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Salmonella spp.

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Disease
    3. Introduction
    4. Microbial Pathogenesis
    5. Host Ranges and Animal Models
    6. Host Protective Immunity
  2. Vaccine Related Pathogen Genes
    1. aroA
    2. aroa
    3. crp
    4. dsd
    5. Frag C
    6. HtrA
    7. TviA
    8. Ty2
    9. fsd (Defense factor)
    10. DnaJ (Protective antigen)
    11. GroEL (Protective antigen)
    12. HslU (Protective antigen)
    13. IroN (Protective antigen)
    14. SopB (Protective antigen)
    15. TviB (Protective antigen)
    16. aroA (Virmugen)
    17. AroC (Virmugen)
    18. AroD (Virmugen)
    19. asd (Virmugen)
    20. atpA (Virmugen)
    21. cbiA (Virmugen)
    22. clpP (Virmugen)
    23. clpP from S. typhimurium str. LT2 (Virmugen)
    24. cobS (Virmugen)
    25. cpxA (Virmugen)
    26. cpxR (Virmugen)
    27. crp (Virmugen)
    28. crp from S. Typhimurium (Virmugen)
    29. cya (Virmugen)
    30. fliC (Virmugen)
    31. fliD (Virmugen)
    32. galE (Virmugen)
    33. guaA (Virmugen)
    34. guaA from S. typhimurium str. LT2 (Virmugen)
    35. guaB (Virmugen)
    36. guaB from S. enteritidis str. P125109 (Virmugen)
    37. gyrA (Virmugen)
    38. hfq (Virmugen)
    39. htrA (Virmugen)
    40. lon (Virmugen)
    41. lppA (Virmugen)
    42. lppB (Virmugen)
    43. msbB (Virmugen)
    44. NmpC (Virmugen)
    45. nuoG (Virmugen)
    46. ompR (Virmugen)
    47. PhoP (Virmugen)
    48. poxA (Virmugen)
    49. relA (Virmugen)
    50. rfc (Virmugen)
    51. rpoB (Virmugen)
    52. rpoS (Virmugen)
    53. ruvB (Virmugen)
    54. spoT (Virmugen)
    55. surA (Virmugen)
    56. tolA (Virmugen)
    57. trxA (Virmugen)
    58. wecA (Virmugen)
    59. znuA (Virmugen)
    60. znuB (Virmugen)
    61. znuC (Virmugen)
  3. Vaccine Related Host Genes
    1. Ifng (Interferon gamma)
    2. IgA
    3. IgA
    4. IgA Fc receptor
    5. IgG
    6. IgG Fc fragment
    7. IgG2b
    8. Ighg1
    9. Ighv1-9
    10. IgM
    11. IgM
    12. IgM Fc receptor
    13. IL-17
    14. IL-6
  4. Vaccine Information
    1. Aro-deleted S. Typhi
    2. Bovine Rhinotracheitis Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 4109.20)
    3. Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 43T5.20)
    4. Broilact Chick Salmonella vaccine
    5. CVD 908-htrA
    6. CVD 909
    7. CVD 915
    8. Megan Egg
    9. Megan Vac1
    10. Newcastle Disease-Bronchitis Mass & Ark Types, Killed Virus Vaccine-Salmonella Enteritidis Bacterin (USDA: 48D7.10)
    11. Newcastle Disease-Bronchitis Mass & Ark Types, Killed Virus Vaccine-Salmonella Enteritidis Bacterin (USDA: 48D7.11)
    12. Newcastle-Bronchitis Mass Type, Killed Virus Vaccine-Salmonella Enteritidis Bacterin (USDA: 48D6.10)
    13. S. typhi DnaJ Protein Vaccine
    14. S. typhi GroEL Protein Vaccine
    15. S. typimurium Vi4072
    16. Salmonella Choleraesuis Avirulent Live Culture Vaccine (USDA: 19A1.00)
    17. Salmonella Choleraesuis Avirulent Live Culture Vaccine (USDA: 19A1.01)
    18. Salmonella DNA vaccine encoding SopB Protein
    19. Salmonella Dublin Live Culture Vaccine (USDA: 19D1.R0)
    20. Salmonella enterica fliC/guaB mutant vaccine
    21. Salmonella Enteritidis guaBA/clpP mutant vaccine
    22. Salmonella Gallinarum cbiA/cobS mutant vaccine
    23. Salmonella Gallinarum cpxR/Ion mutant vaccine
    24. Salmonella Gallinarum crp mutant vaccine
    25. Salmonella Gallinarum nuoG mutant vaccine
    26. Salmonella Gallinarum relA/spoT mutant vaccine
    27. Salmonella IroN Protein Vaccine
    28. Salmonella typhi aroA mutant vaccine
    29. Salmonella Typhi galE mutant vaccine
    30. Salmonella Typhi phoP mutant vaccine
    31. Salmonella Typhimurium asd mutant vaccine
    32. Salmonella Typhimurium asd/rfc mutant vaccine
    33. Salmonella Typhimurium atpA mutant vaccine
    34. Salmonella Typhimurium clpP mutant vaccine
    35. Salmonella Typhimurium cya/crp mutant vaccine
    36. Salmonella Typhimurium guaBA/clpP mutant vaccine
    37. Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine
    38. Salmonella Typhimurium gyrA/cpxA/rpoB mutant vaccine
    39. Salmonella Typhimurium hfq mutant vaccine
    40. Salmonella Typhimurium htrA mutant vaccine
    41. Salmonella Typhimurium IppA/IppB/msbB
    42. Salmonella Typhimurium IppB/msbB mutant vaccine
    43. Salmonella Typhimurium Live Culture Vaccine (USDA: 19C1.00)
    44. Salmonella Typhimurium Live Culture Vaccine (USDA: 19C1.01)
    45. Salmonella Typhimurium Live Culture Vaccine (USDA: 19C1.02)
    46. Salmonella Typhimurium ompD/nmpC mutant vaccine
    47. Salmonella Typhimurium ompR mutant vaccine
    48. Salmonella Typhimurium poxA mutant vaccine
    49. Salmonella Typhimurium rpoS mutant vaccine
    50. Salmonella Typhimurium ruvB mutant vaccine
    51. Salmonella Typhimurium surA mutant vaccine
    52. Salmonella Typhimurium tolA mutant vaccine
    53. Salmonella Typhimurium trxA mutant vaccine
    54. Salmonella Typhimurium wecA mutant vaccine
    55. Salmonella Typhimurium znuABC mutant vaccine
    56. Salmonella vacT
    57. Typherix
    58. Typhim Vi
    59. ViVaxim
    60. Vivotif
    61. Vivotif L
    62. Zoosaloral H.
  5. References
I. General Information
1. NCBI Taxonomy ID:
590
2. Disease:
Salmonellosis
3. Introduction
Salmonella serovars, like Salmonella enterica serovars Paratyphi and Typhi, cause an estimated 200,000 deaths per year. Much of it is spread by contaminated food or drinking water. Salmonella disease is mostly found in Africa, South America, and Southeast Asia. In Southeast Asia, the prevalence of typhoidal salmonellosis is high and many patients suffer relapses. Studies are being done to see why the immune system is unable to mount a lasting defense against the bacteria, and how to counteract antibiotic resistance, one of the main difficulties encountered in the protection of the Salmonella vaccine (Boyle et al., 2007).
4. Microbial Pathogenesis
Salmonella first replicates in epithelial cells. It generally targets dendritic cells and macrophages after the mucosal barrier, where it multiplies rapidly (Cheminay and Hensel, 2007). Salmonella infections induce a weak and delayed T cell responses in human typhoid fever and in animal models of typhoid fever, suggesting immunosuppressive effects. Salmonella also directly inhibits T cell proliferations (Cheminay and Hensel, 2007).
5. Host Ranges and Animal Models
S. Typhi only causes infections in humans. S. Typhimurium causes a lethal disease in susceptible mice which resembles in many aspects of human typoid fever. Therefore, S. Typhimurium has been frequently used as a model to study human typhoid fever. S. enterica causes zoonotic gastrointestinal infections from reservoirs in all important livestock animals. Vaccine studies have been done on humans, cattle, mice, and chickens (Boyle et al., 2007; Boyle et al., 2007).
6. Host Protective Immunity
Little information exists regarding the protective immunity and immunological memory for serovar Typhi. B-cell production of soluble antibodies, cytotoxic T lymphocyte activities for killing infected cells, CD4+ and CD8+ T cell homing to the gut, and distinct subsets of memory T cells are shown to be essential for protective immunity against serovar Typhi infection (Boyle et al., 2007). Two vaccines are currently commercially available against typhoid. Typhim Vi (Pasteur Merieux) is a vaccine of purified Vi-antigen from S. Typhi. Vivotif is a live attenuated S. Typhi Ty21 strain, generated by the mutagenesis of the virulent, parental S. Typhi Ty2. The major challenge of vaccine development is balancing immunogenicity and attenuation. Many vaccines have been developed with mutations in regulatory genes encoding adenylate cyclase and cAMP receptor protien (Cheminay and Hensel, 2007).
1. aroA
  • Gene Name : aroA
2. aroA
  • Gene Name : aroA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. Ty2
  • NCBI Gene ID : 1068996
  • NCBI Protein GI : 29142379
  • Locus Tag : t1956
  • Genbank Accession : AE014613
  • Protein Accession : NP_805721
  • Taxonomy ID : 209261
  • Gene Starting Position : 2006648
  • Gene Ending Position : 2007931
  • Gene Strand (Orientation) : -
  • Protein Name : 3-phosphoshikimate 1-carboxyvinyltransferase
  • Protein pI : 6.35
  • Protein Weight : 44035.02
  • Protein Length : 427
  • Protein Note : catalyzes the formation of 5-O-(1-carboxyvinyl)-3-phosphoshikimate from phosphoenolpyruvate and 3-phosphoshikimate in tryptophan biosynthesis
  • DNA Sequence : Show Sequence
    >gi|29140543:2006648-2007931 Salmonella enterica subsp. enterica serovar Typhi str. Ty2 chromosome, complete genome
    ATTAGGCAGGCGTACTCATCCGCGCCAGTTGTTCGAAATAATCAGGGAACGTTTTTGCGGTACATTTAGG
    GTCCAGGATCGTGACTGGCGTATCGGACAGTGCGACCAGTGAGAAACACATCGCCATACGGTGGTCGTTG
    TACGTGCCAATATCCGCGTGTTGGAGCTTCGCCGGCGGCGTGATACGAATATAGTCGTGCCCTTCTTCGA
    CTTCAGCGCCCACTTTACGTAGCTCGGTCGCCATCGCGAACAGGCGATCGGTTTCTTTCACTCGCCAGTT
    ATAAATATTGCGCAACGTCGTGGTTCCTTTCGCAAACAACGCCGTGGTGGCAATCGTCATCGCCGCATCC
    GGAATATGGTTCATATCCATATCTATGGCGTGCAATTCGCCGCGCGTGCAGGCAATAAAATCATCGCCCC
    AGGTAATGGTCGCGCCCATTTTCTCCAGCACATCGGCAAAACGAATATCGCCCTGCATACTTTTGCGGCC
    AATCCCGGTCACTTTTACCGTGCCGCCTTTTATCGCCCCAGCGGCGAGAAAATAGGACGCTGACGAGGCA
    TCGCCCTCGACCAGATAGCGACCTGGAGAGTGATACTGTTGACCGCCCTTCACGACAAATTGTTGGTAGT
    GATGGTTCGCTATCTCCACGCCAAAGGTTTTCATTAAATTTAGCGTGATATCGATGTAAGGTTTTGATAC
    CAGTTCGCCTTTAACGCGAATAATTGTGTCTTCAGGCGCCAGCGGCGCCGTCATCAGCAGAGCGGTCAGG
    AACTGGCTGGAAACGCTACCATCAACCTCAATGTCGCCGCCGATAAAACCGCCGCGCAGACGCAGGGGCG
    GATAGTTTTCCTGCTCCAGGTAATCAATATTCGCCCCACCCTGACGCAGCGAATCGACCAGATGGCCTAT
    CGGACGCTCTTTCATACGCGGTTCGCCGGTTAACACTATCTCATTTTGCCCCAGACATAGCGCTGCCGCT
    AACGGACGCATCGCGGTTCCGGCATTACCGAGAAACAGTTCCAGAGTGCCTGACGCGCGTAATGGGCCGC
    CATTACCCGTGATATCACAGCGGGTGCGATCGGCAGAAAGGGTGTAATTGATCCCCAACGCGCTCAGGGC
    ATTGAGCATATGGCGGACGTCATCGCTATCCAGCAGATTCGTCAGAACGGTTTTACCACAAGCTAAAGCC
    GCCAGGAGCAAAGCACGGTTTGAAACACTTTTGGAGCCAGGTAAATTAATGGCGCCATCGACCCGCGCGA
    TGGGTTGTAACGTCAGGGATTCCA
  • Protein Sequence : Show Sequence
    >gi|29142379|ref|NP_805721.1| 3-phosphoshikimate 1-carboxyvinyltransferase [Salmonella enterica subsp. enterica serovar Typhi str. Ty2]
    MESLTLQPIARVDGAINLPGSKSVSNRALLLAALACGKTVLTNLLDSDDVRHMLNALSALGINYTLSADR
    TRCDITGNGGPLRASGTLELFLGNAGTAMRPLAAALCLGQNEIVLTGEPRMKERPIGHLVDSLRQGGANI
    DYLEQENYPPLRLRGGFIGGDIEVDGSVSSQFLTALLMTAPLAPEDTIIRVKGELVSKPYIDITLNLMKT
    FGVEIANHHYQQFVVKGGQQYHSPGRYLVEGDASSASYFLAAGAIKGGTVKVTGIGRKSMQGDIRFADVL
    EKMGATITWGDDFIACTRGELHAIDMDMNHIPDAAMTIATTALFAKGTTTLRNIYNWRVKETDRLFAMAT
    ELRKVGAEVEEGHDYIRITPPAKLQHADIGTYNDHRMAMCFSLVALSDTPVTILDPKCTAKTFPDYFEQL
    ARMSTPA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An aroA mutant is attenuated in mice and induced significant protection from challenge with wild type S. typhi Ty2 (Dougan et al., 1987).
  • Related Vaccine(s): Salmonella typhi aroA mutant vaccine
3. aroa
  • Gene Name : aroa
4. AroC
  • Gene Name : AroC
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi Ty2
  • NCBI Gene ID : 1069415
  • NCBI Protein GI : 29140996
  • Locus Tag : t0480
  • Genbank Accession : NC_004631.1
  • Protein Accession : NP_804338.1
  • Taxonomy ID : 28901
  • Gene Starting Position : 554066
  • Gene Ending Position : 555151
  • Gene Strand (Orientation) : +
  • Protein Name : chorismate synthase
  • Protein pI : 6.68
  • Protein Weight : 39122.2
  • Protein Length : 361
  • Protein Note : catalyzes the formation of chorismate from 5-O-(1-carboxyvinyl)-3-phosphoshikimate in aromatic amino acid biosynthesis
  • DNA Sequence : Show Sequence
    >GeneID|1069415 [Salmonella enterica subsp. enterica serovar Typhi Ty2 ] 554066..555151
    tggcaggaaacacaattggacaactctttcgcgtaaccactttcggcgaatcacacgggctggcgcttgg
    gggtatcgtcgatggcgtgccgcccggcatcccgttgacggaggccgatctgcagcacgatctcgacaga
    cgccgccctggcacctcgcgctatactactcagcgccgcgaaccggaccaggtaaaaattctctccggcg
    tgtttgatggcgtgacgaccggcaccagtattggcctgctgattgaaaacaccgatcagcgctcgcagga
    ctacagcgcgattaaagatgtttttcgcccgggacacgcggattacacctatgagcagaaatacggcctg
    cgcgattaccgcggcggtggacgttcttccgcgcgtgaaaccgcgatgcgcgtagcggcaggggcgatcg
    ccaagaaatacttggcggaaaagttcggcatcgaaatccgcggctgcctgacccagatgggcgacattcc
    gctggagattaaagactggcgtcaggttgagcttaatccgttcttttgccccgatgcggacaaacttgac
    gcgctggacgaactgatgcgcgcgctgaaaaaagagggtgactccatcggcgcgaaagtgacggtgatgg
    cgagcggcgtgccggcagggcttggcgaaccggtatttgaccgactggatgcggacatcgcccatgcgct
    gatgagcatcaatgcggtgaaaggcgtggagatcggcgaaggatttaacgtggtggcgctgcgcggcagc
    cagaatcgcgatgaaatcacggcgcagggttttcagagcaaccacgctggcggcatcctcggtggcatca
    gtagtgggcaacacattgtggcgcatatggcgctgaaacctacctccagcattaccgtgccgggacgtac
    gatcaaccggatgggtgaagaggtcgagatgatcaccaaagggcgccacgatccgtgtgtggggattcgc
    gcagtgccgatcgcagaagccatgctggcgatcgtactgatggatcacctgctgcgccatcgggcacaga
    atgcggatgtaaagacagagattccacgctggtaag
  • Protein Sequence : Show Sequence
    >gi|29140996|ref|NP_804338.1| chorismate synthase [Salmonella enterica subsp. enterica serovar Typhi Ty2 ]
    MAGNTIGQLFRVTTFGESHGLALGGIVDGVPPGIPLTEADLQHDLDRRRPGTSRYTTQRREPDQVKILSG
    VFDGVTTGTSIGLLIENTDQRSQDYSAIKDVFRPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAI
    AKKYLAEKFGIEIRGCLTQMGDIPLEIKDWRQVELNPFFCPDADKLDALDELMRALKKEGDSIGAKVTVM
    ASGVPAGLGEPVFDRLDADIAHALMSINAVKGVEIGEGFNVVALRGSQNRDEITAQGFQSNHAGGILGGI
    SSGQHIVAHMALKPTSSITVPGRTINRMGEEVEMITKGRHDPCVGIRAVPIAEAMLAIVLMDHLLRHRAQ
    NADVKTEIPRW
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Inactivation of AroC results in attenuation in humans, and the deletions of both AroC and AroD provide a high level of safety against restoration of pathogenicity by recombination. An aroC/aroD-deleted derivative of S. Typhi strain Ty2 (the parent strain of Ty21a) is designated the strain "CVD 908" (Tacket and Levine, 2007).
  • Related Vaccine(s): Aro-deleted S. Typhi , CVD 908-htrA
5. AroD
  • Gene Name : AroD
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi Ty2
  • NCBI Gene ID : 1070649
  • NCBI Protein GI : 29141695
  • Locus Tag : t1231
  • Genbank Accession : NC_004631.1
  • Protein Accession : NP_805037.1
  • 3D structure: PDB ID : 1QFE
  • Taxonomy ID : 28901
  • Gene Starting Position : 1298692
  • Gene Ending Position : 1299450
  • Gene Strand (Orientation) : -
  • Protein Name : 3-dehydroquinate dehydratase
  • Protein pI : 6.23
  • Protein Weight : 27648.7
  • Protein Length : 252
  • Protein Note : catalyzes the dehydration of 3-dehydroquinate to form 3-dehydroshikimate in aromatic amino acid biosynthesis
  • DNA Sequence : Show Sequence
    >GeneID|1070649 [Salmonella enterica subsp. enterica serovar Typhi Ty2 ] 1298692..1299450
    aggcgttgtgcagaatcattaatacactgcgtagatcatttacggcgatttgccccggcgctgaagcctg
    cttcaccgcgccaaacgtggcggcagagccaaacacttcccctgccagacgtgaaatgacaccctctttc
    gccattgacatagtaattaccggacggtcggcgtaatgttgctgcatctccagcgtggcagtgagtaacg
    ttaatacatcatgcttgctttgcggcataacggcaatcttgggaatatccgcgccgagtgcttgcatttt
    acgtagccgcagaaccatttcctctgcggacggcgtctggtgaaaatcgtggttagacatcacgacataa
    acattatgcgcatgggcataatcgacagtggctttaacgtcagcatcaccggtaaatagctcaagatcga
    tcatatcgaccaggccgctgtcgattgcggcacgattaagcgtgagataatgctgagtggttattgtctg
    ctcgccgccttctttggcgctgcggaaagtaaacagtaacggaatgtcaggcatcgcatcgcggataaca
    cgcgcagcggtaagaacggattgagtcgatgcgatatccataaagtgatccacgcgccactccagaatat
    cgaatgtagcttcgcggtaggccagcgcctccgctttcacgctattgatgtctcttcccatcaacgacac
    gataattttgggcatcccttcgccaatgataagattttttacggtgacggttttcatgc
  • Protein Sequence : Show Sequence
    >gi|29141695|ref|NP_805037.1| 3-dehydroquinate dehydratase [Salmonella enterica subsp. enterica serovar Typhi Ty2 ]
    MKTVTVKNLIIGEGMPKIIVSLMGRDINSVKAEALAYREATFDILEWRVDHFMDIASTQSVLTAARVIRD
    AMPDIPLLFTFRSAKEGGEQTITTQHYLTLNRAAIDSGLVDMIDLELFTGDADVKATVDYAHAHNVYVVM
    SNHDFHQTPSAEEMVLRLRKMQALGADIPKIAVMPQSKHDVLTLLTATLEMQQHYADRPVITMSMAKEGV
    ISRLAGEVFGSAATFGAVKQASAPGQIAVNDLRSVLMILHNA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Inactivation of AroD results in attenuation in humans, and the deletions of both AroC and AroD provide a high level of safety against restoration of pathogenicity by recombination. An aroC/aroD-deleted derivative of S. Typhi strain Ty2 (the parent strain of Ty21a) is designated the strain "CVD 908" (Tacket and Levine, 2007).
  • Related Vaccine(s): Aro-deleted S. Typhi
6. asd
  • Gene Name : asd
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
  • NCBI Protein GI : 267995764
  • Other Database IDs : CDD:180636
    CDD:189205
    CDD:145758
  • Taxonomy ID : 588858
  • Gene Strand (Orientation) : ?
  • Protein Name : aspartate-semialdehyde dehydrogenase
  • Protein Length : 368
  • Protein Note : aspartate-semialdehyde dehydrogenase; Reviewed
  • Protein Sequence : Show Sequence
    >gi|267995764|gb|ACY90649.1| aspartate-semialdehyde dehydrogenase [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]
    MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQFGQAAPTFGDTSTGTLQDAFDLDALKALDII
    VTCQGGDYTNEIYPKLRESGWQGYWIDAASTLRMKDDAIIILDPVNQDVITDGLNNGVKTFVGGNCTVSL
    MLMSLGGLFAHNLVDWVSVATYQAASGGGARHMRELLTQMGQLYGHVADELATPSSAILDIERKVTALTR
    SGELPVDNFGVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTASVIPVDGLCVRVGALRCHSQAFT
    IKLKKEVSIPTVEELLAAHNPWAKVVPNDRDITMRELTPAAVTGTLTTPVGRLRKLNMGPEFLSAFTVGD
    QLLWGAAEPLRRMLRQLA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An asd mutant, alone or in combination with a rfc mutation, is attenuated in mice and induces significant protection from challenge with wild type Salmonella typhimurium (Piao et al., 2010).
  • Related Vaccine(s): Salmonella Typhimurium asd mutant vaccine , Salmonella Typhimurium asd/rfc mutant vaccine
7. atpA
  • Gene Name : atpA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium SL1344
  • NCBI Protein GI : 301160399
  • Locus Tag : STM3867
  • Genbank Accession : AE006468
  • Protein Accession : NP_462766
  • Other Database IDs : CDD:181753
    CDD:199133
    CDD:29998
    CDD:144044
    UniProtKB/TrEMBL: E1WDU3
  • Taxonomy ID : 216597
  • Gene Starting Position : 4076433
  • Gene Ending Position : 4077974
  • Gene Strand (Orientation) : -
  • Protein Name : ATP synthase alpha subunit
  • Protein pI : 5.78
  • Protein Weight : 51045.02
  • Protein Length : 513
  • Protein Note : F0F1 ATP synthase subunit alpha; Validated
  • DNA Sequence : Show Sequence
    >gi|16763390:4076433-4077974 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    GTTACCAGGACTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTATA
    GCCACCGGACTGGTTGATCTCTTGCATCAGCGGAGCGTGATCACGGTCGACGTAAGCCAGCAGTGCGGCT
    TCGAAGCTACCGATTTTCGCCAGTTCGACATCCGCCAGATAACCACGTTCTGCTGCGAACAGAACCAGAG
    ACTGCTGCGCGACGGACATCGGCGCATACTGTTTCTGTTTCAGCAGTTCGGTCACTTTCTGACCGTGGTC
    AAGCTGTTTACGGGTGGCATCGTCAAGGTCGGATGCAAACTGAGAGAACGCCGCCAGTTCACGATACTGC
    GCCAGCGCGGTACGAATACCACCGGACAGTTTTTTCATGATCTTGGTCTGGGCTGCGCCACCTACACGGG
    ATACCGAGATACCCGGGTTAACCGCAGGACGGATACCGGCGTTAAACAGGTTAGATTCCAGGAAGATCTG
    ACCATCGGTAATGGAAATTACGTTGGTCGGAACGAACGCGGAAACGTCACCTGCCTGCGTTTCGATAATC
    GGCAGCGCAGTCAGTGAACCGGTTTTCCCTTTCACTTCACCTTTAGTGAAGGCTTCGACGTAGTCGGCGT
    TAACACGCGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAATACGTCGCCCGGGAATGCTTCACGTCC
    TGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCTGTTTAGACAGGTCATCGTAAATG
    ATCAGCGCATCTTCGCCGCGGTCACGGAAGTATTCGCCCATTGCGCAACCGGCATACGGCGCCAGATATT
    GCAGCGCAGCGGATTCGGACGCGGTCGCCACCACAACAATGGTGTTAGCCAGCGCGCCGTGCTCTTCCAG
    TTTACGCACCACGTTAGAGATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATGCCGGAG
    TCGCGCTGGTTGATGATGGCGTCGATAGCCAGCGCGGTTTTACCGGTCTGACGGTCACCGATGATCAGTT
    CACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAGCCGGTCTGCACCGGCTGATCGAC
    AGACTGACGATCGATAACGCCCGGCGCAATCGCTTCAACGGCAGAGAAGCCGTCATTATCAACCGGACCT
    TTACCGTCAATTGGCGCACCCAGGGTGTTTACCACACGTCCCAGCAGGCCGCGGCCAACCGGAACTTCCA
    GGATACGACCCGTACATTTAACCTTCATGCCTTCGGCAAGGTCAGCGTATGGACCCATGACTACCGCACC
    CACGGAGTCGCGCTCCAGGTTCAGTGCGATAGCGTAACGGTTACCCGGCAGGGAGATCATTTCACCCTGC
    ATACAATCGGCCAGGCCGTGAATGCGGATAACACCGTCACTGACAGAAACAATAGTACCTTCGTTGTGAG
    CTTCGCTCACCACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTG
    CA
  • Protein Sequence : Show Sequence
    >gi|301160399|emb|CBW19925.1| ATP synthase alpha subunit [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
    MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGLADCMQGEMISLPGNRYAIALNLERDS
    VGAVVMGPYADLAEGMKVKCTGRILEVPVGRGLLGRVVNTLGAPIDGKGPVDNDGFSAVEAIAPGVIDRQ
    SVDQPVQTGYKAVDSMIPIGRGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVR
    KLEEHGALANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLLLRRP
    PGREAFPGDVFYLHSRLLERAARVNADYVEAFTKGEVKGKTGSLTALPIIETQAGDVSAFVPTNVISITD
    GQIFLESNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGGIRTALAQYRELAAFSQFASDLDDATRKQ
    LDHGQKVTELLKQKQYAPMSVAQQSLVLFAAERGYLADVELAKIGSFEAALLAYVDRDHAPLMQEINQSG
    GYNDEIEGKLKGILDSFKATQSW
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An atpA mutant is attenuated in mice and induces significant protection from challenge with wild type S. typhimurium (Chaudhuri et al., 2009).
  • Related Vaccine(s): Salmonella Typhimurium atpA mutant vaccine
8. cbiA
  • Gene Name : cbiA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6923268
  • NCBI Protein GI : 205353181
  • Locus Tag : SG2061
  • Genbank Accession : AM933173
  • Protein Accession : YP_002226982
  • Taxonomy ID : 550538
  • Gene Starting Position : 2106950
  • Gene Ending Position : 2108329
  • Gene Strand (Orientation) : -
  • Protein Name : cobyrinic acid a,c-diamide synthase
  • Protein pI : 6.59
  • Protein Weight : 47041.13
  • Protein Length : 459
  • Protein Note : responsible for the amidation of carboxylic groups at position A and C of cobyrinic acid or hydrogenobrynic acid
  • DNA Sequence : Show Sequence
    >gi|205351346:2106950-2108329 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
    GTCATAATACGCGCCTCGCCGCCGCCAGCCAGTGCTGCAACATTTCCGGACGCTGGGCGAAATGGACGTG
    CAGATAGCTGGCAAACGTGTTGCCTGTCTGCCAGCCGCCAGTCCATTCCTGTAACACGCGGCCATCGCGA
    ACCTTACGACAGGCCATAACGGCGGGTGTTTCGGGGATAAAATCTGAGTAGTGAAATTCATGGCCGCGCA
    CTATCTCGCCAAGCGCCGCCAATAGCGTTGGCTGCATAGCCTGCGCCTCACAGTAGCCAAAGCGGGTAAG
    TCGCTTACCCATTTTGCTGTGACCGGGGATAATATTCGCCATCTGATGAATTTCCCCACCGCTATCCTCC
    AGAGTGCTCCCCAGATACATCAGACCGCCACACTCCGCGTAAATCGCCACGCCGCGCTGATGCGCCGCCC
    GCAAATGTTTTAACATCGCCGTATTTGCCGCCAGATCCGCGGCGTATAATTCAGGATATCCACCGCCCAG
    CCAGATCATCTGACAATCAGGCAATGCGCGGTCATGCAGTGGGCTAAAACGCACAATGTTAACGCCGGCA
    CGCTCCAGAAAATCGATGTTATCGGGATAATAAAAGTTAAAGGCCTCATCATCCGCCAGAGCAAGCGTCA
    GCCCTGCCCCCGCTGTGTTATCGGGGCGCTCAGGCCACATTCCCGTCGGAAGCGCTGATAACACGCTCAG
    CGAGAGTAGCGCATCCACATCCACTGTTTGTTCCAGCGAGGCGGCAAAATCATGCCATGATTGCTGATTG
    ACGAGGGATTCTCTGGCGGTAATCAACCCCAGGTGGCGTTCAGGTAATGCGACGCCGTCGCAAGGAGGAA
    CATACCCCAGTACCGGCCGTGAGCAATAATGTTCAATGGCATTTTTGAGGAGCTGATAGTGGGCGTCGCT
    AGTGACGCGATTCACAATCACGCCCGCGAGGTTGAGGGTCGGGTCGAAGTGCTGAAATCCCATAACGGTA
    GCCGCAAGCGACGTTGAAACAGCCTTGCCGTCCACCAGTAGGATGACCGGGCAACCAAGCTGTTTGGCCA
    TCGCGGCTGTGCTGCAGTAATTGGGGTCGACGCCATAGCCGTCATACAGCCCCATGACGCCTTCAATGAC
    AGCGATATCCGCCTGCCGCATTTGTTCGCAAAATAAGGCGTTGAGAACAGGAGGAGGAAGCATGAAACTG
    TCAAGATTACGGGACGCCACGCCACAGATAGCGGTATGCCAGCCGGTATCAAGGTAATCTGGCCCAACTT
    TAAACGGCTGTACGCGCAGCGCGCGTTTTTGCAGCAGCCTCAACAAACCAAGCGTCACCGTGGTTTTGCC
    GCAACCGCTTCCTGTGCCTGCAAGAATAAATGCGTGATGCCTTGCCGCCA
  • Protein Sequence : Show Sequence
    >gi|205353181|ref|YP_002226982.1| cobyrinic acid a,c-diamide synthase [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
    MAARHHAFILAGTGSGCGKTTVTLGLLRLLQKRALRVQPFKVGPDYLDTGWHTAICGVASRNLDSFMLPP
    PVLNALFCEQMRQADIAVIEGVMGLYDGYGVDPNYCSTAAMAKQLGCPVILLVDGKAVSTSLAATVMGFQ
    HFDPTLNLAGVIVNRVTSDAHYQLLKNAIEHYCSRPVLGYVPPCDGVALPERHLGLITARESLVNQQSWH
    DFAASLEQTVDVDALLSLSVLSALPTGMWPERPDNTAGAGLTLALADDEAFNFYYPDNIDFLERAGVNIV
    RFSPLHDRALPDCQMIWLGGGYPELYAADLAANTAMLKHLRAAHQRGVAIYAECGGLMYLGSTLEDSGGE
    IHQMANIIPGHSKMGKRLTRFGYCEAQAMQPTLLAALGEIVRGHEFHYSDFIPETPAVMACRKVRDGRVL
    QEWTGGWQTGNTFASYLHVHFAQRPEMLQHWLAAARRVL
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A cbiA mutant, in combination with a cobS mutation, is attenuated in chickens and induces significant protection from challenge with wild type S. Gallinarum. This mutant was also able to provide some protection against S. Enteritidis (Penha et al., 2010).
  • Related Vaccine(s): Salmonella Gallinarum cbiA/cobS mutant vaccine
9. clpP
  • Gene Name : clpP
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Enteritidis str. P125109
  • NCBI Gene ID : 6950532
  • NCBI Protein GI : 207855928
  • Locus Tag : SEN0430
  • Genbank Accession : AM933172
  • Protein Accession : YP_002242579
  • Taxonomy ID : 550537
  • Gene Starting Position : 482579
  • Gene Ending Position : 483202
  • Gene Strand (Orientation) : ?
  • Protein Name : ATP-dependent Clp protease proteolytic subunit
  • Protein pI : 5.88
  • Protein Weight : 22084.77
  • Protein Length : 207
  • Protein Note : hydrolyzes proteins to small peptides; with the ATPase subunits ClpA or ClpX, ClpP degrades specific substrates
  • DNA Sequence : Show Sequence
    >gi|207855516:482579-483202 Salmonella enterica subsp. enterica serovar Enteritidis str. P125109 chromosome, complete genome
    AATGTCATACAGCGGAGAACGAGATAATTTGGCCCCTCATATGGCGCTGGTGCCGATGGTCATTGAACAG
    ACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGCGTCATATTTCTGACCG
    GCCAGGTCGAAGACCATATGGCTAACCTGATCGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCGGA
    AAAAGATATCTATCTGTACATTAATTCTCCTGGCGGCGTAATTACTGCGGGGATGTCCATCTATGACACC
    ATGCAGTTTATTAAGCCAGACGTCAGCACCATTTGTATGGGACAGGCGGCCTCTATGGGGGCGTTTCTGC
    TGACTGCCGGGGCGAAAGGCAAACGTTTCTGCTTGCCGAACTCTCGCGTCATGATCCACCAGCCGCTGGG
    CGGCTACCAGGGCCAGGCGACGGATATTGAAATTCACGCCCGCGAAATTTTGAAAGTAAAAGGGCGCATG
    AATGAACTTATGGCGCATCATACGGGTCAATCTCTTGAGCAGATTGAACGTGATACTGAGCGCGATCGCT
    TCCTCTCCGCGCCTGAAGCGGTAGAGTACGGTTTGGTTGACTCAATTTTGACCCATCGTAATTG
  • Protein Sequence : Show Sequence
    >gi|207855928|ref|YP_002242579.1| ATP-dependent Clp protease proteolytic subunit [Salmonella enterica subsp. enterica serovar Enteritidis str. P125109]
    MSYSGERDNLAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIFLTGQVEDHMANLIVAQMLFLEAENPE
    KDIYLYINSPGGVITAGMSIYDTMQFIKPDVSTICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLG
    GYQGQATDIEIHAREILKVKGRMNELMAHHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaBA and clpP mutant (CVD 1941) is attenuated in mice after oral immunization. This mutant is also protective, and had a vaccine efficacy of 76% against challenge with wild type Salmonella Enteritidis (Tennant et al., 2011).
  • Related Vaccine(s): Salmonella Enteritidis guaBA/clpP mutant vaccine
10. clpP from S. typhimurium str. LT2
  • Gene Name : clpP from S. typhimurium str. LT2
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1251968
  • NCBI Protein GI : 16763829
  • Locus Tag : STM0448
  • Genbank Accession : AE006468
  • Protein Accession : NP_459444
  • Taxonomy ID : 700720
  • Gene Starting Position : 503209
  • Gene Ending Position : 503832
  • Gene Strand (Orientation) : +
  • Protein Name : ATP-dependent Clp protease proteolytic subunit
  • Protein pI : 5.88
  • Protein Weight : 22084.77
  • Protein Length : 207
  • Protein Note : hydrolyzes proteins to small peptides; with the ATPase subunits ClpA or ClpX, ClpP degrades specific substrates
  • DNA Sequence : Show Sequence
    >gi|16763390:503209-503832 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    AATGTCATACAGCGGAGAACGAGATAATTTGGCCCCTCATATGGCGCTGGTGCCGATGGTCATTGAACAG
    ACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGCGTCATATTTCTGACCG
    GCCAGGTCGAAGACCATATGGCTAACCTGATCGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCGGA
    AAAAGATATCTATCTGTACATTAATTCTCCTGGCGGCGTAATTACTGCGGGGATGTCCATCTATGACACC
    ATGCAGTTTATTAAGCCAGACGTCAGCACCATTTGTATGGGACAGGCGGCCTCTATGGGGGCGTTTCTGC
    TGACTGCCGGGGCGAAAGGCAAACGTTTCTGCTTGCCGAACTCTCGCGTCATGATCCACCAGCCGCTGGG
    CGGCTACCAGGGCCAGGCGACGGATATTGAAATTCACGCCCGCGAAATTTTGAAAGTAAAAGGGCGCATG
    AATGAACTTATGGCGCATCATACGGGTCAATCTCTTGAGCAGATTGAACGTGATACTGAGCGCGATCGCT
    TCCTCTCCGCGCCTGAAGCGGTAGAGTACGGTTTGGTTGACTCAATTTTGACCCATCGTAATTG
  • Protein Sequence : Show Sequence
    >gi|16763829|ref|NP_459444.1| ATP-dependent Clp protease proteolytic subunit [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MSYSGERDNLAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIFLTGQVEDHMANLIVAQMLFLEAENPE
    KDIYLYINSPGGVITAGMSIYDTMQFIKPDVSTICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLG
    GYQGQATDIEIHAREILKVKGRMNELMAHHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A mutation in clpP (CS2007) causes hyperflagellation. These hyperflagellated bacteria are attenuated in mice, and an LD50 for CS2007 could not be determined because mice did not die after inoculation with the highest dose. CS2007 also elicited protection in mice from challenge with wild type S. typhimurium (Matsui et al., 2003).
  • Related Vaccine(s): Salmonella Typhimurium clpP mutant vaccine , Salmonella Typhimurium guaBA/clpP mutant vaccine , Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine
11. cobS
  • Gene Name : cobS
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6923296
  • NCBI Protein GI : 205353166
  • Locus Tag : SG2041
  • Genbank Accession : AM933173
  • Protein Accession : YP_002226967
  • Taxonomy ID : 550538
  • Gene Starting Position : 2092942
  • Gene Ending Position : 2093685
  • Gene Strand (Orientation) : -
  • Protein Name : cobalamin synthase
  • Protein pI : 9.13
  • Protein Weight : 24926.05
  • Protein Length : 247
  • Protein Note : catalyzes the formation of adenosylcobalamin from Ado-cobinamide-GDP and alpha-ribazole
  • DNA Sequence : Show Sequence
    >gi|205351346:2092942-2093685 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
    CTCATAACAGAGCCAGCAGAAAGATCAATTCACCAAGTTCGATCGCCGCGCCCAGCGTATCGCCGGTTTG
    ACCGCCAAGCGTACGTTTAAGCAACTGGCCAAGGATGAAAATCGCCGCGCATGTGACCACCATTGCGGCC
    AACCCTTGCATACCAGGCAGTAATACCGTGGCGACGATTACCGCCAATCCCAGAGTAATGCAGGTTTGCC
    GTCCGCTGACTTTACCGATAAATACATTGCCAAGCCCCTCTTCACGGGCGTAGCGATGACGGTACATCAA
    TAAAACGGCGCTGCCGCGTCCGGCCGCACAGGCCGCCGCCAACGCCGCCAACATTGGCGTTCCACGTAAC
    GCCAGTTCGCTGACCACCAGAATTTTTGCCAGTAGCACAAAAATAAGCGCCAGCCCGCCATGGGTTCCCA
    GACGACTATCACGCATAATCTCCAGCATTCGCTCACGGCGGCGCGCGGAAAAAATACCATCGCAGGTATC
    GGCCAACCCATCCAGGTGAAAACCACCGGTCAGCAACGCCAGCGCCAGAATACAGAACAACGCCGCTAGC
    GGAATACCGCACCAGGGTTGCAGGAGGATGAAAATCAGGCCGCTTACCCCTCCCAGAATCAACCCGATAA
    AGGGAAACATCACGATCCCACGCGAATACTGCTCGAAATCCAGTCCCTGCGACCAGCGTGACGGCACGGG
    CAAGCGGCTAATAAAAGCGAGCATGGCCCAAAACAGCTTACTCA
  • Protein Sequence : Show Sequence
    >gi|205353166|ref|YP_002226967.1| cobalamin synthase [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
    MSKLFWAMLAFISRLPVPSRWSQGLDFEQYSRGIVMFPFIGLILGGVSGLIFILLQPWCGIPLAALFCIL
    ALALLTGGFHLDGLADTCDGIFSARRRERMLEIMRDSRLGTHGGLALIFVLLAKILVVSELALRGTPMLA
    ALAAACAAGRGSAVLLMYRHRYAREEGLGNVFIGKVSGRQTCITLGLAVIVATVLLPGMQGLAAMVVTCA
    AIFILGQLLKRTLGGQTGDTLGAAIELGELIFLLALL
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A cobS mutant, in combination with a cbiA mutation, is attenuated in chickens and induces significant protection from challenge with wild type S. Gallinarum. This mutant was also able to provide some protection against S. Enteritidis (Penha et al., 2010).
  • Related Vaccine(s): Salmonella Gallinarum cbiA/cobS mutant vaccine
12. cpxA
  • Gene Name : cpxA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1255585
  • NCBI Protein GI : 16767324
  • Locus Tag : STM4058
  • Genbank Accession : AE006468
  • Protein Accession : NP_462939
  • Taxonomy ID : 700720
  • Gene Starting Position : 4268375
  • Gene Ending Position : 4269748
  • Gene Strand (Orientation) : -
  • Protein Name : two-component sensor protein
  • Protein pI : 5.57
  • Protein Weight : 49326.93
  • Protein Length : 457
  • Protein Note : part of two-component CpxA/CpxR system; senses envelope stress; upregulates a number of periplasmic folding and trafficking factors
  • DNA Sequence : Show Sequence
    >gi|16763390:4268375-4269748 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    TTTAGGTTCGCTTGTACAGCGGTAGCCACAGCGTAAGCCGCAACCCACCCAGCGGGCTATCGTCAGCCTT
    CACCCAGCCGCGGTGCTGCTGCATGGCGCTTTCGACAATCGCCAGCCCCAGCCCGGTGCCGCCAGACTCG
    CGGTCGCGCGCCTCATCAGTGCGATAGAACGGACGGAAGATCTGCTCGCGGTCTTCAGGGCTGACGCCCG
    GCCCGTCGTCATCGACCGTGATCGTAATACCGTCTTTATCCACCGAGAAGCCGACTTCAATCTTCGTATG
    TGAATAGCGCAGCGCATTACGAACTATATTTTCCAGCGCGCTTTCCAGCGCGTTTGGGTTGCCATAGAGC
    GGCCACGGCCCCGGCGGATAATTTACCGTTAACGACTTGCCCATCTGTTCGGCTTCAAAGGCGGCGTTAT
    CCAGCACTTCGCCCCATAGCTGATTGGCTTTCATCGTTTCGCTGACCAGCGCATTTTTCTGTTGGTTACG
    CGACATCACCAGCAGGTCATTGATCATGCTGTCCAGACGCTGCGCTTCGGTTTCAATACGCTCCAGCTCT
    TTGCTTTCGCCGCCACGACGACGCAGCAGCGCGGTACCCAGTTGCAGGCGCGTAAGGGGCGTTCGTAGCT
    CATGGGAGATGTCTGACAGCAAACGCTGCTGCGAGGTCATCATCCGTTCCAGCGCCGTCACCATCTGGTT
    AAAACTGGCGCCAGCGGCGAGGAACTCCTGCGGACCCGCCTCCAGCTCCGGATGCTGACGCAGGTTGCCT
    TGCGCCACTTCATCAGCCGCGTTTTTCAACTTACGCGCCGGTTTCGCCAGACTCCATGCCAGCCATAGCA
    AGAGCGGCGAACTGACCAGCATCGTGACAATGAGCAACAGAAGCGGGCGGTCAAACAGCAGATTAATAAA
    ATCGGATTGCGAACTGCTGGCCGGTCGAATCAAGTAAAGCTGGTAATTATCCTCTCCGTCGCGAACGGAG
    AACGGCCCCACCATCTCTACGCGGCCATATTTTTTCTTCTGCGGATGATCGGCGTTATCCGCCTGACCAA
    TGAAGTTACGAATGATCTGCATTTCGCTGCGTTCAGCGCCGATCACGCGTCCTTCAGAGGTCACCAGTAA
    TAACCGCTGTCCAGGCGGCGCCCACTTATCGATCGCGCGGAACAGGCGACGCCACCACATCAGGTCGTTG
    GGCGGATCGTTCGCAAGTTCAGCTTCTACATGTTGCTCTATCATCAATCCCTGGCGCTGTTCGCTGTCCA
    GCAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGCAACATCAGGACCAGCATTAGCACCAGCGCCAGCGT
    CAACCAGAAGATGGCGAAGATGCGCGCGGTTAAACTTCCTATCA
  • Protein Sequence : Show Sequence
    >gi|16767324|ref|NP_462939.1| two-component sensor protein [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQRQGLMIEQHVEAELANDPPNDLMWWRR
    LFRAIDKWAPPGQRLLLVTSEGRVIGAERSEMQIIRNFIGQADNADHPQKKKYGRVEMVGPFSVRDGEDN
    YQLYLIRPASSSQSDFINLLFDRPLLLLIVTMLVSSPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPE
    LEAGPQEFLAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRGGESKELERIETE
    AQRLDSMINDLLVMSRNQQKNALVSETMKANQLWGEVLDNAAFEAEQMGKSLTVNYPPGPWPLYGNPNAL
    ESALENIVRNALRYSHTKIEVGFSVDKDGITITVDDDGPGVSPEDREQIFRPFYRTDEARDRESGGTGLG
    LAIVESAMQQHRGWVKADDSPLGGLRLTLWLPLYKRT
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A gyrA-cpxA-rpoB Salmonella Typhimurium mutant was attenuated and protected against challenge in pigs (Roesler et al., 2004).
  • Related Vaccine(s): Salmonella Typhimurium gyrA/cpxA/rpoB mutant vaccine
13. cpxR
  • Gene Name : cpxR
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6923307
  • NCBI Protein GI : 205354363
  • Locus Tag : SG3362
  • Genbank Accession : AM933173
  • Protein Accession : YP_002228164
  • Taxonomy ID : 550538
  • Gene Starting Position : 3515980
  • Gene Ending Position : 3516678
  • Gene Strand (Orientation) : ?
  • Protein Name : DNA-binding transcriptional regulator CpxR
  • Protein pI : 5.38
  • Protein Weight : 25082.91
  • Protein Length : 232
  • Protein Note : response regulator in two-component regulatory system with CpxA; part of the envelope stress response system
  • DNA Sequence : Show Sequence
    >gi|205351346:3515980-3516678 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
    AATGAATAAAATCCTGTTAGTTGATGATGACCGAGAGCTGACTTCCCTGTTAAAAGAGCTCCTCGAAATG
    GAAGGTTTTAATGTCCTGGTGGCCCACGACGGCGAGCAGGCGCTTGAGCTTTTGGATGACAGCATCGATT
    TACTTTTGCTTGACGTCATGATGCCGAAGAAAAACGGTATCGATACGTTGAAAGCGCTTCGCCAGACACA
    CCAGACGCCTGTCATTATGCTGACCGCGCGCGGCAGCGAGCTGGATCGCGTTCTCGGCCTTGAGCTGGGC
    GCGGACGACTATTTGCCCAAACCGTTTAACGACCGCGAGCTGGTAGCGCGCATCAGGGCTATTTTGCGCC
    GTTCCCACTGGAGCGAACAGCAGCAGAGCAGCGACAACGGCTCGCCGACGCTGGAAGTCGATGCGCTAAG
    CCTTAATCCGGGTCGCCAGGAAGCCAGTTTCGATGGCCAAACGCTGGAGCTGACCGGTACGGAATTCACC
    CTGCTCTATTTGCTGGCGCAACACCTCGGCCAGGTGGTTTCCCGTGAACATTTAAGCCAGGAAGTGCTGG
    GTAAGCGCCTGACGCCGTTCGATCGCGCCATTGATATGCATATTTCTAACCTGCGCCGCAAACTGCCGGA
    ACGCAAAGACGGTCACCCGTGGTTTAAAACATTGCGTGGTCGCGGCTATCTGATGGTTTCCGCTTCATG
  • Protein Sequence : Show Sequence
    >gi|205354363|ref|YP_002228164.1| DNA-binding transcriptional regulator CpxR [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
    MNKILLVDDDRELTSLLKELLEMEGFNVLVAHDGEQALELLDDSIDLLLLDVMMPKKNGIDTLKALRQTH
    QTPVIMLTARGSELDRVLGLELGADDYLPKPFNDRELVARIRAILRRSHWSEQQQSSDNGSPTLEVDALS
    LNPGRQEASFDGQTLELTGTEFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPE
    RKDGHPWFKTLRGRGYLMVSAS
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A cpxR mutant, in combination with a lon mutation, is highly attenuated in mice and induces significant protection from challenge with wild type S. gallinarum (Matsuda et al., 2010).
  • Related Vaccine(s): Salmonella Gallinarum cpxR/Ion mutant vaccine
14. crp
  • Gene Name : crp
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum
  • NCBI Nucleotide GI : 46811320
  • NCBI Protein GI : 46811321
  • Protein Accession : AAT01929.1
  • Taxonomy ID : 594
  • Gene Strand (Orientation) : ?
  • Protein Name : cAMP receptor protein
  • Protein Note : cyclic AMP receptor protein; CAP; catabolite gene activator protein
  • DNA Sequence : Show Sequence
    >gi|46811320|gb|AY594269.1| Salmonella enterica subsp. enterica serovar Gallinarum cAMP receptor protein gene, complete cds
    CAATCTATTTTCGTAGCCCCCTTCCCAGGTAGCGGGAAGTATATTTTTGCAACCCCAGAGACAGTGCCGT
    TTTCTGGCTCTGGAGACAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACC
    CGACTCTTGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCGTCAAAGAGCACGCTGATTCACCA
    GGGTGAAAAAGCAGAAACGCTGTACTACATCGTTAAAGGCTCCGTGGCAGTGCTGATCAAAGATGAAGAA
    GGGAAAGAAATGATCCTTTCTTATCTGAATCAGGGTGATTTTATTGGTGAACTGGGCCTGTTTGAAGAAG
    GCCAGGAACGCAGCGCCTGGGTACGTGCGAAAACCGCATGTGAGGTCGCTGAAATTTCCTACAAAAAATT
    TCGCCAATTAATCCAGGTCAACCCGGATATTCTGATGCTCCTCTCTTCCCAGATGGCTCGTCGCTTACAA
    GTCACCTCTGAAAAAGTAGGTAACCTCGCCTTCCTTGACGTCACCGGGCGTATCGCTCAGACGCTGCTGA
    ATCTGGCGAAACAGCCCGATGCCATGACGCACCCGGATGGGATGCAGATCAAAATCACCCGTCAGGAAAT
    CGGCCAGATCGTCGGCTGCTCCCGCGAAACCGTTGGTCGTATTTTGAAAATGCTGGAAGATCAAAACCTG
    ATCTCCGCGCATGGCAAGACCATCGTCGTCTACGGCACCCGTTAATTCCGTCAGAATGGCG
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A crp mutant is attenuated in mice and induces significant protection from challenge with wild type S. gallinarum (Rosu et al., 2007).
  • Related Vaccine(s): Salmonella Gallinarum crp mutant vaccine
15. crp
  • Gene Name : crp
  • Taxonomy ID : 99287
16. crp from S. Typhimurium
  • Gene Name : crp from S. Typhimurium
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1254989
  • NCBI Protein GI : 16766754
  • Locus Tag : STM3466
  • Genbank Accession : AE006468
  • Protein Accession : NP_462369
  • Taxonomy ID : 99287
  • Gene Starting Position : 3615644
  • Gene Ending Position : 3616276
  • Gene Strand (Orientation) : +
  • Protein Name : cAMP-regulatory protein
  • Protein pI : 8.46
  • Protein Weight : 22267.2
  • Protein Length : 210
  • Protein Note : complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons
  • DNA Sequence : Show Sequence
    >gi|16763390:3615644-3616276 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    CATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTTGAATGGTTCTTGTCTCATTGCCACATTCATAAG
    TACCCGTCAAAGAGCACGCTGATTCACCAGGGTGAAAAAGCAGAAACGCTGTACTACATCGTTAAAGGCT
    CCGTGGCAGTGCTGATCAAAGATGAAGAAGGGAAAGAAATGATCCTTTCTTATCTGAATCAGGGTGATTT
    TATTGGTGAACTGGGCCTGTTTGAAGAAGGCCAGGAACGCAGCGCCTGGGTACGTGCGAAAACCGCATGT
    GAGGTCGCTGAAATTTCCTACAAAAAATTTCGCCAATTAATCCAGGTCAACCCGGATATTCTGATGCGCC
    TCTCTTCCCAGATGGCTCGTCGCTTACAAGTCACCTCTGAAAAAGTAGGTAACCTCGCCTTCCTTGACGT
    CACCGGGCGTATCGCTCAGACGCTGCTGAATCTGGCGAAACAGCCCGATGCCATGACGCACCCGGATGGG
    ATGCAGATCAAAATCACTCGTCAGGAAATCGGCCAGATCGTCGGCTGCTCCCGCGAAACCGTTGGTCGTA
    TTTTGAAAATGCTGGAAGATCAAAACCTGATCTCCGCGCATGGCAAGACCATCGTCGTCTACGGCACCCG
    TTA
  • Protein Sequence : Show Sequence
    >gi|16766754|ref|NP_462369.1| cAMP-regulatory protein [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDF
    IGELGLFEEGQERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSSQMARRLQVTSEKVGNLAFLDV
    TGRIAQTLLNLAKQPDAMTHPDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A cya and crp mutant is attenuated in mice. Thirty days after immunization, mice were protected from oral challenge with wild type S. typhimurium (Curtiss and Kelly, 1987).
  • Related Vaccine(s): Salmonella Typhimurium cya/crp mutant vaccine
17. cya
  • Gene Name : cya
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • NCBI Protein GI : 581756
  • Other Database IDs : CDD:193111
    GOA:P0A1A7
    InterPro: IPR000274
    UniProtKB/UniProt: P0A1A7
  • Taxonomy ID : 90371
  • Gene Strand (Orientation) : ?
  • Protein Name : adenylate cyclase
  • Protein Length : 29
  • Protein Note : Adenylate cyclase NT domain; pfam12633
  • Protein Sequence : Show Sequence
    >gi|581756|emb|CAA39304.1| adenylate cyclase [Salmonella enterica subsp. enterica serovar Typhimurium]
    MYLYIETLKQRLDAINQLRVDRALAAMGP
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A cya and crp mutant is attenuated in mice. Thirty days after immunization, mice were protected from oral challenge with wild type S. typhimurium (Curtiss and Kelly, 1987).
  • Related Vaccine(s): Salmonella Typhimurium cya/crp mutant vaccine
18. DnaJ
  • Gene Name : DnaJ
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. CT18
  • VO ID : VO_0011268
  • NCBI Gene ID : 1246524
  • NCBI Protein GI : 16759006
  • Locus Tag : STY0013
  • Genbank Accession : AL627265
  • Protein Accession : NP_454623
  • Taxonomy ID : 220341
  • Gene Starting Position : 13595
  • Gene Ending Position : 14734
  • Gene Strand (Orientation) : +
  • Protein Name : chaperone protein DnaJ
  • Protein pI : 8.26
  • Protein Weight : 38831.98
  • Protein Length : 379
  • Protein Note : chaperone Hsp40; co-chaperone with DnaK; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion
  • DNA Sequence : Show Sequence
    >gi|16758993:13595-14734 Salmonella enterica subsp. enterica serovar Typhi str. CT18 chromosome, complete genome
    GATGGCGAAAAGAGATTACTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAAAAAG
    GCGTATAAGCGCCTGGCCATGAAATATCATCCGGACCGCAATCAGGGTGATAAAGAGGCCGAAGCTAAGT
    TTAAAGAGATTAAAGAAGCCTACGAAGTGCTGACCGATGCGCAAAAACGCGCAGCCTACGATCAGTATGG
    TCACGCCGCGTTTGAACAAGGCGGGATGGGCGGCGGATTTGGCGGCGGCTTTAATGGCGGCGCTGATTTC
    AGTGATATCTTTGGTGACGTTTTTGGCGATATCTTTGGCGGCGGGCGTGGTCGCCAACGTGCGGCGCGTG
    GGGCTGATTTGCGTTATAACATGGATCTCACCCTGGAAGAAGCGGTGCGTGGCGTGACCAAAGAGATCCG
    TATTCCGACGCTGGAGGAGTGCGACGTTTGCCACGGCAGCGGCGCGAAAGCTGGTACGCAACCGCAAACC
    TGTCCGACCTGTCATGGTTCTGGTCAGGTACAGATGCGCCAGGGATTCTTTGCTGTACAGCAGACCTGCC
    CACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCCATAAATGTCACGGTCATGGGCGTGTTGA
    AAAGAGTAAAACTCTGTCCGTTAAAATCCCGGCGGGCGTGGATACCGGCGATCGTATTCGTCTGGCAGGC
    GAGGGCGAAGCGGGCGAGCATGGCGCACCGGCAGGCGACTTGTACGTTCAGGTCCAGGTGAAACAACACC
    CTATTTTCGAGCGTGAAGGCAATAATCTTTATTGCGAAGTGCCGATCAACTTTGCGATGGCGGCGCTCGG
    CGGTGAAATTGAAGTGCCGACGTTAGATGGTCGCGTGATGCTGAAAGTACCGAGCGAAACACAAACGGGC
    AAGCTGTTCCGTATGCGCGGCAAAGGCGTGAAGTCCGTACGCGGTGGCGCGCAGGGCGATTTGCTGTGCC
    GTGTGGTAGTTGAAACGCCGGTCGGTCTGAGCGAAAAACAGAAGCAATTGCTAAAAGATCTTCAGGAAAG
    TTTTGGCGGCCCGACGGGAGAGAAAAACAGCCCGCGTTCAAAAAGCTTCTTTGACGGCGTGAAAAAATTC
    TTTGACGATTTGACTCGCTA
  • Protein Sequence : Show Sequence
    >gi|16759006|ref|NP_454623.1| chaperone protein DnaJ [Salmonella enterica subsp. enterica serovar Typhi str. CT18]
    MAKRDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAEAKFKEIKEAYEVLTDAQKRAAYDQYG
    HAAFEQGGMGGGFGGGFNGGADFSDIFGDVFGDIFGGGRGRQRAARGADLRYNMDLTLEEAVRGVTKEIR
    IPTLEECDVCHGSGAKAGTQPQTCPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCHKCHGHGRVE
    KSKTLSVKIPAGVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAALG
    GEIEVPTLDGRVMLKVPSETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLSEKQKQLLKDLQES
    FGGPTGEKNSPRSKSFFDGVKKFFDDLTR
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Immunisation of mice with DnaJ derived from Salmonella enterica subsp. enterica serovar Typhi str. CT18 was found to provide 70% protection against lethal challenge by S. Typhimurium indicating the possible use of DnaJ as vaccine candidate against typhoid (Sagi et al., 2006).
  • Related Vaccine(s): S. typhi DnaJ Protein Vaccine
19. dsd
  • Gene Name : dsd
20. fliC
  • Gene Name : fliC
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Enteritidis
  • NCBI Protein GI : 119351647
  • Other Database IDs : CDD:181201
  • Taxonomy ID : 149539
  • Gene Strand (Orientation) : ?
  • Protein Name : FliC
  • Protein Length : 157
  • Protein Note : PT1
  • Protein Sequence : Show Sequence
    >gi|119351647|gb|ABL63539.1| FliC [Salmonella enterica subsp. enterica serovar Enteritidis]
    GETITIDLQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKNVTGYDTYAAGADKYRVDINSGAVVTDAAAP
    DKVYVNAANGQLTTDDAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGDD
    GNGKVSTTINGEKVTLT
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A fliC mutant, in combination with a guaB mutant, is attenuated in mice and chickens and induces significant protection from challenge with wild type S. enterica (Adriaensen et al., 2007).
  • Related Vaccine(s): Salmonella enterica fliC/guaB mutant vaccine
21. fliD
  • Gene Name : fliD
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1253481
  • NCBI Protein GI : 16765298
  • Locus Tag : STM1960
  • Genbank Accession : AE006468
  • Protein Accession : NP_460913
  • Taxonomy ID : 700720
  • Gene Starting Position : 2049401
  • Gene Ending Position : 2050804
  • Gene Strand (Orientation) : +
  • Protein Name : flagellar capping protein
  • Protein pI : 5.02
  • Protein Weight : 47254.05
  • Protein Length : 467
  • Protein Note : involved in flagellin assembly
  • DNA Sequence : Show Sequence
    >gi|16763390:2049401-2050804 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    CATGGCTTCAATTTCATCATTAGGTGTGGGATCAAACTTACCGTTAGACCAGTTGTTGACAGACCTGACA
    AAGAACGAAAAAGGACGCTTAACGCCAATTACCAAACAGCAGAGCGCGAATTCGGCAAAGCTAACCGCCT
    ATGGCACATTGAAAAGCGCATTAGAAAAATTCCAGACGGCAAATACCGCGTTAAATAAAGCGGATTTATT
    TAAGTCTACCGTGGCGTCCAGCACCACTGAAGATCTCAAAGTCAGTACTACCGCTGGCGCTGCCGCAGGG
    ACTTATAAGATTAACGTAACCCAGCTTGCCGCCGCACAGTCGCTGGCGACAAAAACCACCTTCGCGACCA
    CCAAAGAGCAGTTGGGCGATACGTCGGTCACGTCCCGGACAATTAAAATTGAACAGCCGGGACGTAAAGA
    GCCGCTGGAAATTAAGCTGGATAAAGGCGACACCTCCATGGAGGCGATCCGTGACGCCATCAATGACGCC
    GACAGCGGTATCGCCGCCAGTATCGTTAAGGTCAAAGAGAACGAATTCCAGTTGGTGCTTACCGCCAATA
    GCGGTACCGACAATACGATGAAGATCACGGTGGAAGGCGATACAAAACTTAACGATCTACTCGCTTATGA
    CAGCACCACCAATACCGGCAATATGCAAGAGCTGGTGAAAGCAGAAAACGCGAAGCTGAACGTAAACGGC
    ATCGACATTGAGCGTCAGAGCAATACCGTAACCGACGCCCCTCAGGGAATTACGCTCACCCTGACCAAGA
    AAGTGACCGACGCGACCGTGACGGTAACGAAAGATGATACCAAGGCGAAAGAGGCGATTAAATCCTGGGT
    GGATGCCTATAACTCGCTGGTGGATACCTTTAGCTCGTTAACCAAATATACCGCCGTTGAGCCGGGCGAA
    GAAGCCAGCGATAAAAACGGCGCGCTGTTAGGCGATAGTGTGGTTCGTACTATCCAGACCGGGATTCGGG
    CACAATTTGCCAATAGCGGCAGTAATTCTGCGTTCAAAACAATGGCGGAAATTGGCATCACCCAGGATGG
    GACTTCCGGCAAACTGAAGATTGATGATGATAAGCTGACCAAAGTACTGAAAGATAACACAGCCGCAGCG
    CGTGAGCTGCTGGTAGGCGATGGTAAAGAAACGGGTATCACCACCAAAATTGCCACCGAAGTGAAAAGTT
    ATCTGGCGGATGACGGCATTATTGATAATGCGCAGGACAACGTTAACGCCACGCTGAAAAGCCTGACAAA
    ACAGTACCTGTCCGTTAGCAACAGCATCGATGAAACCGTTGCCCGTTACAAGGCCCAGTTTACCCAACTG
    GATACCATGATGAGTAAGCTGAATAACACCAGTAGTTATTTGACCCAGCAATTTACAGCTATGAACAAGT
    CCTG
  • Protein Sequence : Show Sequence
    >gi|16765298|ref|NP_460913.1| flagellar capping protein [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MASISSLGVGSNLPLDQLLTDLTKNEKGRLTPITKQQSANSAKLTAYGTLKSALEKFQTANTALNKADLF
    KSTVASSTTEDLKVSTTAGAAAGTYKINVTQLAAAQSLATKTTFATTKEQLGDTSVTSRTIKIEQPGRKE
    PLEIKLDKGDTSMEAIRDAINDADSGIAASIVKVKENEFQLVLTANSGTDNTMKITVEGDTKLNDLLAYD
    STTNTGNMQELVKAENAKLNVNGIDIERQSNTVTDAPQGITLTLTKKVTDATVTVTKDDTKAKEAIKSWV
    DAYNSLVDTFSSLTKYTAVEPGEEASDKNGALLGDSVVRTIQTGIRAQFANSGSNSAFKTMAEIGITQDG
    TSGKLKIDDDKLTKVLKDNTAAARELLVGDGKETGITTKIATEVKSYLADDGIIDNAQDNVNATLKSLTK
    QYLSVSNSIDETVARYKAQFTQLDTMMSKLNNTSSYLTQQFTAMNKS
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaBA, clpP, and fliD mutant (CVD 1923) is attenuated in mice after oral immunization. This mutant is also protective, with a vaccine efficacy of 80% in mice against wild type challenge from Salmonella Typhimurium (Tennant et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine
22. Frag C
  • Gene Name : Frag C
  • Sequence Strain (Species/Organism) : Escherichia coli O157:H7 EDL933
  • NCBI Gene ID : 958167
  • NCBI Protein GI : 15803220
  • Locus Tag : Z4009
  • Genbank Accession : NC_002655.2
  • Protein Accession : NP_289252.1
  • Taxonomy ID : 562
  • Gene Starting Position : 3617920
  • Gene Ending Position : 3618570
  • Gene Strand (Orientation) : +
  • Protein Name : PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components; frag
  • Protein pI : 4.79
  • Protein Weight : 22425.1
  • Protein Length : 216
  • Protein Sequence : Show Sequence
    >gi|15803220|ref|NP_289252.1| PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components; frag [Escherichia coli O157:H7 EDL933 ]
    MEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVAVLFQSGRDTIDTV
    LKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLALICSFPLLSPFLGPGAVIAQVIGVL
    IGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEARZDTVRVGVPSVLVSRFLTGAPTVLIAWFV
    SGFIYQ
  • Related Vaccine(s): CVD 915
23. fsd
  • Gene Name : fsd
  • Molecule Role : Defense factor
24. galE
  • Gene Name : galE
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. Ty2
  • NCBI Gene ID : 1067209
  • NCBI Protein GI : 29142526
  • Locus Tag : t2111
  • Genbank Accession : AE014613
  • Protein Accession : NP_805868
  • Taxonomy ID : 209261
  • Gene Starting Position : 2174895
  • Gene Ending Position : 2175911
  • Gene Strand (Orientation) : +
  • Protein Name : UDP-galactose-4-epimerase
  • Protein pI : 6.18
  • Protein Weight : 34020.42
  • Protein Length : 338
  • Protein Note : corresponds to STY0809 from Accession AL513382: Salmonella typhi CT18
  • DNA Sequence : Show Sequence
    >gi|29140543:2174895-2175911 Salmonella enterica subsp. enterica serovar Typhi str. Ty2 chromosome, complete genome
    TATGAGAGTATTGGTTACAGGTGGTAGCGGTTACATTGGAAGTCATACCTGCGTACAATTGCTGCAAAAT
    GGTCATGACGTCGTCATCCTCGATAACCTCTGCAACAGCAAGCGCAGCGTGCTGCCCGTTATTGAACGTC
    TGGGCGGTAAGCACCCGACCTTTGTCGAAGGCGATATTCGCAACGAAGCGCTTATAACCGAAATTCTGCA
    CGATCACGCGATTGATACCGTGATTCACTTTGCCGGCCTGAAAGCCGTCGGCGAATCGGTCGCCAAGCCG
    CTGGAGTACTATGACAACAACGTCAACGGTACGCTGCGGTTGGTCAGCGCCATGCGTGCGGCCAACGTTA
    AAAACCTTATTTTTAGCTCCTCTGCCACCGTTTATGGCGATCAGCCCAAAATTCCTTACGTCGAAAGTTT
    TCCTACCGGCACGCCGCAAAGCCCCTACGGCAAAAGTAAATTGATGGTAGAACAGATCCTCACCGATCTG
    CAAAAAGCCCAGCCGGAGTGGAGTATTGCGCTGCTGCGTTATTTCAATCCGGTCGGCGCGCACCCGTCGG
    GCGACATGGGAGAAGATCCGCAAGGTATTCCGAATAACCTGATGCCCTATATCGCCCAGGTCGCCGTGGG
    TCGTCGCGAATCGCTCGCCGTTTTCGGCAACGATTACCCGACCGAGGATGGCACCGGCGTACGCGATTAC
    ATTCACGTTATGGACTTAGCCGACGGGCACGTCGTGGCGATGGAAAAACTGGCGGACAAATCCGGCGTAC
    ATATTTATAACCTCGGCGCGGGTGTCGGCAGTAGCGTCCTGGATGTGGTCAACGCCTTTAGTAAAGCCTG
    CGGTAAACCCATTAATTACCACTTCGCGCCGCGTCGCGACGGCGATCTCCCGGCGTATTGGGCGGATGCC
    AGCAAAGCCGATCGCGAGCTGAACTGGCGCGTCACCCGCACGCTTGACGAAATGGCGCAGGACACTTGGC
    ACTGGCAGTCACGCCATCCGCAGGGATACTCAGATTA
  • Protein Sequence : Show Sequence
    >gi|29142526|ref|NP_805868.1| UDP-galactose-4-epimerase [Salmonella enterica subsp. enterica serovar Typhi str. Ty2]
    MRVLVTGGSGYIGSHTCVQLLQNGHDVVILDNLCNSKRSVLPVIERLGGKHPTFVEGDIRNEALITEILH
    DHAIDTVIHFAGLKAVGESVAKPLEYYDNNVNGTLRLVSAMRAANVKNLIFSSSATVYGDQPKIPYVESF
    PTGTPQSPYGKSKLMVEQILTDLQKAQPEWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVG
    RRESLAVFGNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLADKSGVHIYNLGAGVGSSVLDVVNAFSKAC
    GKPINYHFAPRRDGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQGYSD
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A gal E mutant in strain Ty2 was isolated, attenuated, and protected mice against lethal challenge (Germanier and Füer, 1975).
  • Related Vaccine(s): Salmonella Typhi galE mutant vaccine
25. GroEL
  • Gene Name : GroEL
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. Ty2
  • VO ID : VO_0010998
  • NCBI Gene ID : 1068458
  • NCBI Protein GI : 29144631
  • Locus Tag : t4382
  • Genbank Accession : AE014613
  • Protein Accession : NP_807973
  • Taxonomy ID : 209261
  • Gene Starting Position : 4534506
  • Gene Ending Position : 4536152
  • Gene Strand (Orientation) : +
  • Protein Name : chaperonin GroEL
  • Protein pI : 4.57
  • Protein Weight : 51532.68
  • Protein Length : 548
  • Protein Note : 60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is essential for growth
  • DNA Sequence : Show Sequence
    >gi|29140543:4534506-4536152 Salmonella enterica subsp. enterica serovar Typhi str. Ty2, complete genome
    AATGGCAGCTAAAGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTG
    GCAGATGCAGTGAAAGTAACCCTCGGTCCGAAAGGCCGTAACGTGGTTCTGGATAAATCTTTCGGTGCGC
    CGACTATCACTAAAGATGGTGTTTCCGTAGCGCGTGAAATCGAGCTGGAAGACAAGTTTGAAAACATGGG
    CGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCGAACGACGCTGCAGGCGACGGCACCACCACCGCGACC
    GTACTGGCGCAGTCCATCATTACCGAAGGCCTGAAAGCCGTTGCTGCGGGCATGAACCCGATGGACCTGA
    AACGTGGTATCGACAAAGCGGTTGCTGCTGCGGTTGAAGAGCTGAAGGCGCTGTCCGTACCGTGCTCCGA
    CTCTAAAGCGATTGCTCAGGTAGGTACTATTTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCG
    GAAGCGATGGATAAAGTCGGTAAAGAAGGCGTCATCACTGTTGAAGACGGTACCGGTCTGCAGGACGAAC
    TGGACGTGGTTGAAGGTATGCAGTTTGACCGCGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAAC
    TGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGATAAGAAAATCTCCAACATCCGCGAAATG
    CTGCCGGTTCTGGAAGCCGTTGCAAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTTGAAGGCG
    AAGCGCTGGCTACCCTGGTAGTGAACACCATGCGTGGCATCGTGAAAGTGGCTGCGGTTAAAGCACCGGG
    CTTCGGCGATCGTCGTAAGGCGATGCTGCAGGATATCGCTACCCTGACCGGCGGTACCGTAATCTCTGAA
    GAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCGAAACGTGTTGTGATCAACA
    AAGACACCACCACCATCATTGATGGCGTGGGTGAAGAAGCTGCCATCCAGGGCCGTGTTGCTCAGATCCG
    TCAGCAGATTGAAGAAGCGACCTCCGACTACGATCGTGAAAAACTGCAGGAGCGCGTAGCGAAACTGGCA
    GGCGGCGTTGCGGTAATCAAAGTTGGCGCTGCGACCGAAGTTGAAATGAAAGAGAAGAAAGCCCGCGTTG
    AAGATGCCCTGCACGCGACCCGTGCTGCGGTAGAAGAAGGCGTGGTTGCTGGTGGTGGCGTTGCGCTGAT
    CCGCGTTGCTTCTAAAATTGCTGACCTGAAAGGCCAGAACGAAGACCAGAACGTGGGTATCAAAGTTGCG
    CTGCGCGCAATGGAAGCTCCGCTGCGTCAGATCGTGCTGAACTGCGGCGAAGAGCCGTCTGTTGTCGCTA
    ACACCGTTAAAGGCGGCGACGGTAACTACGGTTACAACGCAGCAACTGAAGAATACGGCAACATGATCGA
    TATGGGTATCTTGGACCCAACCAAAGTTACCCGTTCTGCGCTGCAATACGCGGCTTCTGTGGCTGGTCTG
    ATGATCACTACCGAGTGCATGGTGACCGACCTGCCGAAAAGCGATGCTCCTGATTTAGGCGCTGCTGGCG
    GCATGGGTGGTATGGGTGGTATGGGCGGCATGATGTA
  • Protein Sequence : Show Sequence
    >gi|29144631|ref|NP_807973.1| chaperonin GroEL [Salmonella enterica subsp. enterica serovar Typhi str. Ty2]
    MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMG
    AQMVKEVASKANDAAGDGTTTATVLAQSIITEGLKAVAAGMNPMDLKRGIDKAVAAAVEELKALSVPCSD
    SKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPET
    GAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPG
    FGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIR
    QQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALI
    RVASKIADLKGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMID
    MGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKSDAPDLGAAGGMGGMGGMGGMM
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Researchers evaluated the immunogenicity and protective efficacy of GroEL of Salmonella enterica serovar Typhi against lethal infection by S. typhi Ty2 in mice with or without adjuvants. Immunization of mice with GroEL-alone, GroEL + alum, and GroEL + CFA provided 70, 50 and 80% protection, respectively, against lethal challenge by S. typhi in mice. The present study shows that GroEL forms an ideal candidate molecule to develop a recombinant protein based vaccine against human typhoid (Bansal et al., 2010).
  • Related Vaccine(s): S. typhi GroEL Protein Vaccine
26. guaA
  • Gene Name : guaA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Enteritidis str. P125109
  • NCBI Gene ID : 6948075
  • NCBI Protein GI : 207857920
  • Locus Tag : SEN2490
  • Genbank Accession : AM933172
  • Protein Accession : YP_002244571
  • Taxonomy ID : 550537
  • Gene Starting Position : 2623837
  • Gene Ending Position : 2625414
  • Gene Strand (Orientation) : -
  • Protein Name : GMP synthase
  • Protein pI : 5.06
  • Protein Weight : 53428.72
  • Protein Length : 525
  • Protein Note : contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway
  • DNA Sequence : Show Sequence
    >gi|207855516:2623837-2625414 Salmonella enterica subsp. enterica serovar Enteritidis str. P125109 chromosome, complete genome
    CTTATTCCCACTCAATGGTAGCTGGTGGTTTACCGCTGATGTCATACACCACACGGGAAATCCCGTTGAC
    TTCATTGATGATGCGGTTGGAAACACGACCCAGGAAATCATACGGCAGATGTGCCCAGTGTGCGGTCATA
    AAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAATCGTACTTACGACCATCGCCCATCACGCCTA
    CGGAACGGACCGGCAGGAAGACGGTAAACGCCTGACTGACTTTGTCGTACAGATCCGCTTTGCGCAGCTC
    TTCAATGAAGATAGCGTCCGCGCGACGCAGCAGGTCGCAGTACTCTTTCTTCACTTCGCCCAGTACGCGC
    ACGCCGAGGCCCGGCCCCGGGAACGGATGGCGATACAGCATGTCGTACGGCAGGCCCAGCTCCAGACCAA
    TCTTACGCACTTCGTCTTTGAACAGCTCTTTCAGCGGTTCAACCAGCCCCATCTTCATCTCTTTCGGCAG
    GCCGCCGACATTGTGGTGAGATTTGATGACGTGCGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACG
    TCAGGGTAGATGGTGCCCTGCGCCAGCCATTTCACGTCTTCCAGTTTCAGCGCTTCTTCGTCGAACACTT
    CAACGAAAACGCGGCCGATGATCTTACGCTTGGCTTCCGGATCGTTCTCACCAGCCAGCGCGGACAGGAA
    GCGATCTTCTGCCGGTACGTGAACAATGTTCAGACCAAAATGGTCGCCAAACATGTCCATCACCTGCTCG
    GCTTCGTTCAGGCGCAACAGGCCGTTGTCGACGAAAACACAGGTCAGATTTTTACCGATCGCGCGGTGCA
    GCAGCATGGCGGTGACGGAAGAATCCACGCCGCCGGAGAGACCGAGGATCACTTTATCGTCGCCTACCTG
    CTCGCGAATGCGCGCCACGGCGTCATCGATGATCTTCGCCGGCGTCCACAACGCTTCACACTGGCAGATA
    TCGCGTACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTGTGAGTCACTTCCGGGTGGAACTGTACGC
    CGTAGAAGCGTTTTTCTTCGTTGGCCATAATGGCGAACGGGCAGCTCTCGGTGCTGGCGACGGTCACGAA
    GTCGGACGGAATCGCCGTCACTTTATCGCCGTGGCTCATCCACACGTCCAGCAGCGGTTTGCCGTCTGCG
    GTCAGGGAATCTTCAATACCGCGAACCAGCGCGCTGTCGGTCAACACTTCGACCTGCGCATAACCAAATT
    CACGCTCATTAGAACCTTCTACATGACCGCCAAGCTGCATCGCCATGGTCTGCATACCATAGCAAACGCC
    AAATACCGGCACGCCTGCTTCAAAGACATACTGCGGCGCGCGCGGGCTGTTTTCTTCGGTGGTGCTTTCC
    GGGCCGCCGGAAAGAATAATGCCGCTTGGGTTGAAGTCACGAATTTGTGCTTCTGTCACATCCCACGCCC
    ACAGTTCACAGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTC
    CAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCA
  • Protein Sequence : Show Sequence
    >gi|207857920|ref|YP_002244571.1| GMP synthase [Salmonella enterica subsp. enterica serovar Enteritidis str. P125109]
    MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEAQIRDFNPSGIILSGGPESTTEENSPR
    APQYVFEAGVPVFGVCYGMQTMAMQLGGHVEGSNEREFGYAQVEVLTDSALVRGIEDSLTADGKPLLDVW
    MSHGDKVTAIPSDFVTVASTESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTP
    AKIIDDAVARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVMDMFGDH
    FGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQGTIYPDVIESAASATG
    KAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELGLPYDMLYRHPFPGPGLGVRVLGEVKKEY
    CDLLRRADAIFIEELRKADLYDKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPY
    DFLGRVSNRIINEVNGISRVVYDISGKPPATIEWE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaBA and clpP mutant (CVD 1941) is attenuated in mice after oral immunization. This mutant is also protective in mice and had a vaccine efficacy of 76% against challenge with wild type Salmonella Enteritidis (Tennant et al., 2011).
  • Related Vaccine(s): Salmonella Enteritidis guaBA/clpP mutant vaccine
27. guaA from S. typhimurium str. LT2
  • Gene Name : guaA from S. typhimurium str. LT2
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1254032
  • NCBI Protein GI : 16765830
  • Locus Tag : STM2510
  • Genbank Accession : AE006468
  • Protein Accession : NP_461445
  • Taxonomy ID : 700720
  • Gene Starting Position : 2622804
  • Gene Ending Position : 2624381
  • Gene Strand (Orientation) : -
  • Protein Name : GMP synthase
  • Protein pI : 5.06
  • Protein Weight : 53458.79
  • Protein Length : 525
  • Protein Note : contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway
  • DNA Sequence : Show Sequence
    >gi|16763390:2622804-2624381 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    CTTATTCCCACTCAATGGTAGCCGGCGGTTTACCGCTGATGTCATACACCACACGGGAAATCCCGTTGAC
    TTCATTGATGATGCGGTTGGAAACGCGACCGAGGAAGTCATACGGCAGGTGCGCCCAGTGTGCGGTCATA
    AAGTCGATGGTTTCGACGGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATAACGCCAA
    CGGAACGAACCGGCAGGAAGACGGTAAACGCCTGACTGACTTTGTCGTACAGATCCGCTTTGCGCAGCTC
    TTCAATGAAGATAGCGTCAGCACGGCGCAGCAGGTCGCAGTACTCTTTCTTCACTTCACCCAGAACACGA
    ACGCCGAGGCCCGGCCCCGGGAACGGATGGCGATACAGCATGTCATACGGCAGGCCCAGCTCCAGACCAA
    TCTTACGCACTTCGTCTTTGAACAGCTCTTTCAGCGGTTCAACCAGCCCCATCTTCATCTCTTTCGGCAA
    GCCGCCGACATTGTGGTGAGATTTGATGACGTGCGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACG
    TCAGGGTAAATGGTGCCTTGCGCCAGCCACTTCACGTCTTCCAGTTTGAGCGCTTCTTCGTCGAACACTT
    CTACAAAAACACGACCGATGATCTTACGCTTGGCTTCCGGATCGTTCTCGCCAGCCAGCGCGGACAGGAA
    GCGCTCTTCTGCCGGTACGTGAACGATATTCAGGCCAAAATGGTCGCCAAACATGTCCATCACCTGCTCG
    GCTTCGTTGAGACGCAGCAGGCCGTTGTCGACGAATACACAGGTCAGATTTTTACCGATCGCGCGGTGCA
    GCAGCATTGCGGTGACGGAAGAATCCACGCCACCAGAGAGGCCGAGGATCACTTTGTCGTCGCCCACCTG
    CTCGCGAATGCGCGCCACGGCGTCGTCGATGATCTTCGCCGGCGTCCACAACGCTTCACACTGGCAGATA
    TCGCGTACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTGTGAGTCACTTCCGGGTGGAACTGTACAC
    CGTAGAAGCGTTTTTCTTCGTTGGCCATAATGGCGAACGGGCAGCTCTCGGTGCTGGCGACGGTCACGAA
    GTCGGACGGAATCGCCGTCACTTTATCGCCGTGGCTCATCCACACGTCCAGCAGCGGTTTGCCGTCTGCG
    GTCAGGGAATCTTCAATACCGCGAACCAGCGCGCTGTCGGTCAACACTTCGACCTGCGCATAACCAAATT
    CACGCTCATTAGAACCTTCTACATGACCGCCAAGCTGCATCGCCATGGTCTGCATACCATAGCAAACGCC
    AAATACCGGCACGCCTGCTTCAAAGACATACTGCAGCGCGCGCGGGCTGTTTTCTTCGGTGGTGCTTTCC
    GGGCCGCCGGAAAGAATAATGCCGCTTGGGTTGAAGTCACGAATTTGTGCTTCTGTCACATCCCACGCCC
    ACAGTTCACAGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTC
    CAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCA
  • Protein Sequence : Show Sequence
    >gi|16765830|ref|NP_461445.1| GMP synthase [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEAQIRDFNPSGIILSGGPESTTEENSPR
    ALQYVFEAGVPVFGVCYGMQTMAMQLGGHVEGSNEREFGYAQVEVLTDSALVRGIEDSLTADGKPLLDVW
    MSHGDKVTAIPSDFVTVASTESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTP
    AKIIDDAVARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVMDMFGDH
    FGLNIVHVPAEERFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQGTIYPDVIESAASATG
    KAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELGLPYDMLYRHPFPGPGLGVRVLGEVKKEY
    CDLLRRADAIFIEELRKADLYDKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPY
    DFLGRVSNRIINEVNGISRVVYDISGKPPATIEWE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaBA and clpP mutant (CVD 1921) of Salmonella Typhimurium is attenuated in mice after oral immunization. The mutant was also protective, rendering a vaccine efficacy of 86% in mice against challenge with wild type Salmonella Typhimurium (Tennant et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium guaBA/clpP mutant vaccine , Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine
28. guaB
  • Gene Name : guaB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1254033
  • NCBI Protein GI : 16765831
  • Locus Tag : STM2511
  • Genbank Accession : AE006468
  • Protein Accession : NP_461446
  • Taxonomy ID : 700720
  • Gene Starting Position : 2624451
  • Gene Ending Position : 2625917
  • Gene Strand (Orientation) : -
  • Protein Name : inosine 5'-monophosphate dehydrogenase
  • Protein pI : 6.59
  • Protein Weight : 47186.87
  • Protein Length : 488
  • Protein Note : catalyzes the synthesis of xanthosine monophosphate by the NAD+ dependent oxidation of inosine monophosphate
  • DNA Sequence : Show Sequence
    >gi|16763390:2624451-2625917 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    ATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTGGTGATGGTCACGTCGTGAACGTGGCTTTCCTGAAT
    GCCCGCACCGCTGATACGCACAAACTCCGCTTTAGTACGCAGTTCGTCGATGGTAGCACAACCGGTCAGC
    CCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGC
    GACCTTCGATACCTTCCGGCACCAGTTTGTCAGCGGCGTTGTCGCTCTGGAAGTAACGGTCGGAGGAACC
    TTTGGACATCGCACCCAGCGAGCCCATGCCGCGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATT
    TCGCCCGGGGATTCTTCCGTACCCGCCAGCATGGAACCCACCATTACCGCGCTTGCACCTGCGGCAATCG
    CTTTGGCTATGTCGCCGGAGAAACGGATACCGCCGTCAGCGATAACCGGAATACCGGTACCTTCCAGCGC
    TTCAACTGCGTCAGAAACAGCGGTGATCTGCGGAACGCCCACGCCAGTCACGATACGAGTGGTACAGATG
    GAACCCGGGCCAATACCGACTTTCACCGCGCTGCAACCGGCTTCCGCCAGTGCGCGAGCGCCTGCGCCTG
    TCGCGACGTTGCCGCCGATGATTTGCAGGTCAGGATATTTAGCACGGGTTTCACGGATACGTTGCAACAC
    GCCTTCTGAATGACCGTGAGAGGAGTCGATCAGCAGTACGTCAACGCCTGCCGCCACCAGCGCGTCAACG
    CGCTCTTCGTTGCCCGCGCCTGCGCCGACCGCCGCGCCGACACGTAAACGGCCCTGCTCATCTTTGCAGG
    AGTTTGGTTTACGTTCCGCTTTCTGGAAATCTTTTACGGTAATCATGCCAAGCAGATGGAAGTTATCATC
    AACGACCAGCGCTTTTTCTACGCGTTTTTCGTGCATTTTTGCCAGCACGACTTCACGGGCTTCGCCTTCA
    CGAACGGTCACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTGGTTCAGGTCAGTCACAAAAC
    GCACGTCACGACCGGTGATGATACCCACCAGCTCGTTATCTTCAGTCACCACCGGATAGCCCGCAAAACC
    GTTACGCTCGGTCAGGGCTTTCACTTCATGCAACGTGGTGGTTGGCAGGACGGTCTGCGGGTCGGTCACT
    ACGCCGGACTCGTGTTTCTTCACGCGGCGAACTTCTTCCGCCTGGCGCTCAATGGACATGTTTTTGTGGA
    TAAAACCAATGCCGCCTTCCTGGGCCAGGGCAATTGCCAGGCGCGCTTCCGTCACGGTGTCCATCGCCGC
    AGAAAGCATAGGAATATTCAGACGAATAGTTTTCGTCAACTGCGTGCTGAGATCGGCAGTATTCGGCAAA
    ACGGTGGAGTGAGCGGGAACAAGGAGGACGTCGTCAAACGTCAGGGCTTCTTTAGCGATACGTAGCA
  • Protein Sequence : Show Sequence
    >gi|16765831|ref|NP_461446.1| inosine 5'-monophosphate dehydrogenase [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPMLSAAMDTVTEARLAIALAQEGGIGFI
    HKNMSIERQAEEVRRVKKHESGVVTDPQTVLPTTTLHEVKALTERNGFAGYPVVTEDNELVGIITGRDVR
    FVTDLNQPVSVYMTPKERLVTVREGEAREVVLAKMHEKRVEKALVVDDNFHLLGMITVKDFQKAERKPNS
    CKDEQGRLRVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGNVAT
    GAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVSDAVEALEGTGIPVIADGGIRFSGDIAKA
    IAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLGAMSKGSSDRYFQSDNAADKLVPEGIEGR
    VAYKGRLKEIIHQQMGGLRSCMGLTGCATIDELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaBA and clpP mutant (CVD 1921) of Salmonella Typhimurium is attenuated in mice after oral immunization. The mutant was also protective, rendering a vaccine efficacy of 86% in mice against challenge with wild type Salmonella Typhimurium (Tennant et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium guaBA/clpP mutant vaccine , Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine
29. guaB from S. enteritidis str. P125109
  • Gene Name : guaB from S. enteritidis str. P125109
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Enteritidis str. P125109
  • NCBI Gene ID : 6949287
  • NCBI Protein GI : 207857921
  • Locus Tag : SEN2491
  • Genbank Accession : AM933172
  • Protein Accession : YP_002244572
  • Taxonomy ID : 550537
  • Gene Starting Position : 2625484
  • Gene Ending Position : 2626950
  • Gene Strand (Orientation) : -
  • Protein Name : inosine 5'-monophosphate dehydrogenase
  • Protein pI : 6.59
  • Protein Weight : 47186.87
  • Protein Length : 488
  • Protein Note : catalyzes the synthesis of xanthosine monophosphate by the NAD+ dependent oxidation of inosine monophosphate
  • DNA Sequence : Show Sequence
    >gi|207855516:2625484-2626950 Salmonella enterica subsp. enterica serovar Enteritidis str. P125109 chromosome, complete genome
    ATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTGGTGATGGTCACGTCGTGAACGTGGCTTTCCTGGAT
    ACCCGCACCGCTGATACGCACAAACTCCGCTTTAGTACGCAGTTCGTCGATGGTAGCACAACCGGTCAGC
    CCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGC
    GGCCTTCGATACCTTCCGGCACCAGTTTGTCGGCGGCGTTGTCGCTCTGGAAGTAACGGTCGGAGGAACC
    TTTGGACATCGCGCCCAGAGAACCCATACCGCGATAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATT
    TCGCCCGGGGATTCTTCGGTACCGGCCAGCATAGAACCCACCATTACCGCGCTCGCGCCTGCGGCGATGG
    CTTTGGCGATGTCGCCGGAGAAACGGATACCGCCGTCAGCGATAACCGGAATCCCGGTGCCTTCCAGCGC
    TTCCACCGCGTCAGAAACAGCGGTGATCTGCGGAACGCCCACACCAGTCACGATACGAGTCGTACAGATG
    GAACCCGGGCCGATACCCACTTTCACCGCGCTGCAACCGGCTTCCGCCAGTGCGCGAGCGCCTGCGCCCG
    TCGCAACGTTGCCGCCGATGATTTGCAGGTCAGGATATTTAGCACGCGTCTCACGGATACGTTGCAACAC
    GCCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGTACGTCAACGCCTGCCGCCACCAGCGCGTCAACG
    CGCTCTTCGTTGCCCGCGCCTGCGCCGACCGCCGCGCCGACACGTAAACGGCCCTGCTCATCTTTACAGG
    AGTTTGGTTTACGTTCCGCTTTCTGGAAATCTTTTACGGTAATCATGCCAAGCAGATGGAAGTTATCATC
    AACGACCAGCGCTTTTTCTACGCGTTTTTCGTGCATTTTTGCCAGCACGACTTCACGGGCTTCGCCTTCA
    CGAACGGTCACCAGACGCTCTTTCGGTGTCATGTAGACACTCACCGGCTGGTTCAGGTCAGTCACAAAAC
    GCACGTCACGACCGGTGATGATACCCACCAGCTCGTTATCTTCAGTCACCACCGGATAGCCCGCAAAACC
    GTTACGCTCGGTCAGGGCTTTCACTTCATGCAACGTGGTGGTTGGCAGGACGGTCTGCGGGTCGGTCACT
    ACGCCGGACTCGTGTTTCTTCACGCGGCGAACTTCTTCCGCCTGGCGCTCAATAGACATGTTTTTGTGGA
    TAAAACCAATGCCGCCTTCCTGGGCCAGGGCAATTGCCAGGCGCGCTTCCGTCACGGTGTCCATCGCCGC
    AGAAAGCATAGGAATATTCAGACGAATAGTTTTCGTCAACTGCGTGCTGAGATCGGCAGTATTCGGCAAA
    ACGGTGGAGTGAGCGGGAACAAGGAGGACGTCGTCAAACGTCAGAGCTTCTTTAGCGATACGTAGCA
  • Protein Sequence : Show Sequence
    >gi|207857921|ref|YP_002244572.1| inosine 5'-monophosphate dehydrogenase [Salmonella enterica subsp. enterica serovar Enteritidis str. P125109]
    MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPMLSAAMDTVTEARLAIALAQEGGIGFI
    HKNMSIERQAEEVRRVKKHESGVVTDPQTVLPTTTLHEVKALTERNGFAGYPVVTEDNELVGIITGRDVR
    FVTDLNQPVSVYMTPKERLVTVREGEAREVVLAKMHEKRVEKALVVDDNFHLLGMITVKDFQKAERKPNS
    CKDEQGRLRVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGNVAT
    GAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVSDAVEALEGTGIPVIADGGIRFSGDIAKA
    IAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLGAMSKGSSDRYFQSDNAADKLVPEGIEGR
    VAYKGRLKEIIHQQMGGLRSCMGLTGCATIDELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaB mutant, in combination with a fliC mutant, is attenuated in mice and chickens and induced significant protection from challenge with wild type S. enterica (Adriaensen et al., 2007).
  • Related Vaccine(s): Salmonella enterica fliC/guaB mutant vaccine , Salmonella Enteritidis guaBA/clpP mutant vaccine
30. gyrA
  • Gene Name : gyrA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1253794
  • NCBI Protein GI : 16765599
  • Locus Tag : STM2272
  • Genbank Accession : AE006468
  • Protein Accession : NP_461214
  • Taxonomy ID : 700720
  • Gene Starting Position : 2373710
  • Gene Ending Position : 2376346
  • Gene Strand (Orientation) : -
  • Protein Name : DNA gyrase subunit A
  • Protein pI : 4.73
  • Protein Weight : 89524.22
  • Protein Length : 878
  • Protein Note : negatively supercoils closed circular double-stranded DNA
  • DNA Sequence : Show Sequence
    >gi|16763390:2373710-2376346 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    ATTACTCGTCAGCGTCATCCGCAACGTCGTCATCGCTTTCCGCTTCCGGGGCGATATCCTCATCCCCTTC
    CGCCACGCTGCCGTCGATAGCGTCGAGTTCTTCGTCATCTACCGGTTCAGCAACGCGTTGCAGACCCACC
    ACGTTTTCATCTTCCGCCGTGCGGATAAGGATAACGCCCTGGGTATTACGTCCCACTACGCTGATCTCGG
    ACACACGGGTACGCACCAGAGTACCGGCATCCGTGATCATCATGATCTGGTCGCAATCGTCTACCTGTAC
    CGCACCGACAACGGAACCGTTGCGCTCGGTCACTTTGATAGAGATAACGCCCTGCGTCGCACGAGACTTG
    GTCGGGTACTCGTCCGCTGCGGTACGCTTCCCGTAGCCGTTTTGCGTTACGGTCAGAATAGCGCCTTCGC
    CGCGTGGGATGATCAGAGAGACGACTTTATCGTCTCCCGCCAGCTTAATACCGCGCACACCGGTCGCGGT
    ACGCCCCATCGCACGGACGGCGTCTTCTTTGAAGCGCACCACTTTACCCGCGGCCGAGAACAGCATGACT
    TCGTCAGAACCAGAAGTCAGGTCAACGCCAATCAGCTCGTCGCCGTCGTTGAGGTTCACCGCGATAATAC
    CGGCGGAACGCGGACGGCTGAATTCGGTCAGCGCCGTTTTCTTCACGGTACCGCTGGCGGTCGCCATAAA
    GACGTTGACGCCTTCTTCATACTCACGAACCGGCAGAATCGCGGTGATACGTTCGTTGGCTTCCAGCGGC
    AGCAGGTTGACGATCGGACGACCGCGCGCGCCGCGGCTGGCTTCCGGCAGCTGATAGACCTTCATCCAGT
    ACAGACGGCCCCGGCTGGAGAAGCAGAGGATGGTGTCATGGGTGTTAGCCACCAGCAGGCGGTCGATAAA
    GTCTTCTTCTTTAATACGCGCGGCAGATTTACCTTTCCCACCACGACGTTGCGCTTCGTAATCTGTCAGC
    GGTTGATATTTGACGTAACCCTGGTGAGACAGCGTCACGACAACATCTTCCTGGCTAATCAGATCTTCGA
    TATTAATATCGGCGCTGTTGGCGGTGATTTCGGTACGACGCTCATCGCCGAACTGATCGCGAATTAACTC
    CATCTCTTCGCGGATCACTTCCATCAGGCGATCGGCGCTGCCCAGAATGTGCAGCAATTCAGCAATCTGC
    TCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCATGCTCCAGGCCGGTCAGTTTCTGCAAACGCAGAT
    CCAGAATCGCCTGCGCCTGCTGTTCAGTCAGGTAGTACTGACCGTCACGCACGCCAAATTCTGGCTCCAG
    CCATTCCGGACGCGCGGCGTCATCACCAGCGCGCTCCAGCATCGCAGCAACGTTGCCCAGATCCCACGGA
    CGCGAAATCAGCGCCGCTTTTGCTTCCGCCGGCGTTGGCGCGCGGCGAATCAGTTCGATAATCGGGTCGA
    TGTTGGCCAGCGCAATCGCCAGAGCTTCAAGGATATGCGCACGGTCACGGGCTTTACGCAGTTCAAAAAT
    AGTCCGACGCGTCACCACTTCACGGCGGTGGCGCACGAACGCTGAAATGATATCTTTCAGGTTCATGATC
    TTCGGCTGGCCGTGATGCAGCGCCACCATGTTAATACCGAAGGAAACCTGTAGCTGGGTCTGGGAGTAGA
    GATTATTAAGCACCACCTCGCCCACCGCATCGCGTTTCACTTCAATCACGATGCGCATCCCGTCTTTGTC
    GGATTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTATCTTTCACCAGCTCGGCGATTTTCTCGATC
    AGGCGCGCTTTGTTCACCTGATAGGGAATTTCATGGACGATGATGGTTTCACGGCCCGTTTTGGCGTCAG
    CTTCAACTTCCGCGCGGGCGCGAATGTACACTTTGCCACGACCGGTGCGGTAGGCTTCTTCGATACCACG
    ACGACCGTTGATGATCGCGGCGGTCGGGAAGTCCGGCCCCGGAATATGTTCCATCAGCCCTTCAATGCTG
    ATGTCTTCGTTGTCGATATACGCCAGGCAGCCGTTAATCACTTCCGTCAGGTTGTGCGGCGGGATATTCG
    TCGCCATACCTACTGCGATACCGGAAGAACCGTTCACCAGCAGATTCGGAATTTTGGTCGGCATGACGTC
    CGGAATTTTTTCCGTACCGTCATAGTTATCCACGAAATCCACCGTCTCTTTTTCGAGATCGGCCATCAGT
    TCGTGGGCGATTTTCGCCAGACGGATCTCCGTATAACGCATTGCCGCCGCGGAGTCGCCGTCAATAGAAC
    CGAAGTTACCCTGACCATCCACCAGCATGTAACGCAGCGAGAATGGCTGCGCCATACGAACGATGGTGTC
    ATACACTGCGGAATCGCCGTGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTA
    TAGGCTTTGTTCCAGTCATTGCCCAATACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGC
    CATCTCGGACATCCGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAG
    CTCCTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCA
  • Protein Sequence : Show Sequence
    >gi|16765599|ref|NP_461214.1| DNA gyrase subunit A [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPVHRRVLYAMNVLGNDWNKAYKKSARVV
    GDVIGKYHPHGDSAVYDTIVRMAQPFSLRYMLVDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEK
    ETVDFVDNYDGTEKIPDVMPTKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDNEDISIEGLME
    HIPGPDFPTAAIINGRRGIEEAYRTGRGKVYIRARAEVEADAKTGRETIIVHEIPYQVNKARLIEKIAEL
    VKDKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINMVALHHGQPKIMNLKDI
    ISAFVRHRREVVTRRTIFELRKARDRAHILEALAIALANIDPIIELIRRAPTPAEAKAALISRPWDLGNV
    AAMLERAGDDAARPEWLEPEFGVRDGQYYLTEQQAQAILDLRLQKLTGLEHEKLLDEYKELLEQIAELLH
    ILGSADRLMEVIREEMELIRDQFGDERRTEITANSADINIEDLISQEDVVVTLSHQGYVKYQPLTDYEAQ
    RRGGKGKSAARIKEEDFIDRLLVANTHDTILCFSSRGRLYWMKVYQLPEASRGARGRPIVNLLPLEANER
    ITAILPVREYEEGVNVFMATASGTVKKTALTEFSRPRSAGIIAVNLNDGDELIGVDLTSGSDEVMLFSAA
    GKVVRFKEDAVRAMGRTATGVRGIKLAGDDKVVSLIIPRGEGAILTVTQNGYGKRTAADEYPTKSRATQG
    VISIKVTERNGSVVGAVQVDDCDQIMMITDAGTLVRTRVSEISVVGRNTQGVILIRTAEDENVVGLQRVA
    EPVDDEELDAIDGSVAEGDEDIAPEAESDDDVADDADE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A gyrA-cpxA-rpoB Salmonella Typhimurium mutant was attenuated and protected against challenge in pigs (Roesler et al., 2004).
  • Related Vaccine(s): Salmonella Typhimurium gyrA/cpxA/rpoB mutant vaccine
31. hfq
  • Gene Name : hfq
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1255887
  • NCBI Protein GI : 16767607
  • Locus Tag : STM4361
  • Genbank Accession : AE006468
  • Protein Accession : NP_463222
  • 3D structure: PDB ID : 2YLB
  • Taxonomy ID : 700720
  • Gene Starting Position : 4604574
  • Gene Ending Position : 4604882
  • Gene Strand (Orientation) : +
  • Protein Name : RNA-binding protein Hfq
  • Protein pI : 7.9
  • Protein Weight : 10240.49
  • Protein Length : 102
  • Protein Note : Stimulates the elongation of poly(A) tails
  • DNA Sequence : Show Sequence
    >gi|16763390:4604574-4604882 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    AATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCATTGCGTCGGGAACGTGTTCCAGTTTCT
    ATTTATTTGGTGAATGGTATTAAGCTGCAAGGTCAAATCGAGTCCTTTGATCAGTTCGTGATCCTGTTGA
    AGAACACGGTCAGCCAGATGGTTTATAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCCCA
    TCACAGCAACAATGCCGGTGGCGGCGCCAGCAATAACTACCATCACGGTAGCAACGCGCAGGGGTCTACT
    GCGCAACAGGACAGCGAAGAGACTGAATA
  • Protein Sequence : Show Sequence
    >gi|16767607|ref|NP_463222.1| RNA-binding protein Hfq [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVILLKNTVSQMVYKHAISTVVPSRPVSH
    HSNNAGGGASNNYHHGSNAQGSTAQQDSEETE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Oral immunization with the Salmonella hfq deletion mutant efficiently protects mice against subsequent oral challenge with virulent strain of Salmonella Typhimurium (Allam et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium hfq mutant vaccine
32. HslU
  • Gene Name : HslU
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • VO ID : VO_0011266
  • NCBI Protein GI : 9650897
  • Other Database IDs : CDD:167927
    CDD:99707
    CDD:158411
    GOA:O30911
    InterPro: IPR003593
    InterPro: IPR004491
    InterPro: IPR013093
    UniProtKB/UniProt: O30911
  • Taxonomy ID : 90371
  • Protein Name : heat shock protein
  • Protein Length : 443
  • Protein Note : ATP-dependent protease ATP-binding subunit HslU; Provisional; PRK05201
  • Protein Sequence : Show Sequence
    >gi|9650897|emb|CAC00720.1| heat shock protein [Salmonella typhimurium]
    MSEMTPREIVSELNKHIIGQDNAKRSVAIALRNRWRRMQLDEELRHEVTPKNILMIGPTGVGKTEIARRL
    AKLANAPFIKVEATKFTEVGYVGKEVDSIIRDLTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAK
    NNWGQAEQQQEPSAARQTFRKKLREGQLDDKEIEINLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQK
    QKPRKLKIKDAMKLLVEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGETSGPDVSREGVQRD
    LLPLVEGCTVSTKHGMVKTDHILFIASGAFQVAKPSDLIPELQGRLPIRVELQALTTSDFERILTEPNAS
    VTVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNETTENIGARRLHTVLERLMEEISYNASDLHGQNITI
    DAEYVSKHLDALVADEDLSRFIL
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Study demonstrated that a recombinant construction of heat-shock protein (hslU) with lypopolysaccharide (rcHSP-LPS) administered 24 hours before inoculation induced resistance to S. typhimurium infection. Protection formed after 3 injections of rcHSP-LPS with 10 mcg in each or single injection with 100 mcg/mouse. Forty to eighty percent of immunized mice survived after challenge while 90% of control animals died. Destruction of the HSP by boiling of the construction led to loss of protective effect (Vorob'ev et al., 2009).
33. HtrA
  • Gene Name : HtrA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. CT18
  • NCBI Gene ID : 1246721
  • NCBI Protein GI : 16759200
  • Locus Tag : STY0231
  • Genbank Accession : NC_003198.1
  • Protein Accession : NP_454817.1
  • Taxonomy ID : 28901
  • Gene Starting Position : 241500
  • Gene Ending Position : 242927
  • Gene Strand (Orientation) : +
  • Protein Name : protease DO precursor; heat shock protein HtrA
  • Protein pI : 9.27
  • Protein Weight : 49287.9
  • Protein Length : 475
  • Protein Note : Fasta hit to DEGQ_ECOLI (455 aa), 57% identity in 478 aa overlap Orthologue of E. coli htrA (HTRA_ECOLI); Fasta hit to HTRA_ECOLI (474 aa), 92% identity in 475 aa overlap
  • DNA Sequence : Show Sequence
    >GeneID|1246721 [Salmonella enterica subsp. enterica serovar Typhi str. CT18 ] 241500..242927
    atgaaaaaaaccacattagcaatgagtgcactggctctgagtttaggtttggcattgtcgcctctgtctg
    ccacggcggctgaaacgtcctcttcagcaatgactgcccagcagatgccaagcctggcaccgatgctcga
    aaaagtgatgccatcggtggtcagtattaatgtagaaggtagcaccacggtgaatacgccgcgtatgccg
    cgtaatttccagcaattctttggcgatgactccccgttctgccaggacggttctccgttccagaattctc
    cgttctgccagggcggcggtaacggcggcaacggcggtcaacaacagaaattcatggcgctgggctccgg
    cgtaattattgacgccgcgaagggctacgtcgtcaccaacaaccacgtggttgataacgccagcgtgatt
    aaagtacagcttagcgatgggcgtaaattcgatgctaaagtggtgggcaaagatccgcgttctgatatcg
    cgctgattcaaattcagaatccgaagaacctgacggcgattaagctggcggactccgacgcgctgcgcgt
    gggggattataccgtcgctattggtaacccgtttggtctgggcgaaacggtgacgtcaggtatcgtttcg
    gcgctggggcgtagcggcctgaacgtagaaaattacgagaactttattcagaccgacgccgcgattaacc
    gcggtaactccggcggcgcgctggtgaacctgaacggtgagctgatcggtattaacaccgcgattctggc
    gccggacggcggcaacatcggtatcggcttcgctatccccagtaacatggtgaaaaacctgacgtcgcag
    atggtggaatacggccaggtgaaacgcggcgaactggggatcatggggactgagctgagctctgagctgg
    cgaaagcgatgaaagtcgacgcccagcgtggcgcgttcgtcagccaggtgatgccgaattcgtccgcagc
    gaaagcgggtatcaaagccggggatgtcattacctcgctgaacggtaaaccgatcagcagctttgcggcg
    ctgcgcgctcaggtcggcactatgccggtcggcagcaaaatcagcctcggtctgctgcgtgaaggtaaag
    cgattacggttaatctggaactgcagcagagcagccagagtcaggttgattccagcaccatcttcagcgg
    gattgagggcgctgaaatgagtaataaaggccaggataaaggcgttgtggtgagcagcgtgaaagcgaac
    tcacccgccgcgcaaattggcctcaaaaaaggcgatgtgattatcggcgctaaccagcagccggtgaaaa
    atatcgccgagctgcgtaagattctcgacagcaagccgtcggtgctggcgctgaatattcagcgtggtga
    tagttctatttatttgctgatgcagtaa
  • Protein Sequence : Show Sequence
    >gi|16759200|ref|NP_454817.1| protease DO precursor; heat shock protein HtrA [Salmonella enterica subsp. enterica serovar Typhi str. CT18 ]
    MKKTTLAMSALALSLGLALSPLSATAAETSSSAMTAQQMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMP
    RNFQQFFGDDSPFCQDGSPFQNSPFCQGGGNGGNGGQQQKFMALGSGVIIDAAKGYVVTNNHVVDNASVI
    KVQLSDGRKFDAKVVGKDPRSDIALIQIQNPKNLTAIKLADSDALRVGDYTVAIGNPFGLGETVTSGIVS
    ALGRSGLNVENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNLTSQ
    MVEYGQVKRGELGIMGTELSSELAKAMKVDAQRGAFVSQVMPNSSAAKAGIKAGDVITSLNGKPISSFAA
    LRAQVGTMPVGSKISLGLLREGKAITVNLELQQSSQSQVDSSTIFSGIEGAEMSNKGQDKGVVVSSVKAN
    SPAAQIGLKKGDVIIGANQQPVKNIAELRKILDSKPSVLALNIQRGDSSIYLLMQ
  • Related Vaccine(s): CVD 909
34. htrA
  • Gene Name : htrA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium SL1344
  • NCBI Protein GI : 301156836
  • Locus Tag : STM0209
  • Genbank Accession : AE006468
  • Protein Accession : NP_459214
  • Other Database IDs : CDD:182855
    CDD:199257
    CDD:29044
    UniProtKB/TrEMBL: E1W881
  • Taxonomy ID : 216597
  • Gene Starting Position : 244491
  • Gene Ending Position : 245918
  • Gene Strand (Orientation) : +
  • Protein Name : protease DO precursor; heat shock protein HtrA
  • Protein pI : 9.27
  • Protein Weight : 45544.93
  • Protein Length : 475
  • Protein Note : serine endoprotease; Provisional
  • DNA Sequence : Show Sequence
    >gi|16763390:244491-245918 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    CATGAAAAAAACCACATTAGCAATGAGTGCACTGGCTCTGAGTTTAGGTTTGGCATTGTCGCCTCTGTCT
    GCCACGGCGGCTGAAACGTCCTCTTCAGCAATGACTGCCCAGCAGATGCCAAGCCTGGCACCGATGCTCG
    AAAAAGTGATGCCATCGGTGGTCAGTATTAATGTTGAAGGTAGCACCACGGTGAATACGCCGCGTATGCC
    GCGTAATTTCCAGCAGTTCTTTGGCGATGACTCCCCGTTCTGCCAGGACGGTTCTCCGTTCCAGAATTCT
    CCGTTCTGCCAGGGCGGCGGTAACGGCGGCAACGGCGGTCAACAACAGAAATTCATGGCGCTGGGCTCCG
    GCGTAATTATTGACGCCGCGAAGGGCTACGTCGTCACCAACAACCACGTGGTTGATAACGCCAGCGTGAT
    TAAAGTACAGCTTAGCGATGGGCGTAAATTCGATGCTAAAGTGGTGGGCAAAGATCCGCGTTCTGATATC
    GCGCTGATTCAAATTCAGAATCCGAAGAACCTGACGGCGATTAAGCTGGCGGACTCCGACGCGCTGCGCG
    TGGGGGATTATACCGTCGCTATTGGTAACCCGTTTGGTCTGGGCGAAACGGTGACGTCAGGTATCGTTTC
    GGCGCTGGGGCGTAGCGGCCTGAACGTAGAAAATTACGAGAACTTTATTCAGACCGACGCCGCGATTAAC
    CGTGGTAACTCCGGCGGCGCGCTGGTGAACCTGAACGGTGAGCTGATCGGTATTAACACCGCGATTCTGG
    CGCCGGACGGCGGCAACATCGGTATCGGCTTCGCTATCCCCAGTAACATGGTGAAAAACCTGACGTCGCA
    GATGGTGGAATACGGCCAGGTGAAACGCGGCGAACTGGGGATCATGGGGACTGAGCTGAATTCCGAATTG
    GCGAAAGCGATGAAAGTCGACGCCCAGCGAGGCGCGTTCGTCAGCCAGGTGATGCCGAATTCGTCCGCGG
    CGAAAGCGGGTATCAAAGCCGGGGATGTCATTACCTCGCTGAACGGTAAACCGATCAGCAGCTTTGCGGC
    GCTGCGCGCTCAGGTCGGCACTATGCCGGTCGGCAGCAAAATCAGCCTCGGTCTGCTGCGTGAAGGTAAA
    GCGATTACGGTGAATCTGGAACTGCAGCAGAGCAGCCAGAGTCAGGTTGATTCCAGCACCATCTTCAGCG
    GGATTGAAGGCGCTGAAATGAGCAATAAAGGCCAGGATAAAGGCGTTGTGGTGAGCAGCGTGAAAGCGAA
    CTCACCCGCCGCGCAAATTGGCCTCAAAAAAGGCGATGTGATTATCGGCGCTAACCAGCAGCCGGTGAAA
    AATATCGCCGAGCTGCGTAAGATTCTCGACAGCAAGCCGTCGGTTCTGGCGCTGAATATTCAGCGTGGTG
    ATAGTTCTATTTATTTGCTGATGCAGTA
  • Protein Sequence : Show Sequence
    >gi|301156836|emb|CBW16312.1| protease DO precursor; heat shock protein HtrA [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
    MKKTTLAMSALALSLGLALSPLSATAAETSSSAMTAQQMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMP
    RNFQQFFGDDSPFCQDGSPFQNSPFCQGGGNGGNGGQQQKFMALGSGVIIDAAKGYVVTNNHVVDNASVI
    KVQLSDGRKFDAKVVGKDPRSDIALIQIQNPKNLTAIKLADSDALRVGDYTVAIGNPFGLGETVTSGIVS
    ALGRSGLNVENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNLTSQ
    MVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVMPNSSAAKAGIKAGDVITSLNGKPISSFAA
    LRAQVGTMPVGSKISLGLLREGKAITVNLELQQSSQSQVDSSTIFSGIEGAEMSNKGQDKGVVVSSVKAN
    SPAAQIGLKKGDVIIGANQQPVKNIAELRKILDSKPSVLALNIQRGDSSIYLLMQ
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A htrA mutant in Salmonella typhimurium is attenuated and provides protection in mice against an oral challenge with virulent S. typhimurium SL1344 (Chatfield et al., 1992).
  • Related Vaccine(s): Salmonella Typhimurium htrA mutant vaccine
35. IroN
  • Gene Name : IroN
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • VO ID : VO_0010997
  • NCBI Protein GI : 259475459
  • Other Database IDs : CDD:172107
    CDD:73259
  • Taxonomy ID : 90371
  • Gene Strand (Orientation) : ?
  • Protein Length : 751
  • Protein Note : outer membrane receptor FepA; Provisional; PRK13524
  • Protein Sequence : Show Sequence
    >gi|259475459|emb|CBF90638.1| unnamed protein product [Salmonella enterica subsp. enterica serovar Typhimurium]
    MNKKIHSLTLLVNLGIYGATLPVMAEDKTDSAALTNEDTIVVTAAQQNLQAPGVSTITADEIRKNPPARD
    VSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPENTLILIDGKPVTSRNSVRLGWRGERDTRGDTAWV
    PPEMIERIEVLRGPAAARYGNGAAGGVVNIITKKGGSEWHGSWNTYFNAPEHKDEGATKRTNFSLNGPLG
    GDFSFRLYGNLDKTQADARNINQGHQSERTGSYADTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
    RQGNLYAGDTQNTNTNQLVKDNYGKETNRLYRQNYSLTWNGGWNNGVTTSNWVQYEHTRNSRMPEGLAGG
    TEGIFDPKASQKYADADLNDVTLHSEVSLPFDLLVNQNLTLGTEWAQQRMKDQLSNSQTFMGGNIPGYSS
    TNRSPYSKAEIFSLFAENNMELTDSTMLTPGLRFDHHSIVGDNWSPSLNLSQGLGDDFTLKMGIARAYKA
    PSLYQTNPNYILYSKGQGCYATGAGTGIGCYMMGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRN
    KIEAGTVPLQRINNGKTDVYQWENVPKAVVEGLEGTLNVPVSDTVNWTNNVTYMLQSKNKETGERLSIIP
    QYTLNSTLSWQVRQDVSLQSTFTWYGKQEPKKYDYQGNPVTGTDKQAVSPYSIVGLSATWDVTKNVSLTG
    GVDNLFDKRLWREGNAQTVRDTQTGAYMAGAGAYTYNEPGRTWYMSINTHF
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Thirty-day-old chickens were immunized intramuscularly with purified IroN from Salmonella Typhimurium mixed with Freund's incomplete adjuvant; the chickens were then challenged intravenously with Salmonella Enteritidis. The mortality rate of immunized chickens was 10%. On the other hand, that of control chickens was 80% (Kaneshige et al., 2009).
  • Related Vaccine(s): Salmonella IroN Protein Vaccine
36. lon
  • Gene Name : lon
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6923289
  • NCBI Protein GI : 205351761
  • Locus Tag : SG0461
  • Genbank Accession : AM933173
  • Protein Accession : YP_002225562
  • Taxonomy ID : 550538
  • Gene Starting Position : 503376
  • Gene Ending Position : 505730
  • Gene Strand (Orientation) : ?
  • Protein Name : DNA-binding ATP-dependent protease La
  • Protein pI : 6.32
  • Protein Weight : 81259.62
  • Protein Length : 784
  • DNA Sequence : Show Sequence
    >gi|205351346:503376-505730 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
    TATGAATCCTGAGCGTTCTGAACGCATTGAAATCCCCGTATTGCCCTTGCGCGATGTGGTGGTTTATCCG
    CACATGGTCATACCCTTATTTGTAGGGCGGGAGAAATCTATCCGTTGTCTGGAAGCGGCTATGGACCATG
    ATAAAAAAATCATGCTGGTCGCCCAGAAGGAAGCATCGACGGATGAGCCGGGTGTAAACGATCTTTTCAC
    CGTCGGGACCGTGGCCTCTATTTTGCAGATGCTGAAACTGCCTGACGGCACCGTAAAAGTGCTGGTCGAG
    GGGTTACAGCGCGCGCGTATTTCCGCGCTGTCTGACAACGGCGAACATTTCTCCGCGAAGGCGGAGTACC
    TTGATTCGCCAGCCATTGATGAGCGTGAACAAGAAGTGCTGGTGCGTACCGCTATCAGCCAGTTCGAAGG
    CTACATCAAGCTGAACAAAAAAATTCCGCCGGAAGTGCTGACGTCACTCAATAGCATTGACGACCCTGCA
    CGTCTGGCTGACACCATCGCCGCGCATATGCCGCTGAAACTGGCGGATAAACAGTCCGTACTGGAGATGT
    CCGACGTTAACGAACGTCTGGAATATCTGATGGCGATGATGGAATCTGAAATCGATCTGCTGCAGGTTGA
    GAAACGCATTCGCAACCGCGTGAAAAAGCAGATGGAGAAATCCCAGCGCGAGTACTATCTGAACGAGCAA
    ATGAAAGCGATTCAGAAAGAGCTTGGAGAGATGGACGACGCGCCGGACGAAAACGAAGCGCTGAAGCGTA
    AGATCGACGCGGCGAAAATGCCGAAAGAGGCGAAAGAGAAAGCGGAAGCGGAACTGCAGAAGCTGAAAAT
    GATGTCTCCGATGTCGGCGGAAGCGACCGTGGTGCGCGGCTACATCGACTGGATGGTGCAGGTGCCGTGG
    AATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGCGCAGGAAATCCTCGATACTGACCATTATGGTC
    TGGAGCGCGTGAAAGACCGCATCCTTGAGTATCTCGCGGTTCAAAGCCGTGTAAATAAAATCAAGGGGCC
    GATCCTGTGTCTGGTGGGGCCGCCGGGGGTAGGTAAAACCTCGTTGGGCCAGTCCATCGCTAAAGCAACA
    GGGCGTAAATACATCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGCGGTCATCGCCGGA
    CTTACATTGGCTCTATGCCGGGTAAACTGATCCAAAAAATGGCCAAAGTGGGGGTTAAAAACCCACTGTT
    CCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGCGGCGATCCGGCTTCCGCACTGCTGGAAGTG
    CTGGATCCGGAGCAGAACGTGGCGTTCAGCGATCATTACCTGGAAGTGGATTACGATCTCAGCGATGTGA
    TGTTCGTCGCCACTTCCAACTCCATGAACATTCCGGCGCCGCTGCTGGATCGTATGGAAGTGATCCGTCT
    GTCCGGTTATACCGAAGACGAGAAGCTGAATATTGCTAAACGTCACCTGCTGCCGAAGCAGATCGAGCGT
    AACGCGCTGAAGAAAGGCGAACTGACGGTCGACGATAGCGCGATTATCGGTATTATCCGCTATTACACGC
    GTGAAGCGGGCGTTCGTAGTCTGGAGCGTGAAATCTCCAAACTGTGCCGCAAAGCGGTTAAACAACTGTT
    GCTCGATAAGTCGTTAAAACATATCGAAATTAACGGTGATAATCTGCACGACTACCTCGGTGTGCAGCGC
    TTTGACTATGGCCGCGCGGATAGCGAAAACCGCGTAGGTCAGGTGACGGGCCTGGCGTGGACGGAAGTGG
    GCGGCGATCTGTTGACCATCGAAACCGCCTGCGTCCCGGGCAAAGGCAAGTTGACCTACACCGGCTCGCT
    CGGTGAAGTGATGCAGGAATCCATTCAGGCGGCGCTGACCGTGGTGCGTGCGCGCGCGGAAAAACTGGGT
    ATTAATCCGGACTTTTACGAAAAACGCGATATCCACGTTCACGTGCCGGAAGGCGCAACGCCGAAAGACG
    GTCCAAGCGCCGGTATCGCGATGTGTACCGCGCTGGTTTCCTGCCTGACCGGTAATCCGGTGCGCGCAGA
    TGTGGCAATGACCGGTGAGATCACGCTGCGTGGTCAGGTGTTGCCGATCGGCGGCCTGAAAGAAAAACTG
    TTGGCGGCGCATCGCGGCGGGATTAAAACGGTCTTAATTCCGTTCGAAAATAAACGTGACCTGGAGGAAA
    TTCCGGACAACGTGATTGCCGATCTGGATATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACGCTTGC
    TCTGCAAAACGAACCGTCTGGAATGCAGGTTGTAACCGCAAAATA
  • Protein Sequence : Show Sequence
    >gi|205351761|ref|YP_002225562.1| DNA-binding ATP-dependent protease La [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
    MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAMDHDKKIMLVAQKEASTDEPGVNDLFT
    VGTVASILQMLKLPDGTVKVLVEGLQRARISALSDNGEHFSAKAEYLDSPAIDEREQEVLVRTAISQFEG
    YIKLNKKIPPEVLTSLNSIDDPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVE
    KRIRNRVKKQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQKLKM
    MSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRILEYLAVQSRVNKIKGP
    ILCLVGPPGVGKTSLGQSIAKATGRKYIRMALGGVRDEAEIRGHRRTYIGSMPGKLIQKMAKVGVKNPLF
    LLDEIDKMSSDMRGDPASALLEVLDPEQNVAFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRL
    SGYTEDEKLNIAKRHLLPKQIERNALKKGELTVDDSAIIGIIRYYTREAGVRSLEREISKLCRKAVKQLL
    LDKSLKHIEINGDNLHDYLGVQRFDYGRADSENRVGQVTGLAWTEVGGDLLTIETACVPGKGKLTYTGSL
    GEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGIAMCTALVSCLTGNPVRAD
    VAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFENKRDLEEIPDNVIADLDIHPVKRIEEVLTLA
    LQNEPSGMQVVTAK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A lon mutant, in combination with a cpxR mutation, is highly attenuated in mice and induces significant protection from challenge with wild type S. gallinarum (Matsuda et al., 2010).
  • Related Vaccine(s): Salmonella Gallinarum cpxR/Ion mutant vaccine
37. lppA
  • Gene Name : lppA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • NCBI Protein GI : 323129647
  • Other Database IDs : CDD:187093
  • Taxonomy ID : 909946
  • Gene Strand (Orientation) : ?
  • Protein Name : major outer membrane lipoprotein
  • Protein Length : 78
  • Protein Note : Murein lipoprotein [Cell envelope biogenesis, outer membrane]; cl11539
  • Protein Sequence : Show Sequence
    >gi|323129647|gb|ADX17077.1| major outer membrane lipoprotein [Salmonella enterica subsp. enterica serovar Typhimurium str. 4/74]
    MNRTKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQLSNDVNAMRSDVQAAKDDAARANQRLD
    NQATKYRK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An lppA mutant, in combination with an lppB/msbB mutation, is attenuated in mice and induces significant protection from challenge with wild type S. Typhimurium (Liu et al., 2008).
  • Related Vaccine(s): Salmonella Typhimurium IppA/IppB/msbB
38. lppB
  • Gene Name : lppB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
  • NCBI Protein GI : 267993263
  • Other Database IDs : CDD:187093
  • Taxonomy ID : 588858
  • Gene Strand (Orientation) : ?
  • Protein Name : putative methyl-accepting chemotaxis protein
  • Protein Length : 79
  • Protein Note : Murein lipoprotein [Cell envelope biogenesis, outer membrane]; cl11539
  • Protein Sequence : Show Sequence
    >gi|267993263|gb|ACY88148.1| putative methyl-accepting chemotaxis protein [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]
    MNRTNQLILGAVVLGSTLLAGCSSNAKIDQLSSDVQTLSAKVEQLSNDVNAMRSDVQAAKDDAARANQRL
    DNKVFRICK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An lppB mutant, in combination with an msbB mutant or an msbB/lppA mutant, is attenuated in mice and induces significant protection from challenge with wild type S. Typhimurium (Liu et al., 2008).
  • Related Vaccine(s): Salmonella Typhimurium IppA/IppB/msbB , Salmonella Typhimurium IppB/msbB mutant vaccine
39. msbB
  • Gene Name : msbB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium 14028
  • NCBI Protein GI : 4104731
  • Other Database IDs : ATCC:14028
    CDD:153246
  • Taxonomy ID : 90371
  • Gene Strand (Orientation) : ?
  • Protein Name : lipid A acyltransferase
  • Protein Length : 323
  • Protein Note : Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: LABLAT-like; cd07984
  • Protein Sequence : Show Sequence
    >gi|4104731|gb|AAD03801.1| lipid A acyltransferase [Salmonella enterica subsp. enterica serovar Typhimurium]
    METKKNNSEYIPEFEKSFRYPQYWGAWLGAAAMAGIALTPASFRDPLLATLGRFAGRLGKSSRRRALINL
    SLCFPQRSEAEREAIVDEMFATAPQAMAMMAELAMRGPKKIQQRVDWEGLEIIEEMRRNDEKVIFLVPHG
    WGVDIPAMLMASQGQKMAAMFHNQGNPVFDYIWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQ
    DHGPEHSEFVDFFATYKATLPAIGRLMKVCRARVIPLFPVYNGKTHRLTIQIRPPMDDLLTADDHTIARR
    MNEEVEIFVGPHPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An msbB mutant, in combination with an lppB or lppAB mutation, is attenuated in mice and induces significant protection from challenge with S. Typhimurium (Liu et al., 2008).
  • Related Vaccine(s): Salmonella Typhimurium IppA/IppB/msbB , Salmonella Typhimurium IppB/msbB mutant vaccine
40. NmpC
  • Gene Name : NmpC
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • NCBI Protein GI : 517159
  • Other Database IDs : CDD:174088
  • Taxonomy ID : 90371
  • Gene Strand (Orientation) : ?
  • Protein Name : NmpC protein
  • Protein Length : 282
  • Protein Note : synonym: Salmonella typhimurium
  • Protein Sequence : Show Sequence
    >gi|517159|dbj|BAA05056.1| NmpC protein [Salmonella enterica subsp. enterica serovar Typhimurium]
    EFKGNRTESQGADKDKTRLAFAGLKFADYGSFDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRT
    TGVATYRKTDFFGLVEGLNFAAQYQGKNDRDGAYESNGDGFGLSATYEYEGFGVGAAYAKSDRTNNQVKA
    ASNLNAAGKNAEVWAAGLKYDANNIYLATTYSETLNMTFGEDAAGDAFIANKTQNFEAVAQYQFDFGLRP
    SIAYLKSKGKNLGTYGDQDLVEYIDVGATYYFNKNMSTFVDYKINLLDDSDFTKAAKVSTDNIVAVGLNY
    QF
  • Molecule Role : Virmugen
  • Molecule Role Annotation : In a pig infection experiment including two oral immunizations with SalmoporcDeltaompD (OmpD, nmpC) and challenge with a multiresistant S. enterica serovar Typhimurium DT104 clinical isolate, we confirmed the protective efficacy of SalmoporcDeltaompD in pigs, showing a significant reduction of both clinical symptoms and colonization of lymph nodes and intestinal tract (Selke et al., 2007).
  • Related Vaccine(s): Salmonella Typhimurium ompD/nmpC mutant vaccine
41. nuoG
  • Gene Name : nuoG
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6924283
  • NCBI Protein GI : 205353438
  • Locus Tag : SG2352
  • Genbank Accession : AM933173
  • Protein Accession : YP_002227239
  • Taxonomy ID : 550538
  • Gene Starting Position : 2416815
  • Gene Ending Position : 2419541
  • Gene Strand (Orientation) : -
  • Protein Name : NADH dehydrogenase subunit G
  • Protein pI : 6.17
  • Protein Weight : 93802.85
  • Protein Length : 908
  • Protein Note : Catalyzes the transfer of electrons from NADH to quinone
  • DNA Sequence : Show Sequence
    >gi|205351346:2416815-2419541 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
    CTCATTGTTGCGCCTCCCGCAGATCCTCAAGACGCGCGCCAGCCAGAACCGGCGCGATGCCAGGCATACC
    CATCGGCAGCCCTACCTGCCCTGCCGCTAACCCTTCAGAGATTTCAACCGGCAGCGTCACCGTATTGCCA
    TCGTAGCTAAAGGAGACGCGCGTCCCGGCATTGACGCCCAACTTCGCGGCATCCGCCGGGTTAAGTTTGA
    TATACGGCTGCGGCATACGGCTCTGGAAGACCGGAGAACGCTGAGACAATCCGTCGCTGCCAAACAGGTG
    GTAATACGGCGCAATACGCCACTGACCGTCCTGCGCCTGGAAGCTTGCCGGCACGGTAGTGAAATAATCC
    AGACCGCCCTCAGTCGCTTCAATCAAACGCACGCCCGGATCGCCGTGACGCAGTTTGCCGCCCACTTCAT
    CCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCGAACGGAATTTCAGATCGCGG
    GGCAGTCGGTTGGTTATTCCCTTCCATCGAGAAGGCGAACATGGTGTCTTTATCCTGCGGCTGACGTGGT
    TCATGCACGCTGATATTGGCGCGCATCGCCGTACGACCGCTGTAACGATGCGGTTCGCGCGCCAGCTTCT
    GCCCACGAATGCGGAAGGTCGCATCCGGCGCGGCGTCTTTAATGCCGGCAAATTGCGGCATGGCGGCAAT
    GACCGCGTCGATCACATGGTCAAGCTGTGTCCAGTCCACTTCGCGGTTTTCGACGGTGCTGTGCAGCGAA
    TGCAGCCAGCGCCAGCTTTCCAGCATAATCGTCTTGTTATCGTAGTAGGCCGGATCATAAACCTGGAAGA
    AGCGCTGCGCGCGGCCTTCGTTGTTGATGACCGTACCATCGCTTTCGGCGAAGCTTGCCGCGGAAAGCAC
    CAGATGCGCGTTTTCCATAATCGCCGTGCGCTGATGGTCAACCACCATCACCAGCGGCGCTTTGGCGAGC
    GCGGCGTTCACGCGCGTAGCGCTTGCATGACGGTGGAGATCGTTTTCCAGCACCACCACCGCGTCCGCGC
    TGCCGGTTTCCAGCTCGCCCAACGCATCGTCAAGCGAACCGCCTCCCATCATACCCAGCCCCATGCTGTT
    CACGGAGCGGGCAATCATAGTAATACCGACGTCGGCGCCACGGCCTTTCAGCGCTTTCGCCACGTTGGCG
    GCCGCCTGAATCACCTCACTGCTACCGGCGTTAGTGCCGGAGATAATCAGCGGTTTTTTCGCGCCGGCCA
    ACGCCTGCACAATGACATCAATTTTGTTTTGCAAGTCGCTGTCGATACCGTCAACCGCAGGCGCTGTATT
    GTCCAGCGCATGGGCAATCGCAAAGCCCAGGCGCGCCTGATCTTCCACCGGCGCGCGATAGGTCCAGGCG
    GCGATATCATCCAGACGGGTGTCGTCCACGTTGGTCACGAACAGCGGATGTTTGGCACGCTGGCCGATGT
    TAAGGATGGCCGCAATCTGCCAGTCGGCCACTTTCTGCGCCGCCGCCATTTCACGCGCTTTACCTTTCAC
    CGCCTGACGTACCGCCAGCGCAACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAAGACCAGTACCGCG
    TCATAAGATTCGATTTCCCGCAGCGCCGGGGTGTAAATGCCGCCTTCACGCAGCACTTTCAGCGCCAGTT
    GCAGACGTTCCTGCTCGCCGCGCGCGATACCGGTGTAGAAGTTTTCCGCACCAACCAGTTCACGCAGCGC
    AAAGTTGCTCTCAATGCTGGCGCGTGGCGAACCGATACCGATCACTTTCTTCGACTGGCGCAGAATATCC
    GCCGCGCCCTGCATCGCCTGCTCGGCGTTGAGGGTGATGAAGTCGTCGCCACGGCGCTGAACCGGCTGAC
    GCGGACGGTCTTTCAGGTTCACATAGCCATAGCCGAAACGACCGCGGTCGCAGAGGAAGTAGTGGTTAAC
    GGTACCGTTGTAACGGTTTTCGATACGGCGTAATTCGCCATAGCGCTCGCCCGGGCTGATGTTACAGCCG
    ATGGAACACTGCTGGCAGATGCTCGGCGCAAACTGCATGTCCCATTTACGGTTGTAACGCTCGGAGTGCG
    TTTTATCGGTGAAGACGCCGGTCGGGCAAATCTCGACCAGGTTACCGGAGAATTCGCTTTCCAGCGTACC
    GTCTTCCGGACGCCCGAAGTAGACGTTGTCATGCGCGCCATAGACGCCCAGATCGGTGCCGTCGGCATAA
    TCTTTGTAGTAACGCACACAACGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAACGGCCCCA
    GATCCTGGTTGCGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAACTGTGACCCGTCATCACGGTCAT
    ATCCTGAAGATGGCAGTTACCGCCCTCTTCACAGACCGGACAGTCGTGCGGGTGGTTAGTCATCAGCCAC
    TCCACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATAGAGATAAAGGTACCGTCGGTGGCTG
    GCGTCATACAGGACATCACCAGGCGACCCCGCGTATCTTCCGCGTTTTGGTATTGCTTCACCGCACACTG
    GCGGCAAGCACCGACGCTTCCCAGCGCCGGGTGCCAGCAAAAATACGGAATATCAAGGCCGAGGGACAGA
    CATGCCTGTAGCAGGTTGTCCGCGCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATCGTAGCCA
  • Protein Sequence : Show Sequence
    >gi|205353438|ref|YP_002227239.1| NADH dehydrogenase subunit G [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
    MATIHVDGKEYEVNGADNLLQACLSLGLDIPYFCWHPALGSVGACRQCAVKQYQNAEDTRGRLVMSCMTP
    ATDGTFISIDDEEAKQFRESVVEWLMTNHPHDCPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDL
    GPFISHEMNRCIACYRCVRYYKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKT
    HSERYNRKWDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLKDRPR
    QPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASIESNFALRELVGAENFYTGIARGEQERLQ
    LALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALAVRQAVKGKAREMAAAQKVADWQIAAILN
    IGQRAKHPLFVTNVDDTRLDDIAAWTYRAPVEDQARLGFAIAHALDNTAPAVDGIDSDLQNKIDVIVQAL
    AGAKKPLIISGTNAGSSEVIQAAANVAKALKGRGADVGITMIARSVNSMGLGMMGGGSLDDALGELETGS
    ADAVVVLENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNEGRAQRF
    FQVYDPAYYDNKTIMLESWRWLHSLHSTVENREVDWTQLDHVIDAVIAAMPQFAGIKDAAPDATFRIRGQ
    KLAREPHRYSGRTAMRANISVHEPRQPQDKDTMFAFSMEGNNQPTAPRSEIPFAWAPGWNSPQAWNKFQD
    EVGGKLRHGDPGVRLIEATEGGLDYFTTVPASFQAQDGQWRIAPYYHLFGSDGLSQRSPVFQSRMPQPYI
    KLNPADAAKLGVNAGTRVSFSYDGNTVTLPVEISEGLAAGQVGLPMGMPGIAPVLAGARLEDLREAQQ
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A nuoG mutant is attenuated in chickens and induced significant protection from challenge with wild type S. Gallinarum (Zhang-Barber et al., 1998).
  • Related Vaccine(s): Salmonella Gallinarum nuoG mutant vaccine
42. ompR
  • Gene Name : ompR
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
  • NCBI Protein GI : 301160045
  • Other Database IDs : CDD:181883
    CDD:29071
    CDD:29475
    UniProtKB/TrEMBL: E1WIS9
  • Taxonomy ID : 216597
  • Gene Strand (Orientation) : ?
  • Protein Name : two-component response regulator OmpR
  • Protein Length : 239
  • Protein Note : osmolarity response regulator; Provisional; PRK09468
  • Protein Sequence : Show Sequence
    >gi|301160045|emb|CBW19564.1| two-component response regulator OmpR [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
    MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLLTRESFHLMVLDLMLPGEDGLSICRRL
    RSQSNPMPIIMVTAKGEEVDRIVGLEIGADDYIPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAF
    GKFKLNLGTREMFREDEPMPLTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRR
    MVEEDPAHPRYIQTVWGLGYVFVPDGSKA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An ompR mutant is attenuated in mice after oral challenge. Twenty eight days after immunization, mice were challenged with wild type S. typhimurium and were significantly protected (Dorman et al., 1989).
  • Related Vaccine(s): Salmonella Typhimurium ompR mutant vaccine
43. PhoP
  • Gene Name : PhoP
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. Ty2
  • NCBI Gene ID : 1071307
  • NCBI Protein GI : 29142123
  • Locus Tag : t1689
  • Genbank Accession : AE014613
  • Protein Accession : NP_805465
  • Taxonomy ID : 209261
  • Gene Starting Position : 1752334
  • Gene Ending Position : 1753008
  • Gene Strand (Orientation) : +
  • Protein Name : DNA-binding transcriptional regulator PhoP
  • Protein pI : 5.16
  • Protein Weight : 23440.94
  • Protein Length : 224
  • Protein Note : response regulator in two-component regulatory system with PhoQ; involved in magnesium starvation and stress
  • DNA Sequence : Show Sequence
    >gi|29140543:1752334-1753008 Salmonella enterica subsp. enterica serovar Typhi str. Ty2, complete genome
    GATGATGCGCGTACTGGTTGTAGAGGATAATGCATTATTACGCCACCACCTGAAGGTTCAGCTCCAGGAT
    TCAGGTCACCAGGTCGATGCCGCAGAAGATGCCAGGGAAGCTGATTACTACCTTAATGAACACCTTCCGG
    ATATCGCTATTGTCGATTTAGGTCTGCCGGATGAAGACGGCCTTTCCTTAATACGCCGCTGGCGCAGCAG
    TGATGTTTCACTGCCGGTTCTGGTGTTAACCGCGCGCGAAGGCTGGCAGGATAAAGTCGAGGTTCTCAGC
    TCCGGGGCCGATGACTACGTGACGAAGCCATTCCACATCGAAGAGGTAATGGCGCGTATGCAGGCGTTAA
    TGCGCCGTAATAGCGGTCTGGCCTCCCAGGTGATCAACATCTCGCCTTTCCAGGTGGATCTCTCACGCCG
    GGAATTATCCGTCAATGAAGAGGTCATCAAACTCACGGCGTTCGAATACACCATTATGGAAACGCTTATC
    CGTAACAACGGTAAAGTGGTCAGCAAAGATTCTCTGATGCTTCAGCTGTATCCGGATGCGGAACTGCGGG
    AAAGTCATACCATTGATGTTCTCATGGGGCGTCTGCGGAAAAAAATACAGGCCCAGTATCCGCACGATGT
    CATTACCACCGTACGCGGACAAGGATATCTTTTTGAATTGCGCTA
  • Protein Sequence : Show Sequence
    >gi|29142123|ref|NP_805465.1| DNA-binding transcriptional regulator PhoP [Salmonella enterica subsp. enterica serovar Typhi str. Ty2]
    MMRVLVVEDNALLRHHLKVQLQDSGHQVDAAEDAREADYYLNEHLPDIAIVDLGLPDEDGLSLIRRWRSS
    DVSLPVLVLTAREGWQDKVEVLSSGADDYVTKPFHIEEVMARMQALMRRNSGLASQVINISPFQVDLSRR
    ELSVNEEVIKLTAFEYTIMETLIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPHDV
    ITTVRGQGYLFELR
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Three S. typhi deletion mutants (phoP, rpoS, and rpoS-phoP double mutant) were constructed and characterized with respect to invasiveness, virulence, and protective immune response compared with wild-type Ty2. All S. typhi mutants showed an increase in the specific serum IgG levels and T-cell-mediated immunity, and showed equal protection abilities against a wild-type Ty2 challenge after two rounds of immunization in BALB/c mice (Lee et al., 2007).
  • Related Vaccine(s): Salmonella Typhi phoP mutant vaccine
44. poxA
  • Gene Name : poxA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1255870
  • NCBI Protein GI : 16767593
  • Locus Tag : STM4344
  • Genbank Accession : AE006468
  • Protein Accession : NP_463208
  • 3D structure: PDB ID : 3G1Z
  • Taxonomy ID : 700720
  • Gene Starting Position : 4586094
  • Gene Ending Position : 4587071
  • Gene Strand (Orientation) : +
  • Protein Name : lysyl-tRNA synthetase
  • Protein pI : 4.92
  • Protein Weight : 35366.49
  • Protein Length : 325
  • Protein Note : lysine--tRNA ligase beta chain; poxA; class II aminoacyl tRNA synthetase; catalyzes a two-step reaction; first charging a lysine molecule by linking the carboxyl group to the alpha-phosphate of ATP; second by transfer of the aminoacyl-adenylate to its tRNA
  • DNA Sequence : Show Sequence
    >gi|16763390:4586094-4587071 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    CATGAGCGAAACGGCAACCTGGCAGCCGAGCGCGTCCATCCCCAATTTATTAAAACGTGCGGCGATTATG
    GCGGAAATCCGTCGTTTCTTTGCCGATCGTGGAGTGCTTGAGGTTGAGACGCCCTGCATGAGTCAGGCGA
    CGGTCACAGACATTCATCTGTTCCCGTTCGAAACGCGTTTCGTCGGACCTGGCCATTCCCAGGGGATCAA
    CCTCTATTTAATGACCAGTCCGGAATACCATATGAAACGCCTGCTGGCGGCAGGGTGCGGCCCGGTTTTC
    CAGCTATGCCGCAGTTTCCGTAATGAAGAGATGGGACGACATCATAATCCGGAATTCACTATGCTGGAGT
    GGTATCGCCCGCATTACGATATGTACCGCCTGATGAATGAAGTGGATGATTTGCTTCAGCAAGTGCTGGA
    TTGTCAACCTGCGGAAAGTCTCTCCTATCAACAGGCGTTTCAGCGCCATCTGGAGATTGACCCGTTATCA
    GCAGATAAAACGCAACTGCGTGAGGCGGCGGCAAAGCTTGATTTAAGCAATATCGCCGATACGGAAGAAG
    ACCGTGATACGTTGCTGCAACTGTTGTTCACGATGGGGGTTGAGCCGCATATAGGTAAAGAAAAGCCGAC
    CTTTATTTATCACTTTCCGGCAAGTCAGGCATCGCTGGCACAAATCAGTACCGAGGATCATCGCGTCGCC
    GAGCGCTTTGAGGTGTACTACAAAGGTATTGAGCTGGCGAATGGTTTCCACGAACTGACGGACGCACGTG
    AGCAACAACAGCGCTTTGAACAGGACAATCGTAAGCGCGCCGCTCGCGGTCTGCCGCAGCAGCCGATTGA
    CCAAAATCTACTGGATGCGCTGGCCGCCGGTCTACCGGATTGTTCCGGCGTGGCGCTGGGTGTTGATCGT
    CTGGTGATGCTGGCGCTGGGAGCAGAAAGCCTGGCGGACGTGATTGCTTTTACGGTCGATCGGGCGTA
  • Protein Sequence : Show Sequence
    >gi|16767593|ref|NP_463208.1| lysyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MSETATWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLFPFETRFVGPGHSQGIN
    LYLMTSPEYHMKRLLAAGCGPVFQLCRSFRNEEMGRHHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLD
    CQPAESLSYQQAFQRHLEIDPLSADKTQLREAAAKLDLSNIADTEEDRDTLLQLLFTMGVEPHIGKEKPT
    FIYHFPASQASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQQPID
    QNLLDALAAGLPDCSGVALGVDRLVMLALGAESLADVIAFTVDRA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A poxA mutant is attenuated in mice and induces significant protection from challenge with wild type S. typhimurium (Kaniga et al., 1998).
  • Related Vaccine(s): Salmonella Typhimurium poxA mutant vaccine
45. relA
  • Gene Name : relA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6922678
  • NCBI Protein GI : 205353902
  • Locus Tag : SG2866
  • Genbank Accession : AM933173
  • Protein Accession : YP_002227703
  • Taxonomy ID : 550538
  • Gene Starting Position : 2982005
  • Gene Ending Position : 2984239
  • Gene Strand (Orientation) : -
  • Protein Name : GDP/GTP pyrophosphokinase
  • Protein pI : 6.78
  • Protein Weight : 78259.88
  • Protein Length : 744
  • Protein Note : (p)ppGpp synthetase; catalyzes the formation of pppGpp and ppGpp from ATP and GTP or GDP
  • DNA Sequence : Show Sequence
    >gi|205351346:2982005-2984239 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
    TTTACCCCCCGTGCAGTCGCCGTGCATCAATCACATCCGGCACCTGGTTCAGCTTACCGAGCACGCGGCC
    CAGCACCTGCAGGTTGTAGATCTCGATGGTCATATCAATGGTGGCGATCTGCTGTTTAATGTCGCTGCGG
    CTGGCGACGCCCAGCACGTTGACTTTTTCGTTAGCCAGAATAGTGGTGATATCGCGCAGCAAGCCGCTGC
    GATCGTTGGCCTGGACGCGCACCACCAGCGAATATCCCGCCGAGTAGCTTTCGCCCCATACCGCCTCTAC
    GATCCGCTCCGGCGCATGGGAGCGCAGTTCCGCCAGCTGTTCGCAGTCGGCCCGGTGCACGGAAATTCCC
    CGCCCCTGAGTAATGAAGCCGACAATTTCATCGCCAGGAATCGGCTGGCAGCAGCGGGCAATGTGGTGCA
    TCAGGTTACCAACCCCTTCCACCACCACGCGCCCGTCGTCTTTACGACGATTTTGCGGCGCGTATGTTTT
    TTGCTGAAGCTGTTTCAGCGCCGCTGCATCCTGCTCCTCCGCACTCGGCTTATTGAACTGCGATTGCAGG
    AAATTCACCATCTGATTAAGACGGATATCGCCGCCGCCTATCGCCGCCAGCAACTCTTCCAGCTCATTAA
    AGTTGTAGCGCGGCAGCAGATGTTTTTCGGCCTCTTTCAGGCTAATCCCCAAATGCGCCAGCTCATCGTC
    GAGGATCTGCCGTCCAGCCTGGATATTTTTGTCACGATCCTGCTTGCGGAACCAGGCGTGAATTTTCGAG
    CGTCCGCGGCTGGTCGTCACATAGCCCAGGTTTGGATTCAGCCAGTCGCGGCTGGGATTCGGCTGCTTCT
    GAGTGATAATTTCAATTTGATCACCCATCTGCAACTGATAGGTGAATGGCACAATACGGCCGCCGATTTT
    AGCGCCAATGCAGCGGTGCCCAACATCGCTGTGGATGTGGTAAGCAAAATCGAGCGGCGTAGATCCGGCA
    GGCAAGTCAACCACGTCGCCTTTTGGCGTAAAAACGTAGACCCGATCGTCAAACACCTGGCTGCGCACTT
    CATCCAGCATTTCGCCGGAATCGGCCATCTCTTCCTGCCAGGCGATCAGCTTACGCAGCCACGCAATTCT
    GTCTTCATGACCGGAGCGCACGCCGCCGGACGCGGCGCCTTCTTTATACTTCCAGTGTGCCGCCACGCCC
    AGTTCGGCGTCTTCATGCATCTGTTTGGTACGGATCTGGATCTCAACGGTTTTACCGCCCGGTCCCAGGA
    CCACGGTGTGGATAGACTGGTAACCGTTCGGTTTCGGGTTAGCGACATAATCATCGAATTCATCCGGCAG
    GTGACGATAGTGCGTATGCACTATCCCCAACGCGGCGTAGCAGTCCTGCAGACGTTCAGCGACAATACGC
    ACGGCGCGCACGTCAAAGAGTTCATCAAACGCCAGATGCTTTTTCTGCATTTTGCGCCAGATGCTATAAA
    TATGTTTTGGTCGTCCGTAGACCTCCGCCTGCACGCCTTCGTTTTTCATTTCGGCGCGCAGATGTCCAAC
    AAACTCTTCGATGTAATGTTCGCGATCGAGACGGCGCTCATGCAGCAGTTTGGCGATGCGTTTGTATTCC
    GCCGGATGCAGGTAGCGGAAACAGTAGTCTTCCAGTTCCCACTTCAGTTGCCCGATGCCCAGACGATTAG
    CGAGCGGCGCATAGATGTTGGTACATTCTTTCGCCGCCAGCACGCGCTCATCTTCCGGCGCCTCTTTCAC
    TTCGCGCAAATGAGCGATTCGCTCGGCCAGTTTGATCACCACGCAGCGGAAATCATCCACCATCGCCAAT
    AACATTCGACGGACGTTATCAACCTGCTCCGAAGAAACAGAGTCGTTATGAGTGGCGTTTAGCTGACGGA
    TCGCCGCCATATCGCGCACGCCATGAATCAGGGTAACGATAGATTTGCCGACGCTTTCGCGCAGTACATC
    TTCGCTGACTACGTTGGCGTCGGCCAGAGGGAACAGTAGCGCCGCCCGCAGCGTGTCGATATCCATACTC
    AGCGTGGAAAGAATTTCTACCATCTCCACGCCACGCCACAGCAACAGATCCGCATCCGGATGTCCTTGTG
    TCTGTTGCAGGCAATACGCCCAGGTTTCGGCTAAGCGCTCACACGACTGCTGGCTGGAAATTCCCAGGCT
    TGCGATCCACTTCTTCGGATCAAATTCACCAGCTTTATTAATATGTGCACTTCTTACCGCGACCA
  • Protein Sequence : Show Sequence
    >gi|205353902|ref|YP_002227703.1| GDP/GTP pyrophosphokinase [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
    MVAVRSAHINKAGEFDPKKWIASLGISSQQSCERLAETWAYCLQQTQGHPDADLLLWRGVEMVEILSTLS
    MDIDTLRAALLFPLADANVVSEDVLRESVGKSIVTLIHGVRDMAAIRQLNATHNDSVSSEQVDNVRRMLL
    AMVDDFRCVVIKLAERIAHLREVKEAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPAE
    YKRIAKLLHERRLDREHYIEEFVGHLRAEMKNEGVQAEVYGRPKHIYSIWRKMQKKHLAFDELFDVRAVR
    IVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTVEIQIRTKQMHEDAELG
    VAAHWKYKEGAASGGVRSGHEDRIAWLRKLIAWQEEMADSGEMLDEVRSQVFDDRVYVFTPKGDVVDLPA
    GSTPLDFAYHIHSDVGHRCIGAKIGGRIVPFTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRS
    KIHAWFRKQDRDKNIQAGRQILDDELAHLGISLKEAEKHLLPRYNFNELEELLAAIGGGDIRLNQMVNFL
    QSQFNKPSAEEQDAAALKQLQQKTYAPQNRRKDDGRVVVEGVGNLMHHIARCCQPIPGDEIVGFITQGRG
    ISVHRADCEQLAELRSHAPERIVEAVWGESYSAGYSLVVRVQANDRSGLLRDITTILANEKVNVLGVASR
    SDIKQQIATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARRLHGG
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A ppGpp-defective Salmonella Gallinarum, which is attained by mutating the relA and spoT genes, is attenuated in chickens and induces protection from oral challenge with wild type S. Gallinarum (Park et al., 2010).
  • Related Vaccine(s): Salmonella Gallinarum relA/spoT mutant vaccine
46. rfc
  • Gene Name : rfc
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
  • NCBI Protein GI : 267993210
  • Other Database IDs : CDD:33116
    CDD:186638
  • Taxonomy ID : 588858
  • Gene Strand (Orientation) : ?
  • Protein Name : O-antigen polymerase
  • Protein Length : 407
  • Protein Note : O-antigen polymerase (SW:RFC_SALTY)
  • Protein Sequence : Show Sequence
    >gi|267993210|gb|ACY88095.1| O-antigen polymerase [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]
    MLIISYIALCLLFIVYLYTLSVRIEGKIINVMVPYLIITVPTLYVFEGIFVYLSEVQNYTVEYLFFYTCY
    ITYIASFVISYLYTQRKPIYNKSNTKNKPRYVFTSLLFTFLAFIIYLPVLMEFREYILSPRRIYELTRTG
    YGIYFYPSLMFSLVASICAFFTYKKSKLFCISIVLFNCILIFLHGNKGPIFSIFIAFILYLSYIENKKIK
    FMFLVKSFAVIAVIVTAFFAYTFTDGNPIENMANYSDYTRNAVLVASSNFDFMYGKLLMESEVYSRIPRA
    IWPDKPEDFGALYLAKVFFPDAFYRNQGAPAFGYGELYADFGLFTPVWLVISGVFKGVLAKYFSNKTQET
    KSAHYFIMFLFCIGISVIPVSMGWLFPEHLMIAFMVYIASSFVFSEHIRFVLLRNNK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An rfc mutant, in combination with an asd mutation, is attenuated in mice and induces significant protection from challenge with wild type S. typhimurium (Piao et al., 2010).
  • Related Vaccine(s): Salmonella Typhimurium asd/rfc mutant vaccine
47. rpoB
  • Gene Name : rpoB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1255679
  • NCBI Protein GI : 16767407
  • Locus Tag : STM4153
  • Genbank Accession : AF170176
  • Protein Accession : NP_463022
  • Taxonomy ID : 700720
  • Gene Starting Position : 4365907
  • Gene Ending Position : 4369935
  • Gene Strand (Orientation) : +
  • Protein Name : DNA-directed RNA polymerase subunit beta
  • Protein pI : 4.89
  • Protein Weight : 139687.68
  • Protein Length : 1342
  • Protein Note : DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; beta subunit is part of the catalytic core which binds with a sigma factor to produce the holoenzyme
  • DNA Sequence : Show Sequence
    >gi|16763390:4365907-4369935 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    TATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGAT
    GTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAGTTTATCGAGCAAGATCCTGAAGGGCAGT
    ACGGCCTGGAAGCAGCCTTCCGTTCCGTGTTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAGTA
    CGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTTCAGGAATGTCAGATCCGTGGCGTGACCTATTCC
    GCACCGCTGCGCGTAAAACTGCGTCTGGTGATCTACGAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACA
    TTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACCGACAACGGTACCTTTGTTATCAACGG
    TACTGAGCGTGTTATCGTTTCTCAGCTGCACCGTAGCCCTGGCGTCTTCTTTGACTCCGACAAAGGTAAA
    ACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGCATCATTCCTTACCGTGGTTCCTGGCTGGACTTCG
    AATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTACCTGCGACCATCATCCT
    GCGTGCGCTGAACTACACCACTGAGCAGATCCTTGACCTGTTCTTTGAGAAAGTCGTCTTCGAAATTCGC
    GACAACAAACTGCAGATGGAGCTGATTCCAGAACGTCTGCGTGGCGAAACCGCGTCGTTCGATATCGAAG
    CTAACGGCAAAGTGTACGTTGAAAAAGGCCGTCGCATTACCGCGCGTCACATCCGTCAGCTGGAAAAAGA
    CGATATCAAGCATATCGAAGTTCCGGTTGAGTACATCGCAGGTAAAGTCGTCTCTAAAGACTACGTTGAC
    GAATCTACTGGCGAGCTGATCTGCGCGGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCC
    AGTCCGGCCACAAGCGTATCGAAACGCTGTTTACCAACGATCTGGACCACGGCCCGTACATTTCTGAAAC
    GGTACGCGTCGACCCAACTAACGATCGTCTGAGCGCGCTGGTAGAAATCTACCGCATGATGCGCCCTGGT
    GAGCCGCCGACACGCGAAGCGGCTGAAAGCCTGTTTGAGAACCTGTTCTTCTCCGAAGACCGCTATGACC
    TGTCTGCGGTGGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGACGAAATCGAAGGTTCCGGTATCCT
    GAGCAAAGACGACATCATTGATGTCATGAAGAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTGGAT
    GATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTCGGCGAAATGGCGGAAAACCAGTTCCGCGTTG
    GCCTGGTGCGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCTCA
    GGATATGATCAACGCCAAGCCGATTTCCGCCGCAGTGAAAGAGTTCTTTGGTTCCAGCCAGCTGTCTCAG
    TTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCG
    GTCTGACCCGCGAACGCGCAGGCTTCGAAGTTCGAGACGTACACCCGACGCACTACGGTCGCGTATGTCC
    TATCGAAACGCCGGAAGGTCCGAACATCGGTCTGATCAACTCCCTGTCCGTGTACGCACAGACTAACGAA
    TATGGCTTCCTTGAGACGCCGTACCGTCGTGTGGTTGATGGCGTGGTTACTGACGAAATTCATTACCTGT
    CTGCTATCGAAGAAGGCAACTACGTTATCGCTCAGGCGAACTCCAACCTGGATGACGAAGGCCACTTTGT
    AGAAGATTTGGTGACCTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATG
    GACGTTTCCACCCAGCAGGTGGTCTCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCA
    ACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCGGTTCCGACTCTGCGCGCTGACAAGCCGCTGGT
    GGGTACCGGTATGGAACGTGCTGTCGCCGTCGACTCCGGTGTTACTGCAGTAGCTAAACGTGGCGGTACT
    GTTCAGTACGTGGATGCGTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTACCCGGGCGAAGCGG
    GTATCGACATCTATAACCTGACCAAATACACCCGCTCTAACCAGAACACCTGTATCAACCAGATGCCATG
    TGTGTCTCTGGGCGAGCCGGTTGAACGCGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAA
    CTGGCGCTCGGTCAGAACATGCGCGTGGCGTTCATGCCGTGGAACGGCTACAACTTCGAAGACTCCATTC
    TCGTTTCCGAGCGTGTTGTCCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGCGTGTC
    CCGTGACACCAAGCTGGGGCCGGAAGAGATCACCGCTGATATCCCGAACGTGGGTGAAGCTGCGCTCTCC
    AAACTGGATGAATCCGGTATCGTTTACATCGGCGCGGAAGTGACAGGCGGTGACATTCTGGTCGGTAAGG
    TGACGCCGAAAGGTGAAACCCAGCTGACGCCGGAAGAGAAACTGCTGCGTGCGATCTTCGGTGAGAAAGC
    GTCTGACGTTAAAGACTCTTCTCTGCGCGTACCTAACGGTGTCTCCGGTACGGTTATCGACGTTCAGGTC
    TTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCTCTGGAAATCGAAGAGATGCAGCTCAAGCAGGCGA
    AAAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGCCTGTTTAGCCGTATCCGTGCGGTGCTGGT
    TTCCAGCGGCGTTGAAGCTGAGAAGCTCGACAAACTGCCGCGCGACCGCTGGCTGGAACTCGGTCTGACC
    GACGAAGAGAAACAAAATCAGCTGGAACAACTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGA
    AAAAACTTGAAGCGAAGCGCCGTAAAATCACCCAGGGCGACGATCTGGCGCCGGGCGTGCTGAAGATCGT
    GAAGGTCTATCTGGCCGTTAAACGCCGTATCCAGCCGGGTGATAAGATGGCAGGTCGTCACGGTAACAAG
    GGTGTTATCTCTAAGATCAACCCGATCGAAGACATGCCTTACGATGAAAACGGCACGCCGGTAGACATCG
    TACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGATTCTGGAAACCCACCTGGGTATGGC
    TGCGAAAGGTATTGGCGACAAGATTAACGCCATGCTGAAACAGCAGCAGGAAGTCGCGAAACTGCGCGAA
    TTCATCCAGCGTGCCTACGATCTGGGCGCCGACGTTCGTCAGAAAGTCGACCTGAGCACCTTCAGCGATG
    ATGAAGTGCTGCGTCTGGCAGAAAACCTGCGCAAAGGTATGCCGATTGCCACACCAGTGTTTGACGGTGC
    GAAAGAAGCGGAAATCAAAGAGCTGCTGAAACTGGGCGACCTGCCGACCTCCGGTCAGATCACCCTGTTC
    GATGGCCGTACGGGTGAACAGTTCGAACGTCCGGTAACCGTCGGTTACATGTACATGCTGAAACTGAACC
    ACCTGGTTGATGACAAGATGCATGCGCGTTCTACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGG
    TGGTAAGGCGCAGTTCGGTGGTCAGCGCTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCTTACGGCGCC
    GCCTACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAATGGTCGTACCAAGATGTATAAGA
    ACATCGTGGACGGCAACCATCAGATGGAACCGGGTATGCCGGAATCCTTCAACGTTCTGTTGAAAGAGAT
    CCGCTCGCTGGGCATCAACATCGAACTGGAAGACGAGTA
  • Protein Sequence : Show Sequence
    >gi|16767407|ref|NP_463022.1| DNA-directed RNA polymerase subunit beta [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQSYSGNSELQY
    VSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVING
    TERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIIL
    RALNYTTEQILDLFFEKVVFEIRDNKLQMELIPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKD
    DIKHIEVPVEYIAGKVVSKDYVDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISET
    VRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLRDEIEGSGIL
    SKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQ
    DMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCP
    IETPEGPNIGLINSLSVYAQTNEYGFLETPYRRVVDGVVTDEIHYLSAIEEGNYVIAQANSNLDDEGHFV
    EDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLV
    GTGMERAVAVDSGVTAVAKRGGTVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPC
    VSLGEPVERGDVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVS
    RDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKA
    SDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLFSRIRAVLV
    SSGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGDDLAPGVLKIV
    KVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMA
    AKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDDEVLRLAENLRKGMPIATPVFDGA
    KEAEIKELLKLGDLPTSGQITLFDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLG
    GKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMPESFNVLLKEI
    RSLGINIELEDE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A gyrA-cpxA-rpoB Salmonella Typhimurium mutant was attenuated and protected against challenge in pigs (Roesler et al., 2004).
  • Related Vaccine(s): Salmonella Typhimurium gyrA/cpxA/rpoB mutant vaccine
48. rpoS
  • Gene Name : rpoS
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium SL1344
  • NCBI Protein GI : 301159483
  • Other Database IDs : CDD:180185
    CDD:189414
    CDD:146937
    CDD:146934
    CDD:100119
    UniProtKB/TrEMBL: E1WAG8
  • Taxonomy ID : 216597
  • Gene Strand (Orientation) : ?
  • Protein Name : RNA polymerase sigma subunit RpoS (sigma-38)
  • Protein Length : 330
  • Protein Note : RNA polymerase sigma factor RpoS; Validated
  • Protein Sequence : Show Sequence
    >gi|301159483|emb|CBW19002.1| RNA polymerase sigma subunit RpoS (sigma-38) [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
    MSQNTLKVHDLNEDAEFDENGVEAFDEKALSEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPL
    LTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGF
    RFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVS
    RMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGL
    LGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Immunizations with Salmonella typhimurium rpoS and rpoS aroA mutants protected susceptible BALB/c mice against subsequent oral challenge with virulent S. typhimurium. When administered orally or intraperitoneally, rpoS derivatives of the mouse-virulent S. typhimurium strains, C52 and SL1344, were highly attenuated and were efficient single-dose live vaccines (Coynault et al., 1996).
  • Related Vaccine(s): Salmonella Typhimurium rpoS mutant vaccine
49. ruvB
  • Gene Name : ruvB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1253415
  • NCBI Protein GI : 16765236
  • Locus Tag : STM1894
  • Genbank Accession : AE006468
  • Protein Accession : NP_460851
  • Taxonomy ID : 700720
  • Gene Starting Position : 1989053
  • Gene Ending Position : 1990063
  • Gene Strand (Orientation) : -
  • Protein Name : Holliday junction DNA helicase RuvB
  • Protein pI : 5.03
  • Protein Weight : 34934.59
  • Protein Length : 336
  • Protein Note : promotes strand exchange during homologous recombination; RuvAB complex promotes branch migration; RuvABC complex scans the DNA during branch migration and resolves Holliday junctions at consensus sequences; forms hexameric rings around opposite DNA arms; requires ATP for branch migration and orientation of RuvAB complex determines direction of migration
  • DNA Sequence : Show Sequence
    >gi|16763390:1989053-1990063 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    GCTAAGGCATCTCTGGCGGCGTGATCCCGAAATGGTTCCAGGCGCGCACCGTTGCCATACGCCCACGCGG
    CGTACGCTGCAAAAAGCCTTGCTGAATCAGATACGGCTCCAGCACATCCTCAATGGTTTCACGCTCTTCG
    CCAATCGCCGCCGCCAGGTTATCCAGTCCCACCGGACCACCGAAGAATTTATCAATAACCGCCAGCAGCA
    GCTTGCGATCCATATAGTCAAACCCTTCCGCATCGACGTTCAGCATATCCAGCGCCTGCGCGGCAATTTC
    CGCTGAAATAGCCCCGTCATGCTTCACCTCAGCAAAATCACGCACACGTCTGAGCAGACGGTTGGCTATA
    CGCGGCGTACCGCGCGCCCGACGCGCCACTTCCAGCGCGCCGTCATCGCTCATCTCAAGCCCCATGTGCC
    TTGCGCTGCGCCCAACGATGTGCTGCAGATCGGGCACCTGGTAGAACTCCAGGCGCTGCACAATACCAAA
    ACGGTCACGTAGCGGGGACGTTAACGACCCGGCGCGCGTCGTCGCGCCAATGAGCGTAAACGGCGGCAGA
    TCGATCTTAATTGAACGTGCGGCAGGCCCTTCGCCAATCATAATATCCAACTGGTAATCTTCCATCGCCG
    GATAGAGTACCTCTTCCACCACCGGTGAAAGCCGGTGAATCTCATCAATAAACAGCACATCATGCGGTTC
    AAGATTGGTGAGCATAGCCGCCAGATCGCCTGCTTTTTCCAGCACCGGGCCGGACGTGGTTCGGAGATTG
    ACGCCCATTTCATTGGCAACGATATTCGCCAGAGTCGTTTTACCTAACCCTGGCGGGCCGAAAATCAGCA
    AGTGATCTAGTGCGTCGCCACGCCGTTTTGCCGCCTGAATAAAGATCTCCATCTGGGAGCGCACCTGCGG
    TTGGCCGACATATTCCGCCAATAACTTCGGGCGAATGGCGCGATCGGCGACGTCTTCTGCGATCGTGGCG
    CCTGCTGAAATCAGGCGATCTGCTTCTATCA
  • Protein Sequence : Show Sequence
    >gi|16765236|ref|NP_460851.1| Holliday junction DNA helicase RuvB [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MIEADRLISAGATIAEDVADRAIRPKLLAEYVGQPQVRSQMEIFIQAAKRRGDALDHLLIFGPPGLGKTT
    LANIVANEMGVNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIG
    EGPAARSIKIDLPPFTLIGATTRAGSLTSPLRDRFGIVQRLEFYQVPDLQHIVGRSARHMGLEMSDDGAL
    EVARRARGTPRIANRLLRRVRDFAEVKHDGAISAEIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGP
    VGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPEMP
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A ruvB mutant is attenuated in mice and induced significant protection from challenge with wild type S. Typhimurium (Choi et al., 2010).
  • Related Vaccine(s): Salmonella Typhimurium ruvB mutant vaccine
50. SopB
  • Gene Name : SopB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • VO ID : VO_0010999
  • NCBI Protein GI : 54036439
  • Other Database IDs : CDD:147852
  • Taxonomy ID : 90371
  • Gene Strand (Orientation) : ?
  • Protein Name : Inositol phosphate phosphatase sopB
  • Protein Length : 561
  • Protein Note : Effector protein sopB
  • Protein Sequence : Show Sequence
    >gi|54036439|sp|O30916.2|SOPB_SALTY RecName: Full=Inositol phosphate phosphatase sopB; AltName: Full=Effector protein sopB
    MQIQSFYHSASLKTQEAFKSLQKTLYNGMQILSGQGKAPAKAPDARPEIIVLREPGATWGNYLQHQKASN
    HSLHNLYNLQRDLLTVAATVLGKQDPVLTSMANQMELAKVKADRPATKQEEAAAKALKKNLIELIAARTQ
    QQDGLPAKEAHRFAAVAFRDAQVKQLNNQPWQTIKNTLTHNGHHYTNTQLPAAEMKIGAKDIFPSAYEGK
    GVCSWDTKNIHHANNLWMSTVSVHEDGKDKTLFCGIRHGVLSPYHEKDPLLRHVGAENKAKEVLTAALFS
    KPELLNKALAGEAVSLKLVSVGLLTASNIFGKEGTMVEDQMRAWQSLTQPGKMIHLKIRNKDGDLQTVKI
    KPDVAAFNVGVNELALKLGFGLKASDSYNAEALHQLLGNDLRPEARPGGWVGEWLAQYPDNYEVVNTLAR
    QIKDIWKNNQHHKDGGEPYKLAQRLAMLAHEIDAVPAWNCKSGKDRTGMMDSEIKREIISLHQTHMLSAP
    GSLPDSGGQKIFQKVLLNSGNLEIQKQNTGGAGNKVMKNLSPEVLNLSYQKRVGDENIWQSVKGISSLIT
    S
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : The entire SopB and SifB genes were cloned into DNA vaccine vectors and were administered along with live attenuated Salmonella. It was found that SopB vaccination reduced the bacterial burden of organs by about 5-fold on day 4 and day 8 after challenge with virulent Salmonella and proved to be a more efficient vaccination strategy than live attenuated bacteria alone (Nagarajan et al., 2009).
  • Related Vaccine(s): Salmonella DNA vaccine encoding SopB Protein
51. spoT
  • Gene Name : spoT
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6924903
  • NCBI Protein GI : 205354656
  • Locus Tag : SG3689
  • Genbank Accession : AM933173
  • Protein Accession : YP_002228457
  • Taxonomy ID : 550538
  • Gene Starting Position : 3881550
  • Gene Ending Position : 3883661
  • Gene Strand (Orientation) : -
  • Protein Name : bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase
  • Protein pI : 8.89
  • Protein Weight : 74653.38
  • Protein Length : 703
  • Protein Note : 3.1.7.2
  • DNA Sequence : Show Sequence
    >gi|205351346:3881550-3883661 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
    GCTAGTTTCGGTTACGGGTGACTTTAATAACGTCTGGCATCACGCGGATTTTGCGCATGATATTCGCCAG
    ATGTACGCGATCGCGTGCGGTAAGGCGAATAAAGGTACTATAGACGCGACCATCTTTCTCTTCAGTATTC
    AGGCTTTGAATATTGGAGGTGGTGGTATTAATCGCCGCCGTCAGGTTAGCCAGCGCGCCCTGATGGTTAA
    ACATTTCCACCTTGATTTCGGTAATGAATTCCTGCTCCGTCTCTTTGTCCCATTCGACCGCCATAAATTT
    CTCTGGCTCTTTCTGGTATCCACGGATATTACGGCAGGATTCGTGGTGGATCACCAGTCCTTTACCTGGG
    CTGACGTGAGCGATGATCGGGTCGCCTGGGATCGGACGACAACACTTCGCAAAGGTGATAAGCACGCCAT
    CCGCGCCTTTAATCGGCAGGTGGCCGTGATTCGATTGCGCAACGGTCGGCACCACGGCTTCGCCTTGCTG
    CAGATTTTTCGCGACCACTACGCTCATCGCGTTGCCGAGACCGATTTCCGCCAGCAGATCGTCAAGCGTT
    GCCAGCTTCATACGATCCAGTTCGCGCTGAATATTTTCCTGCGGAATTTCCGCCAGCTTACGACTGCCGC
    CTAAAGCATGGTTAAGCAGACGACGGCCCAGGCTTACGGAGTCATCACGTTTGAGGTTTTTCAGCAACTG
    ACGAATTTTAGCGCGCGCTTTAGAGCTGACGACAAAGTTCAGCCAGGCGGCGTTGGGACGCGCGCCCGGC
    GCGGTAATAATTTCGACGGTCTGACCGCTGCTAAGCGGCTGCGACAGCGGATAAGGCTGGCGGTCGACAC
    GCGCGCCGACGCAGGCGTGGCCGATGTCGGTATGCACTGCATAGGCAAAATCCACCGGCGTAGCGCCAGC
    GGGCAGTTCGACAATGCGCCCTTCCGGGGTGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTTACG
    CTTTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGTAGCTCCAGCAGGCTCTGCATCCAGCGCTGGG
    CGCGGATCTGCGCCGTGGTGCTGGTCTCACCGTGTTCTTTATACGCCCAGTGTGCCGCGACCCCCATTTC
    CGCCATCTGATCCATATCTTCGGTACGGATCTGGACTTCAACAGGAACGCCGTGCGGGCCGATCATCGAG
    GTGTGCAAAGACTGATAGCCGTTCGCTTTGGGAATGGCTATATAGTCTTTCACCCGTCCCGGACGCGGCT
    TATAGAGACTGTGCATCTGGCCGAGTACGCGATAGCAGGTATCGGAGTCATGAACGATCACGCGAAACGC
    GTAAATGTCCATGATCGAGTGAAAACGCTGCTCTTTGAGCACCATTTTACAGTAGATCGAGTAAAGATGT
    TTTTCGCGACCGCTAACGCGACACGGAATTCCCGCCTCTTGCAAGCGTCCTTCGATTTCAGAGAGGATTT
    TTTGGATCATCTCTTTACGGTTGCCGCGCGCCGCTTTTACCACTTCTTTAATGACGCGGTAACGATTGGG
    ATACAGCGCTTCAAAACCCAGCTCTTCCAGCTCGGTTTTAATGTGATGAATACCTAAACGGTGCGCCAGA
    GGACTGTAGATTTCCAGCGTTTCACGGGCAATACGACGACGTTTATCCGGGCGTAACGAGCCCAGCGTGC
    GCATGTTATGGGTACGGTCAGCAAGCTTAATGAGAATGACGCGGATATCCTGCACCATCGCCATAATCAT
    TTTGCGAAAGTTTTCGGCCTGCGCCTCTTTCTTATCGCGGAACTTGAGCTTATCAAGTTTCGACACCCCC
    TCTACCAGCTCGGCAACGCTTTTACCGAAAAGCTGTTCCATGTCCTGATAGGTGGCGGGGGTATCTTCAA
    TCACGTCATGCAGCAGAGCGGCCATCAGCGTTTCGTAGTCGAGTTTCATCTCGGCCAGAATACAGGCCAC
    CGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTCTGGCCCTCGTGAGCGTCACGTGCAACG
    AGATACGCCTGCCGAAGACGCTTAATCTGGTCTTCCGGCAGGTAGGTTTGAATCAGTTGATTCAGGCTTT
    CAAACAGATACA
  • Protein Sequence : Show Sequence
    >gi|205354656|ref|YP_002228457.1| bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
    MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAAL
    LHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADR
    THNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRK
    EMIQKILSEIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVHDSDTCYRVLGQM
    HSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEHGETSTT
    AQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIGHA
    CVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLL
    NHALGGSRKLAEIPQENIQRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQQGEAVVPTVAQSNHGHLP
    IKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETEQEFITEI
    KVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSTFIRLTARDRVHLANIMRKIRVMPDVIKVTR
    NRN
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A ppGpp-defective Salmonella Gallinarum, which is attained by mutating the relA and spoT genes, is attenuated in chickens and induces protection from oral challenge with wild type S. Gallinarum (Park et al., 2010).
  • Related Vaccine(s): Salmonella Gallinarum relA/spoT mutant vaccine
52. surA
  • Gene Name : surA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1251610
  • NCBI Protein GI : 16763482
  • Locus Tag : STM0092
  • Genbank Accession : AE006468
  • Protein Accession : NP_459097
  • Taxonomy ID : 700720
  • Gene Starting Position : 104469
  • Gene Ending Position : 105755
  • Gene Strand (Orientation) : -
  • Protein Name : peptidyl-prolyl cis-trans isomerase SurA
  • Protein pI : 7.32
  • Protein Weight : 44868.68
  • Protein Length : 428
  • Protein Note : Chaperone involved in the folding of extracytoplasmic proteins, especially OmpA, OmpF and LamB
  • DNA Sequence : Show Sequence
    >gi|16763390:104469-105755 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    ATTAGTTACTCAAAATCTTAACGTAAGCGCTGGCGCGCTGTTCTTGCATCCAGGTCGCCGCTTCTTCTGA
    GAATTTACGGTTCATCAGCATACGATAAGCGCGATCTTTCTGCGCCGCATCGGTTTTGTCTACCTTACGC
    GTATCCAGCAATTCGATCAGATGCCAGCCGAAAGAGGAGTGTACCGGCGCGCTTATTTGGCCTTTATGCA
    GCTTCGTTAGCGCGTCGCGGAACGCCGGGTCGAAAATATCTGGCGTAGCCCAACCCAAATCACCGCCCTG
    GTTAGCGGAGCCCGGGTCCTGAGAGTACTCTTTCGCCGCAGCGGCAAAGGTGGTTTTACCACTCTTAATG
    TCAGCCGCGATTTCTTCCAGCTTCAGGCGCGCCTGCTGATCGTTCATGATCGGCGACGGCTTAAGCAGAA
    TGTGACGAGCGTGAACTTCGGTCACGGAGATACTCTGGCTCTGACCGCGCAGGTCATTTACTTTCAGAAT
    GTGGAAGCCGACGCCGGAGCGAATCGGGCCGACAATGTCGCCTTTCTTCGCGGTGCTCAGCGCCTGGGCG
    AAAATCCCCGGCAGCTCCTGGATACGGCCCCAGCCCATCTGACCGCCTTTTAGCGCCTGCTGGTCGGCAG
    AGTAGGTAATCGCCAGTTTGCCGAAATCTGCGCCGTTACGCGCTTCTTCAACAATGCTTTCCGCCTGGCG
    CTGCGCGTCGTTAACCTGCTCGGAGGTTGGGTTTTCCGGCAGAGCAATCAGGATATGGCTCAGGTTCAGC
    TCGGTGCTGGCATCGTTTTGGGTGCCAATCTGTTTTGCCAGCGCGTCAACTTCTTGCGGCAAAACGGTGA
    TACGGCGACGAACCTCATTGTTGCGCACTTCAGAGATAATCATCTCTTTACGAATCTGGTTACGGTAGGT
    TGAATAGTTCAGCCCATCGTAAGCCAGACGGCTGCGCATCTGATCCATCGTCATATTGTTTTGTTTGGCG
    ATGTTGGCGATGGCCTGATCCAACTGCTCATCCGTGATCTTCACCCCCATCTTCTGACCCATCTGCAGGA
    TAATTTGATCCATAATCAAACGTTCCAGGATCTGGTGACGCAGCGTGGCGTCGTCCGGAAGCTGCTGACC
    TGCCTGACCCGCGTTGAGTTTGACTGATTGCATTAAGCCATCAACGTCGCTTTCCAGCACGACGCCATTA
    TTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATAC
    CGAGAAGCAGCGTTTTCCAGTTCTTCA
  • Protein Sequence : Show Sequence
    >gi|16763482|ref|NP_459097.1| peptidyl-prolyl cis-trans isomerase SurA [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDGLMQSVKLNAGQAGQQLPDDATLRHQI
    LERLIMDQIILQMGQKMGVKITDEQLDQAIANIAKQNNMTMDQMRSRLAYDGLNYSTYRNQIRKEMIISE
    VRNNEVRRRITVLPQEVDALAKQIGTQNDASTELNLSHILIALPENPTSEQVNDAQRQAESIVEEARNGA
    DFGKLAITYSADQQALKGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGQSQSI
    SVTEVHARHILLKPSPIMNDQQARLKLEEIAADIKSGKTTFAAAAKEYSQDPGSANQGGDLGWATPDIFD
    PAFRDALTKLHKGQISAPVHSSFGWHLIELLDTRKVDKTDAAQKDRAYRMLMNRKFSEEAATWMQEQRAS
    AYVKILSN
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A surA mutant (BRD1115) is attenuated in BALB/c mice and induces protection from challenge with wild type S. typhimurium (Sydenham et al., 2000).
  • Related Vaccine(s): Salmonella Typhimurium surA mutant vaccine
53. tolA
  • Gene Name : tolA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium SL1344
  • NCBI Protein GI : 301157338
  • Locus Tag : STM0747
  • Genbank Accession : AE006468
  • Protein Accession : NP_459732
  • Other Database IDs : CDD:181920
    CDD:200776
    UniProtKB/TrEMBL: E1W9P0
  • Taxonomy ID : 216597
  • Gene Starting Position : 814616
  • Gene Ending Position : 815839
  • Gene Strand (Orientation) : +
  • Protein Name : tolA protein
  • Protein pI : 9.79
  • Protein Weight : 40772.04
  • Protein Length : 407
  • Protein Note : cell envelope integrity inner membrane protein TolA; Provisional; PRK09510
  • DNA Sequence : Show Sequence
    >gi|16763390:814616-815839 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    AGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAACGGGCGATAATTATTTCAGCCGTGCTGCATATC
    ATCTTATTTGCAGTGCTGATCTGGAGTTCGTTTGATGAGCATATAGAGGCTTCTGCCGGCGGCGGCGGTG
    GTTCCGCTATCGACGCGGTGATGGTCGATCCTGGCGCCGTTGTGCAGCAGTACAACCGTCAGCAGGATCA
    ACAGGCCAGCGCCAGACGCGCGGAAGAAGAGCGTAAAAAGCTGCAACAGCAGCAAGCAGAGGAGCTGCAG
    CAGAAGCAGGCTGCTGAACAGGAGCGGTTGAAACAACTTGAGAAAGAACGTTTAGCGGCTCAGGAGCAGC
    AAAAGCAGGCTGAAGAAGCGGCAAAACTGGCGCAACAGCAGCAGCAACAGGCCGAAGAAGCGGCGAAAGC
    GGCGGCGGACGCGAAGAAAAAAGCGGAGGCCGAGGCGGCGAAAGCGGCGGCGGACGCGAAGAAGAAAGCG
    GAAGCCGAAGCGGTAAAAGCGGCAGCGGACGCGAAGAAGAAAGCGGAAGCCGAAGCGGCGAAAGCGGCGG
    CGGACGCGAAGAAGAAAGCAGAGGCCGAAGCGGCGAAAGCGGCGGCGGAGGCGAAGAAGAAAGCGGAAGC
    CGAAGCGGCGAAAGCGGCGGCGGAGGCGAAGAAGAAAGCGGATGCCGAGGCGGCGAAAGCGGCGGCGGAG
    GCGAAGAAGAAAGCGGATGCCGCGGCGGCGAAAGCGGCGGCGGAGGCGAAGAAGAAAGCGGATGCCGCGG
    CGGCGAAAGCAGCGGCGGACGCTAAGAAGAAAGCGGCTGCCGAAAAAGCGGCCGCCGCAGAAGGCGTCGA
    CGATCTGCTTGGCGATCTCAGCTCGGGTAAGAATGCGCCGAAAACCGGCGGCGGCGCGAAAGGGAATGGT
    CAGCCATCGAAAGATAGCGGTACATCGGGCGCTAACGGTGGGGCGACAGGCGCTGATATCAGCGCCTACG
    CGAAACAGATTCAGGTCGCCATTCAGAGCCGTCTGTACGATGCGAGCCTGTATCAGGGCAAACAATGTGT
    CTTGCATATTAGCCTGGCGCCGGATGGCTCATTAAAAAGCATTACGTCTGAGGGCGGCGATCCGGCGCTT
    TGTCAGGCGGCGTTAATGGCGGCAAAAACCGCGAAAATTCCTAAACCGCCAAGCCAGGCTGTTTATGAGA
    AAATAAAGGATGCCAAACTAGACTTTAAACTGTA
  • Protein Sequence : Show Sequence
    >gi|301157338|emb|CBW16827.1| tolA protein [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
    MSKATEQNDKLKRAIIISAVLHIILFAVLIWSSFDEHIEASAGGGGGSAIDAVMVDPGAVVQQYNRQQDQ
    QASARRAEEERKKLQQQQAEELQQKQAAEQERLKQLEKERLAAQEQQKQAEEAAKLAQQQQQQAEEAAKA
    AADAKKKAEAEAAKAAADAKKKAEAEAVKAAADAKKKAEAEAAKAAADAKKKAEAEAAKAAAEAKKKAEA
    EAAKAAAEAKKKADAEAAKAAAEAKKKADAAAAKAAAEAKKKADAAAAKAAADAKKKAAAEKAAAAEGVD
    DLLGDLSSGKNAPKTGGGAKGNGQPSKDSGTSGANGGATGADISAYAKQIQVAIQSRLYDASLYQGKQCV
    LHISLAPDGSLKSITSEGGDPALCQAALMAAKTAKIPKPPSQAVYEKIKDAKLDFKL
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A tolA mutant is attenuated in mice and induced significant protection from challenge with wild type S. Typhimurium (Paterson et al., 2009).
  • Related Vaccine(s): Salmonella Typhimurium tolA mutant vaccine
54. trxA
  • Gene Name : trxA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium SL1344
  • NCBI Protein GI : 301160436
  • Locus Tag : STM3915
  • Genbank Accession : AE006468
  • Protein Accession : NP_462806
  • Other Database IDs : CDD:48496
    UniProtKB/TrEMBL: E1WDY2
  • Taxonomy ID : 216597
  • Gene Starting Position : 4124835
  • Gene Ending Position : 4125164
  • Gene Strand (Orientation) : +
  • Protein Name : thioredoxin
  • Protein pI : 4.46
  • Protein Weight : 11310.13
  • Protein Length : 109
  • Protein Note : TRX family; composed of two groups: Group I, which includes proteins that exclusively encode a TRX domain; and Group II, which are composed of fusion proteins of TRX and additional domains. Group I TRX is a small ancient protein that alter the redox...; cd02947
  • DNA Sequence : Show Sequence
    >gi|16763390:4124835-4125164 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    TATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCT
    ATCCTCGTTGATTTCTGGGCAGAGTGGTGCGGGCCGTGTAAAATGATCGCTCCGATTCTGGATGAAATCG
    CTGACGAATATCAGGGCAAATTGACCGTTGCCAAACTGAACATTGACCAGAACCCAGGTACTGCGCCTAA
    ATATGGCATCCGCGGTATTCCGACTCTGCTGCTGTTTAAAAACGGCGAAGTGGCGGCAACCAAAGTAGGC
    GCACTGTCTAAAGGTCAGTTGAAAGAGTTTCTCGACGCCAATCTGGCGTA
  • Protein Sequence : Show Sequence
    >gi|301160436|emb|CBW19965.1| thioredoxin [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
    MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPK
    YGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A trxA mutant is attenuated in mice and induced significant protection from challenge with wild type S. Typhimurium (Peters et al., 2010).
  • Related Vaccine(s): Salmonella Typhimurium trxA mutant vaccine
55. TviA
  • Gene Name : TviA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. CT18
  • NCBI Gene ID : 1250863
  • NCBI Protein GI : 16763124
  • Locus Tag : STY4662
  • Genbank Accession : NC_003198.1
  • Protein Accession : NP_458741.1
  • Taxonomy ID : 28901
  • Gene Starting Position : 4524140
  • Gene Ending Position : 4524679
  • Gene Strand (Orientation) : -
  • Protein Name : Vi polysaccharide biosynthesis protein
  • Protein pI : 9.8
  • Protein Weight : 21113.2
  • Protein Length : 179
  • Protein Note : Similar to Salmonella typhi Vi polysaccharide biosynthesis protein TviA SW:TVIA_SALTI (Q04971) (179 aa) fasta scores: E(): 0, 100.0% id in 179 aa and to Salmonella paratyphi C VipR protein TR:BAA24231 (EMBL: AB006131) (179 aa) fasta scores: E(): 0, 100.0% id in 179 aa
  • DNA Sequence : Show Sequence
    >GeneID|1250863 [Salmonella enterica subsp. enterica serovar Typhi str. CT18 ] 4524140..4524679
    ttacagtaaagtaactgaatccggcaataacagatagcgcggctcacgtactccaaattttactgccaat
    tcttttctccagcgatacatagtcgatgatgagttcataaatctatcctgaagctcttccataccacttt
    ccgaaaggaaatatttcatctttactatatccttacggttaattctgaaacgataatctctctgctccgt
    caagatcttttcaccactttggtgttgactgacatttctgataatatcctggactgatgttgccgcatcg
    aaaacagcaataacatttttgcactctgtcaaccaaaaacgtgcaaggggcattagtcgcgaacttgaaa
    tgataacgattattttattaatatcactcattcgcgtgaaaaaattcagggcatttaacaggctgtagcg
    atttaggttcacaaacaaataatttctgtcatttgttatcagtgacacttcttcaataatgccagcagct
    ccaaccccgaaatagatatcattcggaggccagaaatgatgaaacctcat
  • Protein Sequence : Show Sequence
    >gi|16763124|ref|NP_458741.1| Vi polysaccharide biosynthesis protein [Salmonella enterica subsp. enterica serovar Typhi str. CT18 ]
    MRFHHFWPPNDIYFGVGAAGIIEEVSLITNDRNYLFVNLNRYSLLNALNFFTRMSDINKIIVIISSSRLM
    PLARFWLTECKNVIAVFDAATSVQDIIRNVSQHQSGEKILTEQRDYRFRINRKDIVKMKYFLSESGMEEL
    QDRFMNSSSTMYRWRKELAVKFGVREPRYLLLPDSVTLL
  • Related Vaccine(s): CVD 909
56. TviB
  • Gene Name : TviB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. Ty2
  • VO ID : VO_0011269
  • NCBI Gene ID : 1067316
  • NCBI Protein GI : 29144603
  • Locus Tag : t4352
  • Genbank Accession : AE014613
  • Protein Accession : NP_807945
  • Taxonomy ID : 209261
  • Gene Starting Position : 4505426
  • Gene Ending Position : 4506703
  • Gene Strand (Orientation) : -
  • Protein Name : VI polysaccharide biosynthesis protein, UDP-glucose/GDP-mannose dehydrogenase
  • Protein pI : 5.79
  • Protein Weight : 43904.75
  • Protein Length : 425
  • Protein Note : corresponds to STY4661 from Accession AL513382: Salmonella typhi CT18
  • DNA Sequence : Show Sequence
    >gi|29140543:4505426-4506703 Salmonella enterica subsp. enterica serovar Typhi str. Ty2, complete genome
    ATTACAATCTCACATCTGACTGCTCAGCCGGAAGAACATACTTCAAATCATAAAGTACATGTTTATCTTT
    TCCGAAGCCGCGAATATCCTCACTTCCCATCTGTTTAAATTGCTGATGTCCTACTGCAACAATGATCGCA
    TCGTAGTGGCTTGATTTGACTTCCGATACCGGGATAATGCCATACTCTCGTCTTACCTCTTCGGCATCCA
    CCCATGGATCAAAAATATCCACTTTACAACTATATTTACCGAGTTCCTTTACCACATCAATAATGCGTGT
    ATTTCTGATGTCCGGACAGTTTTCTTTAAAGGTAAAGCCGAGAATCAGCACGCTGGAACCCTCAACGTTA
    ATTCCTTTTTTGATCATTGCTTTGATCAACTGCTCGGAGACATAGTTGCCCATGTTGTCGTTCAGGCGGC
    GTCCTGCAAGTATGATTTCTGGATAATAGCCAATGCCCTGAGATTTATGTGTCAGATAATAGGGATCTAC
    GCCAATACAGTGACCACCGACCAGTCCCGGACGGAATGGCAGGAAATTCCATTTGCTGCCAGCGGCACGT
    AGCACGGCTTCAGTATCGATATTTAAACGATTAAAAATAATCGCCAGCTCATTGACCAAGGCAATATTCA
    GATCGCGTTGCGTATTTTCAATCACCTTCGCTGCCTCAGCAACTTTGATGCTCTCTGCTTTATATGTACC
    TGCGCTGATGATCTGCTGATATACTTCATCGATAAGTTCGGCGATCTGTGCGGTTGAACCGGAGGTGATT
    TTCTTGATGTTGGTTAAACGGTGCTTTTTATCACCGGGATTGATCCTTTCCGGGCTATAACCGACATAGA
    AATCCTGGTTGAAAGTCATTCCGGACATACGAGCAAGGATCGGCACGCATTCTTCTTCGGTACATCCCGG
    ATATACCGTAGATTCATATACCACAATATCTCCCCGATTCAGCACTGTACCAACGGTTTCACTCGCCTTG
    ATTAGTGGGGTGAGGTCTGGTTGCTTGTAGGTATTTATCGGCGTCGGGACGGTGATGATGTAAAAATTAC
    ATTCTTTGATCTTCTCAATCTCGGAAGTAAATTTCAGATAACGAGCCTCACGTAATTCTTCTTCAGTGGT
    TTCCAGATTGACATCCACCCCATTCTTTAATTCAAGAATACGCTTTTTATTAACGTCGAAGCCAACAACC
    TGACGAGATTTGCCAAATTCAACTGCCAGAGGAAGCCCAACATATCCCAGCCCAATAATCGCGATTTTTA
    CCTCGTCTATACCGAACA
  • Protein Sequence : Show Sequence
    >gi|29144603|ref|NP_807945.1| VI polysaccharide biosynthesis protein, UDP-glucose/GDP-mannose dehydrogenase [Salmonella enterica subsp. enterica serovar Typhi str. Ty2]
    MFGIDEVKIAIIGLGYVGLPLAVEFGKSRQVVGFDVNKKRILELKNGVDVNLETTEEELREARYLKFTSE
    IEKIKECNFYIITVPTPINTYKQPDLTPLIKASETVGTVLNRGDIVVYESTVYPGCTEEECVPILARMSG
    MTFNQDFYVGYSPERINPGDKKHRLTNIKKITSGSTAQIAELIDEVYQQIISAGTYKAESIKVAEAAKVI
    ENTQRDLNIALVNELAIIFNRLNIDTEAVLRAAGSKWNFLPFRPGLVGGHCIGVDPYYLTHKSQGIGYYP
    EIILAGRRLNDNMGNYVSEQLIKAMIKKGINVEGSSVLILGFTFKENCPDIRNTRIIDVVKELGKYSCKV
    DIFDPWVDAEEVRREYGIIPVSEVKSSHYDAIIVAVGHQQFKQMGSEDIRGFGKDKHVLYDLKYVLPAEQ
    SDVRL
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : The viaB (tviB) gene coding for the Vi antigen of Salmonella typhi Ty2 was subcloned into expression vector pYA248. The recombinant plasmid was termed SMM202 and transformed into Salmonella typhimurium χ4072, an attenuated ΔcyaΔcrp mutant. Immunization of mice with Vi4072 afforded complete protection against fatal infection with virulent S. typhi Ty2 (Cao et al., 1992).
  • Related Vaccine(s): S. typimurium Vi4072
57. Ty2
  • Gene Name : Ty2
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi Ty2
  • NCBI Gene ID : 1070398
  • NCBI Protein GI : 29144781
  • Locus Tag : t4545
  • Genbank Accession : NC_004631.1
  • Protein Accession : NP_808123.1
  • Taxonomy ID : 28901
  • Gene Starting Position : 4695660
  • Gene Ending Position : 4698959
  • Gene Strand (Orientation) : -
  • Protein Name : hypothetical protein
  • Protein pI : 7.02
  • Protein Weight : 125222.8
  • Protein Length : 1099
  • Protein Note : corresponds to STY4851 from Accession AL513382: Salmonella typhi CT18
  • DNA Sequence : Show Sequence
    >GeneID|1070398 [Salmonella enterica subsp. enterica serovar Typhi Ty2 ] 4695660..4698959
    taacattacgcaatagacagtattggaaaatggcgttaaaccatcgtgagtcaaaatctctaacctgtct
    caagaaaaatacggcgccgggcttattctcaaaaacactttcaaatgttgttttaccgctggcaacagca
    gcagcaaatacggggagcaaggtgacggaatagcggtagctggcatcaggggaaatatcgataacaggat
    tttgttgcgacatataccaacgtaaaaggcctgggccaccaaattctggccagcgagattcaccatgttc
    gcgtagaagttcctgataccactcaaggataatacctttcatcgtcaactcaggaaaaaccggtggtaat
    ttcccttgactcaaccataattgcacctcactggcataagtcgggaatgttgtgcccagctgttgataca
    ttctgtacagcaaccttatctgcatttcctccggtgcacctttatgagtcaaaaaagcacgaaaacgtgt
    ggcggaacgtttaacttctgtaatggataaaaactcccagaaaacgggaaactcactttcaatacggctg
    agataatcaatggacatttcaaatttgaacagcgacatcgccaacgcttgtggatgctgaacaagtgccc
    gccatacttcaaaggtagccaaaggtaaatacccgaactgatcgtataaattgcgtaagaactgccagcc
    cgagtgtgccgggtcgctcgccatttgttccagaactaacataatggtgttgacatctgaacgaggatta
    aaaagctgcgttgctttttgtaaagactgtattgctgtcgcgtcgctttgaattaccggctcacctgcaa
    taaagcaaggtcgaaatgaggcttcttcacctggtttcggcagcaccagccatgggccattcttttgtat
    tatcgaacttagttcaaattcaccaactggtacaccttcgctcattcttgacgtcagcagtacaactttt
    ctctccggttcgctgagtaacataatgacgggcgaagggatctggcctgtacgattacttattgagtttg
    cgagaagaatctgcctgccacgatcatagtccagactatatttataacgtcggatctgccaggtaaaaga
    actaccacctccttttatgcgcatatcaactgtttgatcaattccttcctccagggagagtaaattatca
    atatgttcccttaagctatataaagtaagctctaccggacattctcccgcactgtagtgccactcatacc
    aggcctgcattccactacgcgaacgtaaacgtagttcaagctggtatcgcgtaggctcaccattttttcc
    gaaaagatatgctctggcacccagaagatcgttaatcgttaaatttttcgggagaggtttctcatcttta
    tcgaacgcaagacagcctcgtgcaggaaatggtaaccaaataacaatcgggtttgcagtgaggtttggcg
    ttatttgtaatgaaattgatgcaggaggaatgccctccgccttcatcattatttctgtactatcgggcaa
    ccgcttccgtccaacttcaagtgttttttccttcaagctatacaaacaagggtgctgagtagtaataatg
    acagaaccttcgttcgcctgttgtccattttttatttccagactaaaatcattggggagaatgccaattt
    tcttacgcagtaacgtttcatcattttcgttacgcaccgaaataaactgtgctcccattttttcttgtac
    atcacaattttctataaatgttccgttgaaaaaacgcttgtaatggcgcgttgatagattctcagaatgt
    tgagtaattccaggaacaccgaggaagagttcatccggataacttgtccaatttaatcgcttcccctgga
    aagagaatccagtatggataatctgatctcttcctatctttatccggtacgtttcatcgcctttaatgat
    gatatcctggcgacctttgatggtcagagcatggcatcccaaagccgaaaagccagagtcacaatcctca
    tgttctgaagccagacagccatcctttggcagcacaataagtacatggctgccacgtacgctgcaagagg
    cttgtccctgtaacagccattcatttttatcagaaacaaaaaccaatggaacatcgccaaccgcaacttc
    actgccttctaaaagattggagccgaaaaacatacccccagctctcgccacaataaacaaactgaccgtg
    ggatttcttcggtaaaatttaatctcgcgcttacgaacttttatttttgcctgggagttacttagcgttg
    catatgcaggtcccagacttgcgatttcgttaccatcttcatagatagcaagctcgaagcgcgttgttga
    aggctcaatatcaattgaaaaactcagttcttcaggcagagaaatgagtgcctggagggcttcaggatga
    ttttctgaccataaaaactgacagagagtggttttattcttttgaaggcggggttttgattcagtagaag
    ctgttcgtaaaagtccattaaggaaactggtacccgtatcatcatcgagagggacagggaaactgtcacg
    ccacttaggatgaacacgttcaagttcttttacgggttcggtatgattacttaaatcataaatttgaaca
    agagatactaactgttcagccatccgactaataagctcaactgaagtatcctctttgaaaacgactggca
    gggatgatttttctaccgctgcatggactaacgcgaacgtggaaatgttagaagatttagcctgatcgta
    ttgatttagaatgagagaaaacatcgactgaaaacggctattagattcctttagcaacctgaaaggtaga
    ccgccttcactgaataaggagcctaagaaattccgcctttctgtgtcataaaaccgcactggacgccccc
    agtaaccatccaggcctttagggataattttccccattttcgaggaagatgctgagatacctatcgtctt
    gtaaattggctcccatgcccatccatactctctttcataatctcttcgataccactctgagcagaacaat
    gtgaaacaggctgcgtaccctttatcacttttataaccatcgggtacccctatagctcgcagcagccgtt
    tcaattcatcatattcttcactcgttgcgtggtattcatataacggacgatggtctggttgcttcaatcc
    tctgcgggtgaggaatttagctagccatggggtacactttagaatggatgcgtattcggactgactcaaa
    gcaagctcct
  • Protein Sequence : Show Sequence
    >gi|29144781|ref|NP_808123.1| hypothetical protein [Salmonella enterica subsp. enterica serovar Typhi Ty2 ]
    MSQSEYASILKCTPWLAKFLTRRGLKQPDHRPLYEYHATSEEYDELKRLLRAIGVPDGYKSDKGYAACFT
    LFCSEWYRRDYEREYGWAWEPIYKTIGISASSSKMGKIIPKGLDGYWGRPVRFYDTERRNFLGSLFSEGG
    LPFRLLKESNSRFQSMFSLILNQYDQAKSSNISTFALVHAAVEKSSLPVVFKEDTSVELISRMAEQLVSL
    VQIYDLSNHTEPVKELERVHPKWRDSFPVPLDDDTGTSFLNGLLRTASTESKPRLQKNKTTLCQFLWSEN
    HPEALQALISLPEELSFSIDIEPSTTRFELAIYEDGNEIASLGPAYATLSNSQAKIKVRKREIKFYRRNP
    TVSLFIVARAGGMFFGSNLLEGSEVAVGDVPLVFVSDKNEWLLQGQASCSVRGSHVLIVLPKDGCLASEH
    EDCDSGFSALGCHALTIKGRQDIIIKGDETYRIKIGRDQIIHTGFSFQGKRLNWTSYPDELFLGVPGITQ
    HSENLSTRHYKRFFNGTFIENCDVQEKMGAQFISVRNENDETLLRKKIGILPNDFSLEIKNGQQANEGSV
    IITTQHPCLYSLKEKTLEVGRKRLPDSTEIMMKAEGIPPASISLQITPNLTANPIVIWLPFPARGCLAFD
    KDEKPLPKNLTINDLLGARAYLFGKNGEPTRYQLELRLRSRSGMQAWYEWHYSAGECPVELTLYSLREHI
    DNLLSLEEGIDQTVDMRIKGGGSSFTWQIRRYKYSLDYDRGRQILLANSISNRTGQIPSPVIMLLSEPER
    KVVLLTSRMSEGVPVGEFELSSIIQKNGPWLVLPKPGEEASFRPCFIAGEPVIQSDATAIQSLQKATQLF
    NPRSDVNTIMLVLEQMASDPAHSGWQFLRNLYDQFGYLPLATFEVWRALVQHPQALAMSLFKFEMSIDYL
    SRIESEFPVFWEFLSITEVKRSATRFRAFLTHKGAPEEMQIRLLYRMYQQLGTTFPTYASEVQLWLSQGK
    LPPVFPELTMKGIILEWYQELLREHGESRWPEFGGPGLLRWYMSQQNPVIDISPDASYRYSVTLLPVFAA
    AVASGKTTFESVFENKPGAVFFLRQVRDFDSRWFNAIFQYCLLRNVTEK
58. wecA
  • Gene Name : wecA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • NCBI Protein GI : 20140863
  • Other Database IDs : CDD:185074
    CDD:133463
  • Taxonomy ID : 90371
  • Gene Strand (Orientation) : ?
  • Protein Name : Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase
  • Protein Length : 367
  • Protein Note : UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase; Undecaprenyl-phosphate GlcNAc-1-phosphate transferase
  • Protein Sequence : Show Sequence
    >gi|20140863|sp|Q9L6R7.1|WECA_SALTY RecName: Full=Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase; AltName: Full=UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase; AltName: Full=Undecaprenyl-phosphate GlcNAc-1-phosphate transferase
    MKLLTALSELISIFLFTTIFIFLARKVAIKIGLVDKPNFRKRHQGVIPLVGGISVFAGICFMFGLSDYYI
    PHLSLYLICAGVLVFVGAMDDRFDISVKIRAVVQAVIAVVMMVIAKLHLGSLGYIFGPWELVLGPFGYFL
    TLFAVWAAINAFNMVDGIDGLLGGLSSVSFAAMGLILWFDGQTSLAMWCFAMIAAILPYIMLNLGILGRR
    YKVFMGDAGSTLIGFTVIWLLLETTQGKTHSISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPDRQHI
    HHLVMRAGFTSRQAFVLITLAAAILAGVGVTAEYSHFVPEWVMLVLFLLAFFLYGYCIKRAWKVARFIKR
    VKRRLRRQRENRPNLTK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A wecA mutant (DSM645) is attenuated in C57BL/6J mice through i.p. and oral immunization. This mutant also protects against challenge with S. typhimurium strains TML and SL1344 30 days after immunization (Gilbreath et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium wecA mutant vaccine
59. znuA
  • Gene Name : znuA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1253412
  • NCBI Protein GI : 161353595
  • Locus Tag : STM1891
  • Genbank Accession : AE006468
  • Protein Accession : NP_460848
  • 3D structure: PDB ID : 2XY4
  • Taxonomy ID : 700720
  • Gene Starting Position : 1986414
  • Gene Ending Position : 1987358
  • Gene Strand (Orientation) : -
  • Protein Name : high-affinity zinc transporter periplasmic component
  • Protein pI : 5.71
  • Protein Weight : 31911.63
  • Protein Length : 314
  • Protein Note : Also known as yebLinvolved in transport of zinc(II) with ZnuA and C
  • DNA Sequence : Show Sequence
    >gi|16763390:1986414-1987358 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    GTTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGGCTTAAAAACGCTGAATAGCTTGTTTT
    ACCCAGTTTAATGTTCGTCCCGAGGGGGTCCAGTGTTCCCATTCGAACGGATGTCCCTCTCGCCACGGCT
    TCCACGACCGCTGGCCTGAATTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTCAACCAACTGTGTTC
    TTATTTCATGTAAACGCTGCGCGCCAGGTTGTATCTCAGGGTTCACGGTAAAGTGACCGAGCGGGGTGAG
    TCCGTAGTGTTTTTCGTAGTAACCGTAGGCGTCATGAAAAACGAAATACCCTTTCCCCTTGAGCGGCGCG
    AGCTCGTTACCGACCTGTTTATCGGTTGCGGCTAATTGTGCCTCAAAATCCTTCAGGTTGGCGTCGAGTT
    TGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTTCATGGATTGCAACCGCTGTAGCCCGCGCTATCTC
    TGGGGAGAGCCAAAGATGCATGTTATATTCGCCGTGATGGTGATGTACGTCACCTTTTTCCTCATCGGCG
    CCCGTATGCGCATGTTCATCCTCATCATCATCCGCGCCCTTCATGAGTAACGGTTTTACATCGGCAAGTT
    GCGCAATGGTAACCTGCTTATTATCAGGGATATTCCTGACCGACTTCTCCATAAAGGCTTCCATTTCCGG
    GCCAACCCAGACGACTAAGTCCGCGCCCTGTAAGCGTTTTACGTCTGATGGACGCAATGAATAATCATGC
    TCGGAAGCCCCATCCGGAAGTAATACTTGCGTATCCGTAACGCCATCAGCAATGGCGGAAGCGATGAACC
    CAAGCGGTTTAAGCGAAGCGACAACGGCGGCGTCGGCAGCCTGGGTTGCACTCCCCCAAAGCGCGGCGGA
    TAATGCTGCGAAAAGAAGCGTATTTTTCTGTAACA
  • Protein Sequence : Show Sequence
    >gi|161353595|ref|NP_460848.2| high-affinity zinc transporter periplasmic component [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MLQKNTLLFAALSAALWGSATQAADAAVVASLKPLGFIASAIADGVTDTQVLLPDGASEHDYSLRPSDVK
    RLQGADLVVWVGPEMEAFMEKSVRNIPDNKQVTIAQLADVKPLLMKGADDDEDEHAHTGADEEKGDVHHH
    HGEYNMHLWLSPEIARATAVAIHEKLVELMPQSRAKLDANLKDFEAQLAATDKQVGNELAPLKGKGYFVF
    HDAYGYYEKHYGLTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVEAVARGTSVRMG
    TLDPLGTNIKLGKTSYSAFLSQLANQYASCLKGD
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A znuABC mutant is attenuated in mice and induced significant protection against a challenge infection in the large intestine with wild type Salmonella typhimurium (Pesciaroli et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium znuABC mutant vaccine
60. znuB
  • Gene Name : znuB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1253414
  • NCBI Protein GI : 16765235
  • Locus Tag : STM1893
  • Genbank Accession : AE006468
  • Protein Accession : NP_460850
  • Taxonomy ID : 700720
  • Gene Starting Position : 1988189
  • Gene Ending Position : 1988974
  • Gene Strand (Orientation) : +
  • Protein Name : high-affinity zinc transporter membrane component
  • Protein pI : 8.72
  • Protein Weight : 24998.23
  • Protein Length : 261
  • Protein Note : Also known as yebIinvolved in transport of zinc(II) with ZnuA and C
  • DNA Sequence : Show Sequence
    >gi|16763390:1988189-1988974 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    CATGATTGAATTATTACTGCCCGGCTGGCTGGCCGGGATGATGCTGGCCTGCGCCGCTGGTCCTTTGGGC
    TCATTTGTGGTCTGGCGTCGGATGTCCTATTTTGGCGATACGCTGGCTCACGCGTCGCTTCTGGGCGTGG
    CGTTTGGCCTGTTGCTGGACGTTAACCCCTTTTATGCGGTAATCGCCGTTACGCTGCTGCTGGCCGCCGG
    TCTGGTATGGCTGGAGAAACGCCCTCACCTCGCGATTGATACCTTACTGGGCATTATGGCGCACAGCGCC
    TTATCTTTAGGACTGGTCGTGGTCAGCCTGATGTCCAACGTCCGCGTTGATTTAATGGCTTATCTGTTTG
    GCGATTTGCTGGCCGTCACGCCGGAAGATCTTATCTCGATCGCTATTGGGGTCGTTATTGTGCTGGCCAT
    TCTCTTCTGGCAGTGGCGCAACTTGCTGTCAATGACTATCAGTCCGGATCTTGCCTTTGTCGATGGCGTG
    AAGTTACAGCGCGTGAAGCTGCTATTGATGCTGGTAACGGCGTTAACTATTGGCGTGGCGATGAAGTTTG
    TCGGAGCGCTGATCATTACGTCGCTGCTGATTATCCCCGCCGCAACCGCGCGTCGCTTTGCCCGTACGCC
    GGAGCAGATGGCGGGCGTCGCCGTTGGTGTAGGTATGATAGCGGTTACAGGTGGACTGACCTTCTCAGCC
    TTTTATGATACCCCGGCAGGGCCATCCGTAGTGCTGTGTGCCGCGCTGCTGTTTATCTTCAGTATGATGA
    AAAAGCAGGCAAGCTA
  • Protein Sequence : Show Sequence
    >gi|16765235|ref|NP_460850.1| high-affinity zinc transporter membrane component [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MIELLLPGWLAGMMLACAAGPLGSFVVWRRMSYFGDTLAHASLLGVAFGLLLDVNPFYAVIAVTLLLAAG
    LVWLEKRPHLAIDTLLGIMAHSALSLGLVVVSLMSNVRVDLMAYLFGDLLAVTPEDLISIAIGVVIVLAI
    LFWQWRNLLSMTISPDLAFVDGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTP
    EQMAGVAVGVGMIAVTGGLTFSAFYDTPAGPSVVLCAALLFIFSMMKKQAS
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A znuABC mutant is attenuated in mice and induced significant protection against a challenge infection in the large intestine with wild type Salmonella typhimurium (Pesciaroli et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium znuABC mutant vaccine
61. znuC
  • Gene Name : znuC
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1253413
  • NCBI Protein GI : 39546331
  • Locus Tag : STM1892.S
  • Genbank Accession : AE006468
  • Protein Accession : NP_460849
  • Taxonomy ID : 700720
  • Gene Starting Position : 1987437
  • Gene Ending Position : 1988192
  • Gene Strand (Orientation) : +
  • Protein Name : high-affinity zinc transporter ATPase
  • Protein pI : 8.96
  • Protein Weight : 25186.17
  • Protein Length : 251
  • Protein Note : Also known as yebMinvolved in transport of zinc(II) with ZnuA and C
  • DNA Sequence : Show Sequence
    >gi|16763390:1987437-1988192 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
    CATGACAAGTTTAGTTTCACTGGAAAACGTCTCGGTCTCATTTGGTCAACGCCGCGTCCTCTCTGACGTG
    TCGCTTGAATTGAGCCCCGGAAAAATTTTAACGCTTCTCGGCCCTAACGGCGCGGGGAAGTCAACGCTTG
    TACGCGTGGTTTTAGGACTGGTAGCCCCTGATGAAGGGGTGATCAAGCACAACGGGCAACTCCGTATCGG
    CTATGTCCCGCAAAAGCTCTATCTCGATACCACGCTTCCGCTGACGGTAAACCGATTTTTACGTTTACGT
    CCCGGCACGCAAAAAACGGATATTCTCCCGGCGCTTAAACGCGTTCAGGCCGGACATTTGATTGACGCGC
    CCATGCAAAAACTCTCCGGCGGTGAGACACAGCGCGTACTGCTGGCGCGTGCTCTGCTTAACAGGCCTCA
    GTTGCTGGTACTTGATGAGCCGACGCAAGGCGTGGATGTCAACGGCCAGGTCGCGTTATACGACCTTATC
    GATCAGCTGCGTCGCGAACTCGATTGCGCCGTGCTGATGGTGTCACATGACCTACATCTGGTCATGGCAA
    AGACGGATGAAGTGTTATGCCTGAACCATCATATTTGCTGTTCCGGCGCGCCGGAAGTGGTGTCTATGCA
    TCCGGAATTTATCTCTATGTTTGGCCCGCGAGGCGCGGAGCAATTAGGGATTTATCGCCATCATCATAAT
    CATCGCCACGATTTACAGGGTCGTATTGTACTGCGCCGGGGAAATGGTCACTCATG
  • Protein Sequence : Show Sequence
    >gi|39546331|ref|NP_460849.2| high-affinity zinc transporter ATPase [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
    MTSLVSLENVSVSFGQRRVLSDVSLELSPGKILTLLGPNGAGKSTLVRVVLGLVAPDEGVIKHNGQLRIG
    YVPQKLYLDTTLPLTVNRFLRLRPGTQKTDILPALKRVQAGHLIDAPMQKLSGGETQRVLLARALLNRPQ
    LLVLDEPTQGVDVNGQVALYDLIDQLRRELDCAVLMVSHDLHLVMAKTDEVLCLNHHICCSGAPEVVSMH
    PEFISMFGPRGAEQLGIYRHHHNHRHDLQGRIVLRRGNGHS
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A znuABC mutant is attenuated in mice and induced significant protection against a challenge infection in the large intestine with wild type Salmonella typhimurium (Pesciaroli et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium znuABC mutant vaccine
1. Ifng (Interferon gamma)
  • Gene Name : Ifng (Interferon gamma)
  • Sequence Strain (Species/Organism) : Mouse
  • NCBI Gene ID : 15978
  • NCBI Protein GI : 33468859
  • Genbank Accession : NM_008337
  • Protein Accession : NP_032363.1
  • Other Database IDs : MGI:107656; UniProt: P01580
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • DNA Sequence : Show Sequence
    >gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
    ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
    GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
    GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
    AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
    AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
    GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
    AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
    GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
    AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
    AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
    AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
    TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
    TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
    ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
    GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
    AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
    TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
    AGTATACCTTTGCAACC
  • Protein Sequence : Show Sequence
    >gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
    MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
    ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
    LLPESSLRKRKRSRC
  • Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
  • Additional Molecule Role : Vaximmutor
  • Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
  • Related Vaccine(s): Salmonella Typhimurium hfq mutant vaccine , Salmonella Typhimurium ruvB mutant vaccine
2. IgA
3. IgA
  • Gene Name : IgA
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Protein GI : 2632187
  • Other Database IDs : CDD:209398
    CDD:197704
  • Taxonomy ID : 9606
  • Gene Strand (Orientation) : ?
  • Protein Name : IgA
  • Protein Length : 131
  • Protein Note : Immunoglobulin domain; cl11960
  • Protein Sequence : Show Sequence
    >gi|2632187|emb|CAA10818.1| IgA [Homo sapiens]
    LQQWGAGLLKPSETLSLTCAVYGGSFSGYYWSWIRQPPGKGLEWIGEINHSGSTNYNPSLKSRVTISVDT
    SKNQFSLKLSSVTAADTAVYYCASRVGYCSSTSCYTFDYWGQGTLVTVSSASPTSPKVFPL
  • Molecule Role : Vaximmutor
  • Additional Molecule Role : Vaximmutor
  • Related Vaccine(s): Aro-deleted S. Typhi , CVD 908-htrA
4. IgA Fc receptor
  • Gene Name : IgA Fc receptor
  • Sequence Strain (Species/Organism) : Gallus gallus
  • NCBI Gene ID : 771853
  • Taxonomy ID : 9031
  • Chromosome No : 26
  • Gene Starting Position : 2406653
  • Gene Ending Position : 2408482
  • Gene Strand (Orientation) : -
  • Protein Name : Fc receptor, IgA, IgM, high affinity
  • DNA Sequence : Show Sequence
    >gi|118135660:2406653-2408482 Gallus gallus chromosome 26, reference assembly (based on Gallus_gallus-2.1)
    ATTAATACTTGGCTAACATTCCTACTTCAGCTATCAGGGACAGAAATGATGAGGGTGAAGAGTGAATTGC
    ACCTGTGTCTCTGCATGCTGAGGTTCCTAGAACTGCAAGATAACCTTCAGAGCAAAGCACATCTCCATGC
    TGCATGTGTTGTGCTCAGGATGCCATGTGCTGGTGTTTTCTATCATAAATTGTCCATGATATTTTCAAAC
    AAACTTGAGCAGAAAGCTCAAATGTTTCTTGTAAAGCCACTACATTTATGCATGGGACAAAGGAGAGAAT
    TTGAAGGGGGTATCCATGAAGGGATAGGGGGAAGAGTGATTAACAGAAGGAAAAGGGTTGATTTGTGTTT
    CCAGCCAGCCATCGCCTACCCGTCTCGATGCGCAGTTTCATGGAGGTCGTGTGCTCCCTGTGCCTGTTCC
    TGGCCACACACCAGTACCACCCCGCGTCCTCCTCCCGCAGGTGGCTCATCACCACGGTGTAGGTCCCACT
    GTCCTGCTCACTGTTGCTGATCCATATGCGCCCTTTGTAGGACTCGTGCACAAACCCGTCTGTGTCTACC
    AGCAGGGAGCAGCTGGCCTCCTGCCACCTGCACAGGTACTTGGTGGTGTAGCTGCCCACGGGGTTGTGGT
    GGCACCTAAGGGACAGTGAGCCCCCCAGCCTGCCATGCAGAAACTTCAACCTCCGGGGCTGGGCAGGGAC
    TGCAGGTAGAAGAAATCATGGTTATGTTTGATGTAGCTGCAATGAGCCAATGGGGCACAGCAGCCCCCAA
    AACCCCCTCCTACCTGTGGTCACCTGCAGGGCCACCAGCTGCCAGCTGTCCCAGTCAGCTGGGCTGCCCA
    TCCCACACTTGTACAGCCCTGCATCGTCCAACCTTAAATCATTTATCAGCACTTTGAATGCTCCAGAGCT
    CTCCTGGGGGGTAATACAGATCCGTCCCTCGTAGCTCTTCCCCACATAGCCATCGGTGTCAGCAATGAGG
    ATGCACCTGCTTCTCCCCACTCTGCACCATAGCCTCCTGGTGCCACCCCAGCTGTCCCCGGGTGGGCAGG
    GGATGGTGATGGAGCCACGGAGCTCCCCGAGGTGCAGCTCAATTGGCCTCTGAGTGCCCACATCTGCAAA
    GACAGAGCAGGTGAGTGCCACCACTGTGCCTGGTTGGGTGTCACCTCCTTTTGCATTGGTGCATTGGTCA
    CATCAGTGGGTTTTATGCCCACGACTGCTTAAAGCAAAGGGTAGCAAAGGGGACCTGACACCCTCCCTGT
    CCCATCACTGTACCTGCTGACACTGTCAGGTTCAGGCTGACGTAGAGGTCCCTGTTGGTGGGGCCAATGC
    CACAGCGGTAGGTCCCTGTGTCACTTGTCTTCAGCTGTGTCATTGTAACCATGAAAGTGCCATGCTGGGG
    GACGTCCTCCAGGGCCACTCGGCCCATGTGGTCTTTTGAGATGAAGTTGGTGGTGGAGATGATGGTGTAG
    CAAATCCCATCTCTGTCCCTTTTACACCAAAACTTTCTGTCGTATTTGTTGGCAGGCGTGATGGAGTAGA
    AGCACTGGTGGGTGATGGACCCTCCAACTGCGCCTGTCAAAAACCTGGGCCCATACAAGGTGCTTGAGGC
    TGCCTCTTTGGGGAAGGAGGGAAGAAGCACATTAATCAGAGACGTGGACACCCCTGTGGATGGGGAATCT
    TCCCCAACCCAAATGGGGTGCCACAGGCACAGGTCCACCAGCCCTGGCTGTCCTGCCCCATAGTGATGGG
    GAGCAGCAAACCTTTGGTGCCTGCAGAGTTTTCTGGTGACTCACCTTGCAGCAAAGACACAAAGAGGAGG
    AGAACCTCCA
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Gallinarum relA/spoT mutant vaccine
5. IgG
  • Gene Name : IgG
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16059
  • Genbank Accession : AF010213
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 881411
  • Gene Ending Position : 914690
  • Gene Strand (Orientation) : +
  • Protein Name : immunoglobulin heavy chain (V7183 family)
  • Protein Note : Also known as IgG; IgH; VI24H; VH7183; B9-scFv; IgVH1(VSG)
  • DNA Sequence : Show Sequence
    >gi|94393741:881411-914690 Mus musculus strain 129/SvJ chromosome 12 unlocalized genomic contig, MGSCv37 alternate locus group 129/SvJ
    CATGGACTTTGGGCTCAGCTGTGTTTTCCTTGTCCTCATTTTAAGAGGTAATTTGTAGAAATAAGATCCT
    GCCAGTATTGTGTACAGGAGAAATAGAAAAATTTTTCTTTCCTCTATTTTGTTTTGTTTTGTTAGTGACA
    GTTTACAAATAAGCATTCTCTGTTGTGAGGTGCCCAGTGTGAGGTGAAGATGGTGGAGTCTGTGGGAGGC
    TTAGTGCAGCCTGTAGGGTCATTGAAACCCTCCTGTGCAGCCTCTGGATTCATTCTCACTGACTACTGAA
    TGACCTGGATCCTTCAGGCTTCAAAGAAAAGGATGGAGAGGGTGACAATAAAATTTTTCCCACTCACACC
    CACTTGCTGGCCCTGGCGTTCCCCTGTAATGAGGCACATAAAGTTTGCAAAACCAAGGGGCCTCTCTTCT
    CAATGATGGCTGACTAGGACATCTTCTGATACATATGCAGCTAGAGACATGAGCTCCCGGGATACTGGTT
    ACTTCATATTGTTGTTCCCCCACAACAGTTGCAGACCCTTTTATTTCTGTGGGTACTTTCACTAGCTCCT
    CCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTAGTTGCTAGGCCCCGG
    CATAGCCTCACAAGAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTTGCTGGTGTATGCAATGGTGTC
    AGAGTTTGGAGTCTGATTATGGGATGGATCCCCAGGTATGGCAAACTCTAGATGGTCCATCCTTTCCCAA
    CAATAATTTTTAATGGTGGAGATAGCACCTACTATCCAGACACAATGAAGGGCCAATTCACTATCTCTAG
    AGATGATGCCAAAAACACACTTTACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTATACA
    TATATTCCTAAGCTTGAGTAAGTGGACTTAAACTGATTCCATCACAACTTGCATGAGATGGATATTCCCC
    AGTGTTAAGACCTGTCACCATCACTGTCAATCAGAAGACAAAGTTTATGCACAACAAAACAAAAAAACCA
    AAAGCAGAGGCCTCCAATTACAAGTAATAGACCCAGACCCACAGTCTCTGAAAACTGACTGTACAGTTGG
    ATCCAGTCTTTTACTTCTTTTCCCTGGATTTTATATTACTGAGGAAATGAGGAAAGCTCTACAATATCTG
    TTCTCCATAGTGCTCAACACCTCCAAGCACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTTCTT
    GTCTTGTAGACTACTTCAACCTATTTTGTACTCCAGTTAATGAAACTCAAGTCTAGCAGCCTGTCACTGT
    TTATTCTAAAGTATTATGAACAGGTGACCTCCCATCCTTCCCCAACGCAATAATCATATTTAGGAATTTG
    AGGTTTTATGAGATATGATCTCAGGGTAGAGAGAGAAAGCAAACTACATAGAAATATAGACTGACATAAA
    TCAAGACTTGCATAAGCTAGTCCCCAAGTTCCATGTCCCTAAGTGGCAAGGACTATCTTCTGAGCCTAAT
    GAGATGAGATCCAAATCAAACCTCCTGGGTCTTTTTAGATAAACATGTGAGATCAATAGACTAAATGCTT
    TGGCTGGGCTTCCTTGCAATCCAATTCCCAAACAAAAATGGATCTGGCTCCACAGACACCACAAGAATAG
    TCTTAAATAGTTCTTTAAGTAGAATGTCTGATCACTACGAGCCCAATTCCATCCTAAATACTCTTCTGGA
    TTATACATAAATAAAAATTGAACATAGGGCATGGGGCACTGATCTCCCTGTGCTACATGAATGGGGGCTC
    ATTTACTAAATGTTCCCATTTTTCTTTCTAGCGCTGCACAGTGCAAATCCTACAACTTCCTGTTTATCTA
    CAATGTGAACTCCAAACAGTACAAGAAAAAACGTTCCTTATGTTCCTCTCCAGGTTCTCCAACAAGCACA
    GAGCAACCTTCTGTCACCAGGACGGAAAACTGGAAACCTTGCTCACTGCTTCCTTTTATTCCCTCGGGAA
    CCTCCCCCAATGCAAAGCAGCCCTCAGGCAGAGGATAAAAGCTCACACAAAGACGAGAAGCCCCATCCTC
    TTCTCATAGAGCATCCATCAGAGCATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACTTTCCCAAGCT
    GTAAGTGTTTCAGGGTTTCAGAAGAGGGACTAAGACATGTCAGCTAGAAGATGTGTGACTAATGGTGATG
    TTGCTTGTCCCCAGGTGTCCTGTCCCAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCA
    CAGAGCCTGTCCATCACATGCACCGTCTCAGGGTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCC
    AGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGC
    TCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTC
    CAAACTGATGACACAGCCATGTACTACTGTGCCAGAAACACAGTGTGGGAAGTCCAATGTGAGCCTGCAC
    AAATACTTCTCTGCAGGGATGATCACAACCAGCAGGGGGCGCTGATGACCCAAAGGGACTTCCCAGGATC
    TCTTCTGGAATCTAGGGAGTTCTGGCCTGTGTCTATCAGCATGTGTTTCAATGTTAGAGTTGTGAGTTTT
    CCTTCCAGCAACAGAGATATTTTAGAGCCCACTTTTCATGGTCATTCTACTAAATTTGTTCACATAGTGG
    AAAGATTTGTTAAATGATTCTATAGTCTAATAGGGTCAAACAAAAACAAATAATTGAGTTCATATCTACC
    AAAAAAAAAAAAATTCCCTCAAAGTGGACAACTGTGTAGGTGAGGAAAACCAGGGGGATTTGGGAATAGA
    TTATTTCTCTCCCGACTGTGGTGTTAGTCACCTTTCAGCCATTTACTGTATTTAATTCATGTTTAAAGTT
    AATTGTATTCATCTTCCCTAACATGAAGTTTTCAAACACATGTCCACAATTTAATGATGACACAGTTATC
    ATTGTGAATGATAACTCATAAGATCTCTCTCCATATATGAAACACACGATATTCTGTTATTAGCTATAGC
    CAACAAAGTTACATATCATTATTATTACTGAAATAATTCTTCCCATCTAACTGAATAGTTTTCTCACTCG
    ACACTGCCTATGCTGCGATCAGCCTGCTCTAATTTTACCTTTGCTCAGAGCACTGCTTTCTGTTTCATTT
    GATAAGATGGTGCCTGGTTGTCACTGAAAATGTGTCCTCCAGTTTCACAGTGATTAATGTGATTTTCAGT
    ACATTGAGAACAGAGCCACAGCAAAAATGAGATGGAATGTCATCATTTTTACGCCAGGTGCTGCAGAAAT
    AGCTAGGTGGTTAAGAGAGAATATTGAACTTTGAGGGGTTCAGATTAAATTCCTGATACCCACAATGTGC
    ACCTCATAACCACATGTACATATAGAAAACTCAGTATAAATGTGGCCTCCGTGAGCACCACACTACTCCC
    ACAAACACATATACACATAATTAAAAGTAAAGATTTTAGAAAAATGGCTAATCTAATGATAGGAAGTAGT
    CAAAAAGAGAGTTCTTTTGTCATGTACATATGTGTAGCAGACTTAAATGTTAAACATTCAAGAATACATT
    CCTCGATCCACATTAAAATTTTGCAAAGAGTAGCACAGACGGTGGCAATTGCTAAACTTATATCTAGAAA
    CACAATTATGTGTGGTGATATTTAATTACACATTTATACCAGGACATATGACAATATGGAAACCAAACAT
    GTTGTATCCACATGCTCTAAGGAAAACTAAATGGAGTGTGATAAAACCAAAGAAAATGTGAATATGAAAT
    ATTTTTCCAACTCTGCATCTTAAAACGGTTTCTTTCATGTGTCATATCTGCTATGAGGACTTTCTTCTGC
    CCATGTCCAACTCCAGAGCATGCCACAGCAGGAAGACCTACAGGTATTACTTCTCTGCACCCAGGAAAAC
    CACCTCTGTCCTGACCCTGCAGCTCTCAGAAGAGCCCAGACCTTCATTCTCAGGCCCTCATCCAGTAATC
    AGCACTGAATACAGAGCACTCACCATGGACTTTGGGCTCAGCTTGGTTTTCCTTGTCCTTATTTTAAAAG
    GTAATTCATAGAGATAAGATTCTATCTGTTTTGTGTACATGAGAAACAGAAAAATTGTATTGTTTCTCTA
    TTTTGTTTTGTTTTGTTAGTGACAGTTTCTGACTCAAGATTCTCTGTTTGAAGGTACCCAGTGTGAGGTG
    AAGCTGGTGAAGTCTAAGGGGAGGCATAGTGCAGCCTAGAAGGTCCATGATACTCTACTGTGCAGCCTCG
    GATTCACTGTAAGTGACGACTGGTTTGTCCGTGTTTGCCAGGCTCCAAAGAAGGGGCTGCAGTGGGGGAT
    GGGAATAATTTTTCATGGTTGTGGTAGCCCCTCTTATGCAGACACCTTGAAGAAGTGGGTTGGACGTAAC
    ATATTCAGAATCAATATTTAAAGATTCTAATCCTTGAAGATATCACTTTTGACCAAGTATATATGAACCA
    TGTTACTGAGGTTTATGGAGGTTTGAGTATGTTAGGTCCATGGATAGGGAAAATATTAGGGGATTTTAGG
    AGTAACTGAGGCTTGTTGTAGGAAGGACATCACTGTAGGGGTAGGCTCCGTGTTCCTATCTTCAAGCTCT
    ACCCAGTGCAGAATAGAGCCCTCTTCTGCCGGCAAGTGGAAAGAGTTTCTCTTCCTGGCTGCCTTCAATC
    CAAGTTGTAGAATATCAAATCTCCTAACACTATGACTGCGAGCATGCTGACATGCGTCCCACCATAAAAA
    TAGACTGAACCTCTGAAGCTTTAAGCCAGCCTCTAGTAACTGTATTCTTTAATAAGACTGAACTTGGCTA
    TGGTGTCTTTTCACAGTAAAATGGAAACATAGACAAGGTTCTAACTCTCTGTACTAGAGGACCATCTCTA
    TGTCCTTGGTGTTGTATGTAAATTATTGAACAAATACTATAGACAAGTTGTATGGCCAAAAGTCCATCCC
    CTTCAACAATCTACTATCTGGAACCATATAGATTAGATTCCTTTCCTGCACTCATTTCCCTTACTTGCTG
    AGACATTTTGAAGACATGCTCAGAATCCCTGAACTTCCTGCTGAAAAAAAAATACCCCTCCAATTTGAAG
    ACAGTTCTCTTCCAAAATTCATCATAAATACTGATCCACATGTCCAGGCATATCATGTAGTTTTAAAAAA
    CAAACCAATCAATGCTAAAGTGGGTAAGTGCCTACATCATTGGTTTATGTCTGTTCATAACCTGGTATAT
    AGCTAGAGACCAGGACTGCATGTTTCTCCTAGGCCACACCTCTCCCACAAATGCTGGCTCTGCCTCTCCA
    GAATTCCAAGATCATGGATCTCTGGGTCCCCAAGGAACAATAAGTGTGCACTCTTGCCAGCCTGTACACT
    ACTGCACTAGCTTCTCCCCCTGGAGCTTGGTCATTGCTCAATCTTCCCTTTCCAAAGCCTGGCCAAATTC
    CTCCTACCTTTTGTTCTTTCAGTCAGGCCACCTAAGTCATCTTGAATGAAAAACAACACAAAATCTGAAC
    TTAAAATCAACAAATAATACAAGTGCTGCGCACAAAATCAATCATTCTAAACTCATCAACTATTTTATGA
    TGGAAATCTTCCAACATACAAGCTACCACCAGGTCTAAGAATTACTCATCTACATGTTGCTTCCTCTCAC
    TCACAGCCTAACCATAAAAGGGCTGTTTCTTCCTCCCATGTCCCCTCTTTTGCCTTCAGAAGCAGAAGCT
    CCACCTCCTCCTCTTTGCCCAGCAATTGGCTTCTTGTCGTCTTTATTATCATATTAATTACTTAGGGGAA
    AATCCCGTGTAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGG
    AAGGCTCACCAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAG
    CCATAGGGGCTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACG
    CCTTTCATCTGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAA
    TATAAGGACATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTG
    AGTAACTGCTAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGA
    CTCTAAGTCATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTG
    ACTAATACAGAAGTTGGTACCAGGAGTGGTTCTAGAGTAACAGAAGTACAAGGATGAATCTTTTAAAATA
    CTGGAATTGGCTTGTTGATCCACCAGCACTTTCAACTATTGAAACCTCTCCAGATTCTCTCCCTCCTGGG
    AGCTCAGAGAATTTTGAAGACCCATGGTTGAAACTATATTCCGAACTTAAAGAAGCAAATGCCCTTGATT
    TTCTTAATGAATTAGGTGATTCAGTGCACAAAGCTTTCTACAAGATGGGGAAAAAATTGGAAAATGATTT
    TACTGGCTGGCTGCTCTTAGTATCTGTGGAAAAAATGATGAATGAAAGGAAGGAGTTGTGTGATAAAATC
    GAAAGGCTCCAGACACAAGTAAACGATCTAAAAGTTGCTAAGTGTGTCCTTGAGGAGAATCTTCTCTCTT
    GTAGCAATAGAGCTCAAGTTGCAGAAAATCAAACAGAAACTCTCATTGTAAGGTTGGCTGAACTACAGCG
    AAAATTCAAGTCTCAGCCTCAGAGTGTGTCAACAGTTAAAGTAAGGGCTCTAATTGGCAAAGAATGGGAT
    CCTACAACATGGGACGGGGATGTGTGGGAAGACCATGTTGAAGCTGAGAATTTTGAATCTTCAGATTCTC
    AAGGGTTTGCCCCACCTGAGGAAGTAGTACCCTCAGCCCCACCCCTTGAAATAATGCCTTCCCCACATGA
    GGAAATTAATTTTGCAGAGTCTGATAAACCAGCAATGATTTTCACTACTGATGTTTCTCAAGGCCCACCA
    ATAGTTTCTTCTAGACCTGTAACCAGACTCAAAGCAAAACAGGCTCCTAGAGGGGAGGTAGAAAGTGTAG
    TCCATGAGGAAATTCGCTACACTACTAAGGAGCTTAATGAGTTTGCTAATTCATTCAAGCAGAAACCTGG
    TGAATATGTGTGGGAATGGATTTTAAGGGTGTGGGATAAGGGTGGAAGGAACATAAGACTAGAGCAGGCT
    GAGTTTATTGACATGGGTCCTCTGAGTAGAGATTCTAGGTTTAATACGGAAGCTCGCATAATTAAAAAAG
    GTGTCAAAAGTTTGTTTGAATGGTTGGCTGAGGTGTTTATCAAAAGATGGCCTACTGGAAATGACTTGGA
    GATGCCTGATATTCCGTGGCTTAGTGTTGATGAAGGGATTTTAAGACTTAGGGAAATTGCAATGCTAGAG
    TGGATATATTGTGTAAAGCATAATTGTCCACAATGGGAAGGTCCAGAAGATATGCCTTTCACTAGCTCTA
    TAAGACGCAAATTGGTGAGAGGGGCACCAGCACATTTGAAGGGTTTTGTTCTTTCCCTTTTCCTTGTACC
    AGATCTTAGCATTGGAGATGCTTCTGCTCAATTAGATGAATTAAATTCACTGGGTTTAGTTGGATTCCGA
    GGTAACAAGGGCCAGGTGGCAGCATTGAATCGCCGGAGACAAGGTGATTCTAGTTATTATAATGGACAGC
    GTAGACAAAAGAATGTTTATAATAACATACCCAGCAATGGTCAGCACAGGAGAGGTGAAATTTATAATGG
    CATGACTCGGTTGGACCTTTGGTACTGGCTAACCAATCATGGTGTTTCCAGGAATGAAATACATAGGAAG
    CCTACTGCATATTTGTTTGATCTGTATAAGCAGAAAAATTCTCAAACAAATGAAAGAAAGGCTACATTAG
    ATCATGGTAAACAGCAATCTCGGCCAGTGAATCAATTTCCAGACTTGAGACAGTTTGCAGATCCAGAACC
    CCTTGAATGAAGGGGTGGCCAGGTTCCGCTGAGGAAGGATCTTGATAAGACACCCAAAGGTTTTGCTGTT
    ACCCTTTCTCCAGTTCTTCCCCAGAGGGACCTAAGGCCTTTTACAAGGGTAACTGTACACTGGGGAAAAG
    GAAATAATCAGACTTTTCAGGGTCTGCTGGATACTGGTTCTGAGTTGACACTGATTCCAGGGGATCCCAA
    GAAACATTGTGGCCCTCCAGTTAAAGTAGAGGCTTATGGAGGGCAGGTGATTAATGGAGTTTTGACTGAT
    GTCCGACTCACAATAGGTCCAGTAGGTCCCCAGACACATCCTGTGGTGATTTCCCCAGTTCCAGAATGTA
    TAATTGGGATAGATATACTCAGAAATTGGCAGAATTCTCATATTGGTTCCCTGAACTGTAGAGTGAGGGC
    TATTATGGTTGGAAAGGCCAAATGGAAACCTTTAGAGTTGCCTCTGCCAAAGAAAATAGTGAATCAAAAA
    CAGTATCGTATTCCTGGAGGCATTGCAGAAATTACTGCCACTATCAAGGACTTGAAAGATGCAGGGGTGG
    TGGTTCCCACCACATCTCCGTTTAACTCTCCTATCTGGCCAGTGCAGAAAACAGATGGATCATGGAGAAT
    GACAGTTGATTATCGAAAACTAAATCAGGTAGTAACTCCAATTGCAGCTGCTGTACCAGATGTAGTTTCA
    TTACTTGAGCAAATTAACACATCTCCTGGCACCTGGTATGCGGCTATTGATCTGGCAAATGCCTTCTTCT
    CAGTACCTGTCCATAAGGACCACCAGAAGCAATTTGCTTTCAGTTGGCAAGGCCAACAGTATACTTTCAC
    AGTTTTGCCTCAAGGATATATTAACTCTCCTGCCCTGTGTCATAATTTAGTTAGAAGGGATCTTGATCGT
    TTGGATCTTCCACAAAATATCACATTGGTGCACTATATTGATGACATTATGCTGATTGGACCAAGTGAGC
    AGGAAGTAGCAACCACTTTGGACTCATTGGTAACACATATGCGTATCAGAGGATGGGAAATAAATCCAAC
    CAAAATTCAAGGACCATCTACCTCAGTGAAATTCTTAGGAGTCCAGTGGTGTGGAGCATGCAGAGATATT
    CCTTCTAAGGTGAAAGATAAGTTATTGCACCTGGCCCCTCCTACAACCAAGAAAGAAGCACAACGTTTAG
    TGGGTCTATTTGGATTCTGGAGACAACACATCCCTCACTTGGGTGTGTTACTTAGGCCTATTTACCAAGT
    GACTCGGAAAGCTGCTAGCTTTGTGTGGGGCCTGGAACAGGAGAAGGCCCTTCAACAGGTCCAGGCTGCT
    GTGCAGGCTGCACTACCACTTGGACCATATGACCCAGCAGACCCGATGGTACTTGAGGTGTCTGTGGCTG
    ATAGAGATGCTGTTTGGAGCCTCTGGCAGGCCCCTGTAGGTGAATCACAGAAAAGACCTTTGGGATTTTG
    GAGCAAAGCTCTACCATCATCTGCAGACAACTATTCTCCCTTTGAAAAACAGCTCTTGGCCTGCTATTGG
    GCCTTAGTGGAAACTGAACGTTTGACAATAGGACACCAAGTTACTATGCGACCTGAACTACCCATCATGA
    GCTGGGTACTATCAGACCCTGCAAGTCATAAAGTGGGACGCGCACAGCAGCAGTCTGTTATCAAATGGAA
    GTGGTATATACGTGATCGGGCCAGAGCAGGTCCTGAAGGCACAAGCAAGTTACATGAAGAAGTTGCTCAA
    ATGCCTATGGTTTCTACTCCTGTTACACTGCCATCTGCTGCCAAACATGTGCCTATAGCCTCATGGGGTG
    TTCCCTATGATCGACTGACCGAAGAGGAAAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTTATGC
    AGGCACCACCCAGAAGTGGACAGACAGCTGCAGCATTACAACCCCTTTCTGGGACAACCCTGAAAGACAC
    AGGTGAAGGGAAATCTTCACAGTGGGCAGAACTTCGGGCAGTACACATGGTATTACAGTTTGTTTGCAAG
    AAGAAATGGCCAGATGTACGATTATTCACTGACTCATGGGCTGTAGCCAATGGATTGGCTGGATGGTCAG
    GGACTTGGAAAGATCACAATTGGAAAATTGGTGAGAAAGACATCTGGGGAAGAAGTATGTGGATAGATCT
    CTCCAAATGGGCAAAGGATGTGAAGATATTTGTGTCCCATGTAAATGCTCACCAAAAGGTGACTTCAGCT
    GAGGAGGAGTTCAATAATCAAGTGGATAAGATGACCCGTTCTGTGGACAGTCAGCCTCTCTCCCCAGCCA
    TCCCTGTCATTGCTCAATGGGCACATGAACAAAGTGGCCATGGTGGTCGAGATGGAGGTTATGCTTGGGC
    TCAGCAACACGGGCTTCCACTCACCAAGGCTGACCTGGCTACAGCTGCTGCTGATTGCCAGATCTGCCAA
    CAGCAGAAACCAACACTGAGTCCCAGATATGGCACCATTCCTCGAGGTGACCAGCCAGCAACCTGGTGGC
    AGGTTGACTACATTGGACCACTTCCTTCGTGGAAAGGACAGCGTTTTGTTCTTACTGGAGTAGATACTTA
    TTCTGGTTATGGATTTGCCTTTCCTGTACGTAATGCCTCTGCTAAAACCACCATTAACGGACTGACAGAA
    TGCCTTATCTATCGTCATGGTATTCCACACAGTATTGCTTCTGACCAAGGAACTCATTTCACAGCCAGAG
    AAGTACGACAGTGGGCCCACGATCATGGAATTCACTGGTCTTACCACATTCCCCATCATCCTGAAGCAGC
    TGGTCTGATAGAAAGATGGAATGGCCTTCTGAAGACGCAGTTACAGCGCCAATTAGGTGGTAACAGCTTG
    GAAGGCTGGGGTAGAGTTCTTCAGAAGGCAGTATATGCTTTGAATCAGCGCTCGATATATAGTACAGTTT
    CACCCATAGCCAGGATTCATGGGTCCAGGAATCAAGGGGTGGAAAAAGGAATAGTTCCACTTACTATCAC
    TCCTAGTGACCCTCTAGGAAAATTTTTGCTTCCTGTCCCCATAACTCTAGGTTCTGCTGGCTTAGAAGTT
    TTGGCTCCAGAGAGGGGAGTGCTCCTACCAGGAGCTACAACAAACATTCCATTGAACTGGAAGCTCAGAC
    TTCCCCCTGGTCATTTTGGGCTTCTAATGCCCTTAAACCAACAGGCTAAAAAAGGAGTAACAGTGTTAGG
    AGGGGTGATAGATCCAGATTACCATGGGGAAATTGGATTACCTCTTCACAATGGTGGTAAGCAAGATTAT
    GTCTGGAGTGTAGGAGATCCCTTAGGGCGTCTCTTAGTACTACCATGTCCTGTGATTAAAGTCAATGGGA
    AACTACAACAGCCTAATCCAAGCAGGATGACAAAGGACACAGACCCATCAGGAATGAAGGTATGGGTCAA
    TCCTCCAGGAAAAGAGCCAAGACCTGCTGAGGTGCTGACTGAAGGAGAAGGAAATATAGAATGGGTAGTA
    GAGGAAGGTAGTTATAAATACCAATTAAGGCCACGTAACCAGTTGCAGAAACGAGGATTATAAAGTAATA
    TGAATGCCCATTGTAAATTTACTAATGCGTTTGCGATTGTACGAGGGATAGTTATATCATGTTAGGCGTA
    TTTACAAACTTGTTATTGTTTTATGTGAACATGAGATATTATTTGTGTCAAGTTGACAAGGGGTGGATTG
    TAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGGAAGGCTCAC
    CAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAGCCATAGTGG
    CTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACGCCTTTCATC
    TGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAATATAAGGAC
    ATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTGAGTAACTAC
    TAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGACTCTAAGTC
    ATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTGACTAATACA
    TCCCTACATACAGGAAACTTGATGTATAAGTAAAGAAAATAGTAAATCTAAAAATTTCCTTATCAAATAC
    ATGCAAGAAATCCAAAATACCATGAAAAGACCTGATCTAAAAATAGTAGGAACTGAAGGTGAAGTGTCCC
    AGCTCTGAAAACTAGAAAATGTTTTCAATATTTTTCCTAACAGTGAAGACATGGATCTAATGAGGCCACC
    TCTTATAGCCATGCAAGACTGACAGTGGAGGGATAAGGACACCAATCCACCCACAAAACTTTTGACCCTA
    AATCAGTTTTATCTAAAAGCAATGCAGGGGCACAAATGGAGCAGAGACTGGAGGAATGGTCAAACAATAA
    CCTGTCATATCTGAGACCCACCTAATTGGCATACACCAGTCACTGACATTATTAATAATGTTCCATTATG
    CTTACAGGCATTTCTCTAGCATTACTATTCTTGGAGAGACTTCACACAGCATCTTATTGAAACAGATGCA
    GACACCCAGAACCAAACATTGGATGGAGATTGGGATCCTTAAAGAAGAGTTGGGAGGATAATTGAGAGAC
    CTCAAAGGAATAGCAACCCCATAGAAAGACACGAACAGGGTCAGTAAACCTGGACCCATGGGGTATCTCA
    GAGACTGATCACCAACCAAAAATCACAGAGGGCCTGGATTGATTCCCCTGGCACACATGTAGCAGAGGTC
    TGCCTTGTCCTTCAGTAGGTGAAGATGAGCCTAAGGCTTCAGAGACTTGATATATCAGCATACAGCAACA
    CCCAAGTGTGTCCCACTATCTCAGAGGTGAAGGGGTGGAGATGGGCAGAAGGGTCCTGATAGGGGACAAC
    TGCAAAGAGGCAATATTTGGGACAGAAAGAAAGGAAGAGAGAGGAAGATAAAGAGAGAGATGAAGGAAGG
    AAACAAACAAACCAAGATGAAGAGAGAGAAAAGGGGGAAAATAGCCTTGAATGGTTCAGGGATTCAAAGG
    GACATACCTAAGCATGGTAAAACAATGCACATAATGGCAGCAAGTAGCTAACACTGAATTAAATAGAAAG
    ACACATAAAGCAGTTCTACTAAAGTAAGAGACAGAGTTGCCCAATCTCACCCTATTTATTCAATAATAAT
    GTACTTGAAGTTCTAGCTAGGGCTGTAAGACAACTCAAGGAGATCGGAGGATACAAATTAGAAAGAAAAA
    TTCAAAGAATTGTAATTGGTAGATGATAGGATAATATACATAAATGACCTCAAAATTCTTCCAGAGAACT
    CTGAAAGCTGATAAATACCTTCAGCAAAGTGGCTAGAAACAAAATTACTTCATAGAATTCAGGAGCTATC
    CTTTATAGAAAAGTTAAAGTGGCTGAGAAAGAAATTAGGAAAATCACATTCTTTGCAATAACCAAAAATA
    ATATGAATTAGCTTGGTGTGACTCTAATCTTGCAAGTGAAAGATGTGTGTGACAAGAGCTTCAGGACCCT
    GAAGAAAGAAATCAAAGAACTCAAAGATGCAAAGTACTCTAATGCTCATGGATAGGCAGAATTAACATAA
    TGAAAATGCCAAATTTAACAATTCAATCTACAGATTCAGTGGAATTCCCATTAAATATCCAACCCAATTT
    ATTACATTCTTGAAAAAGCAAATCTCAACTTCATATAGAAAAACAAGAAATTGAGGGTAGCTAACAAAAT
    CCTGAACAATGAAAACACTTCAGGAGAATTCACCATCCCCTACCTCAAGCTGTATTTTAGAGCAATAGTT
    ATTAAAACTGCATGGTATTTGTATAGAAACAGATATGATGATCAATGTAATTGAATTGAATACACTGACA
    TAAAACCACACTGTTATGGACACTTGATTTTTGACAAAGAACCCAATAATCATAATAAAAATCATAATAA
    GAATGCATCCCCCAACAAATGGTCCTGATCTAAATGCAAATAGATTCATATCTATCATCCTGCACGAAAT
    TCAAATCAAAGTGAATTGCAGACTTCGACCTAATACTGGATTAACTAAATCTAAATGAACAAAAAGTAGA
    GAATAGTTTTGAACTCATTGATGCAGGATTCAATTTCCTGAACAGAACCACAATGGCTCAGGCTCTAGGA
    TCATAAATTGGTAGATAGGATCTCATGAAACTGAAAAACTTCTGTAAGGCAAAGGCAATAAAACAAAATG
    GCAACCTACAGATTGGAAAAAAAATAATCTTCAGTATCCCTAAATCCCATTGAAGGCCAATATGCAAAGT
    ATATAAAGAACTCAAGATGTTATCCTCCACAAAACCAAATAAATCAATTAAAAATGAACTACAGAGCTAA
    CAAGAGAATTCTCAACAGAGGAATCACCAATGACTGAGAATCACTTAAAGAAACATTCAATATTTGTAGT
    CACCAGAGAATTGCAAATCAAAACTACCCTGAGATTCTACTTTATACCAATCATAATGGTCAAGATAAAA
    ATTCAAATGACAGCGCATGTTGGTGAGGATGTGTATACTTTTGCATTGCTGGTGAGGAAAACAATCTGGC
    AGCTCCTCAGAAAATTGTAAATAATTCTACCTGAAGATCCAGCCATACCACTCCTGTGCATATACATAAA
    ATGTGCTCCACCATACCACTAGGAGATATGTGCCACTATGCCCATAGCAGCCTTATTTGTTAATAGCCAA
    AAGATGGATACAACCCTGATGTGCCTCAAACAAAGATTGTATATAGAAAATATGGGTTCCCTTACACAAT
    GGTATTCTACTCAGCAATTAAAACGTGAGAACATCATAAAGTTTTCAGGCAAACTGATCAAATGAAAAAG
    TATAATCTTGAGTGAGGTAACAGAACCAAAATGACCTGCATGGGATGTACTCACTGGTAAGTGAATATTA
    GACTAAAAGTATAGAATATCAATGATGAAAGCCACAGACCATAAAGAGTTTAATAAGATGGAAAGCTCAA
    GTACGGATTCTTCAATCCAATATAGAAAGAGGGACAAAATAATCAGGGGAGGCAGAGGGAGAGAGAGACA
    TTTGGTGGAAATGGGAGAAGAAGGGAGAAAGGCAGAAAGGATCAGGCGTTGGGGAACACTGGAAAGCAGC
    CCAGGGGTCCTGGTAAATGGATTAAATATTCAGCTGAATGGAGGTGGGAGGCAGGGGGAAGCTCTGCAAA
    GTCCAAGAGACCTGGGATGTGAGAGGCTCCCCAGATGATAATCTTAGCCTTCATGCCTAACAGTTGTAGA
    TAAACCCTGAAGAGATCACTTCCAATAGATGCAGAGGGCCCTAAGTGGTTGGATGGAGTCCCCCAACTTA
    CCTCAAAATTGTCAATACTCATAGAAATTAGGACAATGAAAATCAAAACACCCTGAGATTGTATCTTATG
    TCTGTCACAATGGTTAAGACCAAAACCTCAAGTGATGGCTTGTGCTGGCAAAGATTCAGAATAGTAAAAG
    TCTCCCCATGGCTTGTGGGAATGCAAACTCTTACAACCTCTTTGGAAGTATATTTGATTGTTACCTGGTA
    TAAAATGGCAAAAAAAAAAAATGGTTGAAGGGGGACTAAAAAAGGAAGAAAGGGGAGAACTATGGGATGC
    GAAACAAGAAAGTTTGTTGCAAAAGAAATATGTTTCCACTGCAAACCCTGAGTCTCAGACAGAAGGGGAC
    CTGGAATTCTTCAGATACAAAGAATCTCTAAACCCTGAGGACATTCTATCACAAATAAGTAAAATTCAGA
    AAATTCTGAGTGCTCCCATCACGGAGATGAATCTGCTATGAACAGCTCATAGGTGTGACCCTCTACAAAA
    GCCATATTATTGAAAAGCCACATTGTGCCCAGACTTTGGAAAGACTGAGCTCATATCCTGAAATACAGTT
    ATGTGTGGTTCTATTTAATTACACATTTACACTAAGAAAACATGGCAGTATGGGAATGAAGCTTGTTCTG
    TACACATTAACAGAGGGAAACTAAACAAAGTATGGTGAATCCCTAACCAAAAGTAAAAAAAAAAAAAGAA
    AGAAAAGAAAATAAAAGTGAAACTACAATATGTTTCAAATGCTGTAACTGAAATCTGGTTTTTTGATGCT
    TATATCTGGTATCATCAGTGACTTCAGATTTAGTCCAACTCCAGAGCATGGTATAGCAGAAAGACATGCA
    AATAAGCCTTCTCTCTGCCCATGAAAAACACCTCGGCCCTGACCCTGCAGCTCTGACAGAGGAGGCCAGT
    CCTGGATTCGATTCCCAGTTCCTCACATTCAGTGATCAGCACTGAACACGGACCCCTCACCATGAACTTG
    GGGCTCAGCTTGATTTTCCTTGTCCTTGTTTTAAAAGGTAATTTATTGAGAAGAGATGACATCTGTTGTA
    TGCTCATGAGACAGAAAAATTGTTTGTTTTGTTAGTGACAGTTTTCCAACCAGTATTCTCTGTTTGCAGG
    TGTCCAGTGTGAAGTGAAGCTGGTGGAGTCTGGGGGAGGCTTAGTGCAGCCTGGAGGGTCCCTGAAACTC
    TCCTGTGCAACCTCTGGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCAGAGAAGA
    GGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTATTATCCAGACACTGTAAAGGGCCG
    ATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCCGTCTGAAGTCTGAGGAC
    ACAGCCATGTATTACTGTGCAAGACACACAATGAGGAAATGTTACTGTGAGCTCAAACTAAAACCTCCTG
    CAGAGCACCCAGGACCAGCAGGGGGCGCAGAGAGCACATGGAGTTCTGATTCACAGAAGAGTTACAGCCT
    GTACAATTAGACCCAATCTTCAACAAACCGTCAAAATATTCGATCCAAAATTGTTCCTGTCTAAAAGTAA
    TTCAAGGACAAAATGGACCAGAGACTGAAGAAATGGCTGACCTGTGACCCTCCCAACTTTGGATCTATCT
    CATAGGCAGGTACCAAACCTTGACATTTGTCACTGACACTGTATTGTGCTTGCAGACAGGAGCATAGCAT
    GGCTGACCTCTAAGAGGCTCTGCAAGCACCTGAATGAGACAGATGTAAATAGAGTGTACAACTGGATTAA
    GTTTGAAGACTGCAATGGAAGAGTTAGAGGAAGGAATGAAAAATCTGAAGGGGATCACAACCCCAGAAGA
    AGACCAACAGTGCCAATTAAACTGGATGCCTGGGAACTCCCAGAGACTAAGCCACAAACCAAGAATCATG
    CAGGCTGGTCTGAGAATCCTGGCACCCAAGTCTCAAGGAACTGCCTTTTCTTGGCTCAGATGGGAGAAGA
    TGAGCCTAAAGCTTTAGACTTGACGCCCCAGGGATGGGCAATACAAAGTTGAGTTTCTTGGGAAAGTGAA
    GAGGATGGTGGGAGGAGGAATAAATCTGGGAAGTGGGACTGGTTGGGGACAACATATTGGTCCTAAATTT
    ATGAAATAATTATCTAATTGATAAAAAGAGTCCTTGGGGTAATGGAGGGCTAGACTCCTCTGTGCCTAGT
    TTGTAACTCTACAGGGATCCTCTTTAAAAGAATAGGGTGCACATAGAGTATATGTGTGTGACACTAATAC
    AGGGGTAAGTGTTTCTGTAAGAACTTTATGAATAAACTTTATTAAAACATCAAAAAGTATAGGTTGTAGC
    AACCCTTGACCTGTACGAATGTTTATAAAACTTTCTATTTTCCTTAATTATATCTGTTTTGCATTTGTTT
    ATTTATTTATTTACTTACTTACTTATTTATGATTTCACTGTGTCTTTCTGTAGTCCTGTATGTGTTTATT
    TGTCTCTTTATTGCTCTGTTTCTGTTTCTTTCTCTCTGTCTAGTATTGTATCTCTCTGTGTCTCTATGTC
    GCCGATTGTGTCTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATATTGAAA
    TGAGATCTCTTCTTCTATCAGTGTACATAATGACTTTGAAATAAAGTCCATCACTGGGCTTGGATATCAC
    TATCTTGTTAGAATGGTTGCCCAAAGTGTTCCAACCATCACACTGTTTCTACATATAATAGATGGGTTTA
    TTGATTTACTACCAAAACTACCCCATCTTAAGTTATGGATATTAAACACAGATCCTCATGGTGTGTTAAG
    AACTTGAAGCACAGAGCTATATGTACAGCCCACTAAAAATGACTTAAATATAAGATAAACTCTGGCTTGA
    ACAAAACACAGAGCATCACACGAGGCAGAAGTGAATCTCTATGAGGACCAGGATCACATCCCAAAGAACC
    AAAGAACACTTGAGACTGTGGAACCCTAGAGTCAGGGCTGACACTCAGTAAGATTTACTTCATAGAGCAT
    GATGTGGGATTCAAGAAGAAATCAGGCAGGGCCTGTGACCCATGAACCAGATGACTTCCAGTCTCTCCAT
    CATCCTCACACAAACCATAGGCAACAGCTCAGCACTGACTCACCTGTGATGCTGGTGACAAAATGTGTGC
    AAAGCCTCAGAACTGAGACGTGAGGCCAATGTTGCTACCGACACTCAGTGAGCTTCAATTTGCTGCAAAC
    TGCTCCAAAACAACTCAAAGGGTAACTTCTTCTTTTTCTATTTGTACTTGTGATGCCTTGGTAGATATAG
    TTCCCCAATAATTTAATAGGACAAATCATTTTTCCTTTTAACACATTAATCCCCTTTCCTTCCCCCAAGA
    TTCTAAAATTTTTCTTTAACACTGATGTCTGAGAGATATGGCATAATAGATGTTTGTCTTATTCTGAGTT
    GTTCCTTATCAAAAGAAATCAGCTGTGGCACTTTGAACCTAAATATAACAATATTTCACAAAGGCATTCT
    GGGAATATATAGATGGTATTGCTACAAAAGAATAGGAGGATCTATGGCCATACCACCCTTAATGAACCAG
    ATACTATCTAAAAGATTAGGACAGCTGGGCATGTTGGCACAGGCCTTTAATCCCAGCACTAAGGAGGCAG
    AGGCAGGCAGATTTCTGATTCGAGGCCAGACTGGACTACAAAGTGAGTTCCAAGACAACCAGGGCTATAC
    AGAGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAGAGTAGGAGGGTATTAGATGCGAGGTGAT
    GATGGCCATTCAATCAAACCAGAATGTGACCTGAGAAAAGGTCTAAGTTTGTCAGCTTGTCCTGGAGATG
    TGTTGCCTCTGATAAATGCAGAGCTGGAACTGCAATACATTGGATTCCTGTGAGTTACGGAGAAATAAAA
    GGAATAGCCTCTGTTTCCTTTGGTTCATCAAGTTGGCAGTGAGCTAGAAGATGATACTGGGAACCATCAC
    ATGTACTTCTACAGAGTTATTCTACATTCTACAAGTCAGTCTTGCTTGCTTTCGTTTCTGTGAGGAGCTA
    AATACAGAATGTTTTCAATGTCTCATAATGTTCTGTGCACACCCATGGGTGCCTAGCCAAGGATAGAAAC
    TTGTATCTGATAAGGAAAGAGGATGCTGCAGTGGCCACACTCTGAGATCACCTCCTGCCAAATTATACTA
    GAGTGTTTTACATAGTCTTGAAAACACTAGGTCAAATATGTTGTCATCCAGATATGAAAACTCTGTGCAA
    CAATTCAGAAATACAGTGGTCAGGACAACTGGTATGTAACACTTTAGTTCCAGCCCACATTAATCTCACA
    TCTTTTCTGTGGTTTCAATAAAGAGCACACATGTTTGGAACACCATCTCCACTTTGTATGCTTATCCTTA
    TGCATCAAACTTTTCCTGTAATTTCCTACCCCGCACAGGTTCCTGTCTTGATCTCTTTTTCTAATGAACA
    GCAATACAGAAGTGTTGGCCAAATGAACACTTTCACCCCAAGTTGCTTTGCCATTGTGTTTCATCACAGC
    ATTAGTAACTCCCAGTAAGGCTACTGGTTACCCTGCATCTTCTATGTAGATCTTTTGATGAAGTTCCAGG
    CTTGGGGTCTTTATCTGACTGTTATCCTGGTTAATTCATGTCCACCAATAGAATATTGTTCTCATTTTCA
    ATATCATATGAAGTTACCTGTTTATAGGATCTTCCTAAAGCAGAAAACTATATAAAATGTCTGCTTACTT
    TAAGAAAAGTATGTGTCCTTGGAGGTAAATGGAGGAAAGGGTGGAGGATGTTGAGTAATGGAAGACTGGC
    TAATACAGGAAGGTCACAGAATTGAATGATTTTAAAAATTTTAACTCCTCATCAATGAGGTACTTCAAGT
    TGAGGCAGGAGTCCTTGATGCACAGGTGACCCAAAGGCCCCAGCCTCTGTTTCTTGGTCTGCTCCAGTCT
    CCCGGCTCAGCACAGTTCACTGAGTGACAATCTTTAAGATAGTCTTTCAGGATACAGTTATCACAGTTTT
    CAAACTCTCTTCATTGAAACAATATAATGAGTCAGAGTCCAGTGTGCCATTAATAGGCCCTATGCAATAG
    GATTTTACTTCATCTGTGGAAAGAAACCACCATGGCCTCATCAAGGCCTCATTTGATGATTAGTGTAGAA
    AGCGTCCCCAGAGCTTTGCTGGGATGAAAACCCTGGCAAGATGTTTAGGGCACTCTCCATCTGACAGGAC
    AATTCTATCAAGGGTTGCTTCCATATAGAGAGAAATCTCCACATCTGCAGTATTTCCCGATTTATCAACA
    GTTCTTTACCCCGTGTCATATGCATAAAATCTTTTCTCTGTAGAATTTTCAGTACTAAAAACTAACAAGG
    ATGTTGGTCCTGATATCAGACATAATAAATAAATAAACATCAAATTATACCCTAAACATGTGGAGATGGT
    TTTCTGTTATGAATTTATTGGCTCTCTAAAAAGTGTTAATTAAGCCAGGCGTGATGGCACATGCCTTTAA
    TCCCAGCACTTGTGAGGCAGAGGCAGGCGAATTTCTGAGTTTGAGGCCAAACTGATCTACAGAGTGATTT
    CCAGGACAGCCAGGGTTACACAAAAAAACCCTGTCTCCAAAAAGTAACAAAAAGAAAGTGTTAATTAACA
    TCTACACTTTAAGATAATTTGGTGGCAAAGGTTAATAGTCTGAAATACTAGATATGAAAGTATGACACCT
    AAATTGCATGCAGAGAATAAAGCTGAATTCCAATGAGATTCATGCCTTGTGAATCCATTCCAATCTGATC
    TCAATCTTCAAACATGTTTGTGCATAATTTACATCTTAATCAGTTGCTGGAAATTAAAAGGACATAAGCT
    AAACTTAAAACTCTTTCTATTTAATGTGTCAGAATAGAATATGTGCACTGAAGTTTTCATGTGCATAGGA
    TATCATTGTTACAATGATGGTTCAAATACGCACCAGTAGCACAGGTGATGCTTGACATGAAAAAAACTGT
    GACCAGAGCCCAGACGTTGATGTTGGCTGAAAGAGCCTAGTGTCTCACATTTGATCAAGCAAAGTGTTAT
    TCAGAGAAGCAGTGCCAGATTTGGGATCCTTCTTAATGGATCAAGAACATGAGGAAGATTTGGAAGCCAG
    ATTGATGAGATTACTAGAATCCTGGATCTGAACACACAACACACAATAGTAAGTTGAACTTTTCCACGCA
    ATGCTAAAGATAAAGCATCCAGTATCATGTTATACAGGACCAAGGTGAGGATCAGTCTGTGGGTGAAGTA
    CAATGAACTGGAAATTGTTTGGATTTTAAATGTTGGTTTTTAAATTATATATAAAATATATATATATATA
    TATATATATACATATATATATATATATATATATATATATATATATCGGAACCAACATTTAAAATTTGGAT
    TTTAAATGTTGGTTTTTAAATTATATATAAATTATATATATATATATATATATATATATATATATATATA
    TATATATATATATATCGGAACCAACATTTAAATTCCTAATTCTTAAGGACTTCACTTTTGACCAAAAATA
    TTTGAAATGGGTTATTTAGGTCTGTGGTGGTTTGAATATGCATAGCCTATAGGAAGTGGAAGTATGGCCA
    TTTTGTAGTATGTGTAGCCTTGATGGAGGAAGTACATCACTATATGGTGTTGTGAGAGTTGAGATCCTAT
    GTTCAAGTTCTGCCAAAAACAGAAGAGAGTCCCCTCCTGGATATTTGAAACAGATGCAGAGGCCCAAAGC
    CAAACATTGGGTATATCTCAGGGTTTCTTATGGAAAAGTTGGCTGAAGGATTGAAGGCCTTAAAGGGGAT
    AGGAACTTCACAGGAAAACCAACAGATTCATCTAACCTAGACTCTAGGGGTCTTTCAAAGGTTGAGTCAC
    CATACAAAAAGGACACAATGGCTAGACCTAGGTCCCTGATACATATGTAGCAAATGTGCAGCTTAGACTC
    CATGTGGATTCCCCAACGACTAGAGCAGGTATTGTCCCTGAAGTTATTGCTTGTCTGTAGAATACATTTC
    CCAACATAATTGTCTTGTCTGGCCTCAGTAGGAGAGTATGTTCCAATCCTGGAGAGACTTGATATGCCAG
    GATTGAGAGATTAAGGGTCTCCCACCATCTCAAAGTTCATGGGTATGACAGGGGAGGGGCAGTGCAGGTT
    TTCCCCATCTCTGATTTCCAGCATTTGCACCCCCAATATTGAGGGGGAGGTCTAGCAATTGGAGGGGGGA
    CAAACAGTATGAGAAAGCTAACTTTAGTGGTGTTGTCACAGTGTGGGGTATTGTGGGTGGGAGTGGAGAT
    GAATCCTCCTCCTCCTCAGGAGGCTGTAGCAGGTGCTTGCCCTCTTTTGGGACAGTAGAGCTGTGGTCAG
    TACTGGCCTGTTCCTGACAAATTTATTCTGTGCTGTGACCATTTATTCAAGGGAGGAAGATTTAAGACTT
    GCTATGATAACCTGACGAATCTGGACAAACAAGAGACAGGAGGTAACACCTGAGTTACTCTTTTGCTTGC
    TAGTACAGAAGAGCACTATAATCCACAAATTGGGGTCCCACATATTCTTGAGTGGGATCCAAGGTGCCAT
    AATTTCAGCCACATTCCAAAGTCCCTCAAGGTCTGACAGTTTAGTCTTTAGGCTTCTCCTAACCAAATCA
    TATTTGGAGTTCTTTAACATAAAAGGGTAGTTTCCCCATATCACTGCCAGCAAAACAGGTTGAATTTGGA
    CAGACAAGCAAAACTATCACAAACAAGACAGACTATGTGTGCGATCCAAATGTCTTGTGATTAGAGGGGA
    AGTTCTGCAGCTTATTAGGCTGTGTCCTTATGCCCACAAATGTCTTCAGATAGGAAAGGGCCATTGGGTG
    CCAATCCTTAGTTCTTCTGTTTCCTGGGCTGTCAGTTTTTATGTGGAGAAGACATGAACCAAATGCAAAG
    TCATGTATCCTACAAGAACTGACATACACAGACAAACAAGGAATAACAATGAGCTCTCTCATTCACCTAA
    GGGGGTCTTATAACATAGAACAGAAAGGGGCCAACTTAGCAAGAATTGACCAAAAGGTGGTCACCTAGAC
    CACACCACAATGCAGGAAGGATATCAATGAAGGAAGAACAAAGGAAAGGCCAAATCAAGTTTGTTGGCAC
    ATGACATAAAACAGTGCTCAAAAAACAATAGATGCGAGCAAAATGAAGCATTGGAATCATATCGGGGTAC
    ATAGGGGTCACTCTTACCCCTGTTCAAGTGCCAGATGTTGTTTTAGCCTTAGGCTCCCTGAGTTTGTCTT
    TAGGGGAAATGGGTAAGAATACACAAAAATAATGTCACAGAATCTTAGAGGCAGATGAGAAGTCAAAGCA
    GACTCATTTATTGCTATAGAAAAGAATGCCTTTATACCTTCTGTCCATAACACCTCTGCCCATATATGCT
    CATCTCTGTGAGGCAATGGACTACCTGCAGACAGGCTGGTATCCAGTCAAGCTGAGGTCTGAACCACAAT
    GGTCGTAACTCACCTAAAGGACGTGGTAGGCATGACGCGATAGGCGTTCCCTCATGCTCCTGGAACTCCG
    GCCCCTGCCTAAGGTACCACCTCCCACAGCCCCCACAAGAGAAGCATGGTCAGTAGTCATGTAAGCAATG
    GCCCAAGCTTCTGACCTTCAGCCTAAACTCCTCCCCAGTTACCTAGCAACAGTGAAGACCATAAAATGGG
    ATGTTAGGCCCCCACCTCACTCTCTTACTCCTTTGTTCCTTTACCTCTCACTTCTCTCAATTCTCTCTCC
    TCTTCCCTTTCTTTGTCTTCTTCTTTCCTTGTCTCTCTGTCTTTCTCTTTCTCTCTAGCCTTCTCTGCCT
    CTCTCTCTCTCTCTGTCTCTCTGTCTCTCTCTTCTCTCTTTCCCTTGAATTTCTTTTTTTCTTTTTTCTT
    TTTTTGATGAGCACAGTATAAATAGTTTATTGCATTGTTTCTGAATGTGGCTGTATTCTCTATTGTTCTC
    TTTCTTATTTTTTTTCTTTTTTTGGTTGTTTTTGTTTTTATAGTCTCGCACAGAATTCTACATCTACCAA
    ATCCCCATTGTGTACTTTAGTTTTGTCTTGTATTCAAAGGTTCTTTCAGAAAGAATCTTAAAGATGAAAT
    TTGCCTTCCAACTTTTCAAAAATACATATAAAGATAGATGTTACATAGTTCACGGAATAGACACATATAC
    GGAGATGCAGCAATATCTCCTTTGTGCCTCTTGTATATTCATGTTATTTCCTCAACTAAAAATACATAGC
    CACAAAGACATACAATGAGCACTCCACTTAGGAAAATATGGCCATATTTTTTTACATTTTTATGATTTTA
    TGATTAGGTATATTCTTCATTTACATTTCCAATGCTACCCCAAAAGTCCCCCAACCCTTCCCTCACTCCC
    CTTCCCCACTCCCACTTGTTGGCCCTGGAGTTCCCCTGTACTGAGGCATATAAAGTTTGCAAGACCAATG
    GGCCTCTCTTTCCACTGATGGCCCACAAGGTCATCTTCTGATACATATGCAGCTAGATACATATACATAC
    ACGAGCTCCAGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTATAGGGTTGCAGATCCCTTCAGCTC
    CTTGGGTACTTTCTCTAGCTCCTCCATTGAGGGCCCTGTGATCCATCCAATAGCTGACTGTGAGCATCCA
    CTTCTGTGTTTGCTAGGCCCCATTATAGCCTTGCAAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTT
    GCTAGTGTGTGCAATGGTGTCAGCATTTGGGGGCTGATTATGGCATGGATCCCCGGATATGGCAGTCTCT
    AGATGGTCCATCCTTTCATCTCAGCTCCAATCTTTGTCTCTGTAACTCTTTCCATGGGTGTTTTGTTCCC
    AATTCTAAGAAGGAGCAAAGTGTCCACACATTTGTATTCATTACTCTTGAGTTTCATGTGTTTCCCTTGC
    ATTTCTATAATAAACCATAAGGAGTCTCTGCTCTACCAAGACCCGCTGCACACTCTGGTCAGTGTTGGGA
    ACTTTTCCCCTATTCCCTCTCTCCTATAACTCCGGGGCTACAGGGTGTCTCCTTTGGGTCCCGGTTGGGA
    GCTGTCTCTTCTCAACCCCCTGACTCATGGGTCAGAGGCCTAAATCTCCACCCAAGGCTGTGTGAAAAGC
    ACCGGGTGGTCTCCCCATATCTCCCTGTCCAGAGCACAGGAACTCTGGCCGGACATGGCATATTTTTCCT
    CCCCAACTTCTTCCCCGGCCTCCTCAGGGCTGTCCCTTCATTTTGTTCCCCACACATCTCCACATGGCTG
    CCACCATCCAATTGGCTGCTGAGGTCACACACTCTGTCTTTGTTCTTCTCTGAGTCTCATGCAGGTTTTG
    TGTTAGTAAATGCAAAAGTGCCTGCTGTGCATGCATGAACCATTACAGGCTGCTAGGCAGAACATGCTGA
    CTTGCTCCTGGATTATAAATCCATCACTGTGATAGCAGGACATATCCAGAAGACATTATTTAACAACACT
    CCTCTTCGTGCTCAACCTCTCATGGTCTTTCTACCTCCTCTTTCTTAGTGTTCTCTGAGCCTACATGAAA
    TGTGAACAAACCTGAAAAAAATCTCTGTGTCAAATACATAGTGTGGATTGGGAAGGAAAAACCCTGCACT
    TGTGTGTGGGCAGGGAAATGCTGGTGCTTTAACAGGACAAGTGCGGTGTGGCTAGAGAAAGTGATGAGAA
    GAAGGGAAGCATGAGAACTGGGTGGGTGGATCCCATGTCTACTGAAAATGCTATGTTGATGTCAAGATAT
    TGTGTCTTGTTACAAAAATTCATTGAGTACCTAGGTTATTCTCAGTATATAAAAGGGGAGGAATGAAACT
    AGAATTAAACACACACACACACACACACACACACACACACAAACACACACACACGAGTAGAGAAAAGAAA
    TAGTCATGAGTTGCCTAGTGAATGAAGAGCGGAAAATGGTGCAGTTAATAGGGATCATAGAAAGAACTGG
    GGGATGCACACGAATGGGTTTCCTGTAATTATGTCTTCATAGTAATCTCTGCTCAATAATCAGACAGTGA
    CAATGTATGCCTCATTTACAAGCCCTGATGGCCTCTCAGAGGAAAGCATCTCTCTGAAATGAATAAAGTT
    CAGAAATGTCCTAAGTGTCCCTGTCACAGGAAAGCAGTGTTGGTAACGTTTCCAGGAAGCTCAGTCTTTG
    TCAAATATCCACAACAAGAAGAAGCCATGTCTAGACAGACAACAGACTGGGAAAGACTGAGCTCATGTAT
    GGAAACACAACTATGTGTCATGCTTTTATTTTCACATTATACTGAGGGGATATGACATTATAGAAACACA
    GTTTGTTCTTTGCATATGCTGAGAGAGGAACTAGATGAAGTGTGCTAATTCTTAGCAATAAAAAGATATA
    CAAAGTGGTACTTTACTGTTTCAATTTTTGTAACTGGCATCATTGTCTTTGATATTTTATATCTTCCTTG
    AGCACTGCCTTCTCTCAAGTGTCCAACTTCAGAGTATGCTATAGCAGGAAGACTACCAAATAAGATTAAT
    TTTTTTGTACCCATGAAAAAATTATGTGCCCTGACCCCTGTTCTCTGAAAGAGGAGCCAAGTTCTGGATT
    CCCAGATCCTCATATTCATTGATCAGCAGTGAACACAGATCATTCACCATGGACATGGGGCTCATCTGGA
    TTCTCCTTATTGTTTTTAAAAAAGGATTTCATTGGGAAAAGCTGCCTCATATTTCTATGACCAGGAGAGG
    AAGATACAGCTAGAGACACAAGCCCCACCATGTAAAATCTGTATGGTACTCTCGTTTTCATTTTACAACA
    TTTTGCTTTTGCATTGCAAAGGAGCAATAAGGGTTCAGCAATCAGCACTGTAACTGTACTTTTAAAATTT
    CCACCTCTACCCTCTGTGTCCTCTATCTAGTGATAGAAAGTGAAATAAGCACATAAAGCATGGGACAAGA
    AGAACACACAGAGGAGCAGAGACTGGCTACAAGTGGCAGCCAGCATGGAGCTGGACAGAATTTAAAAAGA
    TAAAGAGAAATGTCATGGCATGAAGCAGTGACTTTTTCTGTTCACAATGCCTGCAAAAACTTTATGGAGC
    CTACTACTGCTGAGATATGCAGGTTTGGTTCCAGAGAGGATTTTCTGTTTTATTTAATTTGCTTTTAAAT
    TTTTCTTCTTTGTATAGTCTGTATTTTTAAAAAGGGGTTGAATGAATACCTAAGCAGTTGGAAAATTTGT
    GAGTAGAAACCGAGGGCCTGAGAAACAAATAAATCAAAATTTTGAAATGGCAAAAGAAAGGGTTCATTCT
    ATTTTCTCTTTTCAGAAAAGGTTGCAAAGTTGGATGTGCAAATGGAGATGTTATGGGTGGACTTGGAACA
    TCTTGGGAAGGAAGGAACCCAGGAAAAGAAAGAATGAAATGGCTCAGCCTCAATGACTGAGCAAGAGAGT
    TAGAGTCACCCACAGGAGAAAAGAAGAAAATAAACAGCACTGGGGTGAATTTGGGTCAGAGAAAACCTTA
    AAATGCCAGAGGACAATGTGATGAAAGAGACCATTTGAAAGAAAGGGTTAACCTCATTCCATCAGTCATG
    CCAGAGTTAGAGAATGGGCAGTCTGGAGTTACAAGATCACGATTAGCCTTCCCAGTATGTATACAGCATA
    TTCCTGATAATGAGATGAAGCAAAGTTTGACAAGGTAAGATTGTATCCTATAGTAATATATAGATCAAAG
    GCATGTTGCAGTAAATCTCCAACAGAAATAAGCCCGGGCAATGAAAACACAACTCAATTAATATGAATAC
    GTGCTGTGCACCTAGACTGGGCAGATCTACCACTCCACTACCATATGAGAGATCCCTTATAACTTGTGGT
    TTCTCCAGGCCAGCTGCTTCTGCTCTACTTTCCTTCCTCCTCCTCCTATGTCATCCTCTCCCTCACTCTT
    TCTCTCCCAAAACTTTCAGCTGCACCTTCCCCTCTTCATCCCCCAATCAGTGGCTCTAGCCTTTATTTAT
    AAATTAAGGTAGGAAGAAGGTTTATAGGAAATTACCTGAGTGTTGACGTGTTGATAACCCCACACAAGAG
    AACAAAATTAATATCAAATATGATTAGCTCCAGGGTTATATGCAACAAAGGCATGATAAAGTGGTCATGA
    TTTCATATGAGATGTACTGGACTCATGTGAAAAAACGTTTAAATACATGGGCTTACTCAAAATATAGACT
    CATACCCCCAAGATGGGAAGAGACTGGTAACATTTGTACTGGAGGCTGGTCTGCTGTGGCAGCCATTAAC
    ACAGTGGAGGGAAGAGCCTCAAAGAGTGAAATAGAGGAAGGGGAACAAATACAGTAAACCACCAGTTACT
    ACTTAAAGGGCAGTGTTCTGATGTCCTAGGGAGGGCACTCCAAAATACCAATGGCCTTATCCTTAAATTT
    ATTGTTTAATGAGCCTACACTGATCTCAGTGGCCCACTACTAAGGAGAAATGACAGGCACTTGAACAGCT
    GATATGAGAACAGCTGGAGGCACAGTATACAGAAGAGTTTACAAGCCTATGGAACTCCCCTGTATTTTTT
    TATGATGAGGAAATCTGAAAAATGTAAAATGTTGAAAGATTTAAGAGCAGTTATTAGAGTAGATCAACCA
    ATGAGTCTCTTGCAGCCTGGAATTCTTTTATCTTGTTTGTAACAACAATCAGGCTCCATAAATTATAAAC
    TATAAAAATTTACTTTATAAATTATAAATATATATATATGTGTGTCTGTAGATAGATAGATAGATAAATA
    GATAGATAGATAGATAGATAGATAGATAGATATATGGATTTGATGGTTGCAGCAAATTCTAATTGGTTCC
    AACAACCCTACTCACATAATACATTCATACAGGTTTGATAGATGGAGCTAAGTTTTAAATAAATTATTTT
    TCTCCCATGGCTTATATATACACCACCAAAATTCTCAAAAATTAAAATGTGATTGTGTTGTATTTTTCTT
    TTACTGAATGACTATAAAAAGTTACAACATTCTCCATAAATTTACATGAAAAAATATTATGTAGTGCAGG
    TAAAGAAAACAAATTGACCCAAGAATGGTGTATATTCATTACTAAGCAACTTTTTAGATTCACAAAGTGT
    GGGTAAACAAGGTAATTTTTTCAATCAGTTTTTTTTAACTGGCAGGCAGCAATTCAGAGTTACAATGAGA
    AGATTAATAATTTTATTGTGTATTTTAAAATAAATCTTACAAAAATATTAATAGAATCACAAATTTATAC
    CTTTGTATAAAAACAATCAGTCATTTCTACTTTAAGAAACAGAACTCACATCTACTCATCAATCATTTTA
    TTAAGTCATATTACAAAGCTGAGTGCTAACATGGGTATAAGAAAACCATAACCTTATTCACCAGCCCAGC
    GTCAAAAAGAAAAAAACCAGTCATATCAGCTGCTGCTTCAAGAGTTCTTGTTCCTTGACATTAACAAAAT
    CCCTAGCTTAACTATTAAATTTTTTTTCAAAACTTCTAATTGATCCCTTAGATAAATGTTTGTGCTAACC
    ATCGGGACACATCCCATGAGTTCTGAAGCAGTGTGTTGTTCTTCATGCATGGCCCTTTTGTAGAGCTGTG
    AGTGTAGGGGAAGAGGGGGGAGAGAGAGCCCGTGTCCAGCCAGAGATCCTGTGCTCTGGGCAGGCAGACA
    CGGGAGGACAACGGAACACTTTTCACTCGGCCTTTGGTGGGCATCTGGCTGTGTGAAGTTACTGACCCCA
    CATGGTGGGGGATGGACAAGGGGCAGCCCCTGGTACCAGGAGCCCCAGGGCTACACTCTCGGCCCCAGAT
    ATACAAGAAGAGGGCAGAGGGAGAGAGGCTCCCACACAGGCGAGAGTCCTTAGTCTGGTCTGTGGCTGGA
    GCAGGGGAATTCCTTCTGATTGGAGATTAGGCACAGCTGATTAGGGGAAAGCCTACCCCATCATCCAAGC
    ACAATGGACTTTGAGGAACAGAGCCAGTCTAAGCTTTTATAGCTTTATGGTAGAAAGGCAGGGAGAAAGG
    AGAGAATGTGGAGAGAGACAGAGAGAGACTGGCCATGGCCAAGAGGAGGGAAGGGGGAAGAGAGAGAAAC
    AGGAAAAGCTAGAAAGTAAGATAAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAAAGTG
    GGGCCAAGCAGCCCCTTTTCTAGTGAGCTTGTCATCTCACAGTTGCTAGGTAACTGGGGAGGAGTTTAGT
    CTGAAGGTCAGAAGCTTGGGACCTTGTGTATGTGACTACTAACCACAGCTTCTCCTGTCGGGACTGTGGG
    AGCAGTAACTTTGACAGGAGACAGGGATCCAGGAGACATGAGGGAACACCTTCTGTCCCACATAGGTGGA
    ATCGGTTGTACCAGGGTTCAGAACTCAACTCCACTGGAGACCAGCCTATGTGTGCATAGCCCATTGCCCT
    ACAGCCTTTGACATTCTACAGAGTTCTGAGACTGGTAGAGATACAAAATAGCTAGGATTACACAGAGAAA
    CACTGGCTTGACAATAAAACTTCCAAAACAAATATAAATGAATAAATACACAAATTGGAGCAAGTGAACT
    TGCACTAAATCCATCAAAACTTGCACAGGATGGCTGTCTCCATAATGTTCAGATCTACTTCTATTAATGT
    CATTAAGAAGACAAAGATTATGCACAAAAAGAAACAAAACAAGAAACCCAAAAGGTAAAGGCTCCAATTG
    TAAGTAATAGACCCACAGTCTCTGAGGCCTGACTGTGCAGGTCCTCACCTTTGGTAATCAGCACTGAATG
    CAGACCACTCCTCATGGACTTTGGGCTCAGGTTTGTTTTCCTTGTCCTTATTTTAAAAGGTAATTCATAG
    AAATGAGATCCTGCCAGTATTGTGTACATGAGAAATAGAAAAATTGGTTTTCTTTGCTCTATTTTGTTTT
    GTTTGGTTAATGACAGTTTCCAAATCAGCATTCTTTGCTTTGAGGTGCCCAGTGTGAGGTGAAGCTGGTA
    GGGTCAGGGCAGCCTGGAGGGTCCCTGAAACACTCCTGTGCAGCCTCTGTAGTCACTGTGAGTGACTACT
    GAATGACCTGGGTCCTTCAGGCTCTAAAGAAGGGGCTGGAGAGGGTGGAAATAATTTTTAATGGTGGAGG
    TAGCACCTATTATCCAGACACCATGAAGGGCTGATTCACCATCTACAGAGATGATGCCAGAAACACACTT
    TACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
    TGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTATTCATATATATATATATAGTGTGTATGTATATTCATAT
    ATATATGTATATTCATAAGGTTGAGTAAATGGACTTAAACTGATTCCATCACAACTTCCATGGGATGGAT
    ATTTCCCAGTGTTAAGACCTGTCAACATCACTGTCATTCAGGAGACAATGATTATGCACAACAAAACAAG
    AAACCCAAAAGCAGAGGACTCCAATTACAATAGACCCAGACCCACAGTCTCTGAAGATTGACTGTACAGT
    TCAACCCAGCCCTGTACTTCTCTTCCCTAGAATTTACATTACTGAGTAACTGAGGAAAGCTCTACAATAT
    CTGTTCTCTATAGTGGTCAACACCTCCAAACACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTT
    CTTGCCATAATGTAGACTACTTCAGCCTATTTTGTACTCCAGTTAACGAAACTCAAGACTAGCTGCATGT
    TAGTCCTTATTCTGAAATATTATGAACAGGTGACCTCCCATCATTCACCAATGCAATAATCATATTTAGG
    AATATGAGGTTTTATGAGATATAAGCACAAGGGAGAGAAAGTAAGAAAACTACATAGATATATAGACTGA
    CATAAATCAAGACTTGCATGAGCTAGTGCCCAAGTACCATGTCCCTAAGTGGCAAGGAGTATCTTTTGAG
    CCTAGTGAGATGAGGTACAAATCGGACTCTTACATCTTTTTAGATAAACATGTGAGATCAATGGACTAAA
    GGCGTGAGCTGGGCTTCCTTGCAATCCATTTTCCATACAAGATAACAATAGATCTGGCTCCACAGACACG
    ATGAGAATAGTCTTAATTAGTTCTTTAAGTAGAATGACTGATCACTAAGAGCCCAATTCCATACTAAATA
    CTCTTCTGGATTATGCACAGATAAAAATTGCACATAGGGCATGGGGCACTGATCTCCCTGCACTACATGA
    ATGGGGGCTCATTTACTAAATGTTCCCATGTTTCTTCCTAGTGCTGCACAGAGCAAATCCTACAACTTCC
    TGCTTGTCTACAATGTAAACTCCAGACAGTACCAGAAAATCATTCCTTATGTTCCTCTCCAGGTGCTTCA
    ACAAGCACAGTGCAAATTTCTGTCACCCTG
  • Molecule Role : Vaximmutor
  • Additional Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella enterica fliC/guaB mutant vaccine , Salmonella Enteritidis guaBA/clpP mutant vaccine , Salmonella Typhi phoP mutant vaccine , Salmonella Typhimurium asd mutant vaccine , Salmonella Typhimurium asd/rfc mutant vaccine , Salmonella Typhimurium clpP mutant vaccine , Salmonella Typhimurium guaBA/clpP mutant vaccine , Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine , Salmonella Typhimurium hfq mutant vaccine , Salmonella Typhimurium poxA mutant vaccine , Salmonella Typhimurium ruvB mutant vaccine
6. IgG Fc fragment
  • Gene Name : IgG Fc fragment
  • Sequence Strain (Species/Organism) : Gallus gallus
  • NCBI Gene ID : 424904
  • Taxonomy ID : 9031
  • Chromosome No : 9
  • Gene Starting Position : 12628992
  • Gene Ending Position : 12700391
  • Gene Strand (Orientation) : -
  • Protein Name : Fc fragment of IgG binding protein
  • DNA Sequence : Show Sequence
    >gi|358485503:12628992-12700391 Gallus gallus breed Red Jungle fowl, inbred line UCD001 chromosome 9, Gallus_gallus-4.0, whole genome shotgun sequence
    TGCAATTATTTATTTATTATTCTGAAAGTGTTAGAAATATTATTAAAATACAAGAGCAGAAAGTAATACG
    TATGAATTGGGGGGAAAAAAACAACACATACAGACACAGAAAAACACACTAACTACAGGTATGTACTTTG
    GGGTTCATCTTGGTCAAATGCAGCGTTCACTACGTAGCCATAATTGTGTTCCCGTACTCTGTCACCTGAT
    GAATCCTCAGACTCGTGCAAAGATGAATTCCGTGCATTGATGTTGGCATCTGCCTTTCTCCTCCTTCTGT
    ACACAAACAAACAAACAAACACCAACAACCATCTTTAATTTCTTATCTGGTCAAACGGTGGTGTGAGTGT
    GTGTGTCAGATTCCAAAGAATTCTAAGTTTTGTGGTAGTGGTGCATATGCATTATGTAATAGGAATGGCA
    CATCTTCATTCCTGTAGAAGAAAGCTTTCCCTCGTGTTCCTTAAATGATATCTTGAACGCTGTACTTCCT
    TTCTGATTCTGCAGGTGTGGGAATAGAGGAGTTTGGGGGTGTACAGAAGCCAAAATGAGTGTCTTGCCCT
    TGCTGAATGTAGCAAAGCTGGGAATAGTCTTACCTGAACAGATACACTGCAGAGGTGATGAGAATGACAA
    CGGCAACCACTGCTACTGTAACTCCAACAGCGATGGCAACTGTATTGCTCTGAGAACTCTCTGGGATACT
    TGTGTCATCTGAAGGAGAAAGGAGCCCGGTCACTATAAGCTAAGAGCACTGAGAACAGAAGATTCTCCTC
    AATTCCAAGTGTGAAACTGCACTGTGTGGAATCTGAACAGGAAGCAGACGTGCCTGTCTGCTTGGTTTAT
    ACTGTGTTGTCTGACTTAACTGGCTTTCTGGAATGGTGTCAGACCAAGGTCCTGTATGCCCTGCAGAGCC
    CTGCTGTCTTGCCTTCTAATACCTCTGTGCTGAGGACCTGCCCCCTTCTTTCTGTTTGAACATACCAGTA
    CCAACATGACCACCCAGCTTGTGGTACAAGGCCCAGTTTTTACTTGAAGGAATAGTGCCACTGAATTGGT
    TGGAAAGTGTAGTGGAATATTTCTAGTGTCAGCCTCCACTCTGCCCATGACTAAAATACAATCTGCCCTT
    GAAAAGATGTGATGAGGTCCCAGACTGATCTGACACCCCCTCCCAGTGGGTTACTTATTGATTCACAGCA
    TGGCAATTAAAAGCCAGCTCAGTCTTCTCCATAATGACACATTTTTTTTTTTTTATAGTGGTGTGTGTAA
    TTCAGGCAGAGAAATCCAGAGTAGTTTTATTACCTATCCTTTCAATATTGCATCTTTGTCCCATGTACAG
    CTCTGTACATTCACAGTAGTAGTTATCAGAGGTGTCATTGGTTGTTTCAGAGCAAATTCCATCATTCTGG
    CAAGGACTTGGCATACACGGTCCACCTAACAAAAAGGAAGGAGAAACACATCAGAATCTCATGTAATCCA
    GCCCTGAGAGAACTACTCTTGGTTAGATTACACTGCTACTGCTGGACGTGCCCTGTGTTTTAAGGCCTGT
    TATTTATTTTGTACTTGATAACAGGAGTATGGGAAAGAAACAAAGTTCTCCAGTAAGCTTATAAAGAAAG
    CTTATTATTTCTTCTGTTCAGACTGTTGCAACCCCAGACCCAGACACATCCTATAAGCAGTAACTCAAGA
    CTTCTTAGCAGCGAAGGAAAACACGATAGGTTTAGTGGTTCACTTACTCCTGTAGCACCCACGAGTGTAG
    TTTGCAATGTTACAGATTTCATCAGAACCACACAGTATGTTAGAGCAGGTGACAGTGGAGCTCTCTGTGC
    ACTGGCATTTCTGGGAGCAGTCATCAGTGGTGAACACCTCTCCAATCTGGAAGATAGCAAGTGAATGTTA
    AAAGATATCCAAATCAGGCAGGGAAACGTGAATGGAACACTGGAGCCCCATGCTACTCCGATTCTTCCTG
    ATGAATGACAACATGGCAGATCTCTCTTTTTTGTACTTTGAATACAGAAAGCAAGCAAGCAGCTCATTAA
    CCCATCTCCTTGAAACTCAAGGAATTTTGATGAAAATTTGAGGTGTGAGATCCCAGACCCAGGTTGGGTA
    TATGGACAACCAACAATATCTAACTGTTGCAGGAGATAAAGTGGCAAATGCATCATATTGTGCCTCCAAG
    ACGATGTGCAAAGGTGCCATGTGTCATTTCTCTGGCAAGCCCCATTTGTTTAGAGTGTCTACCTCCTCCC
    ATAACACCACCTTTACTTGCTTTAGGCCTTCTCTGTATCAAACCTCCAGTGCTGAATTGACTACAAAGCT
    TTTTATCCCACAGCTGGAAGGGTGGAATCTCTGGATTCCTCAGAATCCAGCCTTGCCGGAGGCTCTCTTC
    ATGCTGCAGCCAGCATTACAGACTCTCAGCCCCTCCTGTGCCACTCTTCACTGAGAGGAGAGGGACCATG
    GTGACCAGTAGAATTTTTCTATAACTGGCGTATTGTAAATTTTAGAGCCTATTGTGGCTGTTTACTGTAA
    TAAGGGTGAAGAATTAACAAGGAGGTCAGAGTGTAATCATATGCTTCCCCCAAGTGCCTGCAGAGTATTC
    TGACAGGCAGAGTTTCAGAGGCAGACTGACTCATGTATTCTCTCCTCCCTTCCTCCAGAGACCAGAATAT
    TATTAAATAAATTGCTCTTTGTTTCTAATGCAAAGTTACATGCTGAGGAGAACAAAGAGATTATCAAAAT
    AATTAGCACAAGAAAATATTGTTTAGCACTGCGGGAGCTGTCTGGCCTGCTGAGACACTGTACCTCATAA
    TATTTGTCAAGGTATGTGCAGCCACATTCTCTGTAAGGCACACATTCAGAGTCACTAAGAACATAGCCAG
    GGAGACATTCACAGCCTTCAACACAAGGCGATTCACACTCAGAGGGGCTGGTCATGTCGCTGCAGGATGC
    TGGGCAAGCAGTCATGCATGAGTTGTATTTGCTGTTGGGTGGACACGACATCTCTGTAAGAATAAGGAGA
    GTCCAGTTATAACAGGTTGCTGCTACACATTGCCTTTTCAGAGCTGTTTTGTAAGGGCAAAATGATGAAC
    ACACATACATAGAAATGTTCACATGTATATAATGGTAATGAAGTTATAAGTGAATAACTTGAAAATAAGG
    AAATGCATTCTCAAAGCTATGCATATGCAGTGCTCTGAGTCCAGGTGTTCACTAATATCAAATTACTTCT
    CACACATACAGAGTAGCTATCACTGAAAGCATCAGAGTAGTTTCACAGCTCTGAGAAGTGCGTTTCATCA
    GAAATACAAAATCCTCTGCCCATGGGTGATGCAGGGTAAGTATGGCCTGGATAAACCTCTCAGCTTGGGA
    AAGAAAAGTTAGGAAAAAGAGGTTTGAGATTATTGTAAATCCTATTTTCACATTTTTCAGTCTGAAAGAC
    GAGTTGGGAATGGAGACATTTTATTTTTCTCACCGAACTTCTCATGGCAGGGGAGCTGACCGCTCTGCTC
    CCCTGAGCAGCTTTCTAAGGTGGAGTCTCATACAAAACATCAGCATAAGACTCAGAGGCAGTGACTGAAG
    AGCAAGTGTGCGTGCTAAACTATGAACGATGATTGAAGTGTGAATCAAAGTTTGTAAGACCTAAGAAGCT
    TTGACATACAGCTTCTAGCAAAAACCTCTAATCCCATATTTTGAGAGTCCTTTAGGCAGAAACCACACTT
    TCAATTTCTTCCTGTACTTACCACAAGCTGTCTGTTGCCTCCAGCCTTCCATATTGACTCCTTGCTCCTG
    ACAAGCCAGCACGTACTCCTTCAGATGGCGACATAACGTGTCAGCATCATCTTCTACACACATATCGAAG
    ATGCAGTTCCTATGTACAACATGAGAAAGCATTCATGCACTATACCTTTTATTTTTTGTTTGCTGCTGCT
    ACCTTCTCAACTTTGCCGATCATCTGAATAGCAGTGGCCTGATTTTCAGAGGCAGTGAGAACTGAGGTGA
    ATGGGATCTGCAAGTATACAGTAACTCAGAAAAATACAGCAGCCACTAGGAAAGTAAAACAGGCTGCCCA
    ACGAAATTCTAGAGTAAGGAGAAAAGTGGGCTTACATTAAGAATCAGGATATAGACCCTGAAGTCAGTGG
    TCCATTTGTTTGGTTTAAGGTACTTCCACATTCCTCTCGCAGCTAAGAGAGGCTTTGAAGTGAGCACCAC
    CTATCTTTGTGCATTACTTACAACTCTGAGAGTAAAGAAATCTGGTAGCTGCTTGGCCATTCTTTGACTG
    ATAATGAATGAATGATAAACCTAAGAATTTATCAACAGGTTTCATATGGTTCAAAACCAGGTGTGTATGA
    ACTGTGTACTTAGAAGGCCAGATGAACAGAAGGAACTTTCTTGCCAGTGCCATGTGAGTTATCCTTCACT
    AAACTGGTTTTTTGCACCGCTCTGTGAAAGACTGAAGAACTGTCACTGACAGCTTTAGTCTCTGCAGCTC
    TGACTTGAATACTGCATTTTACAAAACTACAATTGCTAAGAAGAACCTAATAGAGGCAGGTACTGACTGA
    ATTATCACTGAATGCGAATGAGAACTTTGAGCACAGTGGAAATTCCAGCCTACAGATATAATGTTCAATG
    CTGCTGCTTTAGGTAAAGAGGAAAGCTACTTTCTAGAGATAACAGAAAAGTGAAACTAAATAGAGAGAAA
    AACATATCTTGTTTTAAAAAACAAACAAACAAATATTGGGAACTAAACATATAACTAAGAGAAGCTCTGG
    GCCCAGTTCTCCAAAGACACTTACTGTCCTCTGCTCCTAGAAACTGAAGACGTACAGTTCTTACCAGAGA
    CAAGCTCCAAAATTGTTTTGGAAGTGCGGAGAAAAATTGCAGATTGCAGCAGACACTTACGTGGAATAGG
    GCTCTGGTGGGACTGCAGAATGACAATTTGCAAAAGGACCATCCAATGCAGTCAGAATTTGACACTTAGA
    AGTGCTGTTTTCTTGCATCAGCTTATTTTCACTGCAGCCTTGATAGAGCCCTGTATCTGGTTCCTCATCA
    GGCTCATCTTCCACAATGGTTTCTCGCCTGGAAATGAAATCATTATGAAACCACCAGATCTCTGTACATC
    CAGCACTCATAGCACTAATTTGCAAGTAACAGCATTTCTGCCTTTTGATAGAAGTGTCAGCTTATTTCTC
    TCTCAATATTTATACTGAATGTGAAATGAGTATATAAAGCAAATCAAAGATGTACTAGAATAATTCCTAC
    CATTTCAGAGATATATGAGTTTTTAAATAAAAAACAATAGATGTTGTTATCTCCATGGACATTCTATATG
    ACATAGGTTTCATCCAGGCCTCCTTCACCTAATCCATTCTATAATAATTGAATGCTAGGCAGCTGGAGTA
    GACCACCATTTATTGGAAGTATGTTCACTTTCACTGAAGAGCATGCCTGAAGTGTCAGAATTCTTCTGCA
    GGTATAGATTCCAGAGCCTCCCTATGCTCAACATTAGCCTACATGCTGCTTTCTACTATCCTGCTGGACA
    GGTTTTTGCAGCCAGATTATATCCTGAAATGAATACAATTCTATTCATGTACTGGAAAAAAACAAATAAT
    ACAGTAATTTCTGTTGGTGGTTAAGCTACTCAAAAACCTTTCAAAGCAAATTGAAAAGGTGGAAGGAGAG
    AAGAAAACTTAGTTCCTTGCTTCAGTGTTCTGTATGGACTAAATAGGGCAGTTTGGCCTGATCACAAATA
    TCACACTGATTTTTATTCAAAAGAATGTCCCAGGTTTTTTTTTTTTTTTTTTTTTTTGTACTAGCAAAAA
    ACACAATGTAGTCATGAAAGTAAGGAAGTTAGACACTTGAAAAATCTCTTCTGTGAGGAACTGTGAATTC
    ATCCTGATGGATTTTGAAGGTGCCTTCATGTTCCAAAGAGAAGGATGACACTGAATCAGGATCTGCCTGT
    CCATCCAGCAAATGATGCATTTAATCTTTGCCAGGACCCATGGGCTTGGCAGATACCTTATTTATCCCTG
    TGCTCTAGCTGTTGTAATGGCTGTGACTCCAAAGGAAGCTGAACTAGAGCAATGCACTGAGAGCTCAGTC
    CTGTTTTGGCAAGATGGAAAAGCACTGATATTTCACCTTAGTGTTGAGCAGGAAAAGTCCCCACATGCAG
    ACTAAGGGAAGAAGTAACAGAAACCTGACTGCATAGTTAGACCTGAATATTAACTATGGCTTAATTTCCT
    CACTTCCAGTTGCTGTGTTTGCTAGGGATATTAAGTAGAAAATTAGGTACCTGAATCGAGAGGTTCTTCT
    TTTCACAGGAACCTTCCAGCTCTCACCAAATGCATTCACATTCTTCAGCCAAACACCATCTGGGTTTGTG
    AAGTCATTTCTAGGTCTCCCGTCAAAGTCACCACACAAACCTTCTACTCTGTTGTTGTAGGTGTTGGCCA
    GCTTTATATCTAGGAAAGTTCAAAGGAAAAAAAATAGCCATTACAAAGAAATATTTTCTTCCGAGTATAT
    GGAGGAGGTGGAAAGAAGTGTGAAGTACTGAATTCCACTATTCAGACTACAGTTGGTAGTGTGATGAAGG
    ATTTGAAATCATCAGAAATACCATGGCTGCATTACATTGTAAATCAGGGGTAGCTCCACTTTGGATAGAG
    GAGGTAATGTGTTTTCAGCTTACATGAATGAGTTCTGTATGCATGATATGAAAGTCCTGATGAGAAACAC
    TAAAGAATGGGATTATCTGGAACACGCTTGAGAGGAAGCATGTAAAAGAATGCACTCTCAGTTGTTCAGC
    TTGAGGACCTCAGTCCTGTAACTGGGTTAAGCTGGGAAAGGGGCAGATCCAGACTTTTCCCCATCGTCCT
    GATCAGCAGCTTAAAGTAATTTGCTTTTGTTTCCATCACTCAGGCCTTACCTAACATTAAAAGTAGTAGA
    ACAGTCTGACTCCACATACAATTTCTCCTAAATTCAGATGGATCCAGATAGAGAAATGCCAACTTCCATG
    AGAAATAATTTTTGTTTCAGTTTTCTGGAAGTTCATGTCACAAAGAACCACAGACTTAAAGGGTGAAAAT
    TTCTAATTTTATCTTGGGTTTAGACCTACTTTGTAATGATAACATTTTTTGACATGGTGCATAAATATTC
    CTGCTGATTGTTTTAGTATCACTTACCTGCATTTTGATCACCATCGTAGCTCAGGTATAAGCCAAAATCT
    GTCTCCAGGTAAATTCTTGTTGTCCTCTGATATATACGAATACCATCATGGGGACGAACAGGGGCTCTCA
    CCCTTACACCATCCAACTAAAAGGGAAAGAATAAGTTAATACTTATAATACAAATTCGTATGTGGCTTTG
    ATGCAGGTAAATGCTACAAAAGAGCATTCCTCTTTTCTGGTTTTATGATAAATATATAAGATATTTTAAC
    CAGGTAAAAAATACAGTATTCCACTTTTAAAGTAACAAAATCATATATTAAGGAAAGTGATCTGGACACA
    GTGTTCTTACATCATTATCATTCCTGAGAGATTTCCAGTACTGAACAAAAATGCATGTATGAGCCCAAGT
    ATGCTATTATGGACCAACAAAGAGGACAGAAACTGAGTTTTGCCAGCATAATTCACACTTGTCCCTACCA
    CTGATCATATTTTATGTGGCTTTTTGGTAAGGTTTGGATATTTGGTATTTTATTCCTAGAAGTCCATTTA
    TTTATTCATTCTAACTAGAATCTATCTTTCACAGCTTTAGAAAAAAAGAACACAACTGAAATCACACTTT
    TGCTTGCCCCACAGTACTGCTGTAATAACACTGTTAGCTTTAATACTCTTTAGAACAATCCTTTAATTTA
    TCTGTTTTACTACAAAGGAGAACCTTTGTCTGTGCTCTGCAGTTAGTGAGAGACTTACCAGTATCTGCTT
    GCCTTGCCTGAATCGCACTTCGTGATTGTAAACGTTGATGATAATCTCTTTCAAGTAAGTAATGCGTCGG
    TTTAGACGAAGAGGATAGTTCACGCCTTCGACGCTGAACTGTGTCAGAGTATCAGGTAGGTCAGGGATGG
    TCTGAGTCAGAATATACGTGTATTTCCCTTGGAAGTGGTGCACCAAACCATCAAATGTGATATAATGTGG
    ATCGCCTATGATCCGGCACTTGCCAAAACCTAAAGAAAAAGAGTTTAATTGCCTTCCAACCTGAATGCAC
    GTACCAAATCTTATGCATAAATCTGCTTAATACAGTTTAGATATAAATAATTGCTGCTAACATGCAATCC
    TCTCAAAACAAAGACGGGTGCTCAGGCATCACCCTAGCAAATGGGTACTCAGACAGAAAGCCAACCAGAG
    AAACTTATTCTTCTGCTGAGAACCAGTGGGAACCAATAGAACTGAAGCACGGCATTAGGGTTTATTGCTT
    TTATAGGCACTAAGCTGCACACATCAGTCCTCTGCAGAGGAATGACTGCCACACAGGACGGCTTGCAGTG
    GGCTTGTAAAGATGTTGGTTTCATAATCCTCAGCAATATGATCATCTGATCATCAATACAGTAGGACCTA
    TGCCATCCTATTCTTGAAAATTCATGTGTAGAGGTGTTAGGCAACAAGCTACCAATTAAACTGTCCAATT
    TACAATGAGAACATGGGATTAACCATAACATAGCAGGGCTGACACCTCAATGTAAAAGCTTAGCCAAAGC
    AAGTACTGGATGAAAAGCACCAAGATTGGCTATTAAGTATCTGTTGTGATCTGCTCTGTCCTGTTTTGCC
    TTTATTTTTCATATTTTCAAATCAATATGCAGTGAAGAAAATAAAAAAGAATGCCAAAGGATTAATAGAT
    GTATATATTAGACTTTTATTTTTAGTAAATGCAGTGGATATATATATATATAATCTGCCAAGTTTCTAGA
    TGTACATAGATTTTCAAACTCTTGTTCTAATTCTCTCTACAAATTCCCTGTCTTGTACTGTAACTTTCTA
    TTCTGATAAATGAGGCCTTTTTTTGTACTTAATAATGTTTCATCAGTAGTCCTGTCAGACCTGTCTTTTC
    ATGTCGTAAGGGTTGGAAGTGACATGGAATAAAGAATGGACATTCACATCAATCCTCCTTCTCTAGTCTT
    CATGGAATAGCAGATGAAGATCTGCCTGCACATACCTGTTGGATTGCACTTGTATTTTCCACTTTTGATC
    AAGCAGGTTTCTTTAGAATCACATCCGTAACTCTGACATCTGATGACACCATTCTGCTGACATTGACATC
    TTTCGGTGCAGTGTGGAGTAATCCAGGTCTCCCCAGCCTGGACACAAATTGTGGTATGTTACCAAGGTGC
    TGAGCAATAGAAAGTCAGTAGCAAAACGAGTGGAGTCTGGCTAGGTGCATCCTGCTGGCCATTCCACAAC
    TGCTCAATCCAGGTGTAAACTTATCAAGAAGCTGGGGGGGGGGGGGGGCAATCTCAGTCCATTTATCTTC
    CACAAGGGAATTGGAACACATAACAGGAATAGGATATACCACCCAATTAACGCGGTCAACTTTCCAGTAT
    GTCTCAATTTTGGCTTCAAGGTGCAAACGACCACATTCGTGCATCCTCTACCAACTTAAGCCCATAGCTT
    TTGCTAAGCAAATGAAATGTATTTAAAATGGTAGACCCTGAAGACTTTTCTTTCTCATTCTGTTGGACAC
    AGAACCAATTTCCTGCAAGTTTTGCAGTCAGAAATTTACCCTACAATGGACTACTTACACTGTAGTAATT
    GTTGTCATCATCCCTGCAGCCACAGTTGCTGAGAGGAACACACTTGTCATTACTGAGCACATAATTATCA
    TCACACTCACAGCCCTCTGTGCATGATTCTGTCTTCTCACAAAGGGATGAGGCAAAAATGTCATTGCATG
    TTGATGGGCAGGCAGAGGCACAGGCTGTGTAATGGCTGTGGGATGGACAGGGCAATGCTGTGTGGGTGAA
    GAGAATAGAAGTAGTGTTATTAAACACGTTAAAACAAATGTTCTTGCCTGATATCTTCAACTTGGCCTGT
    AAAATAGTGGTGTAGTTCTGCCTTCTTATAGATCATTTTGATCATCAAACAAAAACTTTTTCTTCTTTTC
    TTTCTTTTTTTCATAATTAGATGGTAGCTTTGTTGAATGGATGGAGTGTGGCACATACAATGGAAATTAA
    TCCAAGAACTTGGCCCCTAATAAGCAACTGTCTGAATTGTCAGCAAGTGGCTTTGATCCCTGTGTTCAGT
    TCAGTTTCCCATTTGGTTTTTTACTTCTGGTTATACTACGGTTATTATAGTTGGACTTGATGATCTTGAA
    GATCTATTCCAACATAAATGATGCTGTGATTCTATTATTTCTCCATTTTAGACTCACTGCAGTATCTCAA
    GACATTCATTTGGCTACCAAAACCATTCTAGCTCCACACTATCACCCACAACTCATCTAGTTGTTATCAC
    TGGGTTTAGTTCAGCTTGCAAACAGTTCAGCAACATAGTCAAGTCATAAAATCATAACAACAGAATTGTT
    TATTTTGAATATTTTGTCTTAATTATCTTTTATATAACTTGTCTACCTAATACTGCTCATCTGTCTTATG
    CTGTCAGCCATCCAATTTTCTGTTTTTTTTCACTTTCTTTTGCACTTACGACAAAATGTGCTATTCCTCC
    ACGTGATGGTGATGCCTTGATTCCTGCAGGTGTCCACATAGAACTGAACTATGTCACAGAGGGCAGACTT
    CATGCCATCATATCGACACATGTCATAGGTGCAGATCTTTATGAAGTCTTCGGGATTCACCAGATCATGA
    CATGCTTTGAATTTGTCTGATTTTAGGACGTTGCACTGACTTTCAATGATTGGTTTATTCTCATCAGTGC
    AAGGAGGAACATCATCTTCTCTTGAGTCTGACAAACAACTGCAAGAAAGCACAGAAACAGCTGCACTTGA
    GTTTCAAAGGCAAATGTGAGACTGAGACAGAAACTGGAATGTTTGTTGCCACTGAAAGGTACTAAATAAA
    TAGTGCTGGAGACTGCAGTCTTACTTCTGTCTATGGCTGGAAGAGTAGCAGCAGTAGATGTGCATGACAA
    TCACTATGCTGTTTCTTGACCTTGGTTATGCTCACTGAACCTGACCCTGACAAGAGGACTGATTTCCCAT
    CTTGACCTTGGACCTGCCTCATCTCTGCGAAGTTGTGTGGTGACCTGGACTCTTTGCTGAGTCTGGCTGC
    CACCCTCGGGCACACACACTGCTTGCCTTCTGTGGACACTCTGCATGGGCTTTGGCTGGTGAACCCATGG
    TTGTACAAGCTGTGTTACCATGCTAGGCTCTCTGTACCTTAGGGAACAGCTGGCCATCACTGTTCCTGAA
    CTCCATTCTCCTGTTCTAAGTATGTTTTTCAGTGTAAAATAAATGTAAGCTGTGTCCACACTGTGGACTA
    CTCCAAAGCCTAATTCCCCACGTGTGTGTGTCCACAGTAAAATGGCTATGGTTTCCATCTGGCCTGTATG
    GTGATTTCTGGTGGTGATGGCTTTTTTTCTTTTGAGCTTTTAACTGAAGAAGACTAAAATCTCATACATA
    CTGTACATTACAAAGTCATAAAGTAGACAAGGCAATTAGAGGAAGACCGTTCCAAAGAAAGATGGAAGGT
    ATGAAGTAATGCTTACCCTGCATCACTGTCGTCTTCTACTTTCCAGCTATTTCCAAACTGTGTGACACTG
    ACAAGTGTACCATTGGGCAAGGACAGATCATCTTTATAATTGTCGTTGAAGTTACCACACATGCCTTGTA
    CCTGCAAGCAAACAAGAACTATACTCTAAAAACAATCTGCTCTGCACCAGCTTCCCCAGTAAGTTCTTAA
    TCATTGCCTCTGGAATGATAAGGTTGAAACACTAATGACAATCAATCTTATACTAAATATTTCAGACATA
    CTGATTGCACAGTAGAAAATAAATAGACACTATGATACCCTAAGACTTCAAACCATGTAATTCTATAAGC
    TGGATGTATTCCCCTAGCTGTGTCATTTTCTGTCAGTTCTCATTTGGAACACAAGGAAATTGGTGCGAAG
    AGAAGAAATAGGGAAAAAATGACTTGTGTTACAATACAGCTGTGAAGAAAGATGCAATAGCAAAATACAA
    GAACAAAGAAAATGAAAATAAATACACAAGCAGTGGTTTTGAAAGAATACCAATATGTCTACAAATTAAA
    TCTTACCTTTGAGAAGTAAGATTGTGGGAGAGTTATTTCCAGGTGGTCATCGCCATCATATTTGACTATC
    ACACCAAAGTCTGTTTCCACTACTAGGTAATTGCCAATATTTCCAATGGACACCCCCCGCACCCGGTTCT
    CCACTGGAGTGTAGACCCTCTCACTGTTCAGCTGGAAACAAAAATCATTTGTCAGTATGGATGATCTCCC
    ATGAAAGCTTTTCTGCTTGAATCTACATTCCTTGGAGTGCTGAAGATTACTCTGACAGTTTTAGATTCAT
    CACACTTTCACTATTACATATTCCACCTACAGGTTGCAAAAATCAGCTTTTCAGCTGAGGTGAGCTGGGC
    AGGGCTCTGGTGATTATAGCTATAGGTGACTGGCCTGACAACAGTTACTGAAGTGGGGAGGTACATTTTT
    TAGTTCAGAAAACAGTTCTCAGATCTGGAGGCTCTGGGCTCCACCTCCAAGAAATAGCAAATAGAACAAT
    GAAACGAGAAGACAAGCCTTGGGAAATAGTCAGGAATTATATTTCCCCACAACATCAACTTAAGGGAGGT
    GCCTGTAATTATTAAGGATATGCATACACGTTTCTTATGAAACATAGATGTCCTTTTTCCTACAGCTACT
    GTAGTGAGCTAATTTTAAGATAATTCTTGCTGGAATAAATAGCGCCAACACCTACCAGGATTTCTTTGCT
    TTTCCGAAGGGTGATTCGTGTACCATACACATCAATGTAGACTTCTTTCAGGTATGTGGCCCCTCTTAGC
    CCTCGGTCTTCGTTCTTGCCAAGGATGGTTATTGGGTTGACATTGTAGCTGACAGACTTAACCAAAGTGT
    AGGTGCAGGTACCCACAAATGTGTACATCAATCTATCAAAGGTGAAATAGTGGGGATCTCCTGCTACCTG
    ACACGTTACTTGGCCTGAAAGTGAAAAAAAGAAAGATCTAAGGACTTTGGCCTGGATTTTACCATGGACT
    CAACTGACCATAGACATTTGTATTAGTGCAGCAACTTAATCCTCATCACTATTACAAGGAGAAACTTTCA
    TAGAAGTATTTCAGGTTCATGTCAATGGCATTTACCTGGTAATGAGAAATTTCCAGTAGGTATCTCCTAA
    ATTTAATTAAAAATTACATACAAACTGGCAACTTGGCAACAAATTAACCTGTTTATCTGCTTCTATATGC
    TGCTGTATCCATAATGCATGCTAATTAAGAGTAATCCAGAGCTTTCCTATGTAGCAATTTGAGAATACCT
    AAATATATGAGTAAATATAGGTATCAATATGAATGTAAATAAATATGTATCTATATACATAATTATGTAA
    GAAACTTGCCAACAAACACTCTCTCAGAAATGGCTCTCAGTCCCAAATTTAGAACTTGCAGCACTGGTGA
    TTTGTTTCTTGAATTTGTATGATCAAGACAAAAAGAATCCAAGGGACCTTACAGGCTCTTAGGCAGCATC
    AATTTTTGCAGATATCAATTACAGAAGAAGGGAATCAGTGACCACAAGATAGATCATCATGTCTTCTTGT
    ATCTTACCACTGGCAGCAGTTAGTTGGAATTATCATTGCCATCACATATGAGAATGTATTTATGAGAAAG
    TTAGAGAGAGAATAAGAATCTAATCATAGAATTGACACTGAGAAGAATTTTCTGGGAGGACTTACCACTG
    GAATGACAGCCCAGCACACCATCCTTAACACTGCATTCCTCTCGTACTCCACACTCCCAGGATGAACAAG
    CAATCATGTTATTAGCATTGCAAGTGCAGCGTTCAGTGCAGGAATAATTGGTGAACCAGCTTTCACCCAG
    CTGAGGGGAAAAATATGTAGAATTATATACTATAAGATAAATAGGATAGTAATGCCATAAGGGACAATCA
    AATTACTAGTATTTGAATGTGCACAATATGTGATCATTTACTCACTTATGACAACAGAACTCAGTGATCA
    AGAAGGCTGGAAAAGTGAATCTTTTTTGCTATTACTAACAGTGTAAATAGCAATTGTGATAGTGTTTTTT
    CTCACAATGATAGCAATTAAGACATGTTGTTCGTTCTTTTTTTTCTTTTTCTTTAAATATTGTCTATGGT
    AACTTGCCTCATGATAGTGGTTTTTGTCATCAACACAACCACAGTTGCTTTCTGGCACACATGTGTCTCC
    ACTCAGAACATAGCCATCCTTACAGAAGCAACCCTCCACTGGCAATGAACTACAGGTACTAGCAGCCGAG
    GAGTTCACACAAGTAGAGGGACACCTAGTACCACAGCCTTTGTAGATGCTGCCTCCAGGACAGGATATTG
    CTGGAGGAAAAAGGAAAAAATAAGATCACTAAAAATGACAACATTTTTATAAAAATTATACCGATGCTTT
    CATTTCTACTGTTCGCTTCTGAAAATTAGGAAAACTGTTGTTGGAGAGTCTGACACTGGAGACTACAGAT
    TACCAAGAGTCTTCTTGAACAGTGAGATGGTGGGTGGCATCTGGCAGTGGTGGGCAAGACCAGTAGGCCA
    CCCTGCACCTATCTCACATAGGAAGTCAAACATTGCAGTCTTGCCTTGAGTGTAGGGCTGACATGATCAT
    ACAGACCTAAATGTGCTTCATTCTGTAATCCTTCTGGAGGTTTAAAGACATCCAGATGTGCTGGAAACTC
    TAATCCTTCTGGTTTTGGCTGCCTTTAGTATCTAAACAGGATTACAGCAGCAGTGCAGAGCTGTCTGGTA
    GTTTTTCTGAAAACAGCTTAGCTATGTTCAAAGCCCTGAAGATCACTAGTAGGTGACAGAGGGCAGTAGG
    GAGGCCATGGAAAAGAGGAGCATGGAAAAGATAAAACCCCTAAGTATGTACAATGCAGCTATGTCATGAC
    AGATAAAAGTTGCTGAGGCACTGCTTTCAAGTTTCATCAGAGTCAGAACCATGTATGTTCTGTCTTCCCA
    CCTTTGGGCAATTTAAGACAGAAAAGAGAGAATGGGGAGGAGCTGGTTAGAAAGCTGGGGCTGGCCAGGG
    AAGATATAAGGCTGCAGGAGGGATTACATCTTTACAAAGCCAGTTTGCATTACATGGATCTAAACTTCAT
    TTTCAGCAGTATAGTGGACAATAGTAAGTTTCATAAAGGTTTAGTTTGAACCACAACTCTCAAGGCTACT
    CATATCCAGGGATTAAATAAAGCATTTCACTCCTCTGAGCTCTGTTATTTCCTGACTACTCAAGTCACTC
    AATTAAGGGAGTAGAAGCATCTCTTCACATATCAGATGGTATGTCTCTGTTACTGCAGTTACTATCTGAA
    CACCGGTCACATTTAAAAATGGACCTAAAAATCAGATTCTTTTCCATTGAGACCCCCTGAGAAAATACAT
    TGTGCTCTCACACCCCTCAGGGAAAATACTGTATTTTACAAAAGTCTTGAATCATTTGTCTATTCCTGTG
    GTTTGAAAAAAACACCAAATAGTATTTTTGTGACGATCCGTGAAAAATCACCACATGTGAGTCTCATTCA
    CTTCCATAACTACAGCAGGATCTCTTACTCCTGTCAGACAATTACAGCCAACACCACCAGTCATCTATCC
    TTTTCAGATTCCTAATTGATTTTTTATTTGAACAACTGATGGAAGGAGCATTTGAGAGACAGTCCTACTA
    ATGTGACTGAGCAATCATCTTCTCTAACACCATAACTAAGCTGTAGGTATTTAGGTCCTGGAATATGGCC
    TTAGACTGTAGAGCCTGGAAGTCCGTACTTTTAATCTGAGTTGGGGGCCTCAAAGCTATTTTCTTCTTCT
    GTCTTCTCTTTACAGACAAAAGGCCAGTGCTCTGCCCTAGGGCTGACTTATGTGTGCTTTTAATCAAAGG
    CCATTCTAAAGTCATTCTTTTACCAAGCAGCTCCTGTCATAAAATTATTCCAAACATAATTAGTATGGAG
    ATAAATGAGAATACTTCTGTAAACTTCCTAGAAACACTTGTTTCTTAAAATGTCTTACTAAACTAATTTT
    TCTCCAAAGGCCATCAATGAATATTGAGAGCCTACAAATATCAGCTATGTTCGTATTGCGAATGGTGGAT
    TTTCAGCAAAGCAACGTAATAGTTACTCTCCCAGTGCAGAAGCTCACAAGAGATACAACTGACTATAATC
    CAAACTTTCCAATAAAATAATAGGAAATTATAAATATTTGAAGAATTAGACTAAAATGTATCCAAAAGTT
    CATGAAAATTATTGAATTATGACTCCCGACATACCAAAAGACTAAACTCGAAGCACTACATATATTGAAA
    CATCATACATGAAAAAGATAGAGGAGAATGTGAATAATTTAAAGTTTAAATACAGAATAAATATTTATAA
    ACCACAGCACTCACGGCAAAGAGTAGCATTTCTCCACTCTATACATATTCCAGCATTGATACATGACTCG
    GCGTAGGCCTGCAGTCCATAGCATAAGGATGTTGCCTGTCCCTCAGTGAAACACATGTCATAAACACAAT
    TATTGAAGAAATTTTCGGGAGGCACTTTGTTGTGGCAATCCTTGAAGATTCCTGTGTAAACAAAGAAAGA
    TACATAGAGGACAGAAAAGAAATGGGGAATTTATCACCATCAGCATCTATTGTCTGTCATGGCAAATGTA
    TTGAGTGCTCCAGGCTGACTTTGTTTAGTAAGTTAAATCAACACTTGAAAATGAGCACATACAATAAAAC
    CTGGCCCTCTGGATCAGGCTTGTTTCTTCTCCCAGTGTGATTTACACAACACAGCATACATAAATTAGGA
    CCTGCTGGCTAGGCAAACCAATAATTACAAGCAACTTCAGGGAATCTCTAATGCAGGAAACATCTACAGA
    ATTTCAGATGAAGTATCTATAATTTATGCAATGACAGTGCCCTCTGAACTATTTCATTTAGAAAGCAAAA
    TGCAAGTCACTACAAATCATAGAGCAGCTATGGTTTCTGGTGATCATTTTCACATAATCTACATGAACAT
    CTTGGCACTGTCTCATCAGTTGACATATCTTGACCTATGAATTTAATTTGGCCGTTGGCACTGGATATTT
    TATACACATTCAGTGTAAGGTCAGAACCATGGGTAAAAAGACAGCTACAAGCATTCTACCTGTTGGGTCT
    GTGATCATGCCACATGCTGTATTCTTCTTTGCTTCACTCTCCAGCACAGCATCACATTGGTCTTCTGTTC
    CGACAGAGCACCTTGGGAAAGCAAAAAAATGAAGTGTTTATATACATCTCTTATTACCAGATCTTTTGTG
    GAATTCTGACGCACAATAACCCCACATAACAGTGCATTTTGTTACAGTCAGAAAAATTTATCTAGCTTTT
    TTGAAAGGATGATGTGAGCCCATTTCTGAATGAATGCCCTTTTCAATGCCTTTTGGGCCAATCAAAAGAC
    TGTTCTTGCTGAAAAAAAAAAAAGAGCTATGGGATCCTGTAAGTTAATGATTTCCAGTCAGGGATCTGGA
    CAGCAATCACTCCTGACTCCCATAAACAGTTCTTGCTTTGGAAAACAAGGCTTAAAGGATGCAACCCCTC
    TTCTCATTCCCTCTTTAATAAGAAGAGGGAGCTCCGTACTTCTAGTGGGTAATTGGAAGATGCAGAGTAG
    TACCTGGATGACAAAGCCAGTGAATGGATAGATATTTGATTTCATAGAGCTTGATTAGCAAAAGTGATTA
    GATTCCTGTACATACATGGTGTTATTCTCAGGTACAAGCCAGCTTTGTCCAAGTTCATTGGAATTACTTG
    CAGTGTCACCGTTGGGCTTTATGTTGTCATCATTTGGATCACCATTATAATTACCTGTAAGGGTAAAAAA
    AAAAAAAAAAAGGATTTATAGGATACATGAAGTTCACACCTGTAGAACAAACATAGATTTCAGGCTATAA
    AGGAAAATGCAATGAACTAGTGAGAGTTTATATCCAATGCCAATGTGCACCGGGCAAAATAAGAAGAGTA
    CTTGTATTTGGATCTCATCTGTAGTGTTGGACTAAAAATCAAATATTATTGTCATTTGCATCATTACATC
    CTTGGGTAGGTTTCCTGAATTGAAAGTGAGAAACAACCACACAGAGGTAATTTAACTAGAATATTTCAGA
    TGATCAAGATCAAAGAATAATCAGAAAATCAGAAACTCATAATACAAGCTACTCTTACAATTTCTACAAT
    CCCATTTCCATCCTGAAGTATGTTTCATGTAATCAACTGGATTACAGAATGTTGTTCCAACTAAACCTGC
    TCAGATTCAGGCATTGCCAACGTGACTGATGAAAAATGACTGATTGCTACTAGCACTTCACTCTCATTAG
    CTACAAAAACCATCTGTGACTCTCAGTTACTTTGTGTTCTTACTGTAAAAGAACTTCTTACTGTAAAAGA
    ATGTGGTAGAATGTGCCCTTTGTGTCTGACCTGTTCTTTCTCAAAGGACTCTCAAAGGGTTCTATCTTTA
    GTGTTGTATGTTAATGTTTTGTGGCATTTGAATTTTTGAAACTACATTTTTTTTTAATCTTATTCATCAA
    GTCTATCACCTGAGGATCATCCATACTTGATTCCTTCATTGCTTTGTTTTACTCTAGACATTACCTATCT
    AAGGTAGAATAGAATCATTACTCACTCTAATTTACACTGATAAGTGAGTTTTGTGCTTTTTTTTTTTTTT
    AATCCAGCTAAAAATATTATTTGTAGCTATACTTCAAAGCATTCCAGACAGATAACATGTCACTTGATGC
    TTTGTAACAGTGTGCAGAGACTGAAAAAAATCAGTATTTTTTCTTTGCTGCTGTAAATTCTAGTTCCTTT
    GGTAAATGGTGGTTTTATGTAGATTAAGTATGAAACTAAGAAAAAGGTTTAAGTAACCACTAGTCTATAC
    AACTCACTAAAAAGTGATTAGTTCTGTGACTTACCACACAGGCCACAGAGCAGGCCACTGTAATCAGATG
    GCACAGAGACTTCTGCATAGTGATTTCCATCATATCTCACCCACAGTCCAAAGTCAGTTTCCAAGAGAAC
    ATAACTACCACTGTTTTGAATACTGATTTTCTTTTCAATAAAGATGGGTAGGTTCATTCTGCTGCCATTC
    ACCTACAAACCAAGTTGTAAAAGTTAGTGTATAGCTGCTGAAACTAATCCAACTACAAGCAACTGGAATA
    GTTTAGTGAAAGATGTCATTTTGTGCACAAAGAGAATGGGACACAATTAGATAATATTTCACCTTAGCAA
    ATTTTTTTGAAATCTCATTAAAAGAGGTACATGTATCCAATGACAAGAGAAAAATTACTATAAATTCTAC
    AACAAACAAACAAACAACAAAAAAAATTAATTATCTGCATGTTAGTTTAAAGATGTATTTCAAACAAGTA
    TTTTCTTACATTCACTTTCTTGCTTTTCAGCATAGAAATCTGGTAGCCATAGACTTCCACTTGCACTGAC
    TTCACATAAGAGACTTTGGTGTTGGATCCTCTGTGCTCATTTGTTGTGGACACATCAAAGTATGGAATAT
    TCTGAGAGATGTTACACACTTTGGAGAGGGTGTAAGAACAGTTTCCCATGAAATTATGAACTCTGTGATC
    AAAAGTGTTGTAATGTGGATCCCCAGAGGCACTGCAGGAAGCATGGCCTGGGAAGAAAGACAGAATAGTT
    TCCTCTGAATCTCTGCTGTAAATGCTGCAGTCACTTTGTTTCACTTCCTAATTGCTGTTCAGTTGTGATA
    GTGATGGCTTCAGTCAAGAACAGAGTGCAAAGGAGGTATGCAAACTTTTCACAGGAGAGAACACCCTTCT
    ACCATAACTGGAAGTCACCCACATTCTGTGACAATTCATGAAGAGCAAGCCATCTGATCCAGAGACTCAT
    TTTCTGTATAGTAGAGACACACCATATGAAACATGATACCTAGAAGTTGATCTGAATCATGAACATCCAA
    ATAAATAGGATTTTCATGCCAATAACTACCTTGAGATTGAAAACAAGACATTCCAAAACCTGGGCTAAAT
    TCCAGTCTTTGTACCCCAAAAGAGTATGTACTCTGGTGTACCAGAAATCTCTGCTAGAGGCCAATGAAGA
    AATATGAGAAAAAGGATATTAAGACATCAGTATATAAACTGGAACAGTGAATTTGCAGTGGCCAATTCTG
    AAAGGCTATCTCTGAATATATAGAAATAATCTACGCAAAAACATTACAAGACTGGCTCCTCTGTTTTGGC
    AGCTGGAATGAACTGCTGCAATACCAGCTGTCTAGAGTTCTGTAGTTATATCAATTATAGAAATTAAGGA
    AAACCTCTGACACTCCAGCATAACTCTGTTAATTCGGCTGTTAGACCTGAAGTTAGAAAACTGAACAAGT
    AAAATAGGCAAAAAAGAAAAAGGAAATAGGAAACAAAAACACATGAGCTTAGCTGGGGTGTGGATGGCAT
    TTGGGGAAGCTTAGAAGAGCTGTCAGGATATGGGCTTGTAGGCTTTGGGGTTGATGGAACTGCAATAAAT
    CAGAGTTAAAGGAGGTCTGATTCATCATGAGACATTTGCTCCAAGTGCCTGACACAAATTGCATCAAAAT
    TCTCTATAATGGCAAAAATTACCACAGATAAATAGAGATAGTGAAGTGAAAAACTCACTGGAACTTGGTG
    TAGGTGATGGAGTTGGTAAGTGTGAAGGTGGTGTTGAGATGTCTGGTAAGGGTGTCCCAGGTTTTGGAGT
    TGATGGACCTATTGTAGGAGAGGTATAGCAGAAATTAGAATCAATATGCAGTTTTACCCCCTGAAGATGG
    TACACCCTTTCCAAACACCTGAGTGCCATGACAGTATTAACCCAGGACAAAATATACACAGTAACTATTC
    TAAAAAAACTGTGCCAATCAAGTACTCCCCGAGCAGTATGGCTGGGGGAAAAGCAAAGTATAGACCCTTA
    AAATGGCAATTCCAAACCCTAGCCAGGGACCTTGAAGGAATCGAACAAAAGAGATGCCAGAAAATGTACA
    AAATGGGCTCAGGTCATGACAATACCTGAGCTTGGTGTGGTTGTTATTGGGTGTGGGGGCGGCGTTGAGA
    TGTCTGGCAAGGGTGTCCCAGGTTTTGGTGTTGATGGTCCTATTGCAAGAGAGAGGTAGTGGAGCTTAGC
    ATCAACATACTGTTTCATTCCCAGTTGCCAGTATGCCCTTCCCAAACACCCCAGTGCCATGACAGCAGAG
    GTACTGAACAGGGCATACACAGGGAGTCTTCAAAGAAAACTCTGCTAATCAAGTACAATCCCAGCAGCGA
    GGCTAGGGGAAAAGGAAACCACAACCCTGTTTCTACAGCCATGCCAACCCCTAGCCATGGACCCTGATGG
    AAATCAACAAAAGAGTTGTCAGAAGCACTGCAAAACCGGTTCGCGTCATGACAATACCTGAGCTCGGTGT
    GGTTGTTATTGGGTGTGGAGGCGGCGTTGAGATGTCTGGCAAAGGTGTCCCAGGCTTTGGTGTTGACGGT
    CCTATCGTAAGCCAGAGTGAGAAGAGATAATATTCAATATGCTGTTTAATTCCAAGCAGCTGCAAATGAC
    TTCAAAAATACCACAGTGCCATGACTGCATAGGCAAAGAACAGGGCATACACAGGGACTCTTGTAAGAAA
    AACCTGTGCCACTCAAGTACACTCCCAGCAGAGAGGCTGGGGGAAAAGGAAACCACAACCCTGTTTCTAC
    AGCCATGCCAACCCCTAGCCATGGATCCTGATGGAAACCAACCAAAGAGTTGTCAGAAGCACTGCAAAAC
    AGGCTCGCGTCATGTCAGTACCTGAGCTCGGTGTGGTTGTTAGTGGGTGTGGAGGTGGTGTTGAGATGTC
    TGGCAAGGGTGTCCCAGGCTTTGGTGTTGACGGTCCTATTGCAAGCCAGAGTGAGAAGAGATAATATTCA
    ATATGCTGTTTAATTCCAAGCAGCTGCTAATGACTTCAAAAATACCACAGTGCCATGATCGCAGAGGTAC
    TGAACAGGGCATACACAGGGAGTCTTCAAAGAAAACTCTGCTAATCAAGCACAATCCCAGCAGAGTGGCT
    GGAGAAAAAGGAAACCACAACCCTGTTTTTACAGCCATGCCAACCCCTAGCCATGGATCATGATGGAAAT
    CAACCAAAGAGTTGTCAGAAGCACTGCAAAACAGGCTCGTGTCTTGACAATACCTGAGCTCGGTGTGGTT
    GTTGTTGGGTGTGGAGGTGGCGTTGAGATGTCTGGCAAGGGTGTCCCAGGCTTTGGTGTTGATGGTCCTA
    TTGCAAGAGAGACGTAGTGGAGCTTAGAATCAACATACTGTTTCATTCCCAGCTGCCAGTACGCCCTTCC
    CAAACATCCCAGTGCCATGACTGCATAGGCAAAGAACAGGGCATACACAGGGACTCTTGTAAGAAAAACC
    TGTGCCACTCAAGTACACTCCCAGCAGCGAGGCTGGGGAAAAAGGAAACCACAACCCTGTTTCTACAGCC
    ATACCAACCCCTAGCCATGGACCCTGATGGAAACCAACCAAAGAGTTGTCAGAAGCAGTGCAAAACAGGC
    TCGCGTCATGACAATACCTGAGCTCGGTGTGGTTGTTAGTGGGTGTGGAGGTGGTGTCGAGACGTCTGGC
    AAGGGTGTCCCAGGTTTTGGTGTTGATGGTCCTATTGCAAGAGAGAGGTAGTGGAGCTTAGCATCAACAT
    ACTGTTTCATTCCCAGCTGCCAGTACACCCTTCCCAAATACCCCAGTGCCATGACTGCAGAGGTACTGAA
    CAGGGCATAATCAGGGAGTCTTCAAAGAAAATTCTACTAATCTAGTACAATCGAAGCAGAGAGCCTGGGG
    AAAAAGGAAACCACAACGCTGTTTCTACAGCCATGCCAACCCCTAGCCATGGACCCTGATGGAAATCAAC
    AAAAGAGTTGTCAGAAGCACTGCAAAACAGGCTCGCGTCATGACAATACCTGAGCTCGGTGTGGTTGTTA
    GTGGGTGTGGAGGCGGCGTTGAGATGTCTGGCAAGGGTGTCCCAGGCTTTGGTGTTGACGGTCCTATTGC
    AAGCCAGAGTGAGAAGAGATAATATTCAATATGCTGTTTAATTCCAAGCAGCTGCAAATGACTTCAAAAA
    TACCACAGTGCCATGACTGCAGAGGTACTGAACAGGGCATACACAGGGAGTCTTCAAAGAAAACTCTGCT
    AATCAAGTACAATCCCAGCAGCGATGCTAGGGGAAAAGGAAACCACAACCCTGTTTCTACAGCAATGCCA
    ACCCCTAGCCATGGACCCTGATGGAAACCAACCAAAGAGTTGTCAGAAGCACTGCAAAACAGGCTCGTGT
    CTTGACAATACCTGAGCTCGGTGTGGTTGTTATTGGGTGTGGAGGTGGCGTTGAGATGTCTGGCAAGGGT
    GTCCCAGGTTTTGGTGTTGATGGTCCTATTGCAAGAGAGAGGTAGTGGAGCTTAGCATCAACATACTGTT
    TCATTCCCAGCTGCCAGTACACCCTTCCCAAATACCCCAGTGCCATGACTGCAGAGGTACTGAACAGGGC
    ATACACAGGGAGTCTTCAAAGAAAATTCTACTAATCTAGTACAATCGAAGCAGAGAGCCTGGGGAAAAAG
    GAAACCACAACGCTGTTTCTACAGCCATGCCAACCCCTAGCCATGGACCCTGATGGAAATCAACAAAAGA
    GTTGTCAGAAGCACTGCAAAACAGGCTCGCGTCATGACAATACCTGAGCTCGGTGTGGTTGTTAGTGGGT
    GTGGAGGCGGCGTTGAGATGTCTGGCAAGGGTGTCCCAGGCTTTGGTGTTGACGGTCCTATTGCAAGCCA
    GAGTGAGAAGAGATAATATTCAATATGCTGTTTAATTCCAAGCAGCTGCTAATGACTTCTAATATACCAC
    AGTGCCATGACTGCAGAGGTACTGAACAGGGCATACACAGGGAGTCTTCAAAGAAAACTCTGCTAATCAA
    GTACAATCCCAGCAGCGATGCTAGGGGAAAAGGAAACCACAACCCTGTTTCTACAGCCATGCCAACCCCT
    AGCCATGGACCCTGATGGAAACCAACCAAAGAGTTGTCAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
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    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
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    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
    NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGTCCGGT
    ACTCACCGTTCACCTGGGGCAGACACAACGCAATGCACCGTGGACTCCCTGGGGCCGTCAGCCCCATGGG
    GACTGGCTCCCTTCTCACACAGAAACTCCTGCTGCTTGCAGGTAAGACCCTGCCCAAAAAGACCAGCCTT
    GCCTTGCAGACCCACCGCGCACACGCCAGACCCACTCCCTTACCAGCACTCGGCTCCTCTCCTGCTTGAG
    TATGGTAATGCGCTCTCCATACACATCGACCACGACTTCACGCACGTAGGACACTCGGGGGTTGCCACGA
    TGCTCGTTCTTTGCCTCAACGTTGAAGTACGGCACAGAGGTGTTGGAGCACACTTTGGCCAGGGTGTACG
    TGCAGTTCCCCATGAAGTTGTGCGTCACCTTGTCAAAGGTGTTGTAGTGAGGGTCCCCGTGGACGTGACA
    GATGCCGTAGGAGTGTTCCAGGCACTCGGGTTTCCCATTCTGCACACCACAGAACTGGCCAGCAGGGCAC
    GATGCACTGGAGCACTGGACTTTGCCTCCTCGCGTGGGACATGTGCACTTGGACGAGCAGGTGTTGTCCG
    TCCAGAACTCTGAGCCCACCGGGTAGTGCTGCCCGTTGTGCCAGCACCCACACTGCTGGATGGGCACACA
    CTGACTGTTGTAGAGCAGGTACCCGCTGTCGCATACACATCCCTCCACGCACGGCAAGCTGCAGTTATAT
    GGTGCCGTTGGGCTAACACACGTAGCAGGGCAAGCTGCAGCACAGGGCTCGTAGTGGCTGTTGGCTGGGC
    ACGTGATGGCTGCCAAGACAAAAGGAAAAGCAGTGAAATCATCAGTCATCCTACACCCGCAGTGCTCCCA
    AGGTAGGCCAGATTCACTGGGGGCTTTGGAAGGATGCAATCGCCCCACAGCTCCTCCCAGCTGCCCCAGT
    GGGATGCCTTCTACATAGAAAGAACCACACCGGCAATGTGACCAACAAAGGGTGCTCCCACACTCACAGC
    AGCTAACCAGCTCAGAAAATGGGGTGCTTTTCTGTTTCTGACTTACGGCAGAATGTTGCGTTCCTCCACA
    CAGGGATCTGGACACCAAGCGCCTGGCAGGCATCTGCATAGGACTGCAAGCTGTTGCACAGCGCCTCGCG
    ATCAAGCCCCAGCTCACACAGGTCATACAGGCACGTGTCGAAGTAACCGCTGGCACTCAGCACGGCATGG
    CACACCTCAAACGGGCCGCTGGTGTCCGTGAGGAGCCCACAGAAGTTGCTGCTGGCAATGACTTGCTTGT
    CCGTTTCAGTGCAGGCTGGCGGGGACTGCGTCCCAGCTGAGCAGCTATAGTGAGGCAGAGAGAACAGCCC
    CCGGGTTATCTGTGGGCTTCTCCTGGCAGCTCCACTCCACCCCTCCCCAGACCTGCTGGGGCACTACACT
    CACCTGCTGTCATTAGACACCTGCCAGCTGTTACCCAGGCTGGTGGAGTCTGGCTCCAGCACCCCGTCAG
    GGTTCAGGAAGTCGTCTGCTGCCATCCCGTTGTAGTTGCCACACATGCCACAAACCTTCTGCCCAAAGGT
    GCTTGGAAGCGTCACCTCCACCCGATGGTTCCCATCAAACTTCACTCTCAAGCCAAAGTCTGTTGTCACC
    ATGGTGTAGAACCCGCTGGACGAGACTTGCACGCCCACAGATGGGGTGCAGGGCAGCACCTCTGGCTCGC
    CGTTGACCTGGAAGGACAGAAACCCAGACAGGGTTGGAGCAATCCCTTTGGCACGGCTCTCCCCCGGCAC
    AGCCCCGCCACTGCTCCCCTTTGTGCTTACCGTCACCACGCCACCCTTCCCAAGCCTTATGCGCTGGCCG
    TACACATCAACGTCCACGTACTGGACGTAGGACACGCGAGTGTTCCCTCCTCGGTGTTCATTGGCAGCTT
    CCACGTTGAAGAAGGGCAGTGAGCTGTTGCTCTCGCACAGCTTGGAGAGGGTGTAGGTGCAGTTGCCCAT
    AAAGTTGTGGATTTGCTTGTCAAATGTGTGGTAGTGAGGGTCTCCCTCCACCACACACGTTCCTGACAAA
    ACAAGCACCAGAGAAACCCTTCAGTTAGGCACCTCCTTCCAGCGCTTCTCCCCTTTCTACAGTGCTAAAA
    GCACTGCGTCCCAACGACCGCTCTCCTCCATTCACCTGAGCCTGGCTGTGGTGTTGGTGTGGTTATTTCT
    GCCACAGGTGTGGTGGTGCCGGCGCCTGGTGTTGGGGTCACATCGCCTACAAAAGACACAGTGGCACATG
    CCAATCAACCCTCCTTGCCCACGCCCTGTTCAAGGCAGAAAATGCACTGACTGCCACTACAAGCAGGCTG
    CCTTTCCCCAGCCAGTGTCACGTACCTGGACAGTATCCCTGTTCAATGGACAGATCATCCAGTGCTACGT
    CACTGCGGAAATCTTCACCTATCATGCCCTCAAGGAGAATCTAAGAGAAGGGTTTTTTTCCAGCATTATT
    TCCAGCCATATACACTGAGTGCTTTTTGAAAGCGCATGGCAGTTAGAGACCCTGTTCCTACCTGCAGGTT
    TCCTGCACTGTGCACTGTGACCTCTCCCAAGATCCATCTGTCTCCTTTGCTTCCTGCTTCTTTCCATACC
    AGTGTCGATATCTCGTTGTGTAGAACGTACACCCGCAGGGCCATGGTTTCAGCCACTCCGTACATGTGAT
    ACCAGAAGCGGAAGCAGTGTGGTCCCGTTGAATTGCAAACTGTGCTGACCAAGATGGCTGTCTCACCTGG
    CTTGTGCTCGTTTCCTTGCAAGTAGATATAATACCCATCTGGAAATAACACAGTGACAGTTTCTCATATT
    GTTGAACCAGGAGAAGGCTAATTTACTTCATTTACATTTTTTGTGCTAGTACAGTTTTCTGGGAGATATA
    ATGACAGTAGTCAGCAAAAAGTATTCATGCTACTGAGACTAGATCACTTATCAACAGAACAGGTCGTGTC
    TAGACAGCTCTGGCCAGTACGAGTGGGGTGTGGGAGTATGAACAATTAGCCAGTCTGTAATACTTCAGTA
    TACCTCCACCCAGCACTGCCACTAAATTTCTGCTTCCCAAATAGATTTCCAGCAGATTAAATTGTAGTTC
    TGCTTTGGGAAAAACTAGCATCTTGAAGTACAGGCAGGCTCTGAAGTGATCCAGTGTCAGCCTGCATATG
    GTTTACTGAAGCCTTTCTGCAGCTGAGTCTACAAACATATGTTACAAACATTACATGCCCATAAATGTGC
    TGCACTGTTCATCCACCCCAAGACAAATTCCTGGCTGCTGCAGAAAGCTCTGGACAATGCTCAAGAAATA
    AGTTGCCTGGATTTCCTGTTAGAAGAGCCTCTTTGTGCTCTTCCCAAAGATTGCTGTATCTGAAATAGAA
    CCAGATAGCATTTTCTGTTCCTACCTCCTGTTGTGTGGTCATGGGGAGGGCCGGTATTTGGTGAGAGTGT
    TGGTCCCTTGTTCCTTATCCAGTCAAAGTTGTCATTGATCGCCTGTTCCCACTCACAGAAGTCAACATCA
    AATGTGCAGGACATATTGCAGGCTGTTGGTGCAGCTATATATGTTGCAGAAAGAAAAGCAGTTTCCACAA
    TGAGGTCTGGAATCTTCTCTCTGTTCTGATAATATCCTTTCAGATCTAATGCATTCATGTCCATTAAACA
    TTGGAAGATGTTATGGAATGAGTAGAATTAATCCATACAGAAGGATGGATAATAAAATATGAAATTCTAA
    GTGTCTGAAAGTGTCTGTTATTTGGCAAGTCCGTTATTCAGTTGTTACCATAATGCTCTAATATCCTCAG
    TTTTTACACTGTGCCACAGTCTTAATCTTTGGTCACTCAAAGCTCTCCTACTTGGAACAGTGGTATTCAG
    CTGTTCAAAGTGTAGCTTGGGTAGAAATCTAAACACTGTAAATGGGAATGGTCTGACAGTTTTTTTATGG
    TTTCTAACCTGCGATGAGAAATGCTGTCTGAGCTAGAAAGAGGTTATGGGATGCCTTTTGATATCATAGA
    CTCATCCTGTTCCTTCTGCTGGTTTTGGCAGTCACATCTTGTAGTACAGAAGAAGCAGTTTCATGTCTCC
    AACTTTACTACTGTGTTGACTTCTATGTCCACGCAGATAATAAGGGTCAAACAGTGTTATGTCTGGGAGG
    CTAAATGGCCTTAACTTGCAGGATACCTTATTAATTTTAATACTCTATACAATTCTTAAGTTATTTCACA
    TTTGGTGCAAATCTGGAGCTGTAGACCAGTTCTGTAAATGTTGTATTAACCCTTCTCAAAAATGTGAAAT
    ATTTTAATGGAGCCAAAGTGGTGATTTGCTTGGAAATAACACAATAACTTGGTTTGGAAGAAACCAATAA
    GTGCTTAGTTTTCCTTGGGTGATCATTTAACTGGCAGCATATGCCAGCTTTCATGATCCAATAATGAACA
    TGTTAAAAAAGTTTAAACATTATGGCTTAAAGTGAACACTGAATGACCATTACAGGTGTAGATTAGAAGA
    CTGATTTCCTCAGATGCTAAAAAACCAAAATATTTCTGTCTTCCTGAATTGCCCTTTCAGAATTTCTGTT
    CAGCTCTTTAGTCATTTGAGTTTACATTTGTAACATGAGGATATAATTGGATGTCTATTTGCTGAATGAC
    CTATAACTTACCGCAGATGGTATCTTTTCTCCATTCTCCCAGATTTACACCTTCAAGCTCACAGGCAGCA
    GCATATGCCTCTAAAATTTTGCAGAATTGTTCAGAATTCCCCTCTGTGGCACACAGGTCATAGACACAGC
    TCTCAAAGTACAGCTGTGGTGGAATGTACCAATGGCAAGCCTCAAAAGGTCCACTATTTTTCAAAATAAT
    TTTACAGATTTCTTCGTATTCTTTCTCTTTTCCTGTGTCACAGGTGGGTGGTACAACTGCAACTGGATTA
    CATCTGTATTAAAAATAAACAGCAGTATACTTATATTTTGCTCAACCCTGCAAGGTAAACCTCAAGAGTA
    ATAGAACTCTATGCCAAATTCTGTGGACTTATTGTGTATTCTTGCCTTTAGTTTGTCAAACCCCAAAGTG
    AGAAATATTGTAATTCGACCCACAGTTCCCCATTAAGAAAACAATAGCGAACTGTTGAGATGTAACCACC
    TGTCCATAGACAGAAGTCCAAATGGAATCAACCATCCAGATATTTAGCTCAGATAATTTCAGTTCACTAT
    TGCCCATAGTAAGATCACTACTTTGCATATGAAACCATTAATAGGCAAAGAAATGCTCTGTGTCCCACTT
    AGTCAATGTAGAATTACATTAGAAATAGAAGCTGAAGAAAATATTTAGCTTTCTTACAGTTAGATGGTAT
    AAACAAGGTATTTTAAAATAGAAAGATAAGAAGAAATGAAGTTAACATCCAAGAAAAGAGCTTGATTTCA
    AATAAACTTTATGTAGTTCATAACAAAAATCTTTGAAGGGTAATTTTAAAATTTCAGTGCTTTCTACTGA
    AATGTAGAATTAGTAAAAAGGTTAAGATTCTAGCCAACATAATATCAAAAAAGGGATTCTATGGAATGCA
    TATCATGTATTTTAGTTAATCTCTTAAGCAGATCTATGACATATTTTGTTGTTTCCTATAATATTATCAT
    ATACTTACACCCAGTTGTCATCCTTCAGTAGCCAGCTGTCACCAAATTTATTTGGATCCTGTTCTAGGAC
    TTTATCCGGTTTTAGAAAATCATCCAGCTGGTTGTCATTGAATGTCCCACACAAACCACATAACTGTCCC
    CTGAGACTTTCATCAACCTGAATAAAAAGGTCCTGGTCGCCATCGAACTTCACCTGTATCCCTACAGAGG
    TATCAATGACTACATAACGTCCTTTTACAGCTACTCTGGCTCCATTCTTCAAATCCACAGGAGGGAAAAC
    CTGAGCTCCATCCACCTAAAGAAAAGAAAACCAACACAAATGGGTAAGTCAACAACGTTCCTAGGAAAAA
    AAGGAGAGATCTACACTGATAATACTGCAAGTTTTCTTCATTGAGTCAGCCTTATCCTAAGGAGCAATGT
    AGTCACAGAAGTTTGAAGGGACATTCATACTTGTCCCATATCTTGAATTCAGGTTTGTAGAACAACTTGA
    GCTCAGCACTGTTGCAACTACACGTATAGCAATACTTGGAATAGCTAGTTTAAAGCCAGCTCTGGTATGT
    AAGAGAGCATATAGCAAAAATGTTATTTCTGTTCTAAAGACCCAAACTGTAAAATGCAGATGGGCATTTA
    GTTCAGCAAAAGCTTTCTATTAAGGATAAAGCTCCAGATGGAGCTGCTTTTTCTAAAATGTTGTGCATAA
    AATATTTGAAAGGATATAATGAAGCTATGCTGATATCTACATTTCTGAAAAAATATTTCAGTGACTTCTT
    TCATATATATTCTTGGAGGTGAATAAAGCAGAGAAACCTGATTCTGTTATTTTGGATGGAAAAATGCAGT
    GCACTGATATGACTGATTATTTGTGCTTAAAGTGGAACACTTACAAAGGTCTCTTTGTCTCTCCTCACAA
    CAATATGAAGATTTTTCAGTGTGACAGCAACTGAATCCAGGGCTGTCCAGTTTGGGTTTCCTCTATGCCT
    ATTTCTGGTTGTCACTGAAAACCATTCAGAAGAGTTTGTGCAAGTCTCGACAACTGTATAATTGCAGTCT
    CCTTGGAAATGATACAGTCTCCCATCAAAGGTTACGTAATGTGGATCCCCGAAAATGTGGCAGGTGCTGG
    GATTTAATGGATAACACCCCAGGAGTCCATTCTGCACCTTGCAAACTTCATTTGCTTCGCATGTGTCTGA
    ATTGCAAAGCACAGTCCCATTTCCAACACATATACATTGGCCTGCACAGTTTGTTTGCCAAAAAGTTTCT
    CCTTTCTGTAAGAGAAGACAAAATAAAAAGAAGTTAAAACAGTTCATGTTAATGATCAAATGATCAAATG
    TAAAATAAAACCTCTGAAATATGTGATAGAACAAACCTCATAATATTTGTCATTCTGTAAGCAGCCACAG
    TTGCTCATAGATACACATTGTGCCCCACTGATGACAAAACCATCGTTGCATTCACATCCCTCTACACAAG
    GTTTGCTGCAGTTGTTTGATGCAAGAGGGTCAGTGCAGGTTGCAGGACATGCACTGGTGCATGGATTGTA
    ATGGCTGTTAGCAGGACATGCAATAGCTGAAAAAGAATAAAAGGCTCTCAAATGATAATTACAACTCACA
    TCAACCCAAAATATGAAACTGAGATTATTTTAAAATACTGGCTATATTTTATCAGAAATATTATCAGAAT
    ATAAAATTTATTTCCTGGTTTGAAATCAGAAAGAAAAATAATATTCTACAAGAGAAGGGCATGAATATTA
    ACATATATTGTTGAAATTTAGCAAATTCAAATCAAAGAGAAAAATAAGTCACTCTATAACAGGTCCAGTA
    CTTAGTTGGTACATGAATTATTGAAGCTATTAAATGTAGACAGGATACTAACAAACATATTAAAAGCAAA
    ATATGTGATAAAACACAGAGGTGATTACATACTATCATGGGCTTACTGCTAACTCTATGACATAGATTCT
    TTCAATAATGAGCACAGTTCTCGAAAGAGATCTGGCAGTAAAAAGAGTGGAGTGACCGTTGTAGAAGATT
    ATTTCTCAGCATTTCATGGGGGAATGTAAGCAGAATTTAAGATTTCTAAGCTATGTCTGTTAACGCCTGA
    AAGCAGACTTTTGAAAATGGAAGGAACTATTCTAAGATTCAGTTCTACATAGGTACTCACGGCAGAAGGT
    GGCATTCCTCCAGGGGAGCAGAGTCACACCAGCTGCCTGGCATGCGTCAGCATAGGCCTCCAGCGCACCA
    CACAGCACCTCCTGCGCACCACTCATGGCACAAAGGTCGTAGGAGCAGGTCTCAAAGTAGCTCTGCGGGT
    TGATCACGGCGTGGCAGCTCTCAAAAACGCCTGGTCTGGCTGTCAGCATGCCACAGAACTGCTCGGACTC
    GTAGAGCTTCTCCTTGTCTGCAGGGCAGGGCTCTGTTGGTGGGGGCACGCCGCAGCTGGGGTCACTGTCA
    CTGCTTGGGACCTGCCAGCTCTCCCCCAGCGCATTGGAGTCTGCGGCTTGCTGCCCGTTGGGCATCATGT
    AATCGTCCTCTCTCCGGTTGTTGAAGTTCCCGCACATGCCACACGTCAGGTTGAAGTAGCTGCTGGGCAC
    CTTCACCTCCACCGAGTGGTCAGCGTCGTAGGACACGTGGAGGCTGAAGTCCGTCTCCAGGACGACGTAG
    CGGCCGCTACTGCTCACGGTGATGGCTCCTCCTGCTGCGCTCACCGGCAGGGTCCGGTACTCACCGTTCA
    CCTAGGGCAGACACAACGCAATGCACCGTGGACTCCCTGGGGCCGTCAGCCCCATGGGGACTGGCTCCCT
    TCTCACACAGAAACTCCTGCTGCTTGCAGGTAAGACCCTGCCCAAAAAGACCAGCCTTGCCTTGCAGACC
    CACCGCGCACACGCCAGACCCACTCCCTTACCAGCACTCGGCTCCTCTCCTGCTTGAGTATGGTAATGCG
    CTCTCCGTACACATCGACCACGACTTCACGCACGTAGGACACTCGGGGGTTGCCACGATGCTCGTTCTTT
    GCCTCAACGTTGAAGTACGGCACGGAGGTGTTGGAGCACACTTTGGCCAGGGTGTACGTGCAGTTCCCCA
    TGAAGTTGTGCGTCACCTTGTCAAAGGTGTTGTAGTGAGGGTCCCCGTGGACGTGACAGATGCCGTAGGA
    GTGTTCCAGGCACTCGGGTTTCCCATTCTGCACACCACAGAACTGGCCAGCAGGGCACGATGCACTGGAG
    CACTGGACTTTGCCTCCTCGCGTGGGACATGTGCACTTGGACGAGCAGGTGTTGTCCGTCCAGAACTCTG
    AGCCCACCGGGTAGTGCTGCCCGTTGTGCCAGCACCCACACTGCTGGCTGGGCACACACTGACTGTTGTA
    GAGCAGGTACCCGCTGTCGCATACACATCCCTCCACGCACGGCAAGCTGCAGTTGTATGATGCGGTTGGG
    TTAACGCATGTAGCAGGGCAAGCTGCAGCACAGGGCTCATAGTGGCTGTTGGCTGGGCAGGCTAGTGCTG
    TGTAATGTAAAAGATTAATACAGAAGTTTATATTTTAAATATTGAAGTATTTGGGTTTATTTAATGGCAT
    ATTCTGTTTTGTTCTGCAGTCTTGAGGACACCCTGGAATCCTGTGGCAATGCAATCCTTGCCTTTTCTGC
    GTAAGTGCCCTTTAACATAGTTCATGGTTGTGGCTCCTAACAATTTCTCTGTCTGAGAATTTCTTATGAG
    ATCTTCTATTCTGGTCATTGTTTGCTACTTTTTCCAGCTGAGGATATCTGTGTCATGTTATTTGTCCTTG
    TCATGTGCCCCATTCAGGCTACAGGTCTGATAATTCTAATCATATCATAGCTTCTGAATTCATACTTTTT
    CAATCCCTGAAGTTCTTTAATGTTTGTTAAATAATCGTGATGTACATGCACCCTATTCTGTTTTTATACC
    TTGCTACACATGTCACTAGTTAAAAAGAAAAACTAAGCTAATTCGAAGTTCTTCTAACCTCAATCTTCAG
    TCTCTTTTCAGATTCTGTACACTTATGTGATGTAGTTCAGTTTTTAATGACATGCCCCACTCTATTTTAG
    TCTGCTTCCTTAAGCAATAGAGATCATATAATTACATTGTATATTTCCCTGTCCATCCTCTCATTAATTA
    GAACTGACCCAGAAATAAGTGAGGTTGGCAGAGGACAAAGGCATAGTTTGAAACAGGAGTAAGGCAAGCA
    GGAGAGACCCGCAAGCTCCTGCAGCAGAAAGAACAGCTACAGAGAGGGTTTATTCCTGCTTAGAAATCAC
    GGAATTTATGTGGGAGTAAGCCTTAATAATATCCTTAGCAAATAAAATGCTCCAGCAAGAGATTACAATT
    AATTGTGAAACAAATATCCAGAGTAAATTTGGCTTCAGCCATTTCAGTGTTTGCAGACCAGCATCTTTCT
    TGAGTCTCCTCATCTCCCTCAAGGCAGAGGGCACACAGGCCACAGCAGAATATACTGATTCCCAGATCTT
    CTGCTCAGTTCATCAAGCATTTTTCTGCCTCATCCTGCTCTCTTTACATTGGTATAAATTCTGAATTTCA
    GACATCTTTACTGTTCAGTCTCAGATCTATTCCTCCCACCAGTTTTGATTTTATCATTCCTGCAGGGGCA
    ACAAACTGACCCCCTGACTGGCTACCTAGTCCATGTTCCTTCTACTTAGTTTAGCCAGTCTCAACATGTA
    CTTCCACTTTTCCATAGCCCTGTAAGCATGTTCTCGTTTTCCTTTCTAGAAGCTACTTGCCTTCTCAAAA
    TCCAGGGCCTCCCATTTACCCCATTCAGCATTTCTCTCCAGTTCTTAATCAAAGCTCCTTTCTCATTGCC
    CCAGACCACCTGGATTCCTTATCTACAGCAGCTCTGCCCTTCATCTGCTCTGGCTCATATCCCTTCTGTG
    TTCACATGAGATATCTGTCTTAATGAGCTTCTGCTGGGGTTGCTGCAAGAGAAGGCACAACCTCCTTTCT
    GTTGACTGGGTGCCTGGTCACCCTACAAGGTGAAGAGGCTAAGAGAATAGAGTAAGGTATCTCAGCTTCT
    CTACAGGGGGCGTGGGTGAGGCTCTCACCTCTACTAAGCATGTGCAAAATGAGATTTTTTTTTTCCCCTC
    AAAGACCCTAAAGTTTATGCACAGCTTCCTCAGAAGGTAAAAAACCCTCAAAGTCAAATGTCCCTGATAT
    ACTTAGTACCCCTGAACAAAAATATAGGGTAGGAGAAGAATTCAAAAGAAAAGCTCTCTACTTTCATCAG
    TCTTTCTAAAGGACTCAAGGATTCAAGCTAAGGACTACACATGATTCTGAAATCTTTAGCCTCATAGGTC
    AAGATTTACAGTGTTACAGGAATTTAAAAGAGTTTCTTGTAATGCTCTCTCTTCAGGCAACCCTAATGAT
    ATCCTCCTGACAAATGGTAATTTCCAGTAATCTTTGATACGTACGGCAGAAGGTTGCATTTCTCCATGGT
    GTCAGCTGGACTCCTGCAGCTTGGCACATGTCTGCATAAGCCTGCAGGTTTTGACAGAGGGCTTCGCTGC
    TCAAATGAAGCTCACAGAGATCATACTGGCAGTCTTCAAAATAGCTCTGTGGATCAACTACTGAGTGACA
    ATTCTGGAATGGACCACTGGGATCAGTGATCAGACCACAGTATTTATTGCTTTGGATCATATGTTTTTCA
    TCAGCAGTGCAATTTGGGATATGGCCATCATCTGGCAAGCACCTAGTGTGGAAAAAATAGATGTGGAAAA
    TGTCACTAGTGAACTTTGCATACAATTGCACTCAAGATGCTTTTCAAATCCCAACTAATAAAACTACAGG
    GCTGGCCCAGACTAAGGATGACTTTTAAAATATTTGTATTTCAAATGGGATGGGAATTCAGATTTTAAAA
    TGATCTCAACATTATAACTTTTGTAATGCCTGTAAGAAAGTTAATCATTAATTTTGGTTTTAGGTCTAAA
    AACAAGCTTTGTTCAAGTAAGACAACTAGGAAAGGCAAACAGAAAGTAAAAGCATTCAAAGTTAAACATC
    TATCATCTTGAAATGACACACTGTATAATTATAAAATAAACAGTGAAGTATCTCAGAAGAGTTTTAGCAG
    GATCATTAATATGCTAAAATAGATGGAGTCCTAGTGGGCCATTACAGGTGTATTCAATTTTTCTCTTTTT
    AAGTACACTTGAATTTGCTACAAGTTTTTTTCTTCCCAGATACGTGAGAGAGCTATCCAGGCAAGAATAT
    ATTTTAATCCATATTCCTGACATTTTGGGCCTACATACAATAGCTGCTTAAAATACTGCTCGTTACATAA
    CTCCCAGATAAGGCCAAACATCCCAATTGGAGTCCTACCTTGAGTCATTGTAAACTTGCCAACTGTTTCC
    AAGGCTGGCTGAGTCTGTTTCCATCCCTCCATCTGGGTTAAGAAAGTCATCTTTTTTGTCCCCATTATAA
    TTTCCACATATCCCACAGACTTTTGATATATAGGCACTGGGAAGAGTGATTTCTGCTCTGTGATCTCCAT
    CAAACTTGACCTTCAGCCCAAAGTCAGTAGTAACCACAACATATTGACCACTGAGGGAAATGCTGACACT
    ATTGCCCAAGGTCAGAGGGAGTACCTGGCTGACTCCATCAACCTACAGGAACAGAGAAGGACAATCAGTA
    TAATCACTTTAAAATACGTATTTTTAATAGTGATTTTCTTAGACCATGTTCCTATACAGAGCATAGGGTT
    TGTACAGTGAACCTGTACTAGCATTAAGAACACACAGTGTTGTGTTTCTGGGTCTTTTTGGGCCTTACAT
    AACATGAATGCTATATGTGGACAAATGACCTTGGGCCTCAAGACCGTAGTAATGCATGTACTTATCTTCC
    AGGTCTTAGCTGTGGTCAAAGTAAGCTTGGTGAACTAGACCCAGCTGGAATAAGTGCAAAAATTATCAAG
    GTCTTTCAGCAGTAACTGCTTACAGGCTTTCGGGATCAAACAAGAGCATGGTATAAGATTTAAAAGATGT
    AGCTAGAATGGAGTCTCAGAAAATTTTCAGGGTTGGACATATTTTGAAAACATTTTTTGTTCCTTTTTTT
    TTCTTTTTTTTTTCCCTCTTCTTTTTCTTTCTTGTTAATTTTCACATGTGAAATGCCAACAGTAAGAAGG
    AAGTGTTCAAATAAAATTTGCAATTTATTTTAAATTCAATAAAACTAACATTCTTTGAATATTTGTTCTT
    ATTACATAATTAATATATATACATTTCAACTTAGAAACATATATAATGAAAACAGTAAATACAGTGAAGA
    GATTGAAAAGAGAAACTTTTGGACAAATGCAGATATCAAGTTCACTATGCATCTAAGTTGTGAAGTCTCT
    GTATATTTAAATCCTTATCTCCTGTCCTATTTTCTTTATAAGAAGAGTGATAATGAAGTAAGGTGATCTA
    TTCAATTACTGTGGGTTTTCAATCAAATGTGTATCTGCTACCTTGGCTCTTCATGGAAATAAGTTATAAG
    GAAGCCTTAAAATACAGCAGCTGGTCCAGTGACACAATCAGAGTGATGTGAGTCATCAGGCAGCCTTTCT
    GGGACTCTCATCTGGCTGTGCTTTATGGCAAGTGCCAGGTATTAAGTGAAATTAGCAGTGATGAGGAACA
    ACTAGAAGTCAGAGATGAGCATGCAGCTTTACAGCCTGGGAAATATCTACCAGCACAAGCTCTGTTAATG
    AAAGGCCTTTTTTATTCCCTGCTGCAGACCAAGTTTAGGAATGGAGGTCAATCTCTGAATGGAGGGCTCG
    CTCACCAACTCCACCCAGAGTATCAGCTCTACTCTTAACTGTTAGGAACACAGAAATACAACGTTTTGTA
    TTTTCTTTCTGTTCTGAAATCTCAATGCTTACTTTAAGGAACCCATAGTACTACTGTTCCCAAATATTTG
    ACTGCCTTCTAAATATTGCTTTATGCTTGCCAAGAGATTTGTAGCCATTGTACTACAATTTCTTCAAAGC
    ATGATTTACTGACAGTAGATAGTGAGATCCAGCCTTACCTTAACAACTCTGTTTTTACCCAGATTGATCC
    TGTAGCCATAAACATCAATGTCCACAGATTGGACATAGGACACACGTTTGTTGCCTCTTCTGTGCTCGTT
    AGCAGCTTCCACGTTGAAGTAGGGCAGTGAGCTGTTGTTCTCACACAGCTTGGAGAGCGTGTAGGTGCAA
    GTGCCCATGAAGTCATGACGCTGCTTATCAAAAGTTAAATAATGAGGATCTCCATGTATAGTGCAAGTTG
    CTTCTGGAGAAAGGAAAACAGAGTATGTTTCATTAAGATATTGGTGCATCCAGAAAAGCCAGCTCATACC
    TTCGTTGTGCTGTGGGAAAACATAGTCATCCCCATTTTATAGGTTACACATGAAAGAACTGAAACAGCAA
    AGACAGATTTTTATGGCCCAAGGCAATGCCACAGAAACACACTCAGATAAGTTAACTCAAGTAACACTGG
    TAGAATGCACAGCTGCCCTTCTCTAAAGCACTGACTATCACATGTGATTTGTTAAAATTACCTGTCACAG
    CAGGTGGTATAGGTGCCGTTGGTGTAGGTGTTGGTGGTACAGGGGTGCTGACTGCATCTGTCAGTAAAGA
    CAAGGTAACAATAATTAGCATTATATAACATTACAAACTGAACACTACATACAAACCAACATACGTAACT
    TTCAACTTACTCTTCACCAGTTTAAAGTTAGAATATCTTCACTGAAGAAAATGGAAGTCTACATTTAAAA
    ATGAGGACTTTAGGTTCTTTTGAATTCTTAGGGAAGCTCATCTGACTACTAGCTTGGTGACAGTGACTGA
    GCTTGTCCTTGCATGATTCTCTGAAACTGGAGATGTATTTACAATTGCATCTGTGATTCTCTCTGGTTTG
    TATGTTAAAGCGTGAAGGTCATGTTCTCTGCAGGCCTTGGCAGGTCTGGAAATGAGTAGCCTGCCAGCAG
    ATGCCATGTAACAGAAACAAGTGACTCTCAGGACACTGGTAGTTACTAATCAGTTTAATTTTTTCTACAA
    AATGGCATTTATTTCTTAGGAATGTAATGAATGTAGGAGCTTTCTGCTGACAGATATTATAAACAGTGAT
    GTTGAGTCTTTGCTGATGTCCATTACTGCTTGTTAATACATATTAATACATATTAGCATCTTCTCCACCT
    ATTCACCTATCACAAAGACATATTCTAAAACAAGTGGTCCTTGTGGTGTATTAGTAAGTGTGTGCTAACA
    ACCCCAAGGTGTACTGTAGGCATAGAGCTTAGCAGAAGGAGGAGCACTTCTCTAGTCTCTTGGGATTACA
    TTAGTGCACCATCCGGTGGCTGAATTGTGTGGTAATAAGCCTGGAAAAATGAACAACTGGCTCTCCAAAT
    GGGCCCCAAGTATTTTTCACAATTTTAAAGAAAATTACATCTTCTGAGGTTATAAACATGAATGGAACTC
    TTCATTATCCAAAAATCCTAGCTAGAATAAATTTAAAACATTTCATTGACATTATATGAGTAGTGTTTTG
    CAGAGATGTCTATTAAGACTTGATGATGTTCACAGCATGTTTCTTGTAGAATTCACATCCTGTGAGTTTT
    GCATTCATAACAGTAAAGATGGGATTAAACTGTAATTCATTTCTGGCTTCAAGTCTTCCACAGACTGTGC
    AACTCTAGACTGATTTATTAGAGGGTCAGAGATCAAACTCGAATTAGTGACGTATAATTAGGTATCACAG
    TGCAAGTCAAAATTATTGATTCAACAATATACTTCTCTTTAATTACGCATGACAGACCTGTGTATTTTCT
    GGCTCATACTGGACAGAGATTAGCTGTAAAAGATATTTAGAACACTGAAACCAGGAGTTGGACTCTATGA
    TCCTTGTGTGTCCCTTCCATCTCAGGTTATCTTATGATCCAAGACCTTGGCTGGGTTTAGAGATGCACTT
    CACACTTAAAGTTTGTTGGACCATGTGAAAATGGTCACATTTACAGAGAAGTGAATGGCTTAGTCTCTGT
    GCTTGAGGCATTGAGGAAAGAACTGGGGAGAATTTACAATAAAAGAGGTAACATTTCTATGAAGTGAGCT
    GGAAAGCCTTTTCCATTTCTCTTCTGCGGCTTGGAGTTATGAAGAGTTTCTATAACATAAATGGAATTGA
    ATAATTGTCAACAGCTTAAAGAGAGATGAGGAAGGCACAAATATACACTTACCACAGGATCCATCTCTTA
    CTCTCACATTGTCCAGTGCCGTATCTGCATACTCTGTCAGTCCTCGGACAGCCTCAAAAATGACCTAGGC
    AGGATGCAAAGTCAATCGCTACATCAATTGTTCAGGCATATGTGAGAGAATTTCCCTCAACATGTTTTCA
    GAATACTCTTTCTGGTCTATTTCTAAGGGCATCCAGGGAACCAATGGTGGAACACTTTCCCCAGTATTCT
    TCAAGAAAAACTTGTAGAAGTTTTTTGTAGAAAAAGTAAGGCACCTAATGTCTTTGACATCATGATAAAA
    TGAGGGAAGGAGGGAATGTGGGAAGGAAGGAAAGAGAGAGCAAATCAAATATATTCTATCTCCTAGCTAA
    AAACACCAGTAAACCTCAGGGAGTTGTTTGTTTTTTCTCTTAGTTTATTTGCACAAAGCATGAGGGCATT
    ATAATTACAAAATAGAAATCAAGAAAATTTTGGTTATTGTTTCTAGCTTATACATACAACCTTTTACAGC
    CTGCAAAGGGAGAGCAAATCATAAATGCTGATGCTGTACAAACAAATTTATTATGAAAATATATTACCTT
    TATCTTTCTCTGTGTTGGAAAAGTATGTGTGACAGCACCATAAATCCAAGAGGAACTTTGGTTTCCCTTC
    CGACTCCATGCCGTTAACTCCCCTGTGCTGTCTTGGATAAGCACTCTCAGTTCATTCCTGTCTTCTGACC
    CAAACATATAGTACCAGAAGTCTATGCATATCTTTTCAGAAACCACAATATCTGGGCTCTCAAGCCTGTT
    AGTGTCGAATGGGATCAGATTGCTTGCTTCTTGGTAGATGAAATAGCCTTCTGCAGAGTGAAAAAAATAA
    AAAAAATAAGTCTGAATCTTTCTAACTTGTTAGGTGTCACTTAAAACTGAGGTACATCTCAACTCTGGAA
    GAAATCAAGCACATCATTAGCAGTACAAAGAAGCAAAGTAGCAAATGATTGTAATTTATACTGCAATGGT
    TAGCACTGTGTAGTTAGCTAAAGTACAATGAGACCTGGAGAGCAAAGCAAATATATCCTATCTCATATGA
    ACAGTTATTTTGGGATTGCAAATAAATATCTAGGCTTCTTTCAAAAGACAGCATTTCCAGACACAGTGCC
    CAACTTATCCTTGTTCAATTTCCACTTTCCTCTGAGGAAAATAATGTGATCTACTATGCATCTGCTATAC
    ACCTTCTCTTGCAAGATACGGTCTAATGATAAACAAATTTTTGGCATAAGGAAAATTCCAACCCCTCAAT
    ATTTGGTTTTGTCTCAAAGCAGAGGGGTAAGAAAAAAAACACACAGATATTTCCATTAAAATGTTAGGTT
    TGACATTATCAAAAGGAATCAATCTTTTCAAAAATCAGAATCTCACAATGTTTGATTTTGGGATTTAAAT
    ACTTTACTTTGAACTTGTTAATTGTGAGATCACATTTTCCTTTACTAAGGCTGGAGCTTTTTGCTTCCTA
    TCACTTCTCTCTGCTAAGATGAAAACTGTATTTTGCCAGTGCAGTTATGTCAAGTCTCTCCCATATGACT
    ATGGAAAGGAGTAAAAAAGAAATGACCATATCTACATTATAAAGCATTACTCCACACACAGTATCTAATA
    TTCAGTCTACTGTTTTAGGGCTCTTTTAATAATAAGAAAATGTAAGTAGCACTTGAAGTTATATGAACCA
    TGCCAATTGAAAAAAATATATACTTATTGTAAGTGACCTTTCCAGTCTAGGGATAATTACTGAAAACAAT
    ATGGCTACAGATTGATCTCCTATAAATGAATCTTCTAGTGCAAGGTTGAAGGTTCTACCATTCCATCTAG
    TGATGGAGCCATATTAAACACTCTTTTAATGATAGGACCACATTAGACCTCAGTTTTTCAATCCCATGTA
    GCTGAGGACGAGTTTTAAATGTATTCTTTGATTTCTGACTGTAATTTCCAATCTTGTAATGCTTTGCTAG
    CATGAACATTATCCAGTGTCAGCACAGTGATCATTACATTTCTTGGCGTCAGCTGATATCACTGTAATTT
    GATTATAAAACTTCTCCTGTGTAATGATCAATTGAATGTCACTGCATTCTCTGTGAGGTCTTAAGCAAGA
    TGTAGTTAGCATGCTTCTACTTGCTTTTACTGCACTGATAAATATTTCCAACCTATGATGGGGAAAGCCA
    GTTTAATCTTGTGACTGAACACGGTGAAAAGTCAAAGACAGGTGTCAACCAAACCGACACATTTCAAAGG
    ATTTCATTAATCTCTTCATGAAAAAGAAAGAAGAAATGTAGACAAGCTGTATTCAGTAAACAATGAAGTA
    AACAATGCCATCAACAAGCACTTACTTCCATCAGGATAGTCTCCATCAGGACCTGTTCCATCTGTTGGAG
    TCTCATGCTTGGTTCGTATCCATACGCCCTGGTTCACAGTGCAAGGTTGGGTCCAGTCACAAAATGGTCT
    TGAGTTGTTGTTAAAATCACATCGAGTGAGGTAAGCTGAAATGCAGAATAAGGTAAGTAAGGAAAAGGAG
    ACACTATATTCAAAACCTGCCTTGGACATTAACAGCACTAAGTATAAACAGTTACCTGTTGCAGGATCCC
    ATGATCTCAAGCCACCGGTTGGTCTGTGTTTGATCCTGCCAAGAAAAAGAACACAGTGATATCCCTACAG
    ATTTAGTGACACAAAAACTGTTTAAGAGTGTAGAGACTAACAGAAGTCATCAGGTGTTCTTAATGAATCA
    CTGCCCAGACTCAGGGTCTCATCTGTGTCTGACTCCTTCCCTTCCCATCTGTCCTTACTCCTTCATTAAG
    AAGTTCTGAGAGCTAGAGAAAAAGTGTGATATGTTCACCTCATCTGAGTATAGAGGGATCCTCTCCCTGT
    ATCTCTCTAAAGTGAAGGGAGAAAGTTCTACTAGGCACAGGGAGCAACAAGAGAGATGGCAAGGTACAGC
    AAAGTTCGGATAATACCAGAATAACACAAAAGACTTGCTATTAACTTGTTTGTAGCACTGGCATCCTCAC
    TACTGCTAAGCAAGATACGTAAACGCCAGTAAACTGGTGTGTCACAAAACTGTTATTTGTCAGAAGGCTT
    ATGTTTCCTTTAACCACAACAAATATCTCATGACCACAAGCTGCCATCTCATTTAGTTTCTCCTGTAAGT
    TATCCTAAAAATGCTACAGGGCAAGGGCAGTTAGAAATAGATATAGTCCCTTTGGTGCTAGAAAATCCTG
    CAGAGGTGAGATGACATAAAACCAAAGCTCTGTCGCAGATCATAAGATGGCTCTATTCATTGGGTGTTTT
    GCATCAGAAAGGGATTTCCAGGACTGCTAGACTGCAGTAGCTGCTCTTGACATGTTCTGATGGTGGTCTA
    CAGCTTTAGGAGGCAGGGTCCAGTTCCTATCCCTGTGTTGCCCACCATCCCTACTGTCCTTGTTGTACCT
    GACACAGTATAGAGAGAATCTGTCCTTAAGCAAAGCCATCACAGCAGATGTCCCCACAGCCCATGAAAAT
    CTAAGCAGAGAGATTCAGAAAAACTAGGGGGATTCATAGTATCATATACAAACAGGATCTACTTCATCAC
    TGAAACACACCAAACAACTGGAAAACAGACGAAGCTAAAGCTAAGGAGGAGAGAGGTGTGAAGATAGGTG
    AAGTGAGAACATTTTCTTCTTTCCCCACAAGACAGCTGTAACAAGATGCCATTTAATCTCTATTCTATGC
    TATCTTTAAACCGCTATATATCAAAGTCCTAAGGCAAAGGTTATTGTAGATATACTTAGAAAGTCACAAA
    CATTTGATAACTGCAGCAGATCTCCCCTTTGGTTCAGCCATTAGAAATGTACAGTTTACACATTCTCTCA
    CAGACAGCAACCACTGCAAAACTTGGCAATAACACAATTCAATTATTCCCTCCTTTTAAAAATAACATGG
    TTTAATCGTACAGCATTCATGTGTAAAAAGATGAGAAAGGACATTTATAATTAACAATAACTACGTTTCC
    TGAACTAAGGTAGATTCAGCATTGTGCTACTTACCCTGTGGTATTCAGCACCAAAATACAGAATAAAATC
    AAAATGCATTGTAAGTGCCAGAGGGTCCCCATGCTAGAAGACAGTCAAGTCTTCTGTGTGGTACAGTCTA
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Gallinarum relA/spoT mutant vaccine
7. IgG2b
  • Gene Name : IgG2b
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16016
  • Genbank Accession : AC160982
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 56717
  • Gene Ending Position : 60336
  • Gene Strand (Orientation) : -
  • Protein Name : immunoglobulin heavy constant gamma 2B
  • Protein Note : Also known as IgG2b; Igh-3; gamma2b
  • DNA Sequence : Show Sequence
    >gi|121699722:56717-60336 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
    CCTAGGCTCCCTGCCCAATCATGTTTCTGTAGTCAGGGGAGATCTTCTGCTTCAGCTCCACCACTGAGGA
    GAAGATCCACTTTACCTGTGGGGAAGTGAGAGTCAGAGACCACACCCATGCTCAGACCCTCCAATCCTGT
    GGGGAAGACAGAGGCCTGTCTCTTCTTCAATTCCTTTCATACAGGTAGGAGACTGGGTATGGGAAGTGGT
    CAAAGTGGAGATATTGTGGGAGTTGAGGTGGAGATGGTATGAGATTAGCTCACAGAGTACCTTGAGTTAT
    GGTGAGGCGAACATCAAGGGGATTGTGTGTTAGACAGCATGAACCTCATTAAGGCTAGTGTGGAATGCAA
    GGAGGTTTGGTCTCATAAGACAGTATGAGGATCACGCAGGACAGCAGTAGAGACAGAAGGGCAGACTGTA
    TGGGACAAAATGTGGAGTCACATAGAGCAATGTGGGATGGCTCAGGGAATGGAGGAAACGGTGTGTGGTA
    CAGAGTGGGGACAGCAAAGTAACATCCTACTACATCAGGGGAGGAGTGAGAGGACATTTTGTGGTCAGTG
    TAGCTATCACAGCAGAGGGTGGGAGACAGTGCCAACCTTGAAGAGTGTGACAGAGGCGCTGTAGCACACG
    CTGAGCAGGAAGAGGCTGATGAAGATGGTGATGGTCGTCCAGAGCCCGTCCAGCTCCCCATCCTTGGCCT
    CAGCACAGATATCATCCAGGTCTAGCCCTGAACAGAGAGGACAGTCAGTGTTTGTCCCAACATGGTTGAT
    GCTGGGAGAGATTCCCTGTGAGGCAGACATGTCTGCATGAAGTCAGAGGAGAGATCTCCAGCCTGACTGG
    GACAGCAGACCCTCCCCGGGGCCTGCTTTCCTTCCTTTCCCTCTGGGACATCAGTCTCAGCTTCCTCCAC
    CTGAGTCCAATAATGCCTTGGATGGACCTCAGAGTGACCTGGATTATTCAAGTGCCCTCTATGCTTGTCT
    CATCTTTCATTCCTTCAGTACAGGAGAAGGTGGAGGGTCAGCAGGGCCTGTGACCCAGGTCAACAGTGGC
    TATACAGCTGGACTGACCCTTTGCTATTCTTCAGTCATCAGCAGAGCCTGTGGCTTTTCTTGCCTGAACT
    TGGGAACATCCCAGTGTGAAGTGATTCAGACCATTATGGCTATGGTTTGAATTATTCATGTGGGGTTGAA
    GCATGACCCCGAGGTACCAGAGATGAATCTGGCTTCAGAGGTCTTGTTTTGAGCGGAGAATGGAGTGTCC
    TCAGAAGAGGAAAGGTGTGTCTGATTGTGATGGCAGAAACGTGGGCATTGAATTTTTGGCCGTGTGTATG
    GTGATACATTGCTGGATGTAGGTGCTGTGCTGGGCACAGAGGAGTTGTCCCAGTGTGTTAAATGCGTTTT
    GTGCTCACGAGCATGAGGATACATGTGCTATATGGACTGGATGGTGTTCAGCAGGGTCAGGATTTAGCAT
    GAGTGTGGCTATCTAATAGTCCCTCCCTTGGGGCATCAAGAGCCATGTGCCTCAGCCCCAGGTGGCACAG
    GCAGGAGTGAGCCATTTGTATGGGTGTGCAGACCTCGTCTGTGAAAAGCTGCCTGAGTTGAGAGTATAGA
    TGACAAAGGTCTATGGAATGCCCCTGGCCATGTGTCACTCCACAATAGACTCAGAAAAACCCCTAAGTAG
    AGAAATGGGGCAGGGGGACAGGAACAGCCTAAGGGAGGTGGGTTTGTGTGCTTATCCATTTGAGGCTTCC
    TAGGATTATCTCTATGTCTGGCAGCTGCTGGTTCATGGGAAGGCAAGGAAAAGGCACTGCCTAAAGCCAA
    GCCCCAACAGTCCAAGGCAGAATGACTCATGACCTCTGAGTCATGCAGTGCAGAAACCTCATTCTCTTCC
    TATAGACAAGGCTGTCCTCCAACCCTGGCCAGACCTATCAGCCCGTGAGCTATGCTCTATACCTTGGAAA
    GAACCAGGACAGTTTTACATGGTACCAGGCTTTGCTGGATGCTTTATTTATACAAGGGATGCATGGATAT
    GGGTGCCAGTGTCTCTTAGGACCTGAGAGCTTTGTGGGTGCTGAGCTCATTTACCCGGAGACCGGGAGAT
    GGTCTTCTTCAGGTAGTAATTTTTCAGACCCTCGTGTCTCACGTTGCATGAGAAGGAATCTGTTTTCTCC
    CACTTGCTTGTTTTTATATCGAGCTTGCTGTATATGAAGTAAGAACCGTCAGAGTCCAGGACTGGTGCGG
    TGTCCTTGTAGTTCTCCTCTGTATGCCCATTGCTGGTCCACTCCACACTGATGTCTCCAGGGTTGAAGCC
    CACGACCAGGCAAGTGAGACTGACATCTTTCCTGGACAACTGCTCTGCTGGTGGCGGCAAGATGTATACT
    TGTGGAGCTCTGACTAGCCCTGTGGGGTTAGAAACATACATAGAGGTCATGGCTGAAGTGGAAGGCTGTC
    CACATAGACATTTATTCTTATGCCCATCCCAGCCCCCATGCAGTTGTCCTGCAGGTCCCACCTTTAATTT
    TTGAGATGGTTCTCTCGATGGGTGATGGGAGGTCTTTGTTGTTGACCTTGCATTTGAACTCCTTGCCACT
    CATCCAGTCCTGGTGCTGGATGGGGAGGGCACTGACCACCCGGATAGTACTGTTGTAATCCTCTCTATGG
    GTTTGTGTCTGAGCTGTGTGTACTTCCACGTTGTTCACAAACCAGCTGATCCGGACGTCTGGGTCATCCT
    CGCTCACATCCACCACCACACACGTGACCTTGGGTGTCAGGGAGATCATGAGTACATCCTTGATATTTGG
    AGGGAAGATGAAGACGGATGGTCCACCCTCGAGGTTAGGAGCTGATGAGAAGAGATGGAAATGGATTTGT
    ACATGGCTTATCCTCCATTACAGGGATTTTTACAGCACTTACCATTCTCCTGGGAGTGGAGCTCTGGTAG
    TGACTTACCTGGGCATTTGTGACACTCCTTGCATGGAGGACAGGGGTTGATTGTTGAAATGGGCCCGCTG
    GGCTCTGCAGAGAGAAGGCTGGATGTTACTGGTTTTTGAAGGAGAGAAGCACCAAACCAGAGAGAGGAAA
    GTTTGTCCCTGGTATGGGCTTAGTCCAGGATGATCCTGGAAATGACAAGCACACCTATCCCAGAGGATCA
    TGTCCTTGAGTGTAGGTGGTCATAGCTAGATGTTCCCAGGAGGGAGGCAGAGACAGTATCTCCCTCTTCC
    CTGGGCTAAGGTCACCTCCTTGGTGATGGATCCCAGCCTGGCTGGTTTAGATAGGCTAATACCTTTGCCT
    CAACTCTGGTGAATCCCTCCTCCCCTGAATGTCCTCTCACCAAGTTTTTTGTCCACCGTGGTGCTGCTGG
    CTGGGTGAGCAACGCTGCAGGTGACGGTCTGACTTGGCCAGGTGCTGGAGGGGACAGTCACTGAGCTGCT
    CATAGTGTAGAGTCCAGACTGCAGGAGAGCTGGGAAGGTGTGCACACTGCTGGACAGGGATCCAGAGTTC
    CAAGTCACAGTCACTGACTCAGGGAAGTAGCCCTTGACCAGGCATCCCAGAGTCACAGAGGAACCAGTTG
    TATCTCCACACCCAGGGGCCAGTGGATAGACTGATGGGGGTGTTGTTTTG
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Typhi phoP mutant vaccine
8. Ighg1
9. Ighv1-9
  • Gene Name : Ighv1-9
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 668478
  • Genbank Accession : AC073561
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 114583568
  • Gene Ending Position : 114583861
  • Protein Name : immunoglobulin heavy variable V1-9
  • Protein Note : Also known as Igg2a; Gm16697
  • DNA Sequence : Show Sequence
    >gi|372099098:114583568-114583861 Mus musculus strain C57BL/6J chromosome 12, GRCm38 C57BL/6J
    GTCTTGCACAGTAATAGATGGCAGAGTCCTCAGTTGTCAGGCTGCTGAGTTGCATGTAGGCTGTGTTGGA
    GGATGTATCTGCAGTGAATGTGGCCTTGCCCTTGAACTTCTCATTGTAGTTAGTACTACCACTTCCAGGT
    AAAATCTCTCCAATCCACTCAAGGCCATGTCCAGGCCTCTGCTTTACCCACTCTATCCAGTAGCCAGTGA
    ATGTGTAGCCAGTAGCCTTGCAGGAAAGCTTCACTGAGGCCCCAGGCTTCATCAGCTCAGCTCCAGACTG
    CTGCAGCTGAACCT
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Typhi phoP mutant vaccine , Salmonella Typhimurium asd mutant vaccine , Salmonella Typhimurium asd/rfc mutant vaccine
10. IgM
  • Gene Name : IgM
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16019
  • Genbank Accession : AC073553
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 171229
  • Gene Ending Position : 175133
  • Gene Strand (Orientation) : -
  • Protein Name : immunoglobulin heavy constant mu
  • Protein Note : Also known as Igm; muH; Igh6; Igh-6; Igh-M; AI326478; MGC107036; MGC107507
  • DNA Sequence : Show Sequence
    >gi|121699722:171229-175133 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
    GTCATTTCACCTGCAGGTGACAGAGACAGTGTGAATTGCTGTCCACTTGTAGGGAGTAGAGGTAGAGGCA
    GTGAGGACTGGTGACTCCCTGTCCCAGCCCTGTGTCCTCAGCCCCACAACCACACTACCTTGAACAGGGT
    GACGGTGGTGCTGTAGAAGAGGCTCAGGAGGAAGAGGACGATGAAGGTGGAGGCAGTGGTCCACAGGTTC
    TCAAAGCCTTCCTCCTCAGCATTCACCTCCCCCTCTATGAAGGGACACAGAGAGGGAGGGTCAAGCCAAG
    TCTTGGGGTGACTGAGCATTTGCATAAGGGTTGGGGTGCCCTGATGGTTCTGGAGAATCTTAGCGAGTAT
    TGACTGGTCCCCTAATAGCTCAAGCTAGACGGAGGCACAGAACAGGCCAAGCTGTAGGACACACTAATTT
    GGAGCCTTTGTTCCAGGAGCCCTGGGGCAGCAGAGCAAGCAAGACAGATGCAATGTATGGACCTGGGTAA
    AGGCAGACTCAGGAACTGTGGCAAAATGATATTGGTGTGCTCTGGACTTTCCCTGAGCAGGTGGTGCCAT
    CGGTGCTGTTTAGACTGAGAATTTAGAGCAGGTCTCCCTGTCTCTTGGGCTTTAGCAATAAGAACAGGGA
    TACCAGAGGTTGTCCCTCCTTACTTCCTGAAGGACTCAGGCATCTCATGACTTTCTTTGATCAGAGTTCC
    CGTGCGTTGCTTGTCCTACCCCTGCCAGCAATGTTGGGTCTTGGTACCAAGAGATAAACAGACAGATACC
    TGAGGGAGGCTGTGGGACCAGGGCATTTATTGGGATCTCTGATCCCTGTAAAGATGAGCCCTTGGGGATA
    TCTAAAGCCTAGGCCCCAAATGGATATGAATATGAAGAATGTCCAGGATATGAGCCCAAAGAGGCAGTGT
    TATTGGGTGGTGACTCCTTACAGAGGCTCCTTGATGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
    GTGTGTGTGTGTGTGTGTGTGTGTGTGGTTGGGGCATCTTTTGGACAACTGCATTATGTTTTCTGCATTT
    TGAACTGGAGATCAGGGTGGGTGTGAGTGGGTTAGGCAATTTCACATTGGTCTTCTGAGGTGTTCTATTA
    ATGGAGACACAGCCAAGGCTAACTTTCTGTCATGGGTGGTTAGTGGAGTATCTTCTGCCCCATGGTGTTG
    AGACAGATAGGCATACTTTGTACCTGGGGTTATCTCTCCCAATCATAGAGTGGGCTCCTGAGGAGTATCA
    GTGACCCAGCCTAGTGTAGGCTCTCTGAGCCGTGAGCCTTGGAGATTACAGCCCTGGTCAGAGTCCAGGT
    GTATCTGTGTGCTATACCTGTTCACATGTTAGTGTACACATGTGAAGATGTTTCTGTACATGTGTGTATA
    TGCATATCTTTGTATCTATATAGTTTATTTGTGTATATTTGCCTATAGTCTGTTTCTGTGTACACAGGAC
    CATGCATATTGGTATCTGTTTCTGTGAGTGGTTGTGTGTCTATGCCTATTTGTCTCTGAAGGCGTTTGTA
    AGTGTCCATGCATGTGTGTGCGCATGAACTCATGTGTATATATGTGTACCTTTGTCTGTGTGTCCATGAA
    TACACATTCATGTATGTGTCTACACATGTGTACCTATATATCTATGTATAAATGCATGTGTGTCTATATA
    TGTGCCTGAATGCTGCCTGCACCAGGTTAGGCAAGCTTTCAGCCATATATCTAGAAGTAGCTGGAGGAAT
    CGCATGTTCATACTCTGTCTGTTGTAAGCCTGACTGAGTTCACACACAAGGAGGAAACTGGCAAGGTAGA
    GGAAGGGCCAGGGTGGAGGCAAGTATGCAGGGTGTGTGCAGAGCAAGCAAAACACAACTGTCTCAGCAGG
    CATGAGCATTGTATAATCAAAACCAGTGACGTTTGAATGGATTTTTTTTATTTCTAATTTTTATAAAATG
    CAACATCTCACTCTGACATGGTTAGTTTGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGA
    GCGCTAGCATGGTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTACAGTGTGGGT
    TTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGTGGCAGGGCCTCGTGGCCTACAACACAGG
    TATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGCTGTGGGTAAAGTAGAAGCCTGG
    GGCCCCAGGCTCTGGCATCGGGGCACTGGTCACATACTTCTCTTGGGGCAAGAGTTGCCCTCTCTGAAGC
    CACTGCACACTGATGTCTGCAGGAGAGAAGCCCTTCACCAGGCAGGTGACTGTGGCTGACTCCCTCAGGT
    TCAGTTGCTCACGAGCTGGTGGCAGCAGGTACACAGCAGGTGGATGTTTGTGCACCTCTGTAAATGAAGA
    CAGTAGTGAGGATAGGGTGGAAGAGGACCTGCCCTCCCTATGAGGTACAGGAAGGGTCCTGCAATTGGAG
    GGGAAGGGAAGGGGGGATACCTACCATTGGGTTTTGAGATGAATTTCTTCTGTGGTGAAGGCAGATCCCT
    GTGAGTCACAGTACACACAAATTCCTTCCTGTTATTCCAGTCTTCCACACAAACACTAGCCACACCCTTA
    GCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTTGGTTTCCAGTGGTTCACCACTTTGAG
    AAGCCCAGGAGATATTCAGGGTTTCATAGGTTGCCAGGTTTGAGACCAGACAGGTCAGGTTAGCGGACTT
    GCTGAGGAAGATGTCGGCAAAGGAGGGGGGGATGGTGAAGGTTAGGATGTCTGTGGAGGGACCTGCAGTC
    AAGAGAACACTGTGTCAGGTTATGACATATCTAGTTGTGAAGTTGGAAAAAAGCTTAGTCTCCATCCCCC
    AAACCTACTTAAGGGAAGATGCTGGTCCTTCCAGCTGATTCTCACCCCTCTACTGCCTATTGCTCATTCT
    TGTCCTTGGCCACTCTGGGGAGCCAAAGTTCAAGGAGCAAATGACCATGTCTGGGTGGCATTGGCCATAA
    TTGTAGGAGGACTTCCCTAATCTGGGAGGGCTAGGCATTGGGCTTAGCCCAGGCCACTCACTGGCAGCAC
    ATGTGGAGGACACGTTCTTCAAGAAGGTGAGACCCCTGTGATCCACACGGCAGGTGTACACATTCAGGTT
    CAGCCAGTCGATTTCAGAGATGGTAAGTGTGCTTATGACCTTGTAGGTTTGGGGTGTGGATCCTTTGTTC
    TCGATGGTCACCGGATCTGTGGTGAAGCCAGATTCCACGAGCTTCCCATCCTTTAGCCAGGATACTGTGA
    TCGGTTTTGGAGTGAAGTTCGTGGCCTCGCAGATGAGTTTAGACTTGCGTGGTGCAGGGCCAGAGAAGCC
    ATCCCGTGGTGGGACGAACACATTTACATTGGGGTTCATCTCTGCGACAGCTGGAATGAAAGGTCAAGGT
    GTGAGCTCAGCTTGGCCTAAGCCCCACCCCGCTGCTCCCTCTGGGCCATGCCTGGGCCTGGGCACCCCTG
    CTGGGAGGGTTTGGTTCTTACCTGGAATGGGCACATGCAGATCTTTGTTTTTGCCTCCGTAGTGGATTTT
    GCATACCAGGTATTCATCTGAACCTTCAAGGATGCTCTTGGGAGACAGCAACACCTGCGAGGTGGCTAGG
    TACTTGCCCCCTGTCCTCAGTGTTGGGAAGGTTCTGATACCCTGGATGACTTCAGTGTTGTTCTGGTAGT
    TCCAGGTGAAGGAAATGGTGCTGGGCAGGAAGTCCCGGGCCAGGCAGCCCATGGCCACCAGATTCTTATC
    AGACAGGGGGCTCTCGCAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTC
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Typhimurium poxA mutant vaccine
11. IgM
  • Gene Name : IgM
  • Sequence Strain (Species/Organism) : Sus scrofa
  • NCBI Protein GI : 164513
  • Other Database IDs : CDD:209398
    CDD:143166
  • Taxonomy ID : 9823
  • Gene Strand (Orientation) : ?
  • Protein Name : immunoglobulin mu chain
  • Protein Length : 403
  • Protein Note : Immunoglobulin domain; cl11960
  • Protein Sequence : Show Sequence
    >gi|164513|gb|AAA51297.1| immunoglobulin mu chain, partial [Sus scrofa]
    QNIQDFPSVLRGGKYLASSRVLLPSVSIPQDPEAFLVCEVQHPSGTKSVSISGPVVEEQPPVLNIFVPTR
    ESFSSTPQRTSKLICQASDFSPKQISMAWFRDGKRVVSGVSTAPVETLQSSPVTYRLHSMLTVTESEWLS
    QSVFTCQVEHKGLNYEKNASSLCTSNPNSPITVFAIAPSFAGIFLTKSAKLSCLVTGLVTRESLNISWTR
    QDGEVLKTSIVFSEIYANRAFGQGEASVCVEDWESGDRFTCTVTHTDLPSPLKQSVSKPRGIARHMPSVY
    VLPPAPEELSLQEWASVTCLVKGFSPADVFVQWLQKGEPVSADKYVTSAPVPEPEPKAPASYFVQSVLTV
    SAKDWSDGETYTCVVGHEALPHTVTERTVDKSTGKPTLYNVSLVLSDTASTCY
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Typhimurium gyrA/cpxA/rpoB mutant vaccine
12. IgM Fc receptor
  • Gene Name : IgM Fc receptor
  • Sequence Strain (Species/Organism) : Gallus gallus
  • NCBI Gene ID : 771853
  • Taxonomy ID : 9031
  • Chromosome No : 26
  • Gene Starting Position : 2406653
  • Gene Ending Position : 2408482
  • Gene Strand (Orientation) : -
  • Protein Name : Fc receptor, IgA, IgM, high affinity
  • DNA Sequence : Show Sequence
    >gi|118135660:2406653-2408482 Gallus gallus chromosome 26, reference assembly (based on Gallus_gallus-2.1)
    ATTAATACTTGGCTAACATTCCTACTTCAGCTATCAGGGACAGAAATGATGAGGGTGAAGAGTGAATTGC
    ACCTGTGTCTCTGCATGCTGAGGTTCCTAGAACTGCAAGATAACCTTCAGAGCAAAGCACATCTCCATGC
    TGCATGTGTTGTGCTCAGGATGCCATGTGCTGGTGTTTTCTATCATAAATTGTCCATGATATTTTCAAAC
    AAACTTGAGCAGAAAGCTCAAATGTTTCTTGTAAAGCCACTACATTTATGCATGGGACAAAGGAGAGAAT
    TTGAAGGGGGTATCCATGAAGGGATAGGGGGAAGAGTGATTAACAGAAGGAAAAGGGTTGATTTGTGTTT
    CCAGCCAGCCATCGCCTACCCGTCTCGATGCGCAGTTTCATGGAGGTCGTGTGCTCCCTGTGCCTGTTCC
    TGGCCACACACCAGTACCACCCCGCGTCCTCCTCCCGCAGGTGGCTCATCACCACGGTGTAGGTCCCACT
    GTCCTGCTCACTGTTGCTGATCCATATGCGCCCTTTGTAGGACTCGTGCACAAACCCGTCTGTGTCTACC
    AGCAGGGAGCAGCTGGCCTCCTGCCACCTGCACAGGTACTTGGTGGTGTAGCTGCCCACGGGGTTGTGGT
    GGCACCTAAGGGACAGTGAGCCCCCCAGCCTGCCATGCAGAAACTTCAACCTCCGGGGCTGGGCAGGGAC
    TGCAGGTAGAAGAAATCATGGTTATGTTTGATGTAGCTGCAATGAGCCAATGGGGCACAGCAGCCCCCAA
    AACCCCCTCCTACCTGTGGTCACCTGCAGGGCCACCAGCTGCCAGCTGTCCCAGTCAGCTGGGCTGCCCA
    TCCCACACTTGTACAGCCCTGCATCGTCCAACCTTAAATCATTTATCAGCACTTTGAATGCTCCAGAGCT
    CTCCTGGGGGGTAATACAGATCCGTCCCTCGTAGCTCTTCCCCACATAGCCATCGGTGTCAGCAATGAGG
    ATGCACCTGCTTCTCCCCACTCTGCACCATAGCCTCCTGGTGCCACCCCAGCTGTCCCCGGGTGGGCAGG
    GGATGGTGATGGAGCCACGGAGCTCCCCGAGGTGCAGCTCAATTGGCCTCTGAGTGCCCACATCTGCAAA
    GACAGAGCAGGTGAGTGCCACCACTGTGCCTGGTTGGGTGTCACCTCCTTTTGCATTGGTGCATTGGTCA
    CATCAGTGGGTTTTATGCCCACGACTGCTTAAAGCAAAGGGTAGCAAAGGGGACCTGACACCCTCCCTGT
    CCCATCACTGTACCTGCTGACACTGTCAGGTTCAGGCTGACGTAGAGGTCCCTGTTGGTGGGGCCAATGC
    CACAGCGGTAGGTCCCTGTGTCACTTGTCTTCAGCTGTGTCATTGTAACCATGAAAGTGCCATGCTGGGG
    GACGTCCTCCAGGGCCACTCGGCCCATGTGGTCTTTTGAGATGAAGTTGGTGGTGGAGATGATGGTGTAG
    CAAATCCCATCTCTGTCCCTTTTACACCAAAACTTTCTGTCGTATTTGTTGGCAGGCGTGATGGAGTAGA
    AGCACTGGTGGGTGATGGACCCTCCAACTGCGCCTGTCAAAAACCTGGGCCCATACAAGGTGCTTGAGGC
    TGCCTCTTTGGGGAAGGAGGGAAGAAGCACATTAATCAGAGACGTGGACACCCCTGTGGATGGGGAATCT
    TCCCCAACCCAAATGGGGTGCCACAGGCACAGGTCCACCAGCCCTGGCTGTCCTGCCCCATAGTGATGGG
    GAGCAGCAAACCTTTGGTGCCTGCAGAGTTTTCTGGTGACTCACCTTGCAGCAAAGACACAAAGAGGAGG
    AGAACCTCCA
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Gallinarum relA/spoT mutant vaccine
13. IL-17
  • Gene Name : IL-17
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16171
  • NCBI Protein GI : 6754324
  • Genbank Accession : AC159614
  • Protein Accession : NP_034682
  • Taxonomy ID : 10090
  • Chromosome No : 1
  • Gene Starting Position : 20730904
  • Gene Ending Position : 20734495
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 17A
  • Protein pI : 9.13
  • Protein Weight : 16001.99
  • Protein Length : 158
  • Protein Note : Also known as Il17; Ctla8; IL-17; Ctla-8; IL-17A
  • DNA Sequence : Show Sequence
    >gi|372099109:20730904-20734495 Mus musculus strain C57BL/6J chromosome 1, GRCm38 C57BL/6J
    CATCCACCTCACACGAGGCACAAGTGCACCCAGCACCAGCTGATCAGGACGCGCAAACATGAGTCCAGGG
    AGAGCTTCATCTGTGGTGAGTCCTGCACTAATGTACAAGGCGCTTGTTGATTGTGACCAGGTTGCGTTAT
    TGCACGTGATATGAAATGCTCTATTCCAGCTGTTGGGGTCCATGAATCTGTACATGTAGAACTGGAAATG
    AAACCTTTGGTAGATGTCGAAATCATTGTACAGCATTTTCAAGAACTGATAGGCATCAGTCAGAAAGATA
    CGGTTAAAACTGAAGGGTTGCTGTGATGAAACTAACCCACTAAGAAATGTCACATGACGCTATGCAATGA
    GAAAGACTGTCAAAATCTATTCTTCTGAGTGCAAGGGATTTGACGTCATTCTCTCATGTCAGTGCTCTTT
    AATCCAGAATGAAGTGGGCTACTGTTCTTGCAGATTTGAAAGCTTTAGAAGCAAGTTTTCATCCATTGCT
    TTATCCCAAAGTCTTAGGATATCCAAACTATTAGATACATGTATAAGTCTCCTTATGAATAAAGCTTTAT
    ATATTTTAAATACTTAACCTAAAAAATATGCTCTGCACTCGTATTCTCATGATAGAATCAATGCATGCTA
    AATTTAGAACCGGATAATATATACCTAAGAAAACATGAGGGAGGTTCAGTTTCTATCTGGAGGATAGCTG
    GTGTACAAACACTGTTTCTAATTCTAACATGAGTGCCGACAAACAACGGGTAGGTTTTCTGCAGTCCTCT
    CCTCCAAGGTACATGTTTGACTGTGCACGAGGTTTACAGAAGGAGTGACTAAGAACACTAGTGCATAAGG
    ACAAGAAAATCGGGGTCATTCAGCAGCCCACTGACTCATGAGCTAACAGGCTGAGGAGATGGCAGAAGCT
    GACATTTTTATTAGGACCACTGCTGTCTAAGACATCACATGATTGTCCTCAGTCACAAAAAGAATGTAAG
    AAACTGGTTAAAAATAAAATAAATTCTACATTTTTTAGCATGATTTTGGCCATCTTAAAGTTTTTAAATT
    CAGAATTACTTACATATTAGGAGAGATCTTTCCAAAATATCATATGTTTCCTACAGTTAGTTATATACTC
    ATCACAATGAGTTTGTCAGAAAGAAAAGCAGATACTAATCCAATCCCCCATCACCTTATCTTTCCCTTCT
    AGTCTCTGATGCTGTTGCTGCTGCTGAGCCTGGCGGCTACAGTGAAGGCAGCAGCGATCATCCCTCAAAG
    CTCAGCGTGTCCAAACACTGAGGCCAAGGACTTCCTCCAGAATGTGAAGGTCAACCTCAAAGTCTTTAAC
    TCCCTTGGCGCAAAAGTGAGCTCCAGAAGGCCCTCAGACTACCTCAACCGTTCCACGTCACCCTGGACTC
    TCCAGTACGTAAGAACTCCAGACAAAATAATCTGTGTCCTTCCATTCTCTGATGCACCATGTATACCCTA
    AACATGATTTTTCACTTTTTTCCTCTCAGTATCCTCAGAGACCTAGAAGGTCCATTGTGTTAAGCAAAGT
    TTCCTCTACAGAGTTCTTGGATAGAGCAGGGAATACTTCCCCCTAACCTGTTATAGTGGTCAATAATGAG
    AGAGATCAACCCAAATTATTGATAACCATGATTCTAGCATGAGAGCTCAAGTTGAATGCTGACTTTTTTG
    GAAATCAGGATGTTTATGTTTCAACCAGACCTACAATTAAAGAGACTTGATGAAAAATAATTAATAACTA
    ACTAGGTATTTTTGTGTCTGACCTGAAGAAGACATAGGAAAACAAAATTTGAATGGCTGGTAGAGGAAGC
    ATCCTGCTGGATATGTTTTCTCCTTTGTGAGTTGGATAGACTTGACCTCCATCCCAAGCCCTTTTTTACT
    TGACCTGTCTCTGGCATACACTATTTAGACCCCATAAAGGGCTCAGTACTTTGATCATTTGAGGTTATAT
    GAATAAATTTCAATGGCAAGGAACCTGTGGAGCCACCTGTCTATAGGATTTTTCTCCTTTTTCCAAAACC
    AAGGATAATTCAATCAATCAATTCAAAAATCTAAGTGAACTATTTATTCAGATATTGTAAAGGGACAAGA
    AAGAGATTATAAAGGGACTTTTGTTGTTGCTTCTAGCTTTTCATATAACTTCCGTCCCAGGAAGTGTTCT
    CAGATATAATTCTGATATTCCTTCTTTCAAAGACCAATGTCACCAAATCAGATGAACAGCCATTTTACTC
    CTTGGTCATCTGTCCCTTTGTTGTCTCCTGTCAATCAAATGTACCCTTAAAATTTATCTTTTTTTTTTTT
    ATGTTATCTACTTGATGTTAAGTTTCAATCCTCACCTCACTATGGTCTTTAGCTTATTCTGTTTATCTTT
    CAAAAAGCATTCTTCTAAATCATTTCCTAAACTTTAAAATTCAAGTTTGTGAAGCTTCAGGTCCAGTTCC
    ATGATGGAATGGGTGTCCATCATGTGGAAGACACTGGCTTTGATCCTTATTGCTGCGAAAGAGGAGGAGG
    GAGAAAATGAGGAATCCGGGAGGAAAAGATAGAAAGGGGAGAATCTAAATTTTCAGACAAGCCAAAGTTA
    GCAACTAACATGCAGAGCTTTGCAGTGCACATGTGTGTCACAGTGCGTTCTTCCTCCCTCCCCCGTTCCT
    GCTTTTCCTTCCACTTAAGCAGGACTTCATTTCCTCCTGGCTTTTGTCTCCCCTGCAGCCGCAATGAAGA
    CCCTGATAGATATCCCTCTGTGATCTGGGAAGCTCAGTGCCGCCACCAGCGCTGTGTCAATGCGGAGGGA
    AAGCTGGACCACCACATGAATTCTGTTCTCATCCAGCAAGAGATCCTGGTCCTGAAGAGGGAGCCTGAGA
    GCTGCCCCTTCACTTTCAGGGTCGAGAAGATGCTGGTGGGTGTGGGCTGCACCTGCGTGGCCTCGATTGT
    CCGCCAGGCAGCCTAAACAGAGACCCGCGGCTGACCCCTAAGAAACCCCCACGTTTCTCAGCAAACTTAC
    TTGCATTTTTAAAACAGTTCGTGCTATTGATTTTCAGCAAGGAATGTGGATTCAGAGGCAGATTCAGAAT
    TGTCTGCCCTCCACAATGAAAAGAAGGTGTAAAGGGGTCCCAAACTGCTTCGTGTTTGTTTTTCTGTGGA
    CTTTAAATTATTTGTGTATTTACAATATCCCAAGATAGCTTTGAAGCGTAACTTATTTTAATGAAGTATC
    TACATTATTATTATGTTTCTTTCTGAAGAAGACAAAATTCAAGACTCAGAAATTTTATTATTTAAAAGGT
    AAAGCCTATATTTATATGAGCTATTTATGAATCTATTTATTTTTCTTCAGTATTTGAAGTATTAAGAACA
    TGATTTTCAGATCTACCTAGGGAAGTCCTAAGTAAGATTAAATATTAATGGAAATTTCAGCTTTACTATT
    TGTTTATTTAAGGTTCTCTCCTCTGAATGGGGTGAAAACCAAACTTAGTTTTATGTTTAATAACTTTTTA
    AATTATTGAAGATTCAAAAAATTGGATAATTTAGCTCCCTACTCTGTTTTAAAAAAAAATTGTAACAATA
    TCACTGTAATAATAAAGTTTTG
  • Protein Sequence : Show Sequence
    >gi|6754324|ref|NP_034682.1| interleukin-17A precursor [Mus musculus]
    MSPGRASSVSLMLLLLLSLAATVKAAAIIPQSSACPNTEAKDFLQNVKVNLKVFNSLGAKVSSRRPSDYL
    NRSTSPWTLHRNEDPDRYPSVIWEAQCRHQRCVNAEGKLDHHMNSVLIQQEILVLKREPESCPFTFRVEK
    MLVGVGCTCVASIVRQAA
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Typhimurium asd mutant vaccine , Salmonella Typhimurium asd/rfc mutant vaccine
14. IL-6
  • Gene Name : IL-6
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16193
  • NCBI Protein GI : 13624311
  • Genbank Accession : AC112933
  • Protein Accession : NP_112445
  • Taxonomy ID : 10090
  • Chromosome No : 5
  • Gene Starting Position : 30013160
  • Gene Ending Position : 30019967
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 6
  • Protein pI : 7.5
  • Protein Weight : 23291.86
  • Protein Length : 211
  • Protein Note : Also known as Il-6
  • DNA Sequence : Show Sequence
    >gi|372099105:30013160-30019967 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
    ACCAAGAACGATAGTCAATTCCAGAAACCGCTATGAAGTTCCTCTCTGCAAGTAAGTGAAGGCAGTTCCT
    TGCCCTCTGGCGGAGCTATTGAGACTGTGAGAGAGGAGTGTGAGGCAGAGAGCCAGCATTGTGGGTTGGC
    CAGCAGCCATCAGCTAGCAGCAGGCGCCCAACTGTGCTATCTGCTCACTTGCCGGTTTTCCCTTTTCTCC
    ACGCAGGAGACTTCCATCCAGTTGCCTTCTTGGGACTGATGCTGGTGACAACCACGGCCTTCCCTACTTC
    ACAAGTCCGGAGAGGAGACTTCACAGAGGATACCACTCCCAACAGACCTGTCTATACCACTTCACAAGTC
    GGAGGCTTAATTACACATGTTCTCTGGGAAATCGTGGAAATGAGAAAAGAGGTGGGTAGGCTGTGAAACT
    GATGAAGACCCAGTGTGGGCGTCCATTCATTCTCTTTGCTCTTGAATTAGAAATTCTCTGCTGGGATCTA
    GGGCCCTTAGGATTTGAAGCTAAAGGTCAGACTAGACTGTGTTCTCTCTTTCTCACCTCTTTGCTGGTTT
    TGAGTGGAGGTTGGGAAGGGGTTCCTTTCCTGTCTGGAAGATACAGAATGTGACTGCATTTCTAGAAAAT
    TCACAGTGGGCCATTCTCTGTTCCAATTTAGCTATTGCTTCTTAGGTGGGGATTCCTTTTCCCTCATTAT
    TCCTTTTTCCTTATCTCTTTGCCTTCATTTATCCCTTGAAGTCTACAAGGACCAGAAAGTGCTTTTTGGC
    TGAATGTAGTAAGTTCTGTGTGTGTGAAATCAGGATGCTCTAGGGTCAGCCCAGATAAGATGCATATTGT
    ATAGAGCCCAATAAAGTGCTAACACACACACACACACACACACACACACACAACCTAGATTAGGCTGGGC
    ATGGTGGTGGATGCCTCCCAGCACTTGGGAGGCACACGCAGGAAGATCTCTGTGAGTTAGAGGCCAGCTT
    GGTCTACATATTGAGTTCCAGGATATCCAAGACTACATTGAGAGATCCTTTCTCAAAAAAAAAAAAAAAA
    AAAAAAAAACAACAACAACAAACAAACAAACAAACAAGAAAAAAAAAACCCAACCAACCCTACCTAGATT
    AAAATATCTCCCATCTTTAAATGAAAAGTATTTTGACTCTGCTTGACAGAAAGGAAAAAAAAAATCACAA
    ACAAGATTAATAGCCTAGGTCTGGGTGTGTAGAACCAACTTTGTAATCTTGGGAAAATCCTCAACTATTT
    AGTACTCTTTATCTGCATAATTATGGAGTTACAGATAATGGCACAGACCCTTCCAGATGGAATATTCTGT
    GACTTAAATTTATTTGCCCCAAGATATCATCAAAATTTCTGTCCTTTACCAAGTTCCTACTCTATGCTGG
    GCAGTTTTTATCGTTGAATCCTTGAGACAAATGTATAAGAGGGACATCAATAATTCTTTATTGAAACATA
    GGAAATACTTAGGAGAGTAACAAGCTTAAAGCCAAGGGACTGAGCCTAAGGGTGCATTTTTATTTATTGG
    GCTGCTTAGTAGGGTTCTAAGAAGGTATAGCTTCCCTTTTCTGTGTCCTGGCTATGAAAGAGCATGACTT
    GGCCCTCTAGTGGTGCTTGTTGTAAGAGGTGTTTAAGTGATTGCTCTGGTAATCTTTTGCAGTTGTGCAA
    TGGCAATTCTGATTGTATGAACAACGATGATGCACTTGCAGAAAACAATCTGAAACTTCCAGAGATACAA
    AGAAATGATGGATGCTACCAAACTGGATATAATCAGGTAGAAACTTGTCACGTTTACTTTCAACAATTCT
    TCAAGGTTCTCTCTTGCACGTGATACCTGCATTTGTACAGTCCCAGTCAGGCAACAAAGATGGGGGGATA
    CATCCAATACCACTTGGATGTAACTGGCCTGCAGTAGCTTGAATACAAAAGGAGATTGAGAGGTACCTCA
    GCAGCTGCTAGGGTGAGGGAAAGTTTACCAACCAGTGCTATAAATGTTCACTCAATCTATGTCCATCAGG
    AGCTAAATGTTTAAAGTCTCTATTCTCATCTTGGCTTTGAGGTGTTAGATGATTAAAAAAAAAACATTTT
    TTTTGTCTAAAAAAGTGGAAGAGTAGATATAAAACATGTTATGTAAACGTCATGAGGAAAGTAACTTACA
    GATTTAATTAAAAAGCAGTTTATTTAAACAGGTAAAGGCCCACTATGCTGGGATTCTTCACCACTGTTAA
    ATGTTAGAAGTCATAGCAAGTTTAGCTAGTTCATGAGCCATCTCTTCTCTGGCTGCCCCTGGAAGGATCC
    AGGCCTACACTCCCTACTGAGAACACTCTTCACACCCCTCTCCTTCCAGCCGGTGAGATAGTCTACATCA
    GGTCTCAGGACAAGTCAGACAAGAACAAGAGTACAGGAAGGGCACTGCAGGATAGAAGGGAAGGAGTGAG
    GGTCAGAAGTGGATTCCCACTGCCACACTCTGGGGTAGGGGGTGGGGGGATATTAAGTGGTGCCTCTCTC
    TACTTGTCCAGATAATTCAGGAGGTCAGCTGATGCTGCCTGTTGTCTAATTCTGTAGAAGATACTGAACA
    GCAGGAATCTTTTTTTCAATTTTTTATTAGATATTTTCTTCATTTACATTTCAAATGCTATCCCCACAGT
    CCCCTATACCCTCCCCCCCCTCCCCGCTCCCTTACCCACCAACTCCCACTTCTTGGCCCTGGCATTCCCC
    TGTACTGGGGCATATAAAGTTTGCAAGACCAAGGGGCCTCTATTCCCAATGATGGCTGACTAGGCCATCT
    TCTGCTACATATGCAGCTAGAGATATGAGCTCTGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTAT
    AGGGTTGCAGACCCCTTCAGCTCCTTGGGTACTTTCTCTAGCTCCTCCATTGGGGGCCCTGTGTTCCATC
    CAATAGAAGACTGTGAGCATCCACTTTTTGCCAGGCATTGGCCTAGCCTCACACGAGACAGCTATATCAG
    GGTCCTTTCAGCAAAATCTTGCTGGTATATGCAATACTGTCTGGGTTTGGTGGCTGATTATGGGATGGAT
    CCCTAGGTGGGGTAGTCTCTGGATCTTCCATCCTTTTGTCTTAGCTCCAAACTTTGTCTCTGTAACTCCT
    TCCATGGGTATTTTGTTCCCCATTCTAAGGAGGAATGAAGTATCCACATGTTGGTCTTCCTTCTTCTTGA
    TTTTCTTGTGTTTTGGAAATTGTACCTTGGGTATTCTAAGTTTCTGGGCTAATATCCACTTATCAGTGAG
    TGCATATCAAGTGACTTCTTTTGTGATTGGGTTACCTCACTAAGGATCATACTCTCCAGATACATCCATT
    TGACCAAGAATTTCATAAATCCATTGTTTTTAATAGCTGAATAGTACTCCATTTGTAAATGTACCACATT
    TTCTGTATCCATTCCTCTGTTGAGGGACATCTGGGTTCTTTCCAGCTTCTGGCTATTATGAATAAGGCTG
    CTATGAACATAGTGGAGCATATGTCCTTATTACCAGTTGGAACATCTTCTGCGTATATGCCCAGAAGAGG
    TATTGCTGGATCTTCCGGTAGTACTATGTCTAATTTTTTGAGGAACAGCCAGACTGATTTCCAGAGTGGT
    TATACAAGCTTGCAATCCCAACAGCAATGGAGGAGTGTTCCTCTTTCTCCACATCCTTACCAGCATCTGC
    TGTCACCTGAATTTTTGATCTTAGCCATTCTGACTGGTGTGAGGTGGAATCTCAGGGTTGTTTTGATTTG
    CATTTCCCTGATGATTAAGGATGTTGAACATTTTTTCAAGTGCTTCTCAGCCATTCAGTATTCCTCAGTT
    GAGAATTCTTTGTTTAGCTTTGTACCCCATTTTTAATGGGGTTATTTGAATTTCTGGAGTTCAGCTTCTT
    GAGCTCTTTGTATATATTGGATATTAGTCCCCTATCAGATTTAGGATTGGTAAAAATCCTTTCCCAATCT
    GTTGGTGGCCTTTTTGTCTTATTGACAGTGTCTTTTCCCTTACAGAAGCTTTGCAATTTTATGAGGTCCC
    ATTTGTCGATTCTCGATTTTACTGTACAAGCCATTGCTGTTCTGTTCAGGAATTTTTCCCCTGTTCCCAT
    ATCTACGAGGGTTTTTTTCCCACTTTCTCCTCTATAAATTTCAGTGTCTCTGGTTTTATGTGGAGTTCTT
    TGATCCACTTAGACTTGAGCTTTGTACAAGGAGATAAGAATGGATCAATTCGGGCTGGAGAGATGGCTCA
    GTGGTTAAGAGCACTGACTGCTCTTTCAGAAGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGTTCAT
    AACCATCTATAATAAGATCTGATGTCCTTTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATGTA
    ATAAATAAATAAAACTAGATTATTTAAAAAAAAAAAAAAAGAATGGATCAATTCACACTCTTCTACATGA
    TAACCACCAGTTGAGCCCGCACCAATTGTTGAAAATGCTCTCTTTTTCCCACTGGATGGTTTTAGCTCCC
    TTGTCAAAGATCAAGTGACCATAAGTGTGTGGGTTCATTTCTGTGTCTTCAATTGTGTTCCATTGATCTA
    CCTGTCTGTTGCTGTATCAATACCATGCAGTTTTTTTTATCACAATTGCTCTGTAGTACAGCTTGAGGTC
    AGGCATGGTGAGTCCACCAGAGGTTCTTTTATTGTTGAGAATAGTTTTTGCTATCCTAGTTTTTTTTTTT
    TTTTTTTTTTGAACAGCAGGAATCTTATTCTCAAATTGAATCTATTCCTAGAAAGAACTGACTTCCTTTT
    CCATTTTACTTATAGGAAATTTGCCTATTGAAAATTTCCTCTGGTCTTCTGGAGTACCATAGCTACCTGG
    AGTACATGAAGAACAACTTAAAAGATAACAAGAAAGACAAAGCCAGAGTCCTTCAGAGAGATACAGAAAC
    TCTAATTCATATCTTCAACCAAGAGGTGAGTGCTTCCCCATCTCTCATGCAGTGTGGGAAAGAGGACACC
    CGGCACCTCAGGGTAGCGGCACTTTTTCCAGACAGCTGCTCAGAAGGGAGAGAGTCTGAACAACAGTTCT
    TGATTATAAGGCCTTGCTTTGTTTTACTTTGGGGTTTTGTTTGAGGTTCTCTTTTGCAAAGAACATCAAT
    ACCTGCTTTAAACTGTATTAATAGAATGTTACTAATTGCGTAAGAGGTATGAAAACTATGACAGCCATAC
    ATAGTCACCCATTATGAGAGCACAGAGACAAAAGTGACTTTAATATTTAATCCTTGGCAAGTGACATTTT
    TGTAACCAGAGTTCTAATGCAGAGAAGTAGCCAAAAGCTAAAATGTCAGGGAAGGAAGGGATCAAGGGCT
    TCTGGCTACCATTAGCCAGAAGAATGGTAGATATGGAGGATTAATGACTCAGAGTGTGGGCGAACAAAGC
    CAGATGCAATAAGAAGGGCCTGGAATGAAACCCTCTTGCTAAGGCTGCTTTTGCCACTTGTAGTTTCTTG
    CCTTAAACCAGAGAGTTTCCCAATTTAATGTGCACAGGAACCACTTAGGGTCTTGTTCCAGCAGGGTCTT
    ACTTAGGAGGTCTAGGAAGGGAACTAAGATTCATTTTTGTAAACAGCTGCTTGTTTATGACCCTGCTATA
    TAGTATTCAGGCTCCTAAGTAGTGGGGCAAGCCTTCCAGTTAGTCTTCCCCATGCCAGTGGGCCCATGGA
    AGGGTGTTTCCAGACTTCTTCATGCTACCACACTAAGAGACTCTCAACAGAGTCTGAATGGAAACCACGA
    AGGAACACATTTGTTTTAGATTCCTCTGTGCCACCTTTACTGATGGGAGCTTCTGTTTTCCAGTAGAATA
    CAGTATGTTAGCATGGATGCTTGGATAACAGACAAATAAGATGGTGGTGCTGACCTCTGGACGCTTACTC
    TCTAGTGGCAGACAGAACAGTAAGGTTAGAATTCTGTTGCTATTAAAAAACTAATAATAATCACCTTGAA
    AAAGAATGGAGTTGTTAGGCATGGGTCTCTCTCGAGTAAGCTTGGAACAAAAGCATCTCCCCTGