAAAGGGAGGGTCAGG
    ATCCCTTCACACATTCTTCAAGTACAAACACAGGAATCTAACTTAACATTTGTCTTCATTTTTTTCTGGA
    GTCCCCAGTACTGCAATTTTTGTCAGTCCCACTAGAGAGCAATCTTTCTGCACTTAATGTTCCTACTTGC
    AAAGCAATTGTCTCATCTCAGCCTATAAGCCTAACTCCCACCAAGTCTGAGCAGAACCAACTCTTGTAGC
    TTTCATGACCCAGAGAAAGCCCCTAGACTCTGCAAAAGTGTCCCCAGTTTTAAGCCCTATAGATAGATAA
    TTTTGAAAAGTCAAAGAGATGACTCACTGGAAAAAGGGTCTAGGCTCTGGGGTGGGGGCATTCTTTGACC
    TCTGGCTATAAAGCTGACATAAGTAATACTAGGGTGAGTATTCCCTCCTTTCCCCGCCCCCCACTATACA
    GCTGTCATTCCAAAGTGGCCCCAAGTCAGGCTGTAGGAAGATAGAATTTTCACTAGAATGAAAGGACTGC
    AGGAAGTGGCCTCTGGCTCTGTGGCCCTGTCCTTACACTGCCTTGGGAAATCTATGTGTTGATGTTTTAT
    CAGTGCAATTCAGGGGCATAACAAAGGCCCCTGTACCACGCTAAGATAAGGAGCCTGAGGATTTCACTGG
    CTGTTCCATTAGGGTACAGAACCCCCCTTAGGATGGCGGGTGATAGTTGACATCATAGATTAGTAAAAGA
    CATCCATACCCATGCATACCACATGTAAGTGCCTTACACATTCTGGGGACTGTTAAGTCAGAGCTGCAGG
    AGCTGGTAACAGGCACTTTAGAGAGGAGTGGGAGATAAATGTTTTCCCTTTATAATAACTAAGGCAGCCA
    GTTAACGGTGAAGATATCTGCTCCTCTCTTTCTTGGCCTCGGTAGTAACTTCCCTGTCCACATCCCATCC
    TAGCTGGATCAAGTTCTGTTTGCTTGTCCACAGCCCTGCTAAACTGTAAACTCTGTGTATGAGTCATCAT
    TTCATCCCCAGGATCTGTCACAGAACCCAGGCTCAGCATTTGGAGACAAAAAAATTGTATAGGGTCATAG
    ATTTGTCCTCCTCTGTGGCTGGGTCTCCGGACAGCCTTGATCCCCACCTCCTTCTCTAATTAAGTAGGGT
    CAGATTCAGGAATGAATATCTCCCAGACCCATCCTAGTAATACAAGTCATTCTGAATATTTTTTTTCTTT
    TTCTTTTTCTTTTTTTATTGTTTTTTTTGTTTGTTTGTTTTGTTTTGAGACGGAGTTTTTTGCTCTTGTT
    GCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAACCCCTGCCTCCTGGGTTCAAACGATTCT
    CCTGCCTCAGCCTCCCAAGTAGCTGGAATTACAGGCATGCGCCACCATGCCTGGCTAATTTTTGTATTTT
    TAGTAGAGAAGGGGTTTCACCATGTTGGTCAGGCTGGTCTCGACCTCCTGACCTCGTGATCCACCCGCCT
    CGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGGGCCCGGCCTGTTTTTTCTTTATCTAAAAC
    TGGTCTGTAAAAATCTTTGTGTGTTAGAAACCATCTTCCAAATTCGTGTATTTATTTTAATCACTAAATA
    AATCTGATACTCATCGTGCAAAGCACTGTGTAAGGCACTGTTCAGGAAATAGATATATACAAAAAGATAT
    GTAAAGTTATTTACATTTAAATATGAAGTGTGTGTATCATAAGTATGTGTGTATTTACCATGTAGCTGGA
    GGAGTCAAGGAACAGAAAAACCCACCAACCAAACAAGTAAACACACACACCACATCATTCATATGCAAAA
    ATCCTTTATTGTTGAGGAATAGAGAGAGACATTATATTTTTAATGTAGGACACTACAGGAACTCTAGGGT
    ATTCTACAGTCAATGCCCATTGGGGTTGGGGATATTCACTATGCAGTTTCCACACCAGGGTCAGGTAGAA
    AAGATGGAGAAGGAGTCAAAGCACAAGAATGTTACAAGAACAGGGAAGAGAAAGGGTAACAGGAGCGTGC
    GCACCTGGGGATTGGGACAGGGAAAGAGGTCTTCCTAATGCAGTCCAGGAGTCCGGAAGTGGAGTACTGA
    GGACAAAAAACAAAACAAAACAAAAAAAAACACTGAAATCACATCTTATATCCTGTAAAAAAGTCCCACT
    GAAAACCTGCTGTGGCAGCAGGAGAGAAGGGGAGAGGCGTCTGTGAGAGAGGAAAGAACAAGTCTTGACC
    CTCAGCCTGCCCTCCAGGCTTTGGGCCTGAGGATGGAACTCTCCTAGCCGCAGGTACTTGCACCTTCCCA
    GCTGCCATATTTACATTACTCTACACACCTGTGTGCATGGCCAGTGGGGCCCATGTGGACCAAAGATGTA
    GGAACCAAGTTCATGCCATCCTAGCACCACCATGAGCCAGACACACACACAGCCCTGCCCAAGGTGCCCT
    ACTATGAATGCTCCCCCTGCCTTATCCAGTTCCTCCTCTTCCCAGAAACCTCCCAGAGGAAGCACAGGCC
    CAGGGATTACTTACAGTTTCACATGAATGAAATCAGATGACACAATGATCTGTGTTGATCCATCTCATCT
    AATCTTTAAATGCCAGCTCCCAGAAACCTTATGTCCTATTCTGCCTGACACCTAACAGGTTGGGAATGCT
    CCCTCAATGGTAAGATTATGAAAGCTGCAATTATGGGTCCCTCTGAAAGAGCCTGGATGCACAAACAAGC
    ATTAAAAGTAGATTTCCACAATGCACTTGTTTTTGCCATTTGTATAAAATACTATAGCAAAATTGCACTG
    TAATTATCTTTATGGCCAGTGCATAACGCTGGAGCAGAGTATGTCATCCAGATATAGCTGCTCGTATAAT
    TATCTGGCCGTCGGCAGGACTGAAATAACCATCTAATGAGAGCAAAATGATACAGTAACTACCATGTAAT
    CAGCAAGTTACAGTTATTATAGCTCTTTGTGCCCTCTCACCCAACACTAAATGGGGGGCCAGACTCAGTC
    TCTGGTCCCAGGAATTCATGCACAGGCAGCATCATCAAGCACCAGCTCCTTGGGTCTTGGGCGTGCCACC
    TTGAGACCAGTGAGTATGGGTAACAGGGACACAGAGAGAAAAGCAGGAGGCTCTCCATGATTACGAGCCT
    TCCCAATCTCTCTCCCTCTCCTTTTCACTCCCTCCACCCCAATTCTTTGGAAGCTAGTGAAAAGAAAGCC
    ATTGGTGGAGCTGCTCTTGCTGTGTGTGGGAAGACAGTGTCAAGGCGGAAGGTTCGTGTTTTGACTAGAC
    ACTTCCCTGGGGGACTCTTACACTCGCATACCAAGTGAGGAATTTGTCAGCCCACTCAGGCCTGGGGTCC
    AGACAGAGCCAGTCAGGGGAAACATTAGGACATCCCCCATACCTCCCAAACTGGGACAATTATCATAAAA
    TTGGCACACAGCACTGTGCACCCGGCACTGTTCTGAACACTTTACCTATACTCAATCCTCAAAACCACAA
    CCCTGTGAGATACTATTATCACTTTCATTTTTCAGATGAGGAAACTGAAGCACAGGAGGATATGTACCTT
    GGCCAAAAAGATACAGATAGAGTGGCAGATCCAGGATTCACATTCAGGCCATTGTTCTTATTCATTATGC
    TAAAGAGAGATTCAGATATGGTTTCCTGCTAATCCCCATTAGATTTCAGAGTTCCTTGCATTTCTTCCTG
    AATCATCCTTGTGTATGACTACCTTCTGGGCCCCGTAACCCAGGATTCTGAGTGATTTGAAAGGCTCAGC
    CTGCCTGGGAGGCAAATGCTTGCCTGTGAATTGGTTGTGGTATTTGTAATTATCAGATAATAATTTCTCC
    ATCTTTTTGCATAGATTACAAAAGAATGAAGTCCAAACAACAGAAATTGCCCATCGGGGAAGCCCCTCTC
    TCCTCATCTCCCAACACTCACTCCTGCTCAATAGTCAGCTGCCGTCCATGATCCAGAGGAAAGAGATGAA
    AGACGGGGGATGTGTACAAACACACGTGCCTCTAAGTGTCTTTGTGTTTGGCCGACAGTGTGCTGGGCAT
    TGGCAGTGTATTTCAGGGCAAATCTATGAGCTTTAATACCTGTAGCATGGGAGGAAATGCACAGCTGGCA
    ACCCCCACAGCCCCCTCCCCAGTCCCCTCTCCCCTCTCTAATGAACAATCCTTACCCTATTCTGCTCTCG
    GGGGATACTTGAGGATGCCAAAGCTGGGGACGTTCTCTTGATTCACATCCGTAGGAGAGTCTAGGGTAAA
    GCGAACAGTGTAAGTAAGAGGGATCTTTCTGAATGTGAACTACACCCTGATCCAACAGCAAAGGGCTGGC
    TATGACGGGCAGTGTTCAAAGACAGAGTTGGGAGACTGGGGTGGAAGCAGA
  • Protein Sequence : Show Sequence
    >gi|6005705|ref|NP_009195.1| G-protein coupled receptor 182 [Homo sapiens]
    MSVKPSWGPGPSEGVTAVPTSDLGEIHNWTELLDLFNHTLSECHVELSQSTKRVVLFALYLAMFVVGLVE
    NLLVICVNWRGSGRAGLMNLYILNMAIADLGIVLSLPVWMLEVTLDYTWLWGSFSCRFTHYFYFVNMYSS
    IFFLVCLSVDRYVTLTSASPSWQRYQHRVRRAMCAGIWVLSAIIPLPEVVHIQLVEGPEPMCLFMAPFET
    YSTWALAVALSTTILGFLLPFPLITVFNVLTACRLRQPGQPKSRRHCLLLCAYVAVFVMCWLPYHVTLLL
    LTLHGTHISLHCHLVHLLYFFYDVIDCFSMLHCVINPILYNFLSPHFRGRLLNAVVHYLPKDQTKAGTCA
    SSSSCSTQHSIIITKGDSQPAAAAPHPEPSLSFQAHHLLPNTSPISPTQPLTPS
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
13. GPX1
  • Gene Name : GPX1
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 2876
  • NCBI Protein GI : 41406084
  • Genbank Accession : AC121247
  • Protein Accession : NP_000572
  • Taxonomy ID : 9606
  • Chromosome No : 3
  • Gene Starting Position : 49394608
  • Gene Ending Position : 49395790
  • Gene Strand (Orientation) : -
  • Protein Name : transcript variant 1
  • Protein pI : 6.51
  • Protein Weight : 20647.83
  • Protein Length : 203
  • Protein Note : Also known as GPXD; GSHPX1
  • DNA Sequence : Show Sequence
    >gi|224589815:49394608-49395790 Homo sapiens chromosome 3, GRCh37.p5 Primary Assembly
    GTTCTGCTGACACCCGGCACTTTATTAGTGGGGAAACTCGCCTTGGTCTGGCAGAGACTGGGATCAACAG
    GACCAGCACCCATCTCGAGGTGGTATTTTCTGTAAGATCAGGTGTTCCTCCCTCGTAGGTTTAGAGGAAA
    CACCCTCATAGATGAAAACCCCCCCGAGACAGCAGCACTGCAACTGCCAAGCAGCCGGGGTAGGAGGGGC
    GCCCTAGGCACAGCTGGGCCCTTGAGACAGCAGGGCTTCGATGTCAGGCTCGATGTCAATGGTCTGGAAG
    CGGCGGCTGTACCTGCGTAGGGGCACACCGTCAGGGCCCACCAGGAACTTCTCAAAGTTCCAGGCAACAT
    CGTTGCGACACACCGGAGACCAGGTGATGAGCTTGGGGTCGGTCATAAGCGCGGTGGCGTCGTCGCTGGG
    AGCTGGCAGGGCCTCCCGCAGGAAGGCGAAGAGAGGGTGCGCCCCCGCACCGTTCACCTCGCACTTCTCG
    AAGAGCATGAAGTTGGGCTCGAACCCACCACCAGGCCGGACGTACTTGAGGGAATTCAGAATCTCTTCGT
    TCTTGGCGTTCTCCTACAGGAGAGAAGGGCAGCTAGAACCCGGGGTCAAGAGGAGGAGAGAAACGTTCTA
    ACCACAAACAAGGGAGATTTTCTATGAGTCACCGGGATTTTGCCCTCCATGCGCAATCCCAAGGGCGGAG
    AGGAATTTCAGCAGCTACGAGCAACAGAAAGGAAACGAGAGAGTAGCCAGACTCTCCGCGCATGGAGCCG
    ACGGCACCCACCAGCACACCGCCGGCGCCCCCAGCCACTACTGCACGTCCGCCCCCGCCCCGCCCCGCTC
    CGCCCGGCGCACCTGATGCCCAAACTGGTTGCACGGGAAGCCGAGCACCACCAGGCCCCGGGGTCCGAGG
    CGCCGCTGCAGCTCGTTCATCTGGGTGTAGTCCCGGACCGTGGTGCCTCAGAGGGACGCCACATTCTCGA
    TAAGTAGTACCTTGCCCCGCAGGGAGCCCAGGCTCACAGGCTCCCCGCCGGCCAGCGGGCGCGCCGAGAA
    GGCATACACCGACTGGGCCGCCGCCGCCGCCGCCGCTAGCCGAGCAGCACACATGGCGCAATTGTCCAAG
    AAGCCAGCGGAGCGCCCCGAACAAGCACTGTAAGGGGAGGCCAGCAGGCGCCTCCTTTTAACT
  • Protein Sequence : Show Sequence
    >gi|41406084|ref|NP_000572.2| glutathione peroxidase 1 isoform 1 [Homo sapiens]
    MCAARLAAAAAAAQSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLUGTTVRDYTQMNELQRRLGPRG
    LVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDA
    TALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
14. HIST1H2BE
  • Gene Name : HIST1H2BE
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 8344
  • NCBI Protein GI : 21396484
  • Genbank Accession : AF531288
  • Protein Accession : NP_003514
  • Taxonomy ID : 9606
  • Chromosome No : 6
  • Gene Starting Position : 26184023
  • Gene Ending Position : 26184457
  • Gene Strand (Orientation) : +
  • Protein Name : histone cluster 1, H2be
  • Protein pI : 11
  • Protein Weight : 13013.18
  • Protein Length : 126
  • Protein Note : Also known as H2B.h; H2B/a; H2B/g; H2B/h; H2B/k; H2B/l; H2BFH; H2BFN; dJ221C16.8
  • DNA Sequence : Show Sequence
    >gi|224589818:26184023-26184457 Homo sapiens chromosome 6, GRCh37.p5 Primary Assembly
    CATGCCTGAGCCAGCGAAATCCGCTCCCGCCCCGAAGAAGGGCTCCAAGAAGGCCGTGACCAAGGCGCAG
    AAGAAGGACGGCAAGAAGCGCAAGCGCAGCCGCAAGGAGAGCTACTCCGTATACGTGTACAAGGTGCTGA
    AACAGGTCCACCCCGACACCGGCATCTCCTCTAAAGCCATGGGGATCATGAATTCCTTTGTCAACGACAT
    CTTCGAGCGCATCGCCGGCGAGGCTTCCCGCCTGGCGCATTACAACAAGCGCTCGACCATCACCTCCAGG
    GAGATCCAGACGGCCGTGCGCCTGCTGCTTCCCGGGGAGCTGGCCAAGCACGCTGTGTCAGAGGGCACCA
    AGGCCGTTACCAAGTACACCAGCTCCAAGTAAACTTGTCCCTGCAACTGCCTTAGTAAACCCAAAGGCTC
    TTTTCAGAGCCACTC
  • Protein Sequence : Show Sequence
    >gi|21396484|ref|NP_003514.2| histone H2B type 1-C/E/F/G/I [Homo sapiens]
    MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDI
    FERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
15. HIST2H4A
  • Gene Name : HIST2H4A
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 8370
  • NCBI Protein GI : 4504323
  • Genbank Accession : AF525682
  • Protein Accession : NP_003539
  • Taxonomy ID : 9606
  • Chromosome No : 1
  • Gene Starting Position : 121215050
  • Gene Ending Position : 121215445
  • Gene Strand (Orientation) : -
  • Protein Name : histone cluster 2, H4a
  • Protein pI : 11.91
  • Protein Weight : 10474.58
  • Protein Length : 103
  • Protein Note : Also known as H4; H4/n; H4F2; H4FN; FO108; HIST2H4; HIST4H4; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4B
  • DNA Sequence : Show Sequence
    >gi|157704448:121215050-121215445 Homo sapiens chromosome 1, alternate assembly HuRef, whole genome shotgun sequence
    GTGGTCGGCCCTAAAAAGGGCCTTTGGGATCGAAACGTGCAAAGCTGGAGCGGCGGCCTAGCCTCCGAAG
    CCGTACAGGGTGCGCCCCTGGCGCTTGAGCGCGTACACCACATCCATGGCTGTGACGGTCTTGCGCTTGG
    CGTGCTCGGTGTAGGTGACTGCGTCCCGAATCACATTCTCCAGGAACACCTTCAGCACACCGCGGGTCTC
    CTCGTAAATGAGGCCAGAGATCCGCTTAACGCCGCCACGCCGAGCTAGACGCCGAATGGCAGGCTTGGTG
    ATGCCCTGAATGTTGTCTCTCAAGACCTTGCGGTGGCGCTTAGCGCCCCCTTTGCCTAAGCCTTTTCCGC
    CCTTTCCTCTGCCGGACATGACCGCTGGAGCCCGATAGACAGCTTC
  • Protein Sequence : Show Sequence
    >gi|4504323|ref|NP_003539.1| histone H4 [Homo sapiens]
    MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDA
    VTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
16. human IFNG
  • Gene Name : human IFNG
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 3458
  • NCBI Protein GI : 56786138
  • Genbank Accession : AC007458
  • Protein Accession : NP_000610
  • Taxonomy ID : 9606
  • Chromosome No : 12
  • Gene Starting Position : 68548549
  • Gene Ending Position : 68553520
  • Gene Strand (Orientation) : -
  • Protein Name : interferon, gamma
  • Protein pI : 10.01
  • Protein Weight : 18454.87
  • Protein Length : 166
  • Protein Note : Also known as IFG; IFI
  • DNA Sequence : Show Sequence
    >gi|224589803:68548549-68553520 Homo sapiens chromosome 12, GRCh37.p5 Primary Assembly
    AGTTGTGAACTTACACTTTATTCATATATATTAGATATTGATAATTTTAACAAATGAGTTACTTTCCATT
    TGGGTACAGTCACAGTTGTCAACAATATTTGGAAGCACCAGGCATGAAATCTCCTGAGATGCTATGTTTT
    CATCAGGGTCACCTGACACATTCAAGTTCTGTCTGACATGCCATTAAAGCACTGGCTCAGATTGCAGGCA
    TATTTTCAAACCGGCAGTAACTGGATAGTATCACTTCACTTATAAGTGTTCATTGTATCATCAAGTGAAA
    TAAACACACAACCCATGGGATCTTGCTTAGGTTGGCTGCCTAGTTGGCCCCTGAGATAAAGCCTTGTAAT
    CACATAGCCTTGCCTAATTAGTCAGAAAACAAAGGATTAAGTGAGACAGTCACAGGATATAGGAATTATA
    AATAATACATATATTAATAGATATTCATTTTCATTACACAAAAGTTGCTATTATAAATACTTATTTGATT
    GATGAGTCTAAAAATATATTCCCCATATAAATAATGTTAAATATTAATAAATAGATTTAGATTTAAAATT
    CAAATATTGCAGGCAGGACAACCATTACTGGGATGCTCTTCGACCTCGAAACAGCATCTGACTCCTTTTT
    CGCTTCCCTGTTTTAGCTGCTGGCGACAGTTCAGCCATCACTTGGATGAGTTCATGTATTGCTTTGCGTT
    GGACATTCAAGTCAGTTACCTATCGGGAAAGAAAAGAGCAAAATTAATTTCAGGCATATAAGCCATCAGG
    ATATTCTGCTAATGTCATGATGGTCAGTGAAAATAAAAGTATTCCTTTAAAAAATGGACCGTATTACCTT
    AATATACAATATTTACTTCTTCAGCAGTTGAAGCTAAAAATTACAATTACATTTCTAGTTGTGCTATAAT
    GTGGTGGAGTCCATCACATATCTTTAAATCTACTTCTATAGAATTCTTGAAATTATATAGTGAAAAGACT
    CCTGGTTGTGGAGTCAGCATAAAAAGGTGGGTTTGAATCTCATGCATGCAATAATTCTGACCTTGAGCCA
    GCTACTTAACCTCTCTGAACTTCTATTTTCTCATCTAAAGGAGGATGAGACTGTTTCTATGACCTCTCTA
    TCATCAATCTACCAATTATATCACTTTATACTTCAGTCTTTCCTGTATGCATCAAATGAGTAACTATAGG
    TCAATGATTCTCACTGTTGTGCATAGCAGATTAATCCGGAAACCTGTCAAAGACCTCAGAGATCCAGGTG
    CATGAAGTGGAAGGACTGTGCCCTGTGCATTCACTAAGCTCCCCAGGTGATTCTGACATCCACCTAAGTT
    TGAGAACCACTGATCTAGGTTAGGTACCCTAGAAAACACCAAATCCAAAACGAGTGAAAACTGTAATTTT
    TAATTTCTCCCAGATTAGGTCAGTCTCTCAATTTCCATAATAGATTTTAAAATAAACACCCCCAATCATA
    TTTATATATGGCTTACTGATATATAAAGTTTAAAATTAAGAATTGCAACAACTTTTCCAGTACCCTGCCT
    TCTAGGCAAGCAAATTGAACTACTTGCATCTCCTCACTCTAACCAATAGGGCCATTTAGATGATGCTTCA
    TAAAATGTGGACCATTTACCTTCCTCTTGGCTCTGCCATTACATTTTTTCTAATCTTGGGAACACTATGG
    CTACTCACCACATTTCTGAGGATGGCTGGGGGATTACAGCTAAAGACTAATTGTCAAAGGGCATAACATG
    CATAAAATAGCTTAGCAACTGAGCCAAAGAGAATAATAATTAATAGAGCTCACTCAAGATTGCCCATCAA
    GAAACAGGAGGTTTGTAGCTTCCAGAAGGAAGAAACCACAGATTTTTTTTCATTTTTTTAAGGCCCAGTT
    CCTGCAGAGTAGAAAAAATTTTCGACAAGCTGTGTGTTTGCAAGTGACAGATTTTACTTTCCAATGTTCA
    AATCATCACCAAGAAACTAAGAGACTTATCCAGGACTGAACAGTAAGCCAGGGGCACCACTGGATTAGAA
    ACAAGTGCTGAGTGTCTAGTTTATAACCAAAGGAGACCTGTTGCCATGTCTCTCCTTTCTGTTTCTGGGG
    GCTTACATGAGAGGAGTGAGAGGCAGGCCCAGCAATAGTGAAAGCTGAGCACATATCCTAAGAACACAAA
    TGCTAGCTTGAAGACCCTGGTCCCTCTCATTTGAGCCCATTTATATCAAAGTCAACCCCAAAGGAATCTA
    GAGGCCTTTGTCTTCTAGAAAACATTTTGAAAAACATGATTTGTCTTTATGCCTCAACGTTATCTTTCTG
    TTTATAGAAGCCATAACTTAAAGATACAAGTTCATGAGGTAAGACTTAGGAGTCTAAGGAGTTCTCCAGC
    TGAGATATTCTGAAAGTTGATAGAGAGTTGATAGAACAATCTTTTCATAAGGTATAAATTCTATCTATTT
    TTCCCTAAAAACAAACAAACAGCAACCATTCTTGCTTCTAATTGGGCAGTACAATCTGATAGGTTGGCTA
    GAGACTTGCAGTGGGGTGTCCCTGGTACCTATTCAAAGACTGTAGCTTTCTTCTATCTCATTCTCATTTT
    CTATTCTTTGCATTGTAGAGTTTTGGAGCAAAGAAGGTCATCAAACTTATACAGTGAGCCTAACAGTTTC
    CTTTTAAGATGAGGAAACTGAGCCCCAGCCAGCCATGTGATTCATCACAGTTCCTTGGTGGCTGAGTTGG
    GAGGAGAACACACATCTTCTCAGCTCCTCCCACTGCTCTTTCCATTAAGACAGACAGCCTCTCATTCAAA
    GTAAGAGAATTTCCATCATATGAGCAAGGGACAATGAGAGAACTGCTTCTCAGTACTCCCCGCTTCTTCC
    TCACCTACTTCCTCTTCACTGGATTTGTCAACTCACCTGTCTTTACGCAATAGTTACAATGCCAGCATTT
    CTCTACATTACATACTTCAGCGATTCTCTTACTGGCTTTGCAAAGTCACCCAAACACGAATGGAAATAGT
    AAGGTAGAGTTTCAGCCATGAGTTGAGTTCATAGCTTTAGCAACTGTTAAATAGCTAATGTCTACTTTCT
    GGAGAATAAATGCTTTGCAAGACCCTCGGCAATGAAACCAAAGAAAGAATTTAAATAGCCTCACCGAATA
    ATTAGTCAGCTTTTCGAAGTCATCTCGTTTCTTTTTGTTGCTATTGAAAAACTTGACATTCATGTCTTCC
    TTGATGGTCTCCACACTCTTTTGGATGCTCTGGTCATCTTTAAAGTTTTTAAAAAGTTTGAAGTAAAAGG
    AGACAATTTGGCTCTGCATTATTTTTCTGTCACTCTCCTTGGAAGGAAAGAGCACAAACAGAGGATGATG
    TGAATTTATCCATCAGAAAGCAAGCAACAGGAAAATTAGCCAAATGGGAATATTCAGCTTACCTCTTTCC
    AATTCTTCAAAATGCCTAAGAAAAGAGTTCCATTATCCGCTACATCTGAATGACCTGCATTCTAAAAAAA
    AAAAAAGAAAAAATTGGTTTACAATTAGCCCATAAATTGCCTTAAAAATATATTTCAAGTTTCATTGAAC
    TCAGATGTGACAATATTCACTGATTTCCTTTTCAACTCTTCTGCTTAGTTCTAACAATAAGTATTCCCAA
    AAGGCTTATGTGAGATATAGACAAAGACTATTATGTTCTTTTAGCTTTTTTATTTCCCAATATAACCATT
    AAATTGCAATGTCACAAATGAGCTCAACAAAGCTGATACTCCAAAGGTCCCAAAAACTATAGTAAGCTAA
    AGAAAGTATTTTCAAGCTAGGCTAAAAAATACAAAAACAAAAAACAGCAAAGCCACCCCACTATAAAATA
    CTGCCCCCCAATGGTACAGGTTTCTATTACATCTACTGTGCCTTCCTGTAGGGTATTATTATACGAGCTT
    TAAAAGATAGTTCCAAACATGTGCGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATTTGATTTTGTGTT
    GTAAGAATAAAATCAATTGTGAATGTCTGAATATTATTATGACAGCTATAATTATAACAGCTAAGTTTTA
    AACAAATAATGAAATATTTAAAATGTAAAAACTTTTCATTAACCATCAATAAAATTTTAAAAGAAACTCA
    AAAAATTCACTTAAAATTTTTAAACATTCCTTAGCCAGAATCATAATTCTTCCAAAATCCCAGTGAGCAA
    CGAAGTTCCGGAATACTTCACAAGTAATATAGATAGAGAAGCAGAATGTTGAAATCACTTTTTGGAGAAA
    ATCACCTATCAGTTATTTTTTTAAGCCAAAGCTTTAAGGACCTTTTTGACTTTAATTTGTAAACATTTCC
    TATTACCTTTTTAATGCTAATCTTGACCTTAGAGTCTGAGAAGTCAAAAACTAATAACCAATCATTTTTT
    TCAATCTGCATACTCCTTGACTATCACAACTGAATGAGTTCCCACCACAAAATTATTAACATCATTATGC
    TTCATACCCATATTATGCCCATCTTTTGGAAAATTCATAAATCCCTTTTAAGTCTCTATACAAACTGAGC
    AGAAGGTTAAAACAATATCTAGATTATTTAGTCAAGGAACTCATCTAGTCAATAATTAACAAAATAACAC
    CAAATCTCAAAATGACTGCCTACAAGAGATGACAGCCTATCAGAGATGCTACAGCAAGTCGATATTCAGT
    CATTTTCAACCACAAACAAGTACTATTAAAAAGTCATACTTACAAAATATTTCTTAAGGTTTTCTGCTTC
    TTTTACATATGGGTCCTGGCAGTAACAGCCAAGAGAACCCAAAACGATGCAGAGCTGAAAAGCCAAGATA
    TAACTTGTATATTTCATCGTTTCCGAGAGAATTAAGCCAAAGAAGTTGAAATCAGTAGTTCTTGTATCAA
    GCTGATCAGGTCCAAAGGACTTAACTGATCTTTCTCTTCTAATAGCTGATCTTCAGATGATCAGAACAAT
    GT
  • Protein Sequence : Show Sequence
    >gi|56786138|ref|NP_000610.2| interferon gamma precursor [Homo sapiens]
    MKYTSYILAFQLCIVLGSLGCYCQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQS
    QIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVM
    AELSPAAKTGKRKRSQMLFRGRRASQ
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
17. IFNG
  • Gene Name : IFNG
  • Sequence Strain (Species/Organism) : Bos taurus
  • NCBI Gene ID : 281237
  • NCBI Protein GI : 31982949
  • Locus Tag : BOS_5228
  • Genbank Accession : AF140653
  • Protein Accession : NP_776511
  • Taxonomy ID : 9913
  • Chromosome No : 5
  • Gene Starting Position : 45830157
  • Gene Ending Position : 45834980
  • Gene Strand (Orientation) : +
  • Protein Name : interferon, gamma
  • Protein pI : 10.24
  • Protein Weight : 18866.5
  • Protein Length : 166
  • DNA Sequence : Show Sequence
    >gi|258513362:45830157-45834980 Bos taurus breed Hereford chromosome 5, Bos_taurus_UMD_3.1, whole genome shotgun sequence
    AATTAGAAAAGAAAGATCAGCTACCTCCTTGGGACCTGATCATAACACAGGAGCTACCGATTTCAACTAC
    TCCGGCCTAACTCTCTCCTAAACAATGAAATATACAAGCTATTTCTTAGCTTTACTGCTCTGTGGGCTTT
    TGGGTTTTTCTGGTTCTTATGGCCAGGGCCAATTTTTTAGAGAAATAGAAAACTTAAAGGAGTATTTTGT
    AAGTATGATCTTTTAATGAGACTTTCTTGTGATTGAAATGACTTGATGTTTGATGTTGACTTGGAATTCT
    ATCTATGATGGGCTCTCATCTCTAGTCTTCAGTCATTTTGAGAAGACTTGCTGTTATTGTGACTGTTAGC
    TAGATGTGTTTGTTTGTTTGGTTTTTTTTTTTTTTCTGACTAAACAATCTCTGCTCAGTTTGCTATAGAG
    ATTTAGAAGGGATTCATGAATCTTTCAAAAGATGGGCATAATATGTGTATACATCATAGTGTTGATATTT
    TGTGATAAGAATCCATTCAACTATGATGTTAAGTAGCAAGCATACTGAAAAGACAGCTGAGTCTTAATTT
    TTGATTTCTCATGTTACTATAATGATGACAGTCAGAAGTCAAGATCAGCATTAAAAATGGTCATTTGACA
    TAATTGATCAATTAAAGAAGATGAGGTCCTTAAATATTTGGCTTAAAAACAAAAACACTTATAGGTGTTT
    TTCTCTAAAAAGTCATTTTAAAATTTTACTATCCCATCTAGATTGTCTGTGAATTACTCTTGGACTCAGT
    CACTTGCTGAGATTTTTTTTGTAGGAGTTATAATCTAGTTTATATTTTAAAAAATTTTTTGTCTTGTTTT
    AGGAATTTCTTAAAAATTTACTTATGGTTAATTAAAATAGCTTGGGCATTTTAAATATTTTATCATATAT
    CCAAATTTAGCTGTTATAATTATAGCTGGAGCTATCTTTTAAAGGCGATATAATACCTTATAGGAGGACA
    CAGGTAGAGGTTATGAACCGTATACCATAGGGGGGCAGTATTTTATAGTGAGATGATGTTGTTGATTTTT
    TTTTTTTTTTTAACTATACCTTTTTCTTACCATAGTAATTTGGGACAGTTGAAGTATCATCAGTCTTGAA
    TGAGTTCATTGTGATGTTATATTAGTAAATAAGAAAGCAAAAATCACATGATAGTCTTTGGCTGTTATCC
    AACCAGGTAGCTTTTGTGAATATTTTTTGTTAAAACTAAACAGTGTTGAAAAGAAAATCAGTAAATAGTG
    CCAGCATCCAAGTTCAATAAAACTTGAGGTACATTTTTACAGCAATTTATGGACTAATTTTAAGCCAATT
    TTTTATTGTGTTGTTTTATTTTCCAGAATGCAAGTAGCCCAGATGTAGCTAAGGGTGGGCCTCTCTTCTC
    AGAAATTTTGAAGAATTGGAAAGATGTAAGCTGAACATTTCCATTTGGCTGATTTTCCTGTTGCTTTTTT
    TCTGATGGATAAATTCACATCAACCTCTCTTTGTGCTCTTTTCTCCCAAGGAAAGTGACAAAAAAATTAT
    TCAGAGCCAAATTGTCTCCTTCTACTTCAAACTCTTTGAAAACCTCAAAGATAACCAGGTCATTCAAAGG
    AGCATGGATATCATCAAGCAAGACATGTTTCAGAAGTTCTTGAATGGCAGCTCTGAGAAACTGGAGGACT
    TCAAAAAGCTGATTCAAATTCCGGTGAGATGATCTTAATGCTTTCTTTGGTATCATTGCAGAGGCTCTTA
    CAAAGCACTTCATTCCCAGAAAATAGAAATTAGATACCAGGTGTAAAGCTCTGAAGTGAACTTGTGGTCA
    AAATGCCTCTTTGCTAGTTCTCTTTTGACTTGGGTGACTTTGCCGAGTCAGTTATTGGAGGCACTTAAGT
    CTGTGATTTTGGGAAAATACTGGCATTATGCCTATCGCACAAAGATGGGTGAATGGACAAATCAGTGAGG
    AGGAAGCAGAGAAGGAGTTGGGGTGATGGGGACAGAGTACTGAAAACCAGTTCTCCCATTGCCCCTTGTC
    TGTGTGTTGGAAATTCTCTTGCTTTGAATAGGTTGCATGTCTTAATGGAAAGAGCAGTGGGAGGGAAAGG
    GAAGAAGATGTGTGCTCCGCCCAGCTTAGCCACCAAAGAAATGTGTGACTTCAGATGAATCACAGGCCTG
    GCTGGGGCTGTTTCCTCATCTTAAAAGGAGCCTGTTGAGTTCACTGTAATTTCTACGGTCTCCTTTGCTC
    TAAAATTCTACAATGCCATGGAAAGAAAATGAGAATGAGATAGGAGAAAGGAATAGCCTTTGAGGAGACA
    AAGGCCCTCCCACTGTCGAACTCTGGTCAATCACCCAGATTGTATAGCCCAAGGAGAGGTGACTGCGTTT
    GTTTTTAGAAAGAAAATAGGTAGAATTGTCCTATCAATGAATTTTTAAAGTTTTTAGAAGCTGAAAGATC
    ACTTTGAAGCATAAGGACAAGTCACATTTTCCAAAATTTCACCTAAGGGACAACGGTCTCCAGATTTTTT
    TGCGGGGGAGAGTTGGCTTTTGTATAAATAGGCTTGGGTGAGAAGAGGAGGGGTCCCATTATGACTTGTG
    CTTTCAGGATTTGTACCAGCTCTTGCTCTAACCTGGTCTGCCTCTCAACAATCTCATATAAGCTCCCAGG
    AACTGAAATAAGTGGTTGCTCTTCAAGATTTCTCTAGTCTCCCATATATTAGATGGTATGAGAGTGGCTT
    GGAAAAATTATATGTAATGTTTTCCATGTTCAGCAGATCTCCATTGGTTGCAGGCTAGAGAATGAAAAGG
    CACTGTTCCTGACCCAGTGCTGCCTCTGGTTGCTGTTCAGTCCTAGAGAAGTCCCTTAGTTTCTTGGTGA
    TGCTTAGAAAACTGGAAAGTTATCACTTGTAAACACACAGCTGGTAGAAATTTTTCTACTCTGTTGTAAC
    TGGGCCTTGAAAACAATAGGTTGGTCCCATCCTTCTGGAAGCTCCAAGCCTCCTTCTTCACAATGTGCAA
    TCTTGAGTGAGTGCTCAACCATTGTCGTCTTTTGCTCAAATGTGAACATATTTAAGGCACGTTTTGCCTT
    TTTGTCTTGTAACTTAGAATCTCCTAGTCATTCTCAGAAATGTGGCAAGGTGGCTGCAGTGTTTCAGCTT
    TAGAAAGAAATGTAGTGGCAGAGCAAAGATGAAAGCAAATGGTCCTCATTTTATGAAGCATGAATTCACC
    TATATTGTCCTACTGGGAAGAGTGAGAAAATAGTTCAATTTGCTGGAGTGGAAGGCAGGGTACCGGGAGA
    TTGCTTTCATTTCTAACTTTATTATATAATACCAGTAATTGATTACTACCTTTAAATAAGATCCGTGTTT
    ATTTTTAATCCCATCATGGAAACAATGAGACTGACCTAATTTGGAGGAAATTAAAAATAATGGTTTTCCT
    CCTTCTAGAACTTGGTGTTTATAAAGTACTGAACCTATACCAATGGTTTTCAAACTTGGGTGGTGCCTAA
    ATCACCTGGTGATGTTGGTAACCACACAATGGCCCAGGCCTAGTCCGATTTCACCCATCTGGGTGTCAGA
    GTTCTTCATTAGGTCCTCAGGTTACTCTTTTACGCACCAAACATGAGAGCCATTGGCTATAGTAACTCAT
    TTGATGCTTACAGCGAAGACTAAGTACTAAGTGATAGCAGTGACGGAGACCCTAGGGATCATCTAAACAC
    TCTCCTCATACTTAGTGGAGAAAACAGAGATCTGGAGAGCCTCAGTGACTGGTTTAAAGTCAGATGTATT
    GCACACACAGAATTCACACCCACATTTTTCTGTCACTCTACTTCTGGGGTTTTTTCACTATATAATTTTC
    AGATCTCTGAAGTGGTAAATTTATGAATATGTGATGTGCTCCATCCTGTTACAGTACAACTATCAATTGA
    ATTGTTATAATTTTAGTTTTTATCACTGAAGAAACCAACATTACATATTAAGGTTCATTACTGCTAGTGA
    AAATAATTTGTTTTAAAGAATACTTTGATTTTCTTGGGCCTAGACAGCAGAATATCCTAATGACTCATAG
    GCCTGAAATTAATTTTGCTGTTTTCTTTCCCAATAGGTGGATGATCTGCAGATCCAGCGCAAAGCCATAA
    ATGAACTCATCAAAGTGATGAATGACCTGTCACCAAAATCTAACCTCAGAAAGCGGAAGAGAAGTCAGAA
    TCTCTTTCGAGGCCGGAGAGCATCAATGTAATGGTCCTCCTGCCTGCAATATTTGAATTTTTAAATCTAA
    ATCTATTTATTAATATTTAATATTTTACATTATTTATATGGGGGATATATATTTAGACATCAAAGTATTT
    ATAATAGTAACTTTTATGTCATGAAAATGAGTATCTATTAATATATGTGTTATTTATAATTCCTGTATCC
    TGTGACTATTTCACTTGACCCTTTTTTTTCTGAGTAACTAGGCAAGTCTATGGGATTTCAAGGTTTTATC
    TCAGGGGCCAACTAGGCAGCTAACCTAAGCAAGAATCTGTGGGTTGTGCACTTATTTCACTTGATGATGT
    AATGAATGCTGATAAATGAAATGATGCCATCTAGTCACTACTTATCTGAGACTAGATCTGGATTCTGAGC
    CACTACTTTGATGGCATGTCAGACAGCACTTGAATGTGTCAGGCTATATGACTTGTGCCCTGACAAAAAC
    ATAGCATCTCATCTCACCTTATACCTGGCGCTTCAGGATATCACTGACAATTGTGATTACACCCAAATGG
    AAAGTAATATGTTTGTTTAGTTTATCAATATTTAATATATATGAATAAAGTATAATTTCATAAC
  • Protein Sequence : Show Sequence
    >gi|31982949|ref|NP_776511.1| interferon gamma precursor [Bos taurus]
    MKYTSYFLALLLCGLLGFSGSYGQGQFFREIENLKEYFNASSPDVAKGGPLFSEILKNWKDESDKKIIQS
    QIVSFYFKLFENLKDNQVIQRSMDIIKQDMFQKFLNGSSEKLEDFKKLIQIPVDDLQIQRKAINELIKVM
    NDLSPKSNLRKRKRSQNLFRGRRAST
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
18. Ifng (Interferon gamma)
  • Gene Name : Ifng (Interferon gamma)
  • Sequence Strain (Species/Organism) : Mouse
  • NCBI Gene ID : 15978
  • NCBI Protein GI : 33468859
  • Genbank Accession : NM_008337
  • Protein Accession : NP_032363.1
  • Other Database IDs : MGI:107656; UniProt: P01580
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • DNA Sequence : Show Sequence
    >gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
    ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
    GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
    GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
    AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
    AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
    GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
    AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
    GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
    AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
    AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
    AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
    TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
    TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
    ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
    GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
    AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
    TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
    AGTATACCTTTGCAACC
  • Protein Sequence : Show Sequence
    >gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
    MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
    ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
    LLPESSLRKRKRSRC
  • Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
  • Additional Molecule Role : Vaximmutor
  • Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG) , M. tuberculosis DNA vaccine pAK4-sod , M. tuberculosis HBHA Protein Vaccine , M. tuberculosis phoP mutant SO2 , MVA/IL-15/5Mtb vaccine , Mycobacterium tuberculosis drrC mutant vaccine , Mycobacterium tuberculosis fadD26 mutant vaccine , Mycobacterium tuberculosis mce-2 mutant vaccine , Mycobacterium tuberculosis mce-3 mutant vaccine , Mycobacterium tuberculosis RD1/panCD mutant vaccine , Mycobacterium tuberculosis sigE mutant vaccine
19. IL-1b
  • Gene Name : IL-1b
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16176
  • NCBI Protein GI : 6680415
  • Locus Tag : RP23-384K11.2
  • Genbank Accession : AL808143
  • Protein Accession : NP_032387
  • Taxonomy ID : 10090
  • Chromosome No : 2
  • Gene Starting Position : 129364578
  • Gene Ending Position : 129371119
  • Gene Strand (Orientation) : -
  • Protein Name : interleukin 1 beta
  • Protein pI : 4.31
  • Protein Weight : 29045.55
  • Protein Length : 269
  • Protein Note : Also known as Il-1b; IL-1beta
  • DNA Sequence : Show Sequence
    >gi|372099108:129364578-129371119 Mus musculus strain C57BL/6J chromosome 2, GRCm38 C57BL/6J
    GTTTGTTTGTTTTAATGAAATTTATTTCATACTCATCAAAGCAATGTGCTGGTGCTTCATTCATAAAAAG
    TCTCATCCATATTATAGGAACCTATTTATTCTCAGCTTCAATGAAAGACCTCAGTGCGGGCTATGACCAA
    TTCATCCCCCACACGTTGACAGCTAGGTTCTGTTCTAGAGAGTGCTGCCTAATGTCCCCTTGAATCAACT
    TAAATAGATCAACCAATCAATAAATACATAAATAAATAGGTAAGTGGTTGCCCATCAGAGGCAAGGAGGA
    AAACACAGGCTCTCTTTGAACAGAATGTGCCATGGTTTCTTGTGACCCTGAGCGACCTGTCTTGGCCGAG
    GACTAAGGAGTCCCCTGGAGATTGAGCTGTCTGCTCATTCACGAAAAGGGAGCTCCTTAACATGCCCTGG
    GGAAGGCATTAGAAACAGTCCAGCCCATACTTTAGGAAGACACGGATTCCATGGTGAAGTCAATTATGTC
    CTGACCACTGTTGTTTCCCAGGAAGACAGGCTTGTGCTCTGCTTGTGAGGTGCTGATGTACCAGTTGGGG
    AACTCTGCAGACTCAAACTCCACTTTGCTCTTGACTTCTATCTTGTTGAAGACAAACCGTTTTTCCATCT
    TCTTCTTTGGGTATTGCTTGGGATCCACACTCTGCAGAAAAGTAGAAAAAAGAATTGTGTTAACGTCTGT
    GTCCGTGTGCATGTTGCATGTATGACAGAAAGGATAAAGCTACCACGGTAGACATATTCTCAGGGCTGCT
    GGAGAAGAGAATGAAAAAGAGGTTGTGGAACTTGAGGCCACATTCTTTACTGCTGAACCCCATCTTTCTA
    TAATCACTGAAGGAACCTTTGTTATATATATATTTTTTCTGAATCCTACCATATGGGTGAGAATTATATT
    AAAGCTCCCAAGGGTACCCAGTTTCCAGGCATTTTTGTCACAGTTCCCCCTAAATCCCTGGATAGAGTGG
    CATCAGCTTGGAAATGAAGCTTTTGTTAGGAATGTCACAAGTGCCAGTACAGGTCTGAGGATCGTTGGTT
    GAACCCTTTCACTGCATTCTCCAAACTGTTCTCTGTCAAAGGAGGAAAAACTGTCACCATATACAACTCT
    TTCATCCTTGATGCTACGACTTCTGTTGGGGGTGATAACGGTGGCCTGACATGACAACAGCATCAAAAAG
    GATTGATTTCCCATGGTGGCAGTGAGGTTTATGTGCACTTAAAAGTTATGCTAATTTTATTCTATATTTA
    TGTAAATCGTCAAGAAGACCTCTAATGTTTTCTGGCGGGTCAGGCAGGTGGATGTCTGGGAAGTAAGTAA
    AGGCAGAGTCTTCGGTGAGCAGTGGGGGAGGCTTCTCTACTGATGGACTCACCTCCAGCTGCAGGGTGGG
    TGTGCCGTCTTTCATTACACAGGACAGGTATAGATTCTTTCCTTTGAGGCCCAAGGCCACAGGTATTTTG
    TCGTTGCTTGGTTCTCCTTGTACAAAGCTCATGGAGAATATCACTGGAGAAAGGAGAGGAGTCTGGGTCA
    AGGGAACAGCAATGCAGGGTGGGCTCAAGCATTAGACGTGACAGGCAAAGCCTATGCAGTCCCCCGTGTG
    AACCCAGAATGAGCCTCCTCAGACACAAGATATGGACTAGCAGGGCTATACAGTCATAGTCATAGACAGT
    AGCAAGGTCAGTAGGCCGATCCAGGATTCCCTCAGGACCTCTTAGGAGCAGCAATGAAGAACGAAAGGTG
    TCTAACTGTTAAGCTCTAAGGGCTAAAGCTGTTATGTGGCTGGCAGGCTGTGTGTCCGTCTACGTGAGAA
    GTACAGTTTGGTCGCTATGAAAACAAAGCTGTGCTCCTAAAAGGACCTAGGGGCCAGCACGTTTTGAGGA
    AAATAGAGCAGGAGGCAGGTAGAACTCTATTAGCTGGGGAGAGTGGCTCCAAAAAAGAGACCCCCCCCAC
    TTTGATCTTAGCCACAGTACCTCAATTGGAGGCCCCTGGCTGCTTTTATGACTTTATCAAGAAATAAGAA
    TTATTCTTTTTAAAAGGTATAAAGCAATAAGATTCTTTTCACCTGGACCAGAGTAGACAGCTCCCTCCAA
    AGCTTCTAAGATCAGAAGCTAAAAGGAGACAGCACACACTGCTGCTGTTAGCTCTTGTCCCCTCAGCTGT
    GGTGGTGTGGTGCCAGGTCCACACCATCATCTAAAGAGGCCCTGACCGCGCTTGGAATGCTTGTTATTAT
    GGAAGAGAGATGTATGCTCCTGATAGTCAGCAAACCTCCCTTCAAGGAACTGTGTGTGTGGTATTGGTGG
    GAAGGATATCAGCACGAGGGATAGGACTGGGAACAAAAATGGACATATCAATTCTCTACCATCTACATTT
    CAGCCTCAAATGAGGGAAAAAGAACCCACAGGCAGTTATTGCATGTCCATCAAATGACTTCACACTAATG
    TGTTATCCCTATATGACTGGGCTGGAAAAATGGTCCTTTTTCGTTCTGTCTCTGTCTCTGTCTCTGTCTC
    TCTCTCCTTCCTTCCTTCTTTCCTTTCCCTTTCTTTTTCCTTTTCTTTTTTTGTATAATGACCATAGTGT
    TCCATTTTAAAACACAGGGACCCAGTAGGCTGCCAGGAGGCCAGAAAGGGAAACCAAGATGCTCCTACTT
    ACCTTGTTGGTTGATATTCTGTCCATTGAGGTGGAGAGCTTTCAGCTCATATGGGTCCGACAGCACGAGG
    CTTTTTTGTTGTTCATCTCGGAGCCTGTAGTGCAGTTGTCTAATGGGAACGTCACACACCAGCAGGTTAT
    CATCATCATCCCATGAGTCACAGAGGATGGGCTCTGAAAGTTCAAGAGCAGATGTTGTTTAGGCAGATAA
    TTAGAAATGCAAGCACTGTGAAGTGAAGCAGCAGAATTTGGCTTATTGAGCAACACCTAAGCATGTGCAG
    TCAAGATTCTATTCTAATTTTAGTGGGGGTATAAAAACACTGGGAGTGGGCTGGGCAAGAACATTGGATT
    TGGCATCGTGAGATAAGCTGCAGTGTTATAACCATTCACTAGGGACTAGCAGTAGTGGTCCTTGCATGAA
    ACGATGCCAGTAATAAGACCAGCTTAACTGGGAAATTGTGAGATTCACAACCTCTAGTATAAGCCAAAGT
    ACTTGGCAAGTCAATGGTTGAGTTGCAAGTGTAAGCATCATTAAAGCCTTTTTATAAGTTAATCAGCTCA
    AGATAGAACTGATTTGTTTGGAGGTTTCCTCATTTTCTTCTAATGGTTGGAATGTCTAGCACTAAGGGTC
    TTGCACTTGAGGGGCGTTGAGTTCTAAGAGGTGGGCTGAGAAGGACCAGAGCGAAGGTCTGCTGTCTGCT
    CTCAGTCCTCATTAAGTACCTTCTTCAAAGATGAAGGAAAAGAAGGTGCTCATGTCCTCATCCTGGAAGG
    TCCACGGGAAAGACACAGGTAGCTGCCACAGCTTCTCCACAGCCACAATGAGTGATACTGCCTGCCTGAA
    GCTCTTGTTGATGTGCTGCTGCGAGATTTGAAGCTGGATGCTCTCATCAGGACAGCCCAGGTCAAAGGTT
    TGGAAGCAGCCCTGTGGAGAAACCAGGCAGTGAGCACATCAAGTCATCAGACCAGAGGACTCCTACATCA
    ACCCCAGAGAACACCAAGGGCCCGGGAGATAAGTTCTTAACCACAATCCCTGTCTATGTGGAGCCCAGAG
    AATTTAATTGGCCTACCCAGGGGTACATGGCAACCAGACACAGGCTTCCTGGCATTCTCTATAGTAAACC
    TCATTTCTACTTTATCTTTCTTGTACAGACCTTGCCTCACTGTATAATTAGACCTACTTAAACGTTTCAT
    CTTTTGGTATCCTTGCTGCTCAAACATCATGTTATTTAATTTCTCATGACAGTATGTTCATGTTGTCTTC
    TATTCAACGGAGATCACCCAACAGGCTCTCAATGGCTTCCCATACATTCTTTTTCTCATAGGAATATTTA
    GCAGACACTATAAGCATAGCTTTTGTTTGTAAAATATGTCACCACTTTTCTGTTCTTTGGAGAAACACCA
    CATTAAGGCCTAAACACAGTTGCCTTGAGGCATGATAACAGTTTCAAAGCTTGAAGGACAGCGATAGATC
    ATTTCAATAGTGTAAAGGAAAGGTTGATTGGGAGAGAAGTTTCTGTCCAGAGTAGAGGCTCCCAGGGAAA
    GCCATGTTCTGGAGCAGGCAGTGGGGGACAGAGCCTGGCTGGGAAAGTGAGAGGCGTCACCCAAGGCTGA
    ACTAACTAGTTAGAGTGGTCTTAAAATACATTCATGTAAGCTAAGGACTAGATTCTGTAGCTGGGGGAGA
    CTGTTGAGGGATTGGGAAGAGGTCTGAGAGAAACAGCTGCCTCCTGAGTGGGGCAGTGACGTGGCTGGAC
    TGACGCCATGTGGCATTAGAGCGTTCTCAGTCATCATAGGAAGGGACAGCTGGGATGCAACATGGCTCTT
    CATGAGAGCAAGTCTGGAAAGTGCCCTTGTGCTAGGATGGACTTGGCCAGCACTTAAAACACTTCAGAAT
    GGAAGGTTGTGACAGATCGCCAACATTTCAGAGATCTTCTGACCTGTGTTTCCCAAAGGACACAGTGGGG
    ACTGTCTATGATCACACAGCAATGAAAGGTCTTGTCTAAGGCCACAGGTTATTTTTAAAAATCCTAACTA
    ACTTTTTGTTTATATCACTTATTTCTTATTATCTTAATCTCCCCAGGAACATGCGAAACTGTTCTTTGGC
    AGAGAAGGAAGAGATGCTAATTCTGAAGGTCATGCTCTCTAGATCCAGAGTTCGATTTAAGTGGTCAGAT
    TAAGCTCCATCTCTCTCAAGGGGGGACTAAATGTATGTTGAAGACCATACTGTCTTCTTAGCATAAAACA
    CCATGATTGCCATCAGCAAGCCTTGGTGTGTGGCTGTGGTAAATGAAACCTGTGTGGGAGCCAGGTCAGT
    GTGTGGGTTGCCTTATCTATGTCACTGAAAGAGGCAAAATAATCCCATCACAAAATAGCCTGGGAGCAAG
    GCAGTCCCATATCCCTTCCCGTTTGGGTTTTTTTTTCCCATGCCACAGTCCCTCCACAGGTTAGGAGCCA
    AGCTCAGAGTCTCACCTTCATCTTTTGGGGTCCGTCAACTTCAAAGAACAGGTCATTCTCATCACTGTAA
    TGAAAACAGATGTGGTCAAGTGATAGCTCCTTATCAATACTGAGCAGACAGACTTCCATTGTTCATAAAA
    CCACACCTACCATGACTCTTAAAGCTTCCCAAAGACTTCTGGGAAGTTCTACAGTGCTACGTAAGTAACA
    GTCTAGAAGCAGATTCTGGGCCAGGTAAGTGGGTGCTCTGGTTGCTCTCTGTTGACTTCTGACACCTAAA
    TTCTAGACTAGCAGAGTTAGCTAAAGTCTGAGCTCTCTGCCTTTGCTTCCAAGCCAGACTTTGCAAAAGA
    GTCTCTTATCCTGTCCATGTGGAAGTTTTTCTTACCATTGCATCTGTAGGAGTCCCTGTAGTGACAGCAC
    CTAAGTCCCTAGGTTGGGCTTGGGAGTGAAGAGGTCAGTCTTTAAGCTAGGTTGCTTGAACTCTGATAGC
    CACTGAAGGAGATGAGTTGGGGACTCTCTGGCCTCTGAAAGTAGATAGCCCTCCATTCCTGAAAGCAGAG
    TGCTGGATATGTGACCACTCTCCAGTACCCACTGACCTGTCAAAAGGTGGCATTTCACAGTTGAGTTCAG
    GAACAGTTGCCATAGCTGCTTCAGACACCTGTGTGAAGAAAGAAAGTGGGTGGGCATCCAGCGTTAGCTC
    CCCGAGGGGCGTCCTTCATATGTGTTTCTCTTAGCTCTAGTTCAGGGCAGGCATGCTAAACTGGTCTCTG
    AGGAATGAAGGAACGGAGGAGCCGTTGTATAGGTCTAGAACAGTGGCCCTTTTGGAGTCAAGTGGCCCTT
    TCACAGGGGTCACCTAAGACCATTGGATAACACAGATATTTACACTTCATAACAGTAGCAAAATTATAGT
    TATGAAGTAGCAATGAAAATAATTTTAGGGTGTGTGGGGGGTCACTACAACATGGGGAACAGCATTAGGA
    AGATGCTCTGGAAGGAAGCAACAGCAGAGCCAAACCCTAATAAAGAAGCTTTCCTACAAGACAGGAGCTG
    CCTTTTAGATGTCAGCGGCTATACAGACAGTACTCATTTCCAGAGTACTTCACAAACCATGGAGGTACAG
    CTACTTTTTATAAATAAAAAGCCCAAAGTCCATCAGTGGGGGGGGGGGAAAGAGAGAGAGAGAGAGAGAC
    AGAGAGAGACAGAGAGAGAGACAGAGAGACAGAGAGAGACAGACAGAGAGAGACAGAGACAGACAGAGAG
    AGAGAGAGATAGACAGACAGAGACAGAGAGAGAGACAGACAGAGACAGAGAGAGAGAGACAGAGAGAGAG
    AGATAGAGAGAGACAGAGAGAGAGAGAGACAGAGAGAGAGAGAGACTTACTTGCACAAGGAAGCTTGGCT
    GGAGAGGATCCCAGATGAGCCTATTAGGCCTC
  • Protein Sequence : Show Sequence
    >gi|6680415|ref|NP_032387.1| interleukin-1 beta precursor [Mus musculus]
    MATVPELNCEMPPFDSDENDLFFEVDGPQKMKGCFQTFDLGCPDESIQLQISQQHINKSFRQAVSLIVAV
    EKLWQLPVSFPWTFQDEDMSTFFSFIFEEEPILCDSWDDDDNLLVCDVPIRQLHYRLRDEQQKSLVLSDP
    YELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKK
    KMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
20. IL-6
  • Gene Name : IL-6
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16193
  • NCBI Protein GI : 13624311
  • Genbank Accession : AC112933
  • Protein Accession : NP_112445
  • Taxonomy ID : 10090
  • Chromosome No : 5
  • Gene Starting Position : 30013160
  • Gene Ending Position : 30019967
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 6
  • Protein pI : 7.5
  • Protein Weight : 23291.86
  • Protein Length : 211
  • Protein Note : Also known as Il-6
  • DNA Sequence : Show Sequence
    >gi|372099105:30013160-30019967 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
    ACCAAGAACGATAGTCAATTCCAGAAACCGCTATGAAGTTCCTCTCTGCAAGTAAGTGAAGGCAGTTCCT
    TGCCCTCTGGCGGAGCTATTGAGACTGTGAGAGAGGAGTGTGAGGCAGAGAGCCAGCATTGTGGGTTGGC
    CAGCAGCCATCAGCTAGCAGCAGGCGCCCAACTGTGCTATCTGCTCACTTGCCGGTTTTCCCTTTTCTCC
    ACGCAGGAGACTTCCATCCAGTTGCCTTCTTGGGACTGATGCTGGTGACAACCACGGCCTTCCCTACTTC
    ACAAGTCCGGAGAGGAGACTTCACAGAGGATACCACTCCCAACAGACCTGTCTATACCACTTCACAAGTC
    GGAGGCTTAATTACACATGTTCTCTGGGAAATCGTGGAAATGAGAAAAGAGGTGGGTAGGCTGTGAAACT
    GATGAAGACCCAGTGTGGGCGTCCATTCATTCTCTTTGCTCTTGAATTAGAAATTCTCTGCTGGGATCTA
    GGGCCCTTAGGATTTGAAGCTAAAGGTCAGACTAGACTGTGTTCTCTCTTTCTCACCTCTTTGCTGGTTT
    TGAGTGGAGGTTGGGAAGGGGTTCCTTTCCTGTCTGGAAGATACAGAATGTGACTGCATTTCTAGAAAAT
    TCACAGTGGGCCATTCTCTGTTCCAATTTAGCTATTGCTTCTTAGGTGGGGATTCCTTTTCCCTCATTAT
    TCCTTTTTCCTTATCTCTTTGCCTTCATTTATCCCTTGAAGTCTACAAGGACCAGAAAGTGCTTTTTGGC
    TGAATGTAGTAAGTTCTGTGTGTGTGAAATCAGGATGCTCTAGGGTCAGCCCAGATAAGATGCATATTGT
    ATAGAGCCCAATAAAGTGCTAACACACACACACACACACACACACACACACAACCTAGATTAGGCTGGGC
    ATGGTGGTGGATGCCTCCCAGCACTTGGGAGGCACACGCAGGAAGATCTCTGTGAGTTAGAGGCCAGCTT
    GGTCTACATATTGAGTTCCAGGATATCCAAGACTACATTGAGAGATCCTTTCTCAAAAAAAAAAAAAAAA
    AAAAAAAAACAACAACAACAAACAAACAAACAAACAAGAAAAAAAAAACCCAACCAACCCTACCTAGATT
    AAAATATCTCCCATCTTTAAATGAAAAGTATTTTGACTCTGCTTGACAGAAAGGAAAAAAAAAATCACAA
    ACAAGATTAATAGCCTAGGTCTGGGTGTGTAGAACCAACTTTGTAATCTTGGGAAAATCCTCAACTATTT
    AGTACTCTTTATCTGCATAATTATGGAGTTACAGATAATGGCACAGACCCTTCCAGATGGAATATTCTGT
    GACTTAAATTTATTTGCCCCAAGATATCATCAAAATTTCTGTCCTTTACCAAGTTCCTACTCTATGCTGG
    GCAGTTTTTATCGTTGAATCCTTGAGACAAATGTATAAGAGGGACATCAATAATTCTTTATTGAAACATA
    GGAAATACTTAGGAGAGTAACAAGCTTAAAGCCAAGGGACTGAGCCTAAGGGTGCATTTTTATTTATTGG
    GCTGCTTAGTAGGGTTCTAAGAAGGTATAGCTTCCCTTTTCTGTGTCCTGGCTATGAAAGAGCATGACTT
    GGCCCTCTAGTGGTGCTTGTTGTAAGAGGTGTTTAAGTGATTGCTCTGGTAATCTTTTGCAGTTGTGCAA
    TGGCAATTCTGATTGTATGAACAACGATGATGCACTTGCAGAAAACAATCTGAAACTTCCAGAGATACAA
    AGAAATGATGGATGCTACCAAACTGGATATAATCAGGTAGAAACTTGTCACGTTTACTTTCAACAATTCT
    TCAAGGTTCTCTCTTGCACGTGATACCTGCATTTGTACAGTCCCAGTCAGGCAACAAAGATGGGGGGATA
    CATCCAATACCACTTGGATGTAACTGGCCTGCAGTAGCTTGAATACAAAAGGAGATTGAGAGGTACCTCA
    GCAGCTGCTAGGGTGAGGGAAAGTTTACCAACCAGTGCTATAAATGTTCACTCAATCTATGTCCATCAGG
    AGCTAAATGTTTAAAGTCTCTATTCTCATCTTGGCTTTGAGGTGTTAGATGATTAAAAAAAAAACATTTT
    TTTTGTCTAAAAAAGTGGAAGAGTAGATATAAAACATGTTATGTAAACGTCATGAGGAAAGTAACTTACA
    GATTTAATTAAAAAGCAGTTTATTTAAACAGGTAAAGGCCCACTATGCTGGGATTCTTCACCACTGTTAA
    ATGTTAGAAGTCATAGCAAGTTTAGCTAGTTCATGAGCCATCTCTTCTCTGGCTGCCCCTGGAAGGATCC
    AGGCCTACACTCCCTACTGAGAACACTCTTCACACCCCTCTCCTTCCAGCCGGTGAGATAGTCTACATCA
    GGTCTCAGGACAAGTCAGACAAGAACAAGAGTACAGGAAGGGCACTGCAGGATAGAAGGGAAGGAGTGAG
    GGTCAGAAGTGGATTCCCACTGCCACACTCTGGGGTAGGGGGTGGGGGGATATTAAGTGGTGCCTCTCTC
    TACTTGTCCAGATAATTCAGGAGGTCAGCTGATGCTGCCTGTTGTCTAATTCTGTAGAAGATACTGAACA
    GCAGGAATCTTTTTTTCAATTTTTTATTAGATATTTTCTTCATTTACATTTCAAATGCTATCCCCACAGT
    CCCCTATACCCTCCCCCCCCTCCCCGCTCCCTTACCCACCAACTCCCACTTCTTGGCCCTGGCATTCCCC
    TGTACTGGGGCATATAAAGTTTGCAAGACCAAGGGGCCTCTATTCCCAATGATGGCTGACTAGGCCATCT
    TCTGCTACATATGCAGCTAGAGATATGAGCTCTGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTAT
    AGGGTTGCAGACCCCTTCAGCTCCTTGGGTACTTTCTCTAGCTCCTCCATTGGGGGCCCTGTGTTCCATC
    CAATAGAAGACTGTGAGCATCCACTTTTTGCCAGGCATTGGCCTAGCCTCACACGAGACAGCTATATCAG
    GGTCCTTTCAGCAAAATCTTGCTGGTATATGCAATACTGTCTGGGTTTGGTGGCTGATTATGGGATGGAT
    CCCTAGGTGGGGTAGTCTCTGGATCTTCCATCCTTTTGTCTTAGCTCCAAACTTTGTCTCTGTAACTCCT
    TCCATGGGTATTTTGTTCCCCATTCTAAGGAGGAATGAAGTATCCACATGTTGGTCTTCCTTCTTCTTGA
    TTTTCTTGTGTTTTGGAAATTGTACCTTGGGTATTCTAAGTTTCTGGGCTAATATCCACTTATCAGTGAG
    TGCATATCAAGTGACTTCTTTTGTGATTGGGTTACCTCACTAAGGATCATACTCTCCAGATACATCCATT
    TGACCAAGAATTTCATAAATCCATTGTTTTTAATAGCTGAATAGTACTCCATTTGTAAATGTACCACATT
    TTCTGTATCCATTCCTCTGTTGAGGGACATCTGGGTTCTTTCCAGCTTCTGGCTATTATGAATAAGGCTG
    CTATGAACATAGTGGAGCATATGTCCTTATTACCAGTTGGAACATCTTCTGCGTATATGCCCAGAAGAGG
    TATTGCTGGATCTTCCGGTAGTACTATGTCTAATTTTTTGAGGAACAGCCAGACTGATTTCCAGAGTGGT
    TATACAAGCTTGCAATCCCAACAGCAATGGAGGAGTGTTCCTCTTTCTCCACATCCTTACCAGCATCTGC
    TGTCACCTGAATTTTTGATCTTAGCCATTCTGACTGGTGTGAGGTGGAATCTCAGGGTTGTTTTGATTTG
    CATTTCCCTGATGATTAAGGATGTTGAACATTTTTTCAAGTGCTTCTCAGCCATTCAGTATTCCTCAGTT
    GAGAATTCTTTGTTTAGCTTTGTACCCCATTTTTAATGGGGTTATTTGAATTTCTGGAGTTCAGCTTCTT
    GAGCTCTTTGTATATATTGGATATTAGTCCCCTATCAGATTTAGGATTGGTAAAAATCCTTTCCCAATCT
    GTTGGTGGCCTTTTTGTCTTATTGACAGTGTCTTTTCCCTTACAGAAGCTTTGCAATTTTATGAGGTCCC
    ATTTGTCGATTCTCGATTTTACTGTACAAGCCATTGCTGTTCTGTTCAGGAATTTTTCCCCTGTTCCCAT
    ATCTACGAGGGTTTTTTTCCCACTTTCTCCTCTATAAATTTCAGTGTCTCTGGTTTTATGTGGAGTTCTT
    TGATCCACTTAGACTTGAGCTTTGTACAAGGAGATAAGAATGGATCAATTCGGGCTGGAGAGATGGCTCA
    GTGGTTAAGAGCACTGACTGCTCTTTCAGAAGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGTTCAT
    AACCATCTATAATAAGATCTGATGTCCTTTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATGTA
    ATAAATAAATAAAACTAGATTATTTAAAAAAAAAAAAAAAGAATGGATCAATTCACACTCTTCTACATGA
    TAACCACCAGTTGAGCCCGCACCAATTGTTGAAAATGCTCTCTTTTTCCCACTGGATGGTTTTAGCTCCC
    TTGTCAAAGATCAAGTGACCATAAGTGTGTGGGTTCATTTCTGTGTCTTCAATTGTGTTCCATTGATCTA
    CCTGTCTGTTGCTGTATCAATACCATGCAGTTTTTTTTATCACAATTGCTCTGTAGTACAGCTTGAGGTC
    AGGCATGGTGAGTCCACCAGAGGTTCTTTTATTGTTGAGAATAGTTTTTGCTATCCTAGTTTTTTTTTTT
    TTTTTTTTTTGAACAGCAGGAATCTTATTCTCAAATTGAATCTATTCCTAGAAAGAACTGACTTCCTTTT
    CCATTTTACTTATAGGAAATTTGCCTATTGAAAATTTCCTCTGGTCTTCTGGAGTACCATAGCTACCTGG
    AGTACATGAAGAACAACTTAAAAGATAACAAGAAAGACAAAGCCAGAGTCCTTCAGAGAGATACAGAAAC
    TCTAATTCATATCTTCAACCAAGAGGTGAGTGCTTCCCCATCTCTCATGCAGTGTGGGAAAGAGGACACC
    CGGCACCTCAGGGTAGCGGCACTTTTTCCAGACAGCTGCTCAGAAGGGAGAGAGTCTGAACAACAGTTCT
    TGATTATAAGGCCTTGCTTTGTTTTACTTTGGGGTTTTGTTTGAGGTTCTCTTTTGCAAAGAACATCAAT
    ACCTGCTTTAAACTGTATTAATAGAATGTTACTAATTGCGTAAGAGGTATGAAAACTATGACAGCCATAC
    ATAGTCACCCATTATGAGAGCACAGAGACAAAAGTGACTTTAATATTTAATCCTTGGCAAGTGACATTTT
    TGTAACCAGAGTTCTAATGCAGAGAAGTAGCCAAAAGCTAAAATGTCAGGGAAGGAAGGGATCAAGGGCT
    TCTGGCTACCATTAGCCAGAAGAATGGTAGATATGGAGGATTAATGACTCAGAGTGTGGGCGAACAAAGC
    CAGATGCAATAAGAAGGGCCTGGAATGAAACCCTCTTGCTAAGGCTGCTTTTGCCACTTGTAGTTTCTTG
    CCTTAAACCAGAGAGTTTCCCAATTTAATGTGCACAGGAACCACTTAGGGTCTTGTTCCAGCAGGGTCTT
    ACTTAGGAGGTCTAGGAAGGGAACTAAGATTCATTTTTGTAAACAGCTGCTTGTTTATGACCCTGCTATA
    TAGTATTCAGGCTCCTAAGTAGTGGGGCAAGCCTTCCAGTTAGTCTTCCCCATGCCAGTGGGCCCATGGA
    AGGGTGTTTCCAGACTTCTTCATGCTACCACACTAAGAGACTCTCAACAGAGTCTGAATGGAAACCACGA
    AGGAACACATTTGTTTTAGATTCCTCTGTGCCACCTTTACTGATGGGAGCTTCTGTTTTCCAGTAGAATA
    CAGTATGTTAGCATGGATGCTTGGATAACAGACAAATAAGATGGTGGTGCTGACCTCTGGACGCTTACTC
    TCTAGTGGCAGACAGAACAGTAAGGTTAGAATTCTGTTGCTATTAAAAAACTAATAATAATCACCTTGAA
    AAAGAATGGAGTTGTTAGGCATGGGTCTCTCTCGAGTAAGCTTGGAACAAAAGCATCTCCCCTGGCTTGG
    GTGAGTCAAAGCAGATGGACTTAGCTCGTCTCATTCATTCTAAATTAGAACTTCTTCCCACAGCCCAGAA
    CACGCCACAAGAAAAAAAAAATGTGCAATATTTAACCAGTCTTTGTTTTTTCCTCCTTTAGGTAAAAGAT
    TTACATAAAATAGTCCTTCCTACCCCAATTTCCAATGCTCTCCTAACAGATAAGCTGGAGTCACAGAAGG
    AGTGGCTAAGGACCAAGACCATCCAATTCATCTTGAAATCACTTGAAGAATTTCTAAAAGTCACTTTGAG
    ATCTACTCGGCAAACCTAGTGCGTTATGCCTAAGCATATCAGTTTGTGGACATTCCTCACTGTGGTCAGA
    AAATATATCCTGTTGTCAGGTATCTGACTTATGTTGTTCTCTACGAAGAACTGACAATATGAATGTTGGG
    ACACTATTTTAATTATTTTTAATTTATTGATAATTTAAATAAGTAAACTTTAAGTTAATTTATGATTGAT
    ATTTATTATTTTTATGAAGTGTCACTTGAAATGTTATATGTTATAGTTTTGAAATGATAACCTAAAAATC
    TATTTGATATAAATATTCTGTTACCTAGCCAGATGGTTTCTTGGAATGTATAAGTTTACCTCAATGAATT
    GCTAATTTAAATATGTTTTTAAAGAAATCTTTGTGATGTATTTTTATAATGTTTAGACTGTCTTCAAACA
    AATAAATTATATTATATT
  • Protein Sequence : Show Sequence
    >gi|13624311|ref|NP_112445.1| interleukin-6 precursor [Mus musculus]
    MKFLSARDFHPVAFLGLMLVTTTAFPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELC
    NGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKAR
    VLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQ
    T
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
21. IL10
  • Gene Name : IL10
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 3586
  • NCBI Protein GI : 10835141
  • Locus Tag : RP11-262N9.1
  • Genbank Accession : AF295024
  • Protein Accession : NP_000563
  • Taxonomy ID : 9606
  • Chromosome No : 1
  • Gene Starting Position : 206940947
  • Gene Ending Position : 206945838
  • Gene Strand (Orientation) : -
  • Protein Name : interleukin 10
  • Protein pI : 8.12
  • Protein Weight : 19821.37
  • Protein Length : 178
  • Protein Note : Also known as CSIF; TGIF; GVHDS; IL-10; IL10A
  • DNA Sequence : Show Sequence
    >gi|224589800:206940947-206945838 Homo sapiens chromosome 1, GRCh37.p5 Primary Assembly
    TGATGTGAATAAGATACATTTATTTATTCAAAATTAAAATCTGCTATGAAGACAGACAAACAATGTAACA
    TTCCCAGAGGAATTGAATAAATTCTAGTTAAAACTGAGTTCTATTAGAACCAAATTTATTTTTATTTTTA
    TTTTTTGAGACAGAGTCTTGCTCTGTCACCCAGGCTGGAGTACAGGGGCATGATATCAGCTCACTGCAAC
    TTCCATCTCCTGGGTTCAAGCAATTCTCTTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGCGCGCC
    ACCATGCCCGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGACCAGGCTGGTTAGGA
    ACTCCTGACCTCAAGTGATCCACCCGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGC
    GCCCGGCCTAGAACCAAATTTAGGTTGTTATAAATAACAAGCTGGCCACAGCTTTCAAGAATGAAGTGGT
    TGGGGAATGAGGTTAGGGGAATCCCTCCGAGACACTGGAAGGTGAATTAATCATCAAAGGGGCTCCCTGG
    TTTCTCTTCCTAAGAGTATTTGTAGCAGTTAGGAAGCCCCAAGCCCAGAGACAAGATAAATTAGAGGGAG
    GTCAGGGAAAACAGCTCAACAGCTAGAAAGCGTGGTCAGGCTTGGAATGGAAGCTTCTGTTGGCTCCCCA
    AAGAACATTTTTTTTCCTCCCTTATGTAACTTATAATTTATCTTAAAACACTCAAATACCATAGTGTGTC
    ACCCTATGGAAACAGCTTAAAAACAGGTGAAAATAATAAATATTGAAAAAAATTATAATATTGGGCTTCT
    TTCTAAATCGTTCACAGAGAAGCTCAGTAAATAAATAGAAATGGGGGTTGAGGTATCAGAGGTAATAAAT
    ATTCTATAAGAGAGGTACAATAAGGTTTCTCAAGGGGCTGGGTCAGCTATCCCAGAGCCCCAGATCCGAT
    TTTGGAGACCTCTAATTTATGTCCTAGAGTCTATAGAGTCGCCACCCTGATGTCTCAGTTTCGTATCTTC
    ATTGTCATGTAGGCTTCTATGTAGTTGATGAAGATGTCAAACTCACTCATGGCTTTGTAGATGCCTTTCT
    CTTGGAGCTGTGCAGAGAGATAAGAAACATACAGTTAAAATCCTTTCTGGGGACTAAATAGGCTCCCCTC
    CCTCATGCTCATGGATGGGCATGACCTTGAGTGCAGAGGTTGCTTGTTCTCCCTCACGCTGGGAAGTAAA
    CCAGAGGATAGGCCTAGCTGCCTTGGGAAAGGCTGTGCATTGACCTTCATCTCCTCCAGGCCTCCCCCTA
    GCAAGGGGAGCATTCATTTTGCAAAGCACTGTCTTCAAAATGATGCCTTTTCAACAATTTTAAAGGTCAC
    GTATTCACATGATGATTATGCAGAGTTTGATGAAAAGACATTAGAGGAAGCGCTTCGAAAGCAAGACGGT
    GAGAGGAGAGGAGGGAGCCTTTTAAATGTCTGTGTTCTCCCACCAACACACTGAGCAGGCCCTGCCCTGC
    CGCCCTGACCTACTATGAGAAATTCACACCCCCTGATGTTGGCACTCTGTGCAGTGTCTGTCCCCAGCCC
    ACGAGGAGGACATAGGCCGCACGGTTTCTGGGAAATCAGACTGAAAAAGTTCACGCACAGTTGGAAGTGT
    GAAACCCCATTTCGTTTGGGAACTGACATTCTCAGGCACCTCCGCAGGTGCGAAGGGCAGATGGAAAGCG
    GGCTTGAACCAACTCCAGGTTAAGTGAGAATCCAGCTTCCTGCTCATTCTGAGTTCTGGGGAGTCTGGGC
    TGAACCCCCAGGTGTATTTGTATGTGCACATTTATGTGCCAGCTTCCTGCACGTGTGGGTTCAGCCTAGA
    TTCCCAACAAGCTACTTTCTGTACTTTCTGTTTCCTTTGTCACTCTCCCCAGCATGCAGCTCACTGTGTT
    CACAGGCTGGCCGGCCAGCCTAACCCGCAAGCCTGCAAAGGCAGCGAGCAGTCATTTAGAAACCCCCAAA
    GACACTTAACAGAAAACAAATACAATCAAGGCAGCAGCTCCTCACTGGCTGAGCAGGTCATACCATCTGT
    CAGGTTCCCACACTCTCTCCAATGAAGAATGGGGCCTATTGAGTCCCCACCACCTTCCATGCTTTGGGGT
    TGGGGAGTGGGCATGGGTTGTGCTCTGCAGACCCTCTCTGCCACCATCCAAGCTCACCTTATTAAAGGCA
    TTCTTCACCTGCTCCACGGCCTTGCTCTTGTTTTCACAGGGAAGAAATCGATGCTGTGGAAGAAAAGAGA
    AAGTGTTGGTGATCCTGGCTTCCAGCTCCATGTCCATCACACTTAGGGGACAAGCTGGTGGCATCATGAG
    GAGGCCAGATTTATCCAAATGCCTTGCCTCACAGCTCCCAGAGAGAACTGAGCCCTGCTTCCATGGCCCT
    GGGAGTTAAATGTATTTCTCCAGGACTCACTCCAAGATGTTTGCAAGGCTTGGATGACAACCCAGGTCTT
    GGGATTCCTTCCTCCCCCAGAACTCTACAGTATCTCCTGCAGTGCTGAGCGAGGCATTCTTCAGCCTGTC
    CTTTTCTGCCTCTCTCTCCTGTTCCTCCCCGTCAGTCACAGCCACCGAGGAACACTTCTAAGCATCTGAG
    GAGGGAAGCTACTGGGACCAAACCCTTCACATAGCCTTGTCCTACAAATAGACACCAACTGATTTTCCAA
    TATTGGTGACAGAACAGGGGAAACCATTAGTAGTGAGGGCATCATTTCAGTTGGAGAAATAAATTACCTG
    GAGGAATTACTCATAGACACTAGCCTGGGGATGAATAGCCCCCTTGTCCCTTCTAAAATGCCAGGGGAAA
    TCCTGTCAGTCAATTGGGCCCTTCTTAGAGCTTTGCTTTCAAAACATTGAGATTAAAAGAGGTGAATGAT
    GTTTCCTCTGCTTGGGAACCTTAGCTCCCCGCCCAGGGTCTAGTTACAAAGAGGCAGTGGAGGCCTGACT
    GAAGCTCTGGGCTCCTTTTATGAAGAGAGAGGGGAGTAGTTAATAACCCACAAATGACTCACAAATAACA
    GGAAAGCTGTTTGCAAATTTCACATGTAAATGCCTAGCAGCCACCCCCAACGCCTGCTCAAAGAGAAATG
    AGCAAGAGATCTGACTCCAGGAGTCTTTCCTCATTTACAGCTAGCTCTGCCAGTCTGTGTCTTTGCTGTG
    TCTGTGGATGTGAGTGTCCCTGCTGGTCTGTAGGAGATGGTATTTTGGGGGCAGCTGCAAGGGAAAAAAC
    TGATCTGCTACTTACACAGCGCCGTAGCCTCAGCCTGAGGGTCTTCAGGTTCTCCCCCAGGGAGTTCACA
    TGCGCCTTGATGTCTGGGTCTTGGTTCTCAGCTTGGGGCATCACCTCCTCCAGGTAAAACTGGATCATCT
    CAGACAAGGCTTGGCAACCCAGGTAACCCTAAGGGCAGGAGCCAAAGGTGAGTGAGAGATTGGCGGAGGT
    GGCTGGGAGGAGGGGCTGCTGCAACTAGCTTAAGAGGACAGCTTGGTTCTAGCGATCCTCCTTCACCAGA
    AGCCTCCCCGAAGGGACTGAGCCCTTTGTAAACCCTCTGGCTGCTGGATGTGCTGAGTTAACATCTTCCC
    ACTTCTCCTTTTCAAAGCGAAGGAAACAAACCCAATTCCCTGCAATCAGGAAGCAGAGTCTCCCTTCCCT
    TAATCATGCTGCACACTCCCCCAGCACCCCGCCCCTGCTCTCACCTTAAAGTCCTCCAGCAAGGACTCCT
    TTAACAACAAGTTGTCCAGCTGATCCTTCATTTGCTGCAGGAAGAACAAAAGGAGAATGAACTTGAGGTT
    TGGGGAAATAACTGAAATGCGGTCTTTTTGATGCCCTTTCATTTAGAGATTTTTTTTTTTAAATAAAATT
    GGCTCTGGCCCAAAAAAATCAAAAGGGGAGTTTTAAAAACAGAGGATTCAACAGTGATGGGACCATCACT
    TAAATCAGGTCCTCCTCCTCAGAGTCAAGTTATTTAAAAAATCTGGCCGATTTTGAAGGGAAAGCAGAAG
    CTTGACTCAGTCCTGGTCTTCTTTCTGCGGAGCTACATTCGGCTTTAAGGGACACCTATGTCAACCCTTC
    GGGGCCTTGAGCCCCCCTCATGTCCTGTTCTCTGCTTTTCTTTCTCCCCACTGTAGACATCCAGTTTAGC
    TTGGAAAGATCCCAGGGATTAAGAAGCTCCTTCTAATGCAGGTTTCCCTCATGTAGAGTGCTTCCCTAAA
    CCACTGGTCTTTCAGGTCTGGAAACGCTCTAAGCAGAGGCAATTTAAATTTCCCTGCTGCAAGGCATGGG
    GAGCATCTTCACCTCGAATCAGACATTTCTTTACTTCTCCTAGCCAATAAAGTATAGAGCGCCAGCAGGA
    TCTTATAAGTTTCTCAGCACGAGAGAGAACGCATTTACTTTATAAGGCAGGTTTCCTGCACATTTACTGT
    ATCATCTGGCTTAGCTTAACTCACCAAACCCATGGCTTGATCTAGGATTCTCTTAAGAATTTAAGTTTGG
    GGGAATAGGTGTTGGGGATGGAGGTGGAGGCGCAGGAGGAGGGTTCTTATAGTTCCAGGAGAATGTCTAG
    TTCAGGCAGTCCCAGGAAGAGAGAAAGGACAGGAAGGAATCATACTCACAAAGAAAGTCTTCACTCTGCT
    GAAGGCATCTCGGAGATCTCGAAGCATGTTAGGCAGGTTGCCTGGGAAGTGGGTGCAGCTGTTCTCAGAC
    TGGGTGCCCTGGCCTGGGCTGGCCCTCACCCCAGTCAGGAGGACCAGGCAACAGAGCAGTGCTGAGCTGT
    GCATGCCTTCTTTTGCAAGTCTGTCTTGTGGTTTGGTTTTGCAAGAGCAAGCCCCTGATGTG
  • Protein Sequence : Show Sequence
    >gi|10835141|ref|NP_000563.1| interleukin-10 precursor [Homo sapiens]
    MHSSALLCCLVLLTGVRASPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESL
    LEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVE
    QVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
22. IL10
  • Gene Name : IL10
  • Sequence Strain (Species/Organism) : Bos taurus
  • NCBI Gene ID : 281246
  • NCBI Protein GI : 41386772
  • Locus Tag : BOS_15514
  • Genbank Accession : GJ062448
  • Protein Accession : NP_776513
  • Taxonomy ID : 9913
  • Chromosome No : 16
  • Gene Starting Position : 4402473
  • Gene Ending Position : 4406411
  • Gene Strand (Orientation) : -
  • Protein Name : interleukin 10
  • Protein pI : 8.14
  • Protein Weight : 19616.38
  • Protein Length : 178
  • DNA Sequence : Show Sequence
    >gi|258513351:4402473-4406411 Bos taurus breed Hereford chromosome 16, Bos_taurus_UMD_3.1, whole genome shotgun sequence
    AAATAGCTCACAGAGAAGCTCAGTAAATAAATATATGTGGGAGCTGAGGTATCAGAGGTAATAAATACTC
    TAGGGGAGAGGCACAGTACAGGTCTCCAGGGAGCTGGCTCTGCTCTCCCAGAACCCTGGATTGGATTTCA
    GAGGTCTTCCGTTTACGTCATGGAGTCTAGTAGAGTCACCATCCTGGAGGTCTTCTTCCCTAGAATGCTT
    CACTTTTGCATCTTCGTTGTCATGTAGGTTTCTATGTAGTTGATGAAGATGTCAAACTCACTCATGGCTT
    TGTAGACACCCCTCTCTTGGAGCTGTGTGGGGAGACAAGACGTGTTCAGTGAAAACTCCCTCTGAGCAAG
    TGAGTGGGTCCCTGCTGGGAAGGTCATGACCCTGGGCCCAGAGGCTGCACTAGCTTGCTTGCATTGGGAG
    ATGAAGGACAGACTCTGGGCATCTGGAGAGATGCCGGACTGTCAGCAAAGGCTATGCGTTGCCCTGGACC
    TTGCCCGGTGGGCAGCAAGAGGAACATTCACTTTGCAAAGTGTGGTTTTTCGAACGGTGCTTTTCCAGCA
    ATTTTAAACGTCATGTGCTCACGTGACGCTTATTCAATATCTGATGAAAACACATTAGCGGAACCACTTT
    GAAAGAAAGACAATGAGAGAGAGAGAGAGAGGTAGCCTTGTTCCTGTTTATGTTCCCCCACCCGTGGAGT
    GAGCAGGCCCAGCCCCGCTGCTCTGACCTACTGCAGGGAGGTTCATGGCCCCTGATACTGGAACCTGCCC
    AGGGTCTGTCCCCCACCCACGAGGAGGACATAGGTTCCGCGGCTTCTGGAAAATGGGGTAGAAGAGCTCA
    TGGACAGCTGGAAGTGAGAAAGGCCACTTCGTTTGAGAGCTGAGATTCTCAGGTGCCTTGTGTGGTGAGG
    CTGGCGGGTGGAAAGAGAGGGGTCAAGTCAGGTAAGGAGAGCTCTGCTTCCTGCTCTGGGCTCGGGGTGC
    TCAGGCTGCGCCCCTGGGTGTGTTTGTAAGTGCACGTTCACGTGTCAGCTTCCTGCACGTGCGGCTTCAG
    ACTGGATCCCAACAAGCTACTTTCTGTTTCCTCTGTCACTCTCCCCAGCACCCCGCTCGCTGTGTTCACA
    GCTGGCGGGCCAGCCCAACCGGCAGGCCTGCGAAGGCAGGGAGCAGTCATTTAGAAACCCCCAAAGACAC
    TTCACAGAAAACAAATATAACTGAGGTGGCGGCCCCTCACTGGATGAAGAGGTCACCCCACTTGCCGGGT
    TCCCACACTCCCTCCGAGCAAAGTTCAGGGCCTGCTGAGCACCTGCCCACCTCTGCCCCTTCGAGAGTGG
    ATGGAGGTCACGGTCCTCAGGCCCTCCCTGCCAGCAGCCCAGCCTCACCTCACTGAAGACTCTCTTCACC
    TTCTCCACCGCCTTGCTCTTGTTTTCGCAGGGCAGAAAGCGATGCTGTGGAAAAGGAGAGAGTATGAGCA
    ACATTGGTGGCTGGTCCCAGATGCACCGTGCTTGGGAGACAGCCTGGGAGCAGGAGGAGGCCAGCTCATC
    TTGAAGGCCCACCCTGCATCTCAGGGAGGACTGAGCCTGGGCACCCTTCCCCACCAGGCAGAGAGGAAGC
    ACAGCAAGGCAGGGGCCAGGCGGCATGTGTGTCTCTAGGAGCTCCTCCCCACCGTTAACAAGGCTGCATC
    AACACCTCTGTCTTGGAGTTTCCTTCCGGGCGCATCCCAGTGCCAACCAGACTGCTGAGCCAGCCCTTCT
    TCCATCCTGTCCTGCTTCTGGCCTGTCCCCCTCCTTGGTCCCAGCCACAAGGACCATTTCTAAGTGCCCC
    GCAGAGCAGAGACACAGGGTCAGCCCTGCCAGCCAACCCCCTTGCCTAGCGCTGTCCTGCAAATACAGCC
    CAACTGATTTCTCAACATCGGCGAGGGAGCAGAGGAAACTGGTGGGGAGGGTATCATTGCAGTTGGAGGG
    ATCAGTAATCCTGGGGGATTATTCGCAGACACTAGCCTGGGACCAAGGATCCCCCTGGCTCTTCTAAAAT
    GCCAGGACAGTCCTGGCAGTCAGCCGGGCCCCACCTGAAGCTCTGCTTTCAAAAGGTCGAGAAGAAGAGG
    AAGGGGCCTTCCCTCTCCCAGGCCCTCTGCTTCTGCCTGCTTCCGTGGGGCCCAGGGCCTCTGTCCCCAG
    GGACAAGGGAGCCCTGGCTGAAGCTCTGGGCCCCTTTCACGTCGAGGCCGGGGACTGCTAATAACCCACA
    AATGACTCACAAATAACAGGAAAGCTATTGCAAATCTTCACATGTAAGCACTCAGCAACCACCCCCACCC
    GCGGCTCAGAAACAGCCGAGCCAGGCCTGGGGTTCCGGAGGGGCAGGGGCCCCGAGGGCTGGTGTCCCGG
    GGTCTCTGGGCGAGAGCAGAGCTTGAAGAGGAGGTCTCAGCACGAGCCCAGAGGAGCCAGAGCTGGCCTG
    CTACTCACACAGCGCCGCAGCCGCAGCCGGAGGGTCTTCAGCTTCTCCCCCAGTGAGTTCACGTGTTCCT
    TGATGTCAGGCCCGTGGTTCTCAGCCTGTGGCATCACCTCTTCCAGGTAAAACTGGATCATTTCCGACAA
    GGCTTGGCAACCCAGGTAACCCTGAGGGCGGAAACCAAGGCGGGTGGTGACCCGGGAGGAAGGGCTACAC
    TGCCTGAGAGGACGGCTCTGTTCCCCAAGGACCTTGTTCAGTGGCCACCTCCCCCTGGAACGGAGCCCTT
    GGCAACACCCAACACCCTGCCTCCCTCTGGCTATGTGCTGAGTTAAGATCTTCCCACTTCTCCTTTTTTT
    GAAGTGAAGGAAGCAGACCCAGCTCCCTGCAGGCGAAGAAGCAGAGTCTCCCTTGGGCACACCGCAAGCT
    CCTTGCCCCCGCCAAACGCACTCACCCCAGCCCTCACCTTAAAGTCATCCAGCAGAGACTGGGTCAACAG
    TAAGCTGTGCAGTTGGTCCTTCATTTGCTGCAAGAAGAACAGAAGGAGAATGAACCCAAGGTTTGGAGCA
    ATACTGAGAAGGACTGTCTTTCTGATATCCTTTTATTGAGAGACTTTTTGATTTTTAGTCAACACAGCCC
    TGTCTCCAAAAGGGAAAGGAGAGGTTTTGAAACAGGACATTCAGCAGCGCTGAAGCCCTTAGTTAAACCA
    GGTCCTTCCTCCGGGGATCAAGTTCTTTTAAAAACCTGGCTGATTTTGAAGGCAAGGCAGAAGCCTGCCT
    GGGTCCCAGTCTTCTTTCTGTAGAGCTGCGTCACGTGTGCAGGGACCCCCATTCCCAACCCCTCAGCACC
    CTCTGCTTTGCTGTCTCCAGCTGCAGCACTGACCGTTCTCTGGGAGTGGCAAGCATCTTCTCCTGCAGGT
    CTCCCCCTGTATGCGCTACCTCCAACCCATTTGTCATTCAGGTTCTGGAAGCCCTCCAAGCTGAGGCAAC
    TTTTGTATCCCTGCCACGGGGTTTTGGGAACATCTTCACCTCCAATCAGATCTCTCCCAGCCAATGAGGT
    ATTGAGCTCCAGAGGAATCCTTTACAGATTTTCTAGCATAGGCTAGATGTGCCTTTACATTTTTAAAGAC
    AACTTTTCTTGCACAATCTGCTACATCACCAGAGCTCAGATTAACTCACTGAACCCACAGTTTGACCAGG
    GACTCTTAAGAATTTCAGCTTCGAGGGGAATGGGTGCTGAGGGTGGAGGTGAGTGAAAAGGAGGCTCTTG
    TGAGTGCCTGCAGAATGGCTCTTCCTGCGGTCCTAAGGGAGAAATGAAGGACAGGAGGGCTTTATACTCA
    CAAAGAAAGTCTTCACCTTGCCGAAGGCAGCTCGGAGCTCCCGCAGCATGTGGGGCAGGCTGGTTGGCAA
    GTGGATACAGCTGCTGTCAGACAGGGTGCTCGCATCTCGGCTGGCTGCCACCCCAGCCAGGAAGACCAGG
    CAACAGAGCAGGGCTGAGC
  • Protein Sequence : Show Sequence
    >gi|41386772|ref|NP_776513.1| interleukin-10 precursor [Bos taurus]
    MHSSALLCCLVFLAGVAASRDASTLSDSSCIHLPTSLPHMLRELRAAFGEAKTFFQMKDQLHSLLLTQSL
    LDDFKGYLGCQALSEMIQFYLEEVMPQAENHGPDIKEHVNSLGEKLKTLRLRLRRCHRFLPCENKSKAVE
    KVKRVFSELQERGVYKAMSEFDIFINYIETYMTTKMQK
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
23. Il10 (interleukin 10)
  • Gene Name : Il10 (interleukin 10)
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16153
  • NCBI Protein GI : 6754318
  • Locus Tag : AL513351.1
  • Genbank Accession : NM_010548
  • Protein Accession : NM_010548
  • Other Database IDs : MGI:96537; Ensembl:ENSMUSG00000016529;
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • DNA Sequence : Show Sequence
    >gi|6754317|ref|NM_010548.1| Mus musculus interleukin 10 (Il10), mRNA
    GGGGGGGGGGATTTAGAGACTTGCTCTTGCACTACCAAAGCCACAAAGCAGCCTTGCAGAAAAGAGAGCT
    CCATCATGCCTGGCTCAGCACTGCTATGCTGCCTGCTCTTACTGACTGGCATGAGGATCAGCAGGGGCCA
    GTACAGCCGGGAAGACAATAACTGCACCCACTTCCCAGTCGGCCAGAGCCACATGCTCCTAGAGCTGCGG
    ACTGCCTTCAGCCAGGTGAAGACTTTCTTTCAAACAAAGGACCAGCTGGACAACATACTGCTAACCGACT
    CCTTAATGCAGGACTTTAAGGGTTACTTGGGTTGCCAAGCCTTATCGGAAATGATCCAGTTTTACCTGGT
    AGAAGTGATGCCCCAGGCAGAGAAGCATGGCCCAGAAATCAAGGAGCATTTGAATTCCCTGGGTGAGAAG
    CTGAAGACCCTCAGGATGCGGCTGAGGCGCTGTCATCGATTTCTCCCCTGTGAAAATAAGAGCAAGGCAG
    TGGAGCAGGTGAAGAGTGATTTTAATAAGCTCCAAGACCAAGGTGTCTACAAGGCCATGAATGAATTTGA
    CATCTTCATCAACTGCATAGAAGCATACATGATGATCAAAATGAAAAGCTAAAACACCTGCAGTGTGTAT
    TGAGTCTGCTGGACTCCAGGACCTAGACAGAGCTCTCTAAATCTGATCCAGGGATCTTAGCTAACGGAAA
    CAACTCCTTGGAAAACCTCGTTTGTACCTCTCTCCGAAATATTTATTACCTCTGATACCTCAGTTCCCAT
    TCTATTTATTCACTGAGCTTCTCTGTGAACTATTTAGAAAGAAGCCCAATATTATAATTTTACAGTATTT
    ATTATTTTTAACCTGTGTTTAAGCTGTTTCCATTGGGGACACTTTATAGTATTTAAAGGGAGATTATATT
    ATATGATGGGAGGGGTTCTTCCTTGGGAAGCAATTGAAGCTTCTATTCTAAGGCTGGCCACACTTGAGAG
    CTGCAGGGCCCTTTGCTATGGTGTCCTTTCAATTGCTCTCATCCCTGAGTTCAGAGCTCCTAAGAGAGTT
    GTGAAGAAACTCATGGGTCTTGGGAAGAGAAACCAGGGAGATCCTTTGATGATCATTCCTGCAGCAGCTC
    AGAGGGTTCCCCTACTGTCATCCCCCAGCCGCTTCATCCCTGAAAACTGTGGCCAGTTTGTTATTTATAA
    CCACCTAAAATTAGTTCTAATAGAACTCATTTTTAACTAGAAGTAATGCAATTCCTCTGGGAATGGTGTA
    TTGTTTGTCTGCCTTTGTAGCAGCATCTAATTTTGAATAAATGGATCTTATTCG
  • Protein Sequence : Show Sequence
    >gi|6754318|ref|NP_034678.1| interleukin 10 [Mus musculus]
    MPGSALLCCLLLLTGMRISRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSL
    MQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVE
    QVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS
  • Molecule Role Annotation : IL-10 plays an important role in Th2 type immune response.
  • Related Vaccine(s): M. tuberculosis DNA vaccine pAK4-sod , Mycobacterium tuberculosis sigE mutant vaccine
24. Il12b
  • Gene Name : Il12b
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16160
  • NCBI Protein GI : 6680397
  • Locus Tag : RP23-388G23.1
  • Genbank Accession : AL607030
  • Protein Accession : NP_032378
  • Taxonomy ID : 10090
  • Chromosome No : 11
  • Gene Starting Position : 44400062
  • Gene Ending Position : 44414016
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 12b
  • Protein pI : 6.5
  • Protein Weight : 35952.67
  • Protein Length : 335
  • Protein Note : Also known as p40; Il-12b; Il12p40; Il-12p40
  • DNA Sequence : Show Sequence
    >gi|372099099:44400062-44414016 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
    CAGAAGGAACAGTGGGTGTCCAGGCACATCAGACCAGGCAGCTCGCAGCAAAGCAAGGTAAGTTCTCTCC
    TCTTCCCTGTCGCTAACTCCCTGCATCTAGAGGCTGTCCAGATTCAGACTCCAGGGGACAGGCTACCCCT
    GAACCAGGCAGCGTGGGAGTGGGGTAAGTGGATTCTGGGAGCATCTCGGATGGCTTTCCCCGCTGGTGGA
    AACCAGGGGCTTTGACGACAGGGGCTTCAAATAGTCATTTAAAAAGTCAGCTAATTACAGATGTGATGGC
    GCACACCTGTAATCTCCAAATCTGGGAGGCAGGAAACAGAATGCACAGGAGTTTAAGGCCAGATTCAGCT
    GTGTAATGAGTTTGAGAGAGTCTCAAAAAATTGAGCTACTAATAATATGTGCCAAGCATTGTGGACAGAT
    GTGTATTTGATAGAAAGGTTTGCTTTGTCTATATAGACACATATACTATGTGCATTTTCTTAAGTATTAA
    TGCATATGTGTTAAGGAGTGTTGTTAAAATTCTGTTCTTGCCTTAAGAGTGAAACTTAAAAAAAAAAAAA
    GCCTTTCATGTACCCCTTCAAGCCTAAGCTCATCACTGAATGTCCTACCTGTGATGGCCTTTAGAATCAG
    CTATGATAATCAAAGTGGAGAGCTGGGTTTGGGGGTTCCTTTGGATTTTAACTAACTTTGCCACCTCTTC
    AAAACTGGAAAGCCCAAGGCATGTTCCAAACTATTTCCTAAAGGGTTCTAAAATCTGTGAGAAAGGGAAG
    GCTAATCTTCATGGGTTGGCTTCCTCTGCTTGCCCAGGTGAAATCATGAGCACCAGATGGGGCAGGGTGA
    TGGGGCAGGGTGACATCTTGCTTTCACCCAGGGATCACTGAGCCTCAGCTGGGGGCTCCGTTTGTTCAAG
    CAAGGCATTTCAAGATGAAGTTAGGTCACCCCCAAGAATTCATTTTGCTGAGATCTGAAATACTGTGATT
    ATTGCACCAACAATCCATGGACTCTCTCTAGGCTAGATTCTGGATATAAGACTAGACAGATATGACCTCT
    GTCACACACCTCGGTATGGGAAAGGGGGGACAGACATTAAAGACGTAATCAAATGATTGTCATTTCAGCG
    ATGCTGAAGGGGAAGAGTAGCTATTTGGGGTCCTGTTTTCATTTGGGGCTCAAGCTGGCACTTTCTGTGG
    ATCATTTCATGAACTCCTAACCTGACAGAGTTATTTTTAATCTAGGAGTACAGGCTTGACAAAGTCAACA
    CAACTTGTCAAGGTCACATAACTAACAAGTGGCAGACTGAGGTCTGTTTGGCTGTAGAACCAAGGCCCTT
    TGCCCGTGCCCTCCTTGCCATCCCTGACAGCCACGCCATCTGTACTGTGCATTGTTGTTTCTGGGCATTT
    TTATTTTTACATATCATTTCTCTTTTAAATTAAAGAATAACAGAATGAAACTTGCAAAAGGGATGTCCAC
    TCAGGCACCCGCTGGCAGCAATGTTTGGAAGCATTTGCATGCTCTAGGGAGCTAGAGTATCCAGCCCAGG
    GTTCCTGTGCTCTTTGCACAAGACTTTCAGGACTTAAGAAAACTAATGAAAACAAACAAACAAACAAACA
    AACAAAAAAGAATTGTTAAAACATAGCATATGGAAGAATTGTGGAGTGAGAGGGAAACAAAGCCCAGCAT
    CTGCCAATCAGCCTGAAAGCCAAAGCCTGGCTACATACTGCACACTTTTTTGCTTGTGCAGATTTGATTA
    AGAGGCGACCTGCCATCATCTCCACCCTCTGCTGGTCTTCTATGATCTAAGATGTAGGTGATGCCGAAAT
    GAAGATTGTTCCTTGGACCATTGCCAGGGCTCCCTTCAGCCTGAAATATGGTTCACGTGTGATGCTCAAG
    TGCATCTGAGGCTGACAGAGGAAATGCTTGGCATGTAGCCAAAGGGGAAATCCCTGTGTTGAGAGCATAT
    GAAGAGATCACCAGGAGATGCCAGGAATCTCTGCCTTTTTTCCCTGCCCAGAGTCCTACCATCTGGGTTT
    CACAGCCGTGTATAATTGCATCACCTTCTCTGGGCATTTCTCTCTACAGCCTGGCAGATTTTTATTTTTC
    AACCCAGTGTGCACAAAACAGAAAGAATTTCAATTTTTTTCCTTAGCTTTTTACTAGTGCAAGGTATTAT
    ACAATGTCATCTCACTGCATGCCCAATAGCTCTTGAAGATCACATAAGCCTTATGTCTTTTTATATGAAG
    GCAGTCGGGCTCAGAGGAACTAAACCAACACCAAACAGCTACTGAGTAGTAGGGCCAGAATCCAAGTCCA
    TGTCTGCTTGACTCCAAGCCTATGAATGTAGCAACCAGCACCATTTCAGCTTGTACTTGTCTTGACTATA
    TGATAATTTGGACCTAGACATGTCCTGACTCCTAGTCCTGTCTCTGTCATGAAAAAGAAATCATAGCAGG
    GCCCTCAAGAGGTTAGCAGTACTTCTGTTCTCTCAAAGGTCGTGATTTCTGACCAAGGCCTGTTTATTTT
    TAAGGCATTTGCCTATAACATTTAAGCCTTAATTCATATGGGTTAGACTGTGGTCTCTCAAATCTTGTTC
    CTGACGGGCGGTGGCACCCTCCAGGATAGATGAAAAGTATTTCTGAAGGGAATTAGTAATCTACACTGAT
    TATCTCAACCACATTTATGAGTGAGGTATAATCTTTACAGTCTAAAGGTTTGTGTTGCCCTGGTGTTAAT
    CTAGACTGGGTTACAATACAGGATGGTTACTGACTTATCTATTTCATGATAGGTTAATCAATATGGTACA
    GTCTTCTGCCAAAGAACACCTTGAATTTTAGGAGTTCTGGACTCTCAAACCTAGCTAGCACATTTGCCCT
    CACAATGTGTTCAATGGATCATAACATCAGCTTTCAGCACCTCCAAGACATGTTAAGTCAGACACTCTGT
    TCTGGGCTGAGACCCAACAGTCTCCGTGAACCCAGGTTATACACACACCCAAGCTTAAGAAATGCTGCAT
    TTTGTTGTGAGGGAGAGAATTATTTTAGAATTTGGAGTAATCTCTGCCTAGGTTTAAAAAAACCTAATCC
    ACTACAGGTCCTTGTCAGAACTTGAAAGTTTACCCTGTGTCACCTTTCCCACACCTAAGCTGGTCCTCTA
    GCCTGGGGCATCCCAGTGCCTACTGCACAGCAGGGTGATTCGGATGCCCAGTGACACTGTTCTCGTCCAT
    ACTGGCAGCATGGCAGTACTTTCCCATTCTGTTCAAAGCTCTTTGGGTATTATGTTAATATTACAATCAT
    AACTAAGGAAATATCTGATGACTGTGGACCTGGGAAGCCACGGTCATCTCTAAGTTTATGTTTCTGAACC
    TTAAAAAAAAATAAATAAGATGGGTGTGGTGGCTCTCGCCTTTAATCCCAGCACTCAGGAAGCAGAGGCA
    GGTGGATCCCTGTGAGTTTGGGGTCAGCCTTATCTACATAGAGTTCCAGGACAACGAAGGCTGAAAGTCT
    GTCTTAAGAAGAGGGAGGGAGGAGATGATTGTGGATATCAGGAAGACAGTTCAGCAGGTCACATTACTTC
    TGCACAAGCCTGGTGACCTGGGTTTGAACCCTGAACCCAGAGTGAAAGGAGACAGTCAACTCCCAAAAGT
    TGTCCTCTGACCTCCACACATGCACACATACAATAGTCATACATCAATATATTTTTAAAAAACAAAATGG
    TATCAGGTAGCTGGGCCCTATGATTATTTAAGCTCCAGGTAGCCTCTAGTTCTTTATTTGGAGATGTTTT
    TACCCAAATCTAGAAGAGACCTCCTATAATCCTGGTCCAACAGTGTGAAAAGAAAGGGGAAAGTGAGTAT
    TTGGAAGGCAGATAAGCTGTTGATCGGGTTAATTGGATAACAGTTGACATTCTCTCTCCCATTTCCCCGC
    CTTTTCCTTCTCCTCTGCTCCCCTTCTTCCTCCCTCCTGCCCCTCCTCCACACAGATGTGTCCTCAGAAG
    CTAACCATCTCCTGGTTTGCCATCGTTTTGCTGGTGTCTCCACTCATGGCCATGTGGGAGCTGGAGAAAG
    ACGGTAAGCAGCCTTTTCCCTGTTTTCTTTGGAGCGCCAGCACCTAAACTTGATCTCCAGGCAGCCTGCT
    GGGAAGGGATAGTGAAGTCTGAAGTAGGCAACCGTGCATGGGATTGTCTCAGGAAAAATCCTCAGGGAAG
    ATGGAGGGTTAGCGTCTCTTCCTCCACCTATCTCTCTCCCTTGTTTGGTTTTCATGTTGGATGACAGGAA
    AGGAGTTTCAGGTGAACTATGGTGGTGTGTGGGATATGATCTTGGCCTTAGATACCAGAAAATGACTCTT
    CTAAACAATTGAAATTCCCTAATAGAGAATTTGTAACGTAGCAAGATTGTCTTCTACCCTCTCTTGTCTT
    GCATATTACAACGTGCTCTCAGTGGGCACCATGATGGTTGTCCTCTGGCTATTTTGGGATTGGCATTCAC
    ACTGACTTGGTTTCAGGTCATGGCTCTGCCAGGTATTGATACTGAACCTTTGAGGCTGGGTAGTCTTGTG
    ACCTCAGTTTTCTTTCCTTTTTTTTTCCCCCAGACAGGGTTTCTCTATATAGCCTTGACTTGTCCTGAAA
    CTCACTCTGTAGACCAGGCTGGCCTTAAACTCAGAAATCTGCCTGCCTCTGCCTCCCGAGTGCTGAGATT
    AAAGGAATGCACCACCACTGCCTGGCTGACCTCAGTTTTCTTAATTGGGTGTTCAGTATAGCACAGCCCA
    CATGGACTATTGAATATGTTAATAAATACAGAAAATAACATATGGTAGAGTGAGCAAGCACTATTATTTA
    AAACAAAAAGTCTCTGAACTTTTCATTATCCCAATGGCAATAAGATTATTAGTAGACAAATAATGAAGGC
    ATAGAAGGGGGTGCTCTCTGTCCTCTGTCCTTGTGCCATGTCACAGTTAGCAAATGTCCATCAACTCAGT
    CCTCAGATGGTCGTCACAGGATCTCAAATGTTTCATGGAATACAAGGTAATCAAGTCAGGAATTTCTGCT
    TTTATAGCTACACTTTAGTAATAATTAATATTTATTTAACACTCAATTTCGCAACAACCATGTAAAGTAG
    GAGCTATCTGTATTCCATTTTACAGATAGTAAAAACTGAGGTTCAAGAGGCTAAGTAAGTTTCCCACAAG
    GTCCAGAATAGAACTTGATCCCAGTTTAGTCTCACTTCAGAGCCTGTGTGCTTAAATGCTAGATTTTACA
    AGTAAGGCGTGAAGCAACTATTTCCTGTGGCCATAAAGAGGAAATGGGTGTAAATTATAAATGAAAAGAT
    TTCCCAGCTGCTGATTAGAAATAATCTTCTGAGAATCCAGGAAAAAAAAAAAAGGTGAGCCTTTTTTTCA
    TTCTGAAGAATTACAAGAGTTTGAACGATATTCGCAGGTAAAGGTGCTTGCCACCAAGTCCGATGACCTA
    GACTTCATCACTGCGATCAGCATGATGGAAGGAGAGGATCGAGTTCTCTAAATTGCCCTCTGCTCTCAAG
    ACATGAGCTGTGACACCTGTGCACACACACACACACACACACACACATGAAAGAATTAAATGCAACTTTA
    AAAAGAGTAATCTCCAGCCTGGCCACCCACGCCAACAGAATTTTGGATGCTTTCCTAAGTAAAGCCTTTG
    CCACAGCCAGATGGACAACTTAGGCCCTGGCAGAGTTAACCCCTTAGTTACTTCTGACCTGATAAACCAC
    AGAAATTTCAGAAAAGCAACAGGAGAAACACAGAGAGAGCTTGTGTTGGAGTCACAATGGTGGCCCGAAG
    CCCAGCAGATATCTAAGCTCTGATTCCAACGTGTCCAAGAAGTTCTTTCCCTGCCCTGGGGCTAAGGTCT
    TCTAGGGTTTCTGAGTTCAGGTTCTAAAAGGAAAATAATAGACAGCCTGTTTCATGGCCTAGGGGACAAA
    GGGAAACTGGGAGCCAAAGGGTCCTGTGCACTGGAATCAGCTCAGGCTAACGATTTCTGAAGAGCTTGGC
    CTCAAGTGATTTGTACCTCTTATCTTTGCTCCCCATTCAGCAGTGACAAATCCCACTGATGTAAAAGTAA
    GAGCTTAGCATTTCACAAGGCTTTAAGCTCCAATGCCAATCACAAAGCCACCATTCACAGGGCACTTAAG
    GAAACTGTCTTAAGCTTGCATACAAATTAGTTTAGATGTTACCTTCTAAAGAAAGCACTAATATTTTGCT
    TTCTTTTTTCTTTTTTTAAAAGAATGACATCAGACTAGGGCATGGCAGTTCATGCCTGCAATCCCTGAAC
    TTGATAGATCAAGACAAGAAGAAAGCCCAATTTTGTGGCCAGCCGGGGCTATAAAGTAAAATTCTAAGTA
    GCTGGCTGTCTCTTCCCACCCCCGAGGCCATGGCTCTCTGAAAGCCCTTTGCTGGCTCTGCTCCATAGCC
    GTGCTATTCCAAAACCCAGGCTCTTCACCACTATGCCTTCCATATTCAATCAGGCATCAGATGACTGGGG
    CCATCCTGATCTGACTCAGCAACCAAGAACAAACCTCACTGATGGGTTTTCAGGTGATGCTGATGATGGA
    GGTTGAAAGACGACCCTGCTTTACGGGTCACTGCATTAAGCTAGTCTTTCGATTAATAATTGATATGTAG
    GTAACCTTTGAAGCTTTGTCACATGAGGCTTTGGTGTGACCCACTCCAGAATACCTTATGGAGATATTCT
    GGAGGCTCCTTCAGAAACTAGCATCAGATATCCATTGCACAACACAGGAGACAAAAGATCCGAAAGACAC
    CATTTGTTACCCTTCACACTTGATGGGACCTCACTTAGCCCTTGGCCATCAGCAAAAACAGTTCCTTCAA
    AAACTGAGACAATCATCTACTGCTTTGTAAGAGTGTAAAATAGCTTTTAAAAACAAGTAGTTAATGGCAA
    TCTCCGTCCACAAATTTCATGCAGAAGAGACTTAAAAAAAAAAAAACCTAAATCTTTCTGGATAATTTTA
    AGAGTTTTTCAGTGCATTTGCCTGGCTCTTTGGCCTGTGCATGACAGTGATGATGATGATGGTGGTGGTG
    GTGGTGATGATGATGATTTTAAAAAGCTAGCACAGGGTTATCCTGCCGTAACCTTAGCTGTGCTAAGGTG
    CATGCTTTTTTACCATCTATCCTCACAGCTAATCCTATTAGCATAGACAATATTGTTCTTACCATTTTGT
    GAGGACTCTGGGGCTACTGGTGGTTCAGGACCTTGCTCAAAATCTCCTTTGCAATTAGAGAAGATAGGAT
    TTCTGCATCTTTCTTTCTCCACACCTATGCTTTAAGCCACTATGTTATTCTGCTCAATACTCTGCCCTGT
    GTATTCCCTGTGAATTACAACTGGAAGCTTACTCCCCCTCATCTCCATTCCCAACACACACACACACACA
    CACACACACACACACACACACACACACACTAGTGGCTTCTGACAAGGAATCTTGTGCTGTTTGCATCTTT
    TCTTGTTTCAAGGTCTTCCCTTCCTAATCAGAGACCGATTATTACTGGCCACCTGCCAGTTAAATGACTC
    GGTGGCAGCTATAGAACTGTTTCTCTGTTCTGTCCCCATGCCCTGAGATCTCTCAGCAAGCACTTGCCAA
    ACTCCTGTGAGCTATGACGTTCCAAGGGCCATGGGTGTGTCAAGACACTGAGTGAAACCCAGTTCCCGGG
    GTTTGTGAGGCTCCTGGTCCAGGTCCTCACTGCTGGTGCTCTCCAGTTTATGTTGTAGAGGTGGACTGGA
    CTCCCGATGCCCCTGGAGAAACAGTGAACCTCACCTGTGACACGCCTGAAGAAGATGACATCACCTGGAC
    CTCAGACCAGAGACATGGAGTCATAGGCTCTGGAAAGACCCTGACCATCACTGTCAAAGAGTTTCTAGAT
    GCTGGCCAGTACACCTGCCACAAAGGAGGCGAGACTCTGAGCCACTCACATCTGCTGCTCCACAAGAAGG
    AAAATGGAATTTGGTCCACTGAAATTTTAAAAAGTAATTCCATCCCCCTTGACAGTATCTTTTCTTCTAC
    CCCTCCTTAAAAAGTAATAATTGCAAGTGTGTGTGTGTGTGTGTGTGTGTGCGCGTGCGCGTGCGCGTGC
    ACTGAGGCTCAGAAATGTTTCTCATTTCCTTTGAAACAGGAACTCTCATTGGCCCATAGCTTACCAATAA
    GCTTTCTTGGTTAGCCAATAACATCCAGAGACCTTGTCTCAAAAAAAAAAAAAAAAAAATCTCACCCTTG
    TACCATGTAATGGCCAGTGATCCATCCACAGTAAAAAAGAAATAAATAAATAAAATAAAAATAAAAAATA
    ACAAAAACAAATTTCTTTATTAAAAGAAAACTCCTAAATATTTAATTTTTCTTTTCTTTTCAAATGCAGA
    TTTCAAAAACAAGACTTTCCTGAAGTGTGAAGCACCAAATTACTCCGGACGGTTCACGTGCTCATGGCTG
    GTGCAAAGAAACATGGACTTGAAGTTCAACATCAAGAGCAGTAGCAGGTAAGGGAGCTGCCATGATTTCT
    CAAGGTTTTGTGTGATCTACTTCATAACAAAAAGAAGTACAAACTGCCCATGGATTATCAGTGACTGGTA
    ATGGAACAGCAAAGTCAATTCTGTATTTTTTTTTTCAACTTCCCTCTGCTCGCCTAAGGAAATAGCATGG
    AGCTTTAAGCCAGCCCTCTGAGACTAGTTGGCTGGGTAGTTTGAATTCACTCATCCGAATTGCCTGGCCG
    TGCTTCATTAAGGCTCACAGCACCATGGGGAGGAATTAAAAAGAAGCCACAGCTACAAATAACTGGCCAG
    AGCTCGACCTTGTGTCAAAAGCAGAATTGAGTGCTCCAGGGCCTTGCTGTTGGAAGCCCTGGAACAGATT
    CCAGAACCCTCTGAGGCCTTTCGAGATTTTCCTAGAGCCTTTTCAACTTCTTCCCCCCCCCACCCCCACC
    CCCCAACCGTTATTCTGAAGTTCAGTTTCCCCAATGCACTTGGGCTTTTCCTAAAAACATCATCTCCCAG
    GGCTGCAGAGCGGGTGTGTCAGACTGCTCTACAGCTGACTTTAGTCAGCAACTAAATCCCTGAGCCACAT
    GGAGGGCAGGGAGTGAGCGCCCTGGCTCTCCCACTATTTAAATGAATAAACGGGGGACAGGGGGTGTGGG
    GGGGACGACTCCCTTTTCCATCTTTATCATGAATTTGCTATGACTTAACTTAGCTAAGGAAAATAATGGT
    TCAGGGTGACATCAGAGTCCTTGTTGCTTCTATGAGAATTTTCTGATCTGGTAAAGGTGGCCCCAAAGGA
    GACCAGTGTACAATGCAGATGCTTACATATAGAAACATGCCAGACCTCTATGTGCTTTAAAAAATTAGAC
    AAGCACGATACATTTTCCTGCTAGAGAAATCTTGCACGTGTTCCTGAAGAAACTAGTACATGGATAAGGG
    TCAGTGGAACATTTTGCATAATTAGCAAAAGCATGCTCAAATGGAACATCAATGTGCATCTATTGAAATA
    AATAACTCAACTTTCGTGTTCTGTTTAGAGAACTCCCCCACAATACTTTCAGAGAGGAAAAAGACGTTAC
    AAACTCATGCATATATATTTTTCATTTGTCTTTAAAGTTGTGCTACTCTTTGGATAAACTACATATGTAC
    ATATATGTCTGTGTGCATGTCTATCTTAAATATTCATTTGGAAAAACTCTAGAAGTAACAAACTGTGAAC
    AATGTCTATATGTGGGACTCAGATTTGAGTAAAAAGGAGAATTGTGGCGACACGCATGTTTTCTTTATAA
    TCATCTTTGTTGTTATAATTCTTTATAATTCAGCAGTTTCTCTTGCATTACGATATTTTCAGGTTCATTT
    AGGCAGAGATGCAACTTTCAGTCTCTTACAGCTAAGCTTCCACTCTGCAAAGGGCAAGGGGGTTTTTGTT
    TGGTTTTGGTTTTGGTTGTTTTATTCCACTGGAGAAATCCAACCAGCTGGACAGACTGGCCTCGGACTGT
    AGACACCAGGCACTCTATCTCTGATCTATAATACAGAACTCATTCTGTCTTTGCCATCAGTTCCCCTGAC
    TCTCGGGCAGTGACATGTGGAATGGCGTCTCTGTCTGCAGAGAAGGTCACACTGGACCAAAGGGACTATG
    AGAAGTATTCAGTGTCCTGCCAGGAGGATGTCACCTGCCCAACTGCCGAGGAGACCCTGCCCATTGAACT
    GGCGTTGGAAGCACGGCAGCAGAATAAATATGAGAACTACAGCACCAGCTTCTTCATCAGGGACATCAGT
    GAGTTTTGCCTGGGAGCAGGTGCTAGGGAAGCAAAGACGATGTCTGCCACGTGTCCATGATACCCTGGGG
    CATAGGATTGGCCTGCATTCTTTTCCATTCTCCTAAAAAGCATGATACAGACACTACCCTCTTCATCTTA
    CAGATGAGGAAATTAAAGGGTTAAGCTAACCTTTTTCCAGATCTCAAATCTCACAGACAGCAGACCAGAC
    CCAGACCTGCCCCAATGGTGGCACCTAATGGATTATGGGTTAACTACATGCTTAGTGTAAAGCAGAAACC
    AAGCACAGGGAGGGTTCCCTGATTGCTGAGCCCAGATACGAGACTCTATTAAGCAGAATGACTCAACCAG
    TAGGGACTTCCGGTCTGTGCAGTGCAGAAAGGGTTCTAAAATGTAGGATATGGCCAATAATGGTGACTTC
    CTCGAGTGGAAAGATCACTCTCAGCAGATCCCTCACAGGTGCTGCCCTGATGCTCAGAGAAGTGAAAGAG
    CTATTCCAGAGACTAGAAGGTGGAGGGCTCTGTCAGCCAAGCTCAGGAGCCTAAGTCCTAGCTCCAAAGC
    CTGGCCAGGATAATAAAGGATGCTTGTCTTCTTCTGCAGTCAAACCAGACCCGCCCAAGAACTTGCAGAT
    GAAGCCTTTGAAGAACTCACAGGTGGAGGTCAGCTGGGAGTACCCTGACTCCTGGAGCACTCCCCATTCC
    TACTTCTCCCTCAAGTTCTTTGTTCGAATCCAGCGCAAGAAAGAAAAGATGAAGGAGACAGAGGAGGGGT
    GTAACCAGGTAGGATTTATGCAGCCCATGTTGGGGCCATTGGAAAGATCCTGACAACCAGGTGCTTGCCT
    CAGGACCAGAACTGTGTTAAGGGCTCTCCATGTGAATCACACACAACCTATACAAAGTCCCAAAGGCAGC
    CAGTATTGCTGTCACCTCTCCACAGAGGCCAGGAAAGCTCAGAGAGGCTATGTTTTGCCCACGTTCTTCC
    AGCTAGAGCAGTGGCAGAACCAGTACCCAGCAATGGTTTGATGGAGAAAGAGATGGTTAGCTCAACCACT
    TCAATTCCTCCAGTATAGTCGATTGTCAATCCTCAACCAAAGTTGTTCATGATATAGAAACCTGGGATCC
    AGGAGCCTATAATGATTGTTTAAACTGAGTAAATATAGTAAATGCTAAAACATTGATGCTGGGACCCACC
    AAATAAGGATAATCTCTACTTCCTTAATATGAGCAATCATTTCACCTTACAAGATGTCCACCTTTACTGG
    GCTAATATGGTGACTTTCTACCTTTCGGGTCCTTAGCACAGTGATCTCTTGCTAGGTTTGAGGAACACTT
    TTATGAGCATTTAAGTCACAGAGCAAAGGATTCTTCCAACCTAGCATTGAGAATGTGATAGCAATTCAGC
    TCAGAGATTCAACCACTTCCTAAAAGCAGGAGCAAAAAATGGGATTATTTTGAAACAGATTTTAACTGCT
    ACCTAAGTTTCATCCCTGTGTGTATGTATTTAATATACCAATCTTAAATAGGTAATGTCTAGTGCTTTCT
    ACTTGCTAAAAGCTTTCTACGCATTTTTACATAAATGTTGTATTTAGCTGCTGCAATTCTTAGAGGCAGG
    CATCCTGGGACCTACTCATGAATAAGGAGAGACACAGAGAACCAGAGATATTTTCCTGAAGCACCACAGC
    TGTTTAGTAGCATTGCCAGGATTTGAAGTCACTTTTGTAAGCTGACAGGCATGGTAATTCTTGATGTCAA
    GACAGCTAAAGAAAGCTGGTCTCAGTCCCAACATCAGCTTCATATGGGAGTGGTTACCCATGGGACCTTG
    GGGTAATGCTGCTACTTCAAGAGGCAGAAGGGGGAGCAAGCCATTTCTGTTGGAGTTTACTAAATAGAAA
    AGTGAGAGTGGGGTGGTGGATGAATCATGCTGGAATATGAAGACCTGAATTTTAACCCCCAGAACCTCTG
    TAAAACTGAGTAGTAATGCCTGAACCTGGGCTCTGGAAAGAGGTGGGACACATACACACACACACACACA
    CACACACACACACACACACACACACACACACACCTCTGATTATAGCTTCTACCAGCAGGTGGTGCAAGGG
    GAGCGCCCATGCCAGCCTGCCTTATATTGCCTAAAGCCCTTACTTAAATTTTCCTCTCCTTTGCAGAAAG
    GTGCGTTCCTCGTAGAGAAGACATCTACCGAAGTCCAATGCAAAGGCGGGAATGTCTGCGTGCAAGCTCA
    GGATCGCTATTACAATTCCTCATGCAGCAAGTGGGCATGTGTTCCCTGCAGGTGTGTGAGCAGGACCGTG
    AAGCCCAACCACACCTGCACATTTAGTGGTCCTGGTGGAGGACAACAGGAGGCTTGTGAGGGAGGTGGGG
    GTGGAATTCCTGGCATAGGGATTCCAGTCAGAAACACATTTGTTCATCAGCAGATGTTAACTTGATGTGT
    ATGTTAGGTCAGGCACTATGCTAGGCCAAGGATATTACAATAAAGAAAACAAGCATGGGTCCTACCCTTG
    TACAGGGAAAGGCAAGAAGGACCAGAGGAGGAGGAAATCAGGAGTCAGAGTATGGAGAACTTGGAAGTAG
    ACAGAAGTTGGAACCATATCTTAGGGGTTCTGGGGAACCACTGAAGTCTTTTTAATAAGGAAATATCCCA
    ATCAAAGCCCTTTTAAGAAAAATCTCTCAAGAGAGTCCCTGGCAAGCATGAGACCAGCTGAAGTCCAAGC
    CCTGTAAGGAAGGGTTGGGCTGTCTCGCACAGAAATCCTCAAGACCCAGTGTCTGGGGATAATAATGTGT
    GGTGTGAAATAGGAGGGTATTGAGGCTATACCTCTGACCAATATGGACTTACCTACCCACATTCCTTTAC
    AGGGTCCGATCCTAGGATGCAACGTTGGAAAGGAAAGAAAAGTGGAAGACATTAAGGAAGAAAAATTTAA
    ACTCAGGATGGAAGAGTCCCCCAAAAGCTGTCTTCTGCTTGGTTGGCTTTTTCCAGTTTTCCTAAGTTCA
    TCATGACACCTTTGCTGATTTCTACATGTAAATGTTAAATGCCCGCAGAGCCAGGGAGCTAATGTATGCA
    TAGATATTCTAGCATTCCACTTGGCCTTATGCTGTTGAAATATTTAAGTAATTTATGTATTTATTAATTT
    ATTTCTGCATTTCACATTTGTATACCAAGATGTATTGAATATTTCATGTGCTCGTGGCCTGATCCACTGG
    GACCAGGCCCTATTATGCAAATTGTGAGCTTGTTATCTTCTTCAACAGCTCTTCAATCAGGGCTGCGTAG
    GTACATTAGCTTTTGTGACAACCAATAAGAACATAATATTCTGACACAAGCAGTGTTACATATTTGTGAC
    CAGTAAAGACATAGGTGGTATTTGGAGACATGAAGAAGCTGTAAAGTTGACTCTGAAGAGTTTAGCACTA
    GTTTCAACACCAAGAAAGACTTTTTAGAAGTGATATTGATAAGAAACCAGGGCCTTCTTTAGAAGGGTAC
    CTAAATTTAAAAGAATTTTGAAAGGCTGGGTATCGGTGGTATATGCTTTTAATTCCAGCACTCAGGAGAC
    CAAGGCAGGCAGATCTCTGTGAGTTTGAGGACAGCCTGGTGTACAGAGGGAGTTCCAGCACAGCCAGTGC
    CACACAGAAATTCTGTCTCAAAAAC
  • Protein Sequence : Show Sequence
    >gi|6680397|ref|NP_032378.1| interleukin-12 subunit beta precursor [Mus musculus]
    MCPQKLTISWFAIVLLVSPLMAMWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIG
    SGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCS
    WLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELAL
    EARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEK
    MKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG) , MVA/IL-15/5Mtb vaccine
25. Il15
  • Gene Name : Il15
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16168
  • NCBI Protein GI : 363000984
  • Genbank Accession : AC156993
  • Protein Accession : NP_001241676
  • Taxonomy ID : 10090
  • Chromosome No : 8
  • Gene Starting Position : 82331623
  • Gene Ending Position : 82402585
  • Gene Strand (Orientation) : -
  • Protein Name : transcript variant 2
  • Protein pI : 4.96
  • Protein Weight : 17600.72
  • Protein Length : 162
  • Protein Note : Also known as IL-15; AI503618
  • DNA Sequence : Show Sequence
    >gi|372099102:82331623-82402585 Mus musculus strain C57BL/6J chromosome 8, GRCm38 C57BL/6J
    GTTAAATTTCAATTGATAAAATATCCATACAACTTTATTCCACATCTTCTGTTATATTACAAATATGTAC
    AAATGAAGCAATAAATTAAAAATAAGCAAGCAAAAATAAGTCTGAGACGAGCTCTTTTCCTCTCTTCATT
    TCCAAGATTTCATGTGATCCAAGTGGCTCATTATCTCCTTGTTAGGAAGCTTAGTTTGCCCAGCAGATTT
    CAAATGCATGGAGCTGTGCTGCCTCTGAGCAGCAGGTGGAGGTACCTTAATAACAGAAACACGGAAGAGG
    CTCGCATGCAGTCAGGACGTGTTGATGAACATTTGGACAATGCGTATAAAGCTTTGCAAAAACTCTGTGA
    AGGTTTTCTCCTCCAGCTCCTCACATTCCTTGCAGCCAGATTCTGCTACATTCTGCCAAATAAGGAGGGA
    GAATTAGATCTGCAGTCAGAACACACTTCTTCGTTGGAGGATATCATACTATTGCAACACATGGTGGAGT
    CTTCAGACATGAAAACATTGGCAAATGAAATCAAGTTTTGAAAAATAGTGAGAAAGAGTCAAGAGATTTA
    TAGGGGTTGGGTATTCGTACGTTGGATTAAAATCTTTGCAAATGTACAGGACATTCATGTTACATTGTGT
    CCTTCCTGCTTCAAGCCCATCACTGTGTTGTTGTTGCTGTTGTTGTTGTTGTGGTGGTGGTTTTGATTAT
    TTTTCCTTGTTGTGGTATTTCTTTTTCTTATCTTTCGTATCTCTCTCAAAGTCTGTACTTCATGAGCACT
    TGTCTTTCTGTTCTATACATATAGTGTGTATGTGTGTGTGATATATATCACATATATAATAGTTAGATAT
    GCATATATCACAGACTTTTGGCCTTTTTACACCACTTTTAGCCTTTTACACTACTGTTGCCTAACTACTA
    TTCTTGGGAAGCATAGCCATCTATCTTTTGTTTCCCAGCAATAAACACAAGGCTTGGCACAGAGTAGCTA
    ACTGAATATTATTGGTTGATCTGAACCACTTAGGCAAATGCCATAAACATAGCTTTGGGGACAGCTAGAA
    TACTATAAGTGTAGCCACCATGAATAAACATTTTAGAGATCATCTGAACCACGGAGGCTGTGCCAGCAGA
    AATCAAAATTATATTTCATGTTCATTAGGCACTTGGAATTCAGATTCCCCAATATGTATAAGGCACACAA
    TGAATGCCAGACACTGCTGTAATGCTAATAGGATTCTCTAGTCGGTCCAGAAACAATTTGAAAGAGGAAA
    GCAAACATGCTTAGTGGTTCATCGCCACTGACATGTAAACTGAAATTCTACACAAGTCTCCTAACCATGC
    AGTCCTGTTCATTCTTCCAGTATTTATTTAGGCCAAACTGTCACTTTGGGGGCTCCAACATGCAGCCTAA
    AGCTCAGCATTGATACTGTGATGAGTTATCTGTTACCATTAATAGGCAATGATTCACTTGGTAAGAATCC
    CAAGTAACGGATACCTGTAAGTTTATGTACTGGGAATTGTAATAACACAGTCAAAAGAACTCTAAAATGT
    ATGTACTCTGATGCTGACTTTCTAATGGTGACAGATGATTTCAGCTACTGACTCTAGGGCATAATTATCA
    GTGTGGGGCATATATATATATATATATATATATATATATATATAGGTGGGAACAGTGAGCAAGGCTGTAG
    GGAAAGCATGGCAAATATGAATAAGTGATATCACTGAGGATGTAGCATCTGAGCAGACACTGAAGCAGCC
    CAGCATTTGGGAAGAGAAGCCAAGACATCTCAAGATCACTGTACCTCCAGTTGTGTCTGCCTAAAGACGC
    TCCCGGAGTGTCCCTGACATCCATTCCCTGACTTCTGTGTAGCATACCACCTTATTGACTAACTGCTCTT
    CACAAACACACACACAGACACACACAGACACACATAGACACACACACACACACACACACACACACACATA
    TTACCTCCTTCCTCAGAACATGTGCTTCTTAAAGGGATATGTTGAAGTGCATACTAAACGAAACAATTTT
    ACTATTCCCCCTCTGATGATAATAATCCTTTGTTTGAATCTTGTTTTCTCACTATGGTTGTCCCTCTCAG
    GCCACAATTCCTTGTACCTCAGGACATTTGTGTCACTTACCCTTTAGCCAACCACAGGAGTCACTAGTAT
    TTGTTTCTTGTAACCCACTCCTGCTCATGTTGTCTGGGATGTTCTAAATAATACTTGAATTGTTAACACT
    TTTCATCATTCTAATTTTTCCATTACTTAAACGTTTAGAAGGTGAGAGGGCAGAAGCTGGAGTAGAATCA
    CAGCTCTAATGCCTTTGGCTGCACAAACTGGGGCATGTGGTTAGCTTTCTGCTTCCCATGTGCATCAACA
    TTCGAACAAGAACAGAAATAGCCAGCCATGAAAGAAGGGAAGTGACCAGTCTCTGAGACTGATTTTGAGG
    ATTTAGTACCTTATCAGTCTAAAGTATAAAGATCAATGTGTGCTATTAAGACCTAACCCAATAAATGTGA
    GCTACTGTTTCTGATGCTAAACACTTGATCACATTAATGTCTATCCACTGCACCCACTTGCCAATAAGAC
    ATTTTATTTTAAATCAATATGACTCTGACAGGGAGCACACTGGTTGGCATGATTCAGAAATGTCTGTTTA
    CCAAGGAGACCAACATGTTTAGGATTCTGTGCATGCCAGCACTGGTACCTCTTTTGGACACCACTCCACT
    GGAATCAAATTCTCTTCTGTGTGAACCACAGGAGACTCTCCTTATATATACATGCCTTATTTCAAGCAAG
    CCAAAACTAGTCAGATGCAGCTCTCTCTGGATGCTGGACTACTCACCTTGTTAGAAGACAGAGTGCTGTT
    TGCAAGGTAGAGCACGTTTCTTACTGTTTCATTAAGAGTCATGTTACTGTACTCATGTAAAATAACCTGC
    AATTCCAGGAGAAAGCAGTTCATTGCAGTAACTTTGCAACTGGGCTAAAAAGAAAAGAGCCAATTTAAAA
    AAAATAAACAAAAAACCTTTTCCCATTATTCATTTTCATATTGATAAGACTTTGATTGAAAAAAAAAAGT
    CAAATCCATCTAGTTATTTTACAACGAAACGTAATATCCACACTGCTTAGTTTTAATTGTCAACTTGACA
    CAACTAAGAACATAGTCTTAATGGGGACCACTTCTCAGATAACCTGTAGGCATGTCTGTAGAAGCTTGTC
    TTCCTTGTTAATTCATGGAGGAAGGGCCAAACTAGTGTGGTGGCACCATTCCCTAGGCAGAGGGTCCTGA
    ACAGTATAAGACAGAGGAGAGCCACCTGAGAGCGAGCAAGCAAGCAAGCATTCTCGCAGTTACTGTGTCT
    CTGTTCTTCACTGTTAGCTGGATACTTTAAGTTCCAGTCCTGACTTCCCATAACTATGGACTACAACTTG
    GAACTGTAAGCCAAATAAACACTTTCTTCCCTAAATCACTTTCCGACTGTGTGCCAGCAGGGAGTTTTAT
    TTACACAGGAAGTGCCTTTCTTTTTCCATTTGATTTTGAAATAAAAACCAAAACCACTAATAGAGAAGGA
    TAGTACAGAAGGAAACACAGCATAAAAGGCTACACCATCTCTCACTAAACGCCCTCTTCCAACGGTTGAA
    TGAGAAGCCTAGAAATGACAGAACATATGTAAAAAGTGTTTAGGGTCATACTACATGTTCACATCTTACA
    GAGTGGTAACTGTAAGTCACACTGTCCCTATAGTTCTCAGCAGAAAGAATTCTAAGACATTTGAGAGAAA
    ACCCAAAGCAAAGGCAGTGCCCACTTATGCACTGGCCAAAAAGGGACTGTGGACAAGAAAATGTATTTCT
    TGTAGGAGTTACTTCACAAAACCCCAAACTTAAAAAAAAACAAAACCAAAACCAAAAAAAAAAAAAACTT
    AAGGAAACATTAAATGGAAAATTAGCTATGCCTGAGCCTCAGTATTCACATCCACACTTCAATACTGGAA
    GCTTTGCCTCACCTTTGAGTTGCAAGGCTATCATCCTGCATGGGAAAACTTTCTAATTAGGAAAAAAAGC
    AGAGAAAAACAAAAAATATGCTAAACTCAGATGAGGAGTTATCTAGATCTCACACACTTGTTTGGTGGAC
    ATTTGCTTGTCTGAGTTTCAGGCTCTACCTTCTATGCAACAAACCATGAGCAGAGAGCACACACCTTCCA
    CCTTGGAGAAGTTCCAGGTTCCAAACACAAAATACACAGTTTGGTTTTGGTTCTAGGTTCATTGCTTTCT
    CTGTCTCTGTGATAAAATACTCTGACAAAGAGTAACTTCAAGGAATCCGGGAACCAAGATGGCTCCCCCA
    GGTGCTCCGGCAAAGCTCCTTCTTAGAATTGGGAACAAAACACCCATGGAAGGAGTTACAGAGACAAAGT
    TTGGAGCTGAGACAAAAGGATGGACCATCTAGAGACTGCCACACCCGGGGATCCATCCCATAATCAGCCT
    CCAAACGCTGACACCATTGCATATGCCAGCAAGATTTTGCTGAAGGGACCCTGATATAGCTGTCTCTTGT
    AAGGCTATGCCGGTGCCTGGCAAACACAGAAGTGGATGCTCACAGTCAGCTATTGGATGGAACACAGGGC
    CCCCAATGGAGGAGCTAGAGAAAGTACCTAAGGAGCTAAAGAGGTCTGCAACCCTATAGGTGGAACAACA
    ATATGAACTAACCAGTACCCCCAGAGCTCGTGTCTCTAGCTGCATGTATATCAGAAGATGACCTAGTTGG
    CCATCATTGGAAAGAGAGGCCTATTGGTCTTGCAAACTTCATATGCCTCAGTACAGGGAATGCCGGGGCC
    AAGAAGTGGGAGTGGGTGCGTAGGGGGTTGATGGGGAGGGTATGGGGGACTTTTGGCATAGCATTGGAAA
    TGTAAATGAAGAAAATACCTAATATAAATAAATAATAAATAAATAAATAAATAAATAAATAAATAAATAA
    ATAAGCAACTTCAAGGAGAAAGAAGGTCTGTCTGTTCACAATTTCAAGTCATAGCTCATCATCAAAGGGA
    AGTCAAGGCAACAGACCTTAAGGTAGCTAAGCAGAAGCATAGTTAAGAGCAGAGAGAATGTGTTCAGCTG
    TGCTCAGGCCTTCTCCAGCCTTCTCTACTCTTTAGTCCAGGGTTTACCCCATGAACTGATGCAGCCCACA
    TCAGAGTGGATCTTCTCACCAGGATTAAAGCATTCGGGAGACATGCCCCCAGGGGCAAACCTCACTGATG
    TTCTTTCTAGGTAACTTTACATTGTGGGAGGTTAACAATTATTTAAATTAACATTTTCTGAGCAATGGAA
    TTGGAGATCTATTTCTTCATTTTAGCGATAGCCTTAGAGTAAAAGGAGAAACCAAGTGACCAAATGCCAT
    CAGCAACATCAGTATACTGTAATGAGGCTGCCTCATCCTTTAGAAATTCTAGTACATCATTTGGATTATT
    ATTTTATCTCACATAGAGACTAGTGGAAATATTTCTTACAAGTGTAGAGATCCATACAAAATACAAAGCA
    AGGATGTCTTTAAGCAAGTTTCAGTACATGTGCTGAGGATGTTCACACACTACAGCACTAAAGGAATGGG
    GTAAAGAAGACATTTAGATGGGATCATTTAGTCCCATTCTAGCATCTAATTATATGATGATCTAACTGCA
    TCACAGAAATATGTAACAGATTTTGTTTGACATTGATGAAAAGTTGTTTTTTTTTATTCCACTGTGATTT
    GACACCATTATAATAGTAGCATTTATGTAGCATTTTTCCATGAGTCCCAAAAATTCCACACTCCCTCAGA
    AATATCATGGATCAAACACTAAGAGAACCCACAGTAAGTTAGATTTTAAAACTATAACTGTTTCAATGAA
    GTTATGGAAATACGGCTGATGAACTGGAAAAATAGCAATTTTGAAAAACCTAACTATACTCACATGAAAG
    TCACTGTCAGTGTATAAAGTGGTGTCAATATGAATAGACTGAAAAAGAGAAAAGGACGTCATATGAAAAA
    TCTAGGTATGATACTAGCAACTTCCTGTAAACAATAAATTTTGTCCCTTAACTTAAACGTGAATTTTGCA
    ATCATTTTAAGCTCCTGTTATTATAAGAGAACATATTGAGAGACCGCCTGACCCTCCTCTGCATTTCCCA
    AGCAGGGAAATTCATATTGATGTAACATTTCTCTCTTCTCCAGATTTACCAGTTTTCATCCCCCTGTCCA
    ATCCTAGACCTTTAACATTTCAAGAATGTGAAAGAAATGGAGTTATATAGAATGCAACCTTTTAAGATTA
    GCTTTTTAAAAAATAACATTTTGAGAAGTTCTGTTATAGGGGAAAGACAGCAGAAGGGCCAAGGTTTCTT
    CACCCCTTAATCTGACTCCAGTTCCCTTCATGGTTAATGTGCCTCACACACCATATGTGATTGTTATACC
    CAGTGTGTGGACACCCCTACATAGCTACAGTGTGTGTTTAATGCCCTGAAAGTCTTGGTTCCAGAATAGA
    AATAGTGAGCCACTGGAGGGCAACAGTCACTGGGATTGGACTTTGAAGGACACTGTAGGACTTTTATCTC
    TTCATTTTTTCTTCATTTTTCTTTTCTAGAAAATATGTGAGCAGATTGTTTTGCCATTAGAAGATGGCAG
    TCCCACCAGAGACCCAAAAATAAATACTTTAGTATATAATATAATAATTATATAATATGTATTCTATGGT
    TTTTAAATATATATATATATATATATATATACAATGGCAGGTACCTAGCATTCTAATGGCACACATTGCA
    GTGGAACTATGTTAAAGAACACCATACTCTCCCTACTCACCCCTCCTTTTTCCGTCTGTCTTTTTTTTTC
    TGTAATTTTATCATCTCTCCAAGGAAAAATAGTTGGACCTGCCCAACATGAAACATGTTTAGACTGGGTT
    CTTTCACTGCATGGCCGGCTTTTGGGGTATCTGTTATCTGTTTCCTTTCATGCCTTAGCCACATCTATCT
    CTTGATCTACTGAACGGGTACACCAAAAGGAATAGCTTGGCAAATAAAAGTGGTGAGAGATTTGTTTTAC
    TCCCAGAGGAAAACTGTCTTTCCCTCACTGGCAAAAGATTAAGTATTTTAGTAATATTTTATATTAAGTT
    AAAGAAGTTGTCCTTTGTTTCTATTCTGCTGAGAAATTTTATCTTGAAGTAGCTCTACAGCAGTTTGCTG
    AAGCTGCTATAACAATTTCATATATGCTGGATAGCGTAAGTAAAGACATGTACTTTTCTCGAATTCTGAA
    GACAATCAGTCTCAGATGGAGATGTTAGGAGTGGTCTCCCAAGACTTCTCACTTTGCCTTATCAATTCTT
    ATCCTATCCTATCCTCTCTCTGTCTCCTCAGGGTATCTTCCTCCTGTGGATACCCTTCTTGGAGCATGGT
    TTCCTGCCCTTCTAAGGAAACTGGACATATTAGATTAGAATCATCCTAATGGACCTGTTTTCACTTAAAT
    TTCTCTTTAAAGACCCTCTGTCAAAAGACAGTCACACAATGGGATATGGACATTCATTAAAAAAGGAAAA
    TCCATCCTGTATAAGAGGTACTAAATTTATCATGTCTTTTCTAAATCAATTGATATGATTTTCCTTTTTG
    GCCCCTGATATGTCAGCCTGAGTTGACTGATAGTTCTTGAAATAATCAAAAGACTTGCATAACTAGAATA
    AATTCTAAATTCTATACAGTGATGATATAGGATTCTTTTTATAATACACTGGTAGATCTGATAGGTTGAT
    ATTGTACTGAGGAATTTTATACTAAGTACATGAGAGTTGCTTATATTTGGTTTCATTTTTTAAAATGTAT
    CTTTATCAAGTTTTCACATCAAGGTAAGATTAGTCTCCTAAACTGAATTAGAGGGACTTGCTTCTTCCTG
    GGGGGGGGGGGGAGGCATGATCAATGTTTGAAGTAAGTTTCAGGATGATAGAGCAAGGCTAGTCGCTCCC
    AAGTGTTCTTCTAGGAGGCTCTCCATGGTGACCTGAACAGTGTCAGGAGTTGTAGGAGCTCTTGAACCAC
    CAGTTTCATCATTGTTATGTTTTGGGGAGTTCATGATCTTTGAAGAATGTTTCCATTTCCTGTAAATCAT
    TGGTACAGTTATAAGACACTGAGGGAAACAGTGACAAATGTTATCCCCACAGATCCTGTGTCAATCGCCA
    CCAATTCCATCCATGGTGCCTGGTACCATCTCTATTATTGTTAGATGTTTACAAATTTTATTGATTTTTT
    TTTACTAGTGCCTTTATGTTTCATTGATTTGCCCCCTGACTTCCTGATTTCAGTTTCATTAATGCCTGCT
    CCCAGATCTATTTTTTCCTTCCTTCTACTCATATTGAATTCATTTTGTGATTCTTCTAATTTGGTGAGAC
    AGAACTTCAGGTGCTGGCTTGATGCTTAGTGCTTTTCCTCTTTTCTAATTCACGCATTGCATTTTTAAAG
    TTTCCTCATCTTTAGCAGTTCACATTGTAACAGACAGGGTTTCATTTTATTCATGTCTGTGAGGTTGTTT
    GCGTATCTTTGGGAATTAGTCTTTGACCCATGTGTTACTTAGAAATACGTTGTTTAATTTGCAAATTTGG
    GGGACTTTCCTTTTGTATCTTTTTATCTCCAGATTGATTCCATTGTGTTCTAAACATATTTTCTGCACAA
    TCAAAGTTCTGCTGAGTTCAATGCCCCTTACTTTATGGCTCGGGAGATATTTAGTCTTGGTGACTGTTTT
    ATGTGAATTTTTAAAAGTTTGTTTTGATGTCGTTGGGTAGAATGCTTCATGCATAAAGTCAAATCCTATT
    AATTGTATTTGTTATGGTTCCTATGTTTTTGGAATATTGCTACTTAATAGTTTAGTTAGTTATTGGGCAG
    GAGATGCTGAGATCCACAACTGTAATCATAAATTTGTCTGTTTCTCCTTTGAGCTGTTAAATTTTTGTGT
    CATGAATTTTGAGATTCTGTTGCTTGGTGCATATATATTAAAAATCAATGTTTCTTCTTCATGAATTTTC
    TTTTGTCATTAATGTTGCTCTTGGCATTGGCAGCTCCATTTTCCATCATGTTGGCTTATCTAATACTAAT
    ATAAGACTAATCCTGCTTTTGATTGTGACTTTCCTCTTCTTGGTATGATTAATGCTTTTTCCCTGTGCCT
    GGATATGCTGGATATTATGCTAGGAGACTCTTGTCCTACTTAAGTATTCTATTTTAATATGCAGCCAATC
    ACCTATTGAGATGTTTTGTGCTCAGCCTGACTTACTGTTGTGTGCTGTGATTCTAATGGCAGCTTAGCTT
    TCAGAACCTCTTCAAACCTTCCACTGTAATTCTCAATCACTGCAGTAAGAGTTGCTTCTTCATACCAGGT
    TATGTGGTTCATGGGGACAAAATATATATTTCAGGGTTGTCATTTATGATGAGATTCCTAATCTTTCTTG
    ATTAGATTGAGCCGAACCTGCTACTAGGCCATCTAGCAACTCTAGGCAGGAGAAGATGATCTACAATGCC
    CAGAAAAAAAATACCTCATTCTAGGGCTAACACCACATTGGGTCCCCTTCCTGTAACCCATTTTCCAGAT
    AAATAAGTTTCTGATTTGTGAGTTGGCAATTGAATTTATTTGTTATTCCTTTAGTAGTTCCTTGACATTT
    TTGTACACCAGTTTTGATACTTACTCTCCTTCTAACTCCTTCCATGACCACATCCCGTCCCTACCCACCA
    AACTTTGTGCCCTCTTCGTTTCTTCTTTCTTAATTAAGTATGATCTGTGCTGACCATATACTCTTATTAG
    ATGTGTGGCCATCCACGGTTGCTAGGTAACCTAACCAGGGACAACACACTTAAAGAAAACTTACTTCCCT
    TCTCTCAGCAACTACTAATTGCCAGCACTTCTTCAGTTGGGAGTGAATTTTATGTTATTTTCCACATTTC
    ATGTTAGGATTTTATTTGACTTGAGATTGTGTGGGTCTGGGGTATACTGACAAATGTGTATGTGTTCACA
    TGTGCAGCTGCCCTGCTGTGTTGATCAAACACTTTCCTTGGAGTCATCTACTACCCTGGGCTCTAACAAG
    TTTTCCAATACCTCTCCTACACAGATAGCCTAGTCTTGGGAAGAGGGTATGTGATACAGAAGTCCCATTT
    AGGGATGAGTGCTCTGCAGGCTCATATCCTCTGCACCTTGACTGGTTGTGGTCTCTGTGTTAATGACCAT
    CTAGAGCAAAACGAAGCTTCTGTGATGAGTTTTGGAAAATGCACTAATCTACAGGTATAATGATGTCATC
    AGGAGTCACTTTTATGCTATGCATTTAGCGAAGAAATGATAATAGGTTCTACATTAGAGTCCAGGATCTG
    TCTACACATAGTTCTTAGCTCTGATAAAGGTGTAAATCTTAACAGTAAATAGAATTTTAAAACAATGTCT
    TAGAAGGCTTTGCAATGTTCATATATTTCTTCACAAATATTATTATTTCAAATGGTATTTCATAGAAATT
    TGTAGAAAAACATAAGACATGCATACTTCATAGCCATTACATGTTTTCTTTCTGGAAAAACAATGATATC
    AATGACCTAACCCAGGACTTGGCTCTATGTGCCATAATACTAACCTACCATGTGGGATGTAGCTAGTGGC
    AAGGCTCTTGTAGACAACTAGCCACTTTTAAGTTGGATTGGAGAACTGCTCAATACTACTACTACTACTA
    CTACTAATAATAATAATAATAATAATAATAATAATAATAATAATAATAAAATTATGGCTGGTACTATATC
    CCTGAGCAAAACCCCATGGCTGGGCAGCCATACGCCCTAGGCCACATGCCACTGATATTTTCTCAGTGGT
    TTTGCTGTCAAATTACCTTCTCAGTATTTATAGTTGTGTTGGTAGACTTGGTATCCGTCGGTAGACTTGG
    TATCCGTCACCCTTGGTCAGAGGCATTTAGTCCCCCCCCCCAGTAGTAAGCAATCAGTGCTGGGGTATAA
    TTGGTCAAAGTGTTATGAAGAAGAGATTGACTGTTCAGCACTAAATAAGACATTTTTATGAACCCCACTC
    CTAGTCCTGCCAAGACTCTGAGGATACTGATGAAAAGGATGCAGAAAAAAAGTTAAAAACCAGAGGGTGA
    GAAGAAGGACAATGGAATACTGTCCTCTGGATCCAATATGGCTGTCACATGCATGAGCTCACAGTACTCT
    GTTATCTGTACAGATCTTGTCTGCCAAAATGACAGCTTAAAAGGGGATGTGGTCTCTTGGCCCAGAACCC
    CCTTAACAACCTGTTGGCATGTGATAGCTACTGAGAGAAAATGTATTATTCCCTTTTGTTGGTGTCTCAC
    TCTTATTTTTCTGTTTCAGTGGATGGCAAAATGGCAGTGTACATACTGGTGACATTAATTGGACTTAATG
    GGTTATCAAAACAGCATGAAACAAAAGATGCGAAGTTGGGGCAGACACAGTGGCAAAATAGAAGAGAGGT
    CTTAAAAGGAGTAAAATGGGGAGTAGATACAATCATATTTTACTACATATATATGAAATTCTTGAGAATA
    AAAGTTATAATACAACAATATATATCAAATATTAAAACAAACAAAAAAAGAAAAATGTGTACTCACTTGA
    ATAAGGCTTTCAATTTTCTCCAGGTCATATCTTACATCTATCCAGTTGGCCTCTGTTTTAGGGAGACCTA
    CACTGACACAGCTGTAAGAAGACACAAAACCAAGCTCTTTGGAGGAGGGTACCATTGTCTCACTGAGTAG
    ATAAGCAACGGAACAATCAAGATCAATTATCTTCTCACTAGGAAAATGTCTTATTCTGTCTCTGTGCATC
    TGACAGCCTGGTGGGTGAATGGAAATACCAAAAATACAGTATAATTTTGATAGTCTAAGCTAATTTGAGG
    AAGACTGTCCAACTGTCCAACTTAGTGATCAGAGAAAGGCTAAATGGATAGCTTATAAGTAATCACAGAT
    GTCAGGAGTTATAATGGGACAAGCTAATAATTAGCAGGTGATCTTCCTGAGATGGCCTGCTTCAGATTAT
    TACATAATCTGTTTATCACACTGATATTGAGATAGCACCTGATCTGTGCCATAGCTTAATGAATGTTTCT
    GAAGTGATTATTAGTTGTCTAGAAGTACAGGCTCCATGAAACTTTTAGGGCTAGTAAGTGTAAGATTTTG
    AACACTTAACACTTCCTCTTAACAACTGTGAGAGTAAATGTCCCTTTTTCATGATGCCCACAGTAATCTG
    ATGCTAGGATGATTTCATAGTCACTGAAGATTTCGCTAAATACAATATGGGTGATTACAAGTTTATTCAC
    TTTGGTTAAAACTCACATTTAATATCCCGAATAAAAGACACACTGATCACGTGACTGTGGAGTGATTCTT
    AAAGCCCCAGGAAAAACAGTTATTCTAAAAAAGACCAGGAACAAACCATGTCAGCCAATGACAATCTGTC
    TAATGAGGACAACTTACATACATGATATCAGTATATGGAACAGTTATAGCAAAAAGCTCAACTGCTTTAA
    GGGATAGAATACTCATATAGAATACTGCCAAGGTGCCGAAATCCTCAGTTAAGGATTTGCACGTCCCCCA
    AGCAGCTCCACAGTCACCTGATATCCTACGCTGAAGAGTGAAGACCTATATACAAGGAAACTGAACATAA
    TACAGTTTCAACATCAAGATACTCAGGGACAAATGATTGTGGCCACTAACTTGATAATAGCTATGACCAA
    AGATGAAATTACCCCAAAATGAAGACATGAATGCCAGCCTCAGTTAAAAAGTGACTGTTTAGAAGGAAAC
    ACAAGTAGCACGAGATGGATGTATTCCTCATATATGGTTTCTGGAAAACATAAATGTAGACAAAATGGTA
    AAACATCTCTAGTTAAAAACATGGAAGACTGAATTTTATTGTATTTTAGGTTCAAAATTGTAAAGGTGTC
    ATTTCACAATCAATACTCTTTATGGAAACAGTGAAATTTCAGTGGCTCTTCTGTTTTGTTTTATTCTAAT
    ATCCAGCTCTCTGTATGTACAATATCTCCAGTAGCCATTTCTCCAATTGAAGGTCAATGATAAACTGACA
    TTCTTAATTACTGACATGTATACTCTTGGGACTACTGAGCTTTTTCTGTCATTATCCTCTTCCTCTTGCC
    CTTCTGTCTTTATTAATAGACTACACATTATAAACAGCATAGATTAGTAATGTCACACAATAGCAAGCAC
    ACTAGTTTTACATTCATTTCATACAAGTATATGGAAATTAAAATACATATTATCACACAGAGAAAAACTG
    GATAAAATTTAAACCAATAAAAGTATTTGGGCCAGGGCTAGGATTGATGTCGTCGGACCTAGGATGAGAG
    AGACAGGAACAAGGGATTCTGTAAGCTCAGGACTACCCTGGTGAGTTCCAGGCCACCCAACGCTACATAG
    TTATGAAATCCTGTCTCAAAAAGAAGTATATCCTTTTTCATTCTAACAGCTCCTACATGAAAACTTAATT
    TGGTTATTTAGGACCTTATTTGTCTGAGATAGACACAGTTTTAATTCTCTGACCTGCCTTGTCAGTGCTG
    TCAGAGATTTTATAGTTACAGAGAGCAGCGCCAGTCAACACCTCAAACACCAGGAGTCTGTACTCTGTGT
    AGACTCTGCTGAAAGATGCCTAACACCAGACCTTGCCCTCAGAGACACAATGTGCGAAGTGAATAGACAT
    TTAGAGAACAAAAGAGACTGAGTGAAGTGTAATAACACCTTAACAATGGCACAAAGAATACAGGCATACT
    GTTAGTCTTGTTTTCCAAAGCCCATGCTACTTATTCTTAAAATCAAGATAAATCTTGGAGAAACAGATGT
    CTGTCGCAGCCAGAGATGTTTAGAGAATAATCATGCGACTTATTATAAAGTAGAACTGAGATTAAGGTGA
    CCTTATAAGAAAAGCCATATGCTATTTTTGTCTGCTTTTTTCCTTACCAAAATTTTCATTACTTAATGCC
    CAGGTAAGAGCTTCAATGTTGAAGATGAGCTGGCTATGGCGATGGGCTTCCAGCTGCCATCCATCCAGAA
    CTCTTCCTGCAACAGAACCAGGATGAAGGATGACTATCAAAACACAAGCGAGTAAGCCAGAAGCAGATAG
    AGAAACGCCTGGTGCTTATGCCACATCTGCTTTCCATAGGGTAGGCTTTGTGTTCCTTAACACACGCATC
    AGATTTAGCCCTCCTGAGAGCATCATTTGGAAAATCTGATCTGGCGTCACAGAAATCCCATGACTGCCCG
    GACTAAAACCATTTTAGTTTGTCTGAATAAAAGAACAAAGACATTATTTCACACAGTGTCCCAGATCTGA
    CAGGTTTCAAGGCCATCTCAATTTGAATAAGAGCAATCAAATACAAAGATTCACATAACCACCAGCTGAT
    AGTCGAATTTACAACAACAGCCTCATCTACAGACTTCACATAGATGTCTTAGGTATGTTCTTTAAAATAA
    AGTTTAATGAGAACACCATTTGTGAATTTTCTATATAACAAAATTATATTTTTTTCTAAACCAAAACAGG
    GGAACATGTGAAGTTTAAACACTCCCATCAAACTCCATTTCATTAAAATATTTAGGCTCAAAATATCTCA
    AATATGGATAGTCTTGCTGTCAAAAATGTTACGCATTAGTAAACCTTACCAATGAAAATATCAAGGTCTG
    TTGCTTCACTGATATAATTTATCACAATCAATTATCTCAATATTTTACACATTTCAGATGCTTCCTAATA
    TTAAAATATTTGCTATAAATTATGTGCAATGTATAACTCTTATTTTTTTACATTTACTATGTGTGTGTGT
    CTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGCTCATACACGTGCCA
    TGGTACATGTGTGGAGGTCAAAGAACAAATTGTGAGAGTTGCTGCTCTCCTTTCACCACTTGGGGATCTA
    GGAACAAACTTAGGCCATGAAACTGGGCAGCAAGGACTGTTACCTACTGAGTCATCTTGCTATCTTGTTT
    ACACTCTACAGTGTATATTACATCATTCTTAAGAGAAAATAAACCTAACATTTACCAGGGTATGAAACAG
    TACACTAGACTGTGTGGCTAGTACAATGCTTCCATGAAGTCTTCACCCCACGTTCCCGTTCCCTGTGACA
    CTGGCATTAGACTTCCCCCTCCAGCAGTGGTTCATTTATTAAACAGAGAGGCTGGTTTACACCTAGGGCT
    GACGTTTGCCCCTAAAGCCTAAGTCCCCTGAGCCCACAATGGAAAGTAAACGCAGCAAAGACTTGAGAGT
    CAGCCAGGGTTTCCCACTCAGCCCGCAATGACTTTTGTGAGGTCTGTTCTTAACTGCCTTGCTTTCATTT
    CAGTAAAGCTTGATTAACTTCTCCTCAGGACAACGTGTGTTCTTTGTCTGGAAAAGTGCTCTGACTGTAG
    TCTTTTTGCTAAGGACCATGGCTTTCCAGTTCACTGGTAACAAGAGCCTATTATGTTATCTCTGTGTTAT
    GTCTGTGTATGCTCCAGGCATGGCAACCCACAAGTGACAGATTACCTGGAAAAGGCAGAGAAATGGATTA
    AAATCTGCGTATGACAAGTGAGCACAGACCAGAAGGGAGCAGGGAGCTTGGAAGTATTAAGGCCAGTGGA
    ATGTGAAGCATGCCCTATTCGTTCCCTACATTGCAGGTCTTTATAGCAAAGTGTTATTTTAGCCCTACCT
    TGAGATATGGTCAGATGTTAACCATGAATGAGAACTGAAATAGTGTGAAGTTGGGGAGAAAGCACCTGAG
    CAAGACTATGTCCACCCAAGCGTTCATTTATGGCAGAAAAGTAGGAGATAACAGTTATCATTACTGTACA
    AATTAAAGTAATTTTCTACCAAGGGCCAGGTAATAAGTAGCACATACTTGGAAAGTCTTATGGTTTTTCT
    TGTAATTACATCAATGTCCACGATAGCTTGAAAATGGCGGCAGATGATGAATGGGGCTGCATCTCAGTTC
    AGCTTTGTCATCAAAATAGGTGGCTGGTGCCTCCTACTTACCAACTGAGAAAACAGATCTTCTGGCAAGC
    AAATATTTGCTTTGCTGATGACATCTGGCTTCTATGTGTCCCTTACTCCAGCTTCATTTAAACTTTACTT
    GTCTTGTAACTCATAGTGGCAGTTTTTCAGTCATTGGCAGTCTTAGAGCTGCCCTTGGAAGGCGTTAGTG
    GAACTCTACAGGAATCTCATCCAGTTCCCATCAGAACGAGCTGTTATAAGAATGCCAACCCACATTACTC
    TCTCACCATGTGCTCTCTCCTTCTTGTGTGTACCTTCACCATGGGGAAGCCACATATACCCCAACGGCAC
    TGCGACCAAGTGACACATTAATGAGGTCACTTGGTGTTGTCCAACACTGTGCATTAAATATGCCTCTTTC
    CTTTAGAGGTGTCCAGCTTCAGGGATGTTAAATCTTACAGTTTTTCACAAGATAACAACAAATGATCAGT
    TAAGATGTTTAATATTTTTTATATGTCATCTCTATAAAGATATGCAGGCCTGCACACATAATTTCAATAT
    TAACATGGAATCCTCTTTCTTAGAGATCTGTGCAGTTCACTCTTGGTTATCAACTTGATTAACCTGCAAT
    CAGTTAAGAAAAATGCCTATGGAAGGATCTGGGTGGTTGTTTCCTTTAAGAAGTGGGGAAGGTAGACCAG
    CATATATCCTTGGGGTGGCCTGAGGGAAAGCTGCTTTTTGCCTGCTTCCCTTCTCCCCCTGCTGGTGAAC
    ACATCTACTCTACTGTTGCCATTGCTGCTGTCATCCTTCAATGACATTGGATGCGGCTTCTTCAGTTTTC
    CAAAATGGACTCAAAAGGAACACTCCAGGCCTTGATTATTAGATCAGCACTTCAGAGGCATGTCGGTCTG
    AGCAGTTACCTCTCAGTGTGCAGGGTCCATTGAACTCCCTACTCCTAACTGTGTAAGCCAAGCTGGTAAC
    TCTCCTTTGTAATAGTGAGTCATTCCATCAGTTCTGTTCTGTACAGAAATCAGATGACGCTAGATCCCAA
    ACACTGCAGAAATTTAGGCCCATGGACCGTATTCCCTGTGTGCTACTACCTAGGCATGTTCCCAAGCATC
    TCAGAGGAAAGACCTGTCTCCCAGCCCTCTGCCTCATCTGGTCTTCTACCATTTTCCTCCCTCTTCTCAG
    GTATAATCACTCTCACAGTGGATTAAATTTGTTATGAAGAAAACAATAAACTGCATACAATATACAAATT
    ACGTCTGAATAAGCATGCAATAAAATTTCATAGGCAGAGGGAGAAGCAACTTTATCATGGCACAAGAATA
    ATAAGCAATATCAATACATGTTTCCAAAATAGAGGACGCTTTGGAGTCAAGTGGCCATATGAGGGAATAA
    ACTTTGCACAGAAGCAAAGAGGTTATTGCGGGAAACCACTGCACCGTGAGACTCCTTGAATGATGCCACT
    CTCTGAAGAGTCACTGGTGGAAGTCAAGAGATACAGTTGTAGAAGTGTGGTGCCAGGTTGCCTGTGACCT
    TCACAAACAACCTGCAGACTCTGACTGAGTATAGTAGGCAGGAAAAACCACGGGATCCTTAAACAGTGAT
    GCTGAGCAACAGTTGCACATGATAGGAATTCAATGCGGTATTTTAATAGTTCAACTATAAACACAGACCA
    GAAGTTTACTTCTCAGACAAACTGTGGCTCTAAAGCAACGAACAGCCCTTGTACTTGAATGAATTGTTTT
    ACAACTCCTGGCTATCCATGCTCACTCTTCAGGGAAGGAGAACCTGTCAGTTCTGAAAACGAGTTTAGCT
    GAATGCATGTGGAGGTTACAAGTTAATGATTAACTAGTAAAGTACAGGGCTGTTCTCTAACAACTAAGAC
    CCTTACATGCCCTCTCTCCATTTCTCCAACACACAGACCCCACAGAACCTCTACTGGGATGGCTTTTGAT
    TGTTGCCTTCTTGTCTGAATTTCCAAACATCTCCTGCATCCTATTTTCCTGCACTTTAACCTCCACACTG
    AAGCTAAACATTAATCTCTCCACAAACCTATTTTCGGTAATTTAGATATCTCAGCAGAGATTGAAATAGG
    AAAGATTTGTCTGAAAATTTCTTATAAGCCCTCTCCTCCTAAAGATCTCTTTTGTGTCTCCACCATTACC
    TTCGTATTTTTATAAAACTATTACTGTAAGAACCAAACAAGGAAAACCTGGAATGGGTTCCTACATGTTC
    TTGTTCCTGCAGACTGATAGCAAGACCCCTCATTCTTGATACCAAGCCCTACTGATAGTGATACCCAAGT
    TTCTTGTATATTAGGGGAGAATACTTGCTCATATTTTAAAACATCTTTAAATTACCCATACTAACAAATT
    CAGTATAAATGCTATAGAAATGAATACACTAATGGTCAAGAAAAAATAACAAAGAAAAAAATCTGTGCAT
    GGCCAGTGTGCTAGTTTGAATATGCTTGGCCTAGGGAGTGGCACTATTAGTGGCTGTGGGCTTGTTGGAA
    TAGGTGTGGCCTTATTGGAAGAAGTGTGTCACTGTGGGCGTGGGCTTTAAGATCTTCATCCTAGATGCCT
    GGAAGTTAGTCTTCTCCTGTTTGCCTTCAGATGAAAATGTAGAACTCTCAGCTCCTGCTGCACCATGTCT
    GCCTGGATGCTGCCATGCTCCCACCTTGATGATACTGGTCTGAACCTCTGAACCTGCAAGCCAGGCCCAG
    TTAAATGTCCTTAGAAGAGTTGCCTTGGTTATCATGTCTTTCACAGCAGTAAGACCCTAACTAAGACAGT
    CAAATTTTTCTGCCAAATCTATTTGATCTGTGATTATTGAATCATGGCGTTGAAAGGGCCAACATCCCAT
    AGCAGGTGGGAAGTGAGCCAGAAGTTCTTTAACTACTTGAGTAACTCTTTCCAGCTTCCTACAGGGATAA
    ACAACCAAGAACAGAGAGAATCTGTACTCCACACTAACAACAGTAGCTACACCACATTGTCCAAACCCCT
    AACTTCTCAGAACTTTCCAGTATTGACTTTGCACTCTGAATGGACCACTGTGCAGAAGAGAGGAGTCAGA
    CCCAGAACCTTATAGAGCCTCTATATGAAGCTGCACAACCTGTCAATGAGTCCTGATTCCAAAAAAGATA
    CTTCTGAGAAATATGTTCTAGCTAGAAATATGTGATTACGATGGCTTTGATCAAATGTTTGATGGGGAAA
    AGATAATTCTTGATAAAAAGAAATTACGTGGAGAACTCAATTACCAATATTTTCCATAGTTATACTGTCT
    AACAAGGCTGGAGAGACGGCTCAGCAGTTAAGAGCACTGGTTGTTCTTCTAGGGGTCCTGAGTTCAATTT
    TCAGCAACCACATGGTGACTCACAACCATCTATAATGGAATCTGATTCCCTCTTTTGGCACACAGGTGTA
    CATGTAGATAGAGCACTCATCTACATTAAAACAAACAAACAAACAAACAAACAAACAAATCCTGGGGCTT
    AGATTGTGTAATGAAAAAACTGACTCTAAGTTGTTCTCTGGCCCACAGTGACACACTTCTTCCAACAAGG
    CCATACCTACTCCACAGCTCCATAAGCTCCTGGGATACATATCCCATGGGCACATAAATAAATAAATATT
    GTCTAACAAAACTAGAAAAATTACCATTGTTAAATATTTCCCAGATTTTACATTTTCTTTTAAAAATCAT
    ATTATAATTGAAAATTTTTACTTTAAATTATATACAAGCATACATGAATGCAGTACCTGAAGAGTCCAGA
    AGATGGCATCAGATCCTGCAAAATTTAAGTTAAGGATGGTTATGAGTCACCTGACCTGGGTGTAGGGACC
    CGGATGTGGGTACTCTGCAAGAGCAGCAAGCACTCTCAGCTTCTGAGTCATCTCTCCAGCTTCCTCATTC
    CCCAAGACACACATCAAAATATTAGAAGTACATAAGCAAACAGCAAATCTGAGTATTGGTTTATTAGTAA
    CTTATATGTGGTGGTAATAAATAATCAGTTTTACTTTTGTTGGCATCTGAACAAATATTCAGACAACTAC
    AAGTTAGCAAAGTGTTTTTGAAAGGGAAAGTACACCCAAGAGCAAAGTCCCACATTAATAACCTCTTCCA
    TACAGAAGAGCAGTTTTATTGTTCCAATGCTGTTTAGAGCTGGAAAAAAAATGTCTGCAAGACTCATATC
    TTATTTGATTTTACTTCAATAAATTTTTATATTAAATTGAAGTTTGTGGACAGTACACTGCCAGCAGACA
    ACAATGACACTTGCCCTCAGCATCCACACAACTGATCGATGTTGGAGAATGGTTCTCTGCCTATAGAGAA
    ATCTCCTTGCACTAACAGCCCTCAGTCAAGCTCCAAATGACTTGGCTGGAGCGTGCATGGAAACACTGCA
    CGCATGAACAGCAAGAGTCTTCTGGAACAGTTATGGACCACTCAAGTGCTTGTCTTGTCTTGGGAGAAGT
    GATTTCGTAGCTTCCAATAGAGACAGACAACATAAAACTTATAAAAGGGTAAAAGACGTGGTACGAATCT
    GGTTGCCTCAGCTTAGCCCTTCAGTCACTCAGGACTGTGCCTGACTGAAATCTTATCAAGCAAATGACAA
    GAGGAGAAATGAAAATGTGTTTCCTGGCCATAGATAGCATTTGAGTGTCAGCACTGCTCATGGCATGGAT
    GTACAAGGAAACAGCTTGTTGGGGTCACTAATTACTCTTGACTTATTTTCTTATTTTTAAAGAGAAATGC
    CTCTGTTGTTGGGTTTCCTTTTGAACAGCAAGTGTCTTGGATGGTTTTATGTCACCTTGACATAAGCTAG
    GATTATCAGAGAGGAGGGAACCTCAGCTAAGCAAATACCTCCATAAGATCAGAATGTAAGCAAACCTGTA
    GAGCATTTTCTTAATGTGTGATTGATGGAGGAGGCCCCAGCCCAGTGTGAGAGGTGCCACCCATAGACTG
    GTGAACCATCGACTGTGTTCAATGAGAACACAGGATGAGCAAGCCATTAGCAGACCTCTTCTACGGCCTC
    TACATCAGTTCCCACCTTCAGGTTCCTGCCTGGCTTTGAGCTCCTGCCTTGGTTTCCCTCTGTGGACTGT
    GATTCGGGATATGTGAGCCAAAGAAACCCTTTCCTCTAAGAGTTTCTTTTGTTCATGATGTTTCATCCCA
    GAAAAAATAACCATAACTAAGACACCAAGCAATCTGTTCCCAGACCAAATGAAAGATTGCTAAGAATTAT
    GCTATCCTTTAAAAACGTTTCGGCTCCTTTTTGTAGAAGTTAATCAAACAGGCCAACTGCCAAGAAAAAC
    TGAAAGGAATAAAATGTTTCATGACTCATTTTATAGAAGAATTTAAAGCATCAATACAAAACTGTTTGAA
    GGAAGAAAAGTAAGGAGGGAGGGAGTAGGAGAGAGTAAGAAAGGAAGGTAAGCAGAGAGAGAGGGAAGGA
    AGGAAAAAGGAAGGGAAGGAGGGAGAGCAGAGAGTAAGAGAGGAAGGAAGGTAGAAAGGAAGGGAGGGAA
    GGAGAAAAGAAGGGAAGGAAGGAGAAAAGAAGGGAAGGAGGGAGGGAAGGAAGAAGTGAGGGAAAGAGGA
    AGGGAGAGGAAAGAAGCAAAAAGGAGAGAGGAAGAAAGGATGGAAGGTAGGAAGAGAGGGAAGGAAGGTA
    AAAGAAAGGAAAGGAGGGAGGGAGGAGGAGAGGAACTATGTTGTAAGGGATATAGACACTTGGTCATACA
    TCATTCAATTAGTATATAACATAACCACTATGTTGCTGAACTGTAGGACATCAGGTGTGCAGTGGCTCGT
    CCAGATATCACCAGTACCAGCATGTTAAGAGAGCAAAGCTGTGGCCATTCAAGGTCAAGAGAGGGTCTGT
    GAGATAGATCTGGGAGTGAGGGTGGAGCATGACTTTGTGTGTGAGAGACAGGGAGTGACAATGCAAGCAA
    GCCTGGGTCCGAGCTTAGGCATCTGGATCCACGTGCTTAACGGAACGGTTGTGGATTTTTTTTTTAAAGA
    AAGAAAATATTGGAACAAGAATTTGTAACATGGCAGAATTGAAATGAAAATATATTTGCCACAGTGAAAT
    AATCTCCAAACCACCTGATAGATATTGGGGACTGTTTCATAGGAAGCACGTCTCAGCAGCTAGGTCTGGA
    GTCCAAGGGGACATGTTAAATGATACGTGGTTATTGTCATTTCAAATGTCCAAGTAAGACACCCAAAAAG
    GCTTGGTTGGTTTCCACGTACAGGGAGGAGAGATGCATAGACTGTGTTGTTCTTTCCTACAAGAGGCTGG
    TGGGGGAACAGTTCAGAGAGAGAACAGTGGGTGCTAAGATGAAGGCTATGTCCAAATACCCAAGTCAAAC
    ACGCCCTCAGTAAAAATAGCATGGGGAGCTTTGTTAGTTCATCGGTAGCTAACAGGACGGCAACACATAA
    AGATGCTTTCCGGCCAAATCTTATGAAAGCTTACTTTATAGGCAAGGCATAAAGCCATCTCCAGGAGGCA
    GCTTTACGAGAGTGCTCTCCAGCAAGTGTCTGACAAAAGCAGCCATCTTACAAGTCTCAGATAGTTTGAA
    CAAAAGAAACAAAACCAACCGGCAACATAATATTTACCCCATAAACGCAAGCTCAGAGACAGGGAGGGCA
    CACTTCTGAGCATGGGACTGGTGGCCTGTCACTCCCTGTCTCTCACAAACAGAGTCATGCTCCTCCCTCC
    AGCTACAATAAGGAACTGCTATCAAACTGGAAAACCAACACATCGGCTTCCAAAACACATGGCCTCCATG
    GTCCCCCCTAACAGAACCCTACAGCTTTATGAAGCCACGCACTGACAGAACAGCAATGGAAACAGAAAAT
    AAAATCAAAATGAAAACATAAACAGCTGTTTCCCTTGCACCGTGGCAACACTGCAAATATATAGCAGTAT
    ACGTGCCTGCCTGGTGGAGGCTTCTTTTGAATTTTTTGGAATGCCAAGTGGCAAGGCAAATAGATTCACT
    CTCTGTTGGTTGTTATTGTCCTAATTTTATTGCCAAGAAAAAACAATCTTTCCCTATGTGCATGTTACCC
    TTAATATCCTCTCTTCCGGTGAGAGAAGTTATAGCTTAGGCCCAATTTATAATTTTACTGTTCATTTAAT
    TTCCTTTAACACTTTAAAATGTTATGTGAAGGCTCTGACTTGTCAAAACTGAGCCATCCATTTCAGGCAT
    AACCCATCAAAATGACAACACGAACAGTTCTCATAATCTACAACAGTGAAAAAGGCAGACCCAATGCTTT
    AAAAGAGAGAGTTTGTCCACACCAACTACTCTGCACTATCATGTTTGACATTCGTGTGTTATTTTAATAA
    TCTGTGCTGGAGAGCAGAAATATTTGTGCCTCATTTAAAATGCTATCAAGTATAAAGTTTTTTTGTTTTT
    GTTTTTTAAGTAGCATTTGAGATAAATATATTTCTGGCCCAAAGAATAAAGAAAGATGTCTTTGAGAATA
    CAAAAGCCTTTATTTCATAAGAAAAAAAATTATCTCTAAAGCTTCCCCACAGCCGGTCCTCCAGACTTTC
    CACCAGGCTTTGCTTGAACATGGACGAAAGTACACACACCTGGAATTCCTTTTTCCTCTTTTTACAAGAT
    CCAGGAAGTGAATTCTTATTAGTCAAACCCAACTCAAACACATCATAAATGATACTTGTATCATTTATGG
    AATTTGGGAACTATGCATATTTATAAGCATGAATGCTGCAGCCCAGTGATGTCTATTGGCTTGCCCCTAG
    TGAGTTATCAAGTAATCGAGCTTTAAAGCTGTTTAGCCCATAATCCACACCATTCAAAACACACAGAAAG
    TGGTTACAGTCTGTCAATGGACTCTCTGGCCTGGTGAATACCAGGAGAAAAGGTGACTCCCTGGGTATTC
    AGCATAGAGACTAAGAAGCTGTCAGTTAAGAAGGCAATGGTTCTATCAAAGTGTTATATTCTACTGCCCA
    AGAGATGCGCGCCATCTTTATCCTATTTAAAATCAGTAGAACAACGCTTGTATCAGCTTCTCCTTTGTGG
    GGACATTAAATCTGTTCATGTAAATGTTTGTGATCTCTAGTTAATTATCTGTGGGTATTTTATTATCCTG
    GGTATAATTTCCCTTTTCTCTTCTATACCTTTGTTTTCTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
    GTGTTTTAAGACAGGGTCTCATTCTGTAGCCATGCTAGCCTGTAACTCACTAAGAAGCTTAAACTAAGGG
    CAACCCTCCTGACTCAGGTGCCCAAGTCCTTGGATTACAAGCATGAGCCATGATGCCTCCTTGGCTCTTT
    CTCAGTTGATTTTGCATCTTTTGCAGCTCCCCTGATGCCTTATTATGAGACTCAAAAACATTTAACACAA
    TTAACTGGGAAAAAAGGCAAGTAAGTAGAAAAACTGTAATTTCCATGGGTATTTGGACATGATGCTAGTT
    AACCAAATTTCTTCAGTGGGTTTATGAGACAGAGGGGAGAAGGCAGCATCAAAGCAGGTGATAAAATAGG
    GCAGATTACACTAACAAACACAAGCACAAATCAAAAATTATGTAGAGCATAACGAAGCATCATTTATGCA
    CTGCTTGACCATCTGGACCTGCTGTTTTATGGGACAAGGGGGTGAGAACTTGTGCTTGAGCCTTCTGAGG
    TGGTACTTTTTATAGCAGTATTTGAGACCCACACAGACTGGCTGTACAATGATATGGCTGCTTTACAGAA
    GGCCCATCCATAGAAATCAAGCCCTGCAATGAATGCTGTGTAAGGACAAAGCCCCAGAGTTCAGTCGGCT
    GCAGACTTGCTGACATTAATTCTGCCAATTCCTTGTGATTCATAGATTTATGCTTCTCCCTTCAAGTGGG
    AGTATGACAGGGGGAGGACGCGTGTTGAGATATCGTCTCTGTGCTGTGGTGGGTCACTTAGGACTATGTT
    GTGTCATACTGAATGGCTTACCAGAGAAGTTGCTGAGCACAAACATCAAATATGACATTAGTTATAAAGA
    AGAGAGGAAGAGGGAGAGGGAGGGGTGGGGGAGGGGAGGGAGGGAGAGAGAGAGAGAGAGAGAGAGAGAG
    AGAGAGAGAGAGGGTCGAAAAGGGGAAACGACTGACAGAAATTATGAGGAAAAATTAGACTTAAAATTAC
    ATATACTATATAAATCACCAACAGTTTGAAAAGGATGAGAGCTATGAAACACAGCATCCATATTTCCAGC
    ATGATAAGCAGCTTTTGCTGATAGCCAGTGAGGAAATATCAATCTCATTCCTAACCTACAAAGACTGAAT
    TCTGACAACAAACTGTAATGAGCCAGGCAATGGATTCCTTCCAGCATCTCCAGATAAGAGCTTCCCCGGT
    AAATGTCTTGCTTTCTACCTAATGAGACTCTGGAAAAAATATCCAATTGAACTTGACACCCATGTAAACT
    GTGACTCTATTGAAGATGTTGTTTTAAGCCTCTTTGTTCATGACTTCTTACACGGCAATAGAAAGGTAAT
    TTATAGAGCAATGTGTTTCACTGCAGGCATTGTGGAAAATAGATATAAACTAAATATTATGTTTATCTAT
    TTTATAAATAAATATATACAATACATATATTATATAGTTGCCTTAAATGATTTTAATAGGAAGCAATGAG
    ACTACATATGCAATCAATCTAGAGCAGGTTTTTGGGTAATAGTTATTTTTAACGTGTTTTTCTTTCATCT
    TTGGGGTTATGTCATTTATCCTTTCCCATCCTCCCTCAGCCCTTCTATACACTCCTCCTGCTCTCTCTCA
    AAGTTATGGCCTTTTTTCATTAATTGTCATTGCAGGCATACGGGGGGGGGGGAGGGAGGGAGGGGGAGAG
    AGAGAGAGAGAGACAGAGAGACAGAGAGACAGACAGACACACACACACACAAACAAACACACAGATATAT
    ATTACAAAATATAACCTACTCAGTCTGTATAATGTTACTTGTATGTATGTCTTCATTACTAACCATCTGG
    TATTGGATAACCATTATTGTGCTCTTACCTGGAGAAGCCTATTGCTGCTACCCCAGCTTTCCCTATGGTG
    ATGAGGAAATATATCAAAGGCTTAAGGGAAGACTTTGGTGACTATGGTTGCCTGATAGTCTAGGGAATTG
    TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTCAAAATTGTTCCCTTG
    GTTGTATAACCTGAGAAGTGACCTTATGAGTGTTGCTCTCCAAAAGGCTATGTTATATATTATGAGGCAG
    AACACTTCATAAATTGCATGTGCACACACACACACACACACACACACACACACACACAATCATTGCATGC
    TTCTTGTTTTCAATGCCCAAATTCATCCGTACTGATAAGAAAGAGGGATTCTACAGTACCATGTGCTTAC
    ATGTGAATATGTAAAAGAAGGAGGGTAACTTTACTACAATTTATTCCAGATAAAATTAAACATCAATTGC
    CCACTGCAGTGCTCTAACAACAGTGGGAGGAAATGATACAGTCATGAACAAAGACAAGGTCATATTTGTC
    TTCTCTGAGCTAGCGCTCTACAGAAGAGAGATGAGCCTAGCAACTGCAGATAGTAGTCTATATTAGAGGA
    AGGTAATGTGCTAGCATATAGAGGAAGAGGTTTCGCTGGGGGAGGCTTTCTGCAAGAAGCAATGGGAGTG
    GACCTAAGATCTTCAGGGCGAGAAAACACTTATTGAGAGGGTTTCAAGCAAAAGTAAACTAATCATTGGC
    CTTGTTATAGGGGCAGGAAAGTGACAGCAACCTAGAAACAAAGTTTACTAATGCTTACTAACACTGAAAA
    TGGTGTAATGATCAGGACTTTGAAATGCTCAACCTGCTGGACTTTCTCACAGGTGTAAACCCTTCCCTCA
    TGACACACCAGACAGTAGCTAACCAAAAACTCGTGCCAGCAGCAACTGCAAGCAGACTGAGCTGAAACTT
    GCTGGCCTGCTTTCCTTATTATTATCTACTACTTGTGGTTACGAAATAGGAAGAATACAATGGTTGACCA
    TCACAAATAAAAATGAATTTACCCTTTACTACATGGTAAATTAAAACCTGCCCCACATGTATGTGGGCTA
    AGACAGTCACAACAATCTTACTTATTTTGTGTGCCTTTCCAAAGTCCCTTTACTGATGTGGGGTTTCTAC
    TTTTCTCCTCAAATCAGCACCAAGTCCTGACCATACATGCGTAGTTATGCCTGAAAATATGTGTGAGTTC
    CACGGCTAGGACTTAAAAGGTCTGTCCTCTCTGTTCCTTCTACTGGCACACAAGAAAACTCAGCGAGACA
    ATAATGATTGAAAACAGTATAGACGTTTATACCTGCCATCCCAAGACACGGAAATGAAAATAAATCAAGG
    AATCAATCGATAAAACTATGAGGAATAACAAAGTATATCTAAAATAACTCTATGTGACCTAAACTGAGCA
    TCTATAGATACAAAACTATGTACTATAAATGTTATTAATTTATCTGAAATAAAATATTGTCTTTAAAAAC
    TTCTGGGTTGTAATTGGGGAAATTTTGATCTCTATAAATATCTCTTGAAAATAAACCTATCCTGGCTAGC
    CTTATAATTAATAAAATCATGCCAACAAAATATATTTAATCTCTAATATCTATGTGATCAAGGGAACAGG
    ATGGACTCATTATTCATGGCTAGTTTAAGAAAGTCCTTGGTTAGAATGGACAGAATACCAGAACTCAGAT
    TTTCTTCATTTGAATATTATCTAAGATGTATTTGCTTTGGTCAATATTTAAAGAATGTAACAATTTCACT
    TTCCATATGCCAAATCATCACAAAGTGTTGGTGTCAAACTGTTTCCTAAAGCTAGTATAATTAATTTTGT
    CTTATACTATAAAACTCTTGTAAATCAAAGGTATCACTCTTTGTTCTTGCAATATCTAGGAAGTCAAGAA
    TTATTCATAATTACTTCCATGGACCTATCAAATTAGGAATTTTCTGATATAATTTTGTTAAAATAAAATC
    AACATTTAAAAAGTCTGGATTTTGTTTAACTTACTTTATAGTTGATTGACAGACGCCTTTAAAAGATGCT
    TCAAGGTGCTGACAAGTTAAGGATAATTGAGAGTCTTCTTCTGGACAGCCTTGTGAGAGAAGCTAGCAGT
    GGCTATTGAATGCTCATCCCTGAATGGAACATCTACATTACACCCACCAAGGCCCACGATGGTAACCAAG
    AAAGGATGGAAAGGCTGTAAAAGCCAGATGCAAGGAAGGAATGCTGAGAAGAGTGGTGTCTTCTGGACAT
    GGTCTGGCTGTCACTGTCATGGACACATAGTGTCTGTGGCTATGTGCTCAAGAAAAACATGAAAGCAGAA
    ACATGGATGGTTGAAAAGAAAAAGGCCTGTGTGTGAGTAAGAAAGGCATTAGAGAATGTATTGGGGAATG
    AATATGATCACAATAAATCGATGGTGTGCATGTAATTGTCAAAATGAATAAAATTTTCTTTCAACTTACA
    AATATTTATATACTTTAGTGATAACAGTCAATTTTAATATTTTCATGCTATAATTCAACTTCTGTTACTG
    TGTGGTTACAGCAAAGATCATTTTCTCAGTCCCAACATACAGGTATTTCTTCCCATCCCTGAAACCAAGC
    AACTCCACAGGAGACACCAGTTAAGTATTTGATAATTCATTATACGCCTAAGGTTGCCTCTTTGAGAAAG
    TGTCAGACCTGGTGTATTGGTGGCTTTGTTCCACAACTGTACCCCACTTCTGACACCTGAGACACGTCTG
    CCCTCTGGAATTCCTGCTGGACTGCCTGCAGTTAGAGCCCAGCATCCTTTGTTAGCCCTATTAAATTGCT
    ACAGCATTATTCAAGATGCCATGGGAACACATTTACTAGTTTGTTGAGAAGGATATTTTTAAAGAACAAA
    CAACCATTTGAGCTACTATGATAAGGCCTGGTAGGGGCCAGACACAGGAGCTTCCATCCCTGTGTAACTG
    ATATGTGCAACTCTCCTGGAGTGAGAACAATTCTCAGCTTGCACATGTTCTAGAAAGTTCTCTGAACCCA
    TCTCTATGGCTATTTTAAAGGAGATTTGTTTACATAAGCAAGTCTGGAAAACAGGAACAGCATGGAAATT
    GCTAACATACTAGATGAGACCTACCATACTAGGTGAGACCTACCTACGCTGTTGCACCCAAGGCTTCTTG
    TCCTGCTGAGTAATACTCTTCCTGTTGGCTAACTCACAAGATTCTTGCTAAAATGAGATTTTGTGAATTA
    CAATCAAGTAAAGCAGGCAAGAGACCTCCTCTAAGACCAACTTCAAGAAGACAGTGGAATAGAGAAGCTG
    GAAGCTGTTTTTAGTTGCCATGATGTGAATTGGGGAAGAGGAAAGAGGTGAGACTATCTTTAGATCTATG
    ATTGCCATTTAGGAAGAGAGAAGGGAATGGGGAAAGGAAGGGCAGGACATGTTAGGGAGCAGTTGCAGTC
    AGAAGCACTCCACCAGCAGAGCAGCCAAACCTGCCTACATTGCCTCCACATGGCAGCAAACATCAATGCC
    AAGTTATCTAGTAACCACAGTAACCTCACTTTTTAATTCTCCTCTGTACAGAGACTAATCTAGATCCTGA
    GATGTACCATCTCAGTCAAGGACCTGATCTCCTACAGTGGATACAGTAGATGACAAATCGTGGTTTCCTG
    TGCCAAATTAGGTCAAGGTCATTTACCTTGTTTTGTTCCACATTCTTATTTCTGAATTACACTTAAGTAT
    AAAATTACATCATATATATATATATATACTTATATTTTAAATATAAAATCACAATATTTAATGTTAACAT
    ATGTATTAAATATGCTACTTTTACTTTTAAATATATATATAATTTATTTTAAACTATATATAATGTATTT
    TACTTTAAATTATACTCACAACTTAAAAAGTAGTACATATATATATATATATATATATATATACACACAC
    ACACACATACATATATATTAAAATGTATACATTAAATGTGAATATGAAGAATGACTCTTTAAGGTGACTT
    TCTAAGGCAGGTCCACTCAGGAGTGCACAGGCTACAGAAGCAGAAAAACTTCTGGAACAGGGTCCTACAG
    TGTTGCTCTGCACCTGGGAGATGGGGCTGTTCCGCAGCCCTCTGTATACTGGTCTTGTAGGGAGAGAGCT
    GGTCTCCTAAGAGTGCTGACACGGGCTTAAAGACTCACAGGAGGAACAAGCTCCAGGCAGAGACAGCAAG
    AACATCTAACACCAGAGATTACCAGATGGTGAAAGGCAAACACAAGAATATTACCAACAGAAATTAAGAC
    TACTTGGCATCATTAGAACCCAGTACTCCCAACAAAGCAAGTCCTCGATGCCCCAACACACCAGAAAAAC
    AAGATTTAGGCTTAAAATCATACCTCATGGTGTTGGTAGAGGATTTTAAGAAGGACATTAATAACTCCCT
    TAAAGAAATACAGGAGAACACAACTAAACAGGTAGAAGCTCTTAAAGAGGAAACACAAAAGTCTCTTAAA
    GAATTACAGGAAAACACAACTAAACAGGTGAAGGAATTGAACAAAACCATCCAAGATCTAAACATGGAAG
    TAGAAACAATAAAGAAATCACAAAGGGAGACATCTCTGGAGATAGAAAACCTAGGAAAGAAATCAGGAGC
    CATAGATCACCAACAGAATACAAGAGATGGAAGAGAGAATCTCAGATGCTGAAAGTTCCATAGAAAACGT
    TGACACAACCATCAATGAAAATGCAAAATGATCCTAACTCAAAACATCCGGGAAAGCCAGGACACAATGA
    GAAGAACAAACCTAAGGATAATAGGTTTAGATGAGAAGGAAGAATTTCACTTTAAAGGGCCAGCAAATAT
    CTTCAACAAAATTATAAAAGAAAACTTTCCTAACCTAAAGAAAGAAATGCTCATGAACATACAAAAAGCC
    TACAGAACTCCAAATAGACTAGAACAGAAAAGAAATTCTTTCTGACACATAATAATGAAAACACCAAATG
    CACTAAACAAAGATAGAAAGATAGCAGTAAGGGAAAAGGGTCAAGTAACATATAAAGGCAGGCCTATTAG
    AATTGCACCAGACTTCTCACCAGAGACTCTGAGAGCCAGAAGATCCTGGACAGATCTCATACAGACACTA
    AGAGACCACAAATGCCAGCCTAGTCTACAATATCCAGCAAAACTCTCAATTATCAAAGATGGAGAAACCA
    AAGCATCCCATGACAAAACCAAATTTACAAAATAACTTTCCACAAATCCAGCCATTCAAAGGATAATAAA
    GGGAAAATTCCAACACAAGGAGGGAAACTATGCCCTAGAGAAAGCAAGAAAGTAATCTTTCAACAAACCT
    AAAAGAAGATAGACACATGAACAGAATCCCAAATGTAACAACAAAATTAACAGCAAGCAACAATTACTTT
    TCCTTAATATCTCTTAATATCCATGGACTCAATTCCCCAATAAAAAGACATAGAATAACAGATTGGCTAT
    GTAAACTGGACCCAACATTTTACTGCATACAGGAAACCCACCTCAGAGACAAAGACAGATACAACCTCAG
    AGTAAAAGGCTGGAAAACAATTTTCCAAGCAAATGGTCCCAAGAAACAAGCTGGAGTAGCCATTCTAATA
    TCGAATAAAATCAATTTTCAACCTAAAGTTATCAAAAAAGACAAGGAGGAACACTTCATGCTGATCAAAG
    GTAAAATCTACCAAGACGAACTCTCAATTCTGAACATCTGAGCTCCAAATGCAAGGGCATCCAAATTCAT
    TAAAGAAACTTTAGTAAAACTCAAAGCACACAGTGCACCACACACAATAATAGCAGGAGACTTCCACACC
    CCACTCTCATAAATGGATAAATCATGGAAACAGAAACTAAACAGAGACACAATGAAACTAACAGAAGTTA
    TGAACCAAAGGGATTTAATAGATATCTATAGAACATTTTATCCTAATACAAAAGGATATACCTTCTTCTC
    AGCACCTCATGGCACCATCTCCAAAACTGACCATATACTCAGTCAAAATACAGGCCTCAACAAATATAAA
    AATGTTGAAGTAATCCCATGTATCCTATGAGATAACCACAGACTAAGGTGATCTTCAATAACAACATAAA
    TATTAAAAAGCCTCCATACACATGAAAGCTGAACAACACTCTACTCAATGATAACTTGGTCAAGGAAGAA
    ATAAAGAAATTAAAGAATTTTTATAGTTTGATGAAAATGAAGCCACAACATAACCAAACGTAAGGGACAC
    AATGAAAGCATTCATGAGAGGAAAACTCATAACTCTGAATAACTTTAAAAAGAATCTGGGGTGAGCATAC
    AATAGCAGCTTGACAGCATACCTAAAAGCTCTAGAACAAAAGGAAGCAAATTTACCCAAGAGGAGTAGAT
    AGATAAACCCTTAGCTAGACTAGAGGGCACAGGGAGAGTGTTCTAATTAATACAATCAGATATGAAAATG
    GAGACATAACAATAGAATCTGAGGAAATAAAAAAATCATCAGATCCTACTACAAAAGCCTATACTCAACA
    AAACTGGAAAACCTGGATAAAATGCACAATTTTCTAGACAGATACCAGTTGCCAAAGTTAAATTAGGACC
    AGATTAGTGATCTAAACAGTCCTATGTCCTCTAAAGAAATAGAGATGGTCATTAATAGTCTCCCAAACAA
    AAAAAGCCCAGGACCAAAGCAGTTTAGTACAGAGTACTATCAAACCTTCAAAGGAGAGCTAATTCCAATA
    CTTCTCAAACTATTCCACAAAATAGACACAGAAGGTACTCTACCCATTCATTCAAAGAAGCTACAATTAC
    TCTGATACCTAAACCACACAAAGACCCAACAAAGGACAACTTCAGACCAATTTCCCTTATGAATATTGAC
    GCAGAAATACTCAATAAAATTCTCACAAACCAAAACCAGAACACATCAAAACAATCATTCATCATGATCA
    AGTAGGCTTCATCCCAGGGATACAGGGATGGCTCAACCCACTATATAAACAAACTCAAAGAAAAATTCCA
    CATGATCATCTCATTAGAGGCCGAGAAAGCATTTAACATAAGCCAACACCTGTTCATGATAAAAGTCATG
    GATAGGTCAGGAATTCAAGGCATATACCGAAACATAATAAAATCAATATACTGCAATCCAGTAGCCAACA
    TCAAACTAAATGGAGAGAAACTTGAAGCAATCCCACTAAAATCAGGGATTGGACAAGTCTGCCCACTCTC
    TCTGTACCTATTCAATATAGTACTTGAAGTCCTAGCCAAAGCAATTAGACAATGAAAGGCGATCAAAGGG
    ATATAAATTGGAAAGGAAGAAATCAAAATATCACTATTTACAGATGACATGATAGTATATATTAGACACC
    CCCAAAATTCCATATGAGAACTGCTAATCCTGATAAACAAATTTAGTGACATATATGGATATAACTCAAA
    CAAATCAGTAGTCGTTCTCTACACAAAGGATAAGGCTGAGAAAAGAAATTAGAGAAACAACATCTTTTAC
    AATAGTCACAAATAATATAAAATACCTTAGTGTGACTCTAACTAAGCAAGTGAAAGGTCTGTATGATAAG
    AACTTCAAGTCTCTGAAGGAAGAAATCAAAGAAGATCTCAGAAGATGGTAAGATCTCCCATGCTCATGAA
    TTGGCAGGATTAATATAGTCAAAATGTATATCTTACTAAAAGCAATCTACAGATTCAATGCAATCACCAT
    CAAAATTCCAACTCATTTCTTCAGATTTAGAAAGGGCAATTTGCAAGTTCATCTGGAATAACAAAAAACC
    TAGGACAGCAAAACTATTCTCAACAATAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCTGGTGGAATCAC
    CATCCCTGACCTCGAGCTGTACTACAGAGCAATTGTGATAAAAACTGCATGGTACTGGTACAGTGACAGA
    CAGGTAGATCAATGGAATAGAATTAAAGACCCAGAAATGGACCCATATACCTATGGTCACTTGATCTTTG
    ACAAAGGAGCTAAAACCATCCAGTAGAAAAAAGACAGCCTTTTCAACAAATGGTGCTGGCTCAACTGGCA
    GTTATCATGTAGAAGAATTCAAATTGATCCATTCTTATCTCCTGTACAAAGCTCAAGTCTAAGTGGATCA
    AGGACCTCCACATAAAACCAGAGACACTGAAACTTATAGAGGAGAAAGTGTGGAAGAGCCTCGAACATAT
    GGGCACATAAGAAAAATTCCTGAACAGAACACCAATGGCTTGTGCTGTAAGATCAATAATTAACAAATGG
    GACCTCATAAAATTACAAAACTTCTCTAAGGCAACGGACACTGTCAACAAGACAAAAAGGCCAACAAAAG
    ATTGGGAAAGGATCTTTACCAATCCTACATCAGATAGGGGAGTATTATCCAATATACACAAAGATCTCAA
    GAATTTAGACTCCAGAAAGCCAAATAACCCTATTAAAAATGTGGTACAGAGCTAAACAAAGAATTCTCAA
    CTGAGGAATACTAAATGGCTAAAACTGCTCAATATCTTTAATCATCAGGGAAATGCAAATCAAAACAAAC
    CTGAGATTCCACCTCACACCAGTCAGAATGGTTAAGATCAAAAACTCAGGTGACAGTAGATGCTGGCCTG
    GATGCGGGGAAAGGGGAACACTCCTCCATTGCTGGTGGGATTGCCAGCTGGTACAACCTCTCTGGAAATC
    ATTTTGGCAATTCCTCAGAAAATTGAACATAGTTCTACTGGAAGATCCAGGAATACCACTCCTGGGCATG
    TACCCAGAAGATGCCCCAACCTGTAATAAGGACACATCCTCCACTATGTTCATAGCAGCCTTATTTACAA
    TAGTCAGAAATTGGAAAGAACCCAGATGTCCCTCAACAGAGGAATGAATACAGAAAATCTGGTACATATA
    CCTATTGGAGTACTATGCAGCTATTTAAAACAATGAATTTATAAAATTTATAGACAAATGGATGGATCTG
    GAGGATATCATCCTAAGTGAGGTAACCCAGTCACAAAAGAACACACATGATATGAAATCACTGATAAGTG
    GATATTAGCCCAGAAGCTCAGAATACCCAAGATACAATTTGCAAACACATGAAACTCAAAAAGAAGGAAG
    ACCAAAGTGTGGATATTTCGATCCTTCTTAGAAAGGGAAACAAAAAAGGGAAGGAATTGCGGAGACAAAG
    TTCGGAGCAGAGACTGAAGGAATGACCATCTAGAGACTGCCCTACCTGTGTACCATAAACAACCATCAAA
    CCCAGGAACTATTGCAGATGTCAACAAGATTTTGCTGACAGGAGCCTGATATAGCTGTCTTCTGAGAGGC
    TCTGCCAGTGCCTGGCAAATACAGAAGTGGATGCTCACAGTCATCCATTGGATGCAGCACCGGGTTCCCA
    ATAAGGAGCTAGAGAAAGTACCCAAGGATCTGAAGAGGTCTGCAACCCTATAGGAGGAACAACAATATGA
    ACTAACCAGTACCCCCTCCCCCCAAGCTCCCTGGGACTAAACCACCAATCAAAGGAAACACAAGGTAGGA
    CTCATGGCTCTTAGCTGCATATGTACCAAAGGATGGCTCATCAATGGGAGGCGATTCCCTTGGTCCTGTG
    AAGGTTCTATGCCACAGTATAGGAGAATGCCAGGGCCAGGAATCAGGGTTGGAGTGCAGGGGGCATGGGA
    GGGGATAGGGGAATTTTGGAGGGAAAACTAGGAAAGGGAATAACATTTGAAATGTAAATAAAGAAAATAC
    CTAATAAAAAAATTAAAATAAAAAAGAATGAAAAACAAAACACAAAGCACATATATTAAATCTGCAAACA
    TTAAATATTACTCTCTGATCCTTTACTTCCAAAAGGTTTGTAATTTCCTGACTTTGAAAGAATTCTAATT
    GGATAACCCACTACTATCTCAAATACAATAAATCTAAAGTAAAAATATTTTCTCAGATACAGCACTCTCA
    TCCTGCAACTCTTCATCTCTCCTAAAGCCAACAGAGTACAGGAATTCATCACCCATAAGTACCCTAGGCT
    GGAGACTATTTGCTCTCAGATCAACTATTCTTACCTATCTTGTCACCATGATACTGGAAGCCACACAGCC
    TGAACTGAGCACATCTTTCCATTGGATCCATGCAACTGTCTGTTGCTCATACACATGCTTATGCTGTGTG
    CTCCTGGTTGTGGGTTTCAATCAGCATCTCTTAGTTAAGTAATGACAGTAATATGTTCAGGTCTTCTTGC
    TAATCTTTGATTCTAATGCACTTTCAAAGTCTTGCTCAATTCTGCAACTTATTTCCAGCTTCATTTCCAT
    TTAGTTTCCCTCAGGAAGACACTTGTGCCCTTCACTTTGGATTTAAGAATGATGTAGCCATATCCTCTTT
    GCATTTTGCATCTTCAAATATAAAAATCAGAGTATTTTGCTATTACTGATGACATGAATAATAGGTAAGC
    ATTATCAAGACTTCTAATCTTATGTTTATATGTTACTAATTTCTGAATCACAGCAACATTGATAGGAAAC
    ATTATCATCATGTTAAATGTAATAAAGCTTCAGTACAAAGAAGTTGCATGTTGTCTTATACGATTGACAT
    GATAAAATGCCATAACCCAGACAACTTGGGAAAGAGAGGGTTTATTTTGCTTACACTTCCACACCACAAT
    TCATCACTGAAGAAAGCAGGACAAGAACTCAAAATGGGCAGGGACCTAAAGACAGGAGCTGATGCAGAGG
    TCATGGAGGGGTGCTGCTTAATGGCTTGCTCCCTATGCCTTGCTCAGCCTGCCTTCTAAGAGAACCCAGG
    ACCACCAGTTCAGGAGTGAACTCACCCACAATGAGCTGAGCCATCCCCCATCAGTCACTAAGAAAATGTC
    CTACAGGCTTCCCTACAGCCCTATTTTATGGAGGCATCCTTTCAATCAAGAATCTCTTCTCTCAGATGGC
    CTTACCTTGTTGACACAAGGTATCTTACTGCACAAGTGCCCAGATGATAGCAGCACCAGAACAGAAAGTC
    AGGAAGCCTCTTTTGAGAATTAAGCCAGGGGTATCTCCAATGTTAACTGTCCCCACTACTTGGCTTCTTA
    AGGATGCCACCTTGGAGATGAAATGTGGTCTAAATCTGGTTTCTCCATACGAAGAGAAGAAATTCAATCG
    GTGAAGACTCACTATGAGGATGACCTACATTCTGATATTCTTCCAGGAGAGAGGGACAACAAATTAAAAC
    AAAGCAGAAGCCACCATGCATGGACTGACTTCTCATTCCAGGGCTGGTACCCATGGGAAGGGAGTAGAAC
    ATCATTGGTAGTGAACTGAGTACGCTGTATCCAGAATTACCAACACAAAAGCCATCCCAGTCTGGAAGCA
    ACCATGTGACAGTGTGATAAGGATGAGAGTTAGTCAGGCAAAGAAGGACTGAGTTAACAGACACAAACCT
    CCTCTCTGGAAGATAAAGAGATTTCTGCCAAAACTCACAGGGAACTTGAAAGAGAGCTGAGGCGTTTGTA
    CTGGAGCAGCAGTTGCAAGATTGCCTAAAGGAAGCTGAACCCAGCCAGCATGTCAGAGAAAATGTATCCA
    TGTAGCTTGGGAGACAGACAGGGTCAGGAGAGCACCAGTGTGTCCTCACATAAAGCCCTCCATTCAGAAG
    ACAAGGGTATGCATATGTTCTAGGTTTGTGTGACATTAATGCAGAAGCAATCATTAATAAAATTACAAAA
    TGTTGTCAACACAATTATATAATATGTATTAATAAAGAGAATTTGTTCCCTCTACAATCTTTCTGTGCTC
    AAATTATATTTCTGGTACAATGGCTTTACTAACTATGACAATAGACAGAGCTTAATGTCTCATAATAAAA
    ATGTTAACAATGCCAGACTATAGAATATTAACATAGCCCGTAGTACCAGAAAATTTAAATGAAGAGATTA
    AATAAATAAGTTCATTAAGGGTTGATGGTAATAATAGTTCTACATGTCCATACAGATCTGAGTAAACTCC
    AGAGTGTTCTAAGAGTGTGTTCTATGCATTTTAAGAATAAGAGACAAATGAACACACTCACATTATCAAG
    CACTCCTAGAGAATTTAACAAAACGGACCCAGTGTTTCAAAAACTCCCTGTTTGATTAAATTCCACCCTT
    CGTTCCTCCAACAGAAGAAACAGAGCCCTCAAAAGCAGAAGACCATCTTGTTCTTTCATTTTTTTTCCTG
    ACTGAACACAATGCATCCTCTTTTAAAAGACACATATCCTGTGAGAACAGAGCAGGAGTAAGGTAAATCA
    TGGATGGAGAGATGGAAGTTCTCACGGTGAGCCACTGTCAAAGTTCCAGGCAGGCTTGCTCTCACTCTTC
    ATCCCTTTTGCATTGCCCCATAGATAAGCACAGGTATCTCAAGAGATAATAATACATACACCATGGCTTT
    TCTTTTTAAGCATCTGCTACAAGTGCCATACAAGCTGTGTTACAGGCTATGGATCAGTCACGTGCCTAGG
    ATATTTATGAGGCACATCTAATAATAGTCTCCATTTTACAAGTAAAAATAAGAGCACAGGAAGCTTGTGT
    GAGGTTGTAATAAAACCACTAAATGGCACGTCTGGGATCTAAATTCCACTCCATTCAATACTAAACACAT
    AGAGGTTTATGCAAATATAGCCACCACTATTTATAAGGAGAAAACACAATAGCCATGACTTCTGAAATGA
    GGCAATTGATAATGCTCAACAAACACCAGAGGTGCCACAAAGATTGAAGTGTCAACATCTATGATATGTT
    GGGGGAAATGTCAATAATAAATAAAAGCTACATAACCAACATCTTTCTTATGTATTGTTCTATACCCCAG
    CACTATGCAGGAGAAGTAGGTTATAGAGTTTTAGGAAAGAGTATAATAATGAACACACTGGCATTACATA
    GAGAAAACAAGTGTAGATTGGAAGACAGGTATGCAGTTAAAAAATTTCAGGTGTGGACTTAATCCTTAAA
    TAGAAATACTACATTACAAAAATGTCACTTTTTTTTCCAAATTTGTTCCCAAGAAATGAAATTGAAAGCA
    ACGTTCACATACAAGAGCTGATTAAGGAAGTGAAATCGATGCCGTGCTCTTAAGTTCATCTAGAAAATAA
    TCCATGGGTATAAGCACTATTTAAATAGTAAAAGAATAAGAGGAAGCAGACAGAGTGGAGCTTACAGTCC
    ACTAGTTTATGAAAAGAACATGGTTCACGAGTGCTGCAATACGTCTGTCTAGGAAACCTTCTAAATCTGC
    ATCTTGAAGCAGCAGCTTGGCCGAACCACTTAGCACAGCACATGAAAGAATTAACCCACTTTTTTCAGAG
    AAAGCATAGGGTCTTAGCTTATATCATGTGACAAACTGCAAACTGCAGATTTTCAAACAAAGCATCCAGA
    GGAAACATGTCTGATGTCTAGCTAGAGATACAGAGTTGATATTTTTAATTCAAAATGGGAAGAATAGCAG
    ATTTGATACAATTTCCTAAACACATGAAACTCAAGAAAAATGAAGACTGAAGTGTGGACACTATCCCCCT
    CTTTAGAAGTGGGAACAAAACACCCATGGAAGGAGTTACAGAGACAAAGTTTGGAGCTGAGATGAAAGGA
    TGGACCATGTAGAGACTGCCATATCCAGGGATCCACCCCATAATCAGCATCCAAACGCTGACACCATTGC
    ATACACTAGCAAGATCTTATCGAAAGGACCCAGATGTAGCTGTCTCTTGTGAGACTATGCCGGGGCCTAG
    CAAACACAGAAGTGGATGCTCACAGTCAGCTAATGGATGGATCACAGGGCTCCCAATGGAGGAGCTAGAG
    AAAGTACCCAAGGAGCTAAAGGGATCTGCAACCCTATAGGTAGAACAACATTATTAACTAACCAGTACCC
    CGGAGCTCTTGACTCTAGCTGCATATGTATCAAAAGATGGCCTAGTCCTCCATCACTGGAAAGAGAGGCC
    CATTGGACACGCAAACTTTATATGCCCCAGTACAGGGGAACACCAGGGCCAAAAAGTGGGAGTGGGTGGG
    TAGGGGAGTGGGGGTGGGTGGGTATGGGGGACTTTTGGTATAGCATTGGAAATGTAAATGAGCTAAATAC
    CTAATAAAAAATGGGAAAAAAAGATAATACAAATGTCAAAAGTTCTATAAATTAATACATAGTAAAATTA
    TAAGATTAGTGGAGTTTATAAAGTAAAATAAATATACTAACATATAGCTCCTTATTATAGGAAGTGATTA
    TAAAAAATTAAGAACTTTTTCATATCTTTCTATTTCTAATTATAAAAGAAATGGGGCAGATAGTTTCAAA
    GTATGAAAAGGCAAACATTAAATGAATATACTGTGGAGCTCTGTCTCTGTGGTATTCAGGAGAGATGTCA
    TCTTTATACTTTGCAAAGTTAACAAAAGCTAGGGCTTTGTGAGGCAATGGAATGGGCTCAGTGAGTAGAG
    TAACTTGTGACCTGCCAAGAGAACTCCCGTGGAGAAATGGTAGAGATGATTTCTGAAAATTGTTCTCTAT
    TCTCCACACTTACGCATCACTCGCATCCCAACTCTAATAATAAATTAAAAATGCAAATTTAAAAATTACT
    AGTCTGCATTCTCCTCCAGTTTTCTATACAGGCCGTGTGGGGATGTGACTGAAGATGCAGGCAAGGTGAG
    ATGATGATGATGATTCTCATTTCCAAACCTTGCACAGGAAAGGTAAAGGCAGGAAGATCAGAGTCCAGGG
    CCAGCCTCTCTAAAGAGGTAGCATATTAGAGCTATCCGGAGCATTTCAGCTCTGCACGGAAAGAGATGCA
    CTGAAACATGAAACACACTGAAATGATACTCATAAAAATCACCCTCCAAATCCTGCCTATCAACTGCATT
    CTACTCTATCCCGGGTCCTTAGGCAGTGCTTCCCACCAGTTCTTGCTCATCCTGAAAAAAAATCTCCCTG
    CAGAATACAAGCATGTTTAGGACATAGACTTTCAATCCATCATTTCTTCTATAAATATTAAATAACAAGT
    AGCATTTTGTGTGTCCTTGAATATCTATTAATGAGCAGAAAGGTCATTTATATTTCTTTTTATGCAAAAT
    GTCTATTCAAGTTCTTTTCCCACACTTGCACCCATCCTTCTCTATTCATATCCACAGTTTCCATCAGAGA
    AAAAAGAATTCCTACCTGTCCCCTCCAACCCCAGTTCACCAGTTTCATTTTCAATTTCTCCAGATTGTTG
    TTATTTTCACCCAAGGGGAGTTTTATACAATGAGTCAGGTTTATGTTTTTGAAGATGCCTGTTTTAGACG
    TTGAAACTGAGTAAGAGTTGGAATTTTCCGATGAGATGCTTTGGGGAATTCCTAAGTAACAAGTCACCAA
    CATGGAAGAGTTTTACTTCAGTCGGCTTGTTTTAGAGTGCTAGCTCACCCTCTTGTCGGCGTTGATAATT
    GAGCTGGTCCAACATCACCAGGACAGTTGAGCTTTGAGCTATGGACCTTTATCAGATGCCTCTAGCATTT
    TGTCATTAGTGACGCCATACTGTCTGGGCTACAGCTGTGTATATGTGCACACCAATGCAATGTTTATCTG
    TCTGTCTCTTACTCTGTCGAGTCAGTTATTGTGTATGAATTTGGGCATATACGTACCTGCTGTCTGTATG
    TGTGCCTATGAGTGTGTATATTTGGCATGCAGCTCCACATACAAGTGTGTGAGTGTGTACATGGAGGTCA
    GAGGTCAACATCTAGTGTTTCCTTTGATGGTTCTCCACCTTAAGTTTTGAGGAAGCATCTCTCTGAAACT
    GCAGCTCATTGTTTCATCAAGGTTGAGTAGTTAATGAGCTCTGAGGGTTCGCCTAGCTTTGACCTCAGCC
    CAGTGCTGGAGTTACCAATGTGAACTGCTGAATCCAGCTTTAAATAGGTGCTAGCTATCCAAAACGGATC
    CTCACATTTGTGGACCAAGATCCTGACCTACTGAGCCTTATTATTATTTTTTTAAACATGTGTAAAGACT
    ATTGAATATTTTTTAACTAAATTTATCCATCTGTGAACATTTGCCTATTATTTTTCTTTGGCTTCTAGGA
    TATGATGAATTACTTTTAGACAATAAGCTTTAATTTCTAAGATAAATCTTAATTGAACATAATATATTAT
    TCTTTCAGTGAACATCTAGGTTCTTTTTAATATACTAATGTGTTTCAAAATTATGTTCATAAAAGAATAA
    GCTAGGGTATCCACCACAATATTAATGTGAACCAACATTAACTCAAATAGCCTTATTTGGAAGGTGGTAT
    AGCATTTCATTGGCTTGAGAAATCCAGTTTATCATGGAAGGCAACACAATATAAAGTATGTGTTTTCAAA
    CTCACAGCTTTAAGGTAGAAATCAGAAGCAGTCAAATGCACTATCACCACGGACACTTCCTAAACATTTC
    TTTTAAAATCCCATGTAACAGAAAGTAGAAAATTTCAGCACCTGCGGTATCCCTCTAGGACTAATTCCTA
    ACATTGGCCCAGCTGATTCGTGTGGTACATGGCATTCAACAGGGGCATAGCTCATCAATAATGGCACAGC
    TCACGCCATGCCCAAGCCCTCCTAGAATCTTACCAAAATCAAGCATATGTTGTATCATCAAGAAAGCCAT
    TACAAAATTAAATTGACAGAAATCCTAGCTGTGAAAAAAGCAAGGAAAAATGATATAAGAACTTTTCAAT
    TGCTCGAAAATGAGGCAACACAGGTTTAACTAGTCTGTAGAACAGCGTAGAAGTCCCAAGAGACATCAGA
    AGTGCAATAAAGATATATCATGCTATCTTTAAGCATGGGAATATTTACATAAGTGAACTTCCAGGAAAGC
    TGGAGACTTTAAACTGAGAAAATAAGAGAAAACGGGAAAGTCAGTTCGGGGAAGGGCTGCAGAGTAAACA
    CTCCCAGTGCAGAGAGGCAAGCAAGTTGCACTGTAAGGAGTTACTCATAGACAAAGAAAGGGGAAGGAAG
    TTCTGCTAACTTCTGCCTCTGACTCATCACTGAGCAGCAGCACTTTAAGTTTCCCTGGGAAAAAGGAAAC
    AATTCAGACAGGTGCCTGCATCTGCCTGTTTGTATGGATGTGGTGACGTTCACTGGCACTTGGGTACCAG
    TTCTGTCAACTCCAGCTTCATTGTGAGACGGTGCCACCAAGAAACATGCCATATCTTTCCCATGAACAGC
    CTTCTTTGTTTCTTCCAACAAGTATTGCTGTTTAGATTAATTTCTCAGTCAGAGGGGCATGTTGCCTCTC
    AGAATATTCAGTTGATGGTGGTTCACTGTTACCAGTGGGTTTCCTCCATGCCCCTCTACTCCCTCTATCT
    CAGGGGAGGTTCCCCCACAAAGATTTTTGCTTGGGCCACAAAGGCTAAATTGTTTGGATTTCATAGTATC
    TTTTATATTCCTCGAGCTCTGCTATAGAATTATTTATGAGTACCCCCCCCCACCGCCAAGTCCCTGCCAC
    CAGGTCCAACCTCTTTATTCTCTGTATGGTTACCTGAGAAAACAGCTTATTTTAATATAAATTTAAGTAG
    TAGATTAATCAAAACAACTGTATTTCCTTACCTTTGCAAGTTTCCTAGTAACCATACAGAGAGACTAAGA
    TTTATTAATATTGCCTTGAGCCAGATACTTAGCAATCATTGCTCCACTCTTAACCTCCAAGCTCATCAGG
    TTTCCTCTCAGACATCAATCCCAGTTTATTTCCAGCCCTTTGTCCTTTTTGACTCAGCTCTCTTCTGATG
    TTTTTAATCAACCTCTCCCCAAGGTTCCCTCTGGTTCTTTCTCCACCCTTTCTAGAATCAGAAATCCCAC
    CTTGTTCTCTCTAAAGCTCTGCATTGGCTCGTTGGCTTTTATTGGCAAGACAGAAAATGAATGAGAAGCA
    ATGTTTAAACAAACTTGAGACAGGAGTTTCTAAGAATAAACATAGCAAAGTCATATCTAGATTGAAACAA
    GATATGAGAGCAGAGAAATCAATATTTGAATAAGACAGGGATAACCTTTATGCAGTGCACAAAAAAGTTA
    TGCCTACAGCTTCCAGAAAGATATTTATATTTGGCATCAATTGTTGCCATCGTCGGTATGGAAACAGAAG
    AGTTCCTGGAATGTCAGCATTGGTGAAGTAGCAATGTTAGGAAGATTCACAGAGATGATCAAACCAGTGT
    TCACATATTTTCTATAATATATGCATAAGTTCTGATGTCTATGATGGCTACTCTGGCTTGGCTGAATTGA
    GAAGATGCCCAGAAAACTGGCAATCCATACTTCAAGGGGTATCTATGGAGGGGAATTTGGGAAAAACTGC
    TTCCTGGGTGAATGAGCAGGGCAGACAGGGATCCTGAGAGGTGGGAGTGGCCATGAGCAAAATGGACCAC
    AAAAGTAGACGTGGGAAGCCAGCTTTCACTCACACTCTCAGTCCCTGGCCAAGTTCACTTATTTGTTGCT
    GTTGCCTGTGGACATAAGACTCCAGCCTCTTTTAATTATTGATCTTAGAATTGCACCAGAGGCTCTGTAG
    GGACTGCCAGGCCTCTGGTCTTAGACCAGGGCTGTCCCTTGGCTCTTCTATCATTCTGAGATTTCTAGCT
    TTTCCAGTTAATGCAGATTTAGCTCTCCACCCTGCAAGCAGTCACTGCAGGATTTCTCAGCCTCTGTGAT
    GTTCTGAGTCAGCTCAATAGGTCCTCATTTGTAATAGCATGTACTTTTGGTTCTGTTTCTCTGGAGAAGC
    CTGAGTAATATGCCCAAACTCCAAAAATTTTTTCTAACAAAACTGTTAGAAATATGCCAGAGTCATACAC
    AAAAGAAATGTATTCTATTGAGTATTTCCAATATCTACCCAAATTTAAAAAAAAATCAATGATGGCATTT
    AATATATTTTAAACTGCATTACTATGTGCAAGGTGTTAATATTTACTTCTGTAATTCCATACAGAAAAAT
    GCCATTATATGGTGTTAAGTACACAAAGCTACAAAATAAAATTTTCGTTCTTTTTTAATAGAGAGTATAG
    AAAACTAAGAATAGAGTGTTGAATTAGCTGTTAGTTACATAACTGGCAATATGTCTTCCACTTCACACTT
    CTCTCTTGGCTTCACATATACTATAGTGAACACACATTATTTTGATAATCAGAAGGATAATAAGAAATAT
    AATTGAAAACTTTAGCCAAATAGAGTTTAACATGGATATCAGGACAACCAGAGCCCATAGGCTGTGGTAC
    TCGCTGAAATACAAAAGGAGAAGACTTAATATGAAAACACATACAGGAGAGACCTCTGAAGTAGAAATTT
    CTGGTTCCTTCGGAAATTCAGTTATACCATATGATGTTGGCCTGTGGCAGACAGGTGAAAGGGTGTTTTG
    ACGAAGCAGACAAGTGAGGGGATGTTTTGCTGAAAGAGACACATGAGAAAGGGCACATGATGTTTAGGAA
    GAATATAAATATGACTCCACAGCCAGTGGGAGGAGGCTCTGGCATTGGTTCAATTTGCTCTGTCTTGCTG
    GTCTTCACTGATGGTGCTCTTGTATTGGTTGGCCTTGCATCATGTTGCTGATCTTTGCTTGTGGTGACTT
    CATAGAGAGTAACTCACCAAAGAACTTCTCATGATATTCTGCCAGCTTCTTGTTGCTTTAATGGACCTGG
    GGCATTCGCCAGAGCCTTGAGATTTCTTCTGGATTGAGCTGCTGTTTCTGATTCACGTTTGTGTTTGCTA
    TTAGACTGAACTGCTGAACTGCTGATACCCTGACAATAAAGACTGGAATTGTCCAAAGAACTATTTCTAA
    ACAGGTCTACAACCTCTGTTTCCTATTAACCTTCCTTTCCCACTACCTCTGGTGGGTGGTGAGTTAGAAC
    GGAGGTTGAAGCACTTAAGAACCCTTATTAGAGTAGGTTTTGAAAATTCTAAGCCTACAGAACCTGTATT
    TACATACAACATAAACAATTTTTTTTCATTGTACAAATGTGTATTTTTACGATGTAAGTATTAAAGGGCA
    TGAAGAATGCAACAGGTGAGATGGGCCAACAAGACTGTCACTGCTGCCCAGGGTTCTTGACTGTAGGGCC
    TATGAACGTTCTCAATTGAGTGTCCAAAAGGAAAGCTGCTTTGATGGCAAACAATCCTATTCAAAGTGTG
    CTTCCTTGGGTCCTTATGGTAGTAAAGAGCAAGGGTCATGAAGTGACAAATTTTACTTAATTGCCTGTGT
    GTTAGATGCAAATTAAAAGAGATTCCTTAAGTTTCTAATTCAACTCAGCCTAAATTTCCAGCCTTCCCTC
    ACATCCATTGGAAAAGCTATCACTGGTCATCCTCCTATAGCCAGTTGAATGACCTAGTTTCTGGCTTTTG
    AACAAAGGAAGCCAGAACGAAATTATTTAGGGAATCCAGAGAGTTGAAAAAATTCATAAAGCATGTGACA
    GTTTTCTTATGTCTTCAGGAGCCTTATTTGTTACTGGTTACTGCTGGGTAGGGCATTTCCTGCTTAAGTT
    TCTTACATCCTTCAAGTGCAGTTTGAATCAAAGAAAACTCTGGCACAATAGTTTATTAGTCCTATAGTTT
    ATCACGTGGAAATTTTAAAAATATGATGGTTATTTATTTTTTATCACTATCTCACAGTGGCAGAGTAAAT
    TGGAGCTACAGTGTTTTTGCTAAAATTGTCCCAACTTCTATAACTTCAACATCCAGGATACTGCCTGTCT
    CTTCCTTGAGCCCCATGGCAACAATTCCTAAATGTGTGTGTCTAAAACAATAAACACGAATTGATTTTTG
    AGTTCAGGGAATTTGCCACAAAACTAGCTTCCCTTCGAGAAAGCCTGGGAGATCACTGAGTAAATGTCTG
    CTTAGTTGATCAGTACCCAGGACATGTTCTTTGCACTTTTTCTTTCAATTTTTATTATCTTTATAATTTT
    CCAACAACAAAAAAATGACTGAAAAAGACAGGAATTAACTTTCTGTTTTGGATTCTTGTAAAGTTATTCT
    TCTAATAAGCCTGGTCACTTCTATCACCTCCCTTACCTTGTCTACTGTCAATCTTGGTCTGACCACACAC
    GACAGAATTTTGTCTAGAGGTCACACAGAAATTACAACAGAGAATAACAGTGAAAGATATGCATTTACCA
    ATAAGATGCCAGAGCTTAGTTTTCTGGGTGTTTGTTTGCTTGGTTGATTGGTTTGGTTTTGCTTGGCTTT
    GCTTTGCTTTGCTTCTTTTTATAGCTCCCTACACTCAATGTATCCAGACAGTGTTCTCATATAGTTATCA
    AATGTTGAAATTATTGATCAAACAATCAGACCTGTGCTATTTTCTAATCTGTTTGCATTAAAAAAAATTC
    ACTGAAGAAAGGGATCTTAGAATTACTTTTGAGTGAAGCATGAAGTATCCCATTCTTTCATATTACCCAG
    GAACAGAGCAGAAAGTGAGGGACAGAATTTATTCAACATAGGTGCCAGAACCATCTGGTTTGGAAATGAA
    ACTCAGCTCTGGACACTCATATTCATCAATGACTAAGGTTCCTATCAACCCACGTGATAGAGGATAACTA
    GTCTGGACAGAGAACTTCTAAATAAGATGACCTAAAATATTCTGATCTGACTCCAGGTAAAAGGAAACAA
    CCACCTATGTTATGTGCCATACTCTCTGACCTCTCAAATGCTTGTAAAGGCTGGCATTTCCTTCTCCAAT
    AACATGCTCGTTTTCTTAAACCTTGAGTGTAAACAACAGCACCTTGCTTTTCATGGCCTATTTATTGTTT
    GTCACATTATATTGCATTATTATTACATTGTTGCATGATACAATTCTTAAAGTTTGCTTACTTGGCCTCC
    TGTAGGCTGGTTATCTGCTGACATGGGTTTCTGTGTTCTTAAGGACCTCACCAGCAAGGACCATGAAGAG
    GCAGTGCTTTGAAGAGCCAGAGGGCCATGTGTCAAGGTGGATTCTGTAGGAGTAAAAGGCAAGGCTTAAA
    GAGATAAAATTCATACTGACTCTTTTCCTCAAAGTTATGGTTAAATATGGACCTGTCATTCTGATAAAGA
    CTTGTGTGGAGAAAACTTGAAAAACTAGATCTGAATTGTTTTTTTTTTTTTAAAACATTCCTAAAGTCGT
    CAGTTCCCCACAATATAATTGACACCCATGTTGACTGTAACTCTCATAATAAATCAAAGCAAATAGGACA
    TCAAAGATAACTGTGGGCTTCATTACAATTACAAAATCTAAGCAAGATAATGGAAGTTACTTGTAGACAT
    CTGTTCTAAGATTTTCCACTCCTGTACTTAGAAATGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
    TGTGTGTGTGTGTGTGTGTGTGTGTTTTATCATTTACAAAGTATATCTGCTTTTTAAGGGGTCTAGTTAC
    ACTGTTAGAGAAGCTTAAAATAGCCTACCTCCTATTTTGCTTTCCAGAAGTACACACTTTATGCTTTTCA
    AGAGTAAAATAATATATTCTATCTTTTAAAATAATTAAGAAACATGCACATTACAGAATGATTAATGATA
    TGAAGGATATCATATTTATAGCTTTAATATTATGTTAGATAAGTTCTTCTGATCGTTATTGGGATATTAG
    GAGTTTGAATAAAAAAGAAGCAGCTGCATTGTGTTGCCTAAACTTCCCAAGTGAGTCCCAAGTGAGGGTT
    TACATATTAGCTCTAAGACTAACTCACAGAGAGGAGAACTCTAACTTAGCATTTGATATCAGCAGCCCAC
    CCCAAGACTTCATCTCTAGTCCACATAAGGAAGAGTGAATGCCAGGGAGGAGAGGAAAAGGCAAAAACTG
    GAGGTACAATTCAACAACATCCCTCCTAGAGGATTAGCGCTCAACAGGATGCTAGCTTCTCCTTGTCCTT
    CTCTTTCCCATCCTGTCATCCTGTCATCTTCTTCTTTCCTTTCTCCTCTACGATGTGTAAGAACATGGCA
    AAATGATAGCCATCTCTGGAGCAGGAAAAATCATCTCACCAGAACACAAGTCAGCTGGATTCTTATTCCT
    AAAATCCACTGCCTTCAGAATTGTAAAAAACAAACTCTCCCTGTTTAAACCACTCAGTCTCTAGAACTCT
    GTTACGATACCCACAGCTGACTCATACAAATTATAACACATCCCCACCAGCCAGGAAACACCAGCCAGGA
    AAATTGATTTTCCCTTAAATATGCCCGTAGAAAGGAATAAAATAACAGAAAAAAATTATTGAAGGCTTTG
    CTTCAATAAAAATAAATAAAAATTTCCACTCTGGAAATCAGTCTGGCGGTTCCTCAGAAAATTGGACATG
    GTACTACCAGAGGATCCCGCAATACCTCTCCTGGGCATATATCCAGAAGATGTTCCAACTAGTAAGAAAG
    AAACATCCTCCACTATGTTCATAGCAGCCTTATTTATAATAGCCAGAAGCTGGAAAGAACCCAGATGCCC
    CTCAACAGAGGAATGGATACAGAAAATGTGGTACATTTACACAATGGAATACTACTCAGCTATTAAAAAA
    TGAATTTATGAAATTCCTAGGCAAATGGATGGACCTGGAGGGTATCATCCTGAGTGAAGTAACCCAATCA
    CAAAGGAACTCGCACAATATGTACTCACTGATAAGTGGATATTAGCCCAGAAACTTAGAATACCCAAGAT
    ATAAGATACAACTTGCCAAACGCATGAAATTCAAGAAGAACTAAGACCAAAGTGTGGACACTCTACCCTT
    TCTTAGAAATGGGAACAAAACACCCATAGAAGGAGTTACGGAGACAAAATTTGGAGCTGTGACGAAAGGA
    TGGACCATCTAGTGATTGCCATATGCAGGGATCCATCCCATAATCAGCTTCCAAATGCTGACACCATTGC
    ATACACTAGCAAGATTTTGCTGAAAGGACCCAGATATAGCTCTCTATTGTGAGACTATGCCGGGGCCTAG
    CAAACACAGAAGTGGATGATCACAGTCAGCTATTGGATGGGTCACACGGCCCCTTATGGAGGAGCTAGAG
    AAATTATCCAAGGAGCTAAAGGGAACTGCAACCCTATAGGTGGAACAACAATATGAACTAACCAGTACCC
    AGGAGCTCTTGTCTTTAGCTGCATATGTATCAAAAGATGGCCTAGTCGGTCATCACTGCAAAGAGAGGCC
    CATTGGACTTGCAAACTTTATATGCCCCAGTACAGAGGAATGCCAGGGCCAAAAAGGGGGAGTGGGTGGG
    TAGGGGATTGGGGGGGTGGGTATGGGGGACCTTTGGGATAGCATTGAAAATGTAAACGAGGAAAATACCT
    AATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAGAAATATTCTCTAATAAGAAAA
    AAAAATTATTGAAGGCTTTGCTTCTCAACAAAATCCTGTGTGTTCTCACTAATATCCTAGAGTGGCTACT
    ATTGCTGTGATAGATTCCATATGCAAGAGCAACTTGGATTGGAAAGGGTTTAATTCAGCCCTCAGTCTAT
    AATACATTATGAAGAAAATTTAGGGCAGGAACTAAAGAGAGGCTATGAAGGAAAACTGTTTACTGCCCTG
    TTCTCTTTATCAGCTGACTTTTTTATATCACTAAGACTAGCTGCCCAGGGATGGCACCACCCACAGAGGG
    TCATCTTCTTGATTAATGATTGATATGGGAGGTCTCAGCCCCTGTTTATTATTTTTTTCTCAACAGGGGT
    TCTCTCTTCCCAGTTGATCCTGGCTTCTGTCGAGTTGACAAAAAAAAAAAAAAGTCTAACAAGACAAAGG
    AAACTAACATACTCAGATCTGATGAGCCATAAGATGAAGAAACGGAATTGAAAGTGAGAAATTCAGCCTG
    CCACCCTCTATAGGCCAGTGCAGAAGCATTGTTAAAGAGACCTCACACCTATTCTGGAGGAGCAGCCCTA
    ACCAAGGCCCCCCCATCCAAAAGTCTTCTTCAATCCTTAGCTGGTCCAGGAGAAATTCAGCCTCTTAGCC
    CTCTACCATCTCCTCATGTCCAGCTTGAGGCAGTTAGAATCAGAAGGAAGCTAGAAAGCATGCTACATGG
    ACTGTAATGTTCCTGCTTTTAATTAGGAAATATGGGCATAAGACTCTCAGTATAAGACTAGATAATTAAT
    ATAGTCACAAAGTTGCCCCTATGTAAAAATTAGGGAAAGTAATAAAATTTAAAATGGGAGGGGGGAATAG
    ACAACGTGCCCCTCCCAAATAAAAGGCATAGGAAACACCTTGTAGCTTTTGAAGTTTAAAATTGCAATTT
    GAATGTATCTATGGAGTCAAGCATTAAGTTTGAGAAAATAACAGCAAATGTTAAACGTATTAGACAGGGG
    AGGTTTAGTTGCTTTCATTAATCAAGCAACTATGAAATATATCTATGTTATCTTGTATAAAGGTATTTAG
    GCTAAGTGGTGCTTGAGAAGACTATGACCATGTCACTTAGCACTCTGAAAACACCATGTCTCATTCAAAG
    GAGATGAGAAATATGTTTAACTGAATTACCACCCCTCTTAATGCAGTAACCAAGTTAAGACCCTTTGCTT
    TGTTTTTGTTAGGAAAAGAAAAACAAAAAACAACAACAACAAAAACTCTAGAAATGGTGAACAACCATTA
    CTTTCTATGAATGTATTTTCTGTGTCTGGTTTTCCTGTTCCTAACTTAGTGATCCACACCTTAAAAACAT
    GCAGAGCTGTGCTGTCAAGCTGTGCACAGTAGCCACAAACCCTACGTGACCATCTGAGGGTAAATGAAGT
    AAAAGAGAACAAACCAGAAATCCACTTCCTCACAGGAGCCAACCACAACTCCAAAGCTGAAAAGCTGAGG
    CACATGCAATTTTGAAACATGACTTAGCCACCAGCCAGCAATACCACAGCATGCGTGAGCTAACTCAAGA
    TACTTTCACCCGTGGCATTGTGAAAGCAGTAATATCTACCTCACAGGATGATTGTGCAATTCAGCATCTC
    AGGCATGTGAGTTGTGTAAAATGGGCCATGGCACACAGAGAAGACTCAAAACTATTGTTGTATTTGTATT
    ATAATTTTATGTTCATATTTGATTCTTTTACTGCTACTGGTGATTGGGTGACTGAGACTTAAACTGTTTA
    TCAGATCTAAGAACATGAGCCAATACATTTGTACCTGAGATTATGATAGTCTCCTTGAGATGACACACCT
    CTGGACAGGGTCTTAAAATTTCGAAGAAAGTGGTCAAAGATCATGAGGAGTTGGACAAAGCCACAAAGCA
    GAACAGGAGGGTGAGGTTTTCAAAGGTTGAGAGCTTGAGATAAATTTCAAATAACATAGGTACTGCAGAG
    GTAGAAGAATGAGAGTGGAGAAGATGTTAGAGGGATCAGGCCACGATGTTGGAAAGTGAAGCAAGAGGCA
    GGGAAGAAGCTCTGTAGTCTTTGCTCCTCAAAAAATATATTTCCTTTCCATCCATAGGCCAATCCATATC
    TTGTTTATTTGTAGGTTACAGCACCAAAATAGTCTAGCTAGGCTTGACTTTAATAGTGGTCACTTTTGTG
    TTCTTTCATCCCTTCTCCATGTTATCTTTATATGAAAGCATTGTGCATATATGCTACTTTTAATTGAAGA
    AATAGATATGGAAAGCTGTTTCCTGAACTTTGGAGATTGGCTGTTAAAAATAAACTACCTCCCTCCAGTC
    CAGAGATATGTCATAAACACAAAAGATCCCCAGGTATGCACTTAAAATAAAGGATCTGCTTTTGATTCCT
    TGTGGCATTTAGATAAAAAGATCCATATAAAATGTCAGTTACAATTCATTATAAAAATCAGCAAATCATC
    ACAAAACAATTTTCCAGTGCTGCCACTAGAGATAATTGTTTAACTATTAAAGTGTATGCTATGATTTATA
    GCAGTCACTCTGATTCCAATCACAGCGTGATCACTCAGCACTGCCACAGAAGACACACCCCATCTGTTTG
    TCTCGGGAAATGTTGCATGACGACTGTAATGACGCGTACCTGCTATCACCTGCTGATAACATCCCGTCAG
    AAGGAACAACAGCCTAATGACACAGCTCAGCTCCTTCTGGCAAATGGCCAGATTCAAATATTTCTACTGC
    CTCCCGCAGAAGTGAGCAGCATGATTACCACAGTCCTCGGTAATAACGCCATCAAATAAAGCAATCTAGA
    ACAAAGTGGTTATTTTGTAGAATACTAGTATACTTTTGACCACAAAAATCCCTTGGGGTCTATTCTGTCT
    ACAGACAGTCTCACCAGCAGTGACTGCCATTCTCCTTTGCTTTAATTATTCTTGCTGATGAGACCTCAGA
    CATCACTTATTTTGAATACTTGGCCTTTTGTGCAACATAGAGAGTGGTTTACACTGTATATGTCAATCAA
    CAGAGTAGTAACAGATTTCTGGAGTGATCCCAAGCATTATTTGTCTACAGCATTGCTTGAAGGGCCCTTC
    CTGACAAGGGAACAAAGCTTCTAGCAGCAATCCAACAGCTCGCTGAAATGCTAAGATCTTGATTAACCAA
    GTTTATTTGTCAGTTGGCATAACAAACGTCAGATGCCTGGGCAAAGAAAGTGGCTACTGCTTTCCCTCTC
    CCTGGCCTAGTCAGCAGACAGTATCCTCACTCTCAGTTATCATTTTTTCTTCTATAGGGGTTATGTGCAG
    TTATTTCAGGTAGAGCCAGTATATCCCTGGCCTCCAAGACAAAGGACTTAATTTAAAAATATATCTTCCT
    TCCATTGACCTAATCAACTACTACTTGTTAAAATAGTTATCAGAAGGCCAACTATTACTCTGGTAAAGTA
    GTATTCATCAAGCTACTACTAAGGATGGTCATTTTTACCTTGTTAATTAAAATTTTTTTCTCTGATTTAT
    ATTACTTCAATCATTCTCTTTAGATTCAGTATCAAGAAAGTGGGTTTCCTAAGCACCAGCAAATGCTGTG
    CCATTCCTTGTGGGATGAAGCTTGCAATACTCCAAGAATTGACTTGTTGAGGGAGGAACTGAAGGATCTG
    GCAGACAGACATCAAAAACTTGCTTCTTACATCAGGACAATCTCTCCACATGGAAGGCTGCCATGCTACA
    TGACTAAATTACAGAAAAGTCGATTCTTTAACACACACACACATGTTGAGAACAAAGACACCTTAAAAGG
    CTTTGCTATAACTTAGGACTTAACATATCCTAAAATGTTAAGTCATATAATTCAGGTGTGGTCCCCTGTG
    CAAATGGTGTTTGGAGGCAGAGTTGTGGGGAAGTACTTGCATCTTGGAAGTCCTGGCTTCAGAAATAGAT
    TATTCTATTGGTATATCAATATTTGAATAGGTTGTACAGAGGAGGTAGAGCTGGGTCCTAGTTAAGGGAA
    GTAAGTGACTGAGGAAACACCTTGGAAAGATAAATTGACCCCTTCCTGTTATATTATGCTATCTGCCCCC
    TAGCACCTTGAATTGTACCTTTGCTCTCTGTCTTATTTCTGCCTCACTACAGGCTGAGAGACACTGCAGC
    CATCCAATCATGGACTTTAAACTAAACTGTGAATCAAAGAAAATTATTCCTACTTCCCCCTCTGCATTGT
    TTGTCATATTGACACCAACAATAACAAGTACAACACAGTTTTATAGAATTCATATGCCATGTAAAAGCAT
    AACATAAATCATCCCCATGTAAGCACGGGCCATAGCCTTCTAGGCAAGTAGCATCCAGTATACCCCTAAC
    ATTTGCTTACAAGAATTTGTTTCTAAAAAGGCAGAATCTCTGTCTGTGGATTGTTCTCTGAACAATGATA
    GAGGATTCACCATCTTCTATTATATTCTCATGAAGGCTACCATGTTCACAATTAAGTTACTAAAATAATG
    GTAGCTTTATCACATTGAAGCTGAAGAATTTTTTATAAAATCTGGATCCTCATCAACATTGTCAGAACTA
    TGAAGGAGATGGGAAGCCAAGAAACATGAATATACATTTTAGTTCTGTCTTCAAGTAGCTTCATGGCCTT
    AGACAACTTATCTTTGAAATACAGAAATTATATTGGAGGTGACCCTAATTCAGTGTTGAAGTACCATACA
    TAAAGTCATAGTTGACCACCACAGCATATATTGCCAAGTCTTTTCTTTCTTCAGAATATCAGATGTAGCA
    GCCCAAGAATTTCATCTAATCATCTTCTTTTTTTTTTTCAGAAAAGAAAACACACAGAAATTAAGTGCAT
    TGTATAGATTTACACAGTGAGATAAAGTTTGGGTTCAACAATGTGTTTTGTCCCCCCTGCCCCGGGGCCT
    TTGCTGTTCCCCAGTTGGTTCTTGATCTGTCAGTAAAGAAGCCTCGAGACAATTGCTGGGGGCTTGGGGG
    TCGGGGGAGGAGGAATAGACAGGACGTCCAGGTCCCTAGAGGAAAAGAGACAGGTACAACTCATCATGCT
    TTGGAGAGAGAAAAGCTAAACAGCCATGTGAGGTCTCAGGAGGAATGGCCACTCACACAGGTGGGTGGTC
    AGGGATGTTTACTAGGGACTAGATGTATCTGAGCCACTAAAGTTTAGGGCAGGTGGGAGGTGCAGAGATA
    AGACTATTAATAAGGGCACACTTTTCCAGGTGGAAGATAATAGTACCCAGCAATTGTGCCAAAAGGCAAG
    TTGAAAATGAGCAGCGTATATGTATTTTATCCATGGATTCAAGGAAAACTGGGTGGGGTCTGGTAACACA
    GTCCATTCCCAGACCTTAGGCAAGGTAGGGAAAGCTACATGCAACACCCTCTCCCCAACACACATTAGTT
    ATCTACTAGAGTTTACTTAGCTACCAAGAATATGTGAGCATTATGCTAGCTGCCCACACAAACTTTAGTT
    CCGAGATGAAGGCAACTTGTTCCAATTAAGTAGGATTGTGATGGTTAGTCTTGCAACTCTACATGAATTG
    ACTATATATAAAGGTGTACTTATTAGTAGATTTTTGAATTGCTGTTTTACAGCTTATAATTAGCCATCCT
    ACAGCATCAATACTATGATTCAAAATGCTATCACACTGCTATGAATTCAATCTACTGTTTGCAAATAGAG
    AGCTTAAGGGTCAGAACCAACCAGTGTTCCAGAGTAATTCTCCTTATTCTAACCCACAATAGCTCAGTTA
    TCTAACTTCCTCCTTGACACTTTTGCTAACAGCACTCTCCTTTTTGCACTATCTGGCAGACCATAAGTAT
    CCTGTCTAAACATGCTCTTATCGTTGTCTTCAGCACTTCCTTTAAGGTTGAAACAATGTTCTATCCTCAT
    TAGCTTCCAAGAATATTTCTTCACATTTCTGTATTAAATTCAATATTTACTATACCTTTTTAAGCATACA
    GTTCTCCAATTTTGAAGGGTCTCATTTATCTTTACTCCCTTTGGAGCTTGACATTAGCTTTTTTTTTTTT
    TCCCTCATTACTAAGAATGTACTGACAGGTTTTTTTGTTGTTGTTGTTGTTGTTCTGTGTTATCTATTCA
    GTCTTCAAACCACAATTTGTCATGGAGAATTGTTGTGTCTCTGCTGGGAGATGTTGTATGATCTGTACTA
    ATGTATTATGCTAGCTAGTGATAATTTACTGTTGAAAAATATCATAAACTTACTCTCTAGTAAACTTGCT
    GAACTCTCTAGTAAGTTTTAGTAATTTCTTGTTTTACATAGCTTTCATATATAAATTATGGAAGTTTACA
    TTCTCCTAGTCACATTTTTATAAATGTTGATTATTACCTGTGAGAACTACTAGCAAATAATAGTCTTCGT
    ATGAAGAATGCAGAATCTAAAGTGAGACCTGAGTTCAAACCCATTCTATCACCTTCTAAATGACAGCACT
    GAACAGACTATTTGAGTCATATGTGTCAGGGATAAAAACCAGACACACAGGAGAAGAAGATGTTGGATGA
    ACCAGTACATTCCTAACAAGGGCAGCATGTCCTGATGCCTGTCTGTGAAGGAAAACAATGCATTTAAAGT
    CAGTCATTCTACACCAAGGGTACTCAACTCTAGATTGACAGCTACTAACTGCCCCATTAGATAATACATC
    TCTGGAGAGTTTCATGATTACTTATGTCTGTCTCTCACATAACTTGGCAGAGAACCATACACAGAGAGAG
    TGATCAGTAAATAGGCACTATTGTTCAGCAGAACTGCCCTGATGTTAGCCTTCCAGAACTAAAATCCACA
    GCTTGGAGGACCTAGTTGGTAAATAACAGTGGCTGGGGTTAAGAAAGGCCAAGGTTAGTAAGATAAGTAT
    ATTCATGCCAAGTCAAACAAGACCTCAGCAAAATTATGTCTTATGTTTCTTCACAAGCAACCACTAGGGC
    ATTGCTTTACAAGAGATAACATCTAAAGCTATAAACTATAGATTTGTAAATCATCATTGGACTCACACTG
    TGACATTATAATTCTAAGATGATATATTTAATCTTTAATATTCTTGTGAGAAGAGGCAGTAGATAAATTG
    CATGTCAACAGCAAGCCTAGTTTTGTAAAGTTCCCTTATCCTCTTCTGTTGAATTGTTTCTAAAATACTT
    GGCAATGAGAATTAAGAAAACCAAAAGAGCCCTGTTCGAATGAATTTAGAGATTAGGGTTTGCCGTATTT
    CAATTTCCTTTCATTTATGAAAGCATTTTCTTTGTAATTGCAAATCTGCCCACTCTATAATAAAATGTGG
    CATAAAATATTAGACAGCCCTGGGTTGGTTTTATTATGTATTCTATATTCTAAAACATTCACATTTCTAA
    TAATGAATCCATGTATTCTGCCTGCATCCTGGGTCAAAGTATGCTCCTAGGGGATTTTAGTTCACTTCTT
    CATCCTAAAAATAAGGAATACACTAAGATTTCTCAAAAGCCATATGATCTCACCAGGGATAATTCATACT
    GGTCAAAACTAAATACACTTCCTCAGACACTTAGTTTTCTTTACACTCAACAAGGAGTGGCTTAGAGGCA
    GCACAAATAACATTTGTAAAGCTCCATAATCTTACAGGCAAGGTGGCAGGCATTCAAGGATTCACTGCTT
    CCCTCATGTCAGCCCTTCTGACTCCATTGCTGCAGCTCTGAGTATTCTCCATGCCTGAGAAGCTTCATAA
    ATACGTTTCCATTAAACTGAGCATTACTGAAGCTCTATTCTATATGTATAGTTCCGAATCAAACAATTCA
    ACAAATAAATAATATACACTAATAAATCAATTATCCTTGAAATTGTTCTTCTTTCTTTTCCCAATTGTTT
    TTATTCTTGGGATGCATGTGTCTAGTTGGTTGGCTGGTTGGTTACTTGGTTGGGTGGTTGGTTGGGTGGT
    TGACTGGATGGATGGCTTTTGGACACAAGGTCTTACCAAGGTAGCCTAAGCTGACCTTGAACTCAGCATC
    CTTCTTCCTCCACCTACCAGTACTGAAATTGCGTGGTGAGTTCATAAAGGTAGAATGCTGACAAATTTCT
    AGGAGCAAGACAGGAGACCGGTATAAAGTTTGATTTGGCGTAGAAACATTTATACAAGAAGTGACTTTTT
    AAAGACATACATGTGAAGATAGGCACTGGGTAGACAGAAAAGTGTTTCAGAAGACAGCAAGCAGTGCAAG
    TTTGACATGCAAGCAAAAATATGTGACGCTTGACCTTTTTACTTGAAGATCTCAGAAAAGAGCAAGGCTT
    TCCCAAAGTCTTCACTGAGCATTGCTTCTCCCAGGGTAACCAGCTCTGTGACAATCCATTGTGTATATGA
    CCTGACTCCTGAATAATAGTCTCAGAATTGGCCATAACTGATAATTTTTTACTTGCTAATTTACAGGCTA
    TGCTCTTAGTCATTTGATCAATAGGCTAGTACTCCAAATGACTAATATTTTCAATTGTGTAATCCACTTT
    TATTACAGTCTTGAGATTCCAAAATTAGTATCTCCTTTTCTTATTTTAGGTGTAACCAAGATCTATCTAT
    CCTTGGCACCTTTAATTTTCCTATCTCTCAGGATTAATTCCCAACTCTTGTCTCATGCCTCCTGGTGACT
    CAAATCTTGTCAAGAGTTAGAATACAGACCATATAGTCTAGGAATTCAACATTACTGAACTGGCAACTGA
    AGCAAACAATTTAATGGATAACTAAGATGTTAATAAGTAGATTCTCTTTGAAAATACCTCTTTTTTTCTT
    TCTCTCACTAGTTTATATTGTTCAGTTGGTTGATTGGTTGTTTGGATGGGTGGTTGTTAGATGGTTTTCA
    AAAACAAAAACTTATGCTGTAGCCCAGACTGGCCTTGACCTCAACATCTTCTTGTCTCTGCCTGTTGGGG
    ACTGGAATTGCCAGGTATGGGCAACCATGCCAAGTCTTTACTATTTTTTTAGCCTGTTGGTATTCCTTAG
    TAGCAATGTATCATTAATTCGTTCATTCATTCATTGAATTTCTAATAGAAACTACCATATGCCAGAACCA
    ACAAATGACAGAGCTGTGTCAATGAGGAAAATAATAAAAATATATGTTCCACTATAGTTTACATTGTAAC
    AGAAGAAAAGAATTGTTCACAAGTTATTAAATCAAGCATATACACATCCAGAGTTCCTTTTTATGAACAT
    AAGAAAAGCTTTGAAAACTGAAACTTTCTCTGTATCAGTTAATTCAACATGAATTGAGCAGATATATGTC
    TCTATAAACTGCATTTAGTTCATTTTTTAATTGGTATCTGAATGTCTTATTGCTGCCATTTGCACCATCA
    GATATTGTTTAACTTTTAAAACCTGGAAGCCTGTATTTCTAATCATATTTGATCCTCTAAGTTTCAGACC
    AAGTATTACAAAGTTGCATTGTATTAGGTAACAGCAGAGATGTAGAGAAGTTGTGAGGTAATGGCTGTTG
    TTGGTCATGCAATTTGAAGACTGGTGATAACAGAAAACTTCAATGTAAATGGGGTACTCAAGTCAAGCCC
    CAAAGGAGGAGGTAAAGGAGAATGGACATATCTCAGATAAGAGAAGTCTAGGCAAAAATAAAATAACAGA
    AACTTCAAAGGTTCTTGGGCAAGAATGTAGAATGATGTTTAATAAGCAGAAACACTGTATTAGCAAATCA
    GTGAACCAAGAAAAGGGAATGAAGAGATACTAAGCATACCAGTCATTATAACTGAATTTGGATTTTACTT
    AGAGGAGAAACCAGAGGAAGATTCTGAAAAGAACAGTAGTGTGTCCTAAGTTTTTGTTTGTTTGTTTGAT
    TGTTTGTTTGTTTGTTTGTTTTTTACAATACCACCCTTTGTAAAATATAACTTCCAGAAATAGCTAACAC
    AAAGCAGTGGCTTCCAGCCTGTGACTATCTATGCCAGGCTACTTGTCAAAAAATGATGTCTATGTCCTCT
    GATCTTGAGTGGAGGATGGCCTTATGAGTTCTTTTTCCCAGAAGAAGGAAACAAGTATGGCAGTCAGAAC
    TGAATGTTTTGTCCTTAAGTGTTGGCAGGTTCTACTTCCTGTCTTACAGGAGTTCAAGATTATATAAAGT
    GGACATGGGCTCCAACCCTTAACCCAGAAGATTTCTGCAATTGATTCCCACTAACAAAAAGACCATTAGC
    AGGTATATTTACCACACATAACAGGAGGCATCATGCCAAGCAGTAGATGGCCAACACAAAATGAACTCAG
    TGGAATTTTTGTAGACTCATATTACATTCTTTGGGCATTTTTTCATCTTAGTTGGCTTTTGTTTGTATAC
    TATGGTTTCTGATGTTGTGTTTTTATGGGTCCTGTGTGTGTCTGTGTTTCTTGTGGTTTTATTTTTAAGA
    TTTATGGTGTTTTTGTTGGTTTGCCTGTCTGTTTGCTTTGTACGGCAAGAAATAATAGGCTTGGTGTTGG
    TTGGGAATGGAGGTGGGGCGGATCTGGGAGGATTCAGGGGAGGGGAAACCAGGATATATCCTCAAACCCC
    TGGGTGTAAGGAAGTTACACCCAACCAGAGGGAAAGTCTTGTGAAAGACAACTAAGTCACACAAGCAACA
    GGATAAGAAACGGATATAAAGTGCCATAATCCTGTGTCCATCATTGTCTTGGGCCATAATGAACACACCC
    CTGCTCAAAGGGTGCTGGCCTTGATAGATAGTACTGAAGGAACAAAATGGACTTTTGAAAGTGACAATTG
    TCTGCATTTAGGTGCTAAGATTAAGTATAAGACCAGAAAAAGAAGAGAATTTCAAGCTGACTGCAATGGA
    CCATACTTGTAAATACAGCACAGGAGAGGCAGAAGAGGTGAATGACAAATCAAGACCACACTATATAGCA
    AGTTCCAAGACAATGTGGCACTTTATCCCAAAAACAAAATACTTGACTACATAGAAATTAAACTTGTCCT
    TGCTTGTCTAGGCATCAACAAATTTTCAAAGGAACACATAGTCTTGGGGCAAAGATCAAATCCAGGGTTC
    ATTAGAACAACCTACTCTCAGGTTATAGATTATATGACTTGTGTATCTGAATCTCTCATTAAGACCTGAG
    AAAGTTTTAAGGTCATGTCTTGCAGATCTGACCAAGCAGTCTAAAGGTTACATAGTACCATATTGGTTCT
    TCTATAGTGTTCAGATTCACAGACAAAGAGGAACCAGTCTTGGGGGTGATGTGAATATTGTCTCTTAATG
    GAATGAATCTCAATAAAACTCAAAGGAAACCTCAACATTTAAAAGATATTTAAACATAAAAGAAAAACAA
    ATAAGCAAATAAAAAGAATAACAGCTATATAAAAGAACGTCTCTAACTCTAACAGCAGGAAGTATACAAC
    TTAATTGAAAATGTGTTGCTATGGAAAAAAAGAGGAAGGGTCCAAGAAGGAGCCAAATATCCAAAAGTGA
    AGATAAAGGACAAGAAACCTATCCTCTGGTGTTCACCACTCAAGTCAGTAGTATTAACCTGGGAGAGCAG
    AACCTAAAAATGAGGCAGAAGAAAAGTCCTGTACAATGGCCAACTTTATTCAGAATATCAGAACAAATTT
    ATGTTCTAAGAGTTTAAAAAGAGTCACCTGAGTAAAGTGTCCAATTACAAGAACAAGCCACAGGTGGTAA
    AAATATCTTTTTCCATAGAGGCATATGAATGACAACAGCTGAAGTAAACTGACTCCTATCTGCTGCACCT
    TAGAGGAGAATGAAGAAACATTCTGAGAATTCTGTGTTGTGAAGAAACTGAGGTCACAGGACACATGTCT
    TGTTTCCTTGGAGTTTTCTCATATGTGCTCAGAATTCCCCTCACGGAAGTCCATTCTTGGACTGTGTTCT
    GATTCTCTGGGAATAAGCCTGAACTTTTATAGGCCTACTTTCTGCTTTGAGGGTAAAGAGAACTAAGAAA
    ACAGGCCTGACTGAGTTTTGAACTGCTAGGAACCAGCGGCTATCACCGCCCTTTTTTCTCAGTTTTCAAT
    GGCACTGTCTATTGTAATTCTCCTGTTCATATCATCTCACTCTACACTGCAGGCAAGAGAAAGTCGCTAT
    ATTTTTAGGCCAGAGAAACAAACAGCACCAGAAAAAGTGTATCCATAATTTAAAGATGTAATTTAAAATC
    TTCAAGTCTGAGCTACAGACAGCAATATAATAAGCCATGGGGTGAGAAGTGGCAAAGTGTCAAAGTCCAC
    CATTGCTATCATTGAGATATTGAACATTATGGCTAAGTCAGTGTCGGCATGAGAAAAACAAATTAAATAT
    AACTCACACTGACTTACACATTGGTTATCTATACAACTTAAAAAATGGAATTGTCACCAAGTAACATAGG
    AAGGACTATGGGGACAGGTGGATTATGGAGTAAAATTGAGAATCCAACATGTTAAACATGTACTCAGGAA
    TGATGTGTAGGCTTCGAATAGGAGTCTGGAGTTCACATGGAAGATCATGACTAGACACACAAATTTATGA
    ATCACCAGCATATGAAAGCAGTAAAACCAGTGAGCCAGGGTGAGATCACCAGGAGAGGATCCCCAGGTTA
    GACACCAGCTCTCAACTACTGGTTGAGAAATAAACAACTAGTAAAAGAATGAAATGGTAGGTGGTGAGGT
    AGTAGAAAGACACAGATGGGGATAGAGTAGGAAAGGGCCCATGGGAAGAGAAGCAAGAACTACCCCATGG
    AGTTAACCACAACTCCATTCTTGGACTCTTTAACAAGAAGTATATTTTTAGGGAAGAAGTGGGCATATGT
    TGGTTGCAGTTTCCTCCAAGACTCAAACCTTTTATCCTCTAGAGAATCTCCTTAAGTCACAAATTAAACA
    ACTCAAAATTGATTCAGGAAGTCCCTGAAACTCAGAAGACTCACTAAGAATCAAGACTGCTGAGCTTTAG
    TCTTTAGCCAAGTTGCAAAGACAAAGGACTCTGAGATTAGACCAGCTGCCTAGAAGGATCAGAAATCAGA
    CATGCTGCCCACAAGAGTCTCAGATCAACCTAATCACCTGGAAAGGGGGCACACTTTAACCTTTTGAGCT
    GCGGGCAGGCTGTGCAGTGTTCTCTAGGTTTCCAGGTTTTATGAGCTGTCACCTATGCTGAAGTGGGCTT
    TGATGATGCAGCTGCTTTTGAGTTATTTCTGCTCTTATAAGTAATTCTCACTCATAGTTCTGTAAGCAAC
    TCAATTTTAAAAGTTGGACTTTGGTTGTTATGGTGGTTTGTATATTCTTGGCCCAGGAAGTGGCACTATT
    AGGATGTGTGGCCTTGTTAAAGTAAATGTGGCCCTGTTGTAGTAAGTGTGTCACTGTGGGGGTGGGCTTT
    GAGATTATCCTCCTAGGCCAGTCTCCTGTTTGCCTTCAGAAAAAGATGTAGAATTCTCAGCTCCTCTATT
    GCCATGCCTGCCTAGCTGTTGCCATGCTCTTGCATTGAGGATAATGGACTAAACCCCTGAACCTGTAAAT
    CAGCTCCAATTAAGTATTGTCCCTTATAAGAGTTGCCTTGGTCATGGTGTCTTTTCACATCAATGAAACC
    CTAACTTAGACAGAGTTATCCCTAGTTTGGTAGATTGTGGGTTTCATATGCAGGGTGAATAATGGTGTGT
    GTGTGTGTGCGTGTGGTCTTGTATCTCTTCAAGAAAAGCCTTCTCACACAGCAGCATAAAATTCTGGTTT
    GTCTCAAACAAGTCAATGGCCTTTCTCTATACAAAGAATAAACAGGCTGAGAAAGAAATTAGGGAAACAA
    CACCCTTCTCAATAGTCACAAATAATATAAAATATCTTGGCGTGACTCTAACTAAGGAAGTGAAAGATCT
    GTATGATAAAAACTTCAAATCTCTGAAGAAAGAAATTAAGGAAGATCTCAGAAGATGGAAAGATCTCCCA
    TGCTCATGGATTGGCAGGATCAACATTGTAAAAATGGCTATCTTGCCAAAAGCAATCTACAGATTCAATG
    CAATCCCCATCAAAATTCCAACTCAATTCTTCAATGAATTAGAAAGAGCAATTTGCAAATTCATCTGGAA
    TAACAAAAAACCTAGGATAGCAAAAACTCTTCTCAAGGATAAAAGAACCTCTGGTGGAATCACCATGCCT
    GACCTAAAGCTTTACTAGAGAGTAATTGTGATAAAAACTGCATGGTACTGGTATAGAGACAGACAAGTAG
    ACTAATGGAATAGAATTGAAGACCCAGAAATGAACCCACACACCTATGGTGACTTGATCTTCGACAAGGG
    AGCTAAAACCATCCAGTGGAAGAAAGACAGCATTTTCAACAATTGGTGCTGGCACAACTGGTTGTTATCA
    TGTAGAAGAATGCGAATCGATCCATACCTAACTCCTTGTACTAAGGTCAAATCTAAGTGGATCAAGTAAC
    TTCACATAAAACCAGAGACACTGAAACTTATAGAGGAGAAAGTGGGGAAAAGCCTTGAAGATATGGGCAC
    AGGGGAAAAATTCCTGAACAGAACAGCAATGGCTTGTGCTGTAAGATCGAGAATTGACAAATGGGACCTC
    ATGAAACTCCAAAGCTTCTGCAAGGCAAAAGACACAGTCAATAAGACAAAAAGACCACCAACAGATTGGG
    AAAGGATCTTTACCTATCCTAAATCAGATAGGGGACTAATATCCAACATATATAAAGAACTCAAGAAGGT
    GGACTTTAGAAAATCAAATAACCCCATTAAAAAATGGGGCTCAGAACTGAACAAAGAATTCTCACCTGAG
    GAATACCGAATGGCAGAGAAGCACCTGAAAAAATGTTCAACATCCTTAATCATCAGGGAAATGCAAATCA
    AAACAACCCTGAGATTCCACCTCACACCAGTCAGAATGGCTAAGATCAAAAATTCAGGTGACAGCAGGTG
    CTGGCGTGGATGTGGAGAAAGAGGAACACTCCTCCATTGTTGGTGGGATTGCAGGCTTGTACCACCACTC
    TGGAAATCAGTCTGGCGGTTCCTCAGAAAATTGGACATAGTACTACCGGAGGATCCCGCAATACCTCTCC
    TGGGCATATATCCAGAAGATGTCCCAACTGGTAAGAAGGACACATGCTCCACTATGTTCATAGCAGCCTT
    ATTTATAATAGCCAGAAGCTGGAAAGAACCCAGATGCCCCTCAACAGAGGAATGGATACAGAAAATGTGG
    TACATCTACACAATGAAGTACTACTCAGCTATTAAAAAGAATGAATTTATGAAATTCCTAGCCAAATGGA
    TGGACCTGGAGGGCATCATCCTGAGTGAAGTAACACATTCACAAAGAAACTCACACAATATGTACTCACT
    GATAAGTGGATATTAGCCCCAAACCTAGGATACCCAAGATATAAGATATAATTTGCTAAACACATGAAAC
    TCAAGAAGAATGAAGACTGAAGTGTGGACACTATGCCCCTCCTTAGATTTGGGAACAAAACACCCATGGA
    AGGAGTTACAGAGACAAAGTTTGGAGCTGAGATGAAAGGATGGACCATGTAGAGACTGCCATATCCAGGG
    ATCCACCCCATAATCAGCATCCAAACGCTGACACCATTGCATACACTAGCAAGATTTTATTGAAAGGACC
    CAGATGTAGCTGTCTCTTGTGAGACTATGCCGGGGCCTAGCAAACACAGAAGTGGATGCTCACAGTCAGC
    TAATGGATGGATCATAGGGCTCCCAATAGAGGAGCTAGAGAAAGTAGCCAAGGAGCTAAAGGGATCTGCA
    ACCCTATAGGTGGAACAACATTATGAACTAACCAGTACCCCGGAGCTCTTGACTCTAGCTGCATATATAT
    CAAAAGATGGCCTAGTCGGCCATCACTGGAAAGAGAGGCCCATTGGACTTGCAAACTTTATATGCCCCAG
    TACAGGGGAACACCAGGGCCAAAAAGGGGGAGTGGGTGGGCAGGGGAGTGGGGGTGGGTGGATATGGGGG
    ACTTTTGGTATAGCATTGGAAATGTAAATGAGCTAAATACCTAATAAAAAATGGAAAAAAAAATTCTGGT
    TTGTTCAAAAGAGTCTAAGAATAGGGAAATCTCAGATACATTTTTTCTATATGGTGGGATAAATGACAGA
    TATAGTGAGAAGGAATAGAATTTAAAACATTAGGCTATGTATTTCTTAATAGGAGGAATTGGAGCAGAGG
    CATGATGGCAGGAGCTCAATCTATAAGACATGATAAGGAAGCATTGCCCATTCAAGCCATACATACATTC
    AGTTCCACTGATCACCAGGAGACCCTCCACAGCAGCCAAACTAGGTCCATTACAGCTCCCACTCTGCCCA
    GTTAGAAAGAAGCCCAGGGGAGAGGTTAAACTAGATCAGGCATTTCTCTGAGCACACTATCACTTGACAA
    AGGGGAGTAAGACACAATAACTAAAGGAACTAAAAACCTACACTCACTATGTGTAGAAGGGATTTGACAG
    ATGAGCCTGGTTCTCCTTTCCTGATGCCTGAGGAATGACCCCCAACACACACACACACACACACACACAC
    ACACACACACACACACACACACACACACACCTATAACCCTTTCCCAACATAAACCATAAAATACAATTGA
    AAAAACATACATTTATGAAGAATTTCAGCAGAGAGACAGTTCCTTGATCGCCTCAAAGCTTGGTCATGAT
    TTCTCAAAAGCTGTCAAGTCTTTCACAGTTCAACTGGAAAGTTCAAGAGTAAGCTGTCCATCTAGAACTG
    TGACTCAATGTTATTGGCCGCCAACAACATTGCCAGGGCCTCTTTCTGTTCACAACAATGAGTTCAGCTC
    CCTTCCCCCACCAGTCCTGTGGTCATTTCTTCCCTAGGCCCCACCCTTGGCCATTTTCAGTTTCTATTTC
    TTCATTTGGCAAAATAATTTTTTATCATCATCCTAATGTGGTCTGTGACCTCTCAAGAAGACAGAAGAGA
    TCAACATTTGGAGAAAACACCCATCTTTTACACTCAGGTACATAGATCACCATCATACTGTCTAGTTCCG
    TGCCCTGTAGCGGGATAAAGCACTTAAACACACATGCGTACAACACTCGAGTCTCCAGACAGAGAATGTG
    TATGATCACTGCTAATCAAATGGCTCAAGTTACCTCACTTTGCTAATTCTGACCTCAGCCACTCAACTCC
    TTAATTTTCTTTTGACTTTTATCATCATGGTGTGTGTCTGCACGGTGGCGGTGACGGGCTCATGTGGCAT
    GGCACATATGCAAGTGAGGTCAGAGGACAACTTTATGGAATGAGTTCTCTTTGCACCTTTGAACTCAGGT
    CACCAGGCTTATGTAGCCAGCTCCTTTACTCACTGGCCAAGCCACCTCACTGGCCCAGGATAACACTTTA
    GAAAGGGATAACATTGTTTAATATACAGCAAAATAATCAGTCAAATGATGGCCAGTGTCTGCTTCCTGGC
    AGAGATGGTTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTCCTTTC
    TCACCATACTAAACACAAAGAACATGACAACCTACAAAATGAGCATTGGTAAGAGTGAGCCTTAAGCTCA
    GCAGCAGATCCTACTCCACTAAGGAGGGACGGCAGCACCACCATTACCGTTTTCCTTTCAGTACAGTAGG
    AATAAACGTGGAATGGCATTAGAATTCAACAGGACTATGCCTAAGACAAGCTGGCATGGTGCCTTCCTCT
    TAGTACTTTCAGGTTCACACTAGGTTCCTTCTGAACATGTTGTTTTACTTCAATATGTTAGGATTTCTAG
    GCTAACATTGATTTTTCCATCCAACTCTAATCCAAAAACAATTGCAAATAGCCCTTCTATCTCCTGACAG
    CTGCTAACATATTCACAAACACACATATACAAATCTATAGGATATATCAATATATGTATAAATGCATATG
    TAATTTTGATATTAGTTAACTGAAACACCATAAAATAATTCTTCATCGATTGCATGCCATTTCTCCCTAA
    CAACACTCACTTTCACTCAACACCCTGATAGACTTTTTCCTCCCACCAGTGCAAAGGCAAGCACAAGCCT
    CACACAATGTTGAGTTGCAGCCTCAGGTATGATGTAATGGGAGATTCTTCTCCTCCTATCTCTCTTTGAG
    TCCTAGCTTCCTGTGTGGATGGATGGAATGAGAAGCAGAGAGATTCAGATACAGAAGTCAATCCAGATTT
    ACCCATTATGAGTCATTATTTTAAAAATCTGATACATATTAGGCAGCTATTCCCATTTAGAGATCATGAA
    TTTCTCTAAGGGATCAAATGCATACAGATATTATGTGATTATAATCCTTAAATATATAATTAATAATATC
    AGCTATAAAAGTAATCAGAAGCCAAAATTCTAGCAACATTGGCCCAAAATAAATTAGTAACTGTCACTGT
    CAAAACTGTGGGGAAGCTGTTGAATAGGAAATTCTTTCATTTTTATCAGCATTGCAATCTTAGCTCTGTC
    TGGGGTGGAAGAGATTAACCCTCCCAAGACCTGTTTCCCTGCAAATGAGTTACTCTAGCAAATGTGCCAT
    ACACACGAATATTTATAAACAGATGTCTAGCATGACACTCTCTAAACACATGTGCACTTAGAACAATATT
    GGGGGGTTAGAATTTAAGTTGTTGACTGTTATTTATGTAACAGGCTGTGGGATGAAGACATTATTTCCTG
    GGTTGTGGTTAACCAGCATTCAGAAGAAAAGTTACATAAACAGAGACCAGATTCTAAACGTTTTAAAGCC
    TTGGGTCAAATATCCATTTATAAAAGCTGATTGGTGTTACAAATAGTTCCCTCAGGAAAATCCACAGAGC
    TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTAGCTTCCCTGAAATCACAGAGCTGAAATCACAGAGCGGCA
    GCACACACCTCAGATGAAGTGCTTCCCAAGGTCAAAATTCTGCTTCCAAATTGTTTCAATAACCTTAAGC
    CATTTTGCTAAAACTCTTCAAAATTAGTTTATATATATATATATATATATATATATGTATATATATATAT
    ATATATATATATAAATCTGTGTCCTTGACAAATGTTATATTATGCAATATAGCCACAGACACTTCTTAGA
    ATTGAAACAAGATTTAACTAAGAAAGCACCATTGTATTTTTCCTTTAACCCACCAGCAATCTGCAGGAAA
    ATTCTAAGAAGCAGCTTCCCACCGCACTGTACACTGCCTCTGCCTGCCAATCAGCAGCCAGCAGTAATAT
    CTAAACTACTAGAAACCTGTCAGGGATCTGAACTAGGGAAAACCTGACCCCCTATCATGTTGCAAAACAG
    ATCACAAGGCAAGAGGAGCCATTAGAGTGAGAATGGGACTGCAAACTCCCAGAAGGGAAAAGGGGAAGCA
    TAAAGATCAGAGGAGAAGCAGACACATTTATAACAAGCCTGAAGCAATTTCTGACCCTTCCACATTTTAA
    GTAAAACTCCGGAGTTTGTTGTTGTTGTTGTTGTTGTTTTTTTATTCTAAAAGCCTAAAATAACTATAGT
    TTTGTCTCTACTCCTTTATTAATATTGATGTTCCCTCTTGAGTCGCTTCTTTCTTATCAATCCCTCTAAA
    CTTCCTTTAAGAAATCCTTTAAATTTTAGTGAGCATTCAATGTTGATAAGGATCTAACTTGAGGCAGCAA
    TCGTAGTAATCTGTTTATTTACCGGCTCAGACTGGAGAGTCCTGGCAGGTCAATGGGGCCTCCCTGGCGC
    CACCAATGAGAGTATCAGAAATCAATTTAAAATCACCAGTGGAAAGTAGCTGCTGCCAATCACCTCATTT
    CCATTTCAGCTTCTGCGGGACTCTGTCTCTGGTTCTGTCTGAAACCTGTCAGGACGCTGCCTGGTTCTGT
    CTGGAACCTGGATCATTAACATTGTTTGGGCATTAACATAATATTAAATGCAGGGGGGAGGGGCTATAGA
    CATTCTTTTGAGCTTCCCTTTCTTGCAACTTTGCACTCTCCAACCCACTTGAAAAAGTTAGCTGGGTTTT
    CATCTAAAGCCCGCTTGAGTGAGGATTTTATTTCAGGACAATCTTCCCCGTTGCTTCCCTTCAATCTGGT
    ATTTCATACAGACAACTGCTTTGATTAAGCCTTGAGCTAAGCTGTTGCTAATACAGACCCAGACCTATGG
    CCATTCCTGCACATTTATTTCCCTAGAAAACGCATCTTCAACAAGGAAAAGGTGAGTAATTTTTGCAAAG
    GGAGAGAGGAGAGAGAGAGAGAAAGAAAGAAAGAAAGTCCATAGCAGCCATCCATTCGCCGGGGCCAGAA
    GTTTGAAAGAATGTGGCAAGCAGAGCCGAGTTAGTACCTTTCCTGACCTCTCTGAGCTGTTAGATGTGGA
    ACTCAGCCTTCCAAACACAGCAGGATCCCGTCTTCGTCCAACTCTGCAACTGGGCGGGGGCAGTCCTGCC
    CTGGCAAGGTCCCTGCAGCGCGTAAGGAGATGCACTGCAAGTCTTTTGGGCGCAGAGAATTGGGAGAGAC
    TTGCAGGAGGACCTGCAAGCTGCCCTACTCTGCAAGGGGGAGGATGAAGAAGAGAACTCTGGGGAAGAGT
    GGCTGGACAGAAGGGGAATACAGTGCCACTGAGTATTGAAGTCTTGGTCAAGA
  • Protein Sequence : Show Sequence
    >gi|363000984|ref|NP_001241676.1| interleukin-15 precursor [Mus musculus]
    MKILKPYMRNTSISCYLCFLLNSHFLTEAGIHVFILGCVSVGLPKTEANWIDVRYDLEKIESLIQSIHID
    TTLYTDSDFHPSCKVTAMNCFLLELQVILHEYSNMTLNETVRNVLYLANSTLSSNKNVAESGCKECEELE
    EKTFTEFLQSFIRIVQMFINTS
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): MVA/IL-15/5Mtb vaccine
26. Il17f
  • Gene Name : Il17f
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 257630
  • NCBI Protein GI : 119360346
  • Genbank Accession : AC159614
  • Protein Accession : NP_665855
  • Taxonomy ID : 10090
  • Chromosome No : 1
  • Gene Starting Position : 20777145
  • Gene Ending Position : 20784269
  • Gene Strand (Orientation) : -
  • Protein Name : interleukin 17F
  • Protein pI : 8.94
  • Protein Weight : 16848.95
  • Protein Length : 161
  • Protein Note : Also known as C87042; IL-17F
  • DNA Sequence : Show Sequence
    >gi|372099109:20777145-20784269 Mus musculus strain C57BL/6J chromosome 1, GRCm38 C57BL/6J
    ATTTTTGAATTAAAAATATTTTATTATATAATTAATGGATATATTAATTTTCTACTAAATTGATATATAA
    TCAATAGGTCATAAATATATGTATAGTTCCCCTACCAACATAAATATATAATAGTTTATGCCAACTTCAT
    TTATATTTACCAAATTTTTCTCTAAAAACACACATACCATGTGTGCTTCTTCCTTGCCAGTCCAGCCCCT
    GCTACACAGGCCTCCTCCCATGCATTCTGATTATGTTTGTTTTCTTTCCATCACAAATATGAGCATTTCA
    TTGAACTCTACAAGAATGGTACTGCATCAAAGTGACAGTCTTGACAGACAAGAACTGTGGTCATCATCTA
    ACACAAAGAGACTGGGCCAAGGGTGCCTTCATTTACCTTTGTTTTTAGGAGTTTAAGTGAGGCATTGGGA
    ACACACCAGGACTGGTAACAATTCTAAAGCAAGCCAGAAAGTTCAAGCTATATCTACTGCAGAGCTGCAA
    GAATGCCATGCACACCTTACTGAGAGTCCTAGAAAGCATAAAGACTTGGAGATCGGGCTTCACACAGAGC
    TCTGGATAAAAGGAGGCTTTTTCTCAGTGGAAGTCTTCAACTGATTTTTGTATGCAGCGTTGTCAGGCCG
    CTTGGTGGACAATGGGCTTGACACAGGTGCAGCCAACTTTTAGGAGCATCTTCTCCAACCTGAAGGAATT
    AGAACAGCCCTGGGGCTCCCTCCGAAGGACCAGGATTTCTTGCTGAATGGCGACGGAGTTCATGGTGCTG
    TCTTCCTGACCCTGGGCATTGATGCAGCCTGAGTGTCTGCACTGGGCCTCAGCGATCTCTGAGGGGAACC
    GGTGGGGGTCTCGAGTGATGCTGAAGGAAAAAAATAAAAAGTAAAGCAGGATGCTTGAGGCCAGGGAGAG
    GAATAGAGAAGGGTTTCCGCAGCACTAGGTGTTTCCCTTCAGGGGCAGTTCTGAGTTATGCAGGGAGCAG
    CGCAGACTCTGAATTCTTGGACCAAGCTCTAGCTGATAAGTAACTTCCTAGACCTCAGATGCCTCCACTA
    TAAAACGGGGTTTCTCCCATATCATTCTCTCAGAGAGTTGTTGGGGATATCACATGGTTTGAATGCATAC
    AGAGTAACCCCATAAATACTTTGGTTACTCTTAAATCTACATTTTCTTTGCATTAGTGTTATTATTCATC
    CTTTAAGCAGAGGTTGAACATCACTAGTAGGTCTTCCAAAGTACAATGCATAAAACTCACTGCTTGGAAA
    GCAAAATGAAAGCCTTCAAGGTCTGTGAATCCAGAGCCTCACTTGGTTCCTCTCTTGAGACTCCCCTCTG
    GCTGAAGACAGGCAGCCAAGTGAGTCTACCACAGTCTACAAACCTCTCAAGAAGGGAGCTATGTATCAGC
    CTCAGAGCACTGCTTTGTCCCTAGAATCTGTTTTTATTCGTGTATTTGAAGAGTATTGATGGGTGGTGAG
    TGTTCCCAGGACACAAGACTCTGCCCTATACTACATCTATTTCCCAAGCTGACTTTTCTTCCAAATCCTG
    ACTTACCATCCCTACTCAAAGAAAATGTCTAATCTCAGTAGATCCCTAAACGGATTCATTGTTTCCACAT
    ATCCTCACAGGCTGAAAAAAATATTAAGACTTCCAATCACATAGGTGACCCAAGGCAAGAAACTGAGTTA
    GTGGGAAAGGTGCAACTTCTATTGGAGACGGTGAATGTGAAGCCAGGCCTGGTGGCTCATGCCTGTAATC
    CCAAAACTCAGAAGACTGGGGCAGAAAGGATAACCATGTATCTGAGGCTAGCCTTGGCTACCTCATAAGT
    TTCTGGGCTACAGAGTGAGGCCTCTATCTCAGAAAAAAAAAAAAAAGTGAATGTGAGTCCTGAATACTCA
    TAAGAATGAGGCATTGTAATCAAAATAATGTAGCATGTTTTTCTTTCTTTTCTTTTATTATTTTGGTTTT
    TCAAGATAGAATTTCACCATGTAGCTCTGGCTGTCCTGGAACTCATTACATAGACCAAGCTGGCCTTGAA
    ATTAGAGATCTGCCTGAGTCTGCCTCCTAAGTGCTGAGGTTAAAGGCATCCACCATTACATTGGGTTTAG
    CACATTCCTTTCCATGTAATGTGTCTATCTCTCTTCCTTGACTTACTTGTAATCCCATGGGGAACTGGAG
    CGGTTCTGGAATTCACGTGGGACAGAAATGCCCTGGTTTTGGTTGAAGATTCGAATGTCAACTCTCACAG
    TGTTATCCTCCAGGGGAGGACAGTTCCCAGCCTTCTGCAAGGCAGGAACCCCTGCTTTGGGGTTCTTCCG
    AGCTGCTACCTCCCTCAGAATGGCAAGTCCCAACATCAACAGTAGCAAAGACTTGACCTGGAAAATAGAG
    ATTACCACAGGTTAGAGCCCTGGTGGTGGGCAATGTACTTGAACTCTGTATAGGGTCACTTTACAGGATC
    AAGGTTTCTTTTTTTTTTTTTTTTTCATGGTTCACTAGCAATGAGAACTGCCGTGTTTAATGTTATCAGG
    ATACAAAGCAGAAAGGGTCAGGAGTAAAGCTCTCTGGAGTTTATCTCCGGCCTTATAGTCCACAAAACCT
    TTCTCTGGGACATTCCACCAAACCTCACCCTACTGTCAAGGTCCCATATGTAATTCAATTAGAAGTATAT
    TTATTGGGATGGAGGAATGGCTCCACAGCTAAGAGCACTGATTGCTCTTCTAGAGGTCCTGAGTTCAGTT
    CCCAGCAACCACATAGTGGCTCACAACCATCTGTAATGGCATCCAATGCCCTCTTCTGGTGTGTCTAAAG
    GCAGAAACAGTGAACTCTTATACATACAATAAATATTTTTTTAAAAGTATATTTATCAACATTAAATAAT
    AAAGATTTTAGCATGTATCACTCAAGAAGTATTTTTTCTGTTCTCTAAAGATGTCATATCTGTGTTCATA
    CCAGACTCAGAGTTAACTTCCTGCGAGAGCATAGTTGGTCCATGAAAACTTTCTGAATGAATGAGAGAGT
    GGGGAAGTGAATGAACAGGGCAGCCCAGTTAAATTAACCAGTATAGTTATGCCACGCTCATTGAGTAAAA
    GGATTATGGGAAACTAAGCACAGCAAAATTTCATTTATCCATGCTAATTGAAAGAAGCAGGGACACTGAT
    GACGCCTGGCATCACTCAGAGTGCAGAACCCATTTCTTTTAGCTCTAGAATCTATCTTTTCTGTACTAAC
    ACTGAAATCAGTTTCGCTGCTGTTACTAGCGCACACAAAGTGCTCAGTGGAGCTCTAAACACAAGAAGAG
    CTAAAACCTCAACAGCTCCCCTTGGGTGCAAATGTCCTTTGCCGTCCCTGCTCACACCTTCCTGGGGAAG
    AACAAAGCCCATGGTAAGGCAGAAGAGCTAAGGCTGAGCTTTTCATTTCTGGGCTCCGTGTTGTTCAGGC
    TCACACTGTGTAGGTAGGGAAAGTGACTGTGCATGTTGGATTTAAAGATTTGCCTGTTCTTTTCAACATC
    CTGGAGGCAAGAAATATGCAGAATAAACAAAGAGGTCATGGAGTCAGCCTGCTGGACTACGATCACTGAT
    AATACATTGAAAGCAAGCTGCAGGATGAAACAGATTTGTCTCCAGATAATCAGCAGCCATATGCCTTCGC
    CTCTGAAGTCATTCGTTCACTGAGTAGCTAATCCTCGAGGCCACAGTGTTGTGGTTTCAGGCATTATCTC
    TAGAGTTAACTGTGTGGAGTCCATAATTCATTTATAGGACTCACCGCACAATGACTTGGTTTCCTACATC
    CTCATAAATCCCAAAGATCCCTGTAAGCATGCAGTAGTTCCACAAGGCAACAACGGTTGGCAGTGTATTG
    ATGTTACCCTGTGCATGGGATGGATGTAAAATGCAGTCTTCACAGGTGCTTTGAAATTTAGAAAACATAG
    TCACACATCTATCAACCACTTCTTTAGATTTCTTAAGTAAATTTCTCCTCCAGAATCTTCTCTTTCTTCT
    TATATGTCATGTTTATTGTCTCACTTCTCATTTTGACTAGCAAGACTGAAAGTCACTATAGTTCGGGGTT
    ATGTGATTAATGTAAGTTGTTGTGGTGGTTGAACTGCGAAGCAACCTGCATAGGCTTATTTATGTGGATG
    CTTGGTCCCCAGTTGGTGGAATTATTTGGGAAGGAGTAGGAGGTGTGGTCTTGTTGGAGGAGGTGTGTCA
    CTGAGGTGGGCTTCGAAATTTCAAGTCTTATATCATTCCCAGTTAGTTCTTTCTGCCTCATGGTTATGTC
    TTAAGATGTGAACTCCCAGCAACTTCCCCAGCGCCATGCCTACCTTCCTGCCTCCAGCCATGATGATCAA
    GCAGCTTAACCCTTGGCAACTGTAAGCCCCAAATTAAACACTTCCTTTTATATGTTGCCATGGTTACAGT
    GTTTTATCACAGCAATCGAAAAATAACTAAGACAACGATCATGTCCCTAACAGCTCATTATGTTTCTTTA
    TTAATAGCTAGACATTCTTGGAGTATTTTAGAAAATGTTTTCACACAAGATTTGAACAGCTAACTCATAG
    ATGAAACTCATCCTAACTATCTAATCAGCTCTCAAAGGACTTATGTCAGAGAGGCAATAAACAACCCGGC
    AGGGTTCCCCAGCCTCATCGTGACAATCTATAGGATCCACCTTCTATGGCAAGGCTTGGCCACAGCGACT
    GAAGGTCATTACTATTCTTCACATTAGTCTTCACTGTGTCTAATTTCAAGAGCCACGAATTCTAAACACT
    CTCTGCACTGCATCAGTAAGACAGAAGTATTGAAAGCCACAAACCACTGTTTGCTTTATGCGTTTTTGGG
    GTTTTGTTTGTTTGTTTGTTTGTTTTTTGGTGTTTTGCAGCTCTGATAAGGCTGGTGCTGAGTATGGAAA
    AACAATAGAAACAATCCATCTTCTCAGGGGAGTCGGGAGATTTTACAGGTTTAAATTCCACCTTTAAAGG
    GGATTGATAGGTGTGATTCAAATCCTGTAGGATGGGAAACAGCTAGGAGAATATTGAAACTGAAGGTGTT
    CAGTCATAGAGTGTGAATCTTCCCAGCTTAGCTTTGTACATTACTCTTGTGTGCTCGCTGGTGGCAGAAC
    ATCTGCACACTTGCCCCTACACTTTCCTCCCTACACAGGAATGGGTGACAGAGACTGGTCAGCTCCAAGC
    TCCATCCAAATTTCCCTCAATTGCCCACAGCATATGTCTTCTCTGCTTGCCCCTCCTCATAATCCCGTTT
    CAACACTTCTTGCTCCACCATGTTTCTTGCTGACAATCCAACTGATTTTTTCTCCCCCAAAGCAATAGTA
    ATGGTTTGGAGCATTAAACTATCCAAACGATTTTCTTGGCTACAATGGCATTAGGTTGAGAATCTTGCCT
    CTCTCGATCATCCACAGATAAGCACACTATTGTTGGGAGTCAGAAAATCTAGATGCAGGTCTCAATATTA
    TTATATTAGCAATATATTAGCATTGCAATAGAAAATGACAGCAAATGAAAACAGATGACTGAGCTACTGT
    GTAATGGAGAAAATGTTCGTGATCTATTCCTGTGGGTTGACAAGGGTCTGAGAGGTAATAGTAAATCACA
    GAGCAAGGCTTGGTGGCACACTCCTGAAGTCCCAACACTCCAAGAGAGCAAAGCAAAAAAGACAGTTCCA
    GAGCAGTCTGAGCCCACATAGCAAACTCACGACTATCGTGAGCCACACAGAAGATCTTGTCTCAAGGGCT
    GGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCCTCCAGAGGTCCTGAGTTCAATTCCCAGCAACC
    ACATGGTGGCTTACAACCACGTGTAATAGTACCTGGTGCCCTCTTCAACAGTGTATTCACATACATTAAA
    TAAATAAATAAACAAACAAATAAATAAATAAATCTTTTTTTAAAAATCTTGTCTAAAAAAGCAAAAACAA
    TAATATAAAGAATTGGTATTTGTATACTCTTTCTACCTTTACCTCTTATAGCAATTGCAGTGTCCAAGTT
    AACTAAAATCTAAGCACTTATGATCTACCTTGCAAGTCTAGATTCATATCAATATAACAATCTCTTCCCC
    CATAATTATGCACAGGTAGGGGAAGTAAAACTCACAAAGGGAATGACCAACACCAAAGTGCATACTTAGC
    AGGTAGGAGAGACAAATCGGTCTGACTTCAAAGCCTCTGTCTCTAAATACTGTATTTTGGTGGTTTACTG
    CAATCACTATAATACTATACACTATGCAGTAATTAAAAGAATATGGCCATTGTCGGAAAGCATGAGCCAT
    TTATAAAAGCCATGCTCTTTCAGGTCTCAAGGCTTAGCCAGGGAAAATCAAAGGACTATCAAAACCAATA
    ACACAGTGTGTGATTTCCTAAGCTTACTCCATGTGCCTCTATATTAAACCCTTCCCAGGTATTAATTAAT
    TAGTCAGTTACCAAAGTCCCGAGACACTTCTCCAGGAGCTGAGCAAAAGTTGAGCCCTGGGCTATTCTAC
    TTTCTCCTCCATCCAAATGACCTTGGAGGAACCAAACCATGGGTCCCAGCTTCTTTGTGGTATATATTAT
    ATATTAATATTTAATGTATATTTATACTACTATCGCATGCAATGTAACACGATATTATATATAGATTCCT
    GCCTTGGAATACATATTTATATAAAACAAAGCTTGGCCTGGCTCTTATTTTAGGTCAACCTAAGTCTTTA
    ATGAGTAGGTAAGAGAGACCATGATGGTCACTGGAGATAACCAGATACACACACCAGCCCACCTGAGCAA
    ATCTATCAGGTAAGATCATCTGCTCTTCTCCCATTCTAACTGTTCCATTGATGTTGGTTTTTATTCTTCT
    CTTCTCTTCTCTTTTTTCTCCACCAGACAAATCAAAATTAAACTTAACTTCACGTTTTAAAGAGCTAATT
    CACAAAAGACTCTAAGAAGGCCTAAAGCCTTAGAAATCTCCCTTTCTTAAAGCTTAGACGCACCTATCCT
    TAGCCTATGATTATTCAACACCACTCACCATGGCTGTTTCACGGGTGCACTTCATGCTGGTTTCTCCAAT
    GGCTGCTTCTTCCCTCAGGAAGTACTTTCTTCTAAGTATGTCTTCCTGGGTATGT
  • Protein Sequence : Show Sequence
    >gi|119360346|ref|NP_665855.2| interleukin-17F precursor [Mus musculus]
    MKCTRETAMVKSLLLLMLGLAILREVAARKNPKAGVPALQKAGNCPPLEDNTVRVDIRIFNQNQGISVPR
    EFQNRSSSPWDYNITRDPHRFPSEIAEAQCRHSGCINAQGQEDSTMNSVAIQQEILVLRREPQGCSNSFR
    LEKMLLKVGCTCVKPIVHQAA
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): MVA/IL-15/5Mtb vaccine
27. IL2
  • Gene Name : IL2
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 3558
  • NCBI Protein GI : 28178861
  • Genbank Accession : AC022489
  • Protein Accession : NP_000577
  • Taxonomy ID : 9606
  • Gene Starting Position : 123372624
  • Gene Ending Position : 123377649
  • Gene Strand (Orientation) : -
  • Protein Name : interleukin 2
  • Protein pI : 7.95
  • Protein Weight : 17130.78
  • Protein Length : 153
  • Protein Note : Also known as IL-2; TCGF; lymphokine
  • DNA Sequence : Show Sequence
    >gi|224589816:123372624-123377649 Homo sapiens chromosome 4, GRCh37.p5 Primary Assembly
    GTTTATATTTATCAAATTTATTAAATAGTTTTACTAACCAATCTACATAGATACTATATTTAACATTCAA
    CATAATAATAAATATTTTGGGATAAATAAGTGAAACCATTTTAGAGCCCCTAGGGCTTACAAAAAGAATC
    ATAAAAGATCCATATTTATAGTTTTAAGATTAAGAATAATAGTTACAATAGGTAGCAAACCATACATTCA
    ACAATAAATATAAAATTTAAATATTTAAATAAATAGAAGGCCTGATATGTTTTAAGTGGGAAGCACTTAA
    TTATCAAGTCAGTGTTGAGATGATGCTTTGACAAAAGGTAATCCATCTGTTCAGAAATTCTACAATGGTT
    GCTGTCTCATCAGCATATTCACACATGAATGTTGTTTCAGATCCCTATAAAAGAAAAATGTTAATTTTTT
    AAAGTACAGAGTAGTTTACCTTATATACAGTTATTCCCAATTGAAGTCTTATAGGCCTGTTGCCTTTATT
    TTCAAGCTTACCAAACATATTAATTATTCACATTTTCTTGAAATGATCAAAATGAATGCCAAAATTACAT
    ATTTTGTTATGATACCAAATGATAGTAACACAGAAGCTGAGATTTTCCTCATTTGTTAATTCAGTCAATG
    AACACAAAAATTTTTTCCCTAAATTGGGTGCAAATAAAGATTTAAATAAAAATAGATCTAGACAAATATT
    AAATGCTGGATGTGAAATAAACTTGATCGCTTTTCCTCTGAATGTACACCTATATTTGTGTAAGTTAAAT
    GTATGAATTGATATGCACATTATACTTTCTTCTTTTCTAAATCTTGATTAGAGTCTCCTATTTTTCTACT
    TAAGTGAATCTCCTTATATTTTCCCCAAAGCAGAACAGAACTACACTAGAGTAAGCTAGGACATGCTTCT
    GTTTGGGATAAATTTGTGTGAAGAACATCTTCATAAATACTTACACCTATCCCTACCCCATCATAGTATC
    AATGCAGGTGAATTTGCCAACTTTTAAAAGTTTAAACATTAGAAATTTTGTTCTGTGGACTGGCTTTTTA
    GAAATTAGGCTTCTCTGCTGAAAGGAGCTATTTAAACGCTTCCTCTAGTTGATAAGCTAAGGAGGAAATA
    TTTTGATATATAGTGAGATTTAAAAGAGAGCTTTTCAAATTTGAAGTGATTCCTACCCTGGGAACAAAAG
    CAGGTGAGATAGAGTGTTCCCTTTAAATAAGTATTCTCTTTTAAAAAACTGTTGCAGTGTTCTGTATCCT
    CTTAAGCATAATTGTTTAAATATTGTCATATCTTGGGATTTTTTTATTTTAAAAAGTGTTTGGCTTTTGA
    AACACAGTTTTCAGATTTTTGCATGGTTTTGTCTTAAATTTGTATCAAGAGTTAAGAATGAGTTTTCTGA
    TGTTAATTATTTTAGAGTAAAACTCTTGCCATAGATAAGGAACTGAATCTGTCAATGTCTAGATGACATT
    GATCTCAATGTATTTTTTTAATTCAACTGAAACAGTCCACTAATAAGTGATTCATTTGGACACAACTGGA
    GTTACTTTTAGGAATGTCTTTTACTACTATACTTATGCTGCTTATTTAGGATACCTATTAACTCATGAAT
    TTTATACCTTAGGAGACGGGATATAAAAAGACAAAAGTGAATCTCTTTTACATCACCTGAGTCCCTTGCA
    TATTTTATGCTTCCTGTGCATTTAAAAATACTTAAGATTCTGAAAGTGGGGATTGCCTCTAAAAAGATTG
    TTGTTTGTTTAGTCCACTGGCCATTTTAATGGCTCCAGTTTTTTTTTTTAAGACAAATCTCTTATGGGTA
    GTCCTTCCATCAAATGATAAGGAAACACCATTTGAAAAGCCTTTCAGGTAATCAGTATTGGCTATTCCAG
    TAAATGATCTGAAGGAATTGAAAAATATATAGTAAAATCTGCCTGCTTTCTGTGAAACTCAACTCAGTAC
    CTTCTCTCACTCCCAGTTTTGTGTTAATATGAAAGGTGATGGAGTTTGGGATGAGGCTTGTCCATTAGTA
    GATGGTAGGTGCAAACTCAGTAGCTTATACTCCCCAGTTAATCATTTAACATTAGAAATGATTCATGTTC
    ACAGTGTACTTAAAATGTAGGCTAATTACATGCATGGGTACTTTACAAATACCAAAAATGTTATGTCACT
    TTAAAATGTGGTACTTTTCCCCCTACTTTTTTTTTTTTATTTCCAGGAGAGCAAATAAAGTAATGCCTTA
    CCTTTAGTTCCAGAACTATTACGTTGATATTGCTGATTAAGTCCCTGGGTCTTAAGTGAAAGTTTTTGCT
    TTGAGCTAAATTTAGCACTTCCTCCAGAGGTTTGAGTTCTTCTTCTAGACACTGAAGATGTTTCAGTTCT
    GTGGCCTAGAATAATAATTATCATCAGCTCAGTTTACATAGAGGTCAGAATCAGAAATTAATTTCCAATT
    TATTTTATTTGGAGTAGCAGTATGTAGTTTTTACCATTCTCTCTGTTCTCAATGTCACTGTAAAGATGGA
    CCAGTAGAGTTTACATTATAAAACTGGAAAAACTAGGGCTCAAGTACCTATGACTAGCGTTAAGTGATAG
    TTGACTCACTGGTTTTAGCTTAATTTGGCTTTGCAAAATGTTAGACTTTCTGCATCTAAATGCCTATTAC
    AGATGTAGGCTGATTACTTTTCCAATCTGCATAGAAAATCATTAGAACCAGATGAGTTAGAATGTGGAAG
    CATAAAAATCACATTCTATGATTATAATATTTGATAGTCTAAGAGTTAAGAATCACAGAATGTTGAGAGA
    GAAGGAACTCTTGGATGCCATCTATCACAATCCCAACATTTTACACTAGGTAACAATAATCATGTAGTAG
    TGGCTCTCAATATAGCCTTGAGCAAGACACATTTTTAAATATGTCTTAAATTTTTACTTTTCTACAATCA
    CTACATTAAGATGCATTTTAAAAATGTTTGTCCCAGTGAAATAAAAAATTATAATACTTTGGGAGGACTT
    TTGGACAGGCCTCTTGATTCTATATTATATATAATATTTCATATTTCTATGGTATATTACAATTTAAAAG
    AATTTTCTCCAAAATAGGCCCATTTACTTCTCACAGTCATCTTTTGAAGTAGGCAGTGAAGTATCATCTC
    CACTTTATAAATAAGCACACTAAATCTCAGAATTTGACCACATAAGTAGGTAGAGCCAGAATTAGATTTC
    ATTTCTTTCTCATTATAGCATTAACATAGCATCCTTGGAATCAAATATTTTTATTAGTTTCTAAAATCTT
    TTGTAAATGTGCAATGAGGGTTAAATGAACAAATGAATAGAGTTTATCATTACTTTTGGTTTCAAACTTA
    GATTTCCATGAATTCTACCACCCCCTAAATTATGCACATAATCTCATCTTTCTAACACTCTTTAGTGGGA
    AAGGTAGGTCAAGACAATACCAATCCCCTGATATGTCCATCTATTGCGCTTTCAATTCACCACTACAAAT
    TCTACAAATTCGGGTTTAAGTTTTTGGCATGAACAATGTGGAGAAATTTTCTTCAGAAAACAGCATCTAG
    TATCTTTATGTTGCTGGAGTTGCATTATGTGGCTCTGATTAAACTAGAGTAACATCTGTAAGCTGTCTCC
    TTTACATCTTTCAGAAGGGTAGTTAGAAGCAGATTTCTCTTGAATTAATAGAATTTGAGTCAATGGGATT
    GCATCTGTGTCCCCTTAAATCCTATTATTTTCTGGGCTTGGATTCACCCTGGCACAAGCTCTTTCTGGCA
    CCAGATTTTGTTCATTCTCTCTTAACTGGCACAGCTACTAGGTAGCTTTACACACCTAATGTAGCCTAGG
    AGCTTGGATGATTATGCTTATGCTCTATCTGAATCCAAAAACAACCTAATGAAGCTTTTAATCTTGGAAA
    ATAAGTCATATAGCATTATAAATCTCAAGTTCTATTGGTAAAAAGATAAAGTCCAAAATTTTACTTTGTA
    TTTCTCAGTGAAAATTTTATCTTTAATACTACTAATAATAGCTAATGCTTGTATGGTATTTACCTTATGC
    CAGACACGGTTCTGAGTGCTTTACATTTACTTACTAATTCTCACAAAGCCATATTAAGTATATACAGTTA
    TTCATTTAAGTAGACATGAAACTGAATCACAAACAGAGTAGGTAATTTGCCAAAGATCATGAAAGCTAGG
    ACATGGCAGAGCTGAGATTTCACTTCAGATATCTGGCCCCAGACTCTGTGCTATTAGTGTTATGCTGCCT
    CTCACTTAGAAACCTTTCCTAAAACAAGAAAAGATTTTTAAAAGTGGGCTCCTTGGAAATAGATTTTGGT
    ATTTTACATACTAAAATGACATAATCAAATATTTTAGAGTTTTACATTTTTAAATGAAACCATAAATGAA
    CATTTTTGTTTAGAATTGTCTTATCGATAAAGTGAGAATATTATTTTTATGTTTTCTTCCCACCCCCACC
    AACACACAAATATATATTAAAAATATAGGTAAAACTGTTTTAATAGAGGCTTCATTATCAAACTTGGGTT
    TTCAAAAAAACAAATTAAAGTTACTCTTTACCTCAGATGAGCTGCTATTAGTCCCATCTGTGCAAATTTT
    CATATTACTTTGAATTTTATTAAAACAGAAATTGAACATAAAATATTGTACTTACCTTCTTGGGCATGTA
    AAACTTAAATGTGAGCATCCTGGTGAGTTTGGGATTCTTGTAATTCTAAGAAAGTATAATGCATTGTTAT
    TAAGAAATGATCTCCAGCTAGATTACTAAATGTAATAATTTTAGTAAGAAAGGAAATATACTTACATTAA
    TTCCATTCAAAATCATCTGTAAATCCAGCAGTAAATGCTCCAGTTGTAGCTGTGTTTTCTTTGTAGAACT
    TGAAGTAGGTGCACTGTTTGTGACAAGTGCAAGACTTAGTGCAATGCAAGACAGGAGTTGCATCCTGTAC
    ATTGTGGCAGGAGTTGAGGTTACTGTGAGTAGTGATTAAAGAGAGTGATAGGGAAC
  • Protein Sequence : Show Sequence
    >gi|28178861|ref|NP_000577.2| interleukin-2 precursor [Homo sapiens]
    MYRMQLLSCIALSLALVTNSAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKA
    TELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNR
    WITFCQSIISTLT
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
28. Il2
  • Gene Name : Il2
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16183
  • NCBI Protein GI : 7110653
  • Locus Tag : DN-144H19.3
  • Genbank Accession : AF195954
  • Protein Accession : NP_032392
  • Taxonomy ID : 10090
  • Chromosome No : 3
  • Gene Starting Position : 37120712
  • Gene Ending Position : 37125953
  • Gene Strand (Orientation) : -
  • Protein Name : interleukin 2
  • Protein pI : 4.63
  • Protein Weight : 18506.61
  • Protein Length : 169
  • Protein Note : Also known as Il-2
  • DNA Sequence : Show Sequence
    >gi|372099107:37120712-37125953 Mus musculus strain C57BL/6J chromosome 3, GRCm38 C57BL/6J
    ATTTTTTTTTAGAGGAGAGCTTTATTTCTTGAAAACACTGATTAACATAGAGTTCACAGGAATAACTGAG
    AAGTTATTTTAGCGCTTACTTTGTGCTGTCCTAAAAATGACAGACATCTGAGCTTATTTATATTTGAATC
    ATCTAAATACTTTTATTAATGAGTCTACCTACACATGATATTTAACAATTCAATATAATAAATAATTTCA
    GATAAATAGTTTAAAACATTTTTGAGCCCTTGGGGCTTACAAAAAGAATCTTTAAAGATCCATATTTATC
    ATCTGAAGACTAGTAGTTACAAAAGATAGTAAACAATACATCCAAAAATAAATTAAAGTTAAATATTTAA
    ATAAATAGAGAGCCTTATGTGTTGTAAGCAGGAGGTACATAGTTATTGAGGGCTTGTTGAGATGATGCTT
    TGACAGAAGGCTATCCATCTCCTCAGAAAGTCCACCACAGTTGCTGACTCATCATCGAATTGGCACTCAA
    ATGTGTTGTCAGAGCCCTAAAAAAGAATATAAAATTGTAGGCTAAGGTAGCTTACTTTGCATATAATTAT
    TCTTCACGGAAGCTTTTAGGCATTTATTTTAACACTGGTTAAATATATTGAACATTCACATTTCTGAAAT
    GATCACAATTATTGGGTTATGTTTTATTATGATATCAAATGGTAAAGCATAAAAAATAAAGCTCTCCAGA
    GTTGATTACCAACGAGCATTTGATGGGAACACTGAACACTTCTGTAGAATTCAGTTGCTTGACCACTTTT
    TCTCTGGTAAGTGTACACAGCTATGCATCAATTTAGGCTTCAGGAATGGAGAAATGATACTTGTTATATG
    TTCTTTGCTAAATCTTAAGAGTCTATTATTCAATTCTTTCCCCCCCAAGTCAGAATGGGAACAACCCAGA
    GTAGGTTAGGACAAGCATCTATTGGGGGAAATTTGGAGAATTTTGTTATAGATATACCTACCCTGATACC
    AGTGTAGATGAATCACAAAGTTTGTACATGTATAAATATTATAAATTTGTTCTGTGGATTAGCTTTTTGA
    AAGCTAACCCTCTTTGCTAAAGGAGATATTTTAATTCTGTTTTTAGGTGACAAGCTGAGAAGGAAATATT
    TTGACAAATTTATGGGATGGCTTTTCCAAATCTGACTAATTCCTTGTAAGTCAAATGAGGCAGATTATTC
    CTTTTAAATAAATACTCCTTTTCAAATAAGCTGCTGTGGTCTTATATACCCTCTTTACATAGCTACTTCA
    ATATTCTAATGTCTTGAATTTTTCATTTTTAAAAATATGTGGCCCTTGAAACATTTTTCTAGTTCTCATG
    TAGCTCTAAAGTTTGTACCATTCATTAGGGGTGAACTTTCTGATGTTGGCCATAGATTTTAGAGCATCCA
    TCTTTGCATACGTAAGTACTCGATTTTAGAGCATCCATCTTAGCATACGTAAGTACTCACACCTTTCAAC
    GTCTAGATGATGTGTATTTCAGCCTGCTTTAAAAACCTCAAATGGAAGCTGGGCAGTGGTGGTGCAGGCC
    TTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGT
    GAGTTCCAGGACAGCCAGGGCTACACAGAGAAATCCTGTCTCAAAAAAACAAACAAACAAACAAACAAGA
    AACAAACAAACAAAAAAAACAAAAACAAACAAAACAAAACCTCAAATGGAAACAGTGCACTGAAATGTGA
    CTTTGGTTTGGAAAGGACTAGCCCACACCCTCTTGGAGGCTTGCTGCTACTGTCTCACCAGAGTCCATGA
    TTCTTGTGCATTTAAAAACAAAGCTCTGCAGTGGGCTCTCCCCGTCCTGCTCTGTCCATTTTAATGGCTG
    CCATTTTTGGAGAGAAATGTCTGTTTTTCTACCAATACCAGCACAACTTCTCTGGAAAAACTTTTCAGAT
    AATTTTTTCTGATCTGATGAATGTAACACCAGCAAGAGTTGCTTGTTTCTTGTGGAATTCTACTCCGTGC
    TTTCTCTCACATCCAGTTCTATGCTGGTGTGGAGGGAGCAGAGTGTTCATGTTCCCAGTTTCCTTGCAGG
    TGATGGTAGGTGGAAATTCTAGCATCATCCTACAGTGGAAGGATTCACTTGCACAGTGACTTTAAACTTT
    GGCTGACTAAATGCACAGAACCCATCAAAGACCAGAAATGGCAAGCCACTTAAAAATGCATTTCTTCTCT
    ATTTTATTTCCAGATTAGCAAATAAAGCAACACCTTACCTTTAGTTTTACAACAGTTACTCTGATATTGC
    TGATGAAATTCTCAGCATCTTCCAATTGAAAGCTTTTGCTTTGAGTCAAATCCAGAACATGCCGCAGAGG
    TCCAAGTTCATCTTCTAGGCACTGAAGATCTTTCAATTCTGTGGCCTAGAGGAGTAATAAGCTTAACCAT
    CAGCTCAGCTCACCACATACTGAAGAGCCAGAAAGTTAGTCTGCAGTCTCTCTAATCAAGAGAAGGCAGC
    ACATAGCTTTTACCACTCCCTGTCCTCTGTAAAGGGGGACTGAGGTAATCGATGCCATAAATCTAGAAGA
    GCAAGAGCTCACAACCATAAGGGTCACCTTGACTGTTAGGCCACTCTAGTGAGCTCTTCTGGCTTCATTA
    GACTTTGTAAAAAGTCTGTGTTTCTCTACCAATGCATAGCACAAGTTCAGACTATTGTTCCAATCTACAA
    AGAAATCTGCAAGGTTCACATTCTAATATCTAATCGCAGAGTTGAGAATCACAGAAGGGTAAGGGACAGG
    AATCCTTGGATGCCAACTGTCATAGGCCTAAATCTTACAGATTAGGTTATCATGGCCCTGACTGAGGGGT
    GTCAAGATAGCCAGGAAGACACATGTAATATTTTTTAAATTTATACTTCTCTGCAATTACTAAAGATGAG
    TTTTTTAAAAACCCATATGTCAAGCTAGGAAGATGGCTTTTAAGGTAAAGGTACCTGCCACCAAACTTGA
    TGATTTGAGTTCAATTGCAGTGACCCACCCACATTGTGGAAAGAATGGCCCAACTTTCATATTTCGTCTT
    CTGACCTCCACAGTTACACTGTGATACACATGCACCTCCACACATACATACATACATACATACATACATA
    CATACATACATACATACATACATACATAAAATGTAAAATCTATTTGTCCCAATGAAAAAAGTCATAACAT
    TTTGGCAGAACACTTGGGAGAATTTTTCTGATCCATGTATGAAATAATGTTTAAATACATCATGCATACT
    TACATACATATACAGGGAAAAAGGATATTGTCTAGCTCTATAGTACACTATGATTTCCATAAAGTCAGCT
    GCTTTAAGTCTACAGTGAACTGTGTTATCTCCATTTTACCAAGTCTCACAATCAGTGTCTTTCCCACAAC
    CACATGAACGGGGAGCAAGGGTGGCCCGGTCGGTCGGTCGCTCCTTTCATTAACCACAGCCTTTGAATTA
    CATGCTTCTATTGTTTTTAAAATCTTACATACCGAATGCAGGTTAAATGAAAACATCAGCAGAATTTACC
    ACATTACTCTTGTTTTCAATAATTATTTTAAAACTTAGATTTCTAGGAATTTCACCACTCTCTAAATTAA
    CATGATCTCTGTTGTTTCTCTAACAATCCTTAGAGGAAGAGGTTTGACAAGATAACACGTAGTTCCTGAT
    TGGTCTGCTATTGACCTTCCAACTTGTTATGGTGTGTCTTGGGTATTCAGTGTGGAGCTCCTTCCCTCAC
    AGAACTCTCCAGTTTGTTACTGTGGGAGCTGAACATTATGTGACTGTAATTAAGCTGGAAGAGCAACTGG
    GGCCTTTCTTTTTCATCTCCTGTAGAACAGCTAGAGGCAAAGTTCCCCTGACTCAATAGGAATGGGTCAA
    CAGCTGCCTCTTTGTCCCCTTAGATCCCATTTTGCTCTAGGCTTGGATTTACTTGGGACAAGCTCTTTCT
    AGGACCAAAGTTATCCATTCTCTTTTGACTTTCGGGACTAGAGCAAATTTCTACCCTTGCTGGAGTTCTA
    AGAGCTTGTGTAAGCTGTTTTGTATCACACAACATTCTGCCTCCAAAGGAGACCAAAAAACCTTTCAATA
    TTGAGAGATAGTATCATGTAGTAATATTACAGCTCTCTAGTTCCACTAGTTAAAAGAGACTGTATAAATC
    CAAAATTGTTTTATACATCTCGGACTTCTTCTCTAGCACTAATAGTGTGTTTATGTGCTGTTGGCCAGAC
    ACTGTTCTGAAACCTGACAAGTGATAAGCATAGCAATGATCATTTATGTAAGTGGGTGAATGAGCATAGA
    CAACTTCCTGAGGCCATGAGAGCTAGGCCCTAGCAGGGCTGGACTCCACTGCAGATCATTGGCCCTAGAA
    TCTTAACATTGGTTATGCTGCTCCTCAAAAATGCAGTCATAGGAATCATTCTTAAAACAGTAAAGTGTGT
    GTAAAATGAGACCCTCTCAAGTCAATTTTTACATTTAAGAAGCTGAAATAATGCACCTCTCTCAAATATC
    TGTAGGACAAGCATAAAACAGAAATCAACAGTCTTTAAATTATCCCAGCATCAAAATGCAATCATCTTGC
    ATTTTCTTCTCATTCCCTTTCCCAACACATAAATACACAGGAAAAATGTAAAGAAAACCATTTAAAAGAA
    GTACCATTGTCAAGATTTTTTTACAGAGAGATATCAGAGAAACTTATTTTCTTCAGATGAGGTACTATTC
    ATCTGGTCACATGTTATTTACACGTTATACACATGTATGTTACTTTGAATTCATTAGAGCACCAGTTAAA
    CACAGAAACTCACTCACCTGCTTGGGCAAGTAAAATTTGAAGGTGAGCATCCTGGGGAGTTTCAGGTTCC
    TGTAATTCTGAGAAAGCGTAACACATTATTATTAAAGGTTATTAAACACAGCCTTTGGCAAGAAAGCTAA
    AGGTATTGCCTATAGATGGGATGGCTGTGCACTTACCTCCATCCTGCTCAGGAGCTCCTGTAGGTCCATC
    AACAGCTGCTCCAGGTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGTGCTTCCGCTGTAGAGC
    TTGAAGTGGAGCTTGAAGTGGGTGCGCTGTTGACAAGGAGCACAAGTGTCAATGTGACACAGGATGCGAG
    CTGCATGCTGTACATGCCTGCAGGACTTGAGGTCACTGTGAGGAGTGATTAGCAAGGGTGAT
  • Protein Sequence : Show Sequence
    >gi|7110653|ref|NP_032392.1| interleukin-2 precursor [Mus musculus]
    MYSMQLASCVTLTLVLLVNSAPTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKL
    PRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFEC
    QFDDESATVVDFLRRWIAFCQSIISTSPQ
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
29. Il4 (interleukin 4)
  • Gene Name : Il4 (interleukin 4)
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16189
  • NCBI Protein GI : 10946584
  • Locus Tag : RP23-188H3.4
  • Genbank Accession : NM_021283.1
  • Protein Accession : NM_021283.1
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • Protein Name : IL-4, Interleukin 4
  • DNA Sequence : Show Sequence
    >gi|10946583|ref|NM_021283.1| Mus musculus interleukin 4 (Il4), mRNA
    GGATCCCCGGGCAGAGCTGGGGGGGGATTTGTTAGCATCTCTTGATAAACTTAATTGTCTCTCGTCACTG
    ACGGCACAGAGCTATTGATGGGTCTCAACCCCCAGCTAGTTGTCATCCTGCTCTTCTTTCTCGAATGTAC
    CAGGAGCCATATCCACGGATGCGACAAAAATCACTTGAGAGAGATCATCGGCATTTTGAACGAGGTCACA
    GGAGAAGGGACGCCATGCACGGAGATGGATGTGCCAAACGTCCTCACAGCAACGAAGAACACCACAGAGA
    GTGAGCTCGTCTGTAGGGCTTCCAAGGTGCTTCGCATATTTTATTTAAAACATGGGAAAACTCCATGCTT
    GAAGAAGAACTCTAGTGTTCTCATGGAGCTGCAGAGACTCTTTCGGGCTTTTCGATGCCTGGATTCATCG
    ATAAGCTGCACCATGAATGAGTCCAAGTCCACATCACTGAAAGACTTCCTGGAAAGCCTAAAGAGCATCA
    TGCAAATGGATTACTCGTAGTACTGAGCCACCATGCTTTAACTTATGAATTTTTAATGGTTTTATTTTAA
    TATTTATATATTTATAATTCATAAAATAAAATATTTGTATAATGT
  • Protein Sequence : Show Sequence
    >gi|10946584|ref|NP_067258.1| interleukin 4 [Mus musculus]
    MGLNPQLVVILLFFLECTRSHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCR
    ASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS
  • Molecule Role : Vaximmutor
  • Molecule Role Annotation : IL-4 plays an important role in Th2 immune response.
  • Related Vaccine(s): Mycobacterium tuberculosis fadD26 mutant vaccine , Mycobacterium tuberculosis mce-2 mutant vaccine , Mycobacterium tuberculosis mce-3 mutant vaccine , Mycobacterium tuberculosis sigE mutant vaccine
30. IL6
  • Gene Name : IL6
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 3569
  • NCBI Protein GI : 10834984
  • Genbank Accession : AC073072
  • Protein Accession : NP_000591
  • Taxonomy ID : 9606
  • Chromosome No : 7
  • Gene Starting Position : 22766765
  • Gene Ending Position : 22771620
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 6 (interferon, beta 2)
  • Protein pI : 6.52
  • Protein Weight : 22923.52
  • Protein Length : 212
  • Protein Note : Also known as HGF; HSF; BSF2; IL-6; IFNB2
  • DNA Sequence : Show Sequence
    >gi|224589819:22766765-22771620 Homo sapiens chromosome 7, GRCh37.p5 Primary Assembly
    CAATATTAGAGTCTCAACCCCCAATAAATATAGGACTGGAGATGTCTGAGGCTCATTCTGCCCTCGAGCC
    CACCGGGAACGAAAGAGAAGCTCTATCTCCCCTCCAGGAGCCCAGCTATGAACTCCTTCTCCACAAGTAA
    GTGCAGGAAATCCTTAGCCCTGGAACTGCCAGCGGCGGTCGAGCCCTGTGTGAGGGAGGGGTGTGTGGCC
    CAGGGAGGGCTGGCGGGCGGCCAGCAGCAGAGGCAGGCTCCCAGCTGTGCTGTCAGCTCACCCCTGCGCT
    CGCTCCCCTCCGGCACAGGCGCCTTCGGTCCAGTTGCCTTCTCCCTGGGGCTGCTCCTGGTGTTGCCTGC
    TGCCTTCCCTGCCCCAGTACCCCCAGGAGAAGATTCCAAAGATGTAGCCGCCCCACACAGACAGCCACTC
    ACCTCTTCAGAACGAATTGACAAACAAATTCGGTACATCCTCGACGGCATCTCAGCCCTGAGAAAGGAGG
    TGGGTAGGCTTGGCGATGGGGTTGAAGGGCCCGGTGCGCATGCGTTCCCCTTGCCCCTGCGTGTGGCCGG
    GGGCTGCCTGCATTAGGAGGTCTTTGCTGGGTTCTAGAGCACTGTAGATTTGAGGCCAACGGGGCCGACT
    AGACTGACTTCTGTATTTATCCTTTGCTGGTGTCAGGAAGTTCCTTTCCTTTCTGGAAAATGCAGAATGG
    GTCTGAAATCCATGCCCACCTTTGGCATGAGCTGAGGGTTATTGCTTCTCAGGGCTTCCTTTTCCCTTTC
    CAAAAAATTAGGTCTGTGAAGCTCCTTTTTGTCCCCCGGGCTTTGGAAGGACTAGAAAAGTGCCACCTGA
    AAGGCATGTTCAGCTTCTCAGAGCAGTTGCAGTACTTTTTGGTTATGTAAACTCAATGGCTAGGATTCCT
    CAAAGCCATTCCAGCTAAGATTCATACCTCAGAGCCCACCAAAGTGGCAAATCATAAATAGGTTAAAGCA
    TCTCCCCACTTTCAATGCAAGGTATTTTGGTCCTGTTTGGTAGAAAGAAAAGAACACAGGAGGGGAGATT
    GGGAGCCCACACTCGAATTCTGGTTCTGCCAAACCAGCCTTGTGATCTTGGGTAAATTCCCTACCACCTC
    TGGACTCCATCAGTAAAATTGGGCGTGGACTAGGTGATCTCATAGATCCTTCCTGCTGGAACATTCTATG
    GCTTGAATTATATTCTCCTAATTATTGTCAAAATTGCTGTTATTAAGTATCTACTGTGTGCCAGGCACTT
    TAAATAAATATTGTGTCTAATCTTCAAAACAAATTTGCAAGGAAGGTTTTTGGAGATAAGGAAACTGAGA
    CTCAGGATTAAGTAACACACCTAAAGTCACAGGTGAGCTTGGAACTGAACCCAAGTGTGCCCCCACTCCA
    CTGGAATTTGCTTGCCAGGATGCCAATGAGTTGTAGCTTCATTTTTCTTAGAGACTTTCCTGGCTGTGGT
    TGAACAATGAAAAGGCCCTCTAGTGGTGTTTGTTTTAGGGACACTTAGGTGATAACAATTCTGGTATTCT
    TTCCCAGACATGTAACAAGAGTAACATGTGTGAAAGCAGCAAAGAGGCACTGGCAGAAAACAACCTGAAC
    CTTCCAAAGATGGCTGAAAAAGATGGATGCTTCCAATCTGGATTCAATGAGGTACCAACTTGTCGCACTC
    ACTTTTCACTATTCCTTAGGCAAAACTTCTCCCTCTTGCATGCAGTGCCTGTATACATATAGATCCAGGC
    AGCAACAAAAAGTGGGTAAATGTAAAGAATGTTATGTAAATTTCATGAGGAGGCCAACTTCAAGCTTTTT
    TAAAGGCAGTTTATTCTTGGACAGGTATGGCCAGAGATGGTGCCACTGTGGTGAGATTTTAACAACTGTC
    AAATGTTTAAAACTCCCACAGGTTTAATTAGTTCATCCTGGGAAAGGTACTCTCAGGGCCTTTTCCCTCT
    CTGGCTGCCCCTGGCAGGGTCCAGGTCTGCCCTCCCTCCCTGCCCAGCTCATTCTCCACAGTGAGATAAC
    CTGCACTGTCTTCTGATTATTTTATAAAAGGAGGTTCCAGCCCAGCATTAACAAGGGCAAGAGTGCAGGA
    AGAACATCAAGGGGGACAATCAGAGAAGGATCCCCATTGCCACATTCTAGCATCTGTTGGGCTTTGGATA
    AAACTAATTACATGGGGCCTCTGATTGTCCAGTTATTTAAAATGGTGCTGTCCAATGTCCCAAAACATGC
    TGCCTAAGAGGTACTTGAAGTTCTCTAGAGGAGCAGAGGGAAAAGATGTCGAACTGTGGCAATTTTAACT
    TTTCAAATTGATTCTATCTCCTGGCGATAACCAATTTTCCCACCATCTTTCCTCTTAGGAGACTTGCCTG
    GTGAAAATCATCACTGGTCTTTTGGAGTTTGAGGTATACCTAGAGTACCTCCAGAACAGATTTGAGAGTA
    GTGAGGAACAAGCCAGAGCTGTGCAGATGAGTACAAAAGTCCTGATCCAGTTCCTGCAGAAAAAGGTGGG
    TGTGTCCTCATTCCCTCAACTTGGTGTGGGGGAAGACAGGCTCAAAGACAGTGTCCTGGACAACTCAGGG
    ATGCAATGCCACTTCCAAAAGAGAAGGCTACACGTAAACAAAAGAGTCTGAGAAATAGTTTCTGATTGTT
    ATTGTTAAATCTTTTTTTGTTTGTTTGGTTGGTTGGCTCTCTTCTGCAAAGGACATCAATAACTGTATTT
    TAAACTATATATTAACTGAGGTGGATTTTAACATCAATTTTTAATAGTGCAAGAGATTTAAAACCAAAGG
    CGGGGGGGCGGGCAGAAAAAAGTGCATCCAACTCCAGCCAGTGATCCACAGAAACAAAGACCAAGGAGCA
    CAAAATGATTTTAAGATTTTAGTCATTGCCAAGTGACATTCTTCTCACTGTGGTTGTTTCAATTCTTTTT
    CCTACCTTTTACCAGAGAGTTAGTTCAGAGAAATGGTCAGAGACTCAAGGGTGGAAAGAGGTACCAAAGG
    CTTTGGCCACCAGTAGCTGGCTATTCAGACAGCAGGGAGTAGACTTGCTGGCTAGCATGTGGAGGAGCCA
    AAGCTCAATAAGAAGGGGCCTAGAATGAAACCCTTGGTGCTGATCCTGCCTCTGCCATTTCTACTTAAGC
    CAGGGTTTCTCATATGTTAACATGCATGGGAATTCCCTGGGCATCTTCTTGTGGTGTGGAGTCTGACTTA
    GCAAGCCTCGGGTGGGTTTGAGGGTCAAATTTCTACCAGGCTTATATCCCTGGTGATGCTGCAGAATTCC
    AGGACCACACTTGGAGGTTTAAGGCCTTCCACAAGTTACTTATCCCATATGGTGGGTCTATGGAAAGGTG
    TTTCCCAGTCCTCTTTACACCACCGGATCAGTGGTCTTTCAACAGATCCTAAAGGGATGGTGAGAGGGAA
    ACTGGAGAAAAGTATCAGATTTAGAGGCCACTGAAGAACCCATATTAAAATGCCTTTAAGTATGGGCTCT
    TCATTCATATACTAAATATGAACTATGTGCCAGGCATTATTTCATATGACAGAATACAAACAAATAAGAT
    AGTGATGCTGGTCAGGCTTGGTGGCTCATGCCTGTATTCCCTAAACTTTGGGAGCCTAAGGTGAGAACTC
    CTTGAACTCCTAAGGCCAGGAGTTCAAGACCAGCCTGGATAACATAGCAAGACCCCATCTCTACAAAAAA
    CCAAAACCAAACAAACAAAAATGATAGTGGTGCTTCCCTCAGGATGCTTGTGGTCTAATGGGAGACAGAA
    CAGCAAAGGGATGATTAGAAGTTGGTTGCTGTGAGCCAGGCACAGTGCTGATATAATCCCAGCGCTATGG
    GAGGCTGAGGTGGGTGGATCATTTGAGGCCAGGAGTTTAAGACCAGCCTGGTCAACATGGTAAAACCCCA
    TCTCTACTTAAAAATACAAAAAAGTTAGCCAGGCATGGTGGCATACACCTGTAACCCAGCTACTCAGGAG
    GCTGAGGCACATGAATCACTTGAACCCAGGAGGCAGAGGTTGCTGTGCACCACTGCACTCCAGCCTGGGT
    GACAGAACGAGACCTTGACTCAAAAAAAAAAAAAAGAAGTTTGTTGCTATGGAAGGGTCCTACTCAGAGC
    AGGCACCCCAGTTAATCTCATTCACCCCACATTTCACATTTGAACATCATCCCATAGCCCAGAGCATCCC
    TCCACTGCAAAGGATTTATTCAACATTTAAACAATCCTTTTTACTTTCATTTTCCTTCAGGCAAAGAATC
    TAGATGCAATAACCACCCCTGACCCAACCACAAATGCCAGCCTGCTGACGAAGCTGCAGGCACAGAACCA
    GTGGCTGCAGGACATGACAACTCATCTCATTCTGCGCAGCTTTAAGGAGTTCCTGCAGTCCAGCCTGAGG
    GCTCTTCGGCAAATGTAGCATGGGCACCTCAGATTGTTGTTGTTAATGGGCATTCCTTCTTCTGGTCAGA
    AACCTGTCCACTGGGCACAGAACTTATGTTGTTCTCTATGGAGAACTAAAAGTATGAGCGTTAGGACACT
    ATTTTAATTATTTTTAATTTATTAATATTTAAATATGTGAAGCTGAGTTAATTTATGTAAGTCATATTTA
    TATTTTTAAGAAGTACCACTTGAAACATTTTATGTATTAGTTTTGAAATAATAATGGAAAGTGGCTATGC
    AGTTTGAATATCCTTTGTTTCAGAGCCAGATCATTTCTTGGAAAGTGTAGGCTTACCTCAAATAAATGGC
    TAACTTATACATATTTTTAAAGAAATATTTATATTGTATTTATATAATGTATAAATGGTTTTTATACCAA
    TAAATGGCATTTTAAAAAATTCAGCA
  • Protein Sequence : Show Sequence
    >gi|10834984|ref|NP_000591.1| interleukin-6 precursor [Homo sapiens]
    MNSFSTSAFGPVAFSLGLLLVLPAAFPAPVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKE
    TCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQA
    RAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALR
    QM
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
31. lipY
  • Gene Name : lipY
  • Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
  • NCBI Gene ID : 888677
  • NCBI Protein GI : 57117054
  • Locus Tag : Rv3097c
  • Genbank Accession : BX842581
  • Protein Accession : YP_177924
  • Taxonomy ID : 83332
  • Gene Starting Position : 3465777
  • Gene Ending Position : 3467090
  • Gene Strand (Orientation) : -
  • Protein Name : triacylglycerol lipase
  • Protein pI : 4.26
  • Protein Weight : 40955.98
  • Protein Length : 437
  • Protein Note : 3.1.1.3
  • DNA Sequence : Show Sequence
    >gi|57116681:3465777-3467090 Mycobacterium tuberculosis H37Rv chromosome, complete genome
    ATCAGGCGGCGATACCGAGTTGCTGGTTAATCTGCGGCCAGGACAGCAAACCCCAGGGGGTGAGCAGTAT
    CCAGTCGTGGATTTGCCAGGGGGCCAGTACGAAGCTGAACGGCGCTCCTTGGACTACGGCTGTGTGCTCG
    AGGACAACCGCTTGTTGTGCGAGCGGATCAAGCGAGCCCGAATAGACATACGTCGGCGGAAGACCGTTCA
    GCGACCCATACAGCGGACTGACCAGCGGGTCGTTGACCGCAAGATTGCCTGCCCACGCCTGGCTGATCTG
    CCAGGTCCCCACATCGAGCCACGGGGACAGCAACACCATGGACGACGGTACTGGGTTGCCCTGGCTCACC
    ATGTATTGGGCGGCCGCCAGTGCGAGGTTGCCGCCCGCGGAGTCCCCGACCACGCTGACGTTGGAGACCC
    CGTGTTGCGCGATTTGCGTGGAGATGAGCCCGGCCATCGCCGGTACTACCGTCCCGGCAGTGCCTCCTTC
    CTGCACCAACGGGTAAATCGGCACTTGCACGGTCGCGCCGGTCTGGTAAGCCGTCACCGAGTAGTTGAGC
    CAGTGGAAGATTGACGGCGGCAGGATAAACGCGCCGCCGTGAATGGCAACCACGTATTCGCCGGTTGGAT
    GAGCCGGCGTGATCTGCACGACGCTCATCCCGTCATAGGTGGTGTACTGGACCGTCTGTCCCAGCAGCGA
    GTTCAGCAACGGCGGTGGGGAGTTGCCAAGAAACCACGACAGCGGCGGTATGTCGCTGGCAATGAGCGCT
    AAAAGTGGATTGTTTGGGATTGCAAAGTGAGTTTCGAGCGCAGACAAACTCAGCAGGGGTTTCACCGGCC
    ACAGCGAAGCGATGTCGAATCCGGCAGCCCCTGACGGCGTGCCGGTGAAGATCCCTGCCTGCGCCGCCGC
    GAAAGGCGGAACCTGCGTGAATCCGGCGGCCAGCGCCGTGGGGGCCCGCTGAATTTCCTGGTGAATCGTG
    GCAAAACCGTTCCCGATACCGCTGGCGAATTCACTCTGCAACAGCGAAGCGTTGGCCAGCTCGGCGGCGG
    CATACCCCTTGGCCGCGCCTGTCAATGCCTGCACAAACCGCTCATGAAACACCGCAAGCTGTGCGCTAAG
    AGCTTGATAGTCCTGACCATGGCCGGAAAACAAAGCCGCGATCGCGGCTGACACCTCGTCCTCGGCAGCG
    GCTAATACCGTCGTGGTGGCACCCGCGACACCCTGGCTCGCCGTCGCGACCACCGAACCAATCGAAGCCA
    CGTCTGTGGCCGCGGCGGACATCACCTCCGGCAACGCAACAACATAAGACACCA
  • Protein Sequence : Show Sequence
    >gi|57117054|ref|YP_177924.1| lipY gene product [Mycobacterium tuberculosis H37Rv]
    MVSYVVALPEVMSAAATDVASIGSVVATASQGVAGATTTVLAAAEDEVSAAIAALFSGHGQDYQALSAQL
    AVFHERFVQALTGAAKGYAAAELANASLLQSEFASGIGNGFATIHQEIQRAPTALAAGFTQVPPFAAAQA
    GIFTGTPSGAAGFDIASLWPVKPLLSLSALETHFAIPNNPLLALIASDIPPLSWFLGNSPPPLLNSLLGQ
    TVQYTTYDGMSVVQITPAHPTGEYVVAIHGGAFILPPSIFHWLNYSVTAYQTGATVQVPIYPLVQEGGTA
    GTVVPAMAGLISTQIAQHGVSNVSVVGDSAGGNLALAAAQYMVSQGNPVPSSMVLLSPWLDVGTWQISQA
    WAGNLAVNDPLVSPLYGSLNGLPPTYVYSGSLDPLAQQAVVLEHTAVVQGAPFSFVLAPWQIHDWILLTP
    WGLLSWPQINQQLGIAA
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
32. Ltf
  • Gene Name : Ltf
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 17002
  • NCBI Protein GI : 31560677
  • Genbank Accession : AC118727
  • Protein Accession : NP_032548
  • Taxonomy ID : 10090
  • Chromosome No : 9
  • Gene Starting Position : 111019291
  • Gene Ending Position : 111042765
  • Gene Strand (Orientation) : +
  • Protein Name : lactotransferrin
  • Protein pI : 8.61
  • Protein Weight : 73470.01
  • Protein Length : 707
  • Protein Note : Also known as Lf; Csp82; Ms10r; MMS10R
  • DNA Sequence : Show Sequence
    >gi|372099101:111019291-111042765 Mus musculus strain C57BL/6J chromosome 9, GRCm38 C57BL/6J
    CAGGCTTGTCCTCTAGGTCTCCCAAGACCACAGACATGAGGCTGCTCATCCCTTCCTTGATATTTCTTGA
    GGCCCTTGGTAAGTGCAGGTGCCCAGAGGTAGGGAGGGGCGGGAGGTCACCTGCTGGGAAGCTGGGCACT
    AAATGCACTCTTCTGTTATCTAGGGAAGACTGTTGGGCTCAGAGAGGGTGAGATGTTCCTGTGAGTCCCA
    GTTCACTGCCTCCCAAGAAGGCTTCTTTGTACCAACATTGGGGACCCTCCATGTTTGACTCTATGCCCTC
    TCTTAGTCCTCCTGCTGCTGGACTCCACATCTAACCCAGGCATCCTATACAGGCAGGCTAGCTCAGTGGC
    CAGTGGTGCTGGTGCCCAACCGCCTAGGTTCAAATTCTAGAGTCAACACAACTTAGCTGGAAAGTCATAT
    AACTTGGTAAAGCTACACAATGCCTAGAACTATGGGGGTATTGACAAAAATCACACTGAAGGATAGATGG
    GGTGACCCAGTGAGTAAAGGTGCTTGCTACCAAGACTGAAGACAGAAGTCCTAGTCCCAGAACTCACTCA
    ATGGAACTACCAGCTTTCTATCTATAAATTTATCTTTTCTGGTCGAGTGTGGTGGTGCACACCTTTGATT
    CTAGCACTTGGGAAGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGC
    AGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAG
    GCAGAGGCAGGCAGATCTCTGTGAGTTCAAGGTCAGCCTGCTCTACATAGTGAGTTCCAGGATAGCCAAG
    GCTACGTAGAGACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTGGAGTTATCCAATATTTTGTCTT
    GGATTTGGCTTCCTTCATCTAGTCATGTTATATGTATGTGTATTATTTTTTATATAATTGAAGAATATTC
    CATTGAATGGATGTACTGCATTTTCTTCAGCCATTCCTATTCACCAGTGTTTGGGATTTATGTAGTTAGT
    TGGGTTTTTTTGTTGTTGTTGTTATTTTGAGACCTTTTTCCTATCTAAATAATGCTACTATGAATATTAA
    TGCTAGATCTTTAAGCAGATTTATGTTTTTATTTCTATGAGCAATTGCCAACCTGTTTCCCAAAGTGGCT
    ACATCATTTTACAGTCTCAGCAATGTAAGAAGGTTTCCATGTTTGCAACATTAAGATCCAGTGTGCACAT
    GAGTAAAACATGAGCCACGCCATTTCCCTGCTTCCCTGCCAGGTTCATCTAGGACAAGGCTGATGGAGGT
    CAATAAAATGATCTTTGGAGTGGATCATCTTTGGGGGTGGGGTGGGTGTCTGAGTCTCATCCCTCTGTGG
    TCCAGCAGCTTAGCTTAACTAGAGTACAGTGGCTGAACAAGACCACGTAACACGGAGGGTGGGGCAGTGA
    CCACGGGCAGTCTACAGAATGCCCTCAGACATCGGGGAGGTCTTCCCCAACGAGCACTGCTGGATTCCAT
    TCTGTCTTTCTCCCAGGACTCTGTCTAGCTAAGGCAACAACTGTTCAATGGTGTGCTGTGTCAAATTCTG
    AGGAAGAAAAATGTTTAAGGTGGCAGAACGAGATGAGAAAAGTGGGTGGCCCGCCGCTCAGTTGTGTCAA
    GAAATCCTCCACCCGCCAGTGCATCCAGGCCATTGTGGTAAGCTAACACTCCAACCCGAGTGGAGGAAAG
    CAAGGAGAGGTGCGCAGTCCAACCACAAGTTCGAGTCTCTGGTCCCTCTGCTTCACTTCCTGGGCACCAG
    TCAGTTGAGAGCAGTCTTTGCTCACAGAAACCGGTGTCTCTTGATATCTAAAGAAAGACAAATGACAGGC
    CTCTTATGGGGTAACTGGGGTCTTATTTCAAATTGTTCATAGGAACTATTTTTTTAAAAGCCTAAGCAGC
    CATATTTGATGAATATGTAATGTCTCTAATCCCTTGAGAAATAAGCCTGGTGCAGCCCTAGATGCCATAT
    CCCAACTGTTGGGTAGGGATGGCTAGGACTCCAGACATTAAAAAGACACTTGTTTGCCCTCCAGCACCTA
    TGTTAAAACCTAGAGATAGTAGCTTTCCCACTTGGACTCTGGGTCTAGGAAGTAGAGACTGGAGGTCTAA
    ACAAACTGGTGAGCTCCAGGTTTGGCAAAAGACTTTGTCTCAAAACGTAAGTCAGAAAGAGATGGAGGAA
    GACACCAGAAGTCGAGCTCTGGCCTCCACACGTTCATGTCCTCCAGTGTGTGAGCACACACACACACACA
    CACACACACACACTGCGCACATCTACAAGACTGAAAATACACATGAAGGCAAAATAGGTGATAAAGTATG
    TGGATCAAAAAGCTTTAAATACAGAAGATTGTGACAGTGTGACCAAGAGTAACTGAAGTGGACAGGCATA
    CGTAGGGGTCACACACAACTTCAGGAGTTAAAGCCCTGGGGGTGCTTACTGCTCACATCGAATGACCCCT
    GACCTGTGCTGTTGTCATCTTCTAGGTAGGACATATGGACAATACGAAGGGCTAACTCATTCTGAAGTCC
    TGAAATCTTTTCTCCTGAAGCATGTCTGGACCCATTTTCTTTCAGACAAACAGAGCTGATGCCATGACTC
    TTGATGGTGGCACTATGTTCGATGCAGGAAAGCCCCCCTACAAACTGCGACCTGTGGCAGCTGAAGTCTA
    CGGGACCAAAGAGCGTGAGTTCTTTTTCTGTGGACGCTGAAACCTCGTGACTGTGTTGTGGCTATGAAAT
    GGTTTCTGTGGCGTGGGAGTGGACGAGTAAACCTAGTCAGTGCCTCTCACTGGGGAACAGAGTCTCTGGA
    CTCTGGATGACAGGTCCTCCTGGTGCTTGGGTCTCCTTGCCATGGCAGCTTGGGCAGAGTGGATTCTTCA
    CCTCCCTCTCATCGGGGCTAAGCCTGTCTAGTGCCTCTGTTCCTCTGCAGAGCCCCGGACTCACTACTAT
    GCGGTAGCAGTCGTGAAGAACAGCAGTAACTTTCATCTGAACCAACTCCAAGGCCTGAGGTCCTGCCACA
    CCGGCATTGGCAGGAGTGCGGGGTGGAAAATCCCTATAGGGACACTTCGTCCATACCTGAATTGGAATGG
    GCCACCTGCATCCCTTGAGGAAGGTAAAATGGCTTGTGGGGGAGCGGGGAGAGATCCTGAGTGGTCCAAG
    CTGGGAATTTCTAGTCTCACTGTAGACACAAGCCACTTTGGGATGGCTTTCCATATGGGATGAGTCCAAG
    GTTGGGCAGAGGGAGTCTAATCAAGTAGAAGCAGGGGCCATCACACCAACAACTCCGGGAGCCGCCAGTA
    GCTGTTCTCTGGGCAAAGATGCACGTCTGAGTTCCGAAGCCCACTCAGCTCTGTCCTGTTCTGCCTGGCG
    GAGGAGGGGGCTCTTGGTCCGTGGAGGGTGGAGGGGTGGCTGCTCCTGGGAGACAGGACTGCAGTGGCTG
    GGATCGGCTCATCCTCTGTGGCCCACTTCTCTGTGTTTGACAGCGGTATCCAAGTTCTTCTCAAAGAGCT
    GTGTTCCCGGTGCCCAAAAGGATAGATTCCCCAACCTGTGTAGCTCGTGTGCAGGGACAGGAGCCAACAA
    ATGTGCCTCTTCCCCCGAGGAGCCATACTCAGGTTATGCTGGAGCCTTGAGGTGAGCGACACCATTCTCT
    CCTCCCCCACGGCCCATGGTCGCACAGCTTCAGGCCAAGGTGCGGGATGGAGATTTGGTCCTACTGATGT
    GCAGACGGAGAACCCAAGCCTAAGAAGTGGTTGGTCTCTCTACTACATGTCCTTCTCCAGCCCCTTAGAG
    GCCCAACAAGGCCCCAAAGTCTATATCACTTTGATGGAAGCTAACCTGGTTATGGCCCCTCTGATCACCT
    CACGCCTGATTTTGCTCTCCTATTTTTTGGGGTTATCCTGCTAACTTTCTCCCTGTTTTCATCCCATCTG
    GGTGGGGCAGCCATTGTGGCCTTGATATTTAGTCCAACTCAGGCCTCTCTCTCGAGTAGGAGAGGGTGAC
    CTCTGTCTGACTTCCTCCTCTGGCCTCTATGCTGTTGCCTAGGCTCAGCACCTCCTCTCTAGTCCTTTCC
    TGAAGCTCAGGCACTTTTGTAGAGTGACTTCCCTAGGTTCTTGCTGGCCCTAGCACATTTGCCCACTCAG
    CATGGAGAGAAAGAGCCCCATGGTCGGGGACACCTCATCTTTAACTGCGTTCTTCTCATTTCACAGGTGT
    CTGAGAGACAATGCTGGAGATGTGGCTTTTACCAGAGGAAGCACGGTATTTGGTAAGGGCAAGGGTCAGG
    CTGTGGGTGTGGCCACCTCTTAAGTGCTTTATCTGTGATGTGCGGGTCACCAGATTCCCCAGTTGTGATC
    AAGTTGCCTACTGGGTCCAACTCTTCTTTTAGGTTTTTCTTTTAAAAAAATACTTTAAGATTAATTATGA
    TTTTACGTGCATGGGTGTTTTGCCTTCGTGTATGTCTGTGCACCACATGTGTGCCTGATACTCAAGGAGA
    CCAGAAGAGGGTGTCAGATCCCTTGGGACTGGAGCTACAGGTGGTTGTGAGCTGCTATGTGGGTGCTGGG
    AAGTGAACCCAGGTTTTCTGTGAGAGTAACCACTGAGCAACCTCTCCAGCCCCTACCTCATGCTCTGAAA
    TCTCCCACTTCTACCCCCTTCTCCTTCCCCTCACAGACACTCTCACCAGGGAGTTCGTCATGTGCTTGCC
    CAGGTGTACACGCCTCTGCACCATAAACTTCACTGGCTTGTGTCTCTGGATTCTGAGTTCACACAGCTTT
    TACTGAACACTAGAAGCAGCGCGAGCCCAGGGCTTTCCACTCAGCATTGGGGGTCCAAGATTGATCCGTG
    GTTTTACTACACGAGTAACTCCGTGGTTCTGACAGTAGAAGAACGATCCGTGGAACATGTCTACCATATT
    TAATTAACGTCTCTGTCAGTCTAATGACAGACAGACAGGCGCGGCGGTCCTCCCTCCTTTCGGCTTTACC
    TTTTTGAGTGCTCAGGAAGAGTGCTCATCTTCACACGTTTCCCTTCCTCCCAGAGGAGTTACCAAATAAA
    GCCGAAAGGGACCAGTACAAGCTGCTCTGCCCAGACAACACCTGGAAGCCGGTGACAGAATACAAGGAGT
    GCCACCTGGCCCAAGTCCCTTCACATGCTGTCGTATCCCGAAGCACGAATGACAAAGAAGAGGCCATCTG
    GGAGCTTCTCCGCCAGTCACAGGTACAGCTACAAGTGCACAGCACCCCTTGCCTGCTTAGGTTTAGGGGT
    GGGGAGGGCCTCTCTTTCCCTAACTTCAGCAGTTGAGAGAGTTCCATAGTTTAAGGACCATGGGCCCTGA
    CTCTCCCTTGGACAGTAGAGACATAGGAACCTTCTTCAGTGTCAGCCCCTAGCAGGCTTTCCTGGGCTTC
    CGCAGAGGCAGGGGTGGGTACCGGATGCTATTCTTAGGTCCCCTAAGGACCTTGGGACAAAACAGTTTTC
    TGATGAGGTAAGTTATCGTTGAGCTCATGTCTCTGCAATGAGCAGAGTCCCTGGGAGCTCGGATATCAAT
    TCCATTTGAATTGTCCCCAGATCCCAAGTAGCTGTTTCCCACACTGGGTTTCGATTCCTTCCTGCCTACT
    GCTGGTGACCATAATTCTATTTTCTTTCCTTAAACAGGAGAAGTTTGGAAAAAAACAAGCATCGGGATTC
    CAGCTCTTTGCCTCCCCCTCGGGACAGAAGGACCTGCTGTTCAAGGAGTCTGCCATTGGCTTTGTGAGGG
    TTCCCCAGAAGGTAGATGTAGGGCTCTACCTGACCTTCAGCTACACCACATCCATACAGAACCTGAATAA
    AAGTAAGTGGACCCCAGGCAGGAACCCAAGGGTACTGAGCCAGCAGTACCTCTGGGTGGGTGACCTCTGC
    TCTGGGAGGGTCTCTCCGCTACTGGTGGGGCTGACGCACATACTGTGTGTCAAGTAAGTCAAGGCCATTG
    TGAAAAATGTCTCCAAAAAAAGTCCCAGACGCTGGACTAATGCTTCCATATTCCTCTGTGTATGCCAGGA
    TCCCAAGGCCTCTTATTAAGTGAGAAAGAGAGGCATGGACTAAGAAAAGAAGAATCTGGGGAGATTACTC
    CAGAGGCTAGGTGAAGCCCCCCTTCTGTGCTGGGATTCGAACTTGGCTGAAGAGATTACAATGGGCTGTA
    GAGATGACAACTTTGGCTATGTTTATCCTACTTGGGAGGACTTAGAGAGGGAGTCTGAGTGAACCAGGAG
    ACTGTCCTGTGACATCTGCTGGTGACAGAGCCTTATGGGTGGGTGGTCGGGCTCAGTCAAGGTCCTCTAA
    GCCAGCCTTTGGGGTATACACTAGTTTCCATGTCATGAAGCTTTTATTCTGAGGGACAGAACATTAGGCA
    CTCCACTCATTACACCAAGGACTGATGGATGAATTAGAAGCCAGGGAAGCCCTCTGAAAGAGCAGAAGGA
    GAAACAGAAAGCTTCCGGCCAAATCCAGTTTGAGGGCTGTGATGCGGGGAAAGAGAAGGTCTCACACAGA
    TTCCTCGGTCTCAGAAGATCTGGGCAGGACCTTGATCCCCAAGGAGGGCAGAGGTGGGAGTGAGGCAGCG
    CCTGAGTGTTCCTCACCACTGTCCTTCCCCACAGAGCAGCAGGATGTGATAGCCTCAAAGGCCCGGGTCA
    CATGGTGTGCCGTGGGCAGTGAGGAGAAGCGCAAGTGTGATCAGTGGAACAGAGCAAGCAGAGGCAGGGT
    CACCTGCATCTCATTCCCCACCACGGAAGACTGCATTGTCGCAATCATGGTAGGGAGCCCGGGTCACACG
    TGCTGTGGGTGGTGGGCTTCTTAGAGGGCACGGACCATGGTCATCTACTTATAGTGAGGTTGGTCACCCG
    AGAGGCCACTGTGAAGAAGGAACACCCAAAAGAGAAGGTGGGCTCGGGACCACTCATGCACATGTGTTGC
    ATGAAGGAGCGGCCTTCTAGGTTTGAAAACCAATTTGTTGTGGAATTTCTGCCCTTTCCAGTAAAAGTAC
    AATTTAGGGTTATTAGGTTCATTTGGAAAATATGGAACAATTTTAGCATCTCTCTCTCTCTCTCTCTCTC
    TCTCTCTCTCTCTCACACACACACACACACACACACACACACACACACACACACACCTCTGCATGCAACT
    TCATATAGAAATATTTACAGTGAGAGTTTTGAAATTCCCCACCCCAGGTTTCTCTGTCCATGTAGGCCGA
    GCAATTCTGGATTTGTATGGGTTATTCTCTTTTGATCCCTCGTTCAAATAAGCAGTATACCATGGCTACT
    TCCCCACATCAATGCTAATCTCGGGAAACAGTTTTAAATGCTTATTTTATTTTATGTATATGTGTGAGTG
    TCTGAACGTGTATGGGAGCCACATGCATGCAGGTGCCAGAAGAGGGTGTTGGATCCTCCAGAAGTAGCGT
    TAGAGACAGTTGGAGCTGCCATGTTGGTGCTGGAACTGAATTCAGGTTCCCCGAAAGAGCAATGGACGCT
    CTTAGCCACTGAACCAAATCTCTTGTCCCCTCTCATCATTTTTAACGCCCTTGTATAGCAGTGCTGACTG
    GGTAAAGGAGCGTGCAGTCCTGATAAACTCGTGTTTCTGCGAGGGTTGTGGCTTGTTCATTCTCATGAGT
    CAACTTGATACCCAATCTCTAGGTACCACGTTGAGTATCGTGCTAAGAAGCAGAAGTTAGCTGAAAGCAG
    CTTTTCTCAGCGTGTTTTCTGTGAGTGAGGCTGGTTCAGAAAGGAGTGCCAGGAGTGCTCGGGTACAGGA
    AGAGTTCTGTTCAGAAGACAGAGTTAGTGTCTCAGGTGTGTGGGTGACAGCCCTAAGATAGTGAGACCGG
    CGGAGGACTGAGTCCATGTGGTATTTCCTGGGGGTGTTACGTGCAAACAACACGTGTCTTGAGGCCATCC
    CTGAGAGCTCCGAAGACAGCATCTTTGTGTGGTGTGTGTGACTGGCTCTGTCTCTGCAGAAGGGAGATGC
    TGATGCCATGAGCCTGGATGGAGGCTATATCTACACTGCGGGCAAGTGCGGTTTAGTTCCAGTCTTGGCA
    GAGAACCAGAGTAAGTGGAGTTGGTGACCACAGGCTATGCCATATGAAGTTGACTTGGGAGTAGAGAGGT
    GTTACCTCACTCACTAGATCTTGGAGAAGATGGAGACAGAATCAGAAGAAGGAATAAATGAAAGGGGCCA
    TGACACCCACTTCACCCCATTCCCTTAGATTGACAGATCATGATAGTTTACTTCTTGATTAAAACAAAAA
    CAAAGACATCTTTGGGAATGGTGAGATGGCTCAGCTGGTACAGGTGACAACGGCTGCCAAGCCTGTTGAC
    CTAAGTTTGATGCCTAAGACCCACATGGTGGAAGGAACCAACTCCCACAAACTGTATGCAGCCCTCTATG
    CATGCACACACAGGTCATGTGCACCTGACATTATGTATATATAACATATTATTATAAATATGTATTTATT
    AACATATATGTTGCACACACAGAGCATGCATGTGCTGACAACAGGTGCATATTGACAACATGCATGTGCT
    ACTAGCATGAATACACTGATAGCATAAATGTACTGGCACCCAGATGAAGGGTCAGCCATCCCCAGAAGTC
    ACCCTGTGCCCTGCTGTCACCATACTTCCAAAAGGCAGCGACTCTCCTGACTTCCAAAGACATAGATCAC
    CTTGCCTCTCCTTTAAGTTAATGGAAAAAGAATTGAGCATTTGATGTCTGCTGTGCCTTGCTCAGCATTA
    TTTTTGTGACATCCACCCACACAAACGTGTGGCTGCTGATTAGCCAGTCTCGTGGCTGCATAACGTTCTA
    TTGTGTGACTGGGTGGCCGTGCTTCTCCATTGTCTTCATGGATGGGTGTTTCAGTGATTTCCATCTGGTG
    GTTATTGTGAAAGATACGGCTACAGCCATCCACGACCACGTCTATGATGAAAATGTGTATTTATTTCTGC
    TGCATTGATATACCGAGGCGTTCCCTTTGAGTTTGATCTTATCGTATTGTGGGCATTTGAAAGTTTATGG
    CCTCTCAGGAAATCTGAGTTGTCAGTAACGCTTTGCTTGTCTTTGCCCCTTCAGAATCCTCCAAAAGCAA
    TGGCTTGGATTGTGTGAACAGACCAGTGGAAGGTAAGTCGGGGGCAAAAATAAGTCACCACTCGAGAGGA
    GGCAGGGGGAAGGTCACTAACCACACAGAAGCCAAGCCTGCTGTTATTACAGATACAAATGGTTGATGGA
    GGAATAATTACAACTAAAGTCTGAATGCATCCTCATGCTTGCTGGGGAACTTTCATGTGGTCACTCTTAA
    TGCAAGCTTCTGTGGAAAGAGAAATGTTGCTCCCCCATTCTGTACATTCGAGGGAACAGTGGCCCAGAGA
    GTCCCAGTGTCTCCGATCTCACTCATACTGGCACTACGCTGCTGCAGATGCTGGATTGGAACCAGGGCTT
    CTCGCCTCCGCAAGCCCACGGCTCTGCACTAGCCTCAGCTGCTCTCCCGAGTTGGAGCTTGCTGAGCTGT
    GGAAGCCTTGGTACTTGCCCTCAGTTGGGTTATCAGATGCCTTCGGAGGGGTTCAGCAACCATGTTAACT
    CCCTCACTGTGCTGGGCTGGTTGCACTCAGCTCTTGGCGGTCCTTGGGTGCATGCCCTTAGCATTCATGA
    AGCAGATCTACTGGCCACCACAGCACTTACCTGTGTGTGTGATTTGTGCATTGCTGAGACCAATCTCATT
    GTTCAGAGTCTGTTGCCACTGGGTAAAGGTTTATAACTCAGCAATACTATGAGGCTGGTTGAGGACAGTT
    GCCTGTAACCACTTAGAGGACCAATTTTTCATATTATTTTATTTAACAATACAGGTTGGTCTTCCCTTGA
    CACAGAACACCTTTTTAAAATTTTCTTTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC
    CTTCCTTCCTTCCTTCCTTTCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCT
    TCCTTCCTTCCTTCCTTCCTTCTTTCCTTCCTTTTCCTAACAATGGCCAATGATCCATGGTCATGTAATC
    CTGTTGTTTTTCCGGCCTGGGTTGGAGACACTGTACTTGAATCCATGTGCACCCTAGAAGCTAAGATGTT
    CTCTCTGTCATCCGTTGCACAAGGGTACCTTGCTGTAGCAGCAGTTAGAAGAGAAGATGCTGGCTTCACC
    TGGAGCTCTTTGAGAGGCAAGAAGTCCTGCCACACTGCCGTGGACAGGACCGCAGGCTGGAACATCCCCA
    TGGGCCTGCTTGCTAACCAGACCAGATCCTGCAAATTTAGTAAGCATATGGGAGGTTCTCACGGGGTGGT
    CATCCTGGGTCTGGGAGTGAGTACTTGAGGCTGTGGAACCTTAGGGGAGGGAGAGGGCAAGACGGTTGCT
    GACTGGCTGTCGCTTGTGGGCCTCTTTTAGAATAATTAGGTTCTCCTTCTGGTCGCCTCATGGAACCATG
    GGTGCACAGGATGTTTCACACAGAACACTTCCTAAGCTTCCCCAATTCCACCCCACCCACTGGGAGATCC
    AGATCGCAATAGCATTTAATTAAAACATTAAGATATGCTCAACCTTATGAGTTAGCAGTTAGCTGAATTG
    TTAACAATCAGATGTTTAGGTTTTTTGTTTTGTTTTGTTTTGGTTTGGTTTGTTTTTTTCTCATCGTAAA
    TCTAGTAAGATAGCGTGAATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTATATG
    TAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGAGGGAGTCAGATCTCATCAGATCTCATTACAGAT
    GGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACCTTCGGAAGAGCAGTCAGGTGCTCTTA
    CCCACTGAGCCATCTCACCAGCCCTAGCTTGAATTTCTAATGTAGGCAAGAATGCAGTAAAATGGGCATT
    TTCAAATGTTTTCATGTGTATAAAAAACTGATACCTGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACT
    GAACTGCTCTTCCAGAGGTTCTGAGTTCAATTCCCAGCAACCACATGGTGGTGGCTCATGACTATCTGAT
    GGAATCTAATATCCTCTTTGGTATGTCTGAAGAGAGTGACAGTGTACTCACATACATAAAATAAATCTTT
    TTTAAAAAAAAAAAAACAAACCCTGAGGCCTTTTAAGAATGTTTCCAGAGAGTTGGCACCCAGCACTCCT
    GAACACAAATCATTAGACCTACCTAGTACCATGGGTGTACCAGTCACAGGAACATAACAGCCATCAGGCA
    CCTTAATTGAGAAGCCTGGGTTTCTATTTCTGATTTTAAAAAATAAAAAGAAGTCAAGTTGTGGAGCAAG
    AGGCCTATCTGTGCAGGTGTGGCCTTCTCAGACACCAGCCCTGATATCCTATCCATTTGTACAGTCTGAA
    GCCTTTCTGATTGCCAGTGGGTCTTTGGTTGGTGGTAGAATGTCATTCTGGGGTGACAGGTATCCTGACT
    GTGCTGTCTACGTACTGAGCTGACATCATGCTATGCCATCTGCTGGGTAAAGACTTTGTGTCGACTGTGG
    TCCGAAGCACTAACTTATTGTCTTTCCTCTGTCTAGATGAGTTCTTTAGCCAAAGCTGTGCCCCTGGTGC
    TGACCCCAAATCCAATCTCTGTGCCCTGTGTATTGGTGATGAGAAGGGTGAGAACAAGTGTGCTCCCAAC
    AGCAAAGAGAGATACCAAGGCTACACTGGGGCTTTAAGGTAAGTCTGGGGCAGAGTGCCACCAGCCCAGG
    GCCTCAGCCCCCATTCTCAACAAGCGGCACTGTAGTTGGGGTGGGGTGCTCTCATAGAAGAGTCAAGGAT
    CAATGGGGGCAGAAGAAGCAAGAGCCTCCTATCATACCCAGCACCCCAGAGAGTAGAGGTTGGACAGCGG
    GTCATGGGGCTTACAGGGCTGCAAGGCTCAGCTTTGTCACCCTCAAAGTCAACGGGTAGAGAGTGTTTCA
    GTGCATTTCAGAGGATTCTAGTGACCACCACCCTTCCTTCTCCCTCACTCAGATCACCATTATCCTATGT
    TTTTAGTTTTCTCCTTGATTTTGGCCTTGAAGGGGCTGGAGAGATGATTCAGCCGCTGAGTGCTTGCTGT
    TTCATACAGAACTGCGTTCAGTTTCCAGGATCCCAATCAGGTCCAGGGGATTCAGTGTCCTCCTCTTAGG
    TCCAGAGTACCCACATGTGCACATATGTACATACAGGTGCACACATAAATAATAATAGGTCTTTTTAAAA
    AGAAGTGGCAAAGAGAAAGCTACATTACCACACCTTCTCAGTGCTGCATGGGTCACAATGACTGTACAGC
    TCACATTTACGTGGGTGTTGATTCTCACGGCCCTGTGTAGAACATGAGGCTCACGCAGAGGCTCCTTCCT
    CCATCCTTCCTTTAACACTAGATGTTGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT
    TCCAAAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCTGTAATGAGATCTGATG
    CCCTCTTCTGGGGTGTCTGAAGACAGCTACAGTGTACTTACATATAATAAATAAACAAGTTAAAAAAAAA
    CACTAGATGTTGTCACAGACCCTCAGATGACAATATTCAAACGGGACTTTGGGCTTTTAAATATAAATTC
    AATAATTTTGCTCCCGTGAAACAATGACTTTTTTTTGGCTATATCTAAATGATTTGTAAACACTGATTTT
    TTTACGTGTATGAAACATGTCCTTAAGGACTCACATAGCCGGGCATTGGTGGCGCACGCCTTTAATCCCA
    GCACTAGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAAAGTGAGTTTCAGG
    ACAGCCAGGGCTACACAGAGAAACCCTGTCTCAAAACAAACAAACAAACAAACAAACAAACAAACAAAAA
    GATTTACATATAGAAAGCCTACAGCATGATAGCATGATTCACTCTTATTGATTGATTGATTCACTCTTTA
    AATAGATAAGTTTGATAAGAAGAACCCAAGGGAATCCTGGGATCTGTTCCCCCAGACTTGCAGCCTGGTA
    TTTTCTCTCTGGAAACATCTGCACACCCTTACTCCTGTATACACTAAAAGTTTTTCTTCTGCTCAATTAG
    ATCTAGAATCTAGGCTGGGGACTATACAACAAAGTAATCCAAAGCTGGAGCCAGGACTCTTGCTTTTCTC
    TTACATCTTTATCTAAAACTCATTTAGGTTAGCATATAGCATGCTGGGTTTCATCATGGTGTCTCTAAAT
    TCACGTGACATTATGTTTGGTTCCCATCTGCTCCCCTGTACTCTGTCACTTGCTTCTTTCCTCCCCAGCT
    CATCATTATTACATGCGTTCTTTACTCTCTCTAGTTCCCTGATTCCTTAAGATCTCTCCCTTCCAGGGAT
    GGAGAGATGGCTCAGCAGTTAAGAGCCCTGGCTTCTCTTGCAGAGGACCCAGGCTTGGTTCTCAGCATCT
    ACATGGTGATGCAAAAGCATTCCTCTGGCCTCAGCAGGCACCAGGCATTCAGCACACAAACATTAAGGAA
    ACACTCATACACATAAAATAAAAATAAATAAATACTTTCAAAATGTTCTCCTCCTTCTCTCTCATAGTCC
    TCGTTCTGGTTTCCTCACACACATGCATATACACACATACACACATATATACACATATACACATATACAC
    ACACATATACACACATATACACATACATACACAACACACACATACACTCACAACACACACATACACTCAC
    ATATACACATACATACACAAATGTATATATACACACACATACACACATATACACACATGCACACACATAC
    ACACACAAACAAACATATACTTTTACATTATGGCCCCACATGTGAAAGAAAACATGTACTATTTGTCTGA
    GTTTGGCTTATGAACATAATAATCTCCAGCTTCACACACTTTCTATAAACATAATGATTTCCTTTTTCTT
    TAAACTCCCATTGTGAATTCGTAGCACATTTTCTCTACCCATTCATCTGTTGATGGACATCGTGGCTGCT
    TCCACTTCTTGACTATGATGAATAGAGCTTCTATAGACACGGATATGCAAATATCTCTGTGGTAGTACAC
    AGAGTTCTTTGGGCCTATACCCAGGTGTGGTATAGCTGGGCCGGATGATAGTTTCAGTCTCAGGTTCTTA
    AGAAACGTCCACAGAGGCTGCACACGTTTCATTCTTGTATTAATAACTAGCATGCATATACTATTGGTTC
    TTACCTTTTTTAAGTTTCACGTATTTTTCTGAGTTCCTGTTATGCTACAAAACCTAGCCTCTCACACTGT
    CCCTGTCCCTCAATAACTCAACCACAAACTTGGCTTTTTCTGACACCTGGATTCATCTTATCGATGCTTG
    TGTTGCAATGACTACTCCAGCATCATTCTCATACTGAGGATGTGGCACAAGAGCGTGTGTCCCTAGCCAG
    GCATGGAGGTGCAGAGAAGTAGAACTTACTTCCTCAGGCTCTGAGGCAAGAGCTTCTACACGCTGAGCCA
    TCTCCTCAGCCCTGTTTTGATCCCTAGTGTTCTGAGGCTTCATTTCTCTATGTGAGTCCTCTCTGCTTTA
    TTAGAGTGGGCAGTTAGCAGACATGGATGGCTACAAATTATTCAGTTTGGTGAAATTATTATTTTGTTGA
    TACTTTTTATGCTCTTGTGTTTTAAACTCCAGACTCTTGTGGGCCGAGATCTGAGTTCCTAATATCCATT
    AGTCATATTTTCACTTCTAGTTTCTGTAAAATTTGCTTGGTTGAATCTTTTAATCCTGTGTCATTTTAAA
    GCATTTTTATGGTGAAGATTATTTTTATTTTCCTTTTAAAAATATATATATATTTATTTATTTATTTATT
    TTAAACATGTTTGGCTTGCATGTGTGTCTGCATACTACCTACATGCCTGGTGCCCTCAGAGTCAAGAAGA
    GGGAGTCAGAGCCTCTGGAACTGGAGGGATGGATTGTTGAGAGCCTCCATGTGGGTGCTGGGAATAGAAC
    CTAGGTCCTCTGGAAGAGCAGCCAGTGCTCTTAACCATTGATCCATCTCTCCAGCACCTATTTATTTTCT
    TTTGAATCTTCCTAACAATTCTTTGTATTTCCATTATTCCTTTTTTTAAAATCTTCTTTTATGTGCTTCA
    TGAATGTTTCTCATTTCTTTCTCATCTTTAATGATATTAAGTGTAATCCTCTCCCTTCTATCGTCTCTGA
    ATCTTGGGAGTACAATGCTTTTGGTATCCCTTTTCATGCTCTCCCTTCCGGCATCTGACAGCCTTTGCCC
    GTCTGACAGTGAGGTGGGTGAGTGCCACAGCTGACTCTCAGCTCTCCATCAGTTTTCCAGTGTGAGGTGC
    TGGGCCCCAGTTCAGGGCTCTTGGTTTGTGCCATTTGTCATGAGCATCCTAATAGCAGAGTCTACGGATG
    TTTTCTTGGACTCATCAGACTCTCTTGACGAAGAGTCTCACTGCCCACATCTTGGGAGGGATAGCCCTAA
    TGGCCAGCCTGGGTGGTGCGTAGGGATTGACTCCATTTTCTAGAACACAGACTTTTCAGTTTACTCCTTT
    ATGGATGGGGGTTGGAGGCGGGGAATGTTGAGACAGATCTAACCCTGTAGCCCAGGCTTGCTTCACACTC
    ACAGCAATCCACTTGTCTCAGCTTCCAGAGTCCTGGGATGACTGGCATGAGTCGCTGTACCCAGCTCCCT
    CTCCGTCTTCTCGGTTGTGTTTTCCTATACTTCCATTGGGTTTGTTTCCCTATACTGTGTGTGTTAAGGG
    TCAACCTCCCAGATGTTGGGATGAGAGTTGCTGCATGGGATGGGAGCATTAAACTCTGTTTCCAGCCACT
    CTCAGCGATGCCTTATATTTCCTGTTGCTGAGCCTTTCTGGCATCCCATGGTTGCACATTAACTTTCGTC
    AGAGCTGCTACCACCCCTGCCTTATGTTACAGCCTTCCTCCTTCAGTTAGGCCAGGACTGCTCAGCCATT
    TTCTTTCCAGATTCCAAAGGGTGTCTTCATTACCTTCTCTTTCTCTTTTATATGTTTATGGGTTAAAAAA
    AAGAGAAATCTCATGGTGTCATCTCACTGGGGTTTTAGAGGGAGAGGTTACCCAGATGCTTGCAATCTGC
    AGTGTTAAATCTTAAATCCCTGGAAACAGTTTCAACTTGTGTATATTTACTTTTATGTTTTAGTTTTTGG
    AATGCATGTCACAAATATTTATGAGAATTCTAATGGTGTCTCCCTTGTGGTGTATTTATTTTGGAAAATA
    TTTTAGGTGTCTGGCTGAGAAGGCAGGAAATGTTGCATTTTTGAAGGACTCCACTGTCTTGCAGAATACT
    GACGGTATGTGAAGATGTTTCGCTTCCTCCTTCAGGAGCAGTCTTTAGCACCCTTAACATATCCTCATTT
    TGGGGTTGGTCTTTGGAAAGATAGTGTAACTATTTCAGACTTTTGACTAAAAGAATTCTCAAATATTTTA
    AATCTATCTTCCTGCCCCCCCTCTTTCTCTTTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTTTCTCTCT
    CAAGGCAGAGTCTCATTTTATAACTCAACCTTGAACTTACTACATACCTCACATGGTCCTTGAGCTCATG
    GAAGTCTTCCTCTCTTGGCAAACTGAGTTTGGGAAATTACAGGCATGAACCACACCAATTGGTGAGCTCT
    CAAGTCTCTACTGCTGTAGGCAGCATAGAAACACCTGTAGTGTCACAGTGTTTTTGAAGCAATGCACACA
    CACAGGTACACACATACAGATATACACACATTCACATAGACATACACACTTACATGCATATATACACAAA
    CACACATATATGCATATATACATACACACATTCACACAGATGTACATACACATATACATAAGCATACATA
    CTACACACACTGAGCTGCTACACATGAGTATATACATGAATATGTATGTACATACATACATCCACATATA
    CATTCACACAGGCATACACACACACACACACACACACACACACACACACACATACACACCTGTCATAGTT
    GGTAGCAGGCACATGAATGAAGAAACTCACGGTCTGTCTGTACCAGATGAAACAAGAACAGACAGTAATA
    ATAACTGAACCCATTCAAATAAGATTTTATCTTGAAGATGGGAATAGAGGCATTTCTTCTTCTCATCTCC
    ATATTAAAACCTTGGTCTAGAAACTCCTTGAGAGTCATTGAGTAAGAGTCCACACGAACATGTAGGTTAT
    GAAGAGAATGTAGCAGCCAACATTAGAATAAGATCCCCCCCTCTTCTGGTCGCTAGATTGGGAAAACAAA
    ATAGGGTACCCAACTCTTGCCAGCTGTTTGCTACTGCTCAAGTTGCTCTTCCTTCCAGGTCTCCTCAGCC
    TCCATGCCTCTGCCTCTGAAGGCAACCTGCTGCTTTGTTCAGTAAACTCCAGTCCAGCCCCTTCTTCCTT
    TCCCTTCTGCTTGCCTGTTGTCTCTGTGTGTGTGTGTTTGTGTGTGTGTGCTTGTGTGTGTGTGTGTGTG
    TGTATGTGTGTGTGTTTGTGTGTGTGTGTATGTGTGTGTGTGTTTGTGTGTGTGAGGAGACCTCTGAGAC
    CGAAATGCTCAGTATTAAGTAGAGAAAACCCAGGAAGGGGATGAAGTGTTTCCCAAGGAAGGAGAGTCAA
    GCAGGAGCTTGGCTAATCAGCCTTTGTGTGCACATGGTGTGCCACATTTACACCCAGAACTCATGGCAGC
    CTGTGCGTGATACTCCCTGAGTATCATCAGACTGATGTCAAATTTAACTTTAAGGCTCTTAGGTACTTAC
    TACCTATGTGTATTGGAGTTAACTAGACCTATTGATAAGCCCATGGAGTTGCGCATTTTTTCCATGTTGT
    ACTGAAATGAGACGGAAAAGACTGTCTGTGAAAGTCTGAGCTTGGAAATGCTCTAGGATTGGAAAGTTTT
    CCCACACCACACGATATCTAACATGTGGGGGAATCCTGCACACTTGACCTCATCTGATGAGTCAGAGCCA
    GACTGTGGTCACTCTGGCAACACTAGGCAATATGACCTTCAGGTGACACCTGCAATTTGCATATGAAACA
    TAATGGAATATTTTGTTTATTTTTGCATTACCATGACCACAGTATTTCATTATGTACATACAAATGTTCA
    AAAACTTGAACAAATTTCAAAGCAAAAGTAGTTCTGGTTCCAAACGTTTTGAATAAAGGGAACTCAACCT
    GCTTTCTTTCTTTGGAAACTACTAGAAAATATATTGCATAAATGGAACTCTTTTGTAAAAGGAAGGGGCA
    GGAAGATACATCAGTGGGTAAAGTGCATGTTATGCAAGCATTAAGACTTGAGTTCGCACTTGGGAGGCAG
    AGACAGGCAAATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGCAGACAGAATGTCAT
    GGGCCTGCAGTCCCAGCTCTGAGGATGACAGGAATGGAGATAGGTGGATCCCCAGCCTTCTGGTCTAGCT
    GAATCAGTGAGCTCTGGGTTCTATGGAAAACTGTGTCTCAAAAAAAGAGCAAAGAAGCGATTGAGAAAGA
    TACCCATACGTAACTGCACAGGCAAGCATACCTGAACATATGTGTATGCACACATACAACACACACACAC
    ACACTTAAAAGAACATTTTTAAAAAGATTTACTTATTTACTCTGCATATAGTTTTCTGCTTGTAGGCCTG
    AAGAGGGCATCAGATCCCATTATAGATGGTTGTGAGCCACCATATGGTTGCTGGGAATTGAACTCAGGAC
    CTCTGGAAGAGCAGTCAGAGCTCTTAACCTCTGAGCTATCTCTTCAGCCCTTAAAAGAACATTTAACAGC
    TTGATTGGGATACAACTTGGTGATTTCAGAATCCACCCATTGTCTGTGTACAATCGAGTGGGCTCTAACA
    CACTCAGGATGGTGAAACCACCAATGCTACCATCCAACTCCACAACACTCATTTGAAAAACATGAAGCAC
    CCCATCTGTTGGCAGCCCACTGTCTTTGCTTTTTCAACATCTGCTTCCTGTTCCCATGGCTTTGTCTGTT
    CTCATAAATGCAACTATCATTTGTGGCCCTTTGTAGCTGGCTTCTTTATCATAGCAAAATTGTGAGAATC
    AGCCATGTTACCCCATGAATGAACTTCTTATTAAATAAGAATTTCCTTTATGACTAAGCTCGACATATTT
    GTCGCCTGAATAGCTTGTGAAGGATAAAGTTGCATTTTGTCTCCTGGACATCACCACAGCTTTCAAACTA
    GTCATACCTTGACCTAGCAGACTAGTTATGGCTAATGGTACCTGTCTTTTACCAGGGAAGAACACTGAAG
    AGTGGGCTAGGAACTTAAAGCTGAAGGACTTTGAGCTTTTGTGCCTTGATGACACCCGGAAACCTGTGAC
    TGAGGCTAAGAACTGCCACCTAGCCATAGCCCCAAACCATGCTGTAGTGTCTCGGACAGACAAGGTGGAA
    GTCCTTCAGCAGGTGCTGCTTGACCAACAGGTATGGACCAGCAGGGCTCCCAGCATGCTCTTCACTGCAC
    TGGCTCCTGCCGGGAGGGGGTTCATCCACTCAGACCTTTCTTTCTAGCCTTTCTGCTTGATCGATGGGGA
    TAGCGTTTGCTGCATGCTGTGAGTCTTTCTAGACTTCTGCCTGGATTCTCGGCTCCCAAACTTGAACTTT
    CTGACCTCTCCCTCCTGCTGCTTCCTCTAGTGACTCAGAGAGAGGGACGAACAGTGGCCAGGACAGAGGC
    TGTGAAGCAGTAAATCCAGAGTGTGTTCCATGTGCAGGAGACACGAGGTCTTTGTGCTCACAGATTAAAT
    ACTAGGAAAAGCAGGAATATTGAACATGTGGCGTTGTCTCTTCAAAAAAGAGATGTATGACCTATTTTTG
    CCAAGAAGCCTGGGAACAGGCGAGGTTAACAGCCTGGCGACTTCTACACTATCTGTATGGCCCAGCCATT
    CTGGATCCCAGACTATATGCCTGCCTCAGTCAGTCTATAACATTATCTTCTCTGAGCCTCTTATCCCTGA
    GCCTGGTTTTATAGAACCCATCTTTATGGAAGATGCCTTGTTCCTTACAAGAGTTATTCATGTAATCATG
    TCTTAAGCTTTGTTGTATACACAGGACAGTTTTTTCCCCCCTTAAATTGTACTGTGCTTAAATATGCCTG
    AAACAGACTACCCAGTGTCAGGGTTTTGAAGTTTGACCTAACACTGGCTACTGAGTTGTGTTGAACCAAA
    CTTCCTTCTTGCCTCAAACACAGTGCTGGCCCTGGTGTTTGTACGGAGCCCCGCGTCTGTGGATTTAGGG
    ATGACTGGGTTCTAGTGTTCCCTGTTCTAGTTTCTATTTTCCTATCACAGCATGTTGGCTCTGGGTACAC
    TTTTGAGGCCAACAGCCCTGAGTAAGGAGAAGGGACTGCAGCCGAGAGAGGCTCAATGCCCTGTGGTGGC
    CCAGTTCACAGAAACATGGGAATTTTTAAATTACCCGTTTGGACAGCTGGACACTACAATGTTCTAGATG
    ATCAGCTTTTGAGGGAAAATCTCAAAACATCTTCGTCTGAACTCTAATCACCAGCAGGAAGTGATAACAG
    ATTTAGTAAAAGGAAAGATATGGGAGGCAAGGTCAGAAGTGGTGTGAGGCTCCAGCCCTGGCTGAAGTGG
    GCATGCTGCCAGGGGGAGCACAGCACTGGGAGCACGGTGCTGGGAAGAGTCATCCCAGGCAGAGAAAAGA
    ACACTGCTGCTTTTTAGCTCTGCTGTTTACCCAAAATGGAGGAACCCTTATCATTCCCGCCCCCCACTCT
    CTGACTACTCACAGAACAGTCCACTCTCTTGACACAGGTTCAGTTTGGGAGAAATGGACAGAGGTGTCCA
    GGAGAGTTTTGCCTGTTCCAGTCTAAAACCAAAAACCTTCTGTTCAATGACAACACTGAGTGTCTGGCCA
    AGATCCCCGGCAAAACCACATCGGAGAAGTATCTGGGAAAGGAGTACGTCATAGCGACCGAGCGCCTGAA
    GCAGTGCTCCAGCTCCCGTGAGTGGGTTCTAGATATAATTGCCAGGGACCAACATGGGAGAAGCTCCGGG
    TTCGGAGGCCAAAATGCATGGAGAAGTGCAAGTTCTTAAAACCTTACAATGTGGAGCCAAAGAGATGGCT
    CAGGGGGTAAGAGACGGACTGCTCTTCCAGATGACACCGGTTCAGTCCAGAGCAACTGTCCATCTGGCAG
    CTCGCAGCTGTCTGTAACTCCAGTTCCAGGGAAGCTGACACTTCTTCTGGCCTCTGTAGGCACCAGGCCG
    GCATGTAGTCATCAGACATCCGTGCAGGCAAAGCGTTCACACACATGACACAATAAGTTCAAATGTAGGA
    AAGTATAGTGATAATAATTCCATTCTCGAAAACTGTTGGGAAGAGTGGCTGAAGAGTTAGTAGATTATTT
    GCCTCCCGTGCACAAAGCCCTGGGTCCAGCCTCTAGCATGGCATAAACTGTGTGGTGCACACCTGTAACC
    CCAGAACTCGAGAGGTAGAAACAAGACAACCAGATGTTCTAAGTCATCCTTGGCTACATATGGAGATCGA
    AACCAGCCTGGGGGACATAAAACCCTATCTCAAAAAAATTTCAAATACCTGTTATACATATATACATGCA
    TACACACACACACACATACACACACACATACACACACACACATATACACACACACATACACACACACATA
    TACACACACACATATACGCGCACACACACACATATACACACACATATATACACACACATATATACACACA
    CACACATACATACAGATTGCAATCCCCAAGCATCCCCACGCACCGATAGCCTGTGCACAGTAGCCCCTTG
    AATCCCTACATCTGGTGGTCCCTACTCTGAGAGGCAGAGGCATGGCAGATGTTGTGGTCCAGGAGTCGGA
    GCAGGCCCAGAATCTCCTTGTCAGTTGTTCTCAACTTAGGACAATTTTATCCCCAAAGCAGACATTTGGC
    CATGCCTAGAAACATCTTTGATGACCACAGTTTAGTAGGTGCTTCTGGTAGGAGGCAGTAGAGGTTGGGG
    TCATTAGCAAGCAATGCATAGGATACCACAGCCTACGAAAAAGGATTGTCAGGTTCCCTTTGCTGGTGGG
    CCTGAGGTGAGGAGCCACGATCTGTATTGAGCAACGTCTCAGGCTGTGGCAGGTGTGGTCCACCCTGGGT
    GACCCAGGGCTATTGATGAACCTGTGAACAGAAGGCACTGGTGCAGAGGCCATGTGCTCGAGGACAGAGC
    TGTCTGCCCATGTTCTCCCAGCTCATGGTGAAGTGGCTCACACAAGGCAGGTGCTCAGCCAGTATTTGCA
    GATGAAGGATAAGCAAACAGATCAGCCTTCATCGTGCACCATTCAGGAGCCAGAGGACCAGTGTCTTGTG
    AACTTGAGTGGTGTGAACTGATTCACTTAGAGGTGGAGTCACATGACCCTCAAGTCAGAGCAGGGAGTTG
    CTGTGGCCCAGGGCGCCACAGTTGCTAGAAGAGGTGGTAGAAAACATGTGGACGGACGAGGCAAGCCCTG
    ACTCACGATGGCAGGACAAGGGCTTGGACACTGTGTTTGAAACCTCTTTGATGTATAGATTTGGGGGGAA
    ATCTGTGTCTGAATGACCTGCTTTCTGTGTCCCACAGCACTCCTGGAAGCCTGCGCTTTTCTTACCCAGT
    GAAAACACTGAGCAAATAGCAGAACCTTCCCAGGAAGTCTCATCCCGGAGCCACGGTCCGGGGGCCTTCA
    GACCATCTGGTCTCCTCACTCCCTGCTGTCACTTTAGGTAGAAATAAAATGAAGTAGTGTTGAGTTTCTC
    GTCATATGAGGTGTCACTCATTGCTCCTCAAAGTTTATACTGGAATCCTGTGTCTGACCACTTGCCTGCA
    CACTTGGACTTGCTTTTGGAGATTCTCGGTCCAGCCCTTCCTTTATCCTGAAGTTTCAGGCATGGTGCCC
    ATTGACCTGGAGTCTGGGGACAACCACGTGATGTTTCTGTCCCCAGGGGATCTGGTTTCTTCACATAGAG
    ATCACAGTACATGTGCATCTGTGTGCCCCAGATCATGCGTGCGTATGGGTGACCATGTATGCCTCAACAT
    AAGTTTGCACATGACTGTGTGCACTCCTTGCACTGATGTTTGCATAGGACTGTGTACTTGCACTAATGCT
    TATGTGTGTGTGTGCACACACGTGTGAAAGCCAGAGGACAATCTGGAATGTCACCATCCACCTTTTTTAA
    AAATTAAATTTATTTATTTACATCC
  • Protein Sequence : Show Sequence
    >gi|31560677|ref|NP_032548.2| lactotransferrin precursor [Mus musculus]
    MRLLIPSLIFLEALGLCLAKATTVQWCAVSNSEEEKCLRWQNEMRKVGGPPLSCVKKSSTRQCIQAIVTN
    RADAMTLDGGTMFDAGKPPYKLRPVAAEVYGTKEQPRTHYYAVAVVKNSSNFHLNQLQGLRSCHTGIGRS
    AGWKIPIGTLRPYLNWNGPPASLEEAVSKFFSKSCVPGAQKDRFPNLCSSCAGTGANKCASSPEEPYSGY
    AGALRCLRDNAGDVAFTRGSTVFEELPNKAERDQYKLLCPDNTWKPVTEYKECHLAQVPSHAVVSRSTND
    KEEAIWELLRQSQEKFGKKQASGFQLFASPSGQKDLLFKESAIGFVRVPQKVDVGLYLTFSYTTSIQNLN
    KKQQDVIASKARVTWCAVGSEEKRKCDQWNRASRGRVTCISFPTTEDCIVAIMKGDADAMSLDGGYIYTA
    GKCGLVPVLAENQKSSKSNGLDCVNRPVEGYLAVAAVRREDAGFTWSSLRGKKSCHTAVDRTAGWNIPMG
    LLANQTRSCKFNEFFSQSCAPGADPKSNLCALCIGDEKGENKCAPNSKERYQGYTGALRCLAEKAGNVAF
    LKDSTVLQNTDGKNTEEWARNLKLKDFELLCLDDTRKPVTEAKNCHLAIAPNHAVVSRTDKVEVLQQVLL
    DQQVQFGRNGQRCPGEFCLFQSKTKNLLFNDNTECLAKIPGKTTSEKYLGKEYVIATERLKQCSSSPLLE
    ACAFLTQ
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
33. Nos2
  • Gene Name : Nos2
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 18126
  • NCBI Protein GI : 6754872
  • Locus Tag : RP23-341J22.2
  • Genbank Accession : AF427516
  • Protein Accession : NP_035057
  • Taxonomy ID : 10090
  • Gene Starting Position : 78920858
  • Gene Ending Position : 78960123
  • Gene Strand (Orientation) : +
  • Protein Name : nitric oxide synthase 2, inducible
  • Protein pI : 7.76
  • Protein Weight : 123528.57
  • Protein Length : 1144
  • Protein Note : Also known as iNOS; Nos-2; Nos2a; i-NOS; NOS-II
  • DNA Sequence : Show Sequence
    >gi|372099099:78920858-78960123 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
    TGGCCCCACGGGACACAGTGTCACTGGTTTGAAACTTCTCAGCCACCTTGGTGAAGGGACTGAGCTGTTA
    GAGACACTTCTGAGGCTCCTCACGCTTGGGTCTTGTTCACTCCACGGAGTAGCCTAGTCAACTGCAAGGT
    GAGTCTTGTGTTGGGGACAGCTGCTCTTAGGAGTGTCTTCCTGCTTGGGAAAGCCCAGAAAACCAGGCTT
    TCCCTTCTCCCCCCACCCCACCCCTCTTTTTGTGGCTGGACTGAAGCTGATATGGAAGGTGTAAGAAGCA
    CACCCACAGAAGGGTCTGCCAGTTGAACAGGGCGTGTGGTCAGATTTCTTCTCTGCAAATGTTTACTGTG
    GTGATTACACTACAAAGGATAAACTTTCTAGAAAGCTCCGAATAGGCTGGGGGGGGTGGTGTTAATACAT
    ATAGCTTACTTTACTCATCTTTATGAGATGTGCCCTCTGCCCCACCCAGATCTGATAAGATGGTTATTAT
    TAATCCCATTTTAGGAGTGAAGCAATGAGTTTCAGTTAACTAACTTGATTTAGATGAGTAACTAGAAAAA
    CTCAGGGTAGGGTCCAGTTCATCGGGACTCTGAGTTTCACTGTTCCCCTTAGGTGGGTTCTTGTACCCCC
    CCCCCCCGTATCTTCCAAGTATATCTTTCCTCAGGGAGGGCACTCGCCAAGGAGAAGCCACATACTCTCA
    GGGCAGTTCCACTTTCTATTTCTTGTCTTTTTCGTAAAGGATATAATCTCAACTCTGGAGAGTTTAATGC
    TCTTTTATACAAATCATTGACTTCTTCTCAAGTAACAAAGAAGAAGAAGGAACATGTGACCTAGTGTCCT
    TGGCTCAGGATGCGTGATGACTCCCAAATCAAAGGAAAGGTGGCCAGGACTTGAGTGCTAGCCCCAAGTG
    CAGGTCCAGAGGGAAGGGTACTAATGACAAGTGCTTGCCCCTCTCTCTGGTCAGCAGGTGGCAGGAAGGG
    TCAGGCAGATGCACACACTCCTGTATTAGTGACCCCAGCTCCTGCCCACCTTGCTGGAGGCCATGGAGTA
    ACTCCTGCCAAGAGAAACTGAATTGTCCTAAGGAAGCTTGTGGATGCTTGCTGTCCTTGGTAGGTGGCTA
    TGTGGCATCCCAAGTTATCCCAAGTTTGGTTTGTTATGTGTCTTTGTAGAACTGGAGTCTGTCCCACAAG
    ATTTCAACTCCAAATAAAACTGCCGAGTGCAAGCATGGAGAGAGAGCACTTCTGTGCTAGCCTTAGCTGG
    GCTCTGTCTCCATTCTCTGGGACACTAGCGCCCGCCCGTGAGAAGGCTCTGTTCTGTTAGGCTCTTCTTC
    AACCTCACCTCACAGCCTCCCTCCCCTTCCTCAGAGAACGGAGAACGTTGGATTTGGAGCAGAAGTGCAA
    AGTCTCAGACATGGCTTGCCCCTGGAAGTTTCTCTTCAAAGTCAAATCCTACCAAAGTGACCTGAAAGAG
    GAAAAGGACATTAACAACAACGTGAAGAAAACCCCTTGTGCTGTTCTCAGGTAAGCAACCTCTCTCCCAC
    TTCCAGCTTTCTCCTCAGGGCTTCCAGGCCCCTGCTGCCTCTCTCTGTTCTGGGCCACCTAAGAGAGTCT
    GGTCTCCAAGGCCCTGCTTGTAATACTCTACTGGAGTCTGATGCACCTTCCTTGGTGTGGACAAGGAAGG
    CCCAGGATCCCCATGCTGCTCCTGAGTGAGTCTGTACTCTCTGGAGGAAGAAGGAAGGGGGAAGGGGAAT
    ATGAGAGAACGGAGACTGGAGAGCCTAGTGGTCAGCTCAGTAGTGACGTCCTGATTGGCCAGGGCATGTG
    CCTCACCAGTTAGATATTTTGACTATTAGGCAACTGAAGGGTGTCAAGATGGCCCTGTACTATCCTCAGT
    GACTGTGTGACTTTGAGTAAGTGCACCTGTCTGAGTCTCAGGTCTGCAGTGTGATTTACCTGATAGTCAC
    AGAAGGGCTTGTCACCATTTAAGAGGCTTCTTTGTCACAGGACTACACCGTGCACACACCTACACTCTTT
    GAACGTAACCTTCCTGGGGCCCTTAGTTATGATCTCATTTTGCAAGTGAGGCTCTGGAGGGCAAAGTCTG
    GCTTACATGACTGCTAATGTCAGAGACGAAATCTGAGTTTGGCCTGAGGCATCTTAGAATTGAACTAGTC
    CTCCAACAATTTATCCGGATGGACTAGGAGAATTCTAGAGAATGCTAGGAAACTACAGCCATAGCCAGGG
    ACTTGAGGCTAACTTACGTAGAAAGATGATGAGCTGGCTTTCTTTGCAATGTGACTGCACAGAATCACGT
    CCAAGTCTCAGTCTGTACCTGGCTCAACATCTGCAGGACTACAGTGGACAGCGCCCCCTGGAGGCAGTAA
    GCAGGAAATCTCTAAATTTCCCTGTCCTGGTGTTGCCAGGTTTAGAAAGCAAGTTTAGGCAGAAGGGAGT
    AGCTTTGCCACTTCATAATCCTGTGGCATTTGTGACGTCCCTTAGCCTCCAAACCTCTGCCTCCTCACTT
    GCAGGAAAATAGTCATTATGTTAGGAGTAGAGGAGACAGATGGCTCAGGAGTGACAGGCAGAGTGAGTGG
    GAAGGGACTGGACCACAGCTGTCAGCAATCCTGGGGCTCAGGTGCCCTCTAGCACTTCAGTCTCAAAATA
    CCGTGACTTCATCCCGTTTTTTTCTCTTTACCCGGTCCCTACAACCCCTACCTTCTGTCGCCTCAGAATC
    CAGGTGCCTCCACACCTCTTTCCCTCCAACTTGACTTCATAGAAGTCATGCCCCTGCTCCAAAATCTTGG
    GTAGCTGCTGGTTACCTGTCATCACACTGAGACAAGAAGGAGGGCACATCCTCTGACAATCCAGAAGGGG
    ACTGGGTTGTCCTCCCACTTTAGGGAAGAGGAGACAAAAGTCCACCGTGCTTGAGTCATCTGAAGATGGC
    AGTGGTGAAGCAGGCATTCAAACTGGACAGTCTGGCTTATGGCCACCATCCTGGTAGCTACACTGTAGGT
    CTCCAATCGAGATGTATCCCAAGGACAAGGACACTTGAGGCTTTCTCACCTGGCACTATACTGCCAGTTT
    ACATGTCAACAGAGCCCTTCTGCTTCATCAGTGGATTGATGCAACCATTTATTAACTGCATATATGGCCA
    CACATATTTATAGTGTCCTTAACACTATGTCAAAAACCAGGCAGGAACTGCTTTTATCGTGTCTAAAGAG
    GTCAGTTTCTAGAAAGTGGAAGAGCTGAGATTTGAGCCTAACTCCTGTCTCTGAGGACTTAGTAAACTCT
    GGGCTGAGAAAGAGACAGATCCAGTCGCTACAGTCAGAGAGGAAACGGGTGGGGTGGGGGAGGGGGTTGC
    GCATGGAGAGCAACAGTTTCCTCCCTAGTTGGAGGCAGTCTTGGCAAGGCAAGATGTTAGAGAGGTTTAT
    GCGTCTCTGAGCCTCCTCCTAAGGGCACCGCCTGGCTGTGGGGTCAGGAGGGCCTAATGAGCTGCAGGTG
    GCTTTCTTAGAGGAGGGGGCCCAGCCGAGATGGGACTGCCCAGGAAAGGTGGCAAGAGGATGTTCCTGGA
    AGAGGAAGCCGCGTACAAAGGGGATTTTAGTCTGAAAGCAGTGGGAGCCCGTTATTTATTTTTATTTTAA
    GACATAATTTTCAAATCTATGCAGACGAAGAGCCTATTCCTGGTGGCTTTCAACTTTGTGTAAACAAGAG
    ATTCAGAAGTGTGCAGGGTGTGTCCTTGCATGTGTTTTAGAGACAAAGGATAAATGTGGGACCGTCTAGG
    TGACCCACAGGGAGTTTAGAGCAAAGGGTTTCAGGATGCTTAAAAGTACAAAACAAAACAAACAAAAACA
    CCCAAAACACTCCTCGCACTGTAATCGGGCTTGGGGATTCATTTGCTGAAATATTAGCCAGAAGGGTCCC
    TGAGGGCTGTAGCCCTATTTCACCTGCAACAGGGAGAAAGCGCAAAACATTTCCTGGGAGCGCTCTAGTG
    AAGCAAAGGTAAGAGATGACCCTAGGGCTGTTGTATGGCCTTGAGCAGGATCCTCATTTCTCTGGTCCCC
    CAGCTTCTGCCGCCATAAAGCAGGGGTAGTAAAGAATTGCCTTCTTAGTTGGATTTCGTTAGGGTGTGAG
    GACATAACCCGGTGCAGCGGGGAGTCTGGGTCGCGATCCTTACTGTCCCTTCGAGAGGAATACACAAACG
    AGGCACTCGCGCGCTCCTGCTTGTCCTGGGCCACTAAGAGCTGAGTCCTGCCCGCAGCAGGAGGGCGGGC
    GCTACGGGGGCGGGCGCTGAGGGGTTTCGCGGCGATAAAGGGACAGCGCCAAAGAGGAGCGCTGGCCCAG
    CTATGCGCGAGGGGCTGAGCAGCGCTTGCGACGCCGCCCGGCCGCGGCTCTGTCGCTTCCTGCGCAGGAT
    CTTCGGCCCGAAGGTCGCCAGTCGTGTGGGCCCAGCCAGGTACAGAGCGCGGGACCTGGGGAGGGGCCCG
    GGTGGCCCCGCTGGGACGCGAGAGAGGCGGGTCAGAGCGCCCACGCCGGCGGCCCTGTAAGGGTTTCTGC
    ACCAAGGAACTTTTGCGCTGCACTGCTTTGGACAGGTTCTAGCTATAACGATTACTTGGCGTCAAACCTG
    GAAATCTTCCTGCCTCGGCTTCTCAAATGCTGAGATTACAGGCGTGTGCCACCGCTTAAAGATGTTTTTT
    TGTTGGGGGGGGGGGGGGAAGCAGTACTGGGAACTGAACTGCATTTAAGCAAGTGCTCTTCCACTGAGCC
    ACTCTCCCCCTCCCGGCTTGCTGCTCTCCCTCCTTAATGCGCTCTGAACAAACAAGGTTAAGAAGCCAAA
    GATGATCTTTTACGATAAGACGTTCTCCCTTTCACAAGAGAGGTGGGCTGGCTGGTAGCAGTTCGATCCC
    AGGACTCAAAACCCTAAGTAAGCCCCTGCTGTGGGGTCTGCACTCACCCCTCAGTACCTAGTTCTGGGAC
    ACCGTGACAGCAGATGGTTCTATGGACCTGAGCAAGCCACCACAGTTCCCTTTGTCCGAAAGTCCATAAA
    GGTCGTTCTCCCTGCAATGGGCCAGTCAGGTCTCAGCAAGGCTGTGGGTTTAGAACTGCAGGAATTTGGA
    GCTAAGGGTCCCGAGCCTTAAAGTGTTCACCTGATATCTGGTACCTAGATTCCCTTTAACAGGTGGCAGA
    AGAAATCTGCTAGCAGGACATAATAGCTGGGGCAAAGCTTTATTTATCGTTGTGGGGTTTTCCCCTTAAT
    CTCTATTATAGCAGTACTTGAAAAATTATAAAAGTTCCAATTATAATAAAAACGTCAAAATACTTAAAAG
    TTAAAAAGAAAGAGATTTAAGTCACCCGTGGCTTATCACATCGTGCTAATCACTGTGAATCTTTGCTGGC
    ATTTCCCCCAGTTGCTTTCAGGCCTGCTGGAGCCTGCATTCATTCACACACAAGCACACATTGCATGCGT
    GCACAAAGACTGTCATCACAGCGTCACAGATATCCAGGCTGAGGCATTAGGCACAACCTCAGGCTGTGGA
    CCCTGAGGCCTGCCTAACTGTGGCTCATGTCAGCAGTGAGACTGCAGACAGCATTTAAAACATTTTGCCG
    TCTGGGGCAGGTTTACATCCTCACCAGAAGGAGGATCCCCAGAAAGCAAATCTTGGCTTCCGGATTTTTT
    GGGTTTTTTGTTTTTGTTTTTGTTTTTGTTTTTTTTCCTCCTTCTGGTGAGGTATACCAGTCCAGATCAG
    AGGAATATGTTAAAGAAAATATTATTCAGATGGGTGTGGTGGCACATGCCTGTAACCCCAACATTCAGAA
    GGTAACATCAAGAGTTCAAGGACATTGTCAGGCAAATGGTGAGTTCAAAGGCAGTGGAAGGTCATGAAAC
    ACTGTCACAAGCAAGAAGGAGAGAATTGTTCAATGAGTTTCTTGGTTTGCTTTGGTGGTGGCGGTGGGAA
    GTTATTGTTTTGCTTTGTTGTTTGAGATAGCCCCTCACCTGAGATAGCCCAGGCTAAGCAAGTCTCCTGC
    CTCACCTTCTGAATGCTGGGGTTTCAGACACCAGTCAGTGTATGTATCCCTCTTTAGTGATACCGGCTAA
    CATACCGCAAAGAGGACTTTCCTGAGGACTGTCCCTCAGGTGAGTCCAGGACCCACTGCAGTCCGTAGCA
    GAGGGAGAGATAGGGCTCCACCCAAACCCAGCAGGGGCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGG
    GCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGGGCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGGGC
    AAGGGCAAGTTACAGCCAAAGGTAAAGAAGAGCCTCACTGAACTGACCTAACAATCAAACCAGGGATCGA
    GTTATTACCAAAACACAAAGCAGGGCCATAGCTAAGCTGGAGTTTGGGGGGTTTGTTTTGTTTTGTTTTA
    GATTTATTTATGTGTATGGATGTTTTGTCTGACTGTATGGGTGTATAGCCATGGCTGCCCTGGAACTCTG
    TAGACCAGGCTAGCCTCGAACATAGAGCTCAGCCTGCCTCTGCCTCTTAAGTGCTGAGATTAAAGGGACC
    CACCACCATAGCTGGCTATATATATATATATATATATATACACACACACACACACATATATACATATATA
    TATATATATGCACACACACATATATATACACACACACACATATATATACACATATATATGTATATATGTG
    TGTGTTTCTTTTTATATTATATATATATAATTTTTATAAAATTAAATTTATTTTAATTTAATTTTTATCT
    TATTTTCAAAACAGGTAGCATATAGCCCAGGCTAGCTTTGAATTCCCTACTTAGCTGAGGATGGATGACC
    TTGAACTCCTGATCCCCCTGCCTCAACTTTCCAGGTGTTAGAATCATAAGTGTGTGCCTTCTGTACCTGC
    CTTTGTTCCTTTCACTCTTTACAAGCACGGGGCTTGAACCTGGAATCTCTGCACTCAAGAAGCCCTGGCA
    AGCGGATGGAGGCCAGTGCGGTGCACAGAGTAAACAAACGAAATCAAGGGTTTGGGGTGCTTTGTAGGTT
    CTCTTGGAGCCCAAACTGGCTTTGAAGTTTCTTTGTAGTTGAGGTTGGCCTCAAACTCCTGACCTTTCTG
    TCACAACCTGCCAGATGCTAGGATTCCAATTTGTAAAATGCCCAGCTTGAACTAAGAGATGAGCTGGGCC
    TGGTGATGCATGCTTTTAATCCCAGCACTCAGGAGCCACAGGCAGGTGGATCTCTGTGAGTTCAGGGCCA
    GTCTGGTCTACCTAGAAAGTCCCACATCTTAAACATACAATACTCACCAAAATGAAAACCGAAAATACAT
    AAGATAAAGTTTGCTAAGCCAAGTAGGTGGTGAGTGATGGACCCTGCTGGCCTGGGAGGGCTTTTGCACT
    TGGCCCTCTGAGGCAGGCATCTCCCCTGAACTTGTGTGTGTGCCTCTGTCTGAGTAGTTTTGGCTGGCTC
    CCCTCATATCATTTTGGCTTTGAGTGGTCAAAGCAAAGATGAAAAGGGAAGTGGGGACAAGATTCTCTAA
    GACTGGCTCCCTCATCCTTCCTGATTGGAAGCCGGGAACAGGTCATTCCCACCTCTAGCCCTTGGTACCC
    CGTCTGCCAGATCGGGACTCACAGAGGCCACTGGAGATCCCCTCCGTGGCCAGACTCCATCTCACAATGA
    CTGTTTCCTTTCGCAGCCCAACAATACAAGATGACCCTAAGAGTCACCAAAATGGCTCCCCGCAGCTCCT
    CACTGGGACAGCACAGGTCAGTGGTTTGGTCATACAGTATGGGAAGCCCTTCATTCTTTTCCTTGGTAGA
    TTCAGGCTAAACAGCCCTTTGGAAGCTGGGCAGGAAGGCACAGGAAGCCAAGGGCTGGGGAATAAGTGGA
    GCACCCCAGACTTAGGGTACAGAGGGAGCCAAGGAGGTTGCAGACAGTAGGACAGTCTTGATGCTCATCA
    ATAGAGGTCCCCAGGGTGCGCTGGTACCTAAGTAACTTGCCTGGTGGTTGACAGAATGGGTCGGGGTAGT
    GCCATTAGGGCTCTGTTTCTGTCAAGAGATAGCATGACCAAGGTAATTCTTTTAAAGGACTACATTTAAG
    TGGGGCTGGCTTACAGGTTCAGAGGTTCAGTCCATTATCATCAAGGTGGGAACATGGCAGCATCCAGGCA
    GGCATGGTGCAGGAAGAGCTGAGAGTTCTACATCTTGTTCTGAAAGCAAACTGGAGAAAACTGTCTCTTC
    CAGGCAGCTAGGAGAAGGGTCTCAAAGCCCACACCCAAAGTGACACACTTCCTCCAACAAGGCCACACCT
    ATAAGCAGTATCATTCCCTATGGGCCAAGCATATTCAAACCACCACAGGTAGTTTAGGAGCATTCTCTAA
    ATCAGGGAAGGACGGTTTTCCAGAATGATTTCAGATTCAGCTTCTGAGTCCCTAGTCTCAGCCCCTGGCT
    TCTTCCATCTTCCTGAAGCCCCTTACATCTTAAGGACTGTAGAGGGGTGGCAGAGAGATGGAGCCGGGAA
    AGGTGTCCCTGGGGAAGGGAGACAGTGGAGTATCTGTTGACTTCCCAGCTCTGCCCTTGGCAGTCCCAGC
    CACAGCTTCGGCCCTCCCATCAGACTTGAATTCCCCTTATGAGCCAGCACTGGCCAGGCCTTGGTAAGGA
    CAGAAGTCTGGCCTTGGTCCAGCCACCTGCAGTGCTCAGCTGGCACTAGCCCAACTTCTGGGAAGGGTGG
    AGGACCGAGGACTGGCCCCTTGCCTTGTGTGCTCACGCAGAGCTCCGTCCCTCATCCAAGCAGAGTACAG
    AGGCTCAGGGGACACTTGTATGGTTGTTGAGGTTATAGACATTTAGAAAACTCTTGATGTTTACCCATTT
    TAGAATGTTCCAGAATCCCTGGACAAGCTGCATGTGACATCGACCCGTCCACAGTATGTGAGGATCAAAA
    ACTGGGGCAGTGGAGAGATTTTGCATGACACTCTTCACCACAAGGCCACATCGGTAAGAAGTCTGGGGGC
    TGGGGAACACCACCTTTGTCCCAACTAGGCCATCATAAGGGCCCTTTGTGGGTCTATCACATATTTCCTG
    GTCTTATTAAACAGCCATCTTCTCAGACAAAAGATGTTGGGAAGTCTGTCTTTATAAATTATAGCAAGAA
    GAAGGAGAGGTTAACTACTGGGTTAGACAGCAGATAACCTAAGATGTCAGACAAAAAGCTACAGAAACCA
    GTAGAGTATCTGAGAATTGTCTATTAGAATTGCCCAAAGCTGGACTGAACTGGCAGAGGAAGCTTCCTGA
    CTCTTTAAGGACAGCAAGGTAAGCCAGGCAGTGGTGGCAAATGCCTTTAACCAGCACTTGGGAGGCAGAG
    GCAGGCAGATCTCTGAGTTCGAGGCCAGCCTGGTTTACAGAGTGAGTTCCAGGACAGCCAGGGCTATACA
    GAGAAACCCTGTCTCAGGAAAAAAAAAAAAAAAAAAGACAGTGAGGTAACAGTGGTTTAAGCAGCCAGTG
    AATGAAACTAATCACCCTTTCAGGTTCCTTTCTACCTTTAAGTACCCTACTCTGACTTTGGAATTCATGC
    TATGAGGCTGAGCAGTGCACCAGTGCCCACCTTTAATTCCTTTGATGGAGACAAGCAGATGTCCATGAAT
    CCTAGACCAGCCTGGGCTACATAGTGAGTTCAGGGACAAGCCAGAGCTACACTGCGAGACCTTGTCTCAG
    AACAACAATAATAACAACAAAACATAAAATAAGCCAGGTGTGGTATACACCTTTAATTTCAGGAGGCAGC
    AGAAGAGGCAAGTGGAACTCTGTGAGTTCGAGGCCAGTCTGGTCTACGAAGGAGTCCAGGACAGCCAGGG
    TTCTGAGAAACCTTGTCTCAAAACACCAAAACCAAAAAATAAAAAAATGAAAAGCCCATAAAACAAAACA
    AAGGGGGGAGAGAGAGAGGGAGGGAGGGAGGGAGAGAGAGAGAGAGAGAGGGAGAGAGAGAGAGAGAGAG
    AGAGAGAGAGAGAGAGAGAGAGAATAATTCATACTGTGGTTATAAAGTGGGAAAGAATCTTTGAAGGCAG
    GATGATTTGCTGAACCCCCAGAAACTGTGTCTATAATGCCAGCTTGGAAGATAGAGGCAGGAGGATTGGA
    AGCTCACAGTGGCCTGCTATGTAGTGAGTTTGAGACCAGCCTGAGTCACAGATGATCTTGTCTCAAAACC
    CAACAGCAAACCTCAGCAACAAAGCAAAACAGAGAGACGCCACAGTTGACATGTGGACATCCATCTTACT
    GGCATTTCTTTCCAAGCCTCCCCTGACTTTGGAATAACCATTCTCTCACTCAGCTTCTCTGGGCTGACAC
    TGGAGCCCTGTTCCCTGAGGAGCTAGCCGGGTCTCTTTAGAAAGGAAAGCAGACCCCCTAGGGAAAGATA
    TCCCCTAGGAGGAAGCAGTAGAGTTTTCTCACAGGCTGTCACTGTATCTTTAGCTTACTGGAGAACAGAG
    AGAAGCCAGCTCACACCCACCTAGTCTCAGGAAAGGAGAGGGGAGCTAGGCTTGGAGAAAGCTTGGTTGA
    CTCCACGGAGAAGGAGCCTGCATGGGGTCATTGTGAGCATACCGGCCAGAGAATGGCCAGGCTTATGACT
    GGTTTGGAGCATGGGGGGTCTCGGTGGGATGAAGTGGGGAATAGGGGAATAGGGGCCCTACTCAGCCCAT
    TTCCTTTTCCAGGATTTCACTTGCAAGTCCAAGTCTTGCTTGGGGTCCATCATGAACCCCAAGAGTTTGA
    CCAGAGGACCCAGAGACAAGCCTACCCCTCTGGAGGAGCTCCTGCCTCATGCCATTGAGTTCATCAACCA
    GTATTATGGCTCCTTTAAAGAGTAAGTCTGGCTTTCGTCAGGGTGACATCCCCTCTGCCCTACTCCCTGG
    GGCTGACCTGTTTCCTACTGTTCCACTCTGATCGGTGAACTCTGCAGTCTCCAGACTTGATGGGGCTAAA
    GTAGGCTCAGGCACTGCAAAAATGTCTGAAAGTTTGGAGGAGAGTGTAACCGGTTTTGGATTCCGGCGGG
    CCACCAGGTCACTGCTGAGTCCTGGAGAAATCCATTGGGTCTCAACCTCCTCATCTGTAAAGTGGGCATC
    TTATAGCATGACTCACTAGACAGCCATGAGATGTGTACAAATTTAGAACAGTGTTCTAGCCTAGGGACTA
    TAATACAGTGACTTGGAATGAATCCTTTTATCCTGAAATAGAAGGACATTTAGAGACTGTCTGGTCAGGC
    CTCTCCTCCTACAGATGTGAGCAAGATTCCCAGAGACAGCACTGGCATCAGCCTAGTCTAGCTAGAGTCC
    GGGAGACCTGACTTGCTGGGGTGCTACATCTCTGGAGTGCTCTCATTTTATGCCATAATGCTTCCTTGAG
    TCCAGTGTCCAGACCCCTTTATTCTTTGGGGTTTTGTTGTTGTTGTTTTGTTTTGTTTTGTTTTGTTTTT
    TGTTTTATTTATTTATTTGGTTGGTTGGTTGCAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGC
    TGGCCTCGAACTCAGAAATCCGCCTGCCTTTGTCTCCCAAGTGCTGGGATTAAAGGTGTGAGCCACCACT
    GCCCGGAGAGACCCTTTATTCTTAAGCCCACTAGTCAGTCACTCCAAGCACCAAGTATTGTCCAGCATGA
    AAGGCAATTGTTTTTCATTGCCAGTTGCCAATGTTGTTGTCAATGCAGTTGCAATCATGGCATGGCTGTG
    TGGCAACTCTTGGTAAGAGTTGGGATCCAAGGAGCCATCTTCTGAGGTTCAAGCCTTGGTTTTCTTGCCT
    CAAAATATCTATTATAAAATACCCACCTTGTAGGAGTTTATATGAAGATTAAATGAATTAGAGGCTTCAG
    AACAGTGCTGGCCTGTAATGAGTGCAACCGAGATCTTGGTTACCTCGTACGTACTCAAGCTTTTAAACAT
    CTGTTTACTTGGTGGAGAATGGGGTACCTGAGCCACAGCAAGTTTGTGAAGGTCAGACAAGCTGTGGGGG
    TTGGGCATCTCCATCCACAGTGCGGACTCCTGGGATTGACTGAGGTCATCAGACGTGGTATCAAATGTCT
    TTACCTGCTCAGCCCCTCATTGGCCCCTTTACCCTCTGGCCCCTCATTGTTTTGGAGACAGCATCTCATG
    AAATAATGAAGGCTGGCTTCTGCCCACAATTGTTCTTGAGAGTGCTGGGGTGACAGGTACAGGGGATGCT
    GTCTAATTATAGAACATTGTTGACTGACTGATTTACCTGTCCAGTTGTAAGAATGACTGTCAGAGACAGC
    GAATGTCTACTCTGGCAGCTGGGGACGGTGTGGCAGTGTCATTTTGTGGCACAGCCTCACAGAGCAGTTC
    CACCTGGGGCGTGAGACACAGAAGGGTATCTGGTACCCAGAGCAGAAGTTTCCATTCTTGGGGAATCCCT
    GTTCCACCTCTGTACAGCCCTGGAGTGCCAGAGCCGGGAGGGCGCTTGACAAGAGGTCTCTATCGTCTAG
    AGTAGTGCTTCCCAGCCTTACCTGGGAGCTGGCAGAACAGTGATGACTGGTCCCCCCTGAGCCAAGGTCA
    ACCATCTTGGGAAAGGCTGTCTGTAGATTTGTGAAGGGTGGAGGCCTGTGGGGGCACTGTCTTTCCTGTA
    AGCATGTATCCGGGGCTTTGTGGCATTGAACTTCAGTTTCCCAGATCTTCCCACTCTGTTGTTAACAAAT
    GGCTGTTTGGGGGGTCTGAGATTCTAGATAGGCTTCATCTGGCCATTTTTTTTAATTTGGCACCAAGCAC
    ATGAAAAACTCTGAGTTGCTGGGTTAACTCTGAGAGCCAGGTGTGGGCAACACAGCTCTCTATAGATAAA
    TAGGAAGATCTTAGGAGGACTCCAAGTTCAAGGATTGCGTGGGTGACAGGGTGAATACAAGCCAGCCTGA
    GCAACATAGAGGGAACATGTCTCAAAATAAACGGTTCAAAAAACAAGTGCTGGGGCCACAGCTGAGCATG
    TAGCTCAGTGGTAGGATAAGCCTGGTTTGGCGCCCTGCTTTTCTTTTGAGGCAGGTACTCACTATGTTGC
    CCAGGCTGGCCTGAGTTTACCTAATTTACACAAACTCATTTGGAAATTACAAGTGGGTGTGGAGAGACGA
    AGCCTGCCTATGGTTTGCTCCACGTGCCGAGTCCAGAAGGCCAGAGAAACAACTGTCTCCCTCCTGACAC
    TCCTGTGAAAGACAGAGGCTCCTGTTTCTTCTTGCTACCATAGTTACTGCGCTTTTCATTACAACAAGAG
    GATGGCTTTGCTCAGGACCTGTCCACTCCTTAGAATACAACGGGAGAGGCCAAAACTCTTCCAAAGAAAT
    CAGGCCCACTCCTGGGTAAATGACACCAAGTTTTCCTCTTTCTCCTTGAAGCGGTGTTGCCAGACCACAG
    ACACTGCTGTTTTCGTGTTTGGGTAGAGTGGGGTGTATTTCTAGAATCTTCCTCTGACGACTAAGGACAC
    TCACCTCACCCAGAAGTTCCCCCTCACAGGGATCACTAGAGACAAACATCGCTGAATGTTTTGTCTTCAA
    GTCTTTCTGTAGTCAAAAAGGAGTTGCCAAGACCCTCCGCCTGCCCCGAAGGCTGTTCATTCCAAGTCCT
    CCTGCTGACCTGAATCCCAAGTTCATTCTTTAAATAATTTTGTTTGTTGCCATTTCGAAAGCCTCTGGTG
    GTGCTGGTTGGAAATCTCAAGGTTGCTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
    TTCAAAGTCTTTGCGACCAGGCTCCTCTCCCTTGGAATTATTTTGAAGTATGCTTAAATCAGTGAGTGTA
    GCTCTGTTGGGTGGGCTCTAGGGTCCTTCAAGTTGAGTCCTGACCTCAGGTCTTGTCCGGGCCTGCCTTG
    AGTTTTGAGGAGCCCTCGAGGCTTGCTTCTCCCCTTGGGTGGAAGGTGGGAGGTCCTATCCACGACAGAC
    AGTTTGGAGCCTGGGGCTGGCAGTGAGACCACCTGGAACTATGTCCTCTCTGTTACTAGGCTTGTGTTCT
    TGAGCCTGGGACTGTATACTCTAGAGTCTTAAGACTGCTGAGCAAACACAGCAGTGCCTCCTAAGTGACT
    TGTGGTGGCATGTAGCTGCACTCTCAGGTATTTGAGAGGGGGAGGCCAGAGGGTTGGAGGTTCTAGGCTA
    GACAAGACCTTGTTATGACTATAATAATCAGCAGGGTCCAGCGTAGGAACATAAGTGTGGTTTTTCTCCC
    AACAGGACTCAAGATGGAGATGGCAGAGTATTAAAAATCGGCCCCTAGTAAGCGAAGAGTGGGTCACAAA
    GCCAGCCCTACCGAGGACAATGTCCCAAGGGGCACAATTTGCCCTTCTAGTGTCCAAAATCAGTTCTTGC
    TTTCTGGGGATTGAATTAATCTCCATGTAAGGTCTTGCTATTGTTGGTCTTCCTGACAATTTCCCGTCCC
    ACAGGGCAAAAATAGAGGAACATCTGGCCAGGCTGGAAGCTGTAACAAAGGAAATAGAAACAACAGGAAC
    CTACCAGCTCACTCTGGATGAGCTCATCTTTGCCACCAAGATGGCCTGGAGGAATGCCCCTCGCTGCATC
    GGCAGGATCCAGTGGTCCAACCTGCAGGTGGGTCTTACCGGCAGAGCAGAAGGAGGAGCAGGGGTGGGCA
    GGCCCTGAGACAGAGCAAGTGGCCAGGGCTCTGCAGACTACAGCTAAACCCTGAGGTGAGGCATCCCAGA
    CCCCCTTCTAGGAGCCTAGAATCTTATTTTAGCACCAGGGTCTCTAGATGACCCACCCTATATAAAATGT
    TGGGGACCACATGCCATCATGAGACAACCAGCAACCTTGGATGTCTTATTCCTGAGGCCTTGGTGCTACA
    AATGTTTGCCTCCAGTGTTTGCGATCCTGGCCCTAGAGAGCATTTTATTTATCGTGGCCTCCTAGGCCAT
    CGTCTCCTTGGAGGGTCATTTGGGCAGGGGTGGGGGGTTGGGTCCTATTGAGTGCACTTGGACTGAGAAA
    ATCCAGGAGTCTCAATGGTGGTGCACTCCCATGGCCAAGATCCAGGCTAGGGCTGTGGGGATCCTGCCTT
    TTGGTTCTTTTCTTCTTTTAAGATTTATTTATTTATTTTATGTATATGAGTACACCATTGCTCTCTTCAG
    ACACACCAGAAGAGGGCATCAGATCTCATTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGA
    ACTCAGGACCTCTGGAAGAGCAGTCAGTACTATTAACCGCTGAATCATCTCTCCAGCCCCTTTTGGTTCT
    TTAAAGCCAGACACTTTTGGTAATTTGGTTTTACTGGGGTCTTCTGAATGGGATTCTCTGATCCCTTGTC
    TCAAGAGTAGGGCAGCCTAGAGAAGGGCACAGGGGATGCAGAGAGGAGGGTGTGACCCTGTGAGAGAAAT
    GCCCTCTGTCCCCAAGGACTTTGAATCAGCCCCAAGCCTCTGTCCTCACAGTCTTCCATGGTGTGCCCTG
    TGCAGGTCTTTGACGCTCGGAACTGTAGCACAGCACAGGAAATGTTTCAGCACATCTGCAGACACATACT
    TTATGCCACCAACAATGGCAACATCAGGTGAGGCACCTTTCCCAGCTGTGCTGGGCCCAACTGACTACTC
    ACTCTCAGGAAGAAGATGCTGGAGAGCACAGGAGAGGGGCCTCTGGCTCCAGGAGAAACTAAGGAGAGAG
    AAAAGAATCCAAGACTTAGCTTTTCCAGGACATGAAATGGCAGGGCAGCCTCCTCCCCATTAGTCTCTGT
    TATAGCCCAGCTCTTCCCCGACCCCTTTCTAAGTTAGAACCTCAGGGCTGTTACATCTTTAGGAAATTAC
    TTTGAGCAGGCCATGGTGACACATGCCTGTTATCCAAGCACTTGGAAAGGTGGAAGCAGAAGGATCAAGG
    GTTCAAAGCCATCCTTGGCTACATAGCACGTTCAAGGCTAGCCTGGGCTCCATGTGTTCCTGCCTTAAAA
    ACAAAATAAAACCAAAACAAAGAAAACCATTTTCTTAGTTGGGCATGGTGGTACATAACAGCAATTTCAG
    CAGTGGAAGGCTGAGGCAGGAGGATTACCATGAGTTCCAGGCCAGTCTGGGTCACATTGACAGACCTTGT
    CTTGTTTTTAAAAAGAATCTACTCAGAAGGCAGAGGCGGGTGGATCTCTGTGAGTTCAAGGCTAGCCTAG
    TCTATATAGTGCTCAGTCTGTCATTCACTGTCATGCAAAAAACACGCACCTAATGGGGATTCAGCACAGG
    ACTAGGCCAGGGGCCAGGGAAAAGGAGGTGACAGGGTGGGATACAGAGGCGACTGAGACCCTAGAATGCA
    GCTCCATCCAGCTGCAAACCTATGGTGCACAGCCACCAAGGGTGAGAATAAAAGGATCCAGCAGCGAAAG
    CCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAGGGACATGAGGCTTCC
    CAGAGTGGTGGCCCCTTGTTGGTCTTGATAGTTAGGGAGAAGCTGGCAGGAGGAGGAAGAGGAGGGTGAT
    ATCAGGGTGATTGGGATGCAGGGAAGAGTGGAGGGAAGTTGGCTGGGAACCCCTGACCCACTCACCCCAA
    CCTGGGACAGTTGATAAACTACTCTGTCCCTGTTCTGGGGAAGGTCGGCCATCACTGTGTTCCCCCAGCG
    GAGTGACGGCAAACATGACTTCAGGCTCTGGAATTCACAGCTCATCCGGTACGCTGGCTACCAGATGCCC
    GATGGCACCATCAGAGGGGATGCTGCCACCTTGGAGTTCACCCAGGTACTGACTCAGCCTCTCCTAGATC
    CCTGTGGGCAGGGGACCCTGAGCAAATGTGGGGCCTGGCCCTGCAGACTTACTGGGCCCCTCTCTCCCTC
    AGTTGTGCATCGACCTAGGCTGGAAGCCCCGCTATGGCCGCTTTGATGTGCTGCCTCTGGTCTTGCAAGC
    TGATGGTCAAGATCCAGAGGTCTTTGAAATCCCTCCTGATCTTGTGTTGGAGGTGACCATGGAGCATCCC
    AAGTAAGTCACCCAGGGTACGGGTTATATGCATGCAAGCCCATCTCAGGCAGCACCCACAGGACAGATGC
    TGCAAGGCCTCTTTGCCCTCTGCAGCAGCTCTGGCCTTTCCTCTCCAGCCTCGCTTTATGTGGAACTGCT
    GATGGTTACCAAGGGAACAGCAGCCTAGCAAACAGGGTCCTGGGAGAGTGGGATGCAGTGACTTAAGGAA
    AGCCCCTGAGCTTCTGTCTGAGCAGAACCAGGCTTTTTTCTCACCTCCCAACAACTTCTTTAATTTTTTT
    CTTTTATTTATTTAGTGTGTGTATTTGAATGTGTGTGCTTGTGTGTGTACCTGTATGTGACTGTATGTGT
    GCATAGGTGTGCATGTTTGTGTGTGTGTGTATATGATATGTGAGGTATATGTGGTATGTTTATAATCATG
    TGTATGTTGTTTGTATGTATGGTGTGTGGTGTATGTTTATGTTTGTAAGTGTGGTGTGCTTGTATGTTTC
    TGTGTGTGATGTGTATGTGTGGTTATGTGTGTGTGTGTGTGTATGGTGTGTGTGTGTGTGCTCAGAGGTC
    ACTTGCAGGAGTCAGTTGATTTGCTCCTACCATATGGGTCCTAGGGACTGAACTCAGCTTATCTATCAGA
    CATGGTAGCTAGTGCCCTTACCTGCTGAGCCACCTCAATGGCCTCCTCCCAGCTTTCTTAGGAAAAGGAC
    AACCCTCCCAGACAGAGGAAGTGTAGGCTCACAAGGACTTTAAGAGTGCAGCTGGGCCAGGCAGTGGTGG
    CGCACGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCGAGGCCAGCCTGGTC
    TACAGAGCGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTTGGAAAACAAACAAACAAACA
    AACAAACAAACACGAGTGCAGCTGGTTGAACCTCTGCAAAATGCCTCCATATGTGGAGGTTTACCAGCTA
    GCAGGTACTGACAGCTCCTGTCCTTTGAACACACTGAACTCAGTCCTGCCTCAGGACCTCTGTCCTGCTT
    CCCTGCCTGCTATGCCCTTTCCTCGGAATTCTGCAAGGCTCCAGTTTTCCCATCAAAAGGTATTCACTAT
    GACATCACTGCACCTCGGAGAAGTCTCTCCTGATCTCCTATCTTCAACCCCACACCACACTTCACTTCGT
    TTCTCATTTTCTCACTGGTGTATTTATTTACCCCTCATAGTCTATAGTCAAACGGTCTATAGTCTGGATT
    CTGGGCTGCAACTTTGGGAATAGAATCTTACTTTTTATCTATAAAGTGCAGGGCACGTAGTAGGTGCTTA
    ATTATAGATATTTAGAATGTCTAACTATACAATGAATGATACTATGTTTGCAATAATCCCATGAGTTTGT
    TGTTATCGACTCTGTGTCTATGATGGAGATACTGAGGCTCCGAGGGGCTAAAAGGACCCTGGTCAGTGCT
    TGAACCCCAGCTGTCTGGGTGCCAGAGTACTTTGCTGCTACATTATGTTGGAGTCTCAGCATTTAGTCCG
    ATATACACCTGACTTATTAGCATGAATGAGTGAGTAAAGAGATGTCTTCTACAATAAATGACCCTAAGTG
    TTCCCTGTTATCTCTCGTGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGACT
    GAGATCATCTCTTCAAAAGGACTCTCTAGAGCTCGGGCTATGGCTCAGCTGAGCATGATTGCTTAGCATG
    CTTGAAGCCCTAGCTTTGCTCCCCAGCAGTGCATAAACTGGGTGTCGGTGATACATGCCTATAATTCAGG
    CCCTTGGGAGATAGAATCAGGAGGATCAAAAGTTCAAGGCCATCCTCAGGTACAGAAAATCAAGACCAGT
    CTTGGAAATGAGACCCTGTCTCAAAACAACAAAGAAAAAGAAAAGGAAGGATTTCTTTGGTTGATCCTTT
    TACTGAAGTTGATTTTTTTTGATTGGGGAGAGAGGGCAATAAAGATGGCCCTAAGTGCAGCTGGCTCTGC
    CTCCCTGCCAGGTACGAGTGGTTCCAGGAGCTCGGGTTGAAGTGGTATGCACTGCCTGCCGTGGCCAACA
    TGCTACTGGAGGTGGGTGGCCTCGAATTCCCAGCCTGCCCCTTCAATGGTTGGTACATGGGCACCGAGAT
    TGGAGTTCGAGACTTCTGTGACACACAGCGCTACAACATCCTGGAGGTGATGAGGGTTACCAAGCTGGGA
    CAGCTGGAGGATCAGGATGGGGGACCCACTCCACCCAAACTGCCTCACCCTCATCACTTGGCTTCTATAG
    GAAGTGGGCCGAAGGATGGGCCTGGAGACCCACACACTGGCCTCCCTCTGGAAAGACCGGGCTGTCACGG
    AGATCAATGTGGCTGTGCTCCATAGTTTCCAGGTATGCCAGGTATCTTGGGGGTTGGTAGAGGTTTTGGC
    AAGAAGGTCACTTGATCCAGGTACTTGACTGGCACTTGATGTCCCCTTTACAACATCATTCATTCATGTG
    TGCATTCATTCATGCTTGCTGGGTGCTGAGACCCAGCATTATTGCTGGTCTAGCTCTGGGCAACTCCTGT
    CCTCCCAAGGCTGCCTGCTCCCTGCTCCTGCACTTGAGCACTGTAGGCAGCTTGGCTTCCTTTGCTCCAG
    GACATTGCTTAGAGACCCCTCCTCCCGGCTGCATCTCCAGGGCCTTCCTCCCTGCCCCATCATTCCCACT
    GTGCACCAACAGTTCCTGCCAACCTCTGGCTCCTTCTTCTTCTCCTGCTCCTGTTACAGCCAATGAGACC
    CATCTCCTCACACAGCCTCTTCCTCCGCCACTTGCAGCCTCCTTTCTGACTCCCTGGGACATCTGAACTT
    CTTGCAGGGATCAAAGACTCAGGAAAAAAAGGGATCCTAACACAGGACCATAGCTCTCCTAAGCACATGC
    CATGGTGTGTGTAAAGGTCAGAGGACAGCTAACAGGACCCCGTTCTCTGCTTTCAGTATATGGGTTCTAG
    GGATCAAACCCAACTTGACTGGCTTGGCAGCAAGCACTTTTACTCCTGAGCCATCTCGCTGGCCCCCTTA
    CTTTTCCTCCCACCCCGTTTCTTCTTCTTTTAAATCACCCCACGACCAGGTCCTTCCCTCCTTGAAGGCT
    TCAGTCGTTCATGTCCTTAAAGAACCTCTCCTGGACCAGGCCACTTTGGGCAGTGCTCCCCCGTCCCCCA
    CACTGTCTTCTTTCCTTCCAACTCCCTTAACTTGACTTTGTTGAAGTGGTTCCCAGCACCAGGTCGTAGG
    TTGACGACCCCAATCTTTGTTGTTTCTTTCCTCCGCTGGAAATGTGAGCTGCGCAGTGCTCAGTGCTCCC
    TGTGGCCGGGATTGTGACTGAGTTCACTGCTACACCTCTAGTCTAGCTCATGATGGAGGATTGGGAATTA
    TGGAATGGATGGACAAGTGGAGGTGAGCCGCCCCCGCCCCCCCCACACACACACATGAGCAGGGGCCTTG
    TGTGCTAGGTGGACATGGTAGCTGGTTCCTGTGTGAACTGAGCCTTCTCTCCTCAGCTTTGCTTCTGTGG
    TTCTGTTTCCTGTGGAGCCTGGATATGTGAAGAGGTGGGGAGTCCAGTCAGGGTCATTCATTCTTTCAAC
    GGGGATTTAACAAATGTTTGCCCCATGTGTGCCATGCATTAGCTATGGGTTCATACAATGTCCTCCAGGA
    CTGGGACACTCTCTGGAAGATTAATTTTGTGCTCACTGGAGTAAAGGGTGGTGAGCTAACACAAAGAAGG
    AAGAGCCCCACCCCCAGCCCCTGGGGCAGGCCCAGGCAGGAAGGACCACCCCTGGTTCATACATCCTAAC
    CCCCAGGGACTCCCACCGCCAACCCTGACTCAAGCAGGATGCAGATGTGTCCCAGCAAATGCACAGAGAC
    ATAGTCACCCCCTGATGATGTCCCTCTACTGCCCAAGTCTCCAGGCCATTCTTGTCACCAACTTCTAGGA
    CACCCTTCAAAAAGAGATGGCCTTTTTCCCTTATAAAGTGAGGGCAGTAGACAGGTGTGATGGAGCACTA
    CTGTAAGTCTAGCATTCAGGAGGCGGATGCAAGAGGATGGTGAGTTCAAGGCCAGCCTGGGCAACTCCAT
    GAGTTATTTCTCAAAGAGGGAAACAAAACAAAAAATGGGAATAGCGCTTCCCTCGATGAGTCATTGCTAA
    GATTAACTGAACGTGAGACACCTTTGGAGCTCTCTTGTTCCCGAGGGGTGCTCAGCATACGTGGTTGGTC
    CATCTATGCTTGTCGGAGTTATCATCCTCTCCTGACATCCAAGGGCACTGTATATGTGCACAGATAGTAC
    CTGGCTTACGAAGGCTCCACTTGGGAATTTTTTACTTTACAAGGAAGGATATGAAGGCACCGTGAATCTA
    GAGCAACTCTACTTGGAGTTTTTAAAATTCTTTTTTTTTTTCATTTTATTTTATGTGTATGGATGTTTTG
    CCTGCACCACATGTGTGTAGTACCTGGCCAGAAGAGGGCATTATATCCTCTAGGACTGGAGTTAGAGGAT
    TGTGAGCTGCCATGTGGTTGCTGGGAATCAAACCAAGATCTTCTGGAAGAACAGTCAGTGCTCTTAACCA
    CTGAGCCATCCTTCCAGCCCCCTACTTGGCTTTGTTGTTGTTTGAGACCTTGACTTCTCTCTAAAACTCT
    TTTTTTTTTTGGTTTTTTATTTTGAGACAAGGTTACCCAGGCTGATCTTCAGTCACTCTGTTGTCTAGGC
    AGGCCTTAAACTCAAGGCAGCCTCCAGAGTCTCTGTGCTGATGGGCCTGTATGGTTAACCGCATAAGGAA
    TTTTAATTTCGATCTTTCCTGGGCTAACGATGATGGCCGGATGCTCTTTTGAAGCTGGACAGCAGCAGCA
    AGCTACAGCCTCAGCCAGCTATGGCAGCTACAAGGACTAGAAGCTGACACTATAGTGTGTTGTACAGTCA
    AGCTACGATGGTTGGGAGGCAAAGTGCTTTATCAAAAAGACTTGAGATGGAGTCTCATGTACCCTAGGCT
    GGCCTCACACCGGCTATGTAACCTCTGATCTTTTTGTCTACCTCCGTAGTGCTGGGATGACAGGTGTGAG
    CTACCACATCTGAGTCATTTGTTTGTCTTTTTTTTAATGTAGTACTGGGCATTGAACCTCCAGGTCTTGG
    TGCATGCTAGACCAGCAGGCTATGTTAGCTGAGCTACACCCCAGTCCCCATTCGATGTGTTCTTGACTGA
    GGATACTTTCATCTTGTGATGGGCCCATCAGCATGGAGCCCTATCATGAGTCAAGCAGCAGCTGTATATG
    AGTTTTAAAGTGTGTGGTTTGACTCATGAGCCTGACATCAGGTGGCAGCTGCCAACCTTGCCTCATCCTT
    CCCCAGGAATATCCCTTCCTTGGCCAACTTGAAACTATTTCTTAAGACTCTTTTCAGCTGTCCTGACTCC
    CACCTCTCCTCTAGACCTATCCCAGACACTAGGGTTTCCCCCTCTGTCCCCTCTCTGTCCCCTCTCTGTC
    CCTTCCAAGCAGCCTTTGCTTGCCTCTGTCACAAATCCCACTAGGCCTCATGAAAATAGCTGATTTCCTT
    CAGCCTTTCCAAGAGCCTTGCTGTTTGCTGGCAATACATGGAGACTGTCTCTCCTCTGCCCCATGGTCTT
    TCTCAGTACCTGACAGATGAACAGGAGCAGATAAGACCCTTTGAGAGCACAGGATGCTTCAGTGTTGGTT
    CTCTCCTTCCACTGTGTGAATTTCTGAGATGAAACTCAGGTTGGGCTTGGTGACCAACGCCCTTACCTAC
    TGAGCCCTCTCCCCAGTTGTAAAGAAATTTCTCATGGGTGAATGGATCTTCAGACTTTGATGGGGCCGCT
    ACTTTTACTGGATCATCCCAGGCTAGTTTAGGCCTCTAGTTTGTAAGATGTGAATGGCTTGTCTTCCCTA
    CACTCCGGTCCCCACCGTCCCCACAAACCACTCTGGTGGTTTTGTGTTTGCTTGTTTTGGAGACAGAATC
    TCTTGGAGCCCAGGCTGGCCTCAAACTCCCTACGTAGCTGAGGATGACCTAGAACTCCCAACCCTCATCC
    TGTACACCAGAGTGATGAGATTATAGTTGTCTAGTGCTTCTTGTATGCCAGGCAAGCACTCCACCAAGTG
    AGCCTCCTTTAAGTTTGTATTTTCTTTACCTGAAACAAGATGGTGCAGTGTGAGCTGCCCACACCTGAGG
    CTCTCTGTCTGGTGGCGATGCATGGAGACCTGTGCATCGAATTTCTCGATGCCGCTTTTAGGGTAGTGGT
    TCTCAACCTTCCTAATGCTGGGACCCTTTAATACACTTCCTCATGTTGTGGTGACCCCAACCATAGAGTT
    ATTTTCATTACTACTTTATAACTATAATTTTGCTACTGTTTGAATCATAATGTAAATATCTAAAATGCAT
    ATCTGATAAGTGACCCCCAAAGGAGGGGGAGGGAGAAGACACATAGGTTGAGAACGTGGGAACCTCTCAT
    GTGACCGCAGGCTGGCTGCCATATGTGTGACCGTGGTGATGGCCTAGGTCTCTAAGTCTGTGGACTTGGA
    CCTGTGTGTGTTTCTGCAGCAGTCAGTGAGAACATGAGGAGCAGGGGCAGAGTCATCGCGTAGATCACTG
    GCCCTCCAGTGCGGCACTTCCCGCTCTCCTCTGCTGCTGCATCTCAGTCCACTCCATCTTCTTCTCCTTC
    AGAAGCAGAATGTGACCATCATGGACCACCACACAGCCTCAGAGTCCTTCATGAAGCACATGCAGAATGA
    GTACCGGGCCCGTGGAGGCTGCCCGGCAGACTGGATTTGGCTGGTCCCTCCAGTGTCTGGGAGCATCACC
    CCTGTGTTCCACCAGGAGATGTTGAACTATGTCCTATCTCCATTCTACTACTACCAGGTCAGGGAGAAGC
    CTCCACCCACCTGCCCATCCCCGCCCCACCTCCCATCTTATAGCTCAGCTGTGGAGACTCTGTGGTGTGA
    GGACTCAGGGCTGTACATGCCAGCTGAGTGCCACCTTGTGCTCTCTAATCTAGTGTACCCAATAAAAAGA
    TACTAAAAGCAGGTGGGCCTCTACCTTCTGTGACCATGACAAAGGAAGCCAAGTGCCCACTGCTGTGTGG
    CTCATGTGGGGCCCAGGAAGCAGTACCCTGAGCTTTGGCTAACCCTTGGTGGAGTGGGCCTCTATGAGTG
    CAGTAGAGGGTCCTCTGCCTCTCCAGCATCCCCCGGCCCAAGCCCAGCCAGAACCCCACCCCCAACTCTC
    CTTCCTTTCTGCAGATCGAGCCCTGGAAGACCCACATCTGGCAGAATGAGAAGCTGAGGCCCAGGAGGAG
    AGAGATCCGATTTAGAGTCTTGGTGAAGTATGTGGAAGTGGGCATGTCCCTCTGTCTGTGCTGTGTCCCT
    TTTACTGAGGCATCCTTCCTGACTCCACCATGAAGGGCAGGGCATACGGAGAATGTCCCTTTTGGTGTTG
    GCTCCCAGAACTCTAGACACCAATTCTGAGACTGATTTGCTTCTGTAGTTCAGATTTGCCAAAGAGACTG
    AGTCAGGATGCACAGGGGCCACCAGTCAGAAAATGAGAAGTAGAGGTTCTGCCCCCCACCCCAGGGATAC
    CCCTGTTCCGTCTCTTCGGGACACTAACTCACCACTCAGCTGTCTGTGTATCTGTCTGTTCATCCCCACA
    GAGTGGTGTTCTTTGCTTCCATGCTAATGCGAAAGGTCATGGCTTCACGGGTCAGAGCCACAGTCCTCTT
    TGCTACTGAGACAGGGAAGTCTGAAGCACTAGCCAGGGACCTGGCCACCTTGTTCAGCTACGCCTTCAAC
    ACCAAGGTACCCACTTAGGTGGAAGGAGTCTGGAGTGAGTGTAAGCGGCCCCTTGGGAGCTGGGGGCTGA
    TGTTCAACGGTTGTCACACAGGTTGTCTGCATGGACCAGTATAAGGCAAGCACCTTGGAAGAGGAGCAAC
    TACTGCTGGTGGTGACAAGCACATTTGGGAATGGAGACTGTCCCAGCAATGGGCAGGTGGGTGGCCGGAG
    ACTCGGCAGAGAGGCTTGGGAGGACCAAGGAAAGGGTCCGGGGGACTCAGCATGACACTCTGGGTTCTTA
    TGCCACTTTCCCAATGGCACACAGTGTTTTCCTGTGAATTGACCAACTCTTACTTGTATGTGTTTAGATT
    ACTTGAGGGGATTCATTACATTTATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
    TGCAAGTCCCACAGCTTGCATGTAGAGGTTAGAGGACAACTTGAAGGAGACAATTCTTACCTTCTGTAAC
    ATGAGTCCCTGAGTTTTTCAGGCTTGGTGGCACTTTTACCTCCTGAGCCATCACTGCAGCCACAACCTAC
    TTAAAAAAATTTTTTTTCCAAAACTTCTTATTTAACTGGGCATTGTGATGTACACTCGCAATCCCAGCAC
    TAGGCAGTAGAGGCAGGAGGATCAGAAATTTGGGCGCAGCCTGGACTCCATAGGAAATCTTGACTCAAAT
    TCTCCCTCAACCAGGATTCCTCCTTAGGGGCTGAGAGATGGCTCAGCAGGTAAGATGGATTGTGGCACTT
    TCAAAGAACCACAGTCCAGTCCCAGCACCTATGTCAGGTGGCTCATAGTCTCCTATAACTTCAGCTCTAG
    GGACTCCAACATTGTCTTCTGGCCTTCCTAGGCACTCACACTCTGTACATAGACACAATCTCTGACACAA
    ATCTTTTTTGGGGGGGGGAGGGGTAAGGTTTGTTTGTTTGTTTATTTATTTAATGTAAGTGAGTACACTG
    TACAGATGGTTGTGAGCCTTCACGTGGTTGTTGGGAATTGAATTTTTAGGACCTATGCTCGCTCCAGTCA
    GCCCCCCTCACTCTGGCTCAAAGATTTATGTATTATTATATCTAAGTACACTGTAGCTGTCTTCAGACAC
    ACCAGAAGAGGGCGTCAGATCTCATTACGGGTGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTC
    AAGATCTGAGGAAGAACCCTTATTAAATTGCTCTTACCTTCTGAGCCACCTCACCAGCCCGACATAAATC
    CTTTAAAAAGAATTTTTTTTTAAATGTATTGTGGCTAGAGAGATGGCTTAGCTATTAAGAGCACTGACTG
    CTATTCCAGAGGTCCCGAGTTCAATTCCCAGCAACCACATGATGGCTCACAACTCACAATGTAATAAGAT
    CTGTTGCCCTCTCCTGGTGTGTCTGAAGACAGCTACAGTGTACTCACATACATAAAATAAATCTTAACAA
    ATGAGCAAAGACCGTATCATTCTCACAAAGGAAAAATGGGCCGAATATATGAATTTTGTCTAGCTACCCT
    CATGCAGGCCTGCACAGCCTTGTGTTTTGAGCAGAGACAAGTATGAGCAAGATGTTAAGGCTTGGTTAGG
    ACTAATTTGAGTTAGTCTCTCCTGTGTTGGAATGATGCAAAGGCCATCGAAAAGAAAGTGCATGAGAAGA
    GATTACAAAAGGCTTAGCTTCCCACAGTGTGATGAGTCCCACACTGCCTGAGACAGTTTCCTGGAAAATC
    AAAGTTATTTTAGAGAATCTGTTGTTTCTAGCAACTTCCAAGCAAGAAGCAAGGTTCGTCTTCTTTTTGA
    GGAACCTTGTGTTTTCCAAAGCTAATTGGGGAGGCTAGGGACTTCTGGGTCTTCCATTGAAGGGAAGGGC
    CCACGGGAGCTGGTTCAGCTGCAGTGAGGGGCAATCTGGACTTGTCCATTGGCTTTCCCTTAGTATCTGG
    ACTCTTTGCCTGCTCAGTGTGGCCAGTGTTTACACAGACCATCATAGTAAAGGAAAGCAGTGTTTTTGTA
    GCTCCTGGCCTGAGATCTCCGCTTCCTTGTCTTTTGTAGACTCTGAAGAAATCTCTGTTCATGCTTAGAG
    AACTCAACCACACCTTCAGGTGAGGCATCTCCCATATCCCTGACTCCTGGGCCCCAGTCTGGGATCAGAC
    CAAGGAACAGGGTGTACACAGGATGTCTTCTCAGACATGTCCAGGGGCAGACAGAGTTCCGGTCTGAGGT
    TTGTCTTCTGAGTGGGTTGAGGAGCAAGATGTGGAAACTGAGTCCCTCTTATAGATTTGCAGGCCTAGAG
    CCCACTGGTCACATGACCAAGGAAGGCCAGACTACCGTGTGACTCAGTGGGCATGGGTCTGTCCTTGCAA
    ACCATGGGCATTGATGCTGGGCATCCTGAGTCTCCATGTGTGTCCTTCTTGGCAGGTATGCTGTGTTTGG
    CCTTGGCTCCAGCATGTACCCTCAGTTCTGCGCCTTTGCTCATGACATCGACCAGAAGCTGTCCCACCTG
    GGAGCCTCTCAGCTTGCCCCAACAGGAGAAGGGGACGAACTCAGTGGGCAGGAGGATGCCTTCCGCAGCT
    GGGCTGTACAAACCTTCCGGGTGAGACAGGCTTTAATGAGTCTATGCCAAACACATCTGGGCCTGGTGTC
    CTGGGTGTCATGGCCTCTGCTAGGTGTAGGGCGGGGGTATGGGCCTGGCATCTCAGGTCAAAGCTGCTTC
    CCCAACCCTCACCCCACCAAGAAAAATGTCACTCTGGTGTTCTGCTTCAGGTGGGAGGTGATAGCTGTCC
    AGCTCCGGCCCTGACCTTATGTCTCCAGGGCTCTGAAAGCTTGGCCACCTCGTAGTAGTGAAGTCTTCCT
    TCAGTCTTCCAGCTCTGCAGAGGAAGGGACACAGGACACACTGCTGAGCTCTCGTGACCTGACTTTATCT
    TCTCAAATCTTTACTCTCTGGGTTCCTCCCTGCTGGTAAAGGAAGCTTAACACTGAAATGAATGGACAGC
    CATGGGAATCCCAGCAGACTTGATTCCAAGACTCAGGCCTCCTCTATGAGCTTTTCTATCAGGATAGGGA
    AACCCCAGACATCCCTTGCTATGTGGGAAGGGATAAATATAAGATGACAGCTTTACTATCCAGGAAAATA
    TCACTAACCACTGTGTGCCAAGCCTAGGATTCTCTGAGCGCCTCCAGCCCCTTTCAGATGGCACGTTGTG
    ATTCTGTGATGCCGCCATTCGGTATTTGACCCATGGTGAGGGGTGGTGACGGTGACGATGAATGCCATTC
    TGCCTAACTTTCCAGGCAGCCTGTGAGACCTTTGATGTCCGAAGCAAACATCACATTCAGATCCCGAAAC
    GCTTCACTTCCAATGCAACATGGGAGCCACAGCAATATAGGCTCATCCAGAGCCCGGAGCCTTTAGACCT
    CAACAGAGGTAAGAGCCCCTCTGCACCCTACTGGGCCTTCCCTGCTTGAGCTGAGGCTATCTGAGCCCTG
    AACCAGTAGTCAAAGAATCCACCATGCCCTTCTAATGAAGGCAGTCACCTGGAACTGCCAGGCAGCTGTC
    TCCCTGGCCACAGGGTCTGTTGGTACAGAGCTTCAGGCTCTGAGGGAGAGCGGGTCAGGAGCCCTGAGAG
    AGTGGTAGTGGGCACAGGGAGCTGGCATCAGCAGGTGGGTGGTGGCGATAGCTGTGCCAGGCACTGTGCC
    GGCCTCTTGGCCCACATGAACTGCCCACCAAGGGCTAAGGTAGCATGCAGGAATGCTCAGCTCAGCGTCT
    GGGGCTTTCCGCAGCACAGCACAGGAGATCTGTACCATTTCTGTCTGTCTTTCCAGCCCTCAGCAGCATC
    CATGCAAAGAACGTGTTTACCATGAGGCTGAAATCCCAGCAGAATCTGCAGAGTGAAAAGTCCAGGTAAG
    GATGGGCAGGGATGCTTAGCTGGTACCCAAGATTGCCCGCGAGATGCTTCTTGTGACAGAAGTGTGGCCA
    AGTACTCCATGCTCCCTGCACTGCTAGGGAAGGCTATAGCCAAACTCCTCTGGGAAACTCCAGAGTGCTG
    AGGGTCCCCAGCTGAGGGGATGGCTTGTCACAAGCTGGCTGGTGACCACACTTGGTGGTGGGAGAACACT
    TATCCTGGGGTAACTGGCAGTCACCCATGGCTCCCTTTTCTTCTGTAGCCGCACCACCCTCCTCGTTCAG
    CTCACCTTCGAGGGCAGCCGAGGGCCCAGCTACCTGCCTGGGGAACACCTGGGGATCTTCCCAGGCAACC
    AGACCGCCCTGGTGCAGGGAATCTTGGAGCGAGTTGTGGATTGTCCTACACCACACCAAACTGTGTGCCT
    GGAGGTTCTGGATGAGAGCGGTGAGCCCCCGGCCCTGGACAAATCTCTACTTCCCTCAGTCCCATCCCTG
    AGAACTGGGCCTGTGTCTGTCCTGATCTACTTCCATCCCAACTCATGGGAGAAGCTTTCTGGCCTTCACT
    GGTAGTGAGGCCTCCCCCTAGCCTTGGACGAGGCACTGGAGGAGCAAAACCTGGCAGTGTCCATCATCAT
    CCAGGAGAAAGGGAGTCAGCCTGAAACTTAGAGACAAGAGACTTGCATCCTTGGGGCTGTGAGAATACAG
    ATGTGGTCCAGATGAGATGCATTAATAATAAAGTCCTTGTCTCTAGAAGGAAGTGAGGGGAGTGGAGAGG
    AGTTAAAAGCAATGTGCTAACACGCACTAAACGTGCCCAGGGAACGGGCACTGTGCCAGCGATTTCCCAA
    ATGTAGTTTTTACTCATTGCCAGCACAGACTATGTCATCACATCTGTTTCTCAAGAAGCAGCTGGAGGTC
    ACAGGATTTGGACACTCGGCTGAGAAGTGGGAACCAGAGTTTGCGCCTGGGTCATGCCGTGACTCTCCGA
    ATGCTGCCTCTTGGGACTCCTGAAGCCCAGCCACCACAATTTACATTGATCTGTTAATATCTCAGGAGGT
    TGTAGCCATAAACCTCTCTCTAAAAAAGCAACCAGCCTCTGGGGCGTATATCAATCTCCAGTAGACTTAG
    TCACTAAGTTTGAGGTTCACCTGGGGATGTCAGAGACTCAAGTATGGGCCACAGAAGGATTGCTTAGTCT
    GTCAGGACAAAGCCAATATAGGTGCCCACGTGGGGGCAAATTTTCAGCCTACCCTGGCAGCCCTGTCTTG
    GCACCAGCTCCTGATCTTTGCTCTTTCTGGAGCAAAGATGTCAGAGTCTTCCCTCCCTTGCAGATGGAGA
    ATAGCCATCATCTGAGAGACCCAAGTAGGGCTTTCCCCACCCTGACACCTTTCTTTCTTCCATTCAGGCA
    GCTACTGGGTCAAAGACAAGAGGCTGCCCCCCTGCTCACTCAGCCAAGCCCTCACCTACTTCCTGGACAT
    TACGACCCCTCCCACCCAGCTGCAGCTCCACAAGCTGGCTCGCTTTGCCACGGACGAGACGGATAGGCAG
    AGATTGGAGGCCTTGTGTCAGGTGAGGCAGGGGCAATTCAGTGGAAGGAATAGAGCCCAGAGCAGAGTTC
    AACCTCTGAGACCATCCTCCTGAGTCCCTAGGTGTGCCTTGTCCGTGTCTGGTCGGGGCTAGGCGTCGTC
    TTCTGCCCCGTCTCTAGTACACAGCAGTATTGAGTGAATGAGTTTCTCTGGGAATCAGTATGCTGTAACC
    TGTATTCATCACATGTCCCATTTCTGTGACTAACTGCATGTCAGAAGGAACTTAAGGGAAGGAGGGTTCA
    TTTTGGTTCATGAGGGTCCAGTCCATCATGGTAGGGTGTCATGACAACAGGAGCATGAGTGACAGATCAC
    ATTGCTTCTGGAGTGAGAAAGCAGGAAAGGTGGACACCAGTGTCCTGCTCAAAGTCCCAGACCATAGGAA
    TTCGGGGTGGGTCTTCCCAGTCAAACCTCTAGAGAAACACCTTCACCAAGGTGTGTTTCCACAGTGATCT
    GAAATCCAGTCAGGTTCACTGTGAAGAGTAACAGCCAAAGTGCACTTCCTTAGAACCTGACTAAAGGGGG
    AAGCTCTATGGATGGCCTTTGTCTTCCCAAGTGTGGACCACGCTTGGTGGTGGGTAAATGGGGGTACTGG
    CGGGTGGGGAGCTGAGTTAATGAGTTAGTGGGGTGATGCATTAAGTCACTTGCGACTTTAGAGTCCATGT
    CTTTATAAGAGTCACTGGACAGCAAGCCATGGGACACATTGTCATTGTCTCAACAATCCTATATTAATGG
    CTGCATCCACCTAACTCCACAAGGATTAGGGTTAAGCTAAGGAGGGACATCCAGCCATGACTTGAACTCA
    GATCCAGTTCGTTCCAAGTTACAGTATAATAGAGAAATTAGCCCAGCTGGGTTGGGTGGTGCATTCTTGT
    GATCCCAGCACACAGGAGGCTGATGCAGGAGGATAGTGAGTTAGAGGCAAGTCTGGGTCAACAATGAGTT
    CAAGACCAACCTGGGCTATGTAGCAAGACCATGTCTCTACATCCTCCCCAAAGAAAGAGGACACTGAGCC
    CTGCCCATGAGTTGCTGATGTTGGGAGGCTTCTGTGTTTTGATTTTTGTCTTTGTTTTGTGGGTACTAGT
    GTTTTCCTTCAGACCTGCACACATGCCAGGCTCTACCACTGAGACAAACTCTCTCTTCTCTGACTTTTAA
    TTCTGAGGCAGGGTCCCACTAAGTTATCCAAGCTAGCTCTCAATTCGGTCTCTAACCCCTGCTGGTCTTA
    AACTTGGAATTCTCCTGCGTCAGCCTTCTAACTAGTTGGGGTTGCAATCCTGCCCCACTGTCATCTTAAT
    TCTATTCATAGTAGAGTTCTAGGCACAATTGAGAAATGCTACTAGCTGCCCCAGGTCCTTGCTTCCCGTC
    CACCGGAGTAGCCTCAGCACCCTGGATAGCGCCCGTCTTTTGCTGCGTTTGTTGAAGGAATGAATGGATA
    CTGGGTGGGGCAGCTCCTACACGCACGCAGCTCCTACACGCACGCGCGCACGCACGGCAGGTCCTCCGAT
    CCCTCACACTGCCCTTGCCTTTCAGCCCTCAGAGTACAATGACTGGAAGTTCAGCAACAACCCCACGTTC
    CTGGAGGTGCTTGAAGAGTTCCCTTCCTTGCATGTGCCCGCTGCCTTCCTGCTGTCGCAGCTCCCTATCT
    TGAAGCCCCGCTACTACTCCATCAGCTCCTCCCAGGACCACACCCCCTCGGAGGTTCACCTCACTGTGGC
    CGTGGTCACCTACCGCACCCGAGGTGAGACAGGCAGGCTCTGGTGTCATCTGCACCTGTCCTTCTAGGGA
    CAGCTGTCCTCGATTGCTTTCTGTTGCTGTGACCAACACTGACTGCCACCACTTTAGGGCAAGATCCAAT
    TTAGGGGTTTAACTTAAAGTTTACAACTGTCACCAAAGGAAGCCAAGGCAGGAACCACAGAGGAACGTTG
    CTTATTGGCTCCCTCTGGCTTGCTCAGCTGCCTTCCTTACCAGCTCAGCACCACCTGCCCAGGATCAGCA
    CTGCTCACAGTGGGCGATGCTCTTCTCCATCATCAATTAGCAGTTAAGAACGCACCCCACAGACATGCCG
    ATAGGCCTATCTGATGACAAGCCCTCAATTGAGGGTCCCTCTTCCCAGGTGTATCAAGTTGATGGCCGAC
    ACTGGTCACCACAACAGCTGTTCAATACCTTGGGGCTTTGTCAGCTACATGCTGCAGTGACAGCCACCTG
    CCAGAGTTGTCAGTGATATAAGATGCTGTGTAGGGCACAGTGTCACAAAGCAGGAGGGAAGCTCGCTCCT
    TTGTTTACCATTCAGACCTGTAGGAGATGGCTTCCATGTGTAATTTTACGAGGAAGGAAAAGAGTCATGG
    AGAGGTTAACCCACCTGTTCAGAGACACCTGTCATTAACTAGAGACACAAATTCAGGGAGTTTACCTGCT
    GAAGCTAAGAACTGGACCATTGTTACAGTCTCTGGCTGTTGAATAGGCTCAGACGTGAAAAGCCTTGAAT
    GCGTAGGGAACTCAGTTACTTTTGGTCATCCTAATGATGCAGGTGGATGCAGGGAACATTTTCTCAATTT
    TTTTCATTCCCTCAGTGTTCCCATCCTGTGATGGCCGTGTCTCACCTCCATGCCAGGGGCCACACTGGGT
    GTGGAAACTGCAGACAGGCCCTGCCCTCACAGAGCAGCCAGAAAGTCAAAAAAGAGTTCAGGCATGGACA
    TACTTGGACATATGTTTGTGTGGGTGCTCAGAGGCCCCCGGTATCACGAGGGAGGGAGTGGGGTGTGTGT
    GAAGCTGCCCTAGACTAGGAGGTCAAGTGTTCTAGGCTTGGTTCCTCCACTGGATGGACCTCCCTGTTCT
    AACCACTGTAACTGAGCTCTGTTTGAGCTGGAAGGGCTGGAAAGCGTCCACATCTCCGAGATCTCTCTCC
    TTGGCCAAGAGCGCAGTTTGTAAATACTGCACAGAGCCTGTGTCTGTCCATCCTCACTGACCATACCACA
    CCATTGAATGAGAGAGGAGATGCGGGTGAGCCCTGACAAGGCTGGTTCATGCAGACCAGAACCAAACAAC
    TATTCCAGTTGCTAAAGGCCTCCCTTATGTGTCAGGCCATGGGGAAGTGAATGTAACCCAGAGGACACTG
    GGGTACCCACAAGCCCAGGAAAAGGCTTCTGGGGAAGATGGAGATGCCAGAATAGACAGGGGGCGTGGAA
    GACCACCCCTCACCCAGAGTTCCTATTGGCAAAGGCCTCCTTCATGAGGACACTCAGCAATGGCCAGCCC
    TGGGGATCATTTCACACTGCGTGGGCAGAGCCTTAGTGGGGAAAGGATGTTGGGAGGGTCCCTAGACAAG
    CACTTGGGAGATGCTCTAGCGTAAGACTGAGGAACCTGAGCAGGAAGTTGAGTCCACCATGACTTTAGTC
    CCTTGCTCCGTTGCCTTCTGTGTGACTCAGTTTCTACAATGGCAAAGGCGCAGTTGCTCACCAGGCCCCT
    GACTGCCTTCCCTGATCTGTATAGACAGCTGGGAGAAGAGGTAACTCCTCATTCAGGAAGCAGCCACCCA
    ACGGGGCTTGACACTGCAGGAAGAACACCCAGCCGGGTTGCACGAAGCCCACGGGGCTTGTTAGTCAGGC
    CTGTGTTTTCCTTGCAGATGGTCAGGGTCCCCTGCACCATGGTGTCTGCAGCACTTGGATCAGGAACCTG
    AAGCCCCAGGACCCAGTGCCCTGCTTTGTGCGAAGGTAAGCACCCACTCGGGGCCCTTTGGAGCCCCTAG
    CCCCTGGGGATTCTGCAATGCAAGGGAAGGGTCTAAGACTGAGTCTCCTCTCGGCCCATAAAGGTTTAGG
    GGGTAATTCTTTGTCTCCTCGTTTTGCCATCTGGTTACAAGGTGTCGTGGAGTCCATGACACAGATACAG
    TGATGCCTGTGGTGGGATCCAATGAGACTGGCCCAGTGTGGAGAGAGGCCGTTGCCTGGGCTGGGAAGAT
    GAAGATGTGCACAGGTCCCCGCCAGGCGCTTAGGTCCCCAGCCCTATCCTGCATGTGCTCCTAGCCTGGG
    ATATCTGCAGACCAGGGTCTTTCTCCTTCTGTCTTCCTGCAGTGTCAGTGGCTTCCAGCTCCCTGAGGAC
    CCCTCCCAGCCTTGCATCCTCATTGGGCCTGGTACGGGCATTGCTCCCTTCCGAAGTTTCTGGCAGCAGC
    GGCTCCATGACTCCCAGCACAAAGGTACAGCTCTTACTCAATAGGGAGTGTGCCTGTCACTGTGACCGAC
    CTGATCATTACACAGGCACATCAAATTACTACACCATATCCCATAATCAGGTACACAGATGTGGAGTGTT
    TGTGCTCTGCCCACTCCACGTCTCCATGTTTCCTTGTTAATTTTTAAGGTATCTAGAGGAGTGTGTATGT
    CCTGGTACGCATGTGGAGGTCAGAGGTCAACTTGTTTTACTTCTTCCAGGAATCAGATTCAGCTTGTCAG
    TCACGACGGCAAGCACCTCTCCCTGCTGAGCCATCTCATCAGCCCATTTTGCTGGGTTTTAAATGCTTGC
    CTCCTTGCTGGACCATCCATTAATAACACAGAGATTAATAAGCCTGTGTAGGGGTCCCCAGAGTGAAGGG
    GGAATGAGAGTAACAGACTTTAGATGAAGACATTTTCTTTTCATTGGAGAAATAGCTGGGAAGGATGGAC
    AGCTCTGTCTGGAATCCTCCAGATGAGGCTTTTCTGGAAGAAACCCTTAGCAATGTTTGACCTTCAAGAT
    GCCTGAGGCCTCTGCACCTATATTTGATGTAGCATCTGGGTCAGGGGAGATGATTTTCACAAAAGAAGGC
    CACGAGTTTCTGATATTCCACTAAAAGAATGTCCTTAGCATGTTACACACACACACACCTATACATATTT
    GAGACAAAGTCTTGCTATTGTAGCCCTGGATGAACTGAAAATTCCCTATACAGAGGAGGCTGTCCTCAAA
    TTTGCAGGAAATCAATCCTCCTGCTTCTAATTCCCAAGAACTGGCTATAATAATATTTTAAAAGAAGAAG
    TAAGATTAAAATAGAATATGTCAAGTAAAAGAAATAGCAATGTGTAGACATAATCAGAACTGATACTTTT
    TTTTCCTGAACCATGTTTTTTAAGAGTGCCACCATTAGCTATAAAATGGGAATAATGCCAGATCAGAAAC
    CTACCCATTGGGTACCTGGTGCTCACAGGTTGCCCTCTGGTGGCCGTTGTGGTAACTGCAACCTTCTGGA
    TCTTCAAGGGCTCAAAGGAGGCCGCATGAGCTTGGTGTTTGGGTGCCGGCACCCGGAGGAGGACCACCTC
    TATCAGGAAGAAATGCAGGAGATGGTCCGCAAGAGAGTGCTGTTCCAGGTGCACACAGGCTACTCCCGGC
    TGCCCGGCAAACCCAAGGTAAGTGCAGCCACCCAGCAAGCTGGCAGAGCACGCATGATGGCCCCTGCCCA
    GGCTATACAGCCCTTCCACTCCAAGCCTGCAGTGAAGACAGTTGCACAGTGGCAGGGAGAGATGAAGAGC
    ACGATGACAACATCAAGCTCACTGTAGTTTAATCTCCACAGTGCAAACACCTGCAGGTGCCCAGCATCTC
    TGGGACGCAGGGCTGGGCCGGGCTGGCTTTACACTTAGTTCAGTAAGACTGGAAGGTGGAGCTGATTCCC
    CCCAGGGCCCTCAGGTTGGCACTCTGCTCCAGGGGCATGAACAGTGCCTACACACTTTCCATTCAGGACC
    ATGTGAACATTCCCTGAAGACCCCATTTGCATCCTAAAGCTGGACCACTGGTCATCTTAGATGAAAAACA
    AAAACAAACAGACTCCTGACTGACTGCAGGACCTTCTTTATAAATGGAAAGCCTAGGAGCCTATACCTGA
    GCTACAAATCAGAGCTGGGGCTAATACATACTGGGAAGCCCCCTCTTTCCGGGGTGTAGACAGGCAGATC
    TCACAGCCCTGGGCAGAGCTATAAACTTGATGTCTCTAAAGTATGGTGACCTGAGGGAAGCTTTACGGGT
    TCCATTGGCCAGGAGGACAGGATACTTGTCTATCCTGAGCTGAGGTCCTGACTGAAGCCCTCAATGGCCC
    TCTTCCCCTCCCCATGGCCAGTGTGCCTGACCACAGTGACACCAGCATCCAGGGCTGGTCCCACCGTGTC
    CTCTGCTTTTAGGTCTACGTTCAGGACATCCTGCAAAAGCAGCTGGCCAATGAGGTACTCAGCGTGCTCC
    ACGGGGAGCAGGGCCACCTCTACATTTGCGGAGATGTGCGCATGGCTCGGGATGTGGCTACCACATTGAA
    GAAGCTGGTGGCCACCAAGCTGAACTTGAGCGAGGAGCAGGTGGAAGACTATTTCTTCCAGCTCAAGGTA
    TGGGAGCTGGGGTGGGACCCTCCAGAGGGGAGTAGACCAAGGTCACAAGCCTTTGGAACTAGGACCAGGG
    ATGCAGAACTGGGGTGGGGGAGGTGTGTGCCCTGAGATCCTCGGCACCACTGCAGAAACCTGGGGCCGGC
    TGGGCTCCTCAAGCCTGGTCTTTCCATGCCATAGTGGTTGCCAGCTAGCTGAGAAATCAGGCCAGTGTGT
    TCCATTTCTCTATATCTGGGATGAAAGCAGCAGTCTCTGCTCCCCACACTCCTGCCTAAATGCCCATTGA
    GCATAACAGGGCTGTGGACTGTCCCCTACTGCCTATGGGGGACAGACACTTATTTACAATAGGTTGCTGG
    CTAGGTGCCCTCATTTCTCACAGGAAGGACTCTTGGTAGCAGGGTCTGCCTTGTGCCAGGTGCTCACCAA
    TGATTGCCCCGTGGTGACCTTTCCTTCTGCCCTTCCAAGGGCTTTATGACGGAAGAATGACTGTTCCATC
    ATTTTGCAGATGGAAGGGGCCCTGGTGCCCTAACAGCGAATGTGAGAGCCCAGACACATGGCCCCTCTCT
    CCTTTTCCCTCTCTCTGTCTCTCTGGTTCCTAAACCTGCCTTTAAGGCTCGAAGTTATGCTCCCCATTTA
    TATTAAACATCTACTATGTGTTAGAGGTAAGAAGCAGTCAACAAAACAGGTGAAAATGTTGTCAATATCA
    CGATAGATCACAGAGCACACCTGTATCGCCAGCACTGGGGAGGTGGAGGCCCAGGGGTCAAGAGTTCAGA
    TTGTCCCTGGGTTTCATAGTCAGTCCAAGGCTGGACTGGGCTATATGAGATCCTATTTCGAAAGTACAAA
    AGCAAAAGACCTACCTAAACGTAGCTTACATTCTGGCAGAGTTGATCAAATCAAGCACATAGGGTGCGCA
    CTGCTTTGGGTAAGACGCAGAACAGCAGCTCAGAGCTGGGGTGTTTGTGGTTTAATGAGGTTTGGGGAAG
    GGGTCCCCGGGAGGTGACATTTGAGAAAAATACCTGAAGAAGGAAGAAGCTTTGTGCACACTGGAGACAG
    AGGTCCAGACTATTCTGTGGGGCAGCAACATGTTCAAAGGTCCTGGGGTGTGGACTATTCTGAAACCTGT
    GATGACCAGCAAGGGTATCATGGGTACTTCCCAAAATGTGTTCAGAGCTTGCCAGATGTGTTCATGGATG
    TTAGACATCATTAACAATGGGCTGTAAGGGCAGAGGCAGTGCACACCTGTTAGTCCTAAGGCTTTGGGGA
    AGCTGAGGCAGGAGAGTTGTAAGTTCAAGTCAGTATGGGCTACCTTAGGCTGCAAGAATGCAGAGGACAT
    ACCTGTAACATCAGCTCCAGGGGATCTGATCCCTCATATACAGGTACATGTATACACACACACACACACA
    CACACACACTTGAAAATAAAACTGTAGCCAGACATGGAGGCATGTGCACACCTTTAACTCTAGCACTCAG
    AGGCAGGAAATAAAATCCTTTCTTTTTTATTTATTTTAATTTAAAAAGAGAAAATTAGTTCCAGGGGTAT
    AGCTTAGGCCTTGCCTAGCCTGGTATATAAAAGGCCCCAGGTTTGATTTCTAGTACCAAGGGAAAAAAAG
    ATTCCAAGGCCAGACATTTGGTACACACGTGCGCACCCACAAAGAGCAACAGGTGTCTGCTGTAAGATTT
    CTCTCGGAGCCCTCAGCCTGCTGCTGATTTCAAGGCCTGAAGAATGGGAGGCACACACTGCCTGCCCTGC
    ACATCCTGATCTCAAAGCTCACATCCGATGTGGGATGAGCACTAGACAGGTAGTGGGACCGACATCCATC
    CATGAAGTCTGGGAAGAGGCTGAGGTGGGCCACACATGGGCAGAGGAGAAACCAAGCATGGGTGTAATGG
    CAGCAATCCAGACAAGGGGGTAGACACAGGGCTAGCGAGCGCCTGTGTCACCAGGAAGCTGTGGCAGCCC
    TAGGGTTTTGTCCAAGCCTAAACATGATAACTGAGAGGTGGCTGGGCCAGCAGGGTGACACTGTCACCTT
    TGTTTCCCCCTCACAGAGCCAGAAACGTTATCATGAAGATATCTTCGGTGCAGTCTTTTCCTATGGGGCA
    AAAAAGGGCAGCGCCTTGGAGGAGCCCAAAGCCACGAGGCTCTGACAGCCCAGAGTTCCAGCTTCTGGCA
    CTGAGTAAAGATAATGGTGAGGGGCTTGGGGAGACAGCGAAATGCAATCCCCCCCAAGCCCCTCATGTCA
    TTCCCCCCTCCTCCACCCTACCAAGTAGTATTGTACTATTGTGGACTACTAAATCTCTCTCCTCTCCTCC
    CTCCCCTCTCTCCCTTTCCTCCCTTCTTCTCCACTCCCCAGCTCCCTCCTTCTCCTTCTCCTCCTTTGCC
    TCTCACTCTTCCTTGGAGCTGAGAGCAGAGAAAAACTCAACCTCCTGACTGAAGCACTTTGGGTGACCAC
    CAGGAGGCACCATGCCGCCGCTCTAATACTTAGCTGCACTATGTACAGATATTTATACTTCATATT
  • Protein Sequence : Show Sequence
    >gi|6754872|ref|NP_035057.1| nitric oxide synthase, inducible [Mus musculus]
    MACPWKFLFKVKSYQSDLKEEKDINNNVKKTPCAVLSPTIQDDPKSHQNGSPQLLTGTAQNVPESLDKLH
    VTSTRPQYVRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIEF
    INQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDARN
    CSTAQEMFQHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLEF
    TQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANML
    LEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHSF
    QKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEPW
    KTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAFN
    TKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFCA
    FAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWEP
    QQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIF
    PGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKL
    ARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDH
    TPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTGI
    APFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVYV
    QDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHED
    IFGAVFSYGAKKGSALEEPKATRL
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis fadD26 mutant vaccine , Mycobacterium tuberculosis sigE mutant vaccine
34. PCDHB1
  • Gene Name : PCDHB1
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 29930
  • NCBI Protein GI : 14195607
  • Genbank Accession : AC010223
  • Protein Accession : NP_037472
  • Taxonomy ID : 9606
  • Chromosome No : 5
  • Gene Starting Position : 19530
  • Gene Ending Position : 22063
  • Gene Strand (Orientation) : +
  • Protein Name : protocadherin beta 1
  • Protein pI : 5.03
  • Protein Weight : 82950.69
  • Protein Length : 818
  • Protein Note : Also known as PCDH-BETA1; MGC138301; MGC138303
  • DNA Sequence : Show Sequence
    >gi|28558729:19530-22063 Homo sapiens protocadherin beta cluster (PCDHB@) on chromosome 5
    CAGTAACCTGTTGCAGAAAAGTGAAAGTATATCCGCAACAGTTGGCTCTGATTGCAGAGAGCGCGCTTGT
    GAGAACTGATGGCGGGTACGCGCAGAAAATCTTTGCAAAACAGGCAAGTGGGATCTCTTCTCATTTTTCT
    GTGCATATCTGTGGGGGATGCGACAACTATCCGCTATTCAGTGGCAGAGGAAATGGAGAGCGGCTCGTTT
    GTGGCCAACGTAGCTAAGGACCTAGGACTGGAGGTAGGGAAGCTGGCTGCGCGCGGGGCGCGGCTGGTTT
    CCGAGGGCAACAAAATGCATTTCCGGCTCCACCGCAAGACGGGAGATTTGTTTGTGAAGGAGAAACTGGA
    TCGGGAGTCACTTTGTGGCAAAGCCGACCCTTGTGTTCTGCACTTTGAAGTAGTCCTGGTGGAGCCGCTG
    CAGTCCTTCCGGGCCGAGGTCAGGGTATTTGATATCAATGACAATGCCCCAGTTTTCCTAAACAAGGAGC
    CGCTTTTAAAGATTCCGGAGAGCACCCCTTTGGGTTCACGTTTTCCTCTGCAGAGCGCCCAGGATCTGGA
    CGTGGGCCTTAACGGTCTCCAGAACTACACCCTGAGTGCCAATGGGTATTTCCACCTGCACACCCGCTTC
    TGCAGCCACGGGCCTAAATATGCTGAGCTGGTGCTGAACAAACCCCTGGACCGAGAGGAGCAGCCTGAAG
    TCAACTTGACAATTACGGCGGTGGACGGCGGGTCCCCGCCTAAGTCTGGCACAGCTCACATCCACGTGGT
    GGTTCTGGATGTCAACGACCACGTGCCCCAGTTCTCGCGACTGGTGTACAGAGCCCAGGTATCAGAGAAC
    AGCCCCAATGGCTCTTTGGTGGCCACGGTGACTGCCGTGGACCTAGACGAGGGCACCAACAAAGCGATAA
    CTTACTCTTTAGCTCAAAACCCAGAAGCAATTCTCAAGACGTTTCAGATTGACCCTCAAAATGGAGAAGT
    TCGACTAAGAGGACCCCTCGATTTTGAAGCCATTGAAACATACGACATTGACATTCAAGCTACAGATGGT
    GGAGGCCTCTCTGCCCACAGCAAAGTCCTGGTAGAAGTGGTGGATGTGAATGACAATCCTCCCGAAGTGA
    TGGTCTCCTCTGTGTCCAGCCCACTCCCTGAAGACTCACCACCACAGACAGTAGTAGCCCTTTTCACTAT
    CAGAGACCGGGACATTCGAGTGGGAGGAAAAGTCACCTGCTTCCTCAGAGAAGACCTTCCCTTTGTAATC
    AAACCTACATTTGGGAATTCTTACTCACTGGTCACTGACAGAAGCTTGGATCGGGAGGAGGTCTCAGGCT
    ATAATATCACCATTGTTGCCATGGATACTGGACCACCTAGCTTGTCTGCCGAGACTATGATAGAGGTGCT
    AATATCCGACGTTAATGACAATCCTCCAATATTTCGGGAAGATTCCTATATCTTGACTGTTCGAGAAAAC
    AACAGTCCTGCGGTTTTTATTGGCAAAGTCCATGCTGAGGATCTTGATTTGGGTGAGAATGCCCAAATAA
    CATATTCTCTGTTGCCTCCAAAAAACGGAGATCTTTCAGTCTTTGCTTACATATCCATAAATTCAGGCAA
    TGGGAAGCTCTACGCGCTGAGAACCATGGATTATGAGGCCATTCAAGATTTTCAATTTGTGGTAAAGGCA
    ACTGATGGGGGCTTCCTGTCACTGAGTAGCCAAGTTACTGTCAGAGTGGTTGTCCTAGATGACAATGACA
    ATCGTCCAATGATCTTATACCCACTGCAGAACGGCACCTTGCCCTGCAATGACCTGGTGCCCAGGTCTGC
    AGAGGCAGGCTACCTAGTGACCAAAGTGGTGGCTGTGGATGGTGACTCAGGTCAGAATTCTTGGCTTTCA
    TATCATCTACTTAAGGCCACTGACCTTGGGTTATTTTCTGTTCAAAGACAAAATGGAGAAATCCATACAT
    TAAGGCAGATATCTGAGAGAGACCCCATGATGCAGAAATTGATCATTCTTGTTCAGGATCACGGCCAACC
    AGCTCTTTCCACTACTGTCTCACTCAACATCCTGCTGGTAGATGGCTTTTCAGAGCCCTACCTGCAGTTC
    CAGGATCCAACCAAGCATTCTAGAAAGGTAAATCCATCCACTAAATATTTGGTCATTTCTCTGGTCATCC
    TTTCCTTTCTCTTTCTCCTCTCTGTCATAGTGATCTTCATTATACATGTCTACCAAAAGATTAAATATAG
    AGAAAAGTTCACAATTCAAGAGCATTTCTATGATGACTGTAATTTCTCTAACAACCTGGTACAAGGACAA
    GGCAATGGATCCTTATCTCGGCCTTGTCCATATGAAATGTGTTCAGCCACTGGCACTGGTAATAGTGAGT
    TTCGCTTTCTTAAGCGTTTTATGCCCAACTTCCCTTTCCCTCATGCCACTGGGGAGATAAAAATGGAGGC
    TGGCTCCAGTTTGCCCCCAAATTCTGATAGGAATAAGTCTCAGAGATTAGAGGGCCATGACCAGGTATCT
    GATGACTATATGTA
  • Protein Sequence : Show Sequence
    >gi|14195607|ref|NP_037472.2| protocadherin beta-1 precursor [Homo sapiens]
    MAGTRRKSLQNRQVGSLLIFLCISVGDATTIRYSVAEEMESGSFVANVAKDLGLEVGKLAARGARLVSEG
    NKMHFRLHRKTGDLFVKEKLDRESLCGKADPCVLHFEVVLVEPLQSFRAEVRVFDINDNAPVFLNKEPLL
    KIPESTPLGSRFPLQSAQDLDVGLNGLQNYTLSANGYFHLHTRFCSHGPKYAELVLNKPLDREEQPEVNL
    TITAVDGGSPPKSGTAHIHVVVLDVNDHVPQFSRLVYRAQVSENSPNGSLVATVTAVDLDEGTNKAITYS
    LAQNPEAILKTFQIDPQNGEVRLRGPLDFEAIETYDIDIQATDGGGLSAHSKVLVEVVDVNDNPPEVMVS
    SVSSPLPEDSPPQTVVALFTIRDRDIRVGGKVTCFLREDLPFVIKPTFGNSYSLVTDRSLDREEVSGYNI
    TIVAMDTGPPSLSAETMIEVLISDVNDNPPIFREDSYILTVRENNSPAVFIGKVHAEDLDLGENAQITYS
    LLPPKNGDLSVFAYISINSGNGKLYALRTMDYEAIQDFQFVVKATDGGFLSLSSQVTVRVVVLDDNDNRP
    MILYPLQNGTLPCNDLVPRSAEAGYLVTKVVAVDGDSGQNSWLSYHLLKATDLGLFSVQRQNGEIHTLRQ
    ISERDPMMQKLIILVQDHGQPALSTTVSLNILLVDGFSEPYLQFQDPTKHSRKVNPSTKYLVISLVILSF
    LFLLSVIVIFIIHVYQKIKYREKFTIQEHFYDDCNFSNNLVQGQGNGSLSRPCPYEMCSATGTGNSEFRF
    LKRFMPNFPFPHATGEIKMEAGSSLPPNSDRNKSQRLEGHDQVSDDYM
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
35. PTCD1
  • Gene Name : PTCD1
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 26024
  • NCBI Protein GI : 38492358
  • Genbank Accession : AC004922
  • Protein Accession : NP_056360
  • Taxonomy ID : 9606
  • Chromosome No : 7
  • Gene Starting Position : 93650104
  • Gene Ending Position : 93673129
  • Gene Strand (Orientation) : -
  • Protein Name : pentatricopeptide repeat domain 1
  • Protein pI : 8.78
  • Protein Weight : 75381.12
  • Protein Length : 700
  • Protein Note : Also known as KIAA0632
  • DNA Sequence : Show Sequence
    >gi|157734172:93650104-93673129 Homo sapiens chromosome 7, alternate assembly HuRef, whole genome shotgun sequence
    TGAAACAGGGTCTTGCTCTGTTCCACAGGCTGGAGTGCAGTGGTGCAATGATGGCTCACTGCAGCCTCAA
    CCTTCTGGGCTCAAGGGATCCTCTCACCTCAACCTCCTGAGTAGCTGTGACTACAGGTGTGCACCACCAC
    ACCCAGCTAATTTTTAAATTTTTTGTGGAGATGGGCCCTTGGCCCAGGCTGGTTTCTTAACTCAAATGAT
    CCTCTGGCGTTGGCTTCCAAAAGTGCTGGGATTACAGGTGTGAGGCACCTTGCCTGGCCTAAGTACTTTT
    TTTTTTTGTTCCTCCTCCAAGGACTGTCCCGTTGCAATACTGAATGCCTTTTTTTTTTTGAGATGGAGTT
    TCGCTGTCACCCAGGCTGGAGTCCAGTGGCACGACCTCAGGTCACTACAACCTCCGCCTCCTGGGTTCAA
    GTGATTCTCCTGCCTCAGCCTCCTAAGTAGCTGGGATTACAGCTGCTACCACGCCCGAGTAATTTTTGTA
    TTTTTAGTAGAGACGGGGTTTTACCATGTTGGCCGGGCTGGTCCTGAACTCCTGACCTCAGGTGATCCGC
    CTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCGGCCTGAATGCTTTTTTTG
    ATCAAGGGTGGGGAAGGTTTTTAGACCATGGCCTGATGCTCTTTCCCCATCTTTTTGACAGAACTCTATG
    AATATTGTATTAAAGAAGGCTATGCAGACAAAAACCTGATTGCAAAATGGAAAAAGCAAGGATATGAGAA
    CTTGTGCTGCCTGCGGTGCATTCAGACACGGGACACCAACTTCGGGACGAACTGCATCTGCCGCGTGCCC
    AAAAGCAAGCTGGAAGTGGTAATGTCTGACACTCAAGCTTGGTGTTGTTTTCAGCTCAAAATTCTGCCTT
    AGTCGACTGCAAGGGATCTTATGTTATTTGGTTGGGCTGGAATGTCGTTTTGTCCCTGGATGTCCTGCTG
    GCTCTCCCTCGTGGGAGTGGGTCCCTGTATTCAGGAATCCATGTGAGGCAGCGTGTGGCTGTGTGTTTGT
    TAGGTCTGGGGTCAATCTCAACTCCACTTTTGGGCAAATTACTGAACCCCTTTCCTCACTTAGGAAATGG
    TGGTGGGGAGCCCTAATCCCAAGGTGGTGGCAGGGTGACATCAGGGAAGGACAACCAGTGAGTTCCTGTC
    CCTTTCAGTCCTCACAGTGATACTTTAACATTACCATCACTATCTTTCCCGCCCCCCCAAGACGGAGTCT
    TGCTTTGTCGCCCAGGCTGGAGTGCAGTGATGCCATCTTGGCTCACTGCAACCTCCACCTCCCGGGTTCA
    AGCGGTTCTCCTGCCTCATCCTCCTGAGTAGCTGGGACTACAGGCATGCACCACCACGCCTGGCTAATTT
    TGTATTTTTAGTAGAGACGGGGTTTCTCAATGTTGCTCAGGCTGGTCTCGAACTCCCGACCTCAGGTGAT
    CCACCCGCCTCAGCCTCCCAAAGTGCCGGGATTACAGGCATGAGCTACTGCGCCCAGCCCACTGTGTTTC
    TTCTTTCAGAACTTTCTGTTCCAAGTACCACTCCTAGGCCAGGCGCCTCAGGAGTGGTGCTGAGGGACAG
    GAGCTGAGCTCCATCCGGTTCCAAGCCCCTCTGAAAGGCAGCTGTGGCCCGAGGCAGAGTCACAGCAGTG
    GCCGCTCAGTGCAGCCCCTCAGCACAGTTGTTGGCCCCACCGCCCATCTGCCAGCCTGGCCTTCTTTTCC
    CCAGCTCCACACAGGGGCCTCTTCACATGAGTCCCCTTTATGCTGCAGTGAGACTGACAATGGAGAGCTG
    TGGCTGGATGCTGAGGACTTGGGTACTGAGCAGTGGGGGCGAGCAAGGGCCGCCTCCCCGACGGAAGACT
    GGGGTGTTGGTTTTCTGATGGAGGCAGGGACAGGTGGCAGAGTGGCAGAAGCCCCCTTCCTCTGAGTGCC
    CAGATTCCACAAGGAGTCCAGGTCAGGGGCTGGTGGGCTGGGGTGCTGCTTCAGCCCTGTGGCCAAAACA
    GCCCGCTGTAGACTTACCTGGGTTTCACCAGAGGATCCTGTTTACTTAAACTATGGTCTTGTTCTTCCAA
    AAAAAGAACTTGAAAAAGTAAAATAAACACAAGCCACTGTTCTAGGGCTTTCTGGCTGGCCAAGTGTTGG
    GCCGCACCAGGCCTGGGGGACAAGACCCTTCTTTCTGGGGGGCTCTGGGCCAGAAGGGTGTGACTCAAGT
    GCATAGTCTCCTGCCCTCTTCCCCCACCAGAGTGTACCAGCCCAGAGGCCCCCCTGAAGTCCCCAAACAG
    TAGCAGAGCCACGTCTGCTCATCGCAGGGTCTGTCATGCTCACTTAGCAGAATAACGAGACTCATTCACA
    CCGATTTCTTTTTCTTGATTGGCAAACGTGTGTTGGATTTGCAGAACATATTATAAATAGACATACAGAT
    GTAACCTCGGTGTCAACAGCAGCTGGTTCTACCTTCATGAGGGAGCCAAATTTTCCCTGTCCAGAGCTGT
    CAAGGAAGGGTTTCTGAGGTGTGTCCCTATATGGCATGGTGGCAGGTCCTTCGTGGGAGGGTTGCCACAT
    ATGTGAGTGTGCAGGGGCGAGCGTGGCGCAGTGGCATCGTCTCACTGTCCTCCTCCGTTGCAGGGCCGCA
    TCATCGAGTGCACACACTGTGGCTGTCGTGGCTGCTCTGGCTGAGGCTGGCGCGCTCCACCCTGGACTCT
    GGACTTCGCAGGTTCCTGCCTGTCACGCCACCCCCTTCCTGGGAGCAGCGAGCAGTGCCCCAGGCCCGAG
    TTGGAGCACGGTCTCTATGGGGAAGGCTTCGCTGTCTATCAGCTGTGATTTGTAAAAATAAAATCTTTAA
    ATCTCTCGAGCCCCACGTCTCTTCTTTCAGAGCATCGGCCTATGGAACCCGGCGGGGCGGCCCAGGCCCC
    AGGGACCAGATGCCCCAGCCCCCTTGTGGTGTGTGAGGTGACACACAAAGGTAGCTGGAGCTGGAAGTCC
    CGTGAAGGTGACACGCAAAGGTGGCTGGAGCTGCACTTGGACCTGCTGGGAGCACAGGCACCTTGGGCCT
    AGTGTGTGTCCTCACCAACACCTGTGACACGCTGCGGCTGTTCCTCAGGGCCTGGCTCTTCCCCCAGGCA
    GGAGGTGACACCAGCTCACTTGTCCTGGGGCTCCCACAGAGCACTGGGGGCCGAGCACATTGTTCCAGCT
    GTGCTCCCATCACCTGCCCCCAAGGGCACATCCGTCATCAGCCTCCTTGCCGGTGTCCTGGTCCCCCTGG
    GGCTTGGTCCGGAACTTCTGCCAGGGGTGCGGGGTTTCCTCTGCGGGCATCACTGTCAGCCACTGCTTGT
    AATAGGCTCGGAAGCCGTCAATCTTCTCCAGGTAGGTGTTCTTCCCTTGGTACTAGAATTAGAAAAGTGA
    GGCTAGAATCACTCCTGTTACCTACAGCTAATCCCAAACCTCGGCAGCTGGCTCAGGCCTCAGGGAAGCT
    GGTGGCTGCCATTTTCCCTTCCAGGTGGCTGCAGAGGACAGGGCAGAAAAGCTGTGAACACGCACTATGG
    GACTAGCTTACTTCAGAGAAACAGGTCCCCGCTGGGTACCCTGGGGCAGGCCCAGAGCCATCTGCCCAAT
    GGCGTTCTTCCCAAATAGGCACCACTGTGCTCCTCCATCCGCAGTTGCCACCCCAGGCCCCCAAGCCAGA
    ATCACTGGGTCCCACCAGCTCTGCCTCCACAGCCTGCCGAGCCCCTCCCCATCAAAGCCACCTCCCTCTG
    ACCATTATCTCAACACTGCCAAGATAGGCTCCATGAGATATGGGGAAAAAAGCAAAGACACACCAGACAC
    AAACTGAGAGCTGGTAGGGGAATATACCAAAAAAGCCAAAAAGTGCTATCCAACATACAGCTACTACCAC
    ACAATGACAAAAGGTTTGGCCGGGTGCGGTGGCTCACGTCTGTAATCCCAGCACTTTGAGAGGCCGAGGT
    GGGCAGATCACTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAGCATGGTGAAACTCCATCTCTACTAAA
    AATACTGGATGTGGCGGTGCATGCCTGTAGCCCCAGCTACCTGGGAGGTTAAGGCAGGAGAATGGCTTGA
    ACCCGGGAGGCAGAGGTTGCAGTGAGCTGAGATCACGCCATTGCACTCCAGCCTGGGCGACAGAGCAAGA
    CTCCATCTCAAAAAAAAACAAAAACAAAAAAAGGTTTGACTCAAAAGACTGGACAATTCTAAACCTTAAG
    GTGCCGGATAGAGCCTGAAAGCACAGAAAGCAAAACTCAACAGAATCACGAATATAAACAAGTCCACCAT
    CATGAGGGAATATGAGTACAGATGCTCCTGACAGCAGGGTTCCGCCCCCCAAAAACCCATCGTAAATCAA
    AAATCAATCTGGCTGTGTGTGCTGGCTCACTCCTATAATCCCAGTGCTTTGGGAAGCCGAGGCAGGCGGA
    TTACATGAGCTCAGGAGTTCCAGACCAGTTTGGGCAATATAGTGAAACCCTATCTAAAAGATTAGCTGGG
    TACAGTGGTACATGCCTGTAGTCCCAGCTCCTCAGGTGGCTAAGGTGGGAGGACTTCTTAAGCCTGGGAG
    GTCAAGGCTACAGTGAGCCATAATCGTGCCACTGCACTCCAGCCTGGGCAACAGAGTGATTCCCGTCTCT
    TTAAAAAAAAAAAAAAAAAAGAAAAAAGAAAATGCGGCACGGCATGGGGGCTCACAACTATAATCCCAGC
    ACTTTGGGAGGCTGGGGTGGGTGGATCACGAGGTCGGGAGTTCAAGACCAGCCTGGCCAAGATGGTGAAA
    CCCCGTCTCTACTAAAAAAAAAAAAAAAACCACAAAAATTAGCCTGGTGTGGTGGCAGGCTCCTGTAATC
    CCAGCAACTCGGGAGGCTGAGGCAGGAAAATCACTTGAACCTGGGCGGCAGAGGTTGCAGTGAGGCCAGA
    TCGCATCACTGCACTCCAGCCTGGGTGACAGAGTGAGACTCCATCTCATAAAAAACAAAAGGAAAATGCA
    TCTATTGCTGGCAACACAGCAGAGGGTCCTGACTTTATAATTATGCAAAAGTGATATACATTCAGTAGAA
    ACTAACCCCGCACAAAGTCGATCCTTACAGAGTTACATCCTGATAAAACCATTGCAAAGTTGAAAAATCC
    TGTGTAGAATCATCTTTAAGTCAGGGACATCTGTCTGTGACTTAAATATGATATGCTTTAGGTCTATGAT
    AGTCTTGTGACTGTCGAGTTGAACCCCTAGTGAGCCCTACGCCAGAGGGAAGAGACAATCCACTAGGAAG
    ATATGCACAACATGTGATCAACAAAAAACCAGTACCCAGAATATATAAAAAACCCTGGAATCAATGAGAA
    AAGGCTTGCAACCAACAGTTCAAAGCACAGACACAGTTCAAAAATGCCCAGCTATGCCTTCAGCCAGACA
    CAATTTCCATTGCTGTCCCACCTATTTGTAGGAAGCCCTCAAAATGGCATTCAGGCGTGACACCTGCACG
    GCGTTCCTGACCCCGCACCCTCTAGCTGTGTGTGTCCCTGCCCATTTACTTGTTTCCGCCCCATCCGGGC
    TGTGCTTCAAGCCATAGTGGCTCTCGATGCTCCGGAGATGCTCAGCACAGAGTGTGATTCGATGGACCCA
    AAAGCCAAACCCCAGAGCCTGATAGCCTCAGAACAGCAGGGAGAGGCTGTGGCCGTGACAAAGCAGATTC
    CATGACTCTGCCCTGCTCCTGCTGATACAGGAGGTTGAAAGAAATTATTTAGGCAGATAATAAGGGCAAC
    AGAGTCCTTGGCGAAATTTCCCTTTTAATAAAAAGCAGCCCCCAAATAATTTCTTTTCTAACAAAGAGCA
    GCCTGAAAAATCGAGCTGCAGACATAGATAAGCAAACTGGAAGCTTGCAGGGGTAAATGCTGGCGGCTGT
    GCCAACAGAAAAGGGCTACCTGGGAGCCAGATATGTTCAACGTGGGGGCCCCATCTTCCCTTCTTTGTTG
    CCATGTGTACAGTAAAGAACCAGGCAACATGGCGATGGCCACCTGTCTGCATAATAAAAGGTTAGGGTGG
    GGCACCAGCTTTTATACATGCTATGTAAATGGCACACCTGGTCCAACTAATCTTTTGCGTCCTCTGTAAA
    TCAGACACCACCTCCCCAAGCTCATCTACAAAACCTCTGCATTACACTATGGAAGTGGCAACCCACTTCT
    CTGGCACCTCTCTCTGCAAGGAGAGAGCTCTTCTCTTTTTTTTTTTTTCACCACCACACCGGGCGAGAGC
    TCTTCTTTCTTTCGCCTATTAAACTTCAGCTCTTAACCTCTTTTTTTTTTTTTTGAGACCAAGTCTCCAT
    CTATCGCCCAGGCTGGAGTGCAGTGGCGCGATCTTGGCTCACAACAAATCTCAGGTATTCACCCCAGACA
    ATGACACCACTTCACTGCCTGCCCAGTCCCTGAGCAAGCGACAACCTGTTTGGAGTACCCAGCAACGTGC
    AGGTGCTGGGGACCCAGATGAGCACAGCAGACAGCCTCTGCCTCCAGAAGCCTGATACAGGGAAGGCCAG
    GGCTGCCCATGAGCACATGATCCAAGACTGTAGGACACGGCATAAAGGAACAGAGTGGGAAGGGGGAGGG
    GAGACTTTCATCCCAGGTCAGAGCGGCAGCCAGCTGGGCATGTCTGAGGGCCACTTAAGCAGAGACCTCC
    AGGAAAAGTGGGTGCCACCTGAGGCAGCGGGAACACCAATCCCGAAGCAGGCAAGAGGTTGGTGGCCTCT
    GCGAACTGACAGAAGGCCACTGGGACAGACAGAGAAAGCAAACGCAAGGCTGAGACATGAGATGAGCCAC
    GCAGACAGGGACTCGCAGGCTGTGGTCTACAGACTTGGGTACTGCTCCTAAGGGAAGCTGCTGGACAGTT
    TCAAGCATGGCAACACAATCCCAAGGGCCAGAACCGGGGTTGGGTGGTGGGGGGAGGGGGTAGCAATGGT
    GAAGGAGCTGATGAGCTTTTGAGCTTGGGGCACACACCCGGTCAAAGGTGGGAGGGTACTGGGCTGCAAA
    CTCCAGCTGGCGGATGACCACTTCGTTCACCGGGACCCTGTTCTGCTTCATGTCCTTCAAGATGCTGATG
    AGATAGGTGTAGTTCAGCTTCCTGATGGCCGCGTTGATGAGGGCACTGTAGATGTGAGTGTTGGGGGTCA
    CCTGGGACTTCTAGAACACAGGGACATCGTAGAATCAAGATGATGGCAGCCATTGGGACCCAGCAGCTCC
    CACCCTCTGTCAATCAAGCTGGCTCCCGGGACCAGCGGCCAGGGCCAGCAAGGCAAACTTGGCAGCAACA
    ATGTGCTGAATATCCCTCTCCAGCAGGAAAGCCAAGCCCAGCCCCTCTGCTCAGCCCTAAAGAAGCTGCG
    GAAAAGGACGCTCGTGCTGCTCTCAGACACCGACTGACTCAGGCAGAAAATGATGTGGGAGACACCACCT
    CCTTGCTCCCTGGGGTGGGCTTCCTGTGACGCACGGTCACCAGGTGGTGGTTGGCCTAGAAAAGTGCAAG
    CCTTGGCTGGGTGAGCCCGGTAGGGTCCAGGAAAACAGCCAGAGGATGATGGACCTGGGGACACCGACAA
    CCCAGGACGCAGCCTCTGCTGTGGTCGGATGAAATCCACGCAGCACTTCCAATGGGGGTTAGGTGGGAGG
    CACAGACCTCGGGGGCTTTGAAAACTGCCCACGGCCCCTCAGGAATTCTGAGAATCCCTGCCATACCTCT
    AGAGTGTTGGGGCCGAGGCCTGTGCCCAGCCCTGTGTCAACCCACGGCCACCTTCTTCCGGATGGGAACA
    TCCGTGTGTCTCTACGCCCAGCCCTGAAGCACTCACTGGGGTCTGCTTCCCCCAAAATGGGACCAAAGAC
    CAGAAGGTCATTGACCCCACTCTCTTTGCCCCATGCACAGTTCATCCTCTCCAGGTGGGGGGTCTGCAGA
    CCCCTCAAACTTTCCTCCTGAGCTGCAGAGTGCTCCTGAGCACCATGGGTGTCCTGCCCAGGCCCGAGTC
    CACTCACCTTCATGTCTGTGAGAAGCTGTAGACCGTCCTTCGGCCTGTGGCACCCGATGGCCAGGTTGCA
    GAATGTCTGCAGGTTGGGGACGAGGCCCCTCTTTGCCAGGACCGGCAACAGCGCCTTGGCCCCCTCCAGG
    TCTCCCAGCTTGCTCTTCTTTCTCACCAGCGTGTTAAAGAATGTCAGGTCGGCCTCTACCTGGTGCTCAT
    CCAGGAGGGCCAGCAGCAAGGACTCTGCAGGACTCCCGGACTCCACCACCTCGGCCAGTAGCGTGAGGGT
    CCTGATGTCGGGCTGCTGCCTGTGCTCTGCCATCTTGCTCAGGAAGCCCTCCAGGCCCCCTATCAAGGCC
    AGCCGGTCAGCTGGGGTGGTCACCGTTCCAAAGGAGACCACTGTAGGGGGAACGGCCCCGGGGGTCAGGA
    GGTTGACTTCCAGCTCCACGGGAGGTGGCTTCAGGGCCACTGCGGTGAGGGCTGCTGTGTGGCTGGGCTC
    TGCCTTAGTATCCACCTCTGGTTGGGCCTTGCCGGGCACTCTGGCTTCCGGAGGCTCTGGAGGCCCAAGT
    GCCTGAGAAGGTTCCAGAAACAGCTGCCTCTCCAGGGCCTCCACATGCAGCATGGCTGACATGAGGTTGC
    CTGCCTTGGCCTGGGCTGTCCTCCTTGGCCGCTGCCTGCTCACTGGGGGCTGAAGCACAGTCGCCTCCTC
    CCTGGGCTTCAGAAGCAGCTCTGAGGCCACCTGGGGGTCCCCTAGGCCACAGTCCCGAGCTGCCACCAAC
    AGCAGGTTGTAGCTGTCCCGGCTCGGCTGTAGCCCTAGACTCAGCATCAGCCGCCACACCTGCCACGGAA
    GAGACAAACGTCAGCTGGGTGCTCCCATTCAGCAGCTGGGCGCAGGGCTCACGCCTGGAATCCCAGCACC
    TTGGGAGTTTTAGATGGGAGGATCCCTTGAGATCAGGAGTTCGAGACAAGACCGGGCAACATAGCAAGAC
    CCTGCCTCAAAAAAATACGCCAGGCGCGGTGGCTCACGCCTGTAATCCCAACACTTTAGGAAGCTAAGGC
    AGGCAGACTCCTTGAGATCAGGAGTTCGAGACCAGCCCGACCAACATGGTGAAACCCCATCTCTACTAAA
    AATACAAAAATTATTGGGTGTGGTATTGCATGCCCACAGTCCCAGCTACTCAGGAGGCTGAAGTGGGAGA
    ATCACTTGAACCCAGAGATCGAGGTTGCAGTGAGCCGTGATCATGCCACAGCACTCCAGCCTGGGTGACA
    GAGCGAGAGTGTCTCAAAACAACTGTAAAACATTTAAAAAATTGGCCGGGCCTCTCCCTCTCCCTCTCCC
    TCTCCCCCTCCCTCTCCCCACGGTCTCCCTCTCCCTCTTTCCACAGTCTCCCTCTGATGCCCAGCTGAAG
    CTGGACTGTAACTGCTGCCATCTCGGCTCACTGCAACCTCCCTGCCTGATTCTCCTGCCTCAGCCTGCCG
    AGTACCTGCGATTGCAGGCGCGCGCCACCACGCCTGACTGGTTTTTGTATTATTTTGGTGGAGACGGGGT
    TTCGCTGTGTTGGCCAGGCTGGTCTCCAGCTCCTAACCGCGAGTGATCCACCAACCTCGGCCTCCCGAGG
    TGCCGGGATTGCAGACGGAGTCTCGTTCACTCAGTGCTCAATGGTGCCCAGGCTGGAGTGCAGTGGCGTG
    ATCTCGGCTCGCTACAACCTCCACCTCCCAGCCGCCTGCCTTGGCCTCCCAAAGTGCCAAGACGGCCGCC
    ACCCCGTCTGGGAAGTGAGGAGCGTCTCTGCCTGGCCGCCCATCGTCTGGGATGTGAGGAGCCCCTCTGC
    CTGGCTGCCCAGTCTGGAAAGTGAGGAGCGTCTCTGCCCAGCCGCCATCCCATCTAGGAAGTGAGGAGCG
    CCTCTTCCCGGCCGCCATCCCATCTAGGAAGTGAGGAGCATCTCTGCCCGGCCGCCCATCGTCTGAGATG
    TGGGGAGCGCCTCTGCCCCGCCGCCCCGTCTGGGATGTGAGGAGCGCCTCTGCCCGGCCGCGACCCCGTC
    TGGGAGGTGAGGAGCGTCTCTGCCCGGCCGCCCCGTCTGAGAAATGAGGAGACCCTCTGCCTGGCAACCG
    CCCCATCTGAGAAGTGAGGAGCCCCTCCGCCTGGCAGCCGCCCCGTCTGAGAAGTGAGGAGCCCCTCCGC
    CCGGGAGTCACCCTGTCTGGGAAGTGAGGAGCGTCTCCGCCCGGCAGCCACCCCGTCCGGGAGGGAGGTG
    GGGGTCAGCCCCCGCCAGGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGTCAGCCCCCCACCCGGCCAG
    CCGCCCCGTCCGGGAGGGAGGTGGGGGTGGTCAGCCCCCTGCCCGGCCAGCCGCCCCGTCCGGGAGGTGA
    GGGGCACCTCTGCCCGGCTGCCCCTACTGGGAAGTGAGGAGCCCCTCTGCCCGGCTAGCACCCCGTCCAG
    GAGGGAGGTGGGGGGGTCAGCCCCCCGCCCGGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGGTCAGCC
    CCCCGCCCGGCCAGCCGCCCCGTCCGGGAGGTGAGGGGCGCCTCTGCCCGGCCGCCCCTACTGGGAAGTG
    AGGAGCCCCTCTGCCAGGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGGGGTCAGCCCCCCGCCCAGCC
    AGCCGCCCCGTCCGGGAGGTGAGCGGCGCCTCTGCCCGGCCGCCCCTGCTGGGAAGTGAGGAGCCCCTCT
    GCCCGGCCACCACCCCGTCTGGGAGGTGTGCCCAGCGGCTCATTGAGAACGGGCCATGATGACAGTGGCG
    GTTTTGTGGAATAGAAAGGGGGGAAAGGTGGGGAAAAGATTGAGAAATCGGATGGTTGCCGTGTCTGTGT
    AGAAAGAAGTAGACATGGGAGACTTTTCATTTTGTTCTGTACTAAGAAAAATTCTTCTGCCTTGGGATCC
    TGTTGATCTGTGACCTTACCCCCAACCCTGTGCTCTCTGAAACATGTGCTGTGTCCACTCAGGGTTAAAT
    GGATTAAGGGTGGTGCAAGATGTGCTTTGTTAAACAGATGCTTGAAGGCAGCATGCTCCTTAAGAGTCAT
    CACCACTCCCTAATCTCAAGTACCCAGGGGCACAAACACTGCAGAAGGCCGCAGGGTCGTCTGCCTAGGA
    AAACCAGAGACCTTTGTTCACTTGTTTATCTGCTGACCTTCCCTCCACTATTGTCCTATGACCCTGCCAA
    ATCTCCCTCTGCAAGAAACACCCAAGAATGATCAATAAAAAAAAAAAAAAAATTGGCCGGGCGCGGTGGC
    TCACGCCTGTAATACTAGCATTTTGGGAGAGCAAGGCGCCGGATCACGAGGTAGGAGAGCGAGACCATCC
    AGGCTAACATGGTGAAACTCTGTCTCTACTAAAAATACAAAAACAAAATTAGCTGGGCATGGTGGTGGGC
    GCCTATAGTCCCAGCTACTCAGGAGGCTGAGACGGGAGAATGGTGTGAACCCAGGAGGTGGAGCTTGCAG
    TGAGCCGAGATCATGCCACTGTACTCCAGCCTGGGCGACAGAGCAAGACTCCATCTCAAAAAAAAAAAAA
    AAAAATTAAAAACATTAAAAAGAAAAAGAAAAAAAAAGCTCTCCAGCGGGGAGCTGTGGTTCACACCTGT
    AATCCCAGCACTTTGGGAGGCCCAGGCGGGCAGATCACATGAGGCCAGGAGTTCAAGACCAGCCTGGTCA
    ACATGGTGAAACCCCGTCTCTACTAAAAATACAAAATTAGCTGGGCGTGGTGGCGGGCGCCTGTAATCTC
    AACTACTCGGGAGGCTGAGGCAGGAGTATCGCGTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATC
    GCTCACTCCAGCCTGGGCAAGAGTGAGACTCCATCTCAAAATAAATGAATGAATGAATGAATGAATGACT
    CCTGGGTTGCACCTGCAGAAGTGCTAATGGGGGTGTGGGAGGGTTGAACAAGTCCCCAGGTGAGTGTGTG
    GCAGGCTTTCAAGGAAGGTGAGTTTAGAGGGGCTAAGCCGACTGCAAAGCAGGGTGGGAGGGGCGGGGGC
    CACCCTCATTCCATCTCCAAGGGCCTCCTCTCCCTGCCATCTGTAGAATGGAGGCTACAGTGGCTGCTGG
    GTTTTCAGAAAAGAGCACAGATATAAAAATACTCAGCACAGGGCCATCGTGGGTGCTCACAGGATCACCA
    TTTTGCACCTCTGGCACCGGGGAAGCAGGGCAGGAAGGTGGGGTGGGAGGGACATACCTGGAGGGCGTAC
    CGGAAGCCTGTCTTCTTGTCTTGGATGCAGCCCATGAGCAGGAAACTGAAGGTCTCCTCTGTGACCACGT
    GCCCTTTGTGGATGATTTCCTGGGGGAGGGAACAAAGACGTCCCACTGAGAACCTGCAGCAGGCTGGGCA
    TGGTAGCTCATGTCTGTAATCCTGGCACATTGGGAGGCCAAGGCAGGATGATCACTTGAGGCCAGGAGTT
    TTGTGACCAGCCTGGGCAACATAGCAAGACCCTGTCTCTAGGAAGCCTTCCCTCGAGTGCACAAAGCAGG
    ACACAGCACACGGTGGCATACACAATCTCCAGTAAGGTCTCATCAGATCCATCTGTGAACCCCAGGGCCC
    AATACCGGCTGTCTCATCCTCCCTAAGCCTTCCTACATGAGGAGAGACAGACACCACAGCCCTGCCCCTG
    ACACGTGGGACCAGCCCCATGAAGGGGAGCAGGACGGCAGGGGAAGCCCCACCTTGAACACATCGAGGCA
    CATCCTAAGGTCTGCGCACTTGGCAGCCATCTTCAGCAGCGCGTGGTATGTTTTCAAGTTGAGCTCGAAG
    TTTTTGGCCTGCAGCTGCTGCCGGAGCTTCAGGGCGCTCTGTAGAGCTGAGTCCTTCCAGGGGGACTCGG
    CACAGACGTTGAACAGGGCCGTGTAGGTGGCGTCCGAGGGCTCCAGGTCCCGCTTTTTCATCTGTGGAGA
    TGGAAGAAGCTCAGTGGTGGCCCGGGATCAGCTGGACGCACAGGTGAGCAGCTGCCCCAGAGGAGGCTGA
    GGCTGGAGAGGACCCCGTCACTGCATCCACCAGGCCAGGCTCTAAGGCAGAGCCATGGACACATCAGAGC
    CCAGCTCTCCAAACTCAGACACATTGAAGGGACTCTGAAAACTGAGCCCACACACCCAGATGTACCAAAC
    AGGAGCTACCCTGCCCTGGGCACTTGTCAGCCCACTCCAGGCCTGGGGTCCTGGCAGCAAGGTCTTCCTG
    ATGTCCCCACCTTCAGGCCACCCCGTCAAGGGGAAAGGGCCTGTAGTGCTTACTACCTGAGGGCTCCCGG
    GCAAGTTAGCACTTCCTCGCGAAAATGGGGATGACACCATCACCTTGAAACCAGGCATGCCTGTGGCAAG
    TGGGAGGATGCACTGCAGTGCTCTAGGGGTGGCCCCGAACCAGTGGCACAATGCACATTCTCAGGGCCAC
    CTGATCTGGTAAGTCACAAGCCCCGGAGCTGAGTGACACTCAGCTTACTGAGCCTCCAGGGGACTAAGGC
    ATGTGCTCAGGGTTGACAATCCGTGGTGAAGCCTAGCACTCGCTATTGGCCAGAGAAGGGCCATCTGGAC
    AAAGGCAGGCGATAGGCAGCTCAGCGGCAGAAAGAACCCTGCTAGAGTCAGGCCATCTGGGGCTCAAATC
    CCAGCCCCGGTGCATTCAAGTGGGGTGGCCTTGGACATGTCCCTTCATCTCTCTGTGCCTCAGTTTCCTT
    ATCTGAAAAGCAAAGAGGGCAGTCCCTACTTTGAGGATGATGTAAGCTCTGCGTGTATTGCTGCACTCCA
    CAGAGGTGCACTGACAACTCACTACCTGCCCAGCAGCAGCCCTGGGGTTAGCAGAGCGGGCACAGCCTAT
    CCTTACTGAGCTTACACTCAAGAAGGGAGACACACTGCAGGTGAGTCACCAAAAACAGTAACAAGGCTGG
    GTGCCGTGGCTCACGCCTATAATCCCAGCACTTTGGAAGGCTGAGGTGGGCAGATCACCTGAGGTCAGGA
    GTTCGAGACCAGCCTGGCCAACATGGCGAAACCCCGTCTTCTACAAAAAACACAAAAAAATTAGCCAGGC
    ATGGTGGTGGGCGCCTTTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAACCTGGGAGG
    TGGAGGTTGCATTGAGCCGAGGTCGCACCACTGCACTCCAGCCAGGGTGACAGAGCCAAAACTCCCGTCT
    CAAAAAAATAAATAAATAAAATAAAATAAATAAAAACAATAAAAATATTAGTTTTTTTTCTTTTTTTTTG
    AGATGGAGTCTTACTCTGTCGCCAAAAAAAAGGACTGTAGGAAAAAGAAAGAGAGATCAGACTGTTACTG
    TGTCTATGTAGAAAGAAGTAGACACAAGAAACTCCATTTTGTTCTGTACTAAGAGAAATTCTTCTGCCTT
    GAGATGCTGTTAAACTGTAACCCTAGCCCCAACCCTGTGCTTGCAGAGACATGAGCTGTGTTGACTCAAG
    GTTTAATGGATTTAGGGCTGTGCAGGGTGTGCTTTGTTAAAAAAGTGCTTGAAGGCAGTAGGCTTGATAA
    AAGTCATCGCCATTCTCCAATCTCGAGTACCCAGGGACACAATACACTGCGGAAGGCCGCAGGGACCTCT
    GCCCAGGAAAGCCAGGTATTGTCCAAGGTTTCTCCCCATGTGATAGCCTGAGATATGGCCTCGTGGGAAG
    GGAAAGACCTGACTGTCTCCCAGCCTGTCCCCCAGCCCGACGCCCATAAAGGGTCTGTGCTGAGGAGGGT
    TAGTGAAAGAGGAAGGCCTCTTTGCAGTTGAGATAAGAGGAAGGCATCTGTCTCCTGCTCGTCCCTGGGA
    ATGGAATGTCTTGGGTGTAAAACCAGATTGTGTGTTCTATTTACTGAGATAGGCGAAAACCGCCTCAGGG
    CTGGAGGTGGGACCTGCTGGAGACAATACTGCTCTTTGATGNNNNNNNNNNNNNNNNNNNNAAATGGGGA
    TGACACCATCACCTTGAAACCAGGCATGCCTGTGGCAAGTGGTAGGATGCACTGCAGTGCTCTAGGGGTG
    GCCCCGAACCAGTGGCACAATGCACATTCTCAGGGCCACCTGATCTGGTAAGTCACAAGCCCCGGAGCTG
    AGTGACACTCAGCTTACTGAGCCTCCAGGGGACTAAGGCATGTGCTCAGGGTTGACAATCCGTGGTGAAG
    CCTAGCACTCGCTATTGGCCAGAGAAGGGCCATCTGGACAAAGGCAGGCGATAGGCAGCTCAGCGGCAGA
    AAGAACCCTGCTAGAGTCAGGCCATCTGGGGCTCAAATCCCAGCCCCGGTGCATTCAAGTGGGGTGGCCT
    TGGACATGTCCCTTCATCTCTCTGTGCCTCAGTTTCCTTATCTGAAAAGCAAAGAGGGCAGTCCCTACTT
    TGAGGATGATGTAAGCTCTGCGTGTATTGCTGCACTCCACAGAGGTGCACTGACAACTCACTACCTGCCC
    AGCAGCAGCCCTGGGGTTAGCAGAGCGGGCACAGCCTATCCTTACTGAGCTTACACTCAAGAAGGGAGAC
    ACACTGCAGGTGAGTCACCAAAAACAGTAACAAGGCTGGGTGCCGTGGCTCACGCCTATAATCCCAGCAC
    TTTGGAAGGCTGAGGTGGGCAGATCACCTGAGGTCAGGAGTTCGAGCCCAGCGTGGCCCACATGGCGAAA
    CTCTGTCTACTAAAAATACAAACAAAATGAGCCAGGCATGGTGGCATGTGCGCCTGTAGTCCCAGCTACT
    CGGGAGGCTGAGGTATGAGAACTGCTCGAGCCCAGGAGGCGGAGGTTCCAGTGAGCCGAGGTCGCACCAC
    TGCACTCCAGCCTGGGCGACAGAGCGAGACTCTGTCTCCAAAAAAAAAGGACTGTAGGAAAAAGAAAGAG
    AGATCAGACTGTTACTGTGTCTATGTAGAAAGAAGTAGACACAAGAAACTCCATTTTGTTCTGTACTAAG
    AGAAATTCTTCTGCCTTGAGATGCTGTTAAACTGTAACCCTAGCCCCAACCCTGTGCTTGCAGAGACATG
    AGCTGTGTTGACTCAAGGTTTAATGGATTTAGGGCTGTGCAGGGTGTGCTTTGTTAAAAAAGTGCTTGAA
    GGCAGTAGGCTTGATAAAAGTCATCGCCATTCTCCAATCTCGAGTACCCAGGGACACAATACACTGCGGA
    AGGCCGCAGGGACCTCTGCCCAGGAAAGCCAGGTATTGTCCAAGGTTTCTCCCCATGTGATAGCCTGAGA
    TATGGCCTCGTGGGAAGGGAAAGACCTGACTGTCTCCCAGCCTGTCCCCCAGCCCGACGCCCATAAAGGG
    TCTGTGCTGAGGAGGGTTAGTGAAAGAGGAAGGCCTCTTTGCAGTTGAGATAAGAGGAAGGCATCTGTCT
    CCTGCTCGTCCCTGGGAATGGAATGTCTTGGGTGTAAAACCAGATTGTGTGTTCTATTTACTGAGATAGG
    CGAAAACCGCCTCAGGGCTGGAGGTGGGACCTGCTGGAGACAATACTGCTCTTTGATGCACGAGATGTTG
    TGTAGGTGCACATCAAAGCACAGCACCCTTCCTTAAACTTATTTATGACACAGAGACCTTTGCTCACATA
    TTTTCCTGCTGACCCTCTCCCCATTACCCTATCGTCCTGCCACATCCCCCTCTCCGAGATGGTAGAGATA
    GTGATCAATAAATACTGAGGGAACTCAAGAGACCAGTGCCTGCGCAGCGCAGGTCCTCCGTATGCGCAGG
    TCCTCCGTATGCGGGATGCTGAGCGTATGCTGAGCACCGGTCCCAGGGGACCACTGTTCTTTCTCTATAC
    TTTGTCTCTGTGTCTTATTTCTTTTCTCAGTCTCTCGTCCCACCTGACGAGAAACGCCCACAGGTGTGGA
    GGGGCTGGCCCCCTTCAGAGGAGCAGATGCGGTGGCTGGGCTGTGACCTGTAATCCCAGCACTTTGGGAG
    GCTGAGGCGGATGGCCAGGGGTTAGAGACCAGCCTAGACAAAGCAGCACAATCCCACCTCTACAAAAAAC
    TTAAAAATTAGCCAGATATGGTGCTACACACCTGTGCTCACAGCTACTGGCGAGGCTGAGGCAGGAGGAA
    CTCTTGAGCCAAGGAGTTGGAGGCTGCAGTGAGCTATGACTGCACTCCAGCCTAGGTGACAGAGTGAGGC
    TCCCTCTGTTTTTTTTTGAAGGGAAAAGAAAGGGAACATGGGGACCGCCACAGTCCAGGCAGGTGCTAAA
    GGGCTTTCCATGTCATCTCGTTTATCACCCACAATCACCACCATTTTAAGGAGATGACTCTGAGGCCAGG
    GAGGTGAAGTCACCTGCCCAGTGTAAAGCACTAGCAAGTGGCACACTTGGGATCCGCCCCCAGCTTTTCA
    GAGCCTCACCCCGGCTGCCCTGCGCCCACATGCACCTGGTTGTAGAGGTTGAAGGCCTTCTTCAGGTAGC
    CAACCCGCCCGCAGCCCCCAATCAGCACCGTGTAGTTGCTCTCCATGGGCTGCAATCGCTCCTCCTTCAG
    CATCTGCCTCTCAAACAGGTCCAGGGCTTCAACCAGCTGCAGGGAAGAGGCAACAGGGCAGGGGCTCAGA
    ATGGCCCCAGAGACCCTCAGCAGAGAGAGGGAGGTTGAAGCTGAGGGACCCTCCTAAAATGGTGGAGAAC
    GGCGGCCCTGGGTGACTGCAAACCCCCTGGGCCCCCAGCTGTTTCTGCCCCATCCCTCACCTCACACCAT
    TTCCCCGGTGAAGCCTCAGTTCATTCCTTTATTCCTTCATTATTTTCATCTGTTCATTCAAACAATAATT
    ACTGAGTGCTTCAATGAGCCAGACAAGGTCTATCCACTGCATATACGGCTGGGAACAAGAGAGAGCTCCT
    TCTTGGAGCTCATGTTTTGGTGTGGAAAGTCAGACATGAGGCCAGGCGCAGTGGCTCACGCCTGTAATCC
    CAGCACTTTGGGAGGCTGAGGCGGGCAGATCACAAGGTCAGGAATTCGAGACCAGCCTGGCCAACATGGT
    GAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGCACACACCTGTAATCCCAGCTAC
    TTGGAAGGCTAAGGCAGAAGAATTGCTTGAACCCGGGAGGCAGAGGCTACGGTGAGCCAAGATTTCACCA
    CTGCACTCCAGCCTGGGTGACGGTACAAGACTCCATCTCAAAAAAAAAAAAAAAACAAAAAAACAAACCA
    GACAGACATGAAATGGGATAATTAAGAAAGACACACAGTATATCAAGTGATGACAGGTGGTACGAACAAA
    ACTAAAGCAGCGGCCAGGTGTAATGGCTCATGTTTGTAGTCCCAGCATTTCGGGAGACCAAGGCGGGCGG
    ATCACCTGAGGTCTGGAGTTTGAGACCAGCCTGGCCATCATGGAGAAACCCCATCTCTACTAAAAATACA
    AAAATTGGCCAGGTGTGGTGGCACATGCCTGTAATCCCAGCTACTCAGGATGTTGAGATGGGAGAATTGC
    TTGAATTCCCCAGGAGGCGGAGGCTGCAGTGAGCCAAGATCGTGCCACTGCACTCCAGCCTGGGCGACAG
    AGTGAGATCCTGTTATAAAAAAAACCTAAAGCAGGGAGTGCTAGAAGGGTGTGGAGGATTCACTTTTCTT
    TTTCCTTTTTTTTTTTTTGAGACAGACTCTTGTTCTGTGACTCAGGCTGGAGTCTAGTGGTAAGATCAGC
    TCAGTGCAACCTCCGTCTCTACAAAAAAAAAAAAATACAATAAAATAAAACTGCGGTGGAGGATGGCAAA
    GGGTTGCGATACTTACAGCCATAAGGCTGGCAGTTCAACAGGGTAGGCCCAAAACTTAGAGGCTGGGAAA
    GTCAGGTGACCCCTTGCAAAACACTGAGCCACCAGGGGAAAGAAGCCAGGAGCCACAGAACCCAAAGTGC
    CCCTTACCTTCCCTTCCTTGATCAGGTGTTTGCACTGCAAGAAGTACCAGTACGGGGTGTTTCTCCGGCC
    TCGCCATAATTTGGGCTCCGGTTCCATTTGCTCATCTCTCCGTTCCCCAAACCGCAGGTTATGGAACTGG
    GCTGCGGATTTGCGGAATAGTCTCCGGGAGGAGTATTTGTCAGAGAGGGTCCCAAAACTCTCCTCCTCCT
    CCTCGTCTTCTTCCTGCGTGGCCGTGGAGTTGGAGTGGCTCGGGTCAGAGCCCAGGCTGCCCGTGTTTTC
    CTGACGCTCCTGGCCGAGGGGCAGCTGAGAGGAGGAGCTGCTGAAGGGCGCCCACATTGGCCGCATCAGC
    CCCTCCCTGCCTCCTGCCCACCTGGCTCTACAGGGGTCCAGGTGTTGCAGGATGAACAGTCCCATGGGGC
    GGGCCCTGGCGAACAGTCGAGCGAGTCTCACGAAGTCCATTTCTGGCTTTCCTGTCCCTCCAGGGCCTGG
    AATATATCAGGAAAAATAAGAGAGTCAACTCAGTTCCTAGTAACAAAAGGCAGAAAGAAAGAAGTGGTTA
    CAAGTATGGGCCCAGGCCAGGCGCGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGTCGAGGCTGG
    CATATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGACAACATGGTGAAACCCCGTCTCTATGAAAAAT
    ACAAAAATTAGCCAGGCTTGGTGCTGTGCGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAAT
    CACCTGAACCTGGGAGATGGAGCTTGCAGTGAGCTGAGATTGCGCCACCATACTCCAACCTGGGTGACAG
    AGTGAGACTCTGTCTCGAAAAAAAAAAGAAAAGAAAGAAAGAAAAAGAACAGGCCAGGGGCGGTGGCTCA
    TGCCTGCACTCCCAGCACTTTGGGAGGCCGAGGCGGGCAGATCAGTTCAAGACCAGCCTGGCGACACCCC
    ATCTCTACTAAAAATACAAAAAACATGAGCTGGGTGTGGTGCTACACGTCTAATCCCAGCTACTGAGGAG
    ACTGAGGTAGGAGAATCGCTTGAACCCAGGAGACGGAGCTTGCAGTGAGCTGAGATCGCGCCACTGCACT
    CCAGCCTGAGTGACAGTGAGACTCTGTCTCAGAAAAAAAAAAAAATAATAATAATAAATAAAATAAGAAG
    TATGGGCCCAGAGGTTAGAAAGCCTAGGTTCAGGTCAGACATAGCTCACTGCAGCCCCACCCTCCTGTGC
    TCAAGCGATCCTCCCATTTCAGCCTCCCGAGTAGCTGGAACTACAGGTGTGAGCCACTATGCCTGGCTAA
    TTTTATTTTTTGGTAGAGACAGGGTCTTGCTATGTGCCCAGGCTGGTCTTGAACTCCTGGACTCAAGCAA
    TCCTCTCGCCTCGACCTCCCAAAGCACTGGGATTACAGCAATGAGCCACCATGCCCAGCCTAAAGTATTT
    TAGAGTCTGGTTATATTAGAAGGTTGCGATTGGAAAAGTCTTACAAGTGATGGCAGATTGAACAGATTAA
    AAACTAGGCCCAGTAGGTCAGGAGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGG
    GTGGATCCCCTCAGGTCAGGAGTTCGAGACCAGCCTGACGTGGTGAACCCTATCTCTGCCGAAAATATAA
    AAATTAGCTGGGCGTGACAGCGTGCCCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACT
    TGAACGTGGGAGGCAGAGGTTGCAGTGAGCCAAGATCGCGCCATTGCACTCCAGCCTGAGCGACAGAGCA
    AGACTCCATCTCAAAACAACAACAACAACAACAAACAAAAAACTAGGCCCAGCAAGGGGAGAGACTTGCT
    GGGGTCCTGCAGCAGTGCAGTGGGTGAGACTGGCCAGCTTGGGCTGGGCTGGCTTTGAACTCAGGTCCAT
    GTAACGCATTGATGATTGTCCTGACCAGCACCTTTTGTGAAACTAGTGCAGGGGGCCTTTATCATTCTGC
    ATCTGGCAAGCCCTGCCACTCCTTCTTACAAGCATGGTGTTTATGTAATTTTCTTTTTTGTGGAATGGGG
    TCTTCATGTTGCCCAGGCTGGAGTGCGATGGCACAGTGGAGTGCAAATGCACAATCACTGCAACCTCCAA
    CTCCTGGGCTCAAGTGATCCTCCTACCTCAGCCTCTCAATTAACTAGCACTACTGGTGCACACCACCATA
    CCTGTCTAATTTTTTGTATTTTTTTGTAGAGATGGAGTCTCCCTATGTTGCCAGGCTGGTCTAGAACTCC
    TGGCCTCAAGCAATCCTCCAGCCTTGGCCTCCCAAAGTGTTGGGATTGCAGGCGTAAGCCTCCATGCCCT
    GCCTTACGTGCATTAATTACAATACACAGAATTACAAAACAAAAAGCAATGATATTGAAATGGCATCAAA
    ATGCTTTAAAAACAAATTTCTGCTATACTAGTATAGATGCTTATTTTCAATGCATGAAATAACAAGACCC
    TATCATAATTTCTTTTCTTTTCTTTTTTTTTTTTTGAGACAGTCTCTCACTCTGTCCTCAGGCGTGACCT
    CGGCTCACTGCAATTTCCGCCTCCCGGATTCAAGCGATTCTCCTGCCTCAGCCTCCCCTGGAGCTGGGAC
    TACAGACGCATGCCACCAAGCCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCATGTTGGC
    CAGGATGGTCTGGATCTTTTGACCTTGTGATCCGCCCGCCTCGGCCTCCCGAAGTGCTGGGATTACAGCC
    GTGAGCCACCGCGCCCGGCCGACTCTATCATAATTTCAAAGCAGTAATAAATGTAAGTGACAGCTTAAGA
    TACCACCTTGTAGTTGCTGCACAAACTGGGTTTTTTTGCGTCTCACTCTGTCACCAGGCTGGAGTGGGGT
    GGTACAATCTCGGCTCAATGCAACCTCTGCCTCCCGGGTTTAAGCCATGCTCATGCCTCAGCCTCCCAAG
    TAGCTGGGATTACAGGAACGCGCCACTACTCCAGGCCAATTTTTATATTTTCAGTAGGGACGGGGTTTTG
    CCATCCTGTCCAGGCTGGTCTCGAACTCCTGACCTCACGTGATCCTCTCGCCTCAGACTCCCAAAGTGCT
    GAGGTTACAGGTGTGAGCCACCATGCCTGGCCCTGCACAAACTGTTATCTGGAACTATCATTTACACTCA
    CCCTCACAGTTTCAGGCACTGCTAAGGCTGCTCCAGCAATCAGGAAAGGAAATGCTATACTTCACATAGA
    GAAAAATAGGGGTGCATTTTGTTCCTACACAAGCTCATTACCCCACCCTGGTTAAAAAAAACCCTGAATT
    AGGCTGTATAGTGGCTCATGCCTGCAATTCCAGCACTTTGGGAGGCCCAGGCAGGAGGATCGCTTTGGGC
    TTGGGGCCAGGAGTTCGAGACCAGCCTGGGCAACATAGCAAGACCCGCCACCCGCCCCCCCAACCCCGCC
    GTCTCTACAAAAATTAAAAATAAAATTTTAAAAATTAGCCAAGTGTGGTGGTGCAGCGTCTGTAGTTCCA
    GCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGTCTGAGAAGTTGACTGCAGTGAGCTATGATCGTA
    CCCCTGTACTCCAGCCTGGGCCACAGAGCGAGAAAAAACCCTGAATTAGAGACACGCTGCGGCCGTCCTT
    CCTCGGAGAGACCCGCCCCTCCTCAGAGGCCCACCCGGACCCTCAGGCCAACGCCGACCCCTCTCCACAC
    GGACTTCTGCAGCCTCAGTTTCCCTCCTCAGACGCCCCCGGCTCCAATCCCGGCTCGGGCACTTCCTTCC
    CCTCTGGCGTGTCTTGGCCCGGGTCCCGGGGCTCCTGCGAGGATCACACAGGAACTCACTTGAAGTGTCC
    GGCGCAGTGCACTCCGACGGGGAGCCCTGCCCGGTCCCCGCGGCGAACCAGTCTCTTCCTCGGGTCCCCC
    TCTCCCCAAGCGCGCAGGCGCAATCCGCGTCGCCGACTCACGCGGCTGGTTTCAGGTTTCTGGCGA
  • Protein Sequence : Show Sequence
    >gi|38492358|ref|NP_056360.2| pentatricopeptide repeat-containing protein 1 [Homo sapiens]
    MDFVRLARLFARARPMGLFILQHLDPCRARWAGGREGLMRPMWAPFSSSSSQLPLGQERQENTGSLGSDP
    SHSNSTATQEEDEEEEESFGTLSDKYSSRRLFRKSAAQFHNLRFGERRDEQMEPEPKLWRGRRNTPYWYF
    LQCKHLIKEGKLVEALDLFERQMLKEERLQPMESNYTVLIGGCGRVGYLKKAFNLYNQMKKRDLEPSDAT
    YTALFNVCAESPWKDSALQSALKLRQQLQAKNFELNLKTYHALLKMAAKCADLRMCLDVFKEIIHKGHVV
    TEETFSFLLMGCIQDKKTGFRYALQVWRLMLSLGLQPSRDSYNLLLVAARDCGLGDPQVASELLLKPREE
    ATVLQPPVSRQRPRRTAQAKAGNLMSAMLHVEALERQLFLEPSQALGPPEPPEARVPGKAQPEVDTKAEP
    SHTAALTAVALKPPPVELEVNLLTPGAVPPTVVSFGTVTTPADRLALIGGLEGFLSKMAEHRQQPDIRTL
    TLLAEVVESGSPAESLLLALLDEHQVEADLTFFNTLVRKKSKLGDLEGAKALLPVLAKRGLVPNLQTFCN
    LAIGCHRPKDGLQLLTDMKKSQVTPNTHIYSALINAAIRKLNYTYLISILKDMKQNRVPVNEVVIRQLEF
    AAQYPPTFDRYQGKNTYLEKIDGFRAYYKQWLTVMPAEETPHPWQKFRTKPQGDQDTGKEADDGCALGGR
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
36. SLC11A1
  • Gene Name : SLC11A1
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 6556
  • NCBI Protein GI : 109255241
  • Genbank Accession : AC021016
  • Protein Accession : NP_000569
  • Taxonomy ID : 9606
  • Chromosome No : 2
  • Gene Starting Position : 219246751
  • Gene Ending Position : 219261616
  • Gene Strand (Orientation) : +
  • Protein Name : solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
  • Protein pI : 7.72
  • Protein Weight : 56200.3
  • Protein Length : 550
  • Protein Note : Also known as LSH; NRAMP; NRAMP1
  • DNA Sequence : Show Sequence
    >gi|224589811:219246751-219261616 Homo sapiens chromosome 2, GRCh37.p5 Primary Assembly
    CACAGAACACGGGGTGCCTGGAAGGGGAACAGATGTGTTGTGGGGCACAGGGCAGGCTGGGAGGGGAACA
    AAGGTCCACTCCATGGGTAACCAGACCCTTCCGCCAGGGCTGGCCACTTCTGCCTTTGGAAAATGTTTCA
    CAACGCCCCATGTTGTGTGTGTGTGTGAATCGGCCGATGTGAACCGAATGTTGATGTAAGAGGCAGGGCA
    CTCGGCTGCGGATGGGTAACAGGGCGTGGGCTGGCACACTTACTTGCACCAGTGCCCAGAGAGGGGGTGC
    AGGCTGAGGAGCTGCCCAGAGCACCGCTCACACTCCCAGAGTACCTGAAGTCGGCATTTCAATGACAGGT
    GAGTAGTGGCCCCTAGGGACAGAGCCTGATTGGGGGGTGGAGTGGAGGAGATCACTAGGCTGGTGGAGAC
    TTGAGGAAGCAAGAAAGCCCTTGGTCCCCTGTGGAAGCCTCTGGTCCCCCGTGGGAGTCCTAGTCTAGAA
    CACAGTTCTGGACACCAGACTCAATCTCACTGGGTGGAGTCCTTCACACCCCTAAGCGGTCTACCTTTCC
    TTTGTCTGAAGTCCGTGGATCAAAGCCCCTGGAGCTTTCTGGGCTGCCGGAGAAGGTGAGGGGTCCAGCC
    AAGGGGCAGACATGTGGCCTCAGCTTGCCCTTCTTAGAGCCTAGGGTCTCTCTGCATTCTCTGTGGCAGG
    AGATCTGCCTCTGGGCATCTGTCCTCACATGAGAGGACAGTAATTCTGAAGCTGCCATGTCCCACACATC
    TGGAATGTTTGTTTGAGAGGTGGAGGTGATGGACCCAAGATGGGGGCCAGGGACCTTAGTTTCTCCACTT
    TTCCGGGAGGAGGAGGGAAAGGATCAGGCGGCTTTAACTCTGGGCCACCAGAAAGAGGCAGGACACTCAC
    CATGCTTCATGGGCCCCCACAGGTGACAAGGGTCCCCAAAGGCTAAGCGGGTCCAGCTATGGTTCCATCT
    CCAGCCCGACCAGCCCGACCAGCCCAGGGCCACAGCAAGCACCTCCCAGAGAGACCTACCTGAGTGAGAA
    GATCCCCATCCCAGACACAAAACCGGTGGGATGCTGGAAACTTCCTGGGGGCTTGCAAGATTGACAGGAT
    CCTGAAGGATCCCATGGGCTGGAGAATGGGGTCTCCTTATGATCATGGGTGGCAAGTCCCTTCCAGGTCT
    GAGCAGCAGTGGTGGACAGTGGCAACTGCCCCTTCCTGCTTCCGTCCCCAGTGCTTAGCTGGGGTGCTTC
    AGTATTTGTCTAAAAGCGATTAAATGACTTTCTCAATGAATCTCAGCTTTCCTATGAAGGAAAAGAGGCC
    CAGGGGGAATTGGGATTGGAGCCTTGAGGACATGTTATAGCATTTAGGAAAGGGCATAGGGAGAAGTGAA
    GGAGGCTGCAGGCTGGCATCTAGCATCTAGGATGCTGTCACCTGCATTTCCAGTATCTTCTCTTTTTTTG
    GAGATGGAGTCTCGCTCTGTAGCCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAACCTCCGC
    TTCCCAAGTTTAAGCGATTCTCCTGCCCCGGCCTCCCGAGTAGCTGGGATTACAGGCATGCACCACCATG
    CCAGGCTAATTTTTGTATTTTTACTAGAGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCT
    TAGCTCAAGTGATCCACCCACCTCGGCCTTCCAAAGTGCTGGGATTACAGACACGAGCCAACGCTCCCAG
    CCTCCAATATCTTCAGCAGATTTCTAAGTCAATATGCCATGCTCTGGAACAGGGTGGGGGGACCATAGCC
    TCAGCGGACCACAGAGAGGAGGCCCCGTGGTCTGAAAAGGAGCTGGCAAGGGAGGAGCTGGGCTAGGGAA
    GCTTGGCCCCCTCACCCCCTTAGACAGCCCCCTGTGAGGTCTCTGAGGACCTGACAAGTCAGGGCTTGAG
    GGGCAGGCAGTTCAGGTGCAAGCCGGCTGCTGAGCTTAACAGATCCAGAAAAAGATCCTCTGTGACTCAT
    CAGAGCATGCTGCTTCCTCCACCCATGCCCTCCCCATCCCTTGGGTGGCAGACCCAGGAATGGGCCATGG
    AGGGCAGGGCTGGGCTGATGAGCCTGTTGGGGCTCCTCTAGGGGATGGCCAAGGCCAGCTGCCACCATCC
    CTATACCCAGGACCCCCTCACTCTACTCCTCCCACCCCCCAACAGGGCACCTTCAGCCTGCGGAAGCTAT
    GGGCCTTCACGGGGCCTGGCTTCCTCATGAGCATTGCTTTCCTGGACCCAGGAAACATCGAGTCAGATCT
    TCAGGCTGGCGCCGTGGCGGGATTCAAAGTAACTAAGTCGGGACCTGAGTGGGGACACTTTCGAGAGGGA
    GGTGACCAGGCTAAGTGTTGAAGGCCCCTGCTGGCCATGTTTCTCCAATGTGGCCTGGGAGCTGGTGTTA
    AGTTCAAATGGGCCCGAAAAGGGTCCCCAGGGCAGCCCTGCCAGAAGGTGGGGCATTTGTGTGGGGGGTA
    GGGGACTGGACTCCTCTCTTTAAAATATATATATGTATATTTATTTATTTATTTATTTAATTTTATTTTT
    TGAGACTCAGTCTCACTCGGTCACCCAGGCTGGAGTGCAGTGGCACAATCTCGGCTCACTGCAACCTCTG
    CCTCCCGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGTGCATGCCACCACGCCTGGCTAATTT
    TTGTATTTTTAGTAGAGATGGGGTTTCACTGTGTGGGCCAGGCTGGTCTTGAACTCCTGACCTCAAGTGA
    TCCACCGCCTCGGCCTCCCAAAGTGCTGGGATAACAGGTGTGAGCCACCTTGCCTGGCGTAATTTATTTA
    TTTATTTAGAGATGGGGGTCTCACTATGTTGCTCAGGCTGCTCTTGAACTCCTGGACTCAAGCAATCCTC
    CCACCTTAGCCTTTTAAAGTGCTGGGATTACAGGGTGAGCTACCAGCGCCCAGCCAGGATGGGACTCCAT
    CTCTCCCACCCCCTCCCCGAGGAGTATGCTTGGTTAGAGGGTACCACGAGCTCAGGGGCTTTCTGAGGCT
    GGCCTCTCTGGCTGAAGGCCTCTCCCTGCCTCCTCACAGCTTCTCTGGGTGCTGCTCTGGGCCACCGTGT
    TGGGCTTGCTCTGCCAGCGACTGGCTGCACGTCTGGGCGTGGTGACAGGCAAGGACTTGGGCGAGGTCTG
    CCATCTCTACTACCCTAAGGTGAGCTTGGGGGGCCTGGACAGGGAGAACCACTGGCCCCAAACCCCAAAC
    AGCCATTTTCAGCTTCCACGATCAAATAAATACATCATCTCACATGGGGCATCCCAAGTCTGTTTGTTTT
    TGTTGTTGTTTGTTTGTTTTTCAGTCGGAGTCTCGCTCCGTCGCCCAGTCAGGAGTGCAATGGTGCGATG
    TCAGCTCACTGCAACCTCTACCTCCCAGGTACAAGTGATTCTCCTGACTCAGCCTCTCAAGTAGCTGGGA
    TTACAGGCGCCCACCACCATCATCGGCTAATTTTTGTATTTTTAGTAGAGCTGGGGTTTCACCATGTTGG
    CCAGGCTGGTCTCAAACTCCTGACCTCAGATGATCCACCCACCTCAGTCTCCCAAAGTGCTGGGATTACA
    GGCATGAGCCACTGCATCCGGCCCCAATTCCATTTGATAGATGGAAAGACTGAGGCTCAAACTGATAGCA
    CATAGCCTAGAGGGACATGAGAGGCATGTCATTTCACAGAAGGGGTAATTAAAATTTGGGAAAGAGACTT
    GCCCAAGACAAAAAATGGGGAGGCATCAGCTCAGGAATGGTCACTGGTTGGGGTGGACCCTGGGGACTTC
    GTTGTTGTGAGTGTCCAGTTTGGAAAACAGAAATTACAGCACTGGACATTTCAAAGGTATTGAATGCAGG
    GATTGAAAGGTTTGGGAGGGCTTGAGGGGCAAGGGAAGAGGGTGTTACAAAAAGATCAGAAAGCTGCTAC
    ACCTCTGGCACAAGCCCCAGAGCCATCCTTTGCTGCTGAGCTCCTGGAAGCATTATTGATGCTGATGAAA
    TGGCCACTAGTGCTGCCAGAGTCCGGGTTGCCTGGTGCCACTGCCAGAAATACAAGAAGCAGGGGGAGAA
    AATGGTTTATCTCAGCCTGGGGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGT
    GGATCACCTGAGGTCGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAAAAATA
    CAAAACTAGCCGGGCATGGTGGCACATGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCG
    CTTGAACACGGGAGGCGGAGATTGTGGTGAGCCGAGATCATGCCATTGCACTCCAGCCTGGGCAACAAGA
    GCAAAACTCTATTTCGAAAAAAAAAAAAAAAGTTTATCTCTGCCTTTCCATCTCCTGTATGCTCTTCCTA
    CATAAGGTAGGAAGCCCATTGGCCAGACTGTCTAGTAAATGTAGTCTGAGACGACAGACAAATGTAGTCT
    GAGACGACAGGCAAATAACCGGCCCACCCTTAATGAAGGATCATCTCCTCCCCATAGGTGCCCCGCACCG
    TCCTCTGGCTGACCATCGAGCTAGCCATTGTGGGCTCCGACATGCAGGAAGTCATCGGCACGGCCATTGC
    ATTCAATCTGCTCTCAGCTGGACGGTACCACCCCAGTGTACCCCAACTCTTCAGGCCAGGCAGAGAACAG
    CTGCTGCTACTTCCCCCCCTAACCAGTCCCTCCCAGAGTCTATTTTATCCTGCTGTCCCCTCTGAAGCAG
    GGCTGCTGCCCTGTTTTCCAGAAATGTAAAGTGACTTGTCTAAAGTCACACAGATGTGAGTCATGCAGGA
    CTTTGGGACTGCAGCCCCAAACTCCCTGCTGCGCCGGGTGCCAGGTCTCTCCTCTAGCTCTGCCCTGCCT
    CGACTGTTCTATGCCACACTCCCACTCCCCTTGCCCTAGCTGTCTGGGGGCGCTTAGGGTCCTGCTCCCA
    GGAGGCCAGATTCCTGTCTCCAGCCCTGAGGCTCCGTAGGAGTTAGAGACCCCTGGACCAGGCTGGGCTG
    ACCCGGGCCACTCTGGTTTCAGAATCCCACTCTGGGGTGGCGTCCTCATCACCATCGTGGACACCTTCTT
    CTTCCTCTTCCTCGATAACTACGGTGGGTGCACACCCCACCTCATAGGGGAGTGGTGGTGGTGAGGGTGC
    TGTACTGGGAGAAGGGCTCTGACATCGAACAGCCTGGGAGCGCACCTGAGCTCCCTCACTCTCCCTGGGT
    GGCCTCTAGCGAGTTACTTGGACGTGCCTCTCTTCACCTGTAACATGGGGAAATAATAGCACAGACTTCA
    GAGGGTGGTTGTGAAGACTGAGTGAGGTAACATATGTTAAGCACTTGGCACTGTGCCTAGAAGCGCTCGT
    AGTATTAGCCATTACGAATTGTTGATGTCACAGATTTTTTTCGTTGGTTTGTTTAGTTTGTTTGTTCTGC
    TCCGTAGGGCTGCGGAAGCTGGAAGCTTTTTTTGGACTCCTTATAACCATTATGGCCTTGACCTTTGGCT
    ATGAGGTAGGAAGCCAGTGCTGCAACCCCACTGTGGACCTCCCAAGATCATTCCTCTCCCTTCCCTCTGG
    CCGCGGGCTGCGGGGGGCTGGGGTGGGATGGAGGCTGAGAAATGGTGACCGCGGCGTGGTTGCGAGGGGC
    GGGGCTTGTCCTGACCAGGCTCCTCCCTGCAGTATGTGGTGGCGCGTCCTGAGCAGGGAGCGCTTCTTCG
    GGGCCTGTTCCTGCCCTCGTGCCCGGGCTGCGGCCACCCCGAGCTGCTGCAGGCGGTGGGCATTGTTGGC
    GCCATCATCATGCCCCACAACATCTACCTGCACTCGGCCCTGGTCAAGGTGAGCAGAGGGGAGGGGAAAG
    GAGACCCCCTCACTCAGTCGGAGCCATGCTGGCTCCGCCTCCAAGCCTGGAGCCCCTCCCCTCTGGCTCC
    TTCCCTCAGGATTTGCGGAGCCTGTAATAATTGAGGGACAGGCGGGGCGCCATGGCTCACGCCTGTAATC
    CCAGCACTTTGGGAGGCGGAGGCGGGCGGATCACGAGGTCAAGAGATCGACACCATCCTGGTCAACATGG
    CGAAACCCCGTCTCTACTAAAAATACAAAAACTAGCTGGGCGTGGTGGCGCGCGCCTGTAGTCCCAGCGG
    CTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGGTTGCGGTGAGCCGAGATCTTGCC
    ACTGCACCACTCCAGCCTGGCGACAGAGCGAGACTCTGTCTCAAAAAAAAAAAAAAAAGAAAAGAAAAAG
    TGTCTACTGTGCGCTGGGAGGAACAGACCGTCAAGGCCTCTGCCCTTCTAGAGTTTCCGTGCTAGTGGGG
    TAGACGGATGACAAGCAAGTAAACCAACAAAGGAAAGAATTATAGGCCGGGCGCAGTGGCTCACGCCTGT
    AATCCCAGCACTTCAGGAGGCCGAGGCGGGCGGGCGGATCACGAGGTCAGGAGTTTGAGACCTCAACAGC
    CTGGCCAACATGGTGAAATCCCGTCTGTACTAAAAATACAGAAAATTAGCCGGAAGTGGTGGCGCGTGCC
    TGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAAGCCAGTAGGCGGAAGTTGCAGTGA
    GGGGAGATGGTGACATTGCACTCCAGCCTGAGTGACAAAGCAAGACTCCGTCTCAAAAAAAGAAAAAGAA
    CAAGAACTATAGAGCTGGGCGAGGTGGCTCACGCCTGTAATCCCGGCACTTCAGGAGGCAGAGGCAGGTG
    GATCGCTTGAGCCCAGGAGTTCGAGACCACCCTGGGCAACATGATAAAACCCTGTCTCTACCAACAAAAA
    ATAAAAAATAAAATAAATTAGTTGGGTGTTGTGCACACCTGTAGTCCTAGTTACTCAGGAGGTTGAGGCT
    TGAGCCCTGGAGGTCGAGGCTGCAGTGAGCTGAGATCCCACCACTGCACTCTCAGTGACACAGTGAGACC
    CTGTCAAAATAATAATAATAATAATAATAATAATTGCGAAAGGGACTCTGAAGTAAACAAAACAGAGAAA
    TATGATAGAGAATGACTGAAAGTTGGGGAAGTAGATCAGGGAAAAGATATTACAAGCGGAGGGAAAGCAA
    GTGCAAAGGCCACTGGCAGAAGTGAACAAGGCTTGGGTCCCTAACAGCTTAACAGTGGCTCACACCTGTA
    ATCTCAGCATTTTAAGAGGCCAAGACAGGAGAATGCTTGAGCCCAGGAGTTCAAGGCTAGCCTAGCAAGA
    CCCTGTCTCTGCAGAAAATTTAAAAATTAGCGGGTTGTAGTGGTGTGTGTCTGTGGTCCCAGCTACTTAG
    GAGGCTGGGGCAGGAGGATCACTTGGGCCCATGAGTTGGAGGCTGCAGTGAGCCATGCATTGCACCACGG
    CACTCCAGTCTGGGTGACAGAACAAAACCTGTCTCTAAAAAATAAAATAAAGTAAGCTGGACACGTCTGA
    GGATGGAACAAGGTGAGTGAAGGAGCGTGTCAGGACCTGAGGTAGCCAGGGACCTCAAAGGCCAGCCTTG
    CTTCACCCACACAGTGGCTTACAGTGGTAAGGCCTCTGTGGCAAGAACAGAGATGTAGGAAACCATCGGC
    TGACCTGAACCTGCCCCAGACTGCCACGCAGGGGACTTAAGAAGGTACTGGGCTTTGGGGAGAACATAGA
    AGTGTGAGGGTGGGGGACACTGTGGTGGCTCTGAGGGACTTTGGCACTTCCCTCTCCCTTTGATCTTCGT
    AGTCTCGAGAGATAGACCGGGCCCGCCGAGCAGACATCAGAGAAGCCAACATGTACTTCCTGATTGAGGC
    CACCATCGCCCTGTCCGTCTCCTTTATCATCAACCTCTTTGTCATGGCTGTCTTTGGGCAGGCCTTCTAC
    CAGAAAACCAACCAGGCTGCGGTGAGACACACTTTCCCCCGCACCTGAGGCCACACACGTACTCATGTCC
    TGTAAGCCTTGCCGAGGACCCTAGGCAATGCAGCTGAGCCCTTCTGAGTCTCTGCCCTGATGATCTTCCC
    TGTTGGCAGATATCATTCATTCAGCAAATAATCATTGAGCATTTGTTATATACCAAGCACATCCTAGACC
    CTGGGGATACAGCAGTCAATGCTACAAAGACCCAGCTCTCTGCAGCTTAAATAGGGAGGGAGGTGAGCAG
    ACAGGAATGAATACCCAAGGGAAGTACAGAGTGGGAAGAAATAAAGGGGGTGAAGAAGCATAGTTGGGGG
    TATGGGGGTGGGGTCTGCATTAGCCCAGGTGGTCAGGAAAGGCTTCTTGGAAGAGATGACATTTGTGCTA
    ATACTGGAAAAGGAACTAGCCAGCTCCTCCCTGACTCTCTGGGATCTGGGACCTGTGTCTGTCTCCCCTT
    CTGAGTGGATCTCAGTCATGAGGCTCTAACAACTGCAGCCAGGGTTGGGGGCTTCCTGAATGCTGAGCCC
    TGTGGGAGAAGGGGTGGCCAGGCCTCCAAGGACCTGGGCACTGCCACTGCCTTGCTCTGTGACCTTGGGA
    GGTCACACTCCTCCCTGGGTCTCACTCTGTTCCTTGGCACAAGGAGGGAGATCAACATCAGGACTGCAAA
    CCCTTGTCTTCAAGACCAGGCAGGCGCAGAGTTGTGGGAAGTGGACAGTGTGAAGAGGGCATGGCAGCCA
    GCTGTCTCTGTGGTGATGCCAGCTCTTACGAACCTAAAAGAAGCTGGAAACTGGGTTCTTATGCCAGGCC
    TTCTCAATTTTAAATGTTGGCAACAAATCCACATTAAAGAAAAAAAAAACCCGGCCGGGTGCGGTGGCTC
    ACGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGATGGCTCGCTTGAGCCCGGGAGTTCAAGACCAA
    CCTGGGCAACATGACAAGACCCCCATCGCTACAAAACGTAAAAGTAAAACAAAACAAAACCCAACTTATT
    TTGTTAGCCAAACAAAATATGTCTGGCCAGATTCAGCCTTGGGCCACCAGTTTTGGAACCCTGGTCAGTG
    CTAGGCAGTCCAGTTTCCCAAGGCTGAGCGTGCTCCTCACATCTTCCTTCTACTGCCCTGGTACCCACAG
    TTCAACATCTGTGCCAACAGCAGCCTCCACGACTACGCCAAGATCTTCCCCATGAACAACGCCACCGTGG
    CCGTGGACATTTACCAGGGGGTGAGCGCGGGTGGGTGGGGAGGGCGTGACCCAGAGAGGCTCCCCGCCTC
    GGGCAGGGCCACCGGTCCTACCACACTCGTCCCTGCAGGGCGTGATCCTGGGCTGCCTGTTCGGCCCCGC
    GGCCCTCTACATCTGGGCCATAGGTCTCCTGGCGGCTGGGCAGAGCTCCACCATGACGGGCACCTACGCG
    GGACAGTTCGTGATGGAGGTAGGGCAGGGGGCGGGCCCAGGAGGGCAAGGGGTCCAAGGACAGCAGGCAC
    ACTACTGGGGGCTAGAACTCCGAGCCTTGTGGGTCCTCTTTTCTTTTCTTTCTTTTCTTCCTTTTTTTTT
    GTTTTTGTTTTTGTTGTTGTTGTTGAGACGGAGTCTCGCTCTTGTCGCCCAGGCTGGAGTGCAGTGGCGC
    GATCTCGGCTCACTGCAACCTCCCCCTCCCAGGTTCAAGCTTCTCCTGCTTCAGCCTCCTCAGTAGCTGG
    GATTTCAGGCACCTGCCACCAGGCCTGGCTAATTTTTGTACTTTTAGTAGAGACAGGGTTTCGCCATGTT
    GGCCAGGCTAGTCTTGAACTCTTGACCTCAAGTGATCCGCCTGCCTTGGCCTCCCAAAGTGCTGGAATTA
    CAGGTGTGAACCACCGCACCCGGCCAATTTTTGTATTTTTAGTAGAGACGCAGTTTTGCTGTGTTGGCCA
    GGCTGGTCTCAAACTCTTGACCTCGTGATCCGCCCGCCTTGGACTCCTGAAGTGCTGGGATTATAGGCGT
    GAGCCACTGCGTCCGGCCTTTTCTTTTCTTTCTTTCTTTCCTTTTTTTTGAGACGGCGTTTCGCTCTTAT
    TGCCCAGGCTGGAGTGCAATGGCGCGATCTCCGGCTCACCGCAACCTCCACCTCCTGGGTTCAAGCAATT
    CTCCTGCCTCAGCCTCCTGACCAGCTGGGATTACAGGCCACCACACCCAGCTAATTTTGTATTTTCAGTA
    AAGACGGGGTTTCTCCATGTTGGTCAGGGTGGTCTCGAACTCCCAGCCTCAGGTGATCTGCCCTCCTCAG
    CCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCTCTTTCTTTCTTTTTTGAGACAGGA
    TTTCACTCTGTTGCTCAGGCTGGAGTGCAATGGCGCCATCACAGCTCGCTGCAGCCTCAACTTCAGCCTC
    CCGAGTCATTGGGACTACAGGCTCATGCCACCACACCAGGCTAATTTTTAAAATTATTTGTAGAGATGTG
    GTCTTCCTATGTTTCCCAGGCTGGTCTCGAACTCCTGGGCTCAAGTGATCTTCCCGCCTTGGCTTCCCAA
    AGTGCTGGGATTACAGACGTGAGCCACCCCACTCAGCTTTGTTTCCTTCATGTAGCCCAGAGGGCGACAC
    TATAAGCCCCATTTTACAGATGAGAAAACAGATCCTGACAGATGAGGTGACTTGCTGGAAGCTGCAGACC
    CAGGACTAACAGAAGCCTTTTCCCCAAAACTGTTTAGTCTTCAGCAACCAGCTATGGGATGGGAGCTCCC
    CATTTCTCCCCACCCATCCCCTCTTGCCACCTAGGGACAGAGCTGTCCCAGTTCAACAGTGGAAAAACAG
    AGCATGCCCCCAGGGATAAATCGGTTGAGGGACTACAGAGGATCTCTCCTCTGGAATCCCCAGTCCTGTC
    TACTCCTCACCAAGGAGTTCACCCCCACCCCAGGGCTTCCTGAGGCTGCGGTGGTCACGCTTCGCCCGTG
    TCCTCCTCACCCGCTCCTGCGCCATCCTGCCCACCGTGCTCGTGGCTGTCTTCCGGGACCTGAGGGACTT
    GTCGGGCCTCAATGATCTGCTCAACGTGCTGCAGAGCCTGCTGGTGAGATGCGCCAACCCCACCAGCCCT
    GCCCACTGCTGAGCGAAACAGGACCTCCAGCAACCCCCACGCTGAGCCTTGCATGGGCCTGCCAGGCCCT
    GTCCCAGGCACATCTTGCCTACAAGGCTTCCATTGTCCCCACAGCCACCCTGGGGGGCAGGAGGGTAGGA
    ATCACCATTATCCTTCCCCCCTCCCACCCCCCAGATGGTCTCCCTCTGTCACCTAGGCTGGAGTGCAGTG
    GTTCAATCATGGCTCACTGCAGCCTGCCCTCCCTGTCTCTAGCAATCTTCCCACCTCAGGCTCCCGAGTA
    GCAGGGACTATAGGTGCATGCCATCACACCCAGCTAATTTTTTTTTTTTTTTTTTTGAGACGGAGTTTCA
    CTCTTGTTACCCAGGCTAGAGTGCAGTGGTGAGATCTTGGCTCACTGCAACCTCTGCCTCCCAGGTTCAA
    GCGATTCTTCTGCCTCAGCCTCCTAAGTAGCTGAGATTACAAGCGCCCACCATCACACCCAGCTAATTTT
    TTGTATTTTTAGTAGAGACAGGGTTTCATCATGTTGGCCAGGCTAGTCTCAAACTCCTGACCTCAGGTGA
    TCCACCCGCCTTGGCCTCCCAAAGTGCAAGGATTACAGGCATGAGCCACTGAGGCAGGTCTAATTTTTTT
    TTTTTTTTAAGTAGAGACAAAAGCTCATTATGTTTGTTGCCCAGGCTCATTATTCCATTTTACAGATGGG
    AAAACTGATGCACTCAGTTAAGTTACTAGTGGCAGGGCTTGGATTCAAACCGAAGCAACCTGGTGCCAGA
    GCCCCCAAACACTTTCTCCTGCTTTGGAGCCCACAACCTGATAAGGCAGGAGACGGGGAAGTAGGCTCAG
    TGTGGTTAGGGCAGTTGAGCTCACACCCACACAGAGGGTGTCAAGCCACGCCCATCTTGCCCCCACCCTT
    GCCATATTCTGAGGCAGAATTCCTCAGCCTAGGCTCCTGCTGCTCTCCCCAGCTCCCGTTCGCCGTGCTG
    CCCATCCTCACGTTCACCAGCATGCCCACCCTCATGCAGGAGTTTGCCAATGGCCTGTGAGTACCCCCTT
    TCCCAAGTGCTGGATTGCATCACCACATTTCATCCTCACAGCCACCCAGAGGTACGAGCTACAATTCTCC
    CCATTATCCCAATGAAGCCACAGAGGCTGAGAGAGGTTAAGGGACTTACCCAGGTTCACACAGCTAGCAA
    GTTGAGGAGCCAAGACTGGAATCCAGGGAAGAGGGCTGTTTCTAGAGCCTGAGCGCCTCACTCTCGCCTC
    AGACTCAGCTCTTGCCAAATCCCGCCAGTGTGTAGCCTGGAGGGCCCAGGAGGCAGGAGGTGGGGAGGGG
    GTCTGTCTGCTCCAGGTCCCACAGACCAGAGAAGCGGCCTCAGTGTATCCCCACCCCCAGTGTGGGCGCT
    GGGAGATGAAGAGGAGTTGATGCAGGTGGGCCAGCAGCAACCAGCCAGTCCTGAGCCTCTCTCGTGTCCC
    CCAGGCTGAACAAGGTCGTCACCTCTTCCATCATGGTGCTAGTCTGCGCCATCAACCTCTACTTCGTGGT
    CAGCTATCTGCCCAGCCTGCCCCACCCTGCCTACTTCGGCCTTGCAGCCTTGCTGGCCGCAGCCTACCTG
    GGCCTCAGCACCTACCTGGTACAGTAGGGCCAGGGGATGCCTTGGGAATGGATGAGGGAAGGACAAGAGG
    CAACCAATGGGGAGGGTTTGGGGGGACACAATGGGGCTTCCCCAGAGGTCTTGGCATCTCCCCAATTCAT
    GGTTGCCCCTCCCCCAGGTCTGGACCTGTTGCCTTGCCCACGGAGCCACCTTTCTGGCCCACAGCTCCCA
    CCACCACTTCCTGTATGGGCTCCTTGAAGAGGACCAGAAAGGGGAGACCTCTGGCTAGGCCCACACCAGG
    GCCTGGCTGGGAGTGGCATGTATGACGTGACTGGCCTGCTGGATGTGGAGGGGGCGCGTGCAGGCAGCAG
    GATAGAGTGGGACAGTTCCTGAGACCAGCCAACCTGGGGGCTTTAGGGACCTGCTGTTTCCTAGCGCAGC
    CATGTGATTACCCTCTGGGTCTCAGTGTCCTCATCTGTAAAATGGAGACACCACCACCCTTGCCATGGAG
    GTTAAGCACTTTAACACAGTGTCTGGCACTTGGGACAAAAACAAACAAACGAAAAACATTTCAAAAGGTA
    TTTATTGAGCACCTGCAGGCGTGACCTGACAGCCCAAGGGTGGGTGGGGTGAGGGCTTGAGGACTTGGGC
    GGGACACAGGCTCCAAACTGGAGCTTGAAATAGTGTCTGATGAATGTTAAATTATCTATCTATCTATTTA
    TTTATTTATTTGAGACAGGGAAAGGGTCTCCCTCTGTTGCCAAGGCTGGAGTGCAGTGGCGCAATCTTAA
    CTCATTGCAACCTCCACCTTCTGGGTTCAAGCGATTCTCTTTATTCAGCCCCGGGAGTGGCGCGCGCCAC
    CACGCCCAGCTAATTTGTGTATTTTCAGCAGAGACGGGGTTTGCCATGCTGGCCAGGCTGGTCTCGAACT
    GCTGGATTCAAGTGATCCGCCCATCTCCGTCTCCCAAAGTGCTGGGAATTACAGGCGTGAGCCACCAAAC
    CCGGCCTGATTAAAGTTAAATAAATACTAGTTCCCTTCTCGTCCAAAGGAGCAGGGAATGGGAACCGGGA
    AGGCACGAAGTCTCTAAAGCATCCAGAAGACCCCTACACCAGGGTCTGGTCCGCTCCTATTCGCCGCAGC
    CTTTCTGTTCCGCCTGCAACCCATTTTCCAGACAGTAAAACGGCGGCGCACTTCTTTCTCCGTCAGGCAC
    CAGGTCATAAGGAACCCAAGAGTCTGTGCCTCTGAGGCCCAAATTATTTGCTGTTTCCTCAGGGGAGCCG
    GCGGCCGCGACTCCCACGCCGCGCCGTTACCGCTCCCTCTCTGCTGACTGCTCCCCCTAGGGGCAGAGAC
    GGTCCCGACGCCCGCCATCCCGCCCCGGCCTCACCCCTCCCCGCCAGGCGGAACGACGCGGGGAGGCGGG
    CGCTCGGGGCTCGCGCCAGGGGCCCCAGAATCCTTCGGGGAGAGTGGGTGGGAGGAAGCTGTGTGGGCGG
    GGAGCCCCCTCTGCCTTAGGGAGCGGCTGGGCACCCATTCGCCCCATTCAGGGGCTGCACTTTATAGACG
    TTCCCTAGGCTGTTTCTAGGCTCCCCCAAGTCCCTCCTCCAGCCTCGTCGGGTCCCTCAGACCCCAGCCC
    AGGACCTGCGGAGGGCCGCAGCGAGGAGAGGCCAACAGGCCTTTCCCTAGAGTTGAACCTGGGCCGGGTG
    TTGCACCTGGAAGAACCCCCGATTTCCTGGGGACCCAGCAGGGCAGGCGGCCTGGCTCCGCGCTCAGGTC
    CGGACGCTTGTTTATGAGAAGAATTTCCTCTTTCTTAAAAGGGCAACGATGCGAGTGGGTCCCTCAAGGA
    GAGAAGAGATGGGACCGGTCTGGTGCGACCTGGGCAAGCGCTGCAGAGGGTACCTGGGCAAGAGGGCCGC
    CCGCCTCCTCTGGGTTTGGCACTGGAGAAGATGGGTCCATGCCAGCTGAAGGAGGAGATGGATGGGGGAC
    GTTTAGCGAAGAAAGGCATCTCCCAGATCCTTTAGCCTCCTGGAAGTGCCCCCGTTGTACCCCCTACACA
    CCCCTCTTGGCATTGAGTGCCAGTCCTCTGCCAGGCTCTGTGTTACAAGTTGGGGAGGGCGGCAAAGTCC
    CGAATTAAAGATGTCAGTTCTCAAGG
  • Protein Sequence : Show Sequence
    >gi|109255241|ref|NP_000569.3| natural resistance-associated macrophage protein 1 [Homo sapiens]
    MTGDKGPQRLSGSSYGSISSPTSPTSPGPQQAPPRETYLSEKIPIPDTKPGTFSLRKLWAFTGPGFLMSI
    AFLDPGNIESDLQAGAVAGFKLLWVLLWATVLGLLCQRLAARLGVVTGKDLGEVCHLYYPKVPRTVLWLT
    IELAIVGSDMQEVIGTAIAFNLLSAGRIPLWGGVLITIVDTFFFLFLDNYGLRKLEAFFGLLITIMALTF
    GYEYVVARPEQGALLRGLFLPSCPGCGHPELLQAVGIVGAIIMPHNIYLHSALVKSREIDRARRADIREA
    NMYFLIEATIALSVSFIINLFVMAVFGQAFYQKTNQAAFNICANSSLHDYAKIFPMNNATVAVDIYQGGV
    ILGCLFGPAALYIWAIGLLAAGQSSTMTGTYAGQFVMEGFLRLRWSRFARVLLTRSCAILPTVLVAVFRD
    LRDLSGLNDLLNVLQSLLLPFAVLPILTFTSMPTLMQEFANGLLNKVVTSSIMVLVCAINLYFVVSYLPS
    LPHPAYFGLAALLAAAYLGLSTYLVWTCCLAHGATFLAHSSHHHFLYGLLEEDQKGETSG
  • Molecule Role : Vaximmutor
  • Molecule Role Annotation : On multivariate analysis, patients with the NRAMP1 (i.e., SLC11A1) D543N G:G genotype and allele 3 (GT)n polymorphism had decreased recurrence time (p=0.014 and p=0.03) after human BCG therapy.
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
37. STAG3
  • Gene Name : STAG3
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 10734
  • NCBI Protein GI : 57863310
  • Genbank Accession : AC005071
  • Protein Accession : NP_036579
  • Taxonomy ID : 9606
  • Chromosome No : 7
  • Gene Starting Position : 94410282
  • Gene Ending Position : 94446766
  • Gene Strand (Orientation) : +
  • Protein Name : stromal antigen 3
  • Protein pI : 5.05
  • Protein Weight : 132581.25
  • Protein Length : 1225
  • DNA Sequence : Show Sequence
    >gi|157734172:94410282-94446766 Homo sapiens chromosome 7, alternate assembly HuRef, whole genome shotgun sequence
    CCGCGCTTTTCTGGAATCTTTCGCCCCCGGAAGGGCAGCGGCGGGCGCCTGTGTGGAAGGTGGGGTGGCC
    AGAGACACCCGAGGGACCTGAGGTGGGGACCTTCCCTGAACCCTGCTCGGCCAGAAGTCACTCTTCCAGG
    CTCTGACAGTGAGGTTTCTCCTTGACCCAAGAATAGGCCCTCTCGCGACCCTCCCGGCCCTGACACTAGG
    AGCCTTCCCGGGCCGTGCCCCCAATGGCCTCCGCCACAGAGCTGGAGAATCCTCTCCCCAGGCCTCCATA
    GACTGAGAGTCCATTCTCTTAAGCGCTTGGAAGGCTCTTTATCCTGGAGCCGCTTACCCCAGGGGCAGTC
    GTAGATATTTTTACCTTCTGGAAAAATAGGAAACGCGCACCTGCCTTTCCTCCTTTTGCGTTTCCAGCCC
    TATTCTCATGGCGCCTCCACCCCCAAATCCTTAAGAGCCTCCTGTTCCATTCCAGGTGGAATCAGGTGCT
    AGTCCTCTGGAAACGAAAGCCTGGGGTCTTCTTGTATTTTTTTTCTTATTTCTTTCTTTATTTTTTTTAT
    TTCATTTTAAAAAACCTGTAAGTTCCCTTGCAGAGGGCGGTTTATACTTCCGAAGCCTCCAGAGAGGGGT
    GCGTCTGAAGCTGCCCTGCTGTCTGCCTTGCTTGGCTTTTTTTTTTTTTTCCATTTAATTTTTTTTTTTT
    AGAGACAGGGCGTTGCTATGTGGCCCAGGCTGATCTTGAATTCCTGGACCCAAGCGATGCTCCCGCTTCG
    CCCTCCCAAAGTACTGGGATTATAGGCGGGAGCACTGTGCACCACGCCTCGGCTTTTGGAGAGCAATTCC
    AAACCCACTGCCCAGTTTCTGGTGTCTGTAATCCCTTGGTGCTTTAGGTCCACTCTTTGCCTTATTTTAT
    TTATTTGTCATTACTTTTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTTTCCCAGGCTGGAGTGCAGTGG
    TGCAGTCATAGCTCGCTGCAGCCTCGACCTCCTGGGCTCAGGCGATCGTCCCGCTTCATTCTGCCGAGTA
    GCTGGGACTACAGGTGCACCACCACACCTGGCTAGTTTTTTTGGTTTTGGTTTGGTTTTTTCGTAGAGAC
    AGGATCTTGCTATATTGCCCAGACTGATTTTGAACTCGTAGGGTTAGGCAGTCCTCCTGCTTCAGCTGCT
    CAAAGTGCTGGGATTCCAGGTTTGAGCCACCCTGGGCACCTTGCCTTTTTTTTTTTTTTTTTAATTATGA
    GTTAAATTCCCTTGCAGCAGGCAGTTTACACTTCTGAAGCCTCTAGAGAGGGCTGTGTCTGAAGCTGCCC
    TGGTGTGTACCTTGCTTGGGTTTTGTTTTTTTTTTTTTGGGTGTGTCTCATGTTGCCAAGGCTGGTCTCA
    AACTGCTGGGCTCAAGCAGTCCTCCTGTCTCAGCCTCCTGAGTAGCTGGGACCACAGGTGTGCGCAACAA
    CACCTAGCTAGTTTTTGCTTATTTTTTGTAGAGATGGGGTCTTGCTATGTTGCCCAGATTGGTCTTGAAC
    TACTGGGCTCAAGCAGTCCTCCCGCCTCAGCCGCCTAAAGTTCTGGGATTACAAGTATGATCTACCACAC
    CCAGTGTGCAATTTTTTTTTTTAAAGAAAATACCTATGAGTTAAATTTCCTTGCAGTAGGCAGTTTGCAC
    TTCCACAGCCTCTAGAGAGAGATGTGTCTGAAGCTACCCTGGTGTGTGACTTCCTCAGCTTTTGGAGAGC
    AACTCCAAACCTGCTGGCCAGTTTCTGGGGTGTTGTTGTGCTTAATCCCTTGATGCCTTAGGTCAGCTCT
    TTGTCTTATTTATTTATTTATTTTGAGATGGAGTTTCACTCTTGTTGCCCAGGCTGGAGTGCAGTGGCAC
    AATCTCAGCTCACCACAACCTCCGCCTTCTAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCAAGTAGCT
    GGAATTACAGGCATATGCCACCACACCCGGATAATTTTGTATTTTTAGTAGAAATGGGGTTTCTCCATGT
    TGGTCAGGCTGGTCTCGAACCCCTGACCTCAGGTGATCTGCCTGCCTCGACCTCCCAAAGTGCTGGGATT
    ACAGGCATGAGTCACTGTGCCCAGCCATTTGTCTTATTTATCATTACTATTTTTTTGAGGGGGGACAGGG
    TCTTCTTCTGTCACCCAAGCTGGATGGAGTACAGTGGTGCGATCGTAGCTCACTGCAGCCTTGAACTCCT
    GGACTCAAGCAATCCTCCCACCTCAGCCTCCTGAATAGCTGAATACAGGCATGCTCTACCACACCTGACT
    AATGATTATTATTATTTTTTTTTTAGAAATGGGGTCTTGCTGTATTGCCCAGGCTGGTCTTGAACTCCTG
    GGCTCAAGCCATCCTCCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAGGCATGAGCCACGGTGCCCAGC
    CTTTGTCTTAGTTTTTGGTCCCCCTCACCTGACCCCACAGTTCTAGAACCATTCCCTTGGAAAGAAAGGG
    ACTGGTCAGGGCTGGGGATGGAGAAGTGCTGTGGTAGGAGCCCTTTCTTCTCTTTCTTCCCCAGCTGGAT
    CGCCATACCTACCCTGTGGTCCTCATCTTCCTGGCCTCATAGCTCCTCCTCTCCAAGCATGTCTTCCCCG
    TTGCAAAGAGCTGTGGGAGATACCAAGAGGGCCTTGTCTGCATCTTCTAGTTCCTCTGCCAGTCTACCCT
    TTGATGACAGGGACTCAAACCATACCTCAGAGGGGTAAGTAGATGTTGCATTGTGTGGCCACTTCCTGTG
    TCCCTGGCAGCCTTCCCCCTCGTTTTCCCACATTGATGATAATATGCGTGGGACTGTGTGAAAACACCAG
    AAGTGCTATACTCGTTCAGCACAGTGACACCCTCCCTCCAGCTCCAGGTGTTTTTTTTGGCGGTCCTAGA
    TGTTGTAGACATCAAAATAGTAGTACATCCTGTTTTTTTTTTTTTTTTTGAGACGGAGTCTCGCCCTGTC
    GCCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTTACTGCAACCTCCGCCTCCTGGGTTCAAGCACATCC
    TGTTTTTAATCCATCATCCATCTTGTCTAAACCTCTCTTAAACACATTTCTATGTTCAGCATGTACCAAC
    CCTGGTGGGGGCTGAGAGCTGGCAGATGGGGGAGTAAAGAGTTCTTTATTTGTTGCCTGGTAAATTGTAT
    ATACTTTTTGGCTAGTGTCCCCTATTTCTTCTACTTGGGGATTTTGTAAATAGGTCTGTGTATCCTTATT
    TATTACCATATATGAGTTTATTCTGTGTAGGTTATAATAATATATCTTGGTTTTTTACCTTTCCAGAAGG
    AAAAAAGCGAATTTCATACAGATCTGCTTTATCCCCTGGCTGTTTTAATTGGCTTTCTCTGAATCATTTC
    TGTTTCTGTCAGTGCTGCTGTGGGGGACCAGATTATATCAAAGTTATAAACTTGAATTGATAAGACATGT
    GGTTTATGTTTCAAGGGGGTGAAATTTAACTCTAGATACATATTACTCTCCCATTTATGGATGAATGATA
    GATGACTTCCTGAGCCATGATTATCTTTCTCTACAAAGAGCAGTGATCTCGCTACCTGCTAAGTGAGTAT
    CATTTGATTCTCTTCTTCGGAGAGAATACTAGGAAGAGTTGTCCTTCTCCTGCCTCATTTCCTCATTCTT
    CGTCTCCATTGCTTTCACGTGAAAGTTTTCCTTTAAGGCTTTGTCCCGGCCGGGTGTGGTGGCTCATGCC
    TATAATCCCAACACTTTGGGAGGCCGAGGCGGGTGGATCACCTGAAGTCAGGAGTTTGAGACCACCTTGG
    CCAACACGGTGAAACACCTGTAATCCCAGCTACTCGGGAGGCTGAGGTAGGAGAATCGCTTGAACCTGGG
    AGGCGGAGGTTGCAGTGAGCTGAGATCATGCGATTGCACTCCAGCCTGGGTGACAAGGGTGAAACTCCGT
    CTCAAAAAAAAAAAAAAAAAAGACTTTGTCCCAACTTCCTACTAACAATAAGCTCATTTTTTCTTAAAAT
    CTGGATATGTTTTTCTCATGGAGATGGGGTGCGGTGAGAGACAGAGAAACCATAATTAATTAGCCTTTTA
    TATGGAGGGAATAGGGTGGTTATATTTAAAGAGAACCATACTTTCTCACAGGAATGGCGACTCTTTGTTA
    GCTGATGAAGACACTGACTTTGAAGACAGCTTGAATCGCAATGTGAAGAAGAGAGCAGCAAAACGACCAC
    CGAAAACAACACCGGTGAGTCAGCCAGTTTTCTTTTGTTTTTGAATCTTGTGGGGGAAGCAGCCCAGCTA
    CTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGGGGCAGAGCTTGCAGTGAGCCGAGATTGCGCC
    CCTGCACTCTAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAGAAAAGAAAACCTAAAA
    GAGGTTCAGGTGATACGGTTCATAAAAGGACAAGATTTACAAAGTATTAAGGGATGGAAATAGGAAGAAG
    AGAAATTTTGAAGAGAAAAGAAGAGAAAAGGACGTTAGGATGTAGAGATACAGGAAGGGGACTCAAGGAC
    TTTTTTTTTAAGGGAAAAAAATAAACTTCATAACAATATAAAAATCAATTATTTCAGGACTGAGTCTAAC
    TCAGACTCTGCTGTGGAATTGGCAGCTTAAGCCTACTTGTGTGATAATGATGAAACCAAGCGTTAATGTC
    ACTGTTACCTTTTTTGTTTTTTTTTCATATTTCTGATCTTTTTATACATATTAGGTGGCAAAACATCCAA
    AGAAAGGGTCCCGAGTGGTACATCGTCATAGCCGGAAACAGTCAGAGCCACCAGCCAATGATCTTTTCAA
    TGCTGTGAAAGCCGCCAAAAGTGACATGCAGGTAAAGCAGTGTCTCACCTCTGTTGATACCATCTCACTT
    TTTGTAAGGTGGTAAGGACAAGTGTCTACAAACTTGATTTGACGTGAACATTTTAGTGAGTGTAACATGT
    CAAGAAAGATAAGGTTATGGGGTGAATCCTTGGAGTGATAGAAGGGTAACCTTGAATGAGAGAGCATGTT
    CTTTTTTTTTTTTTAGACAGAGTCTCTTTCTGTCGCCCAGGCTGGAGTGCAGTGGCACAGTCTTGGCTCA
    CTGCAGCCTCTGCCTCCTGGGTTCAAGCGATTCTTGTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGC
    GTGTGCCACCACGCCCAGCTACTTTTTGTATTTTTAGTAGAGATGGGGTTTCGCCATGTTGGCCAGGCTG
    GTCTCCAACTTCTGACCTCAGGTGATCCGCCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAG
    TCACTGCACCTGGCTTTGAGAGAGCATGTTCTTTTCTTAAAGGTATCACAGGAGTGATTATGCCTTTATT
    GATTGATGGAGCAGATGAGGAGAGGCATTGCATGACACAGGAAGGAAGCTTGGATGTGGGTGGCACCTTG
    TTCACCCATGTTATTTTTTAGCTCCATCTTGACAATTATACTTAATTATTTTTTCCTTGTTTCTTTTTTT
    ATAATTTTATTGAAGTTCTAATACCCGAAAGTGTACAAAGCTTATATGTAGTTTAAAAAATAAAAATGAA
    ATGATTACTCAGATCTCTAGCCCCAGCTCAAGAAATGAAACATTAATAGTGCTGTAGAGATCCTTTGTGT
    ACCTTCTTTCTACTCCATTTCACAGTCTCCTTATTACCCACAGGTAAACACTAGAGTGAATTTTGTGTTA
    ATCTTTCTCATCTCTCATTATAGTGTTACCACTATGAATATGTTCTTTAATTATGTAAATGAATCCATTA
    TTCAGGCTTCTATGATTTAATTATTTGAGTTAACATTGTTTGAGATTTACCCACATTAATGTGTAATTTT
    ATAGTTTCTGGGAAAATATTTAAAATTTTCAAACATATAGAATTTTGCTAGTTATATTTTCGTTATTTGA
    TTTCTAACTTCATTGTGTTGTAGTCAGGGAATGTGGTGTTTGAAATTTATGTAGGGGCCGGGCACAGTGG
    CTCACACCTGTGATCCCAGCACTTGGGGAGGCTGAGGCGGGCAGATCACTCGAGGCCAGGAGTTTGAGAC
    CAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGCATGTGGTGGCGTTT
    GTCTGTAATCCCAGCCACTTTGGAGGCTGAGGCATGAGAATTACTTGAACTCGGGAGGCGGAGGTTGCAG
    TGAGCTGAGATTGTGCCACGGCACTCCAGCCTGGATGACAGAGTGAGACTCTGTCTCAAAAAAAAAAAAA
    GAAAGAAATTTATGGAGACTTAGCCTTATGGCCTAGTATTGAGTTGGTACATTCGTTTTATCTTTTCTGT
    TTCTTTTTCCACCTTTGCTTGCCTTCCTTTGGATTTCTTATCTTCTGCAAGGACCTGAGCACATTTTAAT
    TCCAAATGTCTTTTCCACCTTATATGCAGTTGTACAGCGTGTTAGGTTTTTTTTTTTTTTTTTTTTTTTT
    TTGAGACGGAGTCTTGCTCTGTCGCCAGGCTGGAGTGCAGTGACGTGATCTTGGCTCACTGCAACCTCCA
    CCTCCCGGGTTCACATGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGATGCATGCCACCAC
    GCCCAACTAATTTTTGTATTTTTAGCAGAGATGGGGTTTCACCGTGTTGGCCGGGATGGTCTCGATCTTT
    TGACCTCGTGATCCGCCGGCCTCGGCCTTCCAAAGTGCTGGGATTACAGGTGTGAGCCACCATGCCTGGC
    CTTTTATTTCTTTTTTTTAGCCCATTCTCCCCCTTTTATTTTTAAAATTTTATTAAACATAGTCATTTTA
    TAATCTATGATAAATTATTATCTAAAGTCTTTGTGAGTATAATTCTGTCAGTGCCTTGTTTCTTTATATG
    TTTTACTAGGTTTTATTGTGAGCCACTCATTTTCCCTGGAGTTCTTATGTGTTGGAATTCTTTGGGGCCT
    AGGCCAAAGCTGGGTTTTTAAGAAAGGAACCTGCTTTTGCTTTGACAGTTGTCTGGGGACACTCCAGAAT
    CACTTTTTTTTTTTTAAGACAGGGTCTCATTCTTTCTAGGCTGGAGTGCAGTGGTGCTATCACGGCTCCC
    TGCTGGTTTGACCTCGCTCAGTGGCGAGGTGGTGATCTTCCCACCTCAGCCCCTCTCATAGCTGGGACTA
    TAGGCATGTGCCACCATGCCTGACTAATTATTCTGTTTTTGGTAGACCAGCCTGGGCAACATGAGTTTCA
    CTTTACTTGGGTTACTGCTCCCAGCCTGAATCGCTTTTAATTATCTGAATGATTATTCTGGGCTGGGTGT
    GGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTAAGGTGGATCTCACTTGAGGTCAGGAGTTCGA
    GATCAGCCTGGCCAATATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCATTAGCCAGGCGT
    GGTGGCGCACACCTGTAATCCCAGCTACTTGTGAGGCTGAAGCAGGAGAATTGCTTGAACGTGGGAGGTG
    GAGGTTGTAGTGAGCCGAGATCACACCATTGCACTCCAGCTTGGGCTACAGAGTGAGACTCCATCTCAAA
    AAAAAAAAAAAAGGAAAAGAAAAAAAAAGATTATTCTGATTATTCTGATCATGTATGTGGTGTGAATCTT
    GATTGCAAATCCAGATCTGAGGGCTCACTATGGTTATGAATTCTCAAGAAAGAATTATTTTTTTTCCCTC
    CACTGTACCCTCAGGTCATAACAAGCTAGTTTTCTTGCTGTCTCTATCTGCCCGGTAGGTTTAGTGTTCA
    ATCTGACTTCCTTGAGGATCTGACTCTTTGCAGGACCCAGCTTTATTTAGGTGCCTCCTCTTGGACTACT
    CGTCTTGGAAAAAGCTTAGGCTCTACCTCCTGTGCTCTGCATTCCATGCAGCCATCTGGATACAAAGTCA
    TAAATCCCCAGGGATTAATGAAACCTTTAGGGCAAAAGTCAGTTTGTTTACCTCCCAGGGTTGCTACTTA
    CACCTCATTTTTGGCCTATATGAAATTTTACCTTTCATTCTAGTTCACCAGTACATTGTGAAAGATGTTT
    TAAATGTTTTACAGAAGTTTAGATGGTTCAACTGGGAAGATCATATAGGATTTCTATTCTGTCATATTGC
    CAGAAACAGAAGTCATCTACATTTTTTTTCCCTAGTCTTTGGTAGATGAGTGGCTGGATAGCTACAAGCA
    AGACCAGGATGCAGGATTTCTGGAGCTTGTTAACTTTTTCATCCAATCTTGCGGATGTAAAGGTGAGGAA
    ACTGCTCCCCCTTTCTAATTCCCAGCCTTTTGTTCCTATGTTATGAAATTCTTTCTCCATTTAGATAAGT
    AGGATTAGACATTTCCTAAATAAAGGAAGATGGTAAAGCATTAGAAAAATTTTACTTATTTAGGCCGGGC
    ACTGTGACTCACACCTGTAATCCCAGCACTTTGGGAAGCTGAGGCAGGTGGATCACTTGAGGTCAGGAGT
    TTAAAACCAGCTTGGGAAACATGGCGAAACCCCTACTAAAATACAAAAAATTATCTGGGTATGGTGGCTC
    ATGCCTGTAGAATTGCTTGAACCCAGGAGGTGGAGGTTATAGTGAGCCAGGATAGCGCCACTGCACTCCA
    GCCTGGGCGACAGAGCCAGACTGTCTTCAAAAAAAAAGAGAGAAAAGTTTCACTTCTTTAACTAAATTTT
    TTATTGATAAATATGGGAAGTGATCCAGTAGGGAACCATGTTGGTTTAGAGGCAAGACACTTTAGGGAAA
    AAGAAGTAATCACGCTTATGTTATGGCACAAATTTGAAGACACCCTTTGGTCTTTCTGGATGCAATAATG
    TTACTCTTTGTTTCCCAGCTACTTTGCCCATATTTCTCCTCTGTAGCTGTGGTACCCCCAGAACTCTTAT
    TGCCATGGCTTCGTTTATTGGGACAGATGGTCTCAGTCAGGTTGTATTATATAAGAAATCAGCATCTGGC
    TTAGTTTTATTGCTGTCTTTGTTTTGTTTTTTTTTGTTTTTTTTTGAGACAGAGTCTCACTCTGTTGCCC
    AGGCTATAGTGCAGTGGCATGATCTCGGCTCACTGCAACCTCTACATCCTGGATTTAAGCGATTCTCGTG
    CCTCAGCCTCCCAGGTGGCTGGGATTACAGGCGAGTGCCACCATACGTGGCTAATTTTTGTATTTTTAGT
    AGAGATGGGGTTTCACCAGGCTGATCTTGAACTCCTGACCTCAGGCGATCCCCCTGCCTCGACCTCCCAA
    AGTGTTGGTATTACAGGCATGAGCCATCGCGCCTGGCCTGTTTTTTGGTTTGTTTTTTTTTTGTCGTTGT
    TGTTGTTGTTTTTTGGCAGTTTTTCACTGCTTGATTCCTTTTTTTTTTTTTGAAACAAATCCTGCCTCAG
    GTTTATTTGTACAAATAGTGCAAGGGGACACCAGTCCTGACCTCATGCTGGCAGAGAGAGATCTCTACTC
    TGAAGCCTTTGTACAGGCCTGGGTACTTTTGGGAGCCTGAGCTGGAACTGAAGGTGGAGGTGCAGCCTGG
    CCCTTCATTTGATCCTTGGCCTTTGGCTGGCACAGCCTGAGCCCCTTGGCATTGTGGGCACAAGCATGCT
    TCTCAAGCTTGGGGTGGGCAGTGTAGGCAAGTCGGCTTAACCTCCTTGGGCTTTATGAGGGACTTGATAG
    CCTCAGCATGTGCACTCATGGCCCTATGGCCCTGGCATTGTTGCCCTGCATCATCTTTTTTTTTTTTTTT
    TTTGAGACAGTCTCACTTGTTGCCCAGGCTGGAGTGCAGTAGTGCGATCTCAGCTTACTGCAACCTCCGC
    CTCCCAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACCCGCCACTATG
    CCCAGCTCATTTTTTGCATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAATTCC
    TGACCTTGTGATTCACCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGACCGACTGCACCCGGC
    TGGCCTGCATCTTCTTTAGGCCGTTCTTGTTGTGCTTCTTGGCAAAGCACATATTCCTCAGGAATTTGGG
    TCTATCCGCTTAAGAGATTTGTATCTTTGTGATTGGGGTTTCTTGATGCCATTTCTGTGCAGTTTTCAGC
    CTGGTTGTGTGAGGTGTGGTTCTTGGATTTGGCCATGTCTACACCATAACCTGTGGCTCCCTTCCTTTGG
    GTTTTTAGAGAGATTGCCTTATAGCAGATACATGGTTGGATGTTGGCAGACTGTTGACCAGGGGGTCTTC
    TCATTCCCCACCTAAGCTCTTTGCAGAATGTTTCCTTGCTCATCTTCAGGTATATCTGTAAATGATCAAA
    GCATCCTGTTATTTTCCTTGTAAGCACCTCATATCCTTCATTCTTTAGGCATTGTGACCCCTGAGATGTT
    CAAGAAGATGTCCAACTCAGAGATCATCCAGCACCTAACAGAGCAGTTTAATGAGGTGGAAGAAGATGAC
    CAGGATCCTCTTACCCCTCTTATTTCAAAACACAATGCCTATTATCCCCTTCTTTTTGATTCTGTGAAAT
    AGGGTAGCTTTTAGAAGGAGGAATTCAGTCTCTTGGGGGTAAAAGAGATCTGAATATGCCTCTCAAATTG
    GGGAGAATACCAGGAAATGAGGTATAACAGATATTTTTACTGGACTGTTGAGTTTTGACATCCAAGCCCC
    TATGACTTCATGGACCTGGTAATAACTTTCCCATCCTTTTCATACATCCTTTTGTAGGACTCGGGGGACT
    ACCCTCTCATAGCTCCAGGTCCATCCTGGAAGAAGTTCCAGGGCAGCTTCTGTGAATTTGTGAGGACATT
    GGTCTGTCAGTGCCAGTACAGCCTCCTCTATGATGGCTTCCCTATGGACGACCTCATCTCCCTGCTCACT
    GGCCTCTCAGACTCACAAGTCCGCGCCTTCCGTCACACTAGCACCCTGGCTGGTGAGCATTCATTTTTAC
    TCTGGACATTCTCCTGGGGATTTATAGGACTTTCCTCTGTTCTCTGATTCAGGATCTTCTTTCCTACCTG
    CATCTTGGCTCTTCACTTCAAGGCCATGCCTCTTTTATCCTAAACTTCAATCCAGTTTCTCACTAGTGGA
    GTTTTGAGAAGGGGTAGATCACAGCAACAATTTCCAGGTTTAATATTTTTGTCAGAGCTCACCACTAGGG
    GTATGCTGATGGTTAAAAGAAGACCACAAGGGTTCGAGGGAAACAGTCCACTCCAGGACAGGCCGTCTTA
    GGATTTTAGGGTCATCCTGCTCTGACTTCTCATTTTCATTGCCCTTCTTTCCTTCTATTTTTTCCTTCTA
    CTCATCCTCTCTCCTCTGACCTCAGTAATGATTTCTTTATCTCTTTTTCCTTTCTCAAAGCTATGAAACT
    GATGACCTCCCTGGTAAAAGTTGCCCTCCAACTGAGTGTGCACCAAGATAACAATCAGCGTCAGTATGAG
    GCTGAAAGAAACAAGGGGCCAGGGCAGAGGGCACCTGAGCGGCTGGAGAGCCTGTTGGAGAAACGCAAAG
    AGGTGAGGAGTGTTCCCTGCTTCTTCCTTCCCTTTTTCCCAACTTGCACCCACTTCTGCCACAGACCCCC
    TTATCAGGCCCTGGGCAGTCTTTCAAGATCATGAATAATGGAGTCTGGCAATAGTTTCATAGACCCATCC
    TGTAAGGGGTCATTACATGCTCATCTTGTTTCTGGGTTGTGGGCTCAACAAATAATGTGTAATTTAGAGT
    TCTCTCTAATTCCACTTTTGCTCAGTTTTTTTTTTTTTGTCAGCACTCATTGTTAGGAGTGTTTAAGTGC
    TGTACCTGCCTGTCATGCAGGGAATTAAGTTTTGACCTACTTGTCAAGTGTGCAGTGTGCAAGTGAGTGC
    AGGAGTACTAAAAGCCAACCATTGACAAGGGAGGCCAAATTTTCAAGGAATGGAAAGAAAACAGGCCTCC
    CAGTGAACTCGCTTTAAATTCTGTTGAATTTAGTCACTAGCTCCATAGCCTTATGTAACCTCTCTTCAGT
    TTTCTTTTCTTTAAAAAAAGGAATAATCTCACCTTTTTGTGAAGAATAAATGAGATGAGTAAATTGCCTC
    GCTTGGCTCATAGTAGATACTTGTTTTGTTTCCTTTTAAACTTAAAGATGACCACTGGGGGGCATAAGCC
    ATAGCAAAATCTTTTACGCTGATGAAATCTTTAATCAGCTGGGTCTGTCATAAGAGTAACACTACTGGTG
    TTTTCTCCTCACTCTCTGTTAGTGTCTAAGACCCCTCCTATGAGGTTGGCAGGACTCACCATGTCTGACC
    ATATCATTTGGCTGTTTCTCTAGCCCTCACAAAAGATCTCTTCCATTCTAAATCACTAGAGAAGGAAACT
    TTCCACTTCCCTTATTCTCTTTTGTTATTTTGCATTGTCAGGTTGATCTTAAAAGCTTGAATATTAATCT
    ATCCCGGATTTTAAATTATGTACACTAGTGCGAATCTTTTCTTTTCTTTTTCTTTTTTGGCTTCTCTGGG
    CTCTTGCTTAATCAATTATCATTATAATTCCCCCATTTTATCCTAAATTTCTTCCTCCCCACTATTTTCT
    TCTCTCCACTTATAAATGTATATAGGCTCTTGAATCTTTATTTCACTCTGCTCCTTTGTGAGTTACTCTT
    TTTTCCATCCTTCCTTTTAGGATAGTATTTCCTTACTTCCTTAATGTCCAACTAACTGAATCCTTTTTGC
    TACCTAGCTTATTTCCCCATTTTCTTTGAAACTGTTCTTTCTAAACCAATGACTTCCTAATTCCTAAAGC
    CAGTAGCCTTTTCTCAGTCTCCCTTTCCCAAATATTTTACATATTTGCTTTACCTATTTTTGCTATTTTA
    GTGTAAAATATGGACATATAGGTACTTCAGCCCTAAAGACTTAAGTATGTCCTGTCGAAAATGATATGTT
    GTTCTTACATAATTGAAGAGGTGGGGCCTGTTGTCTTAAAGGATGTTCCCTTTCTCCTTATAATTCTGTT
    TCCTTGGTGTCTATTGTTCATGTGATCCAGGATTTTTCAATAATGGCACTATGGACGTTGCGGGGCTGTC
    CTGCCATGGTAGGATGTTTAGCAGATCTCTGGCCTTTATCTACTAGATAGATACCAGTAGTACCCTGCTC
    CCCCAGCAAGTTGTGGCAGCCAAAAAAATCTTTAGACACTGCTAAATCTCCCCTGGGGAACAAAATTAGC
    CTCAGTTGAGAACCACTGATCTAATTCCTTTCTTCTCATTTTTGCTAGTTCCACTTCTATTAAACTGCAA
    CTTACTAAAAATTTGTTTTCCAAATTGTTAGAAACATATACGGGCATCATACCTCAAAGATATTGCAGAT
    TCAATTCCAGACCACCATAATAAAGTGAATATTGAAGTACAGTGAGTCACACAATCAAAAAATCAAAATT
    TTTAATTCTCAGTGCATATGAAAGTTGTGTTTACACTGTAGTCTGTTAAGTGTGCAAGACTATTGTCTAA
    AAAGCAGTGTATGTACCTTAATTTAGAAATATTTTATTGCTAAAAAATGCTAATGACAATCTGAACCTTT
    AGCGAGTTAAATCTTTTTGTTGGTGGAGGGTCTTGCCTCCATGTTGATGGCTGCTGACTGATCAGGGTAG
    TGGTTGCTGAAGGTTAGGGTGGCTGTAGCAATTTCTTGAAATAAGACAACAGTGAAGTTTGCTGCATCTA
    CTGACTCTTCCTTTTACAAAAAATTTCTCTGTAACATGAAATGCTGTTTGATGACATTTTACCCACAGTA
    GAAATTCTTTCAAAACAAGAGTCAATTCTCTCAAGCCCTGGTGCTGCTTTATCAGCTAAATTTAGGTAAT
    AGTCTGAATCATTTGTTGTCATTTCAACAGTATTCACAACCTCTTCACCAGGAATAGATTCCATTTCAGG
    AAACCACTTTCCTTGCTCATTCATAAGAAGCAATTCCTTATTTGTTCAAGGTCATGAAATTGCAGCAATT
    CAGCCACATCTTCAGTCTCTACTTCTAGTTCTCTTGCTATTTCCACTGCACGTGTAGTTTACTTCCTCTA
    CTGAAGTCTTAAACCTCTCAAAGTCATCCGTGAGGGTTAGAATCAACTTAACTCTTGTTAATGTTGATAT
    TTTGACCTCTTCCCATGAACCACAAATGTTCTTTATGGCATTTAAAATGGTGAATCCCGGCCAGGCACAG
    TGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGACTACGTGAGGTGAGGAGTTTGA
    AACCAGCCTGGCCAACATGGCAAAAACCCATCTCTACTAAAAATACAAAAATTAGCTAGGTGTGGTGGTG
    CTGCTTGTAATCCCAGCTACTTGGGAGGCTGAAGCAGGAGAATTGCTTGAACCCAGGAGGCAGAGGTTGC
    AGTGAGCCAAGATTGCGCCACCACACTCTAGCCTGTGCTATGGAGCAAGACTCCATCTCAAAAATAAATA
    AAATAAAATAAGATAAAATGGTGAATCCTTCCCGGACGGGGGGTTGAGTTTTTCTGGTTTTGTTTTTTGT
    TTTGAAGACAGGGTCTCGCTCTGTTGCCTAGACCGGAATGTACTGGTGCAGTCATGGCTCACTGCAGCCC
    CAACCTCCTGTGCTCAAGTGATGTTCCTGCCTTATCCTTCCAAGTATCTGGTACTACAGATTGCATACCA
    CATCTGGCTAGTTTTTGTTTTGTTTCTGTAGAGACAGGGTCTCACTGTGTTGCCCAGGCTAGTCTTGAAC
    TCCTGGGCTTAATCAGTCCTCCCACCTCAGCCTCCCAAAAAGTTGGGATAGTAGGCATGAGCCACCATGC
    CTAACCCCAGGAGGTTTTAAATGGACTTTGCCCAAATCATCAGAGGAATCACTGTGGCAGCTATAGCCTT
    ATGAAATGTATTTCTGAAATAATAAGACTTGAAGTTGAAATTACTCCTTGTTTCATGGGCTGCAAAATGG
    ATGTTGTGTTAGTGGGCATGAAAAAAGCAGCATGGATCTCCTTGTACATCTTCACCAGAGCTCTTGGGTG
    ACCAGGTGCATTGTCATTGAGCGGTAATATTTTGAAAGGAATCTTTTTTTCTGAGCAGTAGGCCTCACCC
    ATGTGCTTAAAATATTCAGGAAACCATACTGTGAACAGATGTGATATCAGTTAGGCTTTGTTGTTTCATT
    TATAGAGCATAGGAAGAGTAGATTTAGCATAATTCTTAAGGGCCTTAGGATTTTTAAAATGGTAAATGAG
    TATTGGCTTCAACTTAAAGTCAGCAACTGCATTAGCCCCTAACGAGGAGTCAGGCTGTCCGTTGAAGCTT
    TGAAGCCAGGCATTGACTTCTCCTCAATAGCTGCGAAAATCCTAGATTTTCCAATATAAGGCGTTTTCCA
    ATTATAAGGCAGTTTAGTCCACATTGAAAATTCATTGTTTAGTGTAGCCACCTTCATCAGTGATCTTAGC
    TAGATTTTCTTGATAACTTGTTGCTTCTCTATCAGTATTTGCTGCTTCACCGTGTACTTTTATGTTATGG
    AGAGGGCTTCTTTCCTTAAACCTCACGAACTAACTTCTAGCTTCAAACTTTTCTTCTGTGGCTTCCTTAC
    TTTGCTCAGCCTTCATAGAATGGAAAACAGCTAGGACATTGATCTGGATTGGGCTTTGGCCAAAGGGAAT
    GTTGTGGCTGGTTTGATCTTCTGTTCGGACTACTAAAACTGTCTCTGTATCAGCAATAAGACTGTTTAAC
    TTTCTTACCCTTTGTGTGTTCACTGGAGTAGCATTTTTAATTTCTTTCAAGAATTTTTCTTTTGCATTCA
    TAACTTGGCTGTTTGGCACAAGAGGCCTAGCTATCGATTGGCCTGTCTTGGCTTTCAACTAAGCTTAATC
    ATTTCTTTTTTTTTTTTTTTTTTTTCTTTTTCAGACGGAGTCTCACTTTGTTGCCCAGGCTAGAGTGCGA
    TGGCGTGATCTTGGCTCACCACAACCTCCATCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGA
    GTAGGTGGGGTTATAGGTGCCCGCCACCGCACCCAGCTAATTTTCTGTTTTTAGTAGAGACGGGGTTTCG
    CCATGTTGGTCAGGCTGGTCTCGAACTGACCTCAAGTGATTTGCCCACCTTGGCCACCCAAAGTGCTGGG
    ATTATAGGTGTGAGCCACTGCACCTGGCCTCTAAGCTTAATCATTTCTAGCTTTTGATTTAAAGTGAGAA
    ACATGTGACTCTTCCTTTCATTTGGACACTTAAAAGGGTTATTAATTGACCTAATTACAATATTTTTGTG
    TCTCAAGGAATGAATAGGGAGGCCTGAGGAGAGAGAGAGAGACAGAGAAACTCTTGTTGGTAGAGTAGTC
    AGAACACAGACATTGTTAAGTTTGCCATCTTATATGGGCACGGTTTGTGGTACCCCAAAACAGTTACAGT
    AGTAACATTAGGCTGGGTGTGATGGCCCATGCCTGTAATCCCAGCACTTTGGGAGAGTGAGGTGGAAGGA
    TCGCTTGAGCCCAGGAGTTCAAGACCAGCCTGGGCAAACATAGGGAGAGCCCGTCTCTACAAAAAAAAAA
    ATTTTTTTTAACTAGCTGGGTGTGGCAGCATGCATCTGGACTCCCAGCTAGTTGGGAGGCTGAGGCAGGA
    GGATTGCTCGAGTCCAGGAGGTGGAGGCTGCCGTGAGCCATGGTCACACCACTGTACTCCAGCCTGGGTG
    CCAGAGTGAGACCCTGTCTCATAAAAAAATATGTAACATTAAAGATTACTGATCACAGATAACCATAACA
    GATACCATAATGAAAAGGTTTGAAATATTGTGAGAATTACCAAAATGTGCCACAGGGACACAAAGTGAGG
    ATATACTATTAGAAAAATGGCACTGATAGGTATGCTCAATGCAGGGTTGTCACACACCTTTAGTTTGTTT
    GAAAAAAATCCACAATGTCTGCAAATCAATAAAGTGCAATAAAGCGAGATACACCTGTATAATGCCAGCT
    AGGGCTTCTGAATAGTCCGAATAACCACATGCAGAGGGGTTATTATTTTTACTAAACACAAACCTCTGAG
    GCATTAGTTGACTATACAGCTTCTTCCAGAATTAGAAGGTTTAAAAGAGAGCATAGGATTACGGGGGTTC
    ACACTATCCTATAATAAACAGGAAGTAGGTGGAAGTTGTAGTTTTTCTGTATCTTCAGGGCCTTATGCTT
    GTTAGGGTACAAGAAAGATTAACCCGTTTCTCCCTGTCCTCCAGCTCCAAGAGCATCAAGAGGAGATTGA
    GGGGATGATGAATGCCCTCTTCAGGGGTGTCTTTGTTCATCGGTACAGGTGAGCTAATGGGCCTCTCTCA
    TTGAAGCAGCTGGCCTTTGGTTATGGAATCTGAGGAAGTGAATAAGGTTTTCCCCCCTCCAACCCTTAGT
    TATTTGGTTAGTCCCAGAATTAATGCAGCTGGAGCAAAAATTCTTGACAACAAATGTTTCAAATTGGTGA
    GGTTTAGTTTATCACCACCTACAAAGGATCTTGTAAGTGCTTCTGGGAGAAAATAAGAGAATAAAGCATT
    GCTGAGTATTTTCCTGCTCTGGCTTTGCCCCAGATCTGCCCCAACTCCTCATCTTTCTGGGGTTGGAGAC
    TTGAGGATAAGAGCAGAGAATAGAAAGGCGGGGCTTTTCTGAGGGATGCCTGCTGCCTGGTGAGCCTTCT
    CCTGGCCCAGCCTGCTGTCTCACTGTGCGAAGGGCAGGGGAGTTTTATACTGTTTAATGACCTGATCAAA
    TTAAGTTAAAAAGAATATATGTAGGCCGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC
    CAAGGCAGGCAGATCACCTGAGGTCAGGAGTTCACAACCAGCCTGACCAACATGGAGAAACTCCATCTCT
    ACTGAAAATACAAAATTAGCCGGGTGTGGTGGCGCATCCCTGTAATCCCAGCTGCTCAGGAGGCTGAGGC
    AGGAGAATCGCTTGAACCCAGGAGGTGGAGGTTGCGGTGAGCTGAGATGACGCCATTGCACTCCAGCCTA
    GGCAACAAGAGTGAAACTCCATCTCAAAAAAAGAAAAAAAAGAAAAAAAAAATTGTAGATGTTTCTCACT
    CTTACCTAGGTTTCACTTCTGCCTCTCCAGAGCCTTTCAGCTGATGTAACCTTTTCTTAATGTGGAATTA
    AAACACACTCACACACTTTGGCCAACAGTTGAATTAACTTTTTTTTTTTTGGCAGGGACGGAGTCTCACT
    GTGTTGCCCAGGCTGGAGTGCAGTGGTGCGATCTCGGCTCACGGCAAGCCCCACCCCCCGGGTTTAGGCG
    ATTCTTCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCGCCCACCACCATGCTTGGCTAATTTTTGG
    CCACCATGCCCAGCCTGAATGAACTTTCAAAATTGGTTTTTAAAAGAGGTATGGAGACCGGGTGCAGTGG
    CTCACACCTGTAACCCCAGCACTTTGGGAGGCCAAGACAGGCAGATCACTTGAGGTCAGGAGTTCGAGAC
    CAGCCTGGCCAACATGGCAAAACCCCGTCTCTACTAAAAATACAAAAAGGAAGCTGGGCGTGGTGGTGCA
    CCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCACTTGAACCCGAGAGGTAGAGGTTGCAGT
    GAGCTGAGATTGTGCCGCTGCACTCCAGCCTGGGCAACAGGTGGAGTGGAGAATTAGGCTGTACAAGGCT
    GGTGTTTAACTATTTTATGGTTTGGTAACAGAAAGAAAATATTTACTAACTGGGTATGGCACATTTTTGT
    AGTCCTAGCTACTTGAGAGGCCAAAGTGGGAAGATGACTTGAACCCAGGAGTTCAAGGCCAGCTTGGGCA
    ACATAGCAAGAGATCCTATCCCTTAAAAAAAATATTTACATGTATTTTACTATATATATAGTATTTACAT
    CGGGAGGAAGAAATGAAAGGCATGGGGTACATGGCATTTCAAGAAAATAAATTCTTCCATGAGAGGGAGT
    TATCTGGTAGGATGGAAAGTGAGTTTGGAGAGAGGGTGGCTCTAAGAGTCAACTTATCTGTCTGTGTCTA
    GGGATGTCCTTCCTGAGATCCGTGCTATCTGCATTGAGGAAATTGGGTGTTGGATGCAAAGCTACAGCAC
    GTCTTTCCTCACCGACAGCTATTTAAAATATATTGGTTGGACTCTGCATGATAAGGTGGGATTCGAGTCA
    GTTTCCCTTTCCGTTTCCTTCATCTTTTTCCTGTCCTAGGACATTTCACCTTTTCCTGGGCCTGCCACTG
    AGATATTTTACCTAGTGACCAATGATGAACCCATCAAGGCGCTGAACCTTGAAATTCCCCTAGCACTGGG
    GAGAGTAGGATAAGGTAGAGAGAGACATACCCAGGAGATGAAATGACTCAAAACCTGAGGTACGGGGAGA
    GAGACTTTCCCATGGAAGAGAGTTATTGATGGATGGTAATGCAGGTGCTAAGGGGCAGCCAAAGGATCCT
    TCTCATCATGAGTACTGCTGTGATCCTTTTGTTTTTTAAGTAAGAAGACAGCTGTTCATTGTTTCTGACA
    TCTTAAGAGTCCTCAGTAGAGGGGACACCCAAGCTAGAATGAGGGATCGGAGAGGGGAGTCTGGAGGCTT
    AGTAGGGGCGAGTAGAGTGTGGTTAGTCTTATTTCCATTCTCCTGGTTTTCCCTCCTCACCAGCACCGAG
    AAGTCCGCCTGAAGTGTGTGAAGGCCCTGAAAGGGCTGTACGGTAACCGGGACCTGACCACACGCCTGGA
    GCTCTTCACCAGCCGCTTCAAGGTAAAATGGGTGGTGCTCCATGGCTTGGCTCTTTGTCGTGGTTCCTAT
    TTCCCCACCTTGTATCTTTCTACCCCTCATGCTCTAAGATGTCTTGGCTATCCCAGCATCTGCCTCTACG
    TAGGCACTCTCTTTAGGAGTCTCTGATTCTAAGAGAACAGAATGTTTGGAATGGGTGTAATGAGATATTG
    AGTGACTTTCTCCTTTCTGCAGGACCGGATGGTTTCCATGGTCATGGACAGAGAGTATGATGTGGCAGTG
    GAGGCTGTCAGATTACTGATACTTATCCTTAAGTGAGTCCTGGGAAGAGGGGAGGCCAGTGTCTCAGACC
    TTTGCCCTTCCAGGGTCATCTCCCTCCACCTGTCATAGCTGACTCTTCCATCTGTGCAGGTTGACTGAGG
    TCATTCCTGAGTTGCAGTATGTTGAGGGGGTAATATTTCTGTCTTCTCTAACTCCCCATACTCCTTTGTC
    TTCCGCTCTTCATTTAGAAGTTTTTTGTGAGTTATGTCCTTGTTGCTTTTGCCTCTTTTTGTTTCTAGCC
    TTGGTTGTGCCAGAAGACAATGTCCCTATTCACACACTCTTTCTGCTTTTCTGTGGGCAGGAACATGGAA
    GGGGTGCTGACGGACGCGGATTGTGAGAGCGTCTACCCCGTTGTGTATGCCTCTCATCGAGGCCTGGCCT
    CTGCCGCAGGCGAATTTCTGTACTGGAAGTGAGTGGGGCTCCTTTTATGTTTCTTTAACACCACGCTCTC
    GGTTTCTCTCCTCCATCTGCTGCTGCCCTACCTAAGGCTTCCCCATCTCTGTGAAAGTCTCCCTGGTGTC
    TCCTTTCTTCTCGCAGCTCCTTGGGGCTTTCCTTAGCTCTCACCTCCTTTTCCTTTATCATCTCCTTGGG
    CCATCTCCTCCCTCTGTGGTCGTTACACCTCCTTGTTTCCTGTTGTGCTGAGCCCTTTCCTCGTGTCCAT
    TCCACCAGACTCTTCTACCCTGAGTGCGAGATAAGAATGATGGGTGGAAGAGAGCAACGCCAGAGCCCAG
    GCGCCCAGAGGACTTTCTTCCAGCTTCTGCTGTCCTTCTTTGTGGAGAGCGAGGTGACATACACAGAGAG
    AAGTCTGGCTGTTGTGCATAGGACCTACAAGTGGGCTGGGGTTGGTGGCTCACGCCTGTAAGCCCAGCAC
    TTTGGGAGGCTGAGGTGGGAGGATCCTTTGAGCCCAGGAGTTTGAGACCAGCCTGGGCAACACAGTGAGA
    CCCTGTCTCTATCGAAAAAAAAAAAAAGACAAATGGAGAAGGATGGGAGTGGACACTGTAGGAAGGAGAG
    CGATATTTAATCAGTAACCACTTCGTAGGGTATTTTTAACATGCTATCATTAACTCCCCATGCACGTTCT
    CCCCTAGCTCCATGACCACGCTGCTTACTTAGTAGACAGTCTGTGGGACTGTGCAGGGGCTCGGCTGAAG
    GACTGGGAGGGTCTGACAAGCCTGCTGCTGGAGAAGGACCAGAGTACGTGTCACACGGAGCCAGGGACAG
    GGACCTTCCACCCGCTAGGGTGAAACTGGGAGGGATTGCTTGCTTCACTTGTACAAGGCAGCAACGGTGG
    CATCGGGTGGGGGGAGCTTGGAGTTGGAAGGTGGCTAATCTTTGATTCTATGTTTTTGATCCTCCTGGCA
    CTCCAGACCTGGGTGATGTGCAGGAGAGCACACTGATAGAAATCCTTGTGTCCAGTGCCCGGCAAGCTTC
    AGAGGGGCACCCGCCTGTGGGCCGGGTCACTGGGAGGAAGGTATGGTGTGAGGGTAGAGTGGGTAGGTCA
    GCAATACTCAGTCTTGACAGGCAATGTGCATCCTTATCCCCCAGGCTGGGGTTAGGGTGCTCCTTCTACC
    CTTTAAAGAATTTCATTTCCAGAAAAAAGGGCCAGGCGCAGTGGCTTACGCCTATAATCCCAGCACTTTG
    GGAGGCTGAGGCAGGCAGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTGACACAGTGAAACCCTGT
    CTCTACTAAAAATACAAAAAAAAAAAAAAAAAAATTAGCTGGGCGTGGTAGCGGGCACCTGTAGTCCCAG
    CTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCTGGGAGGTGGAGCTTGCAGTGAGCCAAGATCAC
    GCCACTGCACTCCAGCCTGGGTGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAGGAGTTTC
    ATTTCCAGAAAAAAGAAATCTCGTGGGAGCTACTGAGTTCTCTTTTCCTTCTAGGCAGATAAGGTCATGG
    GGAGGAGGGCTGCACACACCCCCTGCACCAGTGTTTCTTTACACCTCCCCCACCCCCAAGTGACTCTCAT
    TCCAGGGCTTAACCTCTAAGGAGCGCAAGACCCAAGCCGATGACAGGGTGAAGTTGACTGAGCACCTCAT
    CCCCCTGCTGCCCCAGCTCCTGGCCAAGGTACCGCTGCCCCTCCACTCTGCATCACACCAAGAGAAGTGA
    AAGGAAATGGCCTCTGTGGGGGTGGGAGTAGGAATTAGCAATGTGTCAGCTTGTAAGGAGGCCTCCCTGT
    CAATCATCACAGTTCTCAGCTGATGCAGAGAAGGTCACTCCCCTGCTCCAGCTTCTCAGCTGCTTTGACC
    TCCACATCTACTGCACTGGGCGCTTGGAGAAGGTAGGGAGATAGATTTCCTATACCTTGCTGCTTGCCTG
    CCAGGGCCAAAGGGTTCTATTGCCAGTATCTTTTTTTCCTAAGAACTTGGGTTCCAGAAAAATCAGCAAG
    CCTTTTCTTAGGCATCTTATCCTTGAAGTTTAATGCTTTTAACCCCGTTCCACTTCCCGCCAGAAGAGTG
    TCCTCCTCCCATCCCCACAGCACACCATCTTCTGCCTTTTCTTCTCAGCACCTGGAGCTGTTCCTGCAGC
    AACTCCAGGAGGTGGTGGTGAAGCATGCAGAGCCAGCGGTGCTTGAGGCTGGGGCGCATGCCCTCTACCT
    GCTCTGTAATCCCGAATTCACTTTCTTCAGCCGGGCGGACTTTGCCCGCAGCCAGCTAGTAGATTTGCTG
    ACTGACCGCTTCCAGCAGGAGCTTGAAGAGCTGTTACAGGTAGGAGCTGGGGCTGGACAATGGGACACCC
    CAAACAAGGGTGGGAGGGGCTGCAAGACGGGGGATGTGGACTGATGAGTTCTGGGGGAAATGCTATTGTG
    GATCTTCTTTCCTTCTCAGTCGTCCTTCCTAGATGAGGATGAGGTATATAATCTGGCAGCCACTCTGAAA
    CGCCTCTCTGCCTTCTACAAGTGAGTGGCTTTCCTCCTCTTCCCCATCCCGTTTTTACTGGTGTCTGTGG
    AGTTGGTTCCCTCTGTTCTTTTCCAGTATAACATTCCCCTTTCTCCCCCAAAGCACTCATGACCTGACTC
    GCTGGGAGCTCTATGAGCCATGTTGCCAACTCCTGCAGAAGGCTGTGGACACAGGAGAGGTTCCTCACCA
    GGTGAGTGGACAGGCTAGAGATGGGTTGGGGGCTGGGGGGCTAGATTTTAAGGACCTACGTTCTAGGTGG
    CCACTAGGTGTGTCAAGTCTCAGTGCGTGGAGTGGACAAAGCGAGGAAGATCAGGTGGGTGGGGGTCACC
    TCTTACATTCCTCTCTTAGAAGGTGGACTCTACTTCCAGGGTCTTCTTGGATTTCCTGGAAGCCAAGGAT
    AATATTGAGGGATTCTAGAAGACGTAGAGTAAATTAGGGAGCGGTGACATTTCTACTTCGTGTAGATGCC
    TCTGAAGAATGTCCAGAATATCACTCATTTTCCACTGGAAAAGGAATTAGCTCTCCCTTTACTCATTTTC
    TCTCCTCTTCACTGGTGTGTTTCTGGCTATCTGTCTCTCCCTCTCCTAACCCAAACCTCATTTTTCAAAC
    CCTTTGTTGTTACAGGTTATCCTGCCAGCCTTGACTCTTGTCTATTTTTCCATTCTCTGGACACTAACCC
    ACATTTCTAAATCAGATGCTTCCCAGGTGAGTGTGGGTCTTAGATGGGATAATGGGAACAGAGGAGTCTG
    TGTCTTCATTCTTCCCCTTCAAGCCACTGTGCCCACAGAAGCAGCTGTCGAGTTTGAGGGACAGAATGGT
    GGCCTTCTGTGAACTCTGCCAGAGTTGCCTCTCAGATGTGGATACTGAGATCCAGGAGCAGGTGAGTACT
    GACTCAAGTGGGAGCAACAAGGCGAGTATCCCTGCCCCCATTCCAAGGAGAAATTGACAGTGTCATTTCT
    ACCTTGGGAAACATTCTGTCCTTTTAAACATCCTTTCTTTTCCCCCTACAGGCTTTTGTCTTATTAAGTG
    ATCTACTTCTCATCTTTAGCCCTCAGATGATAGTTGGGGGCCGTGATTTCCTTAGGCCACTTGTCTTTTT
    TCCTGAAGCTACTCTCCAGTCTGAGCTAGCCAGCTTCCTCATGGACCACGTCTTCATCCAGCCGGGAGAC
    CTGGGCAGTGGTGCAGTGACTCTATACCTGGGGCTCAGTAAGGGCTGGGGCTTGGGTGTAGCTCAGAAAT
    ATAGGGCAGGAAGCTTAAGTCTGTGATTCCCTTTTGCCTTCCATGTGAGCCAGGTGATTCCCAGGAGGAT
    CATTTACAGATAGAGCGGCTACACCAGCGGCGCCGCCTCCTAGCCGGGTTCTGCAAGCTGTTGCTTTATG
    GGGTGCTGGAGATGGATGCAGCCTCAGATGTTTTCAAACACTACAACAAGGTACACCAAGGCCCTACAGA
    AATAAAAGAGAAGGGAGCAAGTTGAGCACAGCCATGACTTGGAAACATAATAGCACTACAGGTGGGGGAT
    ATCCAATGGTTTCAAGCTTAAAGGAGAAGTAGGAGGGAAAGGCACTGGGAGTTCAGAATAAGGGGACAAA
    GAAAAGAGACTTAATAGTAAATAATTATGGAGAAAAAGCTGTGAAGAAATTCACTGGAGGGGAAATAGCT
    TATTCTTCCTGTCCAGAAATATTTATTTTTCTCTGACCCCAGTCTATGGAATGGGGTAACATAAGAAGGA
    AAAAAGAATAAAAGAGGGATCAGTCCTCACAGGATACCTAGATAGGTGCCCCAGTTTCCTCAACCCACCA
    GTATTGATTTATGCTTATTGAGCACCTGCTGTGTGCCAAGCACCATTCCAGGAGCTAAGGCCACAGCAGT
    GAACAAAACACACAAGTTCCTATTTGGTGGAGCTTCCATTGTAGTTGGGGAGAGACAAATAGTGAAGGTG
    TCAGTGCTGTGTGAGAGGGCACTGTCAGGCTTACTTCTCTTCTCCAAGAATTGGGCTTGGGGCAGTGTTT
    ATTTCTTTTCTTTTCTTTTTTTTTTTTTTCCTGAGACAGAGTCTAGCTCTGTCGCCAGGCTAGAATGCAA
    TGGCACTATCTCGGCTCACTGCAACCTCTGCCTCCCGGGGTTCAAGCAGTTCTCCTGCCTCAGCCTCCCA
    AGTAGCTGGGATTACAGGCATGCACTGCCACGCCCAGCTAATTTTTATAGTTTTAGTAAAGACAGGGTTT
    CACCATCGCCAGGATGGTCTTGATCTCCTGACCTTGTGATCCGCCCAACTTGGCCTCCCAAACTGCTGGG
    ATTATAGGCGTGAGCCACCATGCCTGGCCTGTTTCATTATTTGTTTTTTATTTTATTTATTTATTTATTT
    ATTTATTTTTTGAGACGGAATCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACT
    ACAAGGTCCGGCTCCTGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCAC
    CCACCACCACGCCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCGTATTAGCCAGGATGG
    TCTCGATCTCCTGACCTCGTGATCCACCCGTCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCA
    CTGCACCCGGCCTTATTATTTGTTTACCGAAACTCTGATTTATTTTGGGCTTTGCACCTGTTGATTAGAG
    ATGAAGTCTAGTCTGAATTATTTTATGCTTCAGTTCATGTTTCCTACCTAAGGGAGTTTGGGAGGGAGAC
    ATGATTAGGGATGTGAAGGAAGAAACCAAATGGTCTTTTCCACCAGTCAGACATTACCTTCCCCACTCTT
    TCCCCTCAGTTCTACAATGACTATGGTGACATTATCAAGGAAACATTAACTAGAGCAAGGCAGATTGACC
    GAAGTCATTGTTCCCGAATCCTGCTGCTGAGCCTCAAGCAGGTGCGCCCTTCTGCCTTGAGGACATGCCA
    GCCCTGTTGTCCCCAAGTCTCTGTCTACCCTCAGCCACTCAGAATGTAAGATCCTTCAGAGCAGTAACTT
    TTCTTGTTTTGACTCTTAACTTTACGTGATAGGTTCCTTCATTCTTAGTAGACCCTTGGGAAGTATTTTT
    TTTTTAACTGACTGGGCAACCCCATCAAAAGTAACTTATCAGGACATTGGAACGCCAACAAGGAAGTGAT
    AGGGAGTTCCTAATTATTTTTCAGTCTGTTCTCTCTCAGCCTCCTTCAGTTACTATAGACGTTTTTATTT
    AATGTTATGGAGTAGAGGTGATTGGCTTTGTTTGCTGCAGGGGTATCGGGAGTTCCCTAGAAGGAAGGAA
    AGAGTAAAAGTAGTAGATGGTGTCCCAAGGCCTTTGGAATTTCTGAGTAGAGAAGAGAATGCTGGACTTC
    TCTGTTTCCGCCGTGTTCCTCCCTTTCCCACATGCACCATGTCTCCTGGACACAGCTGTACACAGAACTG
    CTGCAGGAGCATGGGCCCCAGGGCCTGAATGAGCTTCCTGCCTTCATCGAGATGAGGGACCTGGCCCGGA
    GGTTTGCCTTGAGTTTTGGACCCCAGCAGCTGCAGAACCGTGACCTCGTGGTCATGCTACACAAGTAGGA
    AGTATTGGTTGCAGGTTGTGTCCAGGGAGGGCTGGGAACGAGGTCTTGGAGGGAGGTCTCGGAGGGAGGG
    TATGTGTGTCAAGGCATAGCAGTCAGGTTAGGGGTGGGTATGCCTTTGGAGACAAGCTGGGGACGGGGGG
    AGGGTGCATTTGGACAGGATAGCTCAGGCCTGGGAGGGAAGTGGGAAGAGACTTTCTTCCTAAAATAATT
    CTGCCCAACCAAGGGAAGGCATCCAGTTCTCCTTGTCTGAGCTTCCTCCAGCTGGCTCCTCCAATCAGCC
    TCCAAATCTGGCATTCCTGGAGCTCCTTTCAGAGTTTTCCCCCCGACTCTTCCATCAGGACAAGCAGCTT
    TTGTAAGTTGGTGGGTGGATAGAGATGTGGATTAGGGAAGGGCCTGCTGAGGGCCCACTAGCCCCTTCAG
    GCTTTTGGTTCTACCTCTTTCATAGACTGTCCTATCTAGAAAAGTGCCTGCAGCATGTCTCCCAGGCACC
    TGGCCATCCCTGGGGCCCAGTCACCACCTACTGCCACTCCCTCAGCCCTGTGGAGAACACAGCAGAGACC
    AGCCCTCAGGTCCTCCCCAGCTCCAAGAGGAGGCGCGTTGAAGGTAGGGTGCTGTGTGTGGGTATGGGGG
    TGCCCAGCAGGTGGTCGTTGGCTGCCTGCAAACTTATTTTGTCAAGTATTTCATTGAGCATCTACTGTCA
    TTCAGGGTATTAATTTCTTTCAAGGTTTATCTAGTCAACTGAAAACCTGTATTAAAAATCAAATAGAGGG
    CCAGGTGTGGTGGCTCATGCCTGCAATCCCAGCACTTTGGGAGGCCGAGGCAGGCGGCTCACGAGGTCAG
    GTGTTCGAGACCAGCCTGGCCAAGATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCTGGGCA
    TGGTGGCGCGCATCTGTAATCCCAACTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAACCCGGGAGGC
    GGAGGTTGCCGTGAGCTGAGATTGCGCCACTGCACCCCAGCCTGGGTGACAGAGCGAGACTCTGTCTCAA
    AAAAAAAAAAAAGAAACTGATGATAAAACCAAATATTGAACTAAGAAATCTATACATTCAGAGTACCCTC
    CTCAACATCTTGTATTCCATTATTTTCGAAACCTTGGACACTTTTACACAACCTTCATTGTTTTTTACCT
    TCAGATCACCTGTTGTCTTATAAGTTGCCTAAGGTTTTGTTTTTGTTTTTGTTTATTTTTTGAGATGGAG
    TCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCGCCATCTCGGCTCACTGCAAGCTCTGCCTCCCAGGT
    TCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCACCCGCCACCACGCCTGGCTAA
    TTTTTTTTGAATTTTTGGTAGAGATGGGTTTTCAGCATGTTAGCCAGGATGGTCTTGATCTCCTGACCTC
    ATGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGTGCCTGGCTGCCTAA
    GGTTTTTTCTTTGCTTTTTAAAAAACAGCTTCATATTAAGCAACATTGTAGCAATCATTATTTCATATGC
    AAGTATGTGTTTTCTAGTGTACATTTTTAAATGGGACTTTATGTGACAGCTTTATTGAGATACAACTTAC
    ATATCATAAAAATCATTTTGTTTTTTTTGAGACGGAATCTTGCTCTGTCACCCAGGCTGGAGTCCAGTGG
    CGTGATCTCGACTCACTGCAACCTCCGCCTCCCACGTTCAAGTGGTTCTCCTCCCTCAGCCTCCTGAGTA
    GTTGGGATTACAGGCGTCCACCACCACCCCCAGCCAATTTTTGTATTTCTAGTAGAGACAGGGTTTCACC
    ATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTTGTGATCTGCCCACCTCAGCCTCCCAAAGTGCTCCCA
    AAGTGCTGGTGAGCCCCCGTGCCCAGCCAAAAATCACCCTTTTAAAGTGTACACTTAGCATATTCACTGA
    ATTGTACAACCATCACAACTATCTAATTTTAGAACATTTTCATCTTTCCAAAAGAAACCACATACCCATT
    CGCAGTCTCTGCCCATCTTCCCAGCCTTAGGTAGTATCACTAATATAATTCCTATCTCTTGATTTGCCTA
    CTCTGGACCTTTCATATAAATGGAATCATATAATATGTGGCCTTTTATGTCCAGCTTCTTTCACTTAGTA
    CAGTGTTTTCAACGTTCATCCATACTTTGACATATATCAGTATCTCATACCTTTTTATGGCCGAAACAAT
    ATGCCATCGTGTGGATATGCCACATTCTACTTATCCACTTGTCAGTTGATTGACATTTGGGCTCTTTTAT
    GCATAATGCTGCTAAACATGCATGTACACATTTTTGGGTGGATGCATCTTTTCAGTTATCTTGAGATATA
    CCTGGGAGTGGAATTGCTGGTTCGTATGGCAATTCTATGTTTAAGTTTTTGAGGAACTACCACACTGTTT
    TCCAAAGTACCTACACCAGTTTACAATCCTACCAGCAATGTATGGGGACTCTGGTTTCTCCATGTTGTCA
    CCGGTACTTATTAATGTCTTTTTTTATTTTATCCATCTCCTAGTAGCTGTGAGGTGTGGTATCTCATTGT
    GGTTTAAATTTGCATTTCCCTAATGACAAATGGTATTGAGCATCTTTTCATGTGCTTATTGACAATTTGT
    TTATCTTCTTTGGAGAAATGCCTGTTCTGATCCTTTAGCTATTTTAAAATAGGGTTGTCTTTTTATTGTT
    GAGTTGTAAGTATTCTTTATATATTCTAGACATGAGATTCCTTATGATATGCAAACATTTTCTCCCATTT
    TTTGGGTTGTCTTTTCGTGTTATTGGTGGTGTCCTTTGAAACACGAAAGTTCTTGAATTTGGTGAAATCT
    AACTTTTTCCTTTTGTTGCTTGTGCTTTTGGTGCCATATCTTGAGAAATTGTTGGCTAATTCATGAGGTC
    ACAAAGATTTACTCCTGTGTTTTCTTCTAAGAGTTATAGTTTTAGCCGGGCATGGTGGCTCACGCCTGTA
    ATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACA
    CGGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGCCAGGCATGATGGTGGGCGCCTGTAGTCCCA
    GCCACTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATTG
    TTCCACTGCACTCCAACCTGGGCGAAAGAGCGAGATACTGTCTCAGAAAAAAAAAAAAAGTTATAGTTTT
    AGCTCTTAATTTAGATTTGTGATCCAGTTTGAGTTAATTTTTGTATATAGTGTGAGATGAGGTGTGATTT
    CCTTTTGTTGTCTTCTGTGTACCACCTAAAATTATGTCTTATACCACCAGGAAACTCTGCTTTAATTAAT
    TAACTAATACATAGTTGTTAGGGACCTACTATATGAAAGATACTATGTTCAGTGTTTTAGGGGCCATGAA
    GAATCCAGCAGCTTATTATCTAATAGGAAAGGGAGAAATTTTAATTATGGAAAACTCGAATTATGAACAA
    TTGCCAATAACTGCCTGAGGGCTTGTACAAGTGAATGCTGTAGAATTCAGAGAATGATGAGAGCTTTACC
    AAGAAGATGGAATTGCTGGAGCTTAAAAAATAAGTAAGATTTTTATAAGCTGAACAGAGGAAGGAGGAGC
    ATCATGGGCTGGCAGAATAGCACAAGCAAAGCATAGAAGGCTGTGTGCTGGGCGAAGCATGGAGGTACAT
    AGATCGCTCTGCCTAAAGCGTAGGGTTTGTGAAGGCAAGATAAAACTGAAGAGTTTGTTTTTATTCATTC
    AATAGATACTGAGCACCGTGTGCTGGGAATACAGCAGTGAACAAAAAAGCTAAACATCTCTTTCTTTAGA
    GAGTGTCAGATAGTAGTAAATACTATGGCAAAAAAGCAGGGAGGATTGTGAGGAATGCTGGCGGGGTGGG
    GATTGCAGTCTTACAAAGACTGATCAAGGGAAGACTCAACACAAATGTTGGGCAAAAAGCCTAAAAAATA
    AGTTAAAAGATACACTTAGGCACATTACAATTTTAACAGGTTTATTTGAGCATTCAGCAATTTTATCAAT
    CAGGCAGTGGCAGACCGCCAGCAGTTCAGGGCGCCACCACCGAGGAAACCAGAGGGGACACTTACAAGGT
    GTCTCTGGGAGCAAGACAAAGAAACTATTTGATTGGTTAGAGTGGAAAGTTCATATTTAGAGGTTAACTG
    ACCATAAATCTCTTGTTAGAGGTTAGTCGGTAGTTTCTGACTGGTTAAGCTGAAGTTTCCTGCTCCTAGG
    TTACACACAACACTTTCACTCTGAGTTGAGTTTCAGTTTGCTGACTTAGGAACCCAAAGTGCAGGAGCCA
    TTTCAGCCTATTGGCCTCCCAGCGAATTTTTTATAACAGGTGAGGGAGGGTGCTTAGTGTGTATCAGAAT
    AGCATACCAAAGGAAGCAGCCAGTCCTGAGGCCACGAGGGGATGGGGGGAATGTTCCTGGAATGTTCAAG
    GAACAGCAGAGAAGACCATTATGGCCAGAATGCAGATTGTAGGACATGAGACCAAAGAGGGTACAGGATC
    CCAGAGCATCTAAGGCAGAGACTGGCTTTTACTCTGAGTGCAGTAGGAAAAGCATTGGAGGATTTTGAGG
    AACGACAGGATCAACCTCTGTTTTTAAAGGATCCTTCTGGCTGCTGTGTCGAAAACGACAGGAGATGGGG
    GAAGGCTAGAAGCTGGGAGACCAGCAGGGAAGTTGTGGCTGTTGTAGGAGTCCAGCCCAAGATGCAAGAT
    AAGAGAGCTTCTTAGGCCGGGGGCAATGGTGGAGGTGCCGAGAAACGGCCAGATTCTGGGTGCATTTTCA
    AGGTATCTCTAATAGGCTTTTCTGACAAACTGGATGTGGGATGTAAGACAAAAAGAGCAGTTGAGGATGG
    CTCCAAGGATTTTGGCCTGACAGAAGGACGGAGTTGACAGTATCTGAGATGGGGAAGGCAGAGAGTGAAG
    ATAGCTGTGGGGGAAGGTTGGGGCATTGGTGTTGGCCACAGGATTACAGGTGGAGATGTTGAACAAACCA
    TGGGGGCTGTTAAGATGGAGTTCAGGGGAGAGCTGAAGGCTTGAGATCCCTTTGGGATGTGGCAGAGTAT
    AGGTGATATTTAAGGACATGAACGTGAGTGGCTGGGGAAGCCAGTGAAGATGGAGAAGAGGAGAGACTCA
    AGGGCCCAGCCCTCGGGCATCACACCATTAATAGCTCAGGGAGATCAGGAGGACACATCTGGGGCCCCAA
    GGTTTCCTCTCTGGGAATCCAAGCCCAGGGGTATGTCTCTTCCCTGTTTTCTGTAGCCCTCTGCTTAAAC
    CCATAGGCTGAGCGCTCACTTTGGGATTATTGTTTTTAGTGGAGAAGGGGGGTAATGGGAGTGTGACTGT
    TTACAGTTAACAGATGTTAATGTCAATTTGACTGATGCTTTAAAATAGTATTGCCTGGGTTTTCTCAGGC
    AGTGAGAATCTTTGGATTCTTTCTCCTCCTCTACCTTCTCCAATCTCTGCAGGAGATAAGTCCCCCTTCA
    TATCATATCAGTTATACTGGCATTGCAATCACTTCATTTTTTTCTAGCTTTTAGAAAATCCTGTCTCCCA
    TTTTCCAGTTCCTCCCTGGCCCTCATTTGTCCACTCTAGCCTCTGGTACCAAAACTCTATTGCCATTTAC
    AGCTTTTTCTGACCACAGAAATCTTGGATAGGATCTCAGATGTCTTCATTGGCTAACTCAGACCTCTCAA
    TCTTAGAGACTCCAACCCTCCCTCATGTTTGTGTTCACCTGTTCACCTGTTACAGTTTATCTGTGAAATG
    TATATTTATATATCTTTCCATCTTGTTTTCTAGGCTGTGTAGCAAGTTCTTCTTGGACAAGGCCTAGCTA
    CAATTCTCTAAGCCTTACTTTTTGGTACATAGGGCTATGTATTTAGTACATAGGGCTATGCCCATTTGAG
    GGCAACAGAATGAAGCTGTATAAGTTTTCCATTCTCTTCCTGATTGACTTTCCCTTACTAAATCCTGGGC
    AGGTCTTGGAAAGAGAGCACACCTGTCGCAGGCCCTGGGCTGTGGTTAATGTATGCATCTGCTTGGCAGG
    GCCTGCCAAGCCTAACAGAGAGGACGTCTCCTCGTCCCAGGAAGAAAGTCTGCAGCTGAACAGCATCCCG
    CCCACGCCCACCCTCACCTCCACAGCTGTGAAGAGCAGGCAGCCCCTGTGGGGGTTGAAAGAGATGGAGG
    AAGAAGATGGCTCAGAGTTGGATTTTGCCCAGGGGTGAGGCCATGGAGGGAATCTGGGTGTCTGAGTTCC
    CAGTTTGGTGTCTGGCTGCCTCCATCTTGCTGTTTCTGTTTCATGGTTCCCTGCTGTAGCACTCCCACAT
    TGTTGGGTTCTTCTCAAACCCTTCTCCATTGTTTAGCTTTAAAATGCATCTCTCTGAGCTTTTCTCCCCA
    TTAACACCAGCTGCCTTACATCCTCTTCTTACCCTTGATTTTTATCCCATCATTGATCCTGCTTCATTCC
    CAGCAGTCAGCCCGTCGCAGGCACCGAGAGGTCAAGGTTCTTGGGTCCACAATATTTCCAGACTCCACAC
    AACCCTTCAGGTCCTGGCCTGGGCAACCAGCTGATGCGGTGAGCTTTTCTCATCATCTCCTGTCTTCACT
    TCAGATCTGTCGCCCACTGTGAGGGGATTCCTGTCTCACCCATTGCCTCTCTGTGGGTGCTTTTTGGACT
    TGTTTTAGCCACAGAGCACTTCCTGTCAGCACAATCGGGAAACTACTATGCTGTACTTACTGCTCCCCAC
    TTCCCCCACCCCGGGTGTCTGAGAAACTCTCAGGGGTCCTCAAAGAACAGTTTGAAAAGCCAGTCTCTTT
    GCCCCTACATCAGACTCAGCCTTATGGAAGAGGACGAGGAAGAAGAGTTAGAAATCCAGGATGAGTCAAA
    TGAAGAACGGCAGGATACAGACATGGTGAGTAGACCACCCTGCCCTTCTCTCTCAAGAACTAGGGGCTGA
    TGTGCCAAAGAGAAGCCAGGGTGTTTCCCCTCTCTCCGCTCGGTGATGCCTGGTCTGGAAAAGCTGAATG
    AATCTAGAGTAAAGGAGGACAGGTTATATGTTGGTTTGGACTTTGCTTGGACTCTACTTTTATTAAAGAA
    TAAGGAAAAGAAATTACAAATACCAAAGAAGGTTGGAATAGCACTCTCGCTCCCAATCCCTAAATAAATA
    TTGTTGCCTCCGAACCCCCTAGGAATTGGAAGGAGTCCTAATGCTGGAGGTGGGAGGTCATTCAGTCCAA
    GTGGCAGCCCCTTCAGTGTCTCTCCCCACAAAACATGACCCCCTATCCTCCATCTCCCTACTAGAGGGCT
    TCCCCTCCCTGTCCCTTCCCTCACTCGCTTTCTAACCTAGCCTGTTTCCCCAGGAGGAACTGCTGCTGGT
    GTAGTGGGAAAAGCCTGGCATTTGGAGTGAGGTGACATGAGTGCTAGTCCTCAACTCCATGTCAAGTTAG
    CGCTTTTACCCCTCCTCAGCCTCCATTTTCTCATCTTTATTTTTTAATTATTTTTATTTTTTGAGGTAGA
    GTCTCTCTCTGTCACCTAGGCTGGAGTGCAGTGGCATAGTCTCGGCTCACTGCAACCTCTGCCTCCTGGG
    CTCAAGCAATTCTCCTGCCTCAGACTCCCAAGTAGCTGGGATTACAGGCACGCGCCATCATGCCCGGCTA
    ATTTTTTTTGTATTTTTAGTAGAGACGGGGTTTTGCCATGTTGGCCAGGCTAATCTCGAACACCTGACCT
    CAAGTGGCTCCACCTGGCTCAGCCTCCCTAAGTGCTGGGATTACAGGTGTGAGCCACTGCGTGGGGCCCA
    TTTTCTCATCTTTAAAACAGAGCTGTTAGCCTGTAGTTCCAGCAACTCGGGAGGTGGAGACAGGAGGATC
    AATAGTCCAGGAGTTGGAGTCCAGCCTGGGTAACATAGTAAACACGTGTCTCTTAAAAAAAAATCAACAA
    TTCAAAAATAAATAAAATGGACCTTTTACATAAAATATAAGGTAAAACCACCTTATAAATGGAAAAGGGC
    TATGTCTGAGTTCAGGCTACTGTAACAGAATTCCATAGACTGGTGTCTGACAAACAGCAGGTATGTATTT
    CTCACAGTTCTGGAGGCTGAAGTCCAAGGGTTGGGTTCTGGTGAGGGCCTTCTTCCAGCCTGTAGACTGC
    TGGCTTCTAGTTGTATCCTCACCTGGAGGAGAAACAGCAAGCTAGCCCTCTGGCCTGGGCTTAGAAGGGC
    ATTCATCCATCCATGAGGGCTGCACCCCTATGACCCAATCACCTCCCAAAGGCCCCACTTCCAAACAGCA
    TCACACTGGGGGAAGGATTTCAATATGTGGGTTTAGGGGGACACAGATATTCAGTCCATTACAGGCTGCG
    TATCTTCTGCTGTTATTCTTCTGTTCTCTTCTCTGCAGGGAAGGAAAAAGAACAGAATAGAACAGAACAG
    GGGAGTCTTGTGCTCAGAAGGAGAAACCAAACAATGATACCTTCAAACCAGAGACTAGAGAGCAAGGCCT
    CTGTTAAAATATTATTTCCTAACATCTCAGAAAAAATCCGTTTTCTGGTTTCCAAAACAAAGTCATTTAA
    GGGGCTCAGTCAGCAGGCCAAGAAGCGCTCTGCAGTCAGGCCTGATCCTGGTGCCCACACTGACTTCCCT
    CCCAGGAGGTGCCATAGGGGCCTGCTGTGCCCATATTTGTTCTTATGAGGCTGGGAAAGGAACTGGTTTT
    TTTATTTGCGAAGTGACAGGAGTGTGTAAAGGCCTTTTTGACTTTTAACCTCATTCTCTCTAGCAAGCAA
    GTAGCTACTCTTCCACCAGTGAGCGCGGGCTGGACCTCTTAGATTCTACAGAGCTGGATATTGAGGTGAG
    TGTCCCCAGAGCAGGAGTTATGTATCCTTCGGAAATGCTGGCAGGCAACCCGTGCACTCATCAAATTGAC
    AGGCCATAGCCTGGGGGTGGCCCTCTGGGCTGTCTCTGGGGCTTTGAGGGTAACCCAGGGGAGGATGGTC
    TGCCCATTGGCCCGTTGCTGTGTCCTGTGTATTCCTTTCATCTTTCTCATTATAGGATTTCTGACAGGAC
    TCTGGGCCCCTCCCCAGCTCCACTCCCTACCTCAAGAATGTGACCATTTGGAAAAGGCAAAGAGAAAAGG
    AGCAAAATGAAGCATTCCCCCAGGCTTCAGCCCTGGGCTCTGAGGGGAAAGAGTTGGGCATTGTTTTTCT
    AACCTAACCTTTCCCTCTGGGGTAGAGAAGCCGAGAGACCCTGTCCTCCCTTATGCACTGTGGCCCAGTC
    CCCTTGCCTTTTTCCTGTTCTGTTTGGAGTGGAGAAGGGCAGCACCTCTGTGTTTAATGGAAATAGCCCA
    TAGTCTCCTGGATTTTTGGAACATCTTTCTCAGCCTATTTTGTGTCCTAATGATTCGCTCAATAAACATG
    TTTGAATCCACACGT
  • Protein Sequence : Show Sequence
    >gi|57863310|ref|NP_036579.2| cohesin subunit SA-3 [Homo sapiens]
    MSSPLQRAVGDTKRALSASSSSSASLPFDDRDSNHTSEGNGDSLLADEDTDFEDSLNRNVKKRAAKRPPK
    TTPVAKHPKKGSRVVHRHSRKQSEPPANDLFNAVKAAKSDMQSLVDEWLDSYKQDQDAGFLELVNFFIQS
    CGCKGIVTPEMFKKMSNSEIIQHLTEQFNEDSGDYPLIAPGPSWKKFQGSFCEFVRTLVCQCQYSLLYDG
    FPMDDLISLLTGLSDSQVRAFRHTSTLAAMKLMTSLVKVALQLSVHQDNNQRQYEAERNKGPGQRAPERL
    ESLLEKRKELQEHQEEIEGMMNALFRGVFVHRYRDVLPEIRAICIEEIGCWMQSYSTSFLTDSYLKYIGW
    TLHDKHREVRLKCVKALKGLYGNRDLTTRLELFTSRFKDRMVSMVMDREYDVAVEAVRLLILILKNMEGV
    LTDADCESVYPVVYASHRGLASAAGEFLYWKLFYPECEIRMMGGREQRQSPGAQRTFFQLLLSFFVESEL
    HDHAAYLVDSLWDCAGARLKDWEGLTSLLLEKDQNLGDVQESTLIEILVSSARQASEGHPPVGRVTGRKG
    LTSKERKTQADDRVKLTEHLIPLLPQLLAKFSADAEKVTPLLQLLSCFDLHIYCTGRLEKHLELFLQQLQ
    EVVVKHAEPAVLEAGAHALYLLCNPEFTFFSRADFARSQLVDLLTDRFQQELEELLQSSFLDEDEVYNLA
    ATLKRLSAFYNTHDLTRWELYEPCCQLLQKAVDTGEVPHQVILPALTLVYFSILWTLTHISKSDASQKQL
    SSLRDRMVAFCELCQSCLSDVDTEIQEQAFVLLSDLLLIFSPQMIVGGRDFLRPLVFFPEATLQSELASF
    LMDHVFIQPGDLGSGDSQEDHLQIERLHQRRRLLAGFCKLLLYGVLEMDAASDVFKHYNKFYNDYGDIIK
    ETLTRARQIDRSHCSRILLLSLKQLYTELLQEHGPQGLNELPAFIEMRDLARRFALSFGPQQLQNRDLVV
    MLHKEGIQFSLSELPPAGSSNQPPNLAFLELLSEFSPRLFHQDKQLLLSYLEKCLQHVSQAPGHPWGPVT
    TYCHSLSPVENTAETSPQVLPSSKRRRVEGPAKPNREDVSSSQEESLQLNSIPPTPTLTSTAVKSRQPLW
    GLKEMEEEDGSELDFAQGQPVAGTERSRFLGPQYFQTPHNPSGPGLGNQLMRLSLMEEDEEEELEIQDES
    NEERQDTDMQASSYSSTSERGLDLLDSTELDIEDF
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
38. TBX21
  • Gene Name : TBX21
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 30009
  • NCBI Protein GI : 7019549
  • Genbank Accession : AC015674
  • Protein Accession : NP_037483
  • Taxonomy ID : 9606
  • Chromosome No : 17
  • Gene Starting Position : 41179682
  • Gene Ending Position : 41192555
  • Gene Strand (Orientation) : +
  • Protein Name : T-box 21
  • Protein pI : 5.92
  • Protein Weight : 55747.12
  • Protein Length : 535
  • Protein Note : Also known as TBET; T-PET; T-bet; TBLYM
  • DNA Sequence : Show Sequence
    >gi|157713538:41179682-41192555 Homo sapiens chromosome 17, alternate assembly HuRef, whole genome shotgun sequence
    GCGGCCCGCTGGAGAGGAAGCCCGAGAGCTGCCGCGCGCCTGCCGGACGAGGGCGTAGAAGCCAGGCGTC
    AGAGCCCGGGCTCCGGTGGGGTCCCCCACCCGGCCCTCGGGTCCCCCGCCCCCTGCTCCCTGCCCATCCC
    AGCCCACGCGACCCTCTCGCGCGCGGAGGGGCGGGTCCTCGACGGCTACGGGAAGGTGCCAGCCCGCCCC
    GGATGGGCATCGTGGAGCCGGGTTGCGGAGACATGCTGACGGGCACCGAGCCGATGCCGGGGAGCGACGA
    GGGCCGGGCGCCTGGCGCCGACCCGCAGCACCGCTACTTCTACCCGGAGCCGGGCGCGCAGGACGCGGAC
    GAGCGTCGCGGGGGCGGCAGCCTGGGGTCTCCCTACCCGGGGGGCGCCTTGGTGCCCGCCCCGCCGAGCC
    GCTTCCTTGGAGCCTACGCCTACCCGCCGCGACCCCAGGCGGCCGGCTTCCCCGGCGCGGGCGAGTCCTT
    CCCGCCGCCCGCGGACGCCGAGGGCTACCAGCCGGGCGAGGGCTACGCCGCCCCGGACCCGCGCGCCGGG
    CTCTACCCGGGGCCGCGTGAGGACTACGCGCTACCCGCGGGACTGGAGGTGTCGGGGAAACTGAGGGTCG
    CGCTCAACAACCACCTGTTGTGGTCCAAGTTTAATCAGCACCAGACAGAGATGATCATCACCAAGCAGGG
    ACGGTGAGTGCGGCGCGCCGGCCCTTGGGGCCTCTGTGCCCGCGCCGGAACAAGAACGTCTCGTCTGTTT
    TTCTGGCTCGACAATGCTTCTGACTCCGTGTCCCTCACTGCTTTGGCTTCAGCGTAGGGAGACAGGGGAA
    TGGGGTTGTTAGGAGGACAGGGAAAGCTCCGGAGGGGCGTCTGTGCCCAGGCTGTTGCACCAACAGCCAG
    AGGACTCACAAGGGAGACGGGTGAGTGCGGGACAGTGAGAAGTCACCTTGATTTAGGGGAAGGGTGACTG
    TGGCTTCACCTAGAATTGGTGTGCGCCCCTGCCCCACTCTCTACTGTAGAGGAGTCGCAGCGGGCAGTGA
    AAGCCTGTGCTCTGGGCGGACAGGACGCCTGGGCCTCCTGTGTGGGGAAACTGGAGGGGAAGGGAGCCCC
    TTATCTCCGGGCCCCCTGCGCCCACCTCCCCCGGCTCCTTTGCTGCTGGTGTGCTCAGGTCAGCTTTAAT
    GGTGGTAGTGGTGGTGGTAGCGGTGGTGGTGGTGTGTGTGTACGGGGGGAGATTGGGATTTGGTGACATG
    GAGAAGCAGTCGCCAAGTTTCCTTTCCGGTCTTACTTTGAGATCATATGTCTGGTGTGTGTGTGTGTGTG
    TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTGTGTGTGGGTGTGTGTGTATGGGGGCTAGTGCA
    GTAAAGCTTGTAGGGGGCACAGATCCCTTCCAGGCACAAATGCCCACGGGCTGGGCAGATGAAGCTCACC
    CAGGGGTCCAGCCTGGTAGCCAGCCCCACACTGCACCCTTTGAGGCTGGTCCAGTGAAAACCTTCCCCAC
    ACTCCTGTCCAGAAATTCACCGGTTCAGCCTGGAGAAGTGGGGAAGGGGTGTCCCATGGCTTCATGGCTC
    AGGGTTCCTGAGCCCCGTGCGTGATGGGGAGAGTTTGGGGCTGAGGGTGCTGCTTCCGGATAGAGCCTCC
    TGCGCAAGGAAAGAAACAGAAACCTGTGACTTGTGTGGTATTTGTTTAGTAAGCAACCCCCGGAGTGGAC
    TGTGTCTGATGTGGGCTGTGCGCACCCACCCTTCCCAGTGCGGCCCATGTGAGCAGGGGACCAGCGAGGA
    CCAGTGTGGAAGGGCTGTTGTCATTGGTGGACCTGGGATGCTGGGTCCCAGGTCCGAGAGGTGTGGATAC
    CAAACGTGGGGCTTGGGGTGGAGGGGAGAGGGAGAAGGCCATGTTGGACCCCAGAGGTTGGTATTCGATC
    TGGGCATTGCTGGAACATTCTTCCCTCCAGATGATTTTTGTGGGGTAGCCTGGGACTGGGGAACCTGTTG
    CCAGCCACAACTGGCTTCAAGTTACTGAGCTGTTCCACTTTCCCTGGGATGAACCCAGGAAAGTTGGCGT
    GTTCTTTGATGGCTCAGCCTTCATCTCTTAAGCCTTCTGATAATTTCTCTCTGCTCCCCCCAACCCTAGT
    CACCCTTTGGGTGTACCCAGACACAGCCCAAGCTTCTGGTCCCAATGTGTCTGAATTGGAAGGGACCCAG
    AAGATCCTGTCCCTCTCACTGCACAGACAGGGAAACAGGCCCAGAGATAGCAAGAGACTCTTCCAAGGTC
    ACACAACACACTCATTGCAGGCAGAGCTGGCACTACAGCCCAGATAGTATTTGCTTGGGTTTCTTTCTCT
    GGCCCCCTACGGCCACCAGTAAAAACAGCCACCCACGCTCTCTGAAGGCCCTGATGCATTCAGAGTGCTT
    TCACTTCCATTATTTCACTGTGCGTTAGGTAGGCCAAGCAGTCTCCCCATTTTACAGTTGAGGTCACTGA
    GTTGCAGGGTCAGCTAGGGGCTTGCACTCCATCACACAGCAGACCAGAAGTGGGGCCTCTTAACCTTCCA
    CTCTGTTGTGTGGTCAGGAGGACTTCCCACCCTGGGCCCTTGCTGGGGTCTCCCCACAGCCTCTGCCAGG
    CCTCTGCCTCCTCCTGCCAGTCTTGAGGGATGGGGTGGATGGAGCATCAGCCAGCACTCTAGGGAGTTGG
    CCAGTGCTAGGGGGCTGCCTCTCTGCCTGTAGAGCCAGCTTCAGGGAGGCTGTAGAGCACTTCAGGGAGG
    CACTTGAGGGGATGATTCTCGAAGTGTGTATCACCATCTCTACTCCCATGGCCTCCTTTCCTTGGTGTTC
    CTGCTGAGTAATTCTCACTTGACAAGTTTTTTTGTCCCCCTTTTAGATACATACACTTACATTTTTTTAT
    TATGAAAAATTCCACTACATACAAACATGGACAGAAGAATGTAACAGACTTCCATCCCCAGCTTCCACAG
    TTGTCAATATGGGTCCAATCTTGTTTCTGCATCCTCACTTCGCTTCTTCTGTCTGCAACTATATTAGTAA
    TATAGTAATTACTAAGTACATTCTAGATATTATTTTATCTCTGCACCTCTAAAAATGACTTTTTAAAATG
    AATGCATTACCATTAGCAAACCTGAAAAGAATTTCCTAAATATCACCAAGCTTCTCTGATTGCTTCATAA
    ATGTGTGTGTGTGTGTGTTTTCCAATCCTCACACTTTTTGCTCCATTCTGAGGGGCTCCATACGCGGGGT
    CCCTGTTGAAGGAGGCAGTGGCTCTTCACTGTTTCTTTTGGTGCTTTTTCTTGGATCAGGCTTCATAGAC
    CCCACGCTGCCATGGAGGGGACAGAGGACACACAACCCCAATTCAGTCCCACTAGCCCCAGGTTACCTCC
    ATACCTTCTCAGCTTGGGCTCCTGAGATCTGGGAAATCAGTGGCCATTTCCTCTCAAAACAGGCTTTTGG
    GCTTATAGCACAGCTTCCTTTCCGTCTTCCTGCTTTTGTGGGCTGGCCTATCCCTCCGTGGGTTCCAGTG
    ACCCACCAGTCACACCACATCACCCATGACCTTCTTCCTTTTTGAAAGGTTCTCCCACCTCAGGCCCACC
    TACAGCCTACAGCCCCACGGCTTTTTGCCTTGAGCTGACTCACGCCGTCCCGGATTCTTCCTCTCCCACC
    CACCCTGGATCTATCCCTCGGTATTGACCAGCGCCAGTGGCATGCTCTTCCCAACGGGCAGAAATGGGGC
    AGGGCACAGGGGGTGATTCCTGGGTCTGGAGATTGATCAAGGCAGCTCAGATGGGGCTGAGAGAGGGGAA
    CTGAGAACAAAACGGTGGATTATCCCAGTAGGAGACTTTGGTGTCTACGTTTGTGTCTGGAGATTTGGGG
    CAGCCTGATAATGCCCCACACAGGCTTGTGCGAGGGTTTCTGTGTGGCAGTGTGTGTGTGTTGCGAATGT
    GTTGGCGAGTATATAATAGTTGATGGTAGACATTCTCAGTAGAGGAAGAGAGAGGCCTGTGTCTCTGTGG
    GTGTGTGGGTGTGTATTAATTGCTGGCAATCCCCACTTTCTGGGTGTGATTGGTTGATTATTATAATTAA
    GATAACTTTGGGTAGCTGTGTTGTGTTAAGCTCAATCTGCATTTGGATTTGTGCATGTGAAGACAGAGGT
    TGGTAAAACAACAATTTCAGTCCCACACTATCCTTGCTATCCTCACAGTCATTACAATTTTTTTTTGTCT
    CCACTTTCCCAACTCCAAGAACTTTAATTTTTTTATTTGTAATATTTTTCATGGGCCAGGAAGTGGACTT
    GAACCTCCCACATAGATAACTTTAGTCGATAACTTTCTACATCTTTTTTTTTTTTTGAGATGGAGTCTCA
    CTCTGTCCCCCAGGCTGGAGTGCAGTGGCTCAATCTTGGCTCACTGCAACCTGCACCTCCCAGGTTCAAG
    TGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCAGAAGCCACCAAGCCCAGCTAATTTAT
    GTATTTTTAGTAGAGACGGGGTTTCGCCATGTTGGCCAGGCTGGTCCCAAACACCTGACTTCAAGTGATC
    CCCCGCCTCGGCCTCTCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCTGGCCCAACTTTTCAGAT
    CTTAAAAAATTTTTTTTAATTTATTTTTTATGGAGACAGAATATTACTGTGTTGCCCAGGCGGTCTCAAA
    TCCCTGATCTCAAGCAATCTCCCGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCATTGTGC
    CCAGCTAAAAACATTTTTTTTAGGCTTAAGCATGCTTAACTGGTTTCATTTGTCTTGGGGCAAGTGAAAA
    CAACTTTAATTAAAAATTTTTTTTAAATGAGACATGGTCTTGCTCTGTCACCCACGCTGGAGTGCAGTGG
    TGCGATTATGGCTCACTGCAGCCTCAACCTGCTGGGCTCAAGCAATCCTCCCATCCCAGCCTCCTGAGAA
    ACTGGGACTATAGGCGCACATCACCATGTCCGGCTAATTGTTTCTATTTTTTGTAGAGATGGGGTCTCAC
    TATGTTGCCCAGGCTGCAGCTTTGCTTTTTGTCTGGCTGTTTGCCAGTGGCTGTCTGAGTCTATTAAGTG
    GATCTGAGGTTGGTAAGACGAGGAGTTCCCTTGATGCCCAAGCACATTTTGTACTTTGGTAGGTACGACA
    CATTGCCAGAATACCCTCCCAAAAGGTGCTACCAATTTACGCCCACACCAATAGTCTATGAGAGGACCCA
    TTTTCTCACAGCCTCATCAGCAGTAGATATTATCACATTTTTTTTTTTTTTGAGACAAAGTCTCGTTCTG
    TTGCCCAGGCTGAAGTGCAGTGGCATGATCTCTGCTCACCGCAACCTCCGCCTCCCAGGTTCAAGCGATT
    CTCCCACCTCAGCCTCCCAAGTAGCTGGGATTACCGGCATGCACCACCACGCCTGGCTAATTTTTGTATT
    TTTAATAGAGATGGGGTTTCACCATGTTACCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCCACCT
    GCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCACGCCGGGCCAATATTATCAATTTTTA
    AACATTGACCAATATGACTGAGAAGAACTATCTATCTCCTTGCTATTTTAATTTGCACTTAATTACAGTG
    AAGCAGAGCCTGTGCCAGGAGCTCCGAATCTGGGAGAGGTATGACGACCCTGTGAACCATCCCAAGGCTA
    CTTAGTTCTTAGGGCCAGCAGAGGAATTTGGGGTCTTGGACCCTGTCTTATGAGGGTGGGATGGTAAGTG
    GCCTTTAGGGACGCTCAATTTGACACCAGACCCATCACCACTCTCGGTTCCTTCCCAGCAAAGTGTCATG
    TAAATCAGGGGCTATTTTGGGATTCCAGCTGGTGGTGATTCTAGACTTTTAGGGAGAAACCGAGATCTCC
    TTTCTCTTCTCCCTTTTCCCCAGCCTCTTCCTGTGTCTCCATTTCCCTCTACTAGTGATAAAAACAGGAT
    GAGCTGGGCATGGTGGTGAGCACCTATAACCCCGCTCGGGTGACTCGGGAGGCCAAGGCAGGAGGATTGC
    CTGTGCCCAGGCATTTGAGCAGAGCCTGTGCAGCATAGCAGACTCCATCTCTTAAAAAAAAATCAGTATG
    GCCTGGGCGCGGTGGCTCACCCCTGTAATCCCAGCACTTTGGGAGGTCGAGGCCAGTGGATCACCTGAGG
    TCAGGAGTTCGAGACCAGCTTGATTAACATGGTGAAACCCCGTCTCTACTAAAAGTACAAAAATTAGCCA
    GGTGTGGTGGCGGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATAATGGCGTGAACCT
    TGGAGGCGGAGCTTGCAGTGAGCCAAGATCACACCACTGCACTACAGCCTGGGCAACAGAGCGAGACTCC
    GTCTCAAAAAAAAAAAAAAAATTAGCCAGGCGCAGTGTTGGGTGCCTGTAGTCAAGGAGAATCTCTTGAA
    CCTGGGAGGCAGAGGTTGCAGTGAGCCGAGATCACACCACTGCACTCCAGCCTGGGGGACAGAGCGAGAC
    TCTGTCTAAAAAAAAAGAAAGAAAAAAATGAATATGAAGAAATGGGGACCACAGAGCACAGCATGCACTC
    TGCAGTCAGAAGACCTCACCTCTGCTCTCCCACCGAGGACCGTCACCAAACCTCTCGGAGCGTCAGTTTC
    TTCAAATATAAAATTGGAAATAACATTTTATTTTTTATTTTTTCTTTGAGATGGAGTCTCACACTGTTGC
    CCAGGCTGGAGTGCAGTGGCGCCATCTCGGGTCACCGCAACCTTCGCCTCCCAGGTTCAAGCGATTCTCC
    TTGCCTCAGCCTCCAAAGTAGCTGGGACTACAGGTGCATGCCACCACACCAGGCTAATTTTCTGTATTTT
    TAGTAGATATGGGGTTTCACTATGTTGGCCAGGCTGGTCTCGAACTCCTGAGCTCAGGAAATCCACCCTC
    CTCGGCCTCACAAAGTGCTGGGATTACGAGTGTGAGCCACTGTGCTGGGCCATCTTTCGAACTTTTTAAC
    AGCTTTCAGAGGAAAGATGGACAGGAGTTAGACTCTCCATTTACATGTGAAGAAACTGAGATTTGGGCTG
    GGTGCAGTGGCCCATGTCTATAATCCCAGCACTTTGGAAACCTGAGGCGGGTAGACCACTTGAGGTCAGG
    AGTTCGAGACCAGCTTGGCCAACGTGAAACTCCATCTCTACTAAAAATACAAAAATTAGCCGGGTATGGT
    GGCACATGCCTGTAGTCCCAGCTCTTGGGAGGCTGAGGCAGGAGAATCACTTGAACCTGGAAGGTGGAGG
    TTAGAGTGAGCTGAGATCACGCCACTGCACTCCAGCCTGCATGACAGAGCGAAACTCCATTTCAAAAAGA
    AACTGAGATTTGGCTTGAGAATTAACTTATCCAGGGTCATAGGGTAGTAATCTATTTTTCTCAACTCAGG
    AAAGTCAGGGGAAGACAGGCGTGTGACAGCACCCTTCTAGGACACCTTCCCAAGGTTGTTAGGTAAGTTG
    TCCTGGGGACCTCTGGGGATTAGAAGTCCCTCAAAAACAGTTCCTAGGCCCCTGGAACCCTCCCTCCTCC
    AGGTTTCCTGACTCAGATAGGTCCCTTTTTCCTCATCCTTCAGGGCTTCTCATGAGAGGAGGGGGAAGTG
    TGTGGAAATGAAAAATAATTCTGATGAGGATTAGCTTCTGCCATTCTGAAAACTCTGCTCTTCCTGCTTG
    TGTTAGAAGAGAAAGGCTAAGCCATTGGAGCTGGTGATGGAATTGGTGCTGGTGGAGGTGGTGGTTGTAG
    TGACGGTACTACTGGTGGTGGTTGTGATGGTGAAGGTGGTGGCTGTAGATGGTGGTGCCCGTGCTGGTGC
    TGGTGGAGATCGTGGTTATCGTGATGGTGGAGGGGGCAGTTGTAGTGACGGTGGTTGTGATGGTGGTGAT
    GGGTGGTTACAGTGCTACTGGTGGTGATGGTGCTGGTTGTAGTGGTGGTGGTGACGGGGCTAGTGAAGGT
    GTTAGTGGTGGAGATGGTGATGGTAGAAGAGTGCTTCTTTTCTGAATCTGTTACTCTGGCACAACAGCGG
    AATCATACAGCATCCCACCCCCTAACTTCCTCTACTTTTCCTGGAAACAAGGACTTTGAACATGAGCCCC
    GTGTTCAATAGAGCCTTCTTTCAGGTGGATGGGAAGCTCCCTGTTCAACTAGGATGTGTGCAACTTCTCT
    AAAGGCTGAGGGCAGACTATACCGCAGGTGGCAGACCCAGGAGCATGGGAGAGGGAGGGCCTGTGAGTGG
    GTGTCATGGGTCTGGGAGTGTGTTAGCACTGTGAGAGCCACGGCAGAGAGGGTGGCTGAGGAACTGAGGC
    GTGAGGACCTTCCAGGAACTGCCTGTGGAGAGCTGGTGGTGCCCCCTCCTAGGAAGCAGCTAAGGTTAGA
    GACAGGGGTGTGCAGGTAGATGTCGAGCAACTGACCCTCTGAAAGAACATTTTAAAAAACTCTGATTTGT
    AGTGTTTGCTGATCCCTGTGGTGTAAATACTCCTGCTATGGATGATTTCAAGCGACTCATGTGAGTCATT
    GAACACAGAGCCGGGAAGAGACTCACAGTGCACTGTGATTTAGTACCTCCTCCATGCAGCTGCGATAGAC
    GGAAGTAACCTCGACAGTGCAGACAATAGTAAAACGTAAAATAACTGGGCAGTGATGATTTTTTAGTTTT
    TACTCCTTTTTTTTTTTTTTGAGATGGAGACTTGCTCTTGTCATCCAGGCTGGAGGGCAGTGGCGCGATC
    TCAGCTCACTGTAACCTCTGCCTCCTGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTAGGA
    TTACAGGCGCCCACGACCACACCCAGCTATTTTTGTTGTTGTTGTTGTACTTTTTAGTAGAGACGGGGTT
    TCGCCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCGCCCGCCTTGGCCTCTCAAAGT
    GCTGGGATTACAGGCATGAGCCACTGTGCCGGGCCTACTTTGACTGTTAATATGTCTTGAATTGCAAGTT
    TATATAATTTAATTTTTAATTATGGCTGTGTTTAACCACTGGCTGGCAAACTCCCTAAACACCTTCCAGC
    TGGTTCTTGTGAGTGGGAGGAAGCCGGCTACAGCACACCACTGATGCCTGGGCACTGTTGCAGGGGGGAC
    TGGCTGTCAAGCTGGAGCTGATGGGTGCTGGGGGCTTTCTCTCTTCTCTCCAGGCGGATGTTCCCATTCC
    TGTCATTTACTGTGGCCGGGCTGGAGCCCACCAGCCACTACAGGATGTTTGTGGACGTGGTCTTGGTGGA
    CCAGCACCACTGGCGGTACCAGAGCGGCAAGTGGGTGCAGTGTGGAAAGGCCGAGGGCAGCATGCCAGGT
    GCGCGCGCCCCTGGGAGCGGTGGGCTCTGTTTCGCTGGGACTGGGCGCCCCCTGGTGGGCCCACCAAGCC
    CCTACCCCTAATTCCTAGACCTTTAACCCCCTCCCACTCCATCCCACGCCATTGCATCCCTCCTGTTTCT
    GGCTTCCTGCTTTGCTCTAGCCTGTCCTCTGCTGAGTCCTCTGCCCTTCCCTGCCTGGTCCTCCCCCTGT
    GTCCTTCCTTACGTCCCTCTCGGGACAGGCAAAGCCCTACATCACCAGGGTTCTGTCCCGGGGGCTGCAT
    GTCAAAGAGGTGAACTGTCCACAGGAAACCGCCTGTACGTCCACCCGGACTCCCCCAACACAGGAGCGCA
    CTGGATGCGCCAGGAAGTTTCATTTGGGAAACTAAAGCTCACAAACAACAAGGGGGCGTCCAACAATGTG
    ACCCAGGTAGGACCTGCTCTTCAAAAGGTAGCCTCGCCCTGCTCCCCACCCTGGGTCTGAGACCTCCAAG
    GCCACAAGGGTCCTGCGGGGCCAGTTTGGTTCATTTTTTCTTCCTTCCTACAGGAAGGAAGGTTCGTTTT
    TCTTCTGTCCTAAACGAAGGGTCATTTTTCCTCCTTCCTAAACTCTGGCTGTTTCTCACCCACCTTGGGA
    GGAAGATGAGATGGGATGAAGCTGAATCTTGGACGGGGGTCATATTCAGGCCACACAGGCCAACCCAGCC
    TCAGGGTTTCTTCACCAAATCATGGGTTGCCATTGCTCCGGCACCATGAAAGGCACAGAGTTGGCCCTGT
    GGTCCCAGGGAATAGATGCCGAGTTTCTCTAGGTTGAACAATCCTGTTAAAAGTGCCCTTGCTGGCTGGG
    TGTGGTGGCTCACACCCATAATCCCAGCACTTTGGGAGGCCAAGGCGGGTGGATCACAAGGTCAGGAGTT
    CAAGACCAGCCTGGCCAAGATGATGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAAGCATGGTG
    GCGGGTGCCTGTAATCCCAGCTACTCAAGAGGCGGAGGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGG
    TTACAGTGAGCTGAGATCATGCCATTGCACTCCAGCCTGGGTGACAGAGTGAGACTCCGTCTCAAAAAAA
    AAAAAAAAAGAAAAAAGAAAAAAGAAAAAAAAGTGCCCTTGCCCTAAAGCCCTGTTTGTGCTGATACCTT
    GGTCTCAATTGACATCTGGAGGTAGAAGAGGGGGCAAGAGGCTAGAATTGGTGCCCCAAAGAGTCCGGAC
    ACTGGAGTTGGAAAGCTTGGGGGAAGTTACATGGTGGCAGAGCAGGGGAATGGACAGGATGACCTCAAGG
    TGCTTGCTAGCCTCACGTGGGGCCAGCTGTTGGTGGGGGTGATGGGATCCTCGAAATCCTTCCTCCCCAC
    CCCTACAACCGTCTTGCTCTGTCTACAGATGATTGTGCTCCCAGTCCCTCCATAAGTACCAGCCCCGGCT
    GCATATCGTTGAGGTGAACGACGGAGAAGCCAGAGGCAGCCTGCAACGCTTCCAACACGCATATCTTTAC
    TTTCCAAGAAACCCAGTTCATTGCCGTGACTGCCTACCAGAATGCCGAGGTGAGGGCTGCCTGAGCCCCG
    GTGGGGAGGAGGGCAGAGTGGGGCCCACTGTCTTCCTTGGGAGGGATTTGGAAAGTTCCCGAGCCCCAGA
    CTCAGGACTCAGGTGACTCTATTTCCCTTCTCTCTAGATTACTCAGCTGAAAATTGATAATAACCCCTTT
    GCCAAAGGATTCCGGGAGAACTTTGAGTCGTAAGTGCCACTGGGTTCAACTCAGCTTTGGTCCCTCCTGA
    GACACATCCTCTCCCTGCCCCTGAAAACAGGAGGGTGGGGGACAGATGCTACAGGTGGGCAGGCCAGGGA
    AGGAGGGTCGGAGAAGGAATGTGTGAAACAGGTAGGCTCACAGGTGACTGGTTCTGCTTGTGACCCGTTT
    TCTTGCCTTCTATTTTTTTCTAGCATGTACACATCTGTTGACACCAGCATCCCCTCCCCGCCTGGACCCA
    ACTGTCAATTCCTTGGGGGAGATCACTACTCTCCTCTCCTACCCAACCAGTATCCTGTTCCCAGCCGCTT
    CTACCCCGACCTTCCTGGCCAGGCGAAGGATGTGGTTCCCCAGGCTTACTGGCTGGGGGCCCCCCGGGAC
    CACAGCTATGAGGCTGAGTTTCGAGCAGTCAGCATGAAGCCTGCATTCTTGCCCTCTGCCCCTGGGCCCA
    CCATGTCCTACTACCGAGGCCAGGAGGTCCTGGCACCTGGAGCTGGCTGGCCTGTGGCACCCCAGTACCC
    TCCCAAGATGGGCCCGGCCAGCTGGTTCCGCCCTATGCGGACTCTGCCCATGGAACCCGGCCCTGGAGGC
    TCAGAGGGACGGGGACCAGAGGACCAGGGTCCCCCCTTGGTGTGGACTGAGATTGCCCCCATCCGGCCGG
    AATCCAGTGATTCAGGACTGGGCGAAGGAGACTCTAAGAGGAGGCGCGTGTCCCCCTATCCTTCCAGTGG
    TGACAGCTCCTCCCCTGCTGGGGCCCCTTCTCCTTTTGATAAGGAAGCTGAAGGACAGTTTTATAACTAT
    TTTCCCAACTGAGCAGATGACATGATGAAAGGAACAGAAACAGTGTTATTAGGTTGGAGGACACCGACTA
    ATTTGGGAAACGGATGAAGGACTGAGAAGGCCCCCGCTCCCTCTGGCCCTTCTCTGTTTAGTAGTTGGTT
    GGGGAAGTGGGGCTCAAGAAGGATTTTGGGGTTCACCAGACGCTTCCTGGCCCACGATGAAACCTGAGAG
    GGGTGTCCCCTTGCCCCATCCTCTGCCCTAACTACAGTCGTTTACCTGGTGCTGCGTCTTGCTTTTGGTT
    TCCAGCTGGAGAAAAGAAGACAAGAAAGTCTTGGGCATGAAGGAGCTTTTTGCATCTAGTGGGTGGGAGG
    GGTCAGGTGTGGGACATGGGAGCAGGAGACTCCACTTTCTTCCTTTGTACAGTAACTTTCAACCTTTTCG
    TTGGCATGTGTGTTAATCCCTGATCCAAAAAGAACAAATACACGTATGTTATAACCATCAGCCCGCCAGG
    GTCAGGGAAAGGACTCACCTGACTTTGGACAGCTGGCCTGGGCTCCCCCTGCTCAAACACAGTGGGGATC
    AGAGAAAAGGGGCTGGAAAGGGGGGAATGGCCCACATCTCAAGAAGCAAGATATTGTTTGTGGTGGTTGT
    GTGTGGGTGTGTGTTTTTTCTTTTTCTTTCTTTTTATTTTTTTTGAATGGGGGAGGCTATTTATTGTACT
    GAGAGTGGTGTCTGGATATATTCCTTTTGTCTTCATCACTTTCTGAAAATAAACATAAAACTGT
  • Protein Sequence : Show Sequence
    >gi|7019549|ref|NP_037483.1| T-box transcription factor TBX21 [Homo sapiens]
    MGIVEPGCGDMLTGTEPMPGSDEGRAPGADPQHRYFYPEPGAQDADERRGGGSLGSPYPGGALVPAPPSR
    FLGAYAYPPRPQAAGFPGAGESFPPPADAEGYQPGEGYAAPDPRAGLYPGPREDYALPAGLEVSGKLRVA
    LNNHLLWSKFNQHQTEMIITKQGRRMFPFLSFTVAGLEPTSHYRMFVDVVLVDQHHWRYQSGKWVQCGKA
    EGSMPGNRLYVHPDSPNTGAHWMRQEVSFGKLKLTNNKGASNNVTQMIVLQSLHKYQPRLHIVEVNDGEP
    EAACNASNTHIFTFQETQFIAVTAYQNAEITQLKIDNNPFAKGFRENFESMYTSVDTSIPSPPGPNCQFL
    GGDHYSPLLPNQYPVPSRFYPDLPGQAKDVVPQAYWLGAPRDHSYEAEFRAVSMKPAFLPSAPGPTMSYY
    RGQEVLAPGAGWPVAPQYPPKMGPASWFRPMRTLPMEPGPGGSEGRGPEDQGPPLVWTEIAPIRPESSDS
    GLGEGDSKRRRVSPYPSSGDSSSPAGAPSPFDKEAEGQFYNYFPN
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
39. TLR1
  • Gene Name : TLR1
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 7096
  • NCBI Protein GI : 41350337
  • Genbank Accession : AC108044
  • Protein Accession : NP_003254
  • Taxonomy ID : 9606
  • Chromosome No : 4
  • Gene Starting Position : 38797875
  • Gene Ending Position : 38806411
  • Gene Strand (Orientation) : -
  • Protein Name : toll-like receptor 1
  • Protein pI : 7.07
  • Protein Weight : 86022.3
  • Protein Length : 786
  • Protein Note : Also known as TIL; CD281; rsc786; TIL. LPRS5
  • DNA Sequence : Show Sequence
    >gi|224589816:38797875-38806411 Homo sapiens chromosome 4, GRCh37.p5 Primary Assembly
    CTTATGTGTCAGAACTGTTTAAATCAAAGTTATATCATTTCATTAATTTTTAATACCACATATAAATGGT
    GAACTGCGACCCGAAGGTATATATTTTTACCTACATCATACACTCACAATTGTGTTTACATCTATGCTGA
    TGCAAAATAAAGTCATTGTTGGAACTTCCAAAAGCAGCAATATCAACAGGAGGAATATTTTTCACTTGAT
    GTGTAATCTATTTCTTTGCTTGCTCTGTCAGCTTAATATTAATGGCTGCCCTTAAGTTAGCCCAAAAAAG
    GCCACGTTTGCTCTTTTCCTTGGGCCATTCCAAATAAGTCCTCCTGGCCATGAGACTTTTGAGCTTGTGA
    TAACTGCTAGGAATGGAGTACTGCGGAATGGGTTCCAGCAAGATCAGGATTAAGCTATTAGATCCTTCAT
    GAAAGAGATTGTGATGGGCAAAGTAGAGTTCATAATGGCACCATTCACTCTGGACAAAGTTGGGAGACAA
    AACAAAGATGGACTTGTAACTCTTCTCAATGCAGGTGATGATATTTTCCACAATGCTCTTGCCAGGAACA
    AAGTTTCTCTCATGAAGGCAAATCTGCATACCTTCTTTCTCTAGGTTTGGCAATAATTCATTCTTCACCC
    AGAAAGAATCGTGCCCACTATATGAAATAAATGCATGAAACTGGAGATTTCTTTGGAGTTCTTCTAAGGG
    TATGTTCCTGGCCCTGCGCCGGGTCTGGGTCCACTGGCACACCATCCTGAGATACCAGGGCAGATCCAAG
    TAGCTGCAGAGGGAGGTCACAGTCACAGCCAACACCAGCATGGTGGCAACGATGGTGACGATCAGCAGAG
    TTATGTTGCAGGATAATTCAGACATGTGAAAGTCCTTTAGTAGGGTTCCTCTATAACTTTCCGGGTAGTC
    ACACTTATAAGAATCAGGCCAGCCCTCTAACACTTCACTTGATACTTGGTCTATATTTTTGACAAATTCT
    CCTAGCTCACAGGTACATTGGAATGGATTGTCCCCTGCTTTTATTGACCTCATCTTCTGGCAGCTCTGGA
    AGAAATCAGCCGATGGGTGGGAAACTGAATTGTGATCAATGATCAATACAGAAAGGCTGCTAAAGCTGCC
    ACATCCAGGAAGGTCAGTTAAAGAATTGAAAGCAACATTGAGTTCTTGCAAAGCTTCCAGTTTTACGACT
    TGTTTAGGAATGCTCTTTATTTTATTGCTGTGAAGATCAAGTACCTTGATCCTGGGAGGTAAACATCTGA
    AAATAGTGTCAGTAAGTATATTTGAAGACATATTTAAACTTAATAAACTTTTAGTCCAAGAACAGTCTCC
    TTTCTTTTCATCATAGCTTACAGAATTCTGGCTAATATCCAATTGTTGCAGAGACTTCATCTGTGTAGTC
    ATTTCAGCTATTTTTGAAAGTTCTTTTAATTGATTCATTTGTAAAATAAGTGTCTCCAACTCAGTAAGGT
    GCCCACAATTTTCAAAAACCGTGTCTGTTAAGAGATTATTGGAAAAATCCAAATGCAGGAACGGGCTAAT
    TTTGGATGGGCAAAGCATGTGGACCATGCGTGTACCAGACACTGTGAAATTTTTGATGTTCATATTCGAA
    AAGATTTCATAGATATAACTTTGCGGAAAACCGAACACATCGCTGACAACTTGGTGTATAGACAAGGCCT
    TCAAGGAAGTGCCAGAATAATCAAAATCTCTGAAGTCCAGCTGACCCTGTAGCTTCACGTTTGAAATTGA
    GAAATACCATACAGTTGTATGCCAAACCAGCTGGAGGATCCTAATGAAAGAATTCCAAGTTGTTTCAATG
    TTGTTTAAGGTAAGATTTGATAACTTTGGATTTGTTTGAAGTTTCGCCAGAATACTTAGGAAGTAAGAAC
    ATTTGTTATCTTCTAGCACACATTTGATATTAGATAGTTCCAGATTTGCTACAGTCTTGACTGACACATC
    CAAAATAAAATGGAATTCTTTGTTTGTGGGGAACACAATGTGCAGACTCTCAGTGTTAAAGTCTTGAAGG
    CCCTCAGGGTCTTCTTTTTCCCCATAAGTCTCTCCTAAGACCAGCAAGACCTTGCTGATATTCAAATGAG
    CAATTGGCAGCACACTAGATTTTTCTAAGTGTGTGGTGCTCAACCCCAGAAATTTTAGTTGAGACATATT
    GCCAAACTCTTTGCATATAGGCAGGGCATCAAATGCATTAAATGACAGGTCCAAGTGCTTGAGGTTCACA
    GTAGGGTGGCAAGAAATCTTCACCAACTTGTTGTGGGACAAATCCAAGTATTCCAATTCCTGGTTGAATT
    TGAAAACACTGATATCAAGATACTGGATTCTATTATGAGAAATTATCAAAATCCTCAGTTTTGACAGTGA
    TAAGATGTCAGAAGTCCAAAGCTCAGATATATAATTTTGCGATATATTTAAGATTGTTGTTTTCTGGGAT
    AGGTCTTTAGGAACGTGGATGAGACCGTTTTTTGACCTATCAACTAAAAATTCACTTTCTTCAGATAATT
    GTATTCTGATCTGAAGTATTAACATGAAGATAATGGCAAAATGGAAGATGCTAGTCATTTTGGAACACTA
    GACATTCCTAAAGGTAGAAGCTGTTCTTCAGATCATCTTGATACAGATACAGATTCTAGAAAAAAAATAA
    TGAAATGATGAAATACATTACATACATTTTTAAAATACACGAAATTAGTCATGGCTGACATTAAACTTTT
    TTTTGTTACAGACTTATAAAGTGGAGGCTACATTCTTTTGGGTTACATGGCCTAAAACTAGAGAAGTATA
    TATATTGGTAAATAATGATGCCAGCTCTAATCAAATAAGTTTTAATATGGATGGTGTAATAGGTTGAATG
    GTGGCTCACCAAAAGATATGTCTACATCTTAACCATGTCCTAATCCCCAGAAACCATGAATGTTACCTTC
    CTTAGAAAAGGGGTCTTTGCAGAGATAATAAAGTTAAGGATCTTGAGATGAGGAGATCATCCCAAATTAT
    CCAGGTGGTCCATAAATCCAATGACAAGTGCTTTTTTAAGAGAAAGGCAGAGGGATATTTGACAAAGAGA
    CAAGAGAAGAGGCAATTGAGACACAGAGGAGAAGGCCTTGTGACAGTGGAGGCAGAGACTGGAGTGCAGC
    AGCTACAAGGCAAGGAACATCAACAGGCGCCAGAAGCTAGAGGAGGCTCTGGAGTGATGGTGGCCCTGCT
    GACACCTTGAGTTTCGACTTCTGCTCTCCAGAACGTTGAGGGCAAAGCCACCACGTTTATGATCATCTGT
    TACAGCAGCCTCAGGAAACTAATACAGGTGGGTTCATAGAAAGTTTTCTGGCCCCAAGCTTCCCAAAATT
    ATTTAGAACTCCAGCTAAGACTCAACTGGTGTACTTTCACTCTTTGACGTACATCTCCCCATGACTTTCT
    CAAGAGGTGTTCCTAATGGTGGTATTGGGACTGCTCTTCTATTACCCTTTATGACCTATACACGGTTTGG
    GTCTAACTTTGGTCTTACTCAAAAGGTAATTAGATCCTGATAAAAGGCCTCTCAGAAGATCCATTTATTC
    ATCCATTTATTTATTCAACCAGTATTTATTGATCACCTACTGGCCATGTTTTAAGAACTGGAGATAGAGC
    AGTGAACAAGATAAAGTCCGAATTCTCAAGGAGTTTTTATCTAGTGGGGAGACAGAAAATAAATGAATGA
    CTAAACTCAAGTTCCTGTAGTGATAAGTTCTGTAGAGAAAAATAAAGCAGGGTAAATTGTAGGTATGAGA
    CAGTGCTTGGGGGTGGCTATTTTTGAGTGATTAGTTAGAGAAGGCCTTTTTGAGATGATGACACTAGAGT
    AGAAATCAAAATGAAGTGAATGAGTAACCTATGGGAAGATCTGGGTAACCTACAGCATTTCTGGCAAAAG
    AAAAGGCAAGTATAAAGGACCTGAGATGGAATTGAGCTTGGCTTGTCTGAGAAGGAACGGGAAGGTCAAT
    GTTAGTAAGGAGTTAGGGAAGATGAGAGGAGTTAGGGCCAAGATCACTCAGGCTTTGTTGATCATTGCAA
    GGAGTTGGGATTCTATTCCAAAAATGATGAGAGGGTGGAGAACACTGGGAGGTTTTGAGAAAGGGAGTTG
    TTTCCTCCTCTCTCTTATTCAGAATACTTTTTTCATTCATTCATCAAATATGTATTTATTCTCCATGCAG
    TGGTAGGCACTGTTCTAGGTTATGCCTATTGTACCAGTATTCAAACTGCTCTAGCTAAGACACCATTTTG
    TGGTCAGCATTCCAGTGGCGTATGAGAACAGAGGGAGCTGTGTTGGGATGAACTCTAGAGTGTGCAGTCC
    TCACCTCCAGAGACCCTTTCAGCCGTGATTACACAGCTACCCAGTTCTCACAAGGAACTTATTCAAAATC
    CCCTAGAACCATTAGACAAGCAGGGCATTAGTATCAGCAAAGCACCAAATTCCTCTTCACATATCCCCTC
    CCATATCTCAATGAGCACAGAGAACCTAAATAATTTAGCTGCTACAAACAACCCTCGGTTACAAAAGTTC
    CCCAATTTAGATGTGCAAACTTATCTGGGTTTTGGAGCCTTCCTTGGACTTACCCTTTTGTAGGGGTGCC
    CAATATGCCTTTGTTATCCTGATTTCTTCTAACGAGGAAGAGGGCCTGGTACCCCTATTAGTGTTCATGA
    AGACCCTGAGCAAGGAAGATGATGCAGACATTAATGTTCATGTTAAAAGTCAAGGTGGTGTTTTTATGAC
    ATTAACTGTATTTGGCTGAAAGACCCGGAAATTTCCCAATTCTTCCTCTCCAGCTTCCTTAAAGAGCTAC
    TCACACAAGGAGCAATATTTCAATGGATGTTATAGCTTGAATGTTTGTGCCCTGTGAAATTCTTATGTTG
    AAATCCTAACCCCCAAGGTGATGGTATTAGCAGGAGAAAATTGTGAAGTAATTAGGTCATAAGAATAGAG
    CCTTTATGAGTCAGATTAGTACCTTTATAAAAAAGACCCCAGAGAGCCTCAGGCTGTCATTCTGCCATAT
    GAAAATACAAGAAGGAGGCATCCGCAAACCAGAAGAGGGCCTTCGCCAGAACCCAACACTACCACCCTGA
    TCTTAGACTTCTACCTTCCAGAACTGTGAAAAGTAAGTGTCTGTTGTTTATAAGTCACCCATTCTGGTAC
    TTTGTTACAGCAATTTAAACAGACTAAAATAATGGGAATAAAAATAATACCCTGGGGCAGTGCCAAGCTA
    GGTCGAAGCAAAAGTGGCCTGGTCTTAAGGCCTAAGTGGGGTTCCCTGCTTATTCCTATGCCTGAATCCA
    GATCAAGGCTCTTTCCACCTCCTAGCCAACTCCAGAGTTCATGGAAGCAGTGATCAGCACAGTCAGGCGT
    GGTATTCAAAAGTGATCAGAGGCACACAGGACCCCGTGCATGAGTGCGAGAGCTTGGCTCGCAGAACCTA
    CCACACGCAAGATGAAAGTTGTCCTTTTTCCCTTAACAACAGCTATCATCACAAAAATATATAAATTTAT
    ATAACTGCCTGCCCCCAAGTGTTAGAGTAGGTACTTAGGCAGACATGAGCAGGACAGGAGAGGGGCCCCC
    CAACCAGGAATATCAGGTGACCATCAGGTGATGATCAGGCAGTTGTTAAACAGTCTCTCTAAGCCGGGGG
    CCATGGCTCACGCCTGTAATTCCAGCACTTCGGGAGGCTGAGATAGGTGGATCACTTGAGGTCGGGAGTT
    TGAGACTAGTCTGGCCAGCATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGATG
    GCAGGTGCCTGTAATCCCAGCTACTGGGGAAGCTGAGGCAGGAGAATTGCTTGAACCCAGGAGGCAGAGG
    TTGCAGTGAGCCAAGATCATGCCATTGCACTCCAGCCTGGGCAACAGGGCTAGACTCTGTCTCAAAAACA
    AACAAACAAAAACAAAAAAACAGTCTCTCTAGAATAATAATTGTTTGCAGCCAGCATCAGGGAAAGTCAG
    TCTCCTAATAGATAGAAAACACACCTGAAGCTGGTGGTTAGCAGACTCCTGACAAACTCTCAGGAGCTGG
    GCAAGCAGGCTTGAGCATGCATACCAAGAGGCAAAATGGCGGACTTCAACGGGTATGTGACCTTCCTCTA
    GGAAGACTTGTCTGGCAAGGGAAAAATGTCTCCAATGAGCATGTACACAACTTCAGTAAACACAGTGTGT
    GTGCAGCCCATCCCAAGGGCTGGCAGGCCACTGCACATGCAGACAGCCCACCCCAAGGGAAGAATCAGGG
    GAGAAGAGACGCAAGACTCCAGAAGCCTGCCAACAAATAAGCCCCAAGTAATAGGTTAAACTGGGCACTT
    GGATCTCTCAAGTTGCCCACTTGGCCCTCCTCCAAGTGTACTTTATTTCCTTTCGTTCCTGATCTAAAAC
    TTCTTAATAAACTCTCTGTAAGGAATATGGCTGTGCTTTGGTCAAGGATAGGCCAAGGTAAACATCCAGA
    GTGACTCAGCGAGTTTAGAGCGCAGGCGTATAACTCACTTTTTATCATAGCCATGTAGACATAACATAGA
    GAAGCTTACCATAGCCATAACATAGGGAAGGTTCATCCCTTGGCTCTAAGCCACTATTGTCCGTAAATGG
    TATAATTGCCCTGCTGACGCTGTACAGGTGCATTGGCAGCCAGAGAAAGAGAGAGAGAGAGAGCCAAAGC
    TGTCCATCTTTGCAGATGGACTGAGGGGAGCCAGGACACAGCTCGGCTTGCTTGTACCCAGAGAGAGAAA
    GAGTTAAGCTGCTGACCCTGAGGGCAAGGGAGAGCCGGACGCACAGTTGTGTGTGGAAGCTGCCGAACTA
    AGCAGCCAGGACAAGGCGGACAGTCTGAGAGAACTACTGTAAGTAAGCTGCTAATGAGAGCTGTTGCTGA
    ATAAAATCACCTTTCACCTGCCTACGGCCCCCTGAGTGTTCTTTCAGCTATCTGCTCATCCACCCCCTCC
    CCTCAGACCTCAGTATGGGCTGGAACCTGGCCCTGGGCGTGACACTCTCTCTCTTACTCTAAAACTTGCC
    TCGGTCTCTCACTCTGCCTTATGCCCCTCACACAAATTCTTTCCACTGAGGAGGCAAGAATTAAGTTTCT
    ACGACCCATATGGATTCACCACTGCTAACGCAAGCATTTAATGAAAGCATAGCTTCAGTTTTATGTCGAG
    CTAAATTAATTGACTCAAATATCTTCTATTTGCCTAACCTCCATCTTGCCCTTTCATTTAAAGTCATTAA
    TTTTAAGTACTCAGTTTATGAAACCATTCTTATTTCTATAATTAAGAAATCAAAATGTTATACACAGGAA
    TAACTGATTTTTAATACTTCCAGGATCTTTGAATTTTATATACCAAACTTGCTAAATCCATGAAAATCAA
    TGTCTCAGTTTCCTAGACCAAAGAATTTTTTGATCAACGTCTTAATGACCATGACTGAGCCTCATCTATT
    TTAGAACCTTTAAACTCTTTTACTATCCAAAAAATAAGCTGAGCAGAAAAGGATGCTTTAATTTGAAAGT
    GTAAAATGCCTCTAGGTGAGCTTCTGTGAATTTTCATTATTATGATAGGTTTAGCCTCACATTATATGAT
    ACAATGAATCAATACATTTAATTTGTCTTCTTTTGGAGACTTTGGAGGCATCAAATCAAGGCTTTGACTG
    GTCTTAATGGACAATACCCATACAATCTATCTGGCCCATTAAGTTTAGTCCTGTAAATATTTACTAAGGG
    CCTTCTCTGCACAAGACCAAATAGCTATTAACCAAAGGTTAAAAGAATCTGAGACAGTTCAAGAACTGTT
    CTGCTTTGCTTTCTATGCGGTTTTGTGAACTCTTGCTTTGTTATAAAACAAATTCACTTTCAAGTCTTTT
    GACCAAATTGGATGTAGCCTGGGAAACATTTTCAAAACCTGCAATTCTACCTCACGTCTAGCTCCCCAGA
    ACAGTGTTAGTGGCCTGAGAAACAGAAGGACTAGCTAGTGGGAAGGCCCCAGAGAGAAAAACTGAAACAA
    ACCTGGCCACAAAAACAGAAGAGCTGAACAGCAGCATTGCCTCCGGGAGTAACTGACTTATATCTCATTT
    TCTTTAACACTGAGAAGAGAATACAAATGGCGGGATTAGGTGAAGAGAAATGCTGTCAAAATCAACAACT
    AGCAGCTGCTCTGGAAGAAAACCAGAAGACGTGTGTGACCCAAATATTCATTTCCCAACTCAAAAATATT
    TCCTATAAGCAAATCACACACGAGGTGTTATTCCCAGAGGAGTGTGGGGACTTTCTCTGATTTAGGACTC
    AGAACTGATACACAAATGCCAGTACAAACTACACAGTAACTATATACATATCAGAAATATAACCCACTTA
    GAAAAACACCCAGGACTTACACAGATTTTTGAGACTTCTGTACTTAATAACTAGCTATACAATTATTTTC
    TTTTACAAAAGAAAAGAATGTGTTGCTTACCAAGACAACCTGCTTGTCTGTTCCATTTGGCAGTCTG
  • Protein Sequence : Show Sequence
    >gi|41350337|ref|NP_003254.2| toll-like receptor 1 precursor [Homo sapiens]
    MTSIFHFAIIFMLILQIRIQLSEESEFLVDRSKNGLIHVPKDLSQKTTILNISQNYISELWTSDILSLSK
    LRILIISHNRIQYLDISVFKFNQELEYLDLSHNKLVKISCHPTVNLKHLDLSFNAFDALPICKEFGNMSQ
    LKFLGLSTTHLEKSSVLPIAHLNISKVLLVLGETYGEKEDPEGLQDFNTESLHIVFPTNKEFHFILDVSV
    KTVANLELSNIKCVLEDNKCSYFLSILAKLQTNPKLSNLTLNNIETTWNSFIRILQLVWHTTVWYFSISN
    VKLQGQLDFRDFDYSGTSLKALSIHQVVSDVFGFPQSYIYEIFSNMNIKNFTVSGTRMVHMLCPSKISPF
    LHLDFSNNLLTDTVFENCGHLTELETLILQMNQLKELSKIAEMTTQMKSLQQLDISQNSVSYDEKKGDCS
    WTKSLLSLNMSSNILTDTIFRCLPPRIKVLDLHSNKIKSIPKQVVKLEALQELNVAFNSLTDLPGCGSFS
    SLSVLIIDHNSVSHPSADFFQSCQKMRSIKAGDNPFQCTCELGEFVKNIDQVSSEVLEGWPDSYKCDYPE
    SYRGTLLKDFHMSELSCNITLLIVTIVATMLVLAVTVTSLCSYLDLPWYLRMVCQWTQTRRRARNIPLEE
    LQRNLQFHAFISYSGHDSFWVKNELLPNLEKEGMQICLHERNFVPGKSIVENIITCIEKSYKSIFVLSPN
    FVQSEWCHYELYFAHHNLFHEGSNSLILILLEPIPQYSIPSSYHKLKSLMARRTYLEWPKEKSKRGLFWA
    NLRAAINIKLTEQAKK
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG)
40. TNF-alpha
  • Gene Name : TNF-alpha
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 21926
  • NCBI Protein GI : 7305585
  • Genbank Accession : AB039224
  • Protein Accession : NP_038721
  • Taxonomy ID : 10090
  • Chromosome No : 17
  • Gene Starting Position : 35199389
  • Gene Ending Position : 35201995
  • Gene Strand (Orientation) : -
  • Protein Name : tumor necrosis factor
  • Protein pI : 4.75
  • Protein Weight : 24010.47
  • Protein Length : 235
  • Protein Note : Also known as DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434
  • DNA Sequence : Show Sequence
    >gi|372099093:35199389-35201995 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J
    CTTTATTTCTCTCAATGACCCGTAGGGCGATTACAGTCACGGCTCCCGTGGGGAGCAGAGGTTCAGTGAT
    GTAGCGACAGCCTGGTCACCAAATCAGCGTTATTAAGACAATTGGGTTAGATAAATATTTTGTTTTAAAC
    ATAAGCAAAAGAGGAGGCAACAAGGTAGAGAGGCCAGGTGGGGACAGCTCAGCTCCGTTTTCACAGAAAA
    CATGTCTGTCTGAAGACAGCTTCCCACACTGGGTCCTCCAGGACACCCCGGCCTTCCAAATAAATACATT
    CATAAGCAAATAAATAAATAATAAATAAATAATAAATAATAAGTGCAAATATAAATAGAGGGGGGCTGGC
    TCTGTGAGGAAGGCTGTGCATTGCACCTCAGGGAAGAATCTGGAAAGGTCTGAAGGTAGGAAGGCCTGAG
    ATCTTATCCAGCCTCATTCTGAGACAGAGGCAACCTGACCACTCTCCCTTTGCAGAACTCAGGAATGGAC
    ATTCGAGGCTCCAGTGAATTCGGAAAGCCCATTTGAGTCCTTGATGGTGGTGCATGAGAGGCCCACAGTC
    CAGGTCACTGTCCCAGCATCTTGTGTTTCTGAGTAGTTGTTGAAAGCTCTGAGCACAGAGTTGGACCCTG
    AGCCATAATCCCCTTTCTAAGTTAGAAGGATACAGACTGGGGGCTCTGAGGAGTAGACAATAAAGGGGTC
    AGAGTAAAGGGGTCAGAGTGGGGGCTGGGTAGAGAATGGATGAACACCCATTCCCTTCACAGAGCAATGA
    CTCCAAAGTAGACCTGCCCGGACTCCGCAAAGTCTAAGTACTTGGGCAGATTGACCTCAGCGCTGAGTTG
    GTCCCCCTTCTCCAGCTGGAAGACTCCTCCCAGGTATATGGGCTCATACCAGGGTTTGAGCTCAGCCCCC
    TCAGGGGTGTCCTTGGGGCAGGGGCTCTTGACGGCAGAGAGGAGGTTGACTTTCTCCTGGTATGAGATAG
    CAAATCGGCTGACGGTGTGGGTGAGGAGCACGTAGTCGGGGCAGCCTTGTCCCTTGAAGAGAACCTGGGA
    GTAGACAAGGTACAACCCATCGGCTGGCACCACTAGTTGGTTGTCTTTGAGATCCATGCCGTTGGCCAGG
    AGGGCGTTGGCGCGCTGGCTCAGCCACTCCAGCTGCTCCTCCACTTGGTGGTTTGCTGAGGGGGGGGGGG
    AGGATTGAGTCAGTGTCACCCTCTTAGTTCACACTCCACATCCTGAGCCTCAGCAGCTACCCACACTTCA
    CTTCCGGTTCCTGCACCCTCTGTCTTTCCACATCCCATTGGCTATGAGGTCCCGGGTGGCCCCCTGATGC
    CTTGCTTTTGAGTCACTGCTCTGACTCTCACGTGCTGTCTCTAAGAGCTCTGTCTTTTCTCAGCCTGGCT
    CGACACCCCTCAACCCGCCCCCCAAAATCATGCCCCTTCATTCTCAAGGCACATGTAAAGAAATCTTACC
    TACGACGTGGGCTACAGGCTTGTCACTCGAATTTTGAGAAGATGATCCTGGAGGGGAAGAGACAAAGGCA
    AGGATGAGCCTTTTAGGCTTCCCAGCAAGCATCTATGCACTTAGACCCCTTTCCTCCCAAACCAAAGCTT
    TAAGTTCTCCCCCCACCCCATCTCATCCCATGCCTAACTGCCCTTCCTCCATCTTAAATTAAGAGAGAGG
    TGTGGGAACACTTACTGAGTGTGAGGGTCTGGGCCATAGAACTGATGAGAGGGAGGCCATTTGGGAACTT
    CTGTGTAGGAAAAGGAGGTTAGTTAAGACAGACTCACCCCAAAGGAGAAGCCTCCCGGCTGATTGCCCCG
    CTTACAGTTCCTCTTTGCCCCACCCCACCCCCCAGCTTTGTGTTTTTCTTCTTCATTCATTCATCTGTCC
    AACCCACGGCTTCTTTCTGCGGTGCCCTCTGTGCTTGATCTCCCGTTATCTCCCCTTCATCTTCCTCCTT
    ATCTCTCATGCCTCTCTCATTTCTGTCTCTGAGTTTTATCTCTTGCTTATCCCCTCTTCCCCTGGCCACA
    TCTTTCCAGATCTCTCCACGTGTGAACACACTTGTTCGTTCATTCATCTCTCTGTGCATCCGACGAAGGA
    TGTTTAGTCAGCTGGACGCATGGGTCCGAGGTCCTGACTCTGTCCCCTCCACACTCTCCTCCACCTTGCC
    CTGCCCATTAGCCCACTTCTTTCCCTCACACTGTCCTTCTTGCCCTCCTAACCCGTTTTGCTTGTGAGCG
    AGAATAAGGGTTGCCCAGACACTCACCTCATCCCTTTGGGGACCGATCACCCCGAAGTTCAGTAGACAGA
    AGAGCGTGGTGGCCCCTGCCACAAGCAGGAATGAGAAGAGGCTGAGACATAGGCACCGCCTGGAGTTCTG
    GAAGCCCCCCATCTTTTGGGGGAGTGCCTCTTCTGCCAGTTCCACGTCGCGGATCATGCTTTCTGTGCTC
    ATGGTGTCTTTTCTGGAGGGAGATGTGGCGCCTTGGGCCAGTGAGTGAAAGGGACAGAACCTGCCTGGTT
    GGCTGCTTGCTTTTCTGGGAGCTATTTCCAAGATGTTCTGGAGTTTCTGTTCTCCCTCCTGGCTAGTCCC
    TTGCTGTCCTCGCTGAG
  • Protein Sequence : Show Sequence
    >gi|7305585|ref|NP_038721.1| tumor necrosis factor [Mus musculus]
    MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRDEKFPNGLPL
    ISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQ
    VLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQL
    SAEVNLPKYLDFAESGQVYFGVIAL
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Bacille Calmette-Guérin (BCG) , Mycobacterium tuberculosis fadD26 mutant vaccine , Mycobacterium tuberculosis mce-2 mutant vaccine , Mycobacterium tuberculosis sigE mutant vaccine
41. TSC22D4
  • Gene Name : TSC22D4
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Gene ID : 81628
  • NCBI Protein GI : 13569901
  • Genbank Accession : AC092849
  • Protein Accession : NP_112197
  • Taxonomy ID : 9606
  • Chromosome No : 7
  • Gene Starting Position : 94697542
  • Gene Ending Position : 94710214
  • Gene Strand (Orientation) : -
  • Protein Name : TSC22 domain family, member 4
  • Protein pI : 7.24
  • Protein Weight : 39140.01
  • Protein Length : 395
  • Protein Note : Also known as THG1; THG-1; TILZ2
  • DNA Sequence : Show Sequence
    >gi|157734172:94697542-94710214 Homo sapiens chromosome 7, alternate assembly HuRef, whole genome shotgun sequence
    ATTCAAGTTTCTTCAGAGTTTTTAATTATTATTATTATTTTAATAACAACCTGAATCCCAGAACTTCCAG
    ATACTGTCCACACTCCCTCCCCTGGGCAGAGGAGAGGAGGCACCTCAAGGGAACAAGATGGGGGTGGGGT
    GTCTGCCGGGGAGCCTCCGACTCCCCCAAGCCGTCTACTGCTGGGGGGTCCCAGGAGGGAGGGTGGGGGT
    TGGTTCCCTCCCAGAGGGGGCTCCCTCTGACGCCCCGTCCTGAAGCCCTCCCTCCTCCATCAAGGCTGGG
    GATGATGAGGAGATGGGGCAGGGGCCGGGGGACCAGGCCATTACTGAGCCTTGAACTCCCACCCCGGGGA
    CACGGGGACATTAAAGCTGCATAGGAAGAGGGGGCAGGCGGCTGACGCAAGGCCGGGCAGCCCCAAAGGC
    ACATTGTAAGGGAAGGGAGGCTCAGACGGAGGGCCCATTGGGCGCAGGGGGCCCAAGCCGTGGGACCCCC
    GAGGAGGGCAGCTGAGCCAGCTGCTCCGGGCTGGCCAGGGCGCGCAGCAGCCCATTCTCCTGCTCCAGCG
    CAGCGTTCCGCTCCGCCAATTCCCGGATCTGCTCCTTCAGCACCTCCACCTCCTCCCGGACCGCAAACAT
    GAGGTGGGACTTCACCAAGTCCTGGGGGCCCCGGGACAGGCACAGCGTCAACGGGGTGGGTGCCTCATCC
    CCTGCCCAGTGCCTTGAGGGCTGGGGTGGGAGACAGTCCCCAGTGTCCAGAGGATGAGGGACAAAGCAGG
    GAAGGGACAGGGGAAAAGGACGAGGGACAGAGGCAGAGTCAGGGCTCAAAGACAGAGATGGGGACCAGTA
    GCCAGACTAGAGGCTGGGCAAAGTCAAGGGTGGAGGTGGTGGCAGGAGGGGGCAGAGGATGCCCAGCAGC
    CCAGCAGGGAGAGGGACGGAGGAGCTGGGAGTTGGTGTTCCCTGGTAAGGAAGAGGACAGGGCTGGGGCA
    GAGGGATGGCTTAAGGGGGCCAGGACCTCCTGGGACCAAGATGGCTTCTCTTACCATGGCTTGCTCGATT
    TTGTTGTCAATGCCAACCAGGCTTCCGGAGCCACTGGAGAGACACAGGAAGGTGAGGGGAGGAGAGGAGA
    AGCCTTCCCAGGTGCTGGTTTCTTGGACCACAGCCTCCCGCCCACACCCCCCTGTACCTGTGACAGTAGA
    GAATGAGGGAGAGGAGGGTTTTGTCCATCCCCCTGCGGGTTGAAGGCCGCTGCCCAGGGCCTCCCAGGCC
    CCGGTGGGGCGGGGGCCCAGATGTCCCTCGGATGGGGGATGCACCCCGGGGAGAGCAAGGGGAAGAGGCA
    CAGCCCCTTTTTCGGGGCAGCCCCGGAGCCATCCCCACCACTCGGGTCTCATAAGACGACACCAGGGACT
    TGACAGAGACCCCTGGCTGGGCCATGCCCAAGGGGAAAGGGTCCTTAGACACCCCCCCAATGGAGAGGGA
    TGCAGTGAAGGGAATGCCTGGGGGGCTCAGAGCCCCCCGGGCAGAGCAGGGTCTGGCTGGTGCCAGGCAG
    GCAGAGAGGAGACAGTGCCTCGGGGGTGCTGGCCTGAAAGATATACACCACCCCCCCAAGGGCAAGGCCT
    GGCTGCTATTGGCTCCTTGGGGACACGGGGACCCCCCCTCCTTTCAGCTCCTCGGTGGGCGGGAATTCCC
    AGACACGGTCAGTGCCAGTGTCCCCTTCTCACCCTGGCACCAATCCCAGAGTGGAGGGGAGGGGGCCGGC
    CGAGGAAGTCAGCTGGTGGCTGGGCCAGGGGAGGGGCGTGGGGCAGAAGCCTGGGTCTGAGCGGGGAGTG
    AAGGGTGTGAAGGGAGAGGAAGGGGCTGGCCTTCGGAGGCCCCTCGCCACCTCCGACCCCATTCTGTCCC
    CTCCGTCACTCAAGGCCTTGGGAAAGAGGGTGGAGGTGGCTGGGGTAGGGGGCGTTCTGACGTGCTCGTG
    GTCTTCCATAGATCATGAGCGCTCTCCCACCAAAAAACCCCACAAGGCTCACATCATGGGTGACCCCTGG
    GATCTGGCCAGAGGGTCCCTGAGTCTCCAGAGAAGACCAAAAATGGGAAAGGTTACCATGGCAACAGAAA
    CTGAGCCCAAAGCCCTAGAAGATGAATACAGCGTAAAGGCCAGGCGTGGTGGCTCACACCTGTAATCCCA
    GCACTTTGGGAGGCCAAGGTGGGTGTATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGA
    GAAACCCCATCTCTACTGAAAATACAAAAATTAGCCAGGCGTGGTGGAGGGTGCCAGCAATTCCAGCTAC
    TCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGTGGAGGTTGCAGTGAGCCGAGATCGTGCTA
    CTGCACTCCAGCCTGGACGACAGAGTGAGATCTTGTCTCAAAAAAAAGCCAGGTGTGGTGGCTCACGCTT
    GTAATCCCAGCACTTTGGGAGGCTGAGGCGAGTGGATCACCTGAGGTCAGAAGTTTGAGACCAGCCTGGC
    CAACATGGTGAAACCCTGGCTCTACTAAAAGTACAAAAAATTAGCTGGGCATGGTGGGGGGTGCCTGTAA
    TCCTAGCTACTCTGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCGA
    GATCGTGCCACTGAATTCCAGCCTGGACAACAAGAGCAAAACTCTGTCTTAAAAAAAAAAAAAAAAAAAT
    CGCCTATGGGCCTGGGACCTGGGTCTGGGGTCTCAGAGGAAGCGAGGGTTTGAGGAGAGGAAGGGAAGTG
    AGCTTGGAGAAGGGACACTGGGAAGAGGAGCAACAGGGCTTGGAAGAGGGCTGGGAGTCTGGGGTGACAG
    GGTCTGGCTGCAGTCTGGGAAGTGGCCACCAGAGGGCGACTCAGGCCTGGCTCAACAGCCCCCAGGGCCG
    GGCTCCAGGCTGGCTCCACGACGTTGGGCTCCTGGTCCCAGAACCTGGCTCTCCCTCAAGCCCCACTCTC
    AGGCCCCACCTGCGCAGGCCCTTGTGCTGACCTCATCCTCCAAGGCTCCCTCTCCCACCCCTCAGGCCCC
    AGGCTCTTGCCCTGAGGCTCCCGACCCACCCTTGCACCCCTGCACTCTGGCGCTGTGGCGGGATCCCCAG
    TCCTGCCCTCCAGCCCCCAGCTGGGCCCCCCTTGGAGCAGGGGCCTCAAGGCGCTGCAGCCCACATCCCT
    GGACTCCCTTGTAAGCCCTCCCTCCTGGCTGTCAGCTCAGACTTGCTTTCCTGCAGGGGGATCAGCACTC
    AATTCAAGGTGACCCCTGGATAAGGTCCTGCAGCCAGGAAGGGAGCACAAGGGAGCTGGGAATTTTCCAT
    TTCTCATCCCAGCTCTGGGTGCAGAAGGCGCTGACAGGTGGAGCAACTAAGGAGGTGACTAGGCCTGAGC
    GAGCAGGATGGGGTGGGTGGGGGAAGGGCATCTGGGACTCTTGGGGTGGTGGGAGAGGAGAAGTTGAAGG
    GTGGAGGTGGGTCTGCCAGCAGGAGGGTCAAAGTAATTTTCGAGCTATTTCAATCAGCAACACCTGGGTG
    GAGATGGAGCCACCACAGAAAGCCCAATACTGCCACCACCCCATTAAACACTCACAGAGACCAAAGGGGT
    TGGATACACAGGGATAGGAACATGGGCCCTGCTGGGCAGAGACTCAGGGGTAAAGATGTCAAGTAAGGTG
    TTCATTCTCACCAGAGATAAAGGAATAATACAGCAATAAACGTTTGTTAGCCGACAGGTGACTGTCTTCT
    GTTTGTCTAGCGCCTGGGAGTTTTTGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGGGATTTTCCCTCA
    TTGGAACCTCCACCTCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCAGAGTAGCTGAGATTACAGGCA
    TGAGCCACCATGCCCAGCTAATTTTTGTATTTTTGCCGGGGAGCCTCCGGCAAAATGGGGTTTCACCATG
    TTGGCCAGGCTGGTCTTGAACTCCTGACCTCATGATCCACCCACCTCAGCCTCCCATCTCAGTCCTGGGA
    TTACAGGTGTGAGTCACTGTGCCAGGCCTCTATTGGAACATTCTAACACACACTTCAAGGGACCAGGGCT
    CTGGCAATGCAGGCCCTTCCCTTTAGGACCCCAAAGCGAGGGGTTGTGGGCACTGTGCTAGAATTCTTTG
    ACAGGGTCCGGGGGAGGAAAGTAGGGGCTGCTGAGACAGGATCGACCTCCACCACAAGCCCAAAAGCAAG
    TTTGTATGGATTAGAAAATGCCCCCTTCTTCGAGATCCTTAATGCCATCTTCTGGAAAACTGTCGTGACT
    TCAAGCATTGACTGTGATTTCAAATATGAGCGGCACCCCCTGGAGTTAGTTGTGCAGTGTGTGACATGCA
    CAGTGGGAGACTTAGCCCTGCAGGACTCTTGGTCCTGGGGCGGGGACAGCCCTAGAAGCTGGTCTTCCTC
    TTTGAGGGTCAAATAAAACGGCCTCAACATGAAGATGAGATAAAAAGCAGGACACCTGGGAGAAATGGGG
    AGCCTGGGATCTTGGGGACAGGGGCCAGCCCTGCACACTCTCCAGTGGGTTCAGGGGATCCCCCAGGGCC
    TAGGTCTGGGGCCAAGAAAGTGTCATCCAGCTGCCCTGTCTCTATGCTGTCCCGGATCTGTGGTGTCTTT
    GGGGCGGGGCTGGGGAGGTCGGGGAGGGGGAAGGGGGGCTGGCTCCCTGCCCTGCTTGGGAGAGCTGTAC
    CTGACTGTCCTAGAGAGAAAGGATATTGTAGACCATTGCCGCCACCTCTTTGCCTGGGACACAGGCATCC
    CAGTAGGACAGTGGAGAAGGTGTGGCTTTGGAGTTGGACCCAGGCTCCAGCTGCAGTCCAGCCACTTAAA
    CTAGCTGGGTGGCCCTTGGGCAAGTTTCTAAAACCCTCCTGGCCGGGTGAGGTGGCTCATGCTTGTAATC
    CCAGCACTTTGGGAGGTCGAGGTGGGTGGATCACCTGAGGTCAGGAGTTTGAGATCAGCCTGGCCAACAT
    GGTGAAACCCCGTTTCTACTAAAAATACAAAAATTAGCCGGGCATGGTGGCATGCACCTGTAGTCCCAGC
    TACTGGGGAGGCTGAGCCAGGAGAATCGCTTAAACCTGGGAGGCAGAGGTTGCAGTGAGCCAAGATCGTG
    CCACTGCATTCCAGCTTGGGAAACAGAGCAAGACTCCATCTAAAAAAGAAAAAAACAAAAACAAAACAAA
    AAAAAACCTCCTTCTCTGAGTCTCAGATACTTCATCTGGCAAGTGGGAGAATGGAACTGCTTTTTAGGAT
    TAGAAGGGAGAAGGCAGTACCGGACCCAAGGGAGGCACTGGATAAATAAATGTACCTTTTCTTTCCCAAG
    TCCCTGGGATCCATTCTGTATAATGCCTGGATCCCCCAGGCCGGTCACTGCCCCCACCCCCATTCCTTCC
    ATCCACTTCTTCACACCTTAATTACCTCCTGCCTGCCGGGAGGGGACAAGGATTTCCAGCCCTGGGGCAC
    GTGAGCATTCTAGATTCACCTGCACCCTCTGTTCGCCCATCCCCAGATTCAGGGACTGGTCAGGCGTCCC
    CAACTCCCTCCCTGTGATCCAGGCACTGAACATCTCAGCCCACCTTCCCTCTTAAGAGAGAGGTCCCAGG
    CCACTGGCATCCTGTCCTGCCAGCCCTGTGCACACCAGACAGGAGAGGTGGGGCCTACATGTCCCATTCC
    TAGGGGGCAGGGGGTGGCCCAACCCAAACCCGCTGGCCTGGCAGGGTGGGGCTTGAGGGGCTGGGCTGGG
    ACAGATCCCCAAGAGCCTTGGAGCACAGCAGGGTGAAGTGGAGGCTGGGAACCGGACCGGTCAGATTGGG
    GTGTGCAGGGAGGGGGTGGGATGGAGGGAGGCAGGGAGGGAGGGTACAGGGGAAAGACGGAGGCCTCAGG
    AGGTTGAATCCTCTAAGACAGAGACAGCAGGAGGCTGGGCTGTGAACTCAACAGGGGTGGGGGAGGGACG
    GGGAGTCCTCTTGCCGGGGAACCTCCCAAGTGGGAGAGGGAGAGGGCGGGAAACGCTGAGAATAGCAGTG
    ATGGCGGCAGGTGACTCTCCCAGCCCTGGCCGACCTCACACCTCGCACATTCCTGAGAGTGGGAATGGGG
    CGTGCAGAAGCCACCCAGTCCTGCCCAGCCCCGCCCAGCCCCACCCAGCACTGGCACTCTTGCCCTCCGT
    CCCACCAGCTCCTGGCCACCTCCTTTGCCAGGTCCCTATCCTCCCTGAGGCCGCAGGACCCTATCAGTGG
    TGGGCTTGAGTCCCTGATCCCAGCGAGTGGCCCCTGATCTGGGGAGGACAGGAAGGAGCGACTCCCATCT
    GGAATTGGTCCCTCAGGCACCCACTCACCCACTCTCGAAGCCCCACCCCCAATTTCCTTCCAAGCAGGAA
    GTCCCTCCCCTTGAAAACCTCCCACGGGAAGTCCCACTTCTCCCTCCAAAAGAAAAGCGGTGACTCCCCA
    GCCCTTCTGCCCGGTTCCTATCAGTCCCCAGGGAGAAGCAGGCAGCCTGGCTATCTCTGACTCACGATTC
    TGGGCCTCCCTTTTCAGCTTGCTGGCTTTCTTAGGAATGGTGACACCTGGCCCTGAAAACCCACCCAACA
    GAGCACTGTCCCCTAAAGGGAGAACCCCAGAAAGTCGCTCTCTGCAGTGCTGCCCCTGCCCCTTTGCCGT
    TCCTAGCTCTGATTCTGTTCCTAGCTGGCCCATCTTAGGGACTTGCTTTCTCGGTTCTAGAAATCTTAGG
    ATCCTAAAGTAGACCCTGTGGGCTATCAGGATGTCACGCTCCACAGGTGCTGTTAGGGAGTCCCAGTCCT
    TTGTTAGGAGAGAAAGAGGGAGATGGAACTGGGAGGAAACTGATTTCCATTCTTTTTCAGTTTTTTTTGT
    TGTTTTTTTTTTGAGGCGGAGTCTCGCTCTGTCACCCACGCTGGAGTGCAGTGGTGCGATCTCGGCTCAC
    TGCACCCTCTGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCA
    CGTACCACCACGCCTGGCTAATTTTTGTATTTTTAGTACAGACAGGGTTTCACCGTGTTGGCAAGGCTGG
    CCTCGAACTCCTGACCTCAGGTGATCTGCCCGCTTCAGCCTCCTAAAGTGCTGGGATTACAGGCATGAGC
    CACCATGCCCAGCCTACTTCCTTTATGTATATATATATATATTTTTTTTTTTCTTTCTTTCTTTTTCTTT
    TTAGAGATGAGGTCTCGCTACATTGCCAGGGCTTGGTCTTGAACTCCTGGCCTCAAGCAATTTTCCTGCC
    TGGGCCTCCCAAAGCACTATGAGTACAGGCGGGAGCCACTGTGCCCCGGCCTGATTTGCTATTCTGTCTA
    GAGCATGCATTTGCCAGTGGGTTCCTGGGTTGGCCAGATGATCTCTAAATCTGCAAGAGCTCTGGAATCT
    GACAACAATGACTCCCTTCAGACTCTAAAAAAGTCTGTGACCTGACAGTTCTGAGCCAGGGAGTCCCACC
    CAGCCCTCTCCACAGAGAGGGAGTGGGTCCGAAATCAACTGTGTGCAGTGGCTATGCCCAGGCCAGGTTT
    CTCTAAGAGGTCCTCTCCAAGTTCAACCTGGGGGAAGGATGCCTACCAGGCACTTTCTTACAGCTACCCC
    GGGTGCTGGGCGGGGAACCTGAACCCCACGCCCCACCACCCCACGAAGCCCACCTACCTATCATCGTCGC
    TGTCTAGGTGACCACTGATGGCCAACATGGAGTGGGCCAGGCTGAACTTCTGAGCTGCTACTGCTCCGAA
    GGGGTCTGGAGATTTGTGAACCAGGCTGGCATCGTGGGTGAAGTAGAGGGCTGGGGAGCTGGGGCGAGAG
    TCAAGTGGGGGCACCTGGAGGCGGAGAGGTAGGAACCATCACATGAAAAGAGAAAAGAAAGGAACCAGCT
    GCCTTAAAACTTGCCTATTAGCCTTCCTCCCTCTCCACCTCCCCACTCTCTTTCGAGGACCCAGGAGTCC
    TGTCCCCGCAGTCCTTCCCTCCTCCAGCTCTGGAGAAGGCATTAGGCAGGTACTAGAAGCAAGAAGTGGG
    TCAACCTAAGGAATGGGAGGAGTGGTGTATAGGACATTAGAGAGAGTCTCCCAAGTGGGAGGAGGGGGTG
    ACTGACGGAGGAACCCAGAATAGACTCCAATGCCCCAGCTCCCACTGGGCCGGGACCCCTAATCAGAGGC
    TACGCAGCGTGTCTGCCTTCTTTTATGTTTTATTTTTATTTTTTTGAGACAGTCTCACTCTGTCACCCAG
    GCTGGAGTGAAGTGGCACAATCATGGCTCACTGCAGCCTTGAATTCCTGAGCTTAAGCGATCCTCCAGCC
    TCAGCCACCCGAGTAGCTGGGACTACAGGCATGCACCATCATAACTGGCTAATTTTTTATTTTTGGTTAA
    CACAGGGTCTCAAGATGTTGTCCCGGCTGGTCTCAAACTCCTGGGCTCAAGTGATCCTCCTGCCTCAGCC
    TCCCAAAGTGCTGGAAGAATGAGCCACCACTCCCAGCCTGTGCCTTTACTGGAGTCTCGCTCTGTCGCCC
    ATGCTGGAGTGCAGTGACGTGAACTTGGCTCACTGTAACCTATGCCTTCCAGGTTAAACTGATTCTCCTG
    CCTCAGCCTCCTGAGTAGCCGGGACTACCGGCACCTGCCATCACACCTGGCTAATTTTTTTATTTTTAGT
    AGAGACGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAAGTGATCTGCCCGCC
    TCGGCCTCCCAAAATGCTGGGATTACAGGCGTGAACCACTGCACCCAGGCTGCCTTCTTAAAATGACTCC
    AATGTCACCTCTTAGAGAGGCATTTCCCCATCATTCACTGGTTTACTGAACTATTTCATTTTTTTATAGC
    ACCTAACATTATCTGAAATTAAAGTATTTATTGTGTCTCTCCCCTGCTAGAAGGTCAGTTTGCTCAGTAG
    CTATTTGTAGAATGAATGGATCCTCAGAAACTGCTCCTCCATCCCCTCACCCACCCCCCACTCTCGCTTC
    CCCTCTCAAACTTCTCGGACTCAGGTGGAATTCTGCTCCTGCGCTCTGCCCTAGGGTTGGGGGGGGAGCT
    GGTTGTGGAGCTGGGGGGAGGCGGAGCCTGGGCGTGGACCTGCATAGCCCAACAGGCTCTGGGGGCAGCA
    GCAAAAAGGGCGGGGGCGACAGGAACATGACAAGACGCCTCTGTGAGGCCCCGGGAGGCCCCAGCTTCTC
    AGAAAAGCCAAGAACAGAGAGTCCAGGGTGGTTAAGGGACAGAGCAGAGGAAGGGGGCCCAGGTGGTGTC
    CCCTGGCCCTTCTCCACCACTCCCCTCTGTTAAGAGGGAGAAGAGGCATGAGGGGACGTGGAAGGGAGAC
    AGACTTTTGGGCAAGGAAATAGCAAAACAGATGCGTGCAAACAGAAGACCGTCAGGAGGTGGGGGAAGGC
    TGGGGAGTAGGGGGCACTGAGAGAGAGAGGCAGGGTCTGTGGCCCCAAGAGAAGCTCAGGCCAGGCTTAA
    GGACAGAGAGCAGGGAAGCTGGAGGCCAAGGGGGTTTGCTTTGGAAGAGTCTGGGGACAGGACAGGAAGA
    GAAACAGCATCTTCTCCCCTGGGGTGGGTGCTGAATGCAAGAGTTCAAGAAGAGTTGGGGGAGGGGGGGT
    AGGAGGGGGCATCACGAGAGATGTGAGCTTGAGGCCAGGAGGGGTTTGGGGGGAGCCAGTCCTGACCGGT
    CCTGACAGGACCCAGACATCCAGTCCCAGGTTATTTTTATACTGTGGTTTGTGCCCAAGGCCAGGAAGGG
    GAAAGAGTTCGCATCCTCACACTCCCTGCCTTTCCCCAGACCTCCGAGGTGTTATATTCTCCCACAGGAC
    AGAAGTATCCAACCTTCTATCTCTGGCTGCTCACCCCCACCATGGGGACCCAGGCATCCTGCCCCCACAG
    CCTGGCTTGTTCCTCTTTTGGAGACAGGAAGGAGAAAGAGTATGGGACTGAACTCCCTTCTGGGGCTCTT
    CTCTCCCACTCCACAGTGGTCACCCTCGAGTTATCAATCACAGAGAGGCTCAGATGGACGGGGGTGAGCC
    AGACAACTGGGGGGTGAAATGGATCAACCAGCAGGCCCCCTCCCTGTCCCTGAGAGGTGTTCCCCCCACC
    CAATTTCTCAGTGACACTTGGCTTACAGCAAAGCTGGAGGCAGGACTGAGATGGGTGGCTGGGGGAAAAG
    AAAGACCCCCACATTCAGGATGGGGCTGCAGTGCAGAGACTGGCAGCGCGCAAGGCCTACCTTTTACTTT
    TCCTCCCCAAATCCTGGCAGCTCCTCCCCTAGGGACCCAGCTACCCAGCTATCCAGCTGGGGCCTCCACC
    CTCATCACCTAGAGGCAGAGGGGCTGAGACCAAGACAGAAGAAGGCCTGATGAGGGGGCCACATATGCTA
    CAGGCTACATGAAGACCAAGACAGAGAATCAGAGGCCAGGGAGAAAGAGAGAGGGTGCAGAAAAACAGGA
    AGGAGGCTGAGACAGAAAATGAAGTAGGAGGAAGGGGCTTCAGAGTGACCTGGCAGGCACAGAGAAGAGG
    GCTATCTGATCTTATAAAGTGATGGAGAAGGAGGAGGAGAGGGGGGGGAGGAGGAGGAAGGAGGCTCAAG
    ATAGAGGTTAGGCCAGGGCTGATGGGCCAGCCCTAGAACCCAGGTCTTACCTGCCCCATCTCTTCTGGAG
    CACCCAACTCCATCCGCAGCCGCATGTCTACAGCTTTCCTCCGGGACAGTGGAGGGGTCCTGGCCCCTGA
    GCCCCCAGCCTCCGCTTCCACCCTCAGAGAGGGCAGGGGCGTGGCAGCCCGGGATGTGCCCGCACTCTCA
    CCGGTCTCAGGCTCTGGGGGGCGCTGCTGGGGTGAGGAGGCCGACAGTGGGGGTTTCTCTGCCTTGGCTT
    TGCTGGGCACCACCAGCTGGCCCAGTCCCCCAGTGAAGGAGCGGGCCTGAGGTCCAGGAGAGGTGGGGGG
    TGGACGGAGCCAGGAGGTGGGGCCCTGAGACAGGCCTGACGGTGGGGTGGGGGCGCCCAGGCCGAGGCTG
    GCCAGCTCCAACCTGGAATCCAAAGATCTGCCCCCGGCGCCCCCTGAGGCCCCTCGAATTCCCTCCAGGA
    GTCCGCCGAAGCTGTGGGGCTCCAGGTCTCGCTCATAAACATCCACACACGTCCAGCGACCGCGGCGATA
    AGGCTCTCCCAGGCCGTGGGGCAGCTTCACCACCCGGAAACGGGAGGAAGGGGCCCCAGGTGGTGGGGAG
    CCATTCCGGGGGGTGCCCTTGCCCCCCGGATCGGGGCTGGGCTCCCCATTGGGCAGGCGGGGCGGGGGCC
    CGGTTGGGGGCTGTGGGGTAGGGGGATCCGAAGCCCCTGGGCTCCCAGGGCCCTCATAGTCCGTGGTGAC
    GCTGGTGATTTGGAAACTACTCTTCTTCTTGCCCCCGCTCATGGTCCCTGGGGCTCAGGGCTGGGCCAAG
    GTTGGGGGTGGGTTGGGGCTCCTTGAAGGGGCTCAGGCACCCCTGGAACAAGGGGGCCACATGGCGGGGA
    CATCCGAGCCCCTGGGGACGTCTGGGTCCGACATGGCAGGAGGGCCTGGCGGGAACCGGGGGTGCCTGCT
    GGGTGAGGGGAGAAGAGGAGAGGGGAGGTGGCGGGAGGGGGGAGCAGGGGCAGGTTTTTCCTGCTGCTGT
    TGCTGCTGAACTCCAGAGCTGAGTTTGCAAACTGGAAAAAGAGGGGGAGAGAGAGAGAGAGAGAGAGAGA
    GAGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAAACCAGTGGGCAGG
    CCACCTCCGGGGCTTCCTCTCCGGGGAGCAGAGTGGGGGAAGGGGAAACCGTCAGAGACCCAGAGACAGG
    CAAGAGATGACAGAGACCAGGGCAGAGGGGACTGGATCCAAGAAGGCACTCAGAGTAGAAAGAGGCAGAT
    GAGTCCCAGAGTTGGGCATTTCTCGCCTGGGATTCTGACTCAGGGGGCCCGTATTGTCCCAAGAGCTCGG
    ACTCCAGGGCCTGGGAGAACCAGGCTGAAGCAGCTCCCCAGCCTGGCCCGGCCACTCAGAGAGACCTCTC
    CTGGGCCTCGAGCCCCCACCCTCACCCCAGCTCCCACATCTCTGGGGCAGGCCCTGCCCGGCTACCCCCC
    AGCGCCCCACCCAGCCCTGAACCCTACCTCTGGGGCCTCGGCCACTGATCTGAGCCTGAGGGTCCCGGCG
    CCTAAGAGGTCGGGCTGTCCGTTCCCTGGGGCCTCCTGGCCATGGGCAGTGGCGGGGCACGCAGGCGGCG
    ATCAGAGGCTGTCCCGTCCTCTCCGGGGGCCGCGGCTCATCCTGCCAGGCATCTCCGAGGAAAGTTTGCT
    CTCCGGAAAAGAAGAAACCCGCGCCTCCTCCGAGGAGCCTAGGAGCGGCCTCTGAAGCCCCAAGGGTCTG
    GAGACTGATGTGGGAGGGGGCGGCCGAGCCCCCCTCTGCTCCCGGTGGCAGCCAGCGAGTGGGTGTCCCG
    AGTCTCCTCCCGCGTGACCCTGCTGGGTCCGTGTCTCCGCTCCTCGGCTCCCCTGGGTCTCTGCCTCTCC
    GTC
  • Protein Sequence : Show Sequence
    >gi|13569901|ref|NP_112197.1| TSC22 domain family protein 4 [Homo sapiens]
    MSGGKKKSSFQITSVTTDYEGPGSPGASDPPTPQPPTGPPPRLPNGEPSPDPGGKGTPRNGSPPPGAPSS
    RFRVVKLPHGLGEPYRRGRWTCVDVYERDLEPHSFGGLLEGIRGASGGAGGRSLDSRLELASLGLGAPTP
    PSGLSQGPTSWLRPPPTSPGPQARSFTGGLGQLVVPSKAKAEKPPLSASSPQQRPPEPETGESAGTSRAA
    TPLPSLRVEAEAGGSGARTPPLSRRKAVDMRLRMELGAPEEMGQVPPLDSRPSSPALYFTHDASLVHKSP
    DPFGAVAAQKFSLAHSMLAISGHLDSDDDSGSGSLVGIDNKIEQAMDLVKSHLMFAVREEVEVLKEQIRE
    LAERNAALEQENGLLRALASPEQLAQLPSSGVPRLGPPAPNGPSV
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine
IV. Vaccine Information
1. Ag85B and ESAT-6 fusion protein
a. Vaccine Ontology ID:
VO_0000536
b. Type:
Conjugate vaccine
c. Antigen
Two immunodominant antigens, Ag85B and ESAT-6, were used in the analysis. ESAT-6 is a 6-kDA early secretory antigenic target. The two antigens are combined as a fusion protein in the vaccine (Olsen et al., 2001).
d. Gene Engineering of EsxA (ESAT-6)
  • Type: Fusion protein ESAT6-Ag85B
  • Description: Fusion protein created in E. coli using His-tag cloning system pMCT6. Proteins were affinity purified, separated via ion exchange chromatography, and analyzed with SDS-PAGE and Western blots (Olsen et al., 2001).
  • Detailed Gene Information: Click here.
e. Gene Engineering of Ag85B from M. tuberculosis H37Rv
  • Type: Fusion protein Ag85B-ESAT6
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant: MPL vaccine adjuvant
  • VO ID: VO_0001250
  • Description: 1 25 μg amount of monoposphoryl lipid A (MPL) adjuvant was added to 250μg dimethyl dioctadecylammonium bromide (DDA) emulsifier per 0.01-50μg of vaccine.
g. Adjuvant: DDA vaccine adjuvant
h. Preparation
M tuberculosis Erdamn and H37Rv were grown in media, while BCG Danish 1331 was acquired as a freeze dried-vaccine and replenished (Olsen et al., 2001). Recombinant ESAT-6 was obtained along with short-term culture filtrate. Esat6 and ag85B coding regions were obtained by PCR amplification from M. tuberculosis H37Rv chromosomal DNA using selected primers. PCR products were digested by two restriction endonucleases and cloned into pMCT6 prior to sequencing. The E. coli XL-1 Blue was used to express the His-tagged protein prior to purification, protein anion-exchange chromatography, and dialysis. PCR fragments per gene were joined at a HindIII site, cloned into pMCT6, expressed, and later purified to create a fusion protein and a chimeric plasmid.

i. Mouse Response
  • Host Strain: C57BL/6J (H-2^b), specific pathogen-free female; B6CBAF1
  • Vaccination Protocol: Female mice 6-12 weeks old were housed within a BSL-3 enclosure. Mice were immunized with 0.01-50 μg vaccine emulsed in 250 μg dimethyl dioctadecylammonium bromide (DDA) and 25 μg of monophosphoryl lipid A (MPL) aduvant. Vaccines were injected 3 times subcutaneously on back at 2-week intervals (Olsen et al., 2001). BCG Danish 1331 dose (5x104 baccilli/mouse) was administered via subcutaneous injection at base of tail simultaneously with first subunit vaccination. No booster injections were included (Olsen et al., 2001).
  • Persistence: Mice were sacrificed at either 2 or 6 weeks post-innoculation via i.v. or aerosol routes, respectively prior to organ removal and bacterial enumeration(Olsen et al., 2001).
  • Challenge Protocol: Prechallenge immunity was assessed 5 weeks post-first vaccination (Olsen et al., 2001).
    Mice were challenged at either 10 or 30 weeks post-immunization via aerosol route (100 CFU of M. tuberculosis Erdman per lung) or intravenous route (0.2 ml volume containing 5x104 CFU M. tuberculosis H37Rv in PBS) (Olsen et al., 2001).
  • Efficacy: A statistical reduction in the number of bacteria was observed for vaccine doses as low as .01μg. The 0.1-10μg dose range showed a higher level of protection versus the .01μg dose. 50 μg doses resulted in reduced protection in lung and spleen, whereas, 10 μg showed significant levels of protection in spleen. The fusion protein Ag85B-ESAT-6 showed a statistically significant improvement in reduction of bacteria versus the individual Ag85B and ESAT-6 components (Olsen et al., 2001). Both recombinant proteins induced long-lived memory immunity when challenged 10 or 30 weeks post-first vaccination, while BCG showed significantly lower protection levels at the later challenge points(Olsen et al., 2001).
  • Description: Blood lymphocytes were purified and pooled from eight mice per group and cultured in triplicate as 2x105 cells in 200μl of RPMI 1640 with 2-mercaptoethanol, glutamine, penicillin-streptomycin, and 5% v/v fetal calf serum. Mycobacterial antigens administered as 5-1.3μg/ml. Interferon gamma amounts were determined using ELISA (Olsen et al., 2001).
2. Arabinomannan-tetanus toxoid conjugate
a. Vaccine Ontology ID:
VO_0000528
b. Type:
Conjugate vaccine
c. Antigen
Arabinomannan (AM) oligosaccharides. They are derived from Lipoarabinomannan (LAM), a carbohydrate antigen expressed on the surface of mycobacteria which contains a manna polysaccharide core attached to a phosphatidyl inositol lipid moiety (Hamasur et al., 2003). The surface carbohydrate was hypothesized to yield CD1-specific immune responses, promote mycobacterial clearance, downregulate T cell proliferation, and interfere with activation of macrophages through interferon gamma intervention (Hamasur et al., 2003).
d. Gene Engineering of Ag85B from M. tuberculosis H37Rv
  • Type: Conjugate protein purified from LAM
  • Description: The 28-kDa AM oligos were pooled and linked to tetanus toxoid (TT) or other M. tuberculosis proteins (Ag85B, 75-kDa protein) with thioether linkage generated by conjugation method. Conjugation consisted of amination of AMO with ammonium chloride and sodium cyanoborohydride, deasalting and treatment with 2-imminothiolane, and end conjugation between thiolated oligsaccharide derivatives with bromoacetylated proteins and tributyl phosphine (Hamasur et al., 2003).
  • Detailed Gene Information: Click here.
e. Adjuvant: aluminum hydroxide vaccine adjuvant
  • VO ID: VO_0000127
  • Description: Alum (aluminum hydroxide gel) adjuvant was used initially. Eurocine L3 suspension adjuvant was prepared using 1:1 ratio solid monooleate and oleic acid, Tris buffer with pH adjustment including NaOH, and subsequent sonification. Eurocine L3 emulsion was prepared using 1:1 ratio solid monooleate and oleic acid which were liquified prior to addition of soy bean oil (Hamasur et al., 2003).
f. Preparation
Arabinomannan (AM) oligosaccharides derived from LAM of Mycobacterium tuberculosis H37Rv were isolated and covalently conjugated to tetanus toxoid (TT) or to short-term culture filtrate proteins (antigen 85B (Ag85B) or a 75kDa protein) from M. tuberculosis strain Harlingen (Hamasur et al., 2003).
g. Mouse Response
  • Host Strain: C57BL/6, 8-10 wk female
  • Vaccination Protocol: Mice were injected subcutaneously in flanks with 100μL or intranasal with 5μL/nostril of antigen plus adjuvant. The control BCG vaccination involved subcutaneous injection of 5x105 CFU in 100μL PBS in hind flank. Two formulations of the adjuvant were used, such that mice were either treated at day 0 with AMOs-TT conjugate in Eurocine L3 emulsion or suspension, followed by nasal booster at day 21 (Hamasur et al., 2003).
  • Immune Response: Immunization with AMO-TT, AMOs-Ag85B, or AMOs-75kDa conjugate resulted in increased lymphocyte proliferation in spleen when responding to PPD (Hamasur et al., 2003).
  • Challenge Protocol: Mice were challenged intranasally with 105 M. tuberculosis Harlingen on day 24 (vaccination on day 0).
  • Efficacy: All mice started to die after 17 weeks (median time = 36 weeks) (Hamasur et al., 2003). Considerable increases in weight were observed for mice immunized with AMOs-TT conjugate or BCG versus those not vaccinated (Hamasur et al., 2003). Mice immunized with AMOs-TT conjugate and either adjuvant showed longer survival than sham-immunized mice. The emulsion adjuvant showed increased protective efficacy versus the suspension version (Hamasur et al., 2003)
h. Mouse Response
  • Host Strain: C57BL/6, 8-10 wk female
  • Vaccination Protocol: Mice were immunized subcutaneously on days 0, 28 with 5 or 25 μg of AMO-Ag85B in 1% alum. Control mice were vaccinated s.c. with live BCG on day 0.
  • Side Effects: Reduced weight loss observed for BCG and AMO-Ag85B immunized mice versus shams (Hamasur et al., 2003).
  • Challenge Protocol: Mice were challenged intravenously with 105 CFU M. tuberculosis H37Rv on day 60(Hamasur et al., 2003).
  • Efficacy: BCG-immunized mice exhibited best survival versus mice immunized with the AMO-Ag85B vaccine. However, AMO-Ag85B immunized mice also showed significant improvement in survival versus sham-immunized mice.
i. Guinea pig Response
  • Host Strain: Dunkin Hartley, female (250-300g)
  • Vaccination Protocol: Guinea pigs were immunized s.c. (day 0) and boosted nasally (day 24) with Eurocine L3 emulsion, AMO-TT conjugate, and variable antigen doses and adjuvant concentrations (Hamasur et al., 2003). A protection study included immunization with 15 μg AMO-Ag85B in 10% Eurocine L3 emulsion. Comparison BCG groups were injected s.c. with 5x104 CFU live BCG (Hamasur et al., 2003).
  • Side Effects: Five of six animals non-vaccinated and two of six AMO-Ag85B vaccinated were killed before 120 days post-challenge if observed >20% loss of starting weight.
  • Challenge Protocol: Guinea pigs were aerosol challenged with saline suspension containing 106 organisms per ml (~10 CFU/lung).
  • Efficacy: BCG vaccinated animals showed reduced counts of M. tuberculosis bacilli in lung and spleen samples versus saline controls. The reduced CFUs in spleen samples along with histological sections analyzed with double blind analysis suggested an overall decrease in disease severity in lung and spleen (Hamasur et al., 2003).
j. Rabbit Response
  • Host Strain: White New Zealand female rabbits (2.5-3kg)
  • Vaccination Protocol: Rabbits were immunized with LAM (50 μg), AMO-TT (20 μg), AMO-Ag85B (20 μg), or AMO-75kDA (20 μg) conjugates via i.m. injection with 100 μl PBS and 100 μl FIA emulsifier, and later boosted (weeks 2, 4) with same dose of the previous vaccine.
  • Immune Response: LAM alone did not induce LAM IgG antibodies, whereas the three conjugate vaccines induced high IgG antibody titers (Hamasur et al., 2003).
  • Challenge Protocol: No challenge protocol implemented for rabbit immunogenicity study.
  • Description: Purpose of the experiment with rabbits was to determine immunogenicity response to LAM and AMO-protein conjugates.
3. attenuated M. bovis strain WAg539
a. Vaccine Ontology ID:
VO_0000543
b. Type:
Live, attenuated vaccine
c. Antigen
M. bovis with gene mutations due to signature-tagged mutigenesis to create attenuated mutants. Ten genes were found to be deleted or interrupted in the 10.1-kb deletion in WAg539, including mmpL4, mmpS4, ufaA1, and sigK, as well as unknown genes (Collins et al., 2005).
d. Preparation
M. bovis strain and its recombinants were cultured in Middlebrook 7H9 (Difco) liquid medium supplemented with albumin-dextrose complex (Difco), 0.05% Tween 80, and and 0.4% sodium pyruvate and on Middlebrook 7H11 (Difco) solid medium supplemented with albumin-dextrose complex and 0.4% sodium pyruvate. Recombinants were cultured on kanamycin-supplemented medium. M. bovis culture from pig spleens were homogenized, filtered, and further processed (Collins et al., 2005). Forty-five recombinants with unique tags were subcultured separately and pooled together. The forty-five M. bovis recombinants were subcultured separately, and 106 CFU of each pool was inoculated subcutaneously into the flank of each guinea pig. Splenic culture were extracted from sacrificed animals and used to isolate 500-2,000 colonies per spleen. Tags identified in both the inoculated and recovered pools were amplified by PCR and processed.
e. Description
Signature tag morphogenesis was used to generate illegitimate recombinants of M. bovis. Several of the recombinant strains produced protection that was "at least as good as that provided by M. bovis BCG" in guinea pig models (Collins et al., 2005).
f. Guinea pig Response
  • Host Strain: Dunkin-Hartley
  • Vaccination Protocol: Groups of six guinea pigs were vaccinated with 105 CFU of one of the avirulent recombinant strains or BCG. Six animals were not vaccinated (control group) (Collins et al., 2005).
  • Challenge Protocol: Guinea pigs were challenged by aerosol with 2 to 10 CFU of wild-type M. bovis eight weeks post-vaccination (Collins et al., 2005).
  • Efficacy: Four mutants which did not induce any visible lesion were tested for vaccine efficacy. In the case of three mutants, animals were sacrificed 5 weeks post-challenge. The final mutant, WAg585 were sacrificed at 8 weeks post-challenge. Mutant WAg539 gave better, though not significantly different, protection than BCG vaccine, as well as the best vaccination of the four mutants (Collins et al., 2005).
  • Description: Animals were sacrificed and autopsied 5 weeks after challenge to enable the following analyses: weight, gross pathology, mycobacterial culturing and enumeration in splenic and lung tissues, and statistical analysis of macroscopic lesion count and other quantifiable measurements (Collins et al., 2005).
4. Bacille Calmette-Guérin (BCG)
a. Tradename:
BCG
b. Vaccine Ontology ID:
VO_0000771
c. CDC CVX code:
19
d. Type:
Attenuated live vaccine
e. Preparation
The evaluation of BCG lacks comparability among the vaccination studies due to the use of six different BCG sub-strains [Copenhagen-1331, Pasteur-1173P, Glaxo-1077, Tokyo-172, Russian, Moreau]. The comparative genomic analysis of different BCG strains has shown that there is no uniformity among all presently used vaccines and this genetic variability may contribute to the variation in protective efficacy seen in different geographic regions of the world (Gupta et al., 2007).

In one case, Calmette et al. (1923) used decreasing doses (1 mg, .01 mg, and .002 mg) of tubercle bacilli to infect guinea pigs s.c. Bacterial loads increased in response to decreasing dose sizes. Similar results were acquired for later intramuscular and intracerebral infection procedures by other groups (SWEDBERG, 1951)
f. Virulence
The original BCG strain lost its virulence after 39 passages by Albert Calmette and Camille Guerin, and was administered to humans i.d. on the 231st passage. The BCG bacteria showed altered colony morphologies and no virulence in experimental animals. BCG has shown a high degree of safety over the years (Ducati et al., 2006).

--Calmette et al. used length of survival as a metric for virulence in their 1923 study (Agger et al., 2002).
--The increased ability for M. bovis strains to retain virulence was noted by Lange in 1922 (SWEDBERG, 1951).
g. Description
Albert Calmette and Camille Guerin, two French scientists, made an attenuated strain from another mycobacterium (M. bovis) by growing it for 13 years on culture. They observed a reduction in the virulence in animals through this period. When infants were given this vaccine, it provided a reduction in mortality by 90%. Since then, Bacille Calmette Guerin [BCG] has become the most widely used vaccine throughout the world. It is estimated that more than 3 billion people have received BCG. The protective efficacy of BCG vaccine against adult pulmonary TB has varied dramatically from 80 to 0% (Gupta et al., 2007).

Development of the BCG strain was predated by numerous M. tuberculosis studies from the 1890s-1920s, including (Lote 1889, Strauss 1895, Moriya 1909, Romer 1903, Weber 1912, Binder 1915, and Browning and Gulbransen 1926) in a diverse set of hosts (including mouse, avian, human, bovine, and guinea pig hosts) (SWEDBERG, 1951).

Several new subunit booster vaccinations are under development, including Ag85, Mtb72F, HspX and other DosR-controlled gene products, Rv3407 and other Rpf-controlled gene products, and heparin-binding hemagglutinin (HBHA) (Kaufmann, 2005).
h. Human Response
  • Immune Response: TB Ag-specific mucosal, humoral and cellular immune response via T cell proliferation and production (Wang et al., 2009).
  • Description: SL(E6-85B) vaccine, when combined with BCG vaccination, induced the strongest TB Ag-specific mucosal, humoral, and cellular immune responses comprised of increased proliferation of T cells, IFN-gamma expression, granzyme B production (Wang et al., 2009).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced significant TB Ag-specific IFN-gamma immune responses in splenocytes. Splenocytes were stimulated with the Ag85B protein 2 weeks after the final immunization (Wang et al., 2009).
    • Detailed Gene Information: Click here.
i. Human Response
  • Vaccination Protocol: DNA was obtained from the peripheral blood of 99 NMIBC patients who were prospectively randomized to receive postresection intravesical BCG or BCG with interferon alpha. (Chiong et al., 2011)
  • Immune Response: Patients with the NRAMP1 D543N G:G genotype and allele 3 (GT)n polymorphism had decreased recurrence time after BCG therapy. (Chiong et al., 2011)
  • Host Gene Response of GPX1
    • Gene Response: Polymorphisms of the GPX1 gene may be associated with recurrence of BCa after BCG immunotherapy. (Chiong et al., 2011)
    • Detailed Gene Information: Click here.
  • Host Gene Response of SLC11A1
    • Gene Response: Polymorphisms of the SLC11A1 genes may be associated with recurrence of BCa after BCG immunotherapy. (Chiong et al., 2011)
    • Detailed Gene Information: Click here.
j. Human Response
  • Vaccination Protocol: Intradermally vaccinated the infants within 48 hours of birth. Examination of whole blood stimulated ex vivo with 1.2×106 CFU BCG 10 weeks after newborn BCG vaccination of South African infants (Randhawa et al., 2011).
  • Immune Response: Increased production of TH1-type T cell cytokines (Randhawa et al., 2011).
  • Host Gene Response of GPX1
    • Gene Response: After stimulation with TLR6 lipopeptide ligands, PBMCs from TLR6-deficient, BCG-vaccinated individuals secreted lower amounts of IL-6 and IL-10 compared to those with responsive TLR6 genotypes (Randhawa et al., 2011).
    • Detailed Gene Information: Click here.
  • Host Gene Response of human IFNG
    • Gene Response: Increased IFNG production associated with TLR1/6 presence 10 weeks post BCG vaccination (Randhawa et al., 2011).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL10
  • Host Gene Response of IL2
    • Gene Response: Increased IL2 production associated with TLR1/6 presence 10 weeks post BCG vaccination (Randhawa et al., 2011).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL6
    • Gene Response: IL6 expression post vaccination expressed in higher amounts in presence of TLR1/6 (Randhawa et al., 2011).
    • Detailed Gene Information: Click here.
  • Host Gene Response of TLR1
    • Gene Response: After stimulation with TLR1 lipopeptide ligands, PBMCs from TLR1-deficient, BCG-vaccinated individuals secreted lower amounts of IL-6 and IL-10 compared to those with responsive TLR1 genotypes (Randhawa et al., 2011).
    • Detailed Gene Information: Click here.
k. Mouse Response
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: Immunization with BCG[pMV361] (BCG with empty pMV361) vaccine induced significant up regulation of IFN-gamma in mice 45 days after immunization as compared to non-immunized mice. Cytokines were measured in lymphocytes from the spleen (Singh et al., 2011).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-6
    • Gene Response: Immunization with BCG[pMV361] (BCG with empty pMV361) vaccine induced significant up regulation of IL-6 in mice 45 days after immunization as compared to non-immunized mice. Cytokines were measured in lymphocytes from the spleen (Singh et al., 2011).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Il2
    • Gene Response: Immunization with BCG[pMV361] (BCG with empty pMV361) vaccine induced significant up regulation of IL-2 in mice 45 days after immunization as compared to non-immunized mice. Cytokines were measured in lymphocytes from the spleen (Singh et al., 2011).
    • Detailed Gene Information: Click here.
  • Host Gene Response of TNF-alpha
    • Gene Response: Immunization with BCG[pMV361] (BCG with empty pMV361) vaccine induced significant up regulation of TNF-alpha in mice 45 days after immunization as compared to non-immunized mice. Cytokines were measured in lymphocytes from the spleen (Singh et al., 2011).
    • Detailed Gene Information: Click here.
l. Mouse Response
  • Host Strain: C57BL/6
  • Vaccination Protocol: Ten to twelve female C57BL/6 mice were immunized per group with 100 μl of the formulation, once, subcutaneously (s.c.) at the base of the tail. BCG and Lactoferrin were emulsified with Freund's adjuvant in a 1:1 ratio (Hwang et al., 2005).
  • Immune Response: A single immunization of mice with Lactoferrin as an adjunct adjuvant resulted in amplified splenocyte proliferative response to heat-killed BCG, and elevated IL-12(p40) production with increased relative ratios of IL-12/IL-10. Furthermore, splenocyte recall response to HK-BCG was augmented for proinflammatory mediators, TNF-alpha, IL-1beta, and IL-6, approaching responses generated to complete Freund's adjuvant (CFA) immunized controls (Hwang et al., 2005).
  • Challenge Protocol: Fourteen days post-immunization, four mice from each group were aerosol challenged with Erdman MTB (Hwang et al., 2005).
  • Efficacy: All immunization groups showed significant reduction in lung organism load, decreased bacterial load in the spleen (Hwang et al., 2005).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: Lactoferrin was able to significantly augment the production of IFN-γ compared to vaccine preparations in IFA alone (Hwang et al., 2005).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-1b
    • Gene Response: In concert with the increased stimulation index, there was significant production of all three proinflammatory mediators (TNF-alpha, IL-1beta, and IL-6) in the Lactoferrin immunization group compared to both the non-immunized and IFA immunized groups (Hwang et al., 2005).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-6
    • Gene Response: In concert with the increased stimulation index, there was significant production of all three proinflammatory mediators (TNF-alpha, IL-1beta, and IL-6) in the Lactoferrin immunization group compared to both the non-immunized and IFA immunized groups (Hwang et al., 2005).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Il12b
    • Gene Response: A single administration of BCG in IFA and Lactoferrin resulted in significantly increased production of IL-12 in the splenic recall assay (Hwang et al., 2005).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ltf
    • Gene Response: Lactoferrin can act as an adjunct adjuvant to augment cellular immunity and boost BCG efficacy for protection against subsequent challenge with virulent MTB. Augmented responses were found for IL-12, TNF-alpha, IL-1beta, and IL-6 (Hwang et al., 2005).
    • Detailed Gene Information: Click here.
  • Host Gene Response of TNF-alpha
    • Gene Response: In concert with the increased stimulation index, there was significant production of all three proinflammatory mediators (TNF-alpha, IL-1beta, and IL-6) in the Lactoferrin immunization group compared to both the non-immunized and IFA immunized groups (Hwang et al., 2005).
    • Detailed Gene Information: Click here.
m. Guinea pig Response
  • Host Strain: Dunkin Hartley (Thom et al., 2012).
  • Vaccination Protocol: Pathogen-free, female outbred Dunkin Hartley strain guinea pigs (weighing 500–550 g) were vaccinated subcutaneously with 5 × 104 colony forming units (CFU) of M. bovis BCG Pasteur (Thom et al., 2012).
  • Immune Response: Significant down-regulation of both ferritin light- and heavy-chain (Thom et al., 2012).
  • Description: PPD from M. tuberculosis was added to splenocytes from BCG-vaccinated guinea pigs at a final concentration of 30 μg/ml for 16 and 24 h at 37 °C in the presence of 5% CO2. The splenocyte cultures from naïve and BCG-vaccinated guinea pigs were infected with M. tuberculosis at an MOI of 0.2 and incubated for 4, 16 and 24 h at 37 °C in the presence of 5% CO2. The viability of uninfected and infected splenocytes from the same vaccination group was determined at 4, 16 and 24 h post-M. tuberculosis infection by trypan blue exclusion (Thom et al., 2012).
  • Host Gene Response of Fth1
    • Gene Response: Splenocytes from BCG-vaccinated guinea pigs were stimulated ex vivo with purified protein derivative from M. tuberculosis and a significant down-regulation of ferritin light- and heavy-chain was measured by reverse-transcription quantitative-PCR (Thom et al., 2012).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ftl
    • Gene Response: Splenocytes from BCG-vaccinated guinea pigs were stimulated ex vivo with purified protein derivative from M. tuberculosis and a significant down-regulation of ferritin light- and heavy-chain was measured by reverse-transcription quantitative-PCR (Thom et al., 2012).
    • Detailed Gene Information: Click here.
n. Rat Response
  • Host Strain: Wistar rat
  • Vaccination Protocol: Wistar rats were vaccinated with 106 BCG subcutaneously 6-8 weeks prior to challenge (Singhal et al., 2011).
  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: A significantly greater number of total cells and CD8 T cells was observed in BCG vaccinated animals at day 60 post-challenge compared with unvaccinated animals. Immunohistochemistry revealed substantial higher number of CB8 cells in the lung lesions of vaccinated rats at day 30. Finally, a high number of mycobacteria-specific CD4 and CD8 T cells producing IFN-gamma was detected in BCG vaccinated rats (Singhal et al., 2011).
  • Challenge Protocol: The rats were challenged with a low dose of Mtb W4 strain 6 weeks after vaccination (Singhal et al., 2011).
  • Efficacy: A significantly reduced bacillary load was observed in the lungs of BCG vaccinated rats over the entire course of infection. About a 1 log(10) reduced bacillary load was observed in the lungs of vaccinated rats compared to unvaccinated animals (Singhal et al., 2011).
  • Description: The study showed that BCG vaccinated Wistar rats efficiently control early bacillary growth and pathology related to Mtb infection (Singhal et al., 2011).
o. Cattle Response
  • Host Strain: Friesian Calves
  • Vaccination Protocol: Vaccinated subcutaneously in the neck with 2 mL of 2X105 cfu BCG
  • Persistence: Cattle were examined 154 to 164 days after they had been challenged.
  • Vaccine Immune Response Type: VO_0000408
  • Immune Response: The cattle that had been vaccinated with BCG showed significantly less numbers of lesions in the lungs and lymph nodes.
  • Challenge Protocol: 54 days after vaccination the calves were challenged intratracheally with 2X103 cfu M. bovis strain 83/6235.
  • Efficacy: Vaccination with BCG induced considerable protection against a challenge with M. bovis, there were significantly fewer animals with Tuberculosis lesions.
  • Description: This study showed that killed M. vaccae did not protect against challenge by M. bovis, however, the vaccination of cattle with BCG by the respiratory route protected them against the development of tuberculous lung lesions.
  • Host Gene Response of IFNG
    • Gene Response: In the vaccinated cattle group, increased IFN-gamma was released from the culture of peripheral blood lymphocytes from cattle after stimulation with bovine purified protein derivative (PPD) (Buddle et al., 1995).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL10
    • Gene Response: In the vaccinated cattle group, increased IL-2 was released from the culture of peripheral blood lymphocytes from cattle after stimulation with bovine purified protein derivative (PPD) (Buddle et al., 1995).
    • Detailed Gene Information: Click here.
p. Macaque Response
  • Host Strain: cynomolgus monkey (M. fascicularis) and rhesus monkey (M. mulatta)
  • Vaccination Protocol: The macaques were vaccinated intradermally with 1–4 X 105 cfu bacillus Calmette–Guerin
  • Vaccine Immune Response Type: VO_0000859
  • Immune Response: Showed specific cell-mediated immune responses after bacillus Calmette–Guerin vaccination as observed by skin test, lymphoproliferation, and IFN-gamma production (Langermans et al., 2001).
  • Challenge Protocol: 17 weeks after vaccination the monkeys were challenged with 3,000 cfu M. tuberculosis by intratracheal installation of 3 ml of the bacterial suspension. Between 59 and 65 days after infection the animals were killed, and necropsies were undertaken for pathological examination (Langermans et al., 2001).
  • Description: This study showed that cynomolgus monkey (M. fascicularis) is a better model than rhesus monkey (M. mulatta) for M. tuberculosis vaccination and infection studies. From a challenge with 3,000 cfu M. tuberculosis, BCG vaccination protects cynomolgus monkeys with >2-log reduction of the bacterial load and diminished pathology, whereas only a minimal BCG vaccination effect was observed in rhesus monkeys. These two species represent the two extremes of BCG-induced protection that is found in humans (Langermans et al., 2001).
q. Rabbit Response
  • Host Strain: New Zealand White Rabbits
  • Vaccination Protocol: Rabbits were vaccinated three times at 3-week intervals intramuscularly with a total of 500 micro liters of BCG (Tsenova et al., 2006).
  • Persistence: Rabbits were euthanized 8 weeks post-infection (Tsenova et al., 2006).
  • Vaccine Immune Response Type: VO_0000134
  • Immune Response: Significantly smaller amounts of CFU of the bacterium were observed in the brains of rabbits that were vaccinated with BCG. No granulomas were found in the lungs of mice vaccinated with BCG while they were found in the unvaccinated group. Vaccination with BCG induced a statistically significant increase in antigen-specific T-cell proliferation compared to that of non-vaccinated controls (Tsenova et al., 2006).
  • Challenge Protocol: Ten weeks after immunization with BCG the rabbits were anesthetized and immobilized and a spinal needle was used to withdraw .3 mL of cerebrospinal fluid and .2 mL of 5X105 CFU of M. tuberculosis was injected intracisternally (Tsenova et al., 2006).
  • Efficacy: This study showed that vaccination with BCG induced protection against M. tuberculosis because rabbits that were vaccinated showed increased T-cell proliferation and no granulomas in the lungs (Tsenova et al., 2006).
r. Zebrafish Response
  • Host Strain: Wildtype AB or adult rag hu1999 mutant fish
  • Vaccination Protocol: Fish were vaccinated with ~8X103 CFU BCG intraperitoneally or intramuscularly (Oksanen et al., 2013).
  • Vaccine Immune Response Type: VO_0003057
  • Challenge Protocol: Fish were anesthetized and intraperitoneally injected 3-4 weeks after immunization with ~20-30 bacteria (Oksanen et al., 2013).
  • Efficacy: BCG was unable to prevent infection with mycobacteria, however, it improved fish survival lowering the mortality rate from 84.2% to 45.0% (Oksanen et al., 2013).
  • Description: BCG is unable to prevent infection in zebrafish, however, it is able to improve fish survival (Oksanen et al., 2013).
5. BCG auxotroph expressing HIV-1 clade immunogen
a. Vaccine Ontology ID:
VO_0004122
b. Type:
Live, attenuated vaccine
c. Antigen
HIVA, an African HIV-1-derived immunogen, was identified as stable and inductive of HIV-1-specific CD4+ and CD8+ T cell responses. HIVA in this study contained an immunodominant H-2D^d restricted epitope P18-I10 (Im et al., 2007).
d. Preparation
HIVA gene with mAb tag Pk was fused to M. tuberculosis 19-kDa lipoprotein signal sequence via PCR to improve foreign protein immunogenicity. HIVA was cloned into two plasmids, pJH222 and pJH223, as HindIII-HindIII fragment and used to transform a lysine auxotroph of BCG (BCG Pasteur lysA5::res). Additional preparations included MVA.HIVA and BCG.p. BCG cultures were grown in presence of kanamycin. HIVA immunogenicity was assessed using H-2D^d-restricted epitope P18-I10 and subdominant H-2K^d-restricted P epitope. MHC class II-restricted responses were tested using three epitope peptides derived from p24 (Im et al., 2007).
e. Description
Goal of study was to develop a vaccine to minimize the risk of mother-to-child transmission (MTCT) of M. tuberculosis and HIV-1, and to protect against both pathogens in newborns. BCG was selected as a vaccine vector due to its long efficacy as a vaccine (10-15 years), frequent use in infant and newborn vaccination, and induction of humoral and cellular responses (Im et al., 2007).
f. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were immunized on day 0 with either BCG.HIVA (pJH222.HIVA) and episomal plasmid, parental BCG (BCG.p), or control (no immunization). Half of the animals were boosted with MVA.HIVA on day 102. Mice were sacrificed on day 151. T cells were stimulated with peptides in a multicolor flow cytometric analysis to elicite interferon gamma, tumor necrosis factor alpha, and degranulating responses (Im et al., 2007).
  • Immune Response: HIVA-specific responses peaked after 12 weeks, most likely as a result of BCG-induced activation of T cells. Three major observations were observed:
    1) BCG.HIVA alone induces undetectable CD8+ HIV-1-specific T cell responses secreting tum, though priming with BCG.HIVA shows significant increases in elicited H and P-specific splenocytes producing IFN-gamma and degranulating. Hence, BCG.HIVA enhancement of MVA.HIVA responses is HIVA-specific.
    2) BCG.HIVA priming with MVA.HIVA boost elicits the highest proportion of HIV-1 specific bifunctional cells in all the studies.
    3) No HIVA-induced CD4+ T cell responses were detected (Im et al., 2007).
g. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were primed with increasing doses (103-10^7 CFU/animal, day 0) of BCG.HIVA or not vaccinated, and boosted (day 102) with constant doses of MVA.HIVA.
  • Immune Response: Bifunctional responses increased steadily with dose up to 0.7% CD8+ splenocytes with 106 CFU BCG.HIVA for epitope H , and increased up to 0.2% CD8+ with 107 CFU for P response (Im et al., 2007). Priming dose of BCG.HIVA affects strength and quality of CD8+ T cell responses induced by the recombinant BCG priming followed by recombinant MVA boosting, with high priming dose being most important for quality of response to P epitope (Im et al., 2007).
h. Mouse Response
  • Host Strain: BALB/c, 6-8 wk old
  • Vaccination Protocol: Pathogen free mice were vaccinated in groups of 7-9 animals with 3x105 CFU of BCG.HIVA, BCG.p, or BCG wild type 1173 p2 via s.c. injection into left hind foot.
  • Challenge Protocol: Mice were challenged via aerosol (nasal) route 12 weeks post-vaccination with M. tuberculosis Erdman using a modified Henderson apparatus. Approximately 25 CFU/lung were estimated 24 hours post-challenge (Im et al., 2007).
  • Efficacy: All three vaccines tested here showed an approximate 2-fold reduction in M. tuberculosis load in the lungs and spleen. Data suggest that the "safer" lysine auxotrophBCG.HIVA could replace BCG vaccine in neonatal uses and still show comparable efficacy (Im et al., 2007).
i. Mouse Response
  • Host Strain: BALB/c, female
  • Vaccination Protocol: Groups of 4-5 mice were naive or immunized with 100 μg pTHr.HIVA, 106 pfu MVA.HIVA, or 106 cfu of either BCG.p or BCG.HIVA. BCG.HIVA boosting commenced on day 33.
  • Immune Response: No HIVA-specific responses were noticed during the 4-day WR.HIVA infection. Mice receiving the pTHr.HIVA-BCG.p, pTHr.HIVA-BCG.HIVA , or BCG.HIVA showed both H and P specific responses. Highest responses were seen in pTHr.HIVA-BCG.HIVA-WR.HIVA which showed interferon gamma and degranulated responses. CD4+IFN-gamma+TNF-alpha+IL-2+ splenocytes exhibited high quality characteristics in the BCG.HIVA and BCG.p boosts following pTHr.HIVA priming. HIVA non-specific mechanisms were suggested by a two-log10 decrease in WR.HIVA titre when BCG.HIVA or BCG.p alone were used in immunization (Im et al., 2007).
  • Challenge Protocol: Mice were challenged i.p. using 4x106 pfu vaccinia virus WR.HIVA. Plaque counts were determined for sonicated and serially-diluted mouse ovaries four days post-challenge (Im et al., 2007).
  • Description: Surrogate challenge with recombinant replication-competent vaccinia virus (strain Western Reserve) is a cost-effective murine alternative to HIV-1challenge in non-human primate models.
6. BCG mutant secreting listeriolysin
a. Vaccine Ontology ID:
VO_0000603
b. Type:
Subunit vaccine
c. Antigen
The tuberculosis vaccine Mycobacterium bovis bacille Calmette-Guérin (BCG) was equipped with the membrane-perforating listeriolysin (Hly) of Listeria monocytogenes (Grode et al., 2005).
d. Preparation
BCG pAT261:Hly and BCG pMV306:Hly with actively-secreted hemolysin were constructed using chromosomal integration of a mycobacterial shuttle vector (pAT261 or PMV306) or by episomal plasmid replication, respectively (Hess et al., 1998; Hess et al., 1999). These plasmids were introduced into M. bovis BCG using standard electroporation. Fusion proteins were isolated and separated (Hess et al., 1998). In later studies, the fusion proteins were expressed by S. typhimurium strain SL7207's aroA pathway (Hess et al., 1999).
e. Virulence
The r-BCG has decreased intracellular persistence versus the parental BCG strain (Hess et al., 1999). Mycobacterial CFUs from infected macrophages were used to ascertain survival of BCG pAT261:Hly and BCG pMV306:Hly in host macrophages. Intracelluar survival of the BCG pMV306:Hly strain was reduced at day 1 post-infection, and viable BCG pAT261:Hly were not detected in J774A.1 macrophages at 15 days post-infection. Furthermore, the recombinant strains failed to escape from the phagosome of macrophages (Hess et al., 1998).
f. Description
A recombinant BCG strain was constructed to secrete a pore-forming sulfhydrul-activated cytolysin (listeriolysin or Hly) from Listeria monocytogenes. Listeriolysin assists Listeria in escaping from the phagosome into the cytosol of the infected host cell. The study shows that Hly secretion improves stimulation of CD8 T cells (by pronounced MHC class I processing and delivery) in recombinant BCG (Hess et al., 1999).
7. BCG Vaccine (Freeze-Dried)
a. Product Name:
BCG Vaccine (Freeze-Dried)
b. Manufacturer:
Sanofi Pasteur Ltd
c. Vaccine Ontology ID:
VO_0010708
d. Type:
Live, attenuated vaccine
e. Status:
Licensed
f. Location Licensed:
Canada
g. Host Species for Licensed Use:
Human
h. Immunization Route
Intradermal injection (i.d.)
i. Description
Products: Live bacteria. Other components: Polysorbate 80.
8. BCG-DHTM
a. Vaccine Ontology ID:
VO_0004618
b. Type:
Recombinant vector vaccine
c. Status:
Research
d. Antigen
BCG vaccine with deficient urease; a fusion protein composed of Mycobacterium tuberculosis-derived major membrane protein II (MMP-II) and heat shock protein 70 (HSP70) of BCG (Mukai et al., 2014).
e. Gene Engineering of DnaK
  • Type: Recombinant vector construction
  • Description: The M. tuberculosis gene dnaK, also known as hsp70, is a part of a fusion gene composed of the M. tuberculosis-derived MMP-II gene and the hsp70 gene of M. tuberculosis (locus tag: Rv0350). This fusion gene is introduced into the urease-deficient BCG-deltaUT-11-3 strain (Mukai et al., 2014).
  • Detailed Gene Information: Click here.
f. Gene Engineering of bfrA
  • Type: Recombinant vector construction
  • Description: The M. tuberculosis gene bfrA (also known as MMP-II) is a part of a fusion gene composed of the M. tuberculosis-derived MMP-II gene and the hsp70 gene of M. tuberculosis (locus tag: Rv0350). This fusion gene is introduced into the urease-deficient BCG-deltaUT-11-3 strain (Mukai et al., 2014).
  • Detailed Gene Information: Click here.
g. Immunization Route
subcutaneous injection
h. Mouse Response
  • Host Strain: C57BL/6J
  • Vaccination Protocol: Five mice per group were vaccinated with BCG-261H (control) or BCG-DHTM at 1000 CFU/mouse for 6 weeks (Mukai et al., 2014)
  • Vaccine Immune Response Type: VO_0003057
  • Challenge Protocol: Mice were challenged with H37RV at 100 CFU per lung by aerosol infection (Mukai et al., 2014)
  • Efficacy: At 6 weeks post challenge, mice vaccinated with BCG-261H (control) or BCG-DHTM showed inhibited multiplication of M. tuberculosis in the lung, and BCG-DHTM inhibited M. tuberculosis multiplication more strongly than BCG-261H (Mukai et al., 2014).
9. DNA vaccine co-expressing A85A and caspase-3
a. Vaccine Ontology ID:
VO_0004148
b. Type:
DNA vaccine
c. Antigen
The antigens for this vaccine were wild-type or mutated caspase-3 inserted into a plasmid that encodes mycolyl transferase antigen 85A (Ag85A) (Gartner et al., 2008).
d. Gene Engineering of Ag85A
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
e. Vector:
pBudCE4.1 (Gartner et al., 2008)
f. Preparation
Native Ag85A was purified from BCG culure and recombinant his-tagged Ag85A was purified by affinity chromatography. Dual expression-vectors were created encoding mature or secreted Ag85A and either mutant or wild-type caspase-3 (Gartner et al., 2008).
g. Mouse Response
  • Host Strain: (C57BL/6 × DBA/2)F1 (H-2b/d) or BALB/c (H-2d)
  • Vaccination Protocol: Mice were injected four times intramuscularly with 100 micrograms of pDNA with at least three weeks between injections. As negative controls, mice were injected with 100 micrograms of empty pBudCE4.1 vector or left untreated. As a positive control for the challenge experiments, mice were vaccinated intravenously with M. bovis BCG strain GL2 along with the first vaccination or 5 weeks pre-vaccination (Gartner et al., 2008).
  • Immune Response: Ag85A specific antibody titers were very low in mice vaccinated with the wild-type p-sAg85A–csp, but antibody titers in mice vaccinated with mutant p-sAg85A–csp were of a greater magnitude. IFN-γ and IL-2 production in mice vaccinated with plasmids co-expressing mAg85A and a caspase gene were similar to or lower than production in mice vaccinated with p-sAg85A. Vaccination with the wild-type caspase-encoding plasmids was not effective in inducing Ag85A specific CTL activity (Gartner et al., 2008).
  • Challenge Protocol: Mice were challenged five weeks after their final vaccination with 2 x 105 CFUof M. tuberculosis H37Rv. Results were measured using a luminescence assay with a count of relative light units (RLU) (Gartner et al., 2008).
  • Efficacy: The challenge revealed that mice vaccinated with mutant p-sAg85A–csp showed higher secretion of IL-6, IL-10 and IL-17A in their lungs, spontaneously and/or after antigenic stimulation.However, spontaneous IFN-γ production was highest in mice vaccinated with wild-type p-sAg85A–csp. Ag85A specific IL-2 and IFN-γ responses were low in spleen of BCG vaccinated mice, but high Th1 responses could be could be seen after stimulation with whole BCG culture filtrate in these mice (Gartner et al., 2008).
10. DNA vaccine Rv1806-1807
a. Vaccine Ontology ID:
VO_0000567
b. Type:
DNA vaccine
c. Antigen
Rv1806 and Rv1807 (Vipond et al., 2006).
d. Gene Engineering of Rv1806
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
e. Gene Engineering of Rv1807
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant: DDA vaccine adjuvant
g. Vector:
pET11d, modified to form pET3a (Vipond et al., 2006)
h. Preparation
Two bacterial strains, E. coli DH5α and Rosetta™ 2(DE3)pLysS, were propagated in Luria Bertani (LB) broth and solid medium at 37°C. An expression vector pET3a was created from pET11d expression vector (Novagen) by replacing an ampicillin cassette with kanamycin cassette, followed by restriction digestion of a HindIII site and linker insertion containing cloning sites and an N- and C-terminal 6* histidine tag. The vaccine candidate gene was amplified from M. tuberculosis using PCR and cloned into pVAX1tPA. Recombinant plasmids were generated from four encoding gene sequences and sub-cloned into pET3a prior to transformation, induction, and harvesting in E. coli Rosetta™ 2(DE3)pLysS (Vipond et al., 2006).
i. Virulence
Not virulent.
j. Description
Rv1806-1807 was one of three potential vaccines compared against gold-standard BCG vaccine and saline control. Rv1806-1807 induced protection in the guinea pig aerosol infection model 30 days post-challenge on the basis of reducing the bacterial burden of M tuberculosis in the lungs ( a reduction in log10 cfu of 0 . 52 and 0 . 83 in lungs and spleen , respectively , following DNA vaccination , compared with reductions of 0 . 3 and 0 . 57 , respectively , with a protein formulation) (Vipond et al., 2006).
k. Guinea pig Response
  • Host Strain: Dunkin-Hartley guinea pig
  • Vaccination Protocol: Female Dunkin-Hartley guinea pigs were vaccinated by injecting 100 μg of protein intramuscularly into both hind quadriceps muscles per guinea pig in 100 μl of dioctadecylammonium (DDA) and trehalose dibehenate (TDB) (500 μg DDA plus 100 μg TDB) stable liposomes (SSI). Sub-cutaneous inoculation with 5×104 CFU BCG Pasteur was used as positive control. Saline was used as negative control. Ten guinea pigs were used for each group. Vaccinated animals were booster vaccinated with 200 μg protein in adjuvant after 3 and 6 weeks (Vipond et al., 2006).
  • Challenge Protocol: Six weeks after the final vaccination, six animals per group were aerosol challenged with approximately 50 CFU of M. tuberculosis H37Rv (NCTC 7416) each in a contained Henderson apparatus. Protection was assessed by CFU in the lungs and spleen at 30 days post-challenge (Vipond et al., 2006).
  • Efficacy: Level of protection by Rv1806-1807 at week 15 post challenge was significantly better than saline (p=.018) and comparable to related DNA vaccinations. However, the protection was significantly less than gold-standard BCG. An antigen dose of 10 μg/ml of Rv1806-1807 vaccine stimulated the better protection than for 1 μg/ml (Vipond et al., 2006).
11. ΔureC hly+ rBCG
a. Vaccine Ontology ID:
VO_0000606
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Preparation
In order to generate a live vaccine that provides improved protection against M. tuberculosis, BCG was equipped with the membrane-perforating Hly of L. monocytogenes (hly+ rBCG). To improve the pH milieu for Hly activity, a ΔureC hly+ rBCG strain that lacked ureC activity was constructed (Grode et al., 2005).
e. Immunization Route
Intramuscular injection (i.m.)
f. Cattle Response
  • Vaccination Protocol: Mice were immunized i.v. with 106 CFU of BCG strains (Grode et al., 2005).
  • Challenge Protocol: At 120 days after vaccination, animals were aerosol challenged with 100–200 CFU of M. tuberculosis H37Rv or Beijing/W per lung using an aerosol chamber (Glas-Col) (Grode et al., 2005).
  • Efficacy: The ΔureC hly+ rBCG vaccine strain induced significantly more efficacious in inducing protective immunity against 2 different M. tuberculosis strains, the laboratory strain H37Rv and a clinical isolate of the Beijing/W genotype family, in a mouse model, than parental BCG (Grode et al., 2005). The ΔureC hly+ rBCG vaccine provided even more efficient protection than the hly+ rBCG vaccine strain.
12. LT-BCG- Ag85B /Rv3425
a. Vaccine Ontology ID:
VO_0004626
b. Type:
Recombinant vector vaccine
c. Status:
Research
d. Host Species for Licensed Use:
Baboon
e. Gene Engineering of Ag85B from M. tuberculosis H37Rv
  • Type: Recombinant vector construction
  • Description: DNA-, protein- and lentiviral vector-based vaccines that express the antigens Ag85B and Rv3425 (Xu et al., 2014).
  • Detailed Gene Information: Click here.
f. Vector:
(Xu et al., 2014)
g. Preparation
DNA-, protein- and lentiviral vector-based vaccines that express the antigens Ag85B and Rv3425 (Xu et al., 2014).
h. Immunization Route
Intramuscular injection (i.m.)
i. Mouse Response
  • Host Strain: C57BL/6
  • Vaccination Protocol: Six mice per group were first immunized subcutaneously (s.c.) with 5×106 CFU of BCG in 100μl of PBS and subsequently boosted twice at 2-week intervals with PAR, DAR or LAR [12]. Meanwhile, the mice were boosted with the controls for these three types of vaccines, which were PNC (i.e., MPL + TDM), DNC (i.e., pVax) and LNC (i.e.,pLenti6.3). For DAR immunization, 50μg of the DAR DNA vaccine in 100μl of sterile PBS was administered to the mice intramuscularly (i.m.) in the right thigh. For LAR immunization, the mice were immunized with 5×106 pfu of LAR in the foot pad. For PAR immunization, the mice were immunized subcutaneously (s.c.) with 50 μg of PAR formulated with the adjuvants MPL and TDM (Xu et al., 2014).
  • Vaccine Immune Response Type: VO_0003057
  • Challenge Protocol: 2 weeks after the last vaccination, all mice were challenged intravenously via the lateral tail vein with 1.2 × 106 CFU of M. tuberculosis H37Rv (Xu et al., 2014).
  • Efficacy: Prime-boost BCG vaccination with a lentiviral vector expressing the antigens Ag85B and Rv3425 significantly enhanced immune responses, including Th1 and CD8+ CTL responses, compared to DNA- and protein-based vaccines. However, lentivirus-vectored and DNA-based vaccines greatly improved the protective efficacy of BCG against M. tuberculosis (Xu et al., 2014).
13. M. bovis WAg520 strain
a. Vaccine Ontology ID:
VO_0000552
b. Type:
Attenuated vaccine
c. Antigen
The antigen for this vaccine was an attenuated strain of M. bovis called WAg520, which cantains a deletion in a possible undecaprenol kinase gene (De et al., 1999).
d. Preparation
WAg520 was one of four nutritionally impaired strains of M. bovis selected from 440 M. bovis mutants produced by illegitimate recombination based upon their inability to grow in minimal medium (De et al., 1999).
e. Virulence
This strain, along with the other test M. bovis mutants, was shown to be avirulent for guinea pigs following the subcutaneous inoculation (De et al., 1999).
f. Description
WAg520, an attenuated strain of M. bovis, has only a 2-bp deletion in a possible undecaprenol kinase gene. It is one of two nutritionally impaired strains which produced significant protection in the lungs. The other one is strain WAg522 (De et al., 1999).
g. Guinea pig Response
  • Host Strain: Duncan-Hartley
  • Vaccination Protocol: Four to eight guinea pigs per group were vaccinated by subcutaneous injection with 105 CFU with one of the four impaired M. bovis strains or BCG. Ten animals were not vaccinated (control) (De et al., 1999).
  • Side Effects: No delayed-type hypersensitivity reactions observed prior to inoculation with mycobacteria (De et al., 1999). Authors were unable to determine if nutritionally-impaired mutants influenced the development of necrotizing lesions in the lungs (De et al., 1999).
  • Challenge Protocol: All animals were anesthetized eight weeks post-vaccination via intramuscular injection with 3 mg ketamine hydrochloride, followed by 10x2 CFU virulent M. bovis WAg201 introduced through mouth into the trachea via cannula. Three guinea pigs which were not challeged received 105 CFU of each of the four strains via subcutaneous innoculation (De et al., 1999).
  • Efficacy: Significant differences in lung CFU were observed between vaccinated groups and WAg520 & WAg522 strains (De et al., 1999). WAg520 elicited 0.92, 3.83, and 2.47 log10 increases in lung, splenic, and liver tissues, respectively (Table I, (De et al., 1999)). Macroscopic lesions were decreased in WAg520 recipients versus both non-vaccinated (significant different) and BCG-vaccinated (not significantly different) animals (Table 2, (De et al., 1999)).
  • Description: All animals were sacrificed 13 weeks post-vaccination.
14. M. bovis WAg522 strain
a. Vaccine Ontology ID:
VO_0000557
b. Type:
Attenuated vaccine
c. Antigen
The antigen for this vaccine was an attenuated strain of M. bovis called WAg522 (De et al., 1999).
d. Preparation
WAg522 is the second of four M. bovis mutants selected from 440 illegitimate recombinant M. bovis mutants via their inability to grow in minimal medium (De et al., 1999).
e. Virulence
This strain, along with the other tested M. bovis mutants in (De et al., 1999), was shown to be avirulent in guinea pigs following subcutaneous inoculation. Furthermore, the groups treated with WAg522 and WAg520 strains both showed significant differences in lung CFU versus nonvaccinated groups.
f. Guinea pig Response
  • Host Strain: Duncan-Hartley
  • Vaccination Protocol: Four to eight guinea pigs per group were vaccinated by subcutaneous injection with 105 CFU with one of the four impaired M. bovis strains or BCG. Ten animals were not vaccinated (control) (De et al., 1999).
  • Side Effects: No delayed-type hypersensitivity reactions observed prior to inoculation with mycobacteria (De et al., 1999). Authors were unable to determine if nutritionally-impaired mutants influenced the development of necrotizing lesions in the lungs (De et al., 1999).
  • Challenge Protocol: All animals were anesthetized eight weeks post-vaccination via intramuscular injection with 3 mg ketamine hydrochloride, followed by 10x2 CFU virulent M. bovis WAg201 introduced through mouth into the trachea via cannula. Three guinea pigs which were not challeged received 105 CFU of each of the four strains via subcutaneous innoculation (De et al., 1999).
  • Efficacy: Significant differences in lung CFU were observed between vaccinated groups and WAg520 & WAg522 strains (De et al., 1999). WAg520 elicited 0.92, 3.83, and 2.47 log10 increases in lung, splenic, and liver tissues, respectively (see Table I, (De et al., 1999)). Macroscopic lesions were decreased in WAg520 recipients versus both non-vaccinated (significant different) and BCG-vaccinated (not significantly different) animals (see Table 2, (De et al., 1999)).
15. M. tuberculosis DNA vaccine (containing the ESAT-6, MPT-64, MPT-83, and KatG constructs)
a. Vaccine Ontology ID:
VO_0004363
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of MPT64
f. Gene Engineering of MPT83
g. Gene Engineering of KatG
h. Vector:
pJW4303 (Morris et al., 2000)
i. Immunization Route
Intramuscular injection (i.m.)
j. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: The relative protection in the lung induced by the combination vaccine approached the BCG response in these experiments (Morris et al., 2000).
16. M. tuberculosis DNA Vaccine APADNA priming and APAMVA boosting
a. Vaccine Ontology ID:
VO_0011478
b. Type:
Other
c. Status:
Research
d. Gene Engineering of Apa
  • Type: Recombinant vector construction
  • Description:
  • Detailed Gene Information: Click here.
e. Vector:
pUMVC3 and poxvirus (Kumar et al., 2003).
f. Immunization Route
Intradermal injection (i.d.)
g. Guinea pig Response
  • Vaccination Protocol: Animals were immunized intradermally with APADNA, 200 μg, given three times, 4 weeks apart (at weeks 0, 4, and 8), followed by APAMVA, 107 PFU, given 4 weeks after the third DNA (at 12 weeks); or BCG Guindy, 106 CFU, given one time only (at week 0) or control DNA, 200 μg, given three times, 4 weeks apart (at weeks 0, 4, and 8), followed by MVA wild type, 107 PFU, given 4 weeks after the third DNA (at week 12) (Kumar et al., 2003).
  • Challenge Protocol: Animals were challenged intramuscularly in the thigh muscle at 4 weeks after the last immunization with 2 × 105 viable M. tuberculosis strain NTI64719. This challenge dose was sufficient to cause measurable clinical illness in all control animals within a relatively short time frame of 6 weeks (Kumar et al., 2003).
  • Efficacy: A poxvirus recombinant expressing the Apa protein conferred a significant level of protective immunity in guinea pigs against a challenge dose of virulent M. tuberculosis (Kumar et al., 2003).
17. M. tuberculosis DNA vaccine DNAAg85A encoding a single immunogenic M.tb Ag
a. Vaccine Ontology ID:
VO_0004364
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of Ag85A
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3.1 expressed a single immunogenic M.tb Ag (Ag85A) (Wang et al., 2004).
  • Detailed Gene Information: Click here.
f. Vector:
pCDNA3.1 (Wang et al., 2004)
g. Immunization Route
intranasal immunization
h. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: Systemic priming with two repeated DNAAg85A injections and respiratory mucosal boosting once with AdAg85A provided the most potent protection of all of the tested vaccines/regimens, including s.c. BCG vaccination both at 4 and 8 wk postchallenge, and this level of protection was also better than single i.n. AdAg85A vaccination at 8 wk postchallenge (Wang et al., 2004).
18. M. tuberculosis DNA Vaccine encoding Ag85B Protein
a. Vaccine Ontology ID:
VO_0011410
b. Type:
DNA vaccine
c. Status:
Research
d. Gene Engineering of FbpB from M. tuberculosis
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
e. Vector:
pRC (Palma et al., 2007)
f. Immunization Route
Intramuscular injection (i.m.)
g. Mouse Response
  • Host Strain: C57BL/6
  • Vaccination Protocol: Mice at or between 7 and 8 weeks old were immunized. Fifty micrograms of plasmid, both DNA-85B or control vector, was injected intramuscularly (i.m.) in 50 μl PBS into the hind leg. Mice were immunized two or four times at 2 week intervals. As a positive control, a single dose of BCG (105 cfu) was injected s.c. (Palma et al., 2007).
  • Challenge Protocol: Four weeks after the last boost, mice were challenged intravenously (i.v.) in a lateral tail vein with 105 cfu of MTB H37Rv (Palma et al., 2007).
  • Efficacy: A significant reduction of the bacterial load was observed in mice receiving two administrations of the DNA-85B vaccine (DNAx2-immunized mice) with respect to unvaccinated naïve control or empty-plasmid DNA recipient mice, indicating protection by the vaccine. The degree of reduction of the mycobacterial burden was not significantly different from that conferred by BCG, although the immunization with BCG caused the highest nominal reduction of mycobacteria in the lungs (Palma et al., 2007).
19. M. tuberculosis DNA vaccine encoding Ag85B, MPT64 and MPT83
a. Vaccine Ontology ID:
VO_0004362
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of Ag85B from M. tuberculosis H37Rv
  • Type: DNA vaccine construction
  • Description: Vector pJW4303 expressed Ag85B of H37Rv (Cai et al., 2005).
  • Detailed Gene Information: Click here.
f. Gene Engineering of MPT64
  • Type: DNA vaccine construction
  • Description: Vector pJW4303 expressed MPT64 of H37Rv (Cai et al., 2005)
  • Detailed Gene Information: Click here.
g. Gene Engineering of MPT83
  • Type: DNA vaccine construction
  • Description: Vector pJW4303 expressed MPT83 of H37Rv (Cai et al., 2005).
  • Detailed Gene Information: Click here.
h. Vector:
pJW4303 (Cai et al., 2005)
i. Immunization Route
Intramuscular injection (i.m.)
j. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: Combined DNA vaccines including Ag85B, MPT64, and MPT83 formulated in IL-2 provided an efficient method for generating strong and sustained protective immunity in the spleen and lungs of experimental mice following i.v. challenge with M. tuberculosis H37Rv (Cai et al., 2005).
20. M. tuberculosis DNA Vaccine encoding KatG
a. Vaccine Ontology ID:
VO_0004058
b. Type:
DNA vaccine
c. Status:
Research
d. Gene Engineering of KatG
  • Type: DNA vaccine construction
  • Description: KatG protein was amplified from M. tuberculosis H37Rv chromosomal DNA by PCR, and then cloned into the eukaryotic expression vector pJW4303 (Li et al., 1999).
  • Detailed Gene Information: Click here.
e. Vector:
The eukaryotic expression vector pJW4303
f. Immunization Route
Intramuscular injection (i.m.)
g. Mouse Response
  • Host Strain: C57BL/6
  • Vaccination Protocol: The C57BL/6 mice (five mice per group) were vaccinated intramuscularly into the thigh three times at 3-week intervals, with 100 μg of plasmid DNA per injection. Control mice were vaccinated with 106 CFU of BCG Pasteur injected subcutaneously (Li et al., 1999).
  • Challenge Protocol: The mice were challenged by the aerosol route about 17 to 21 days after receiving the final DNA vaccine inoculation and 6 weeks after receiving the BCG vaccination with M. tuberculosis Erdman (Li et al., 1999).
  • Efficacy: Vaccination with the KatG resulted in a 75 to 85% drop in number of lung bacteria. Moreover, mice immunized with the KatG TPA-positive constructs showed significantly decreased dissemination of the lung infection to the spleen (Li et al., 1999).
21. M. tuberculosis DNA vaccine ESAT-6
a. Vaccine Ontology ID:
VO_0004035
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Vector:
pJW4303 (Morris et al., 2000)
f. Immunization Route
Intramuscular injection (i.m.)
g. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: Immunization with a multivalent combination DNA vaccine (containing the ESAT-6, MPT-64, MPT-63, and KatG constructs) generated immune responses that indicated an absence of antigenic competition since antigen-specific cell-mediated and humoral responses were detected to each component of the mixture (Morris et al., 2000).
  • Efficacy: Prior to vaccination, mice were challenged with M. tuberculosis. Among the single DNA vaccines that were tested, the ESAT-6 construct consistently evoked the best protective responses. Compared with BCG, which on a relative scale elicited a protective value of 1, ESAT-6 elicited a protective value of 0.61 (Morris et al., 2000).
22. M. tuberculosis DNA vaccine pAK4-sod
a. Vaccine Ontology ID:
VO_0004123
b. Type:
DNA vaccine
c. Antigen
mycobacterial antigen superoxide dismutase A (Rv3846).
d. Gene Engineering of SodA
  • Type: DNA vaccine preparation
  • Description: The gene sodA was subcloned into the expression vectors pAK3 and pAK4 (Khera et al., 2005).
  • Detailed Gene Information: Click here.
e. Vector:
pAK4 (Khera et al., 2005)
f. Preparation
PCR amplified using gene specific primers containing Bgl II overhangs and M. tuberculosis genomic DNA as template. The blunt end PCR amplicon was cloned into EcoR V digested pLitmus-38, and then subcloned into plasmids pAK3 and pAK4 resulting in pAK3-sod and pAK4-sod. While both pAK3 and pAK4 are the eukaryotic expression vectors, pAK4 contains more CpG motifs. pAK4 also carries the ampicillin resistance gene carrying two immunostimulatory sequences. pAK3 is devoid of this ampicillin gene (Khera et al., 2005).
g. Virulence
None
h. Description
A study compared DNA vaccines expressing mycobacterial antigens ESAT-6 (Rv3875), α-crystallin (Rv2031c) and superoxide dismutase A (Rv3846) in Balb/c mice and guinea pigs. The DNA vaccine expression sodA was found to offer maximum protection (Khera et al., 2005).
i. Mouse Response
  • Host Strain: Balb/c mice
  • Vaccination Protocol: Mice (in groups of six each) were immunized with either the pAK3 or pAK4 vector controls or DNA vaccine candidates. Three doses of 100 μg of plasmid DNA were administered intramuscularly at 3-week intervals. Following a rest period of 3 weeks after the last dose, mice were euthanized, and antigen specific cell mediated and humoral immune responses were evaluated (Khera et al., 2005).
  • Immune Response: Immunization of mice with the DNA vaccines expressing superoxide dismutase A resulted in markedly higher levels of IFN-γ as compared to the levels of IL-10. The levels of IFN-γ in supernatants from pAK3-sod and pAK4-sod immunized mice increased 2.9 and 9.1 folds compared to the levels of IFN-γ in culture supernatant of mice immunized with pAK-3 and pAK-4, respectively. Approximately 3-fold increase in IFN-γ levels in pAK4-sod immunized mice in comparison to pAK3-sod immunized mice showed that the extra CpG motif activated Th1 subtype of host immunity. Though expression of SOD antigen increases IL-10 levels, the extra CpG motifs in the vector backbone decreases the concentration of IL-10 in pAK4-sod immunized mice in comparison to pAK3-sod immunized mice (Khera et al., 2005).
  • Challenge Protocol: No challenge experiment was performed in mice. Guinea pigs were used for protection assay for this vaccine.
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: The levels of IFN-γ in supernatants from pAK3-sod and pAK4-sod immunized mice significantly increased compared to the plasmid vector control groups (Khera et al., 2005).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Il10 (interleukin 10)
    • Gene Response: Compared to the plasmid pAK3 and pAK4 controls, expression of the SODA antigen increased IL-10 levels in DNA vaccine pAK3-sod and pAK4-sod immunized mice. The extra CpG motifs in the pAK4 plasmid decreases the IL-10 level in pAK4-sod immunized mice compared to pAK3-sod immunized mice (Khera et al., 2005).
    • Detailed Gene Information: Click here.
j. Guinea pig Response
  • Host Strain: Outbred guinea pigs of the Duncan Hartley strain
  • Challenge Protocol: Guinea pigs (in groups of six) were immunized intramuscularly with three doses (of 100 μg each) of pAK4, pAK4-E6 expressing ESAT-6, pAK4-αcry expressing α-crystallin, or pAK4-sod at 3 weeks intervals. Four weeks after the last booster, guinea pigs were subcutaneously challenged with 1 × 105 CFU of M. tuberculosis H37Rv. Guinea pigs were euthanised 4 weeks after challenge (Khera et al., 2005).
  • Efficacy: DNA vaccine expressing superoxide dismutase imparted the maximum protection as observed by a 50 and 10 folds reduction in bacillary load in spleens and lungs, respectively, in comparison to immunization with vector control. Among the animals vaccinated with various DNA vaccines, pAK4-sod immunized guinea pigs exhibited the minimal granuloma (Khera et al., 2005).
23. M. tuberculosis DNA vaccine pcDNA3.1+/Ag85A DNA encoding Ag85A
a. Vaccine Ontology ID:
VO_0004356
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of Ag85A
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3.1 expressed Mycobacterium antigen 85A (Ag85A) (Wang et al., 2010).
  • Detailed Gene Information: Click here.
f. Vector:
pcDNA3.1 (Wang et al., 2010)
g. Immunization Route
Intramuscular injection (i.m.)
h. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: Orally administrated liposomal-pcDNA3.1+/Ag85A DNA was efficiently incorporated into mucosal epithelium of the small intestine, Peyer's patches (PP). This initiated a Ag85A-specific Th1 dominant immune response, as evidenced by increased secretion of IL-2, IFN-γ, and no change of IL-4 (Wang et al., 2010)
  • Efficacy: Liposomal-pcDNA3.1+/Ag85A DNA significantly increased the protection by giving a markedly reduction of TB burden in the lung, demonstrating that the TB-specific immune responses elicited by oral administration of liposomal-pcDNA3.1+/Ag85A DNA led to an enhanced host defense against TB infection. (Wang et al., 2010)
24. M. tuberculosis HBHA Protein Vaccine
a. Vaccine Ontology ID:
VO_0004059
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
HBHA
e. Gene Engineering of HBHA
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant: MPL vaccine adjuvant
g. Adjuvant: DDA vaccine adjuvant
h. Immunization Route
subcutaneous injection
i. Mouse Response
  • Host Strain: C57BL/6
  • Vaccination Protocol: were vaccinated subcutaneously three times with 5 μg of either HPLC-purified HBHA (nHBHA) or recombinant HBHA emulsified in 150 μg of dimethyldioctadecylammonium bromide (DDA) (Acros Organics, Morris Plains, N.J.)-monophosphoryl lipid A (MPL) adjuvant or with the adjuvant combination alone (Parra et al., 2004).
  • Challenge Protocol: Mice were challenged with aerosolized M. tuberculosis Erdman (200 CFU) 4 weeks following the final immunization (Parra et al., 2004).
  • Efficacy: The HBHA-containing vaccine gave a approximately 0.7-log reduction in CFU in both mouse lungs and spleens compared to adjuvant controls 28 days following challenge (Parra et al., 2004).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: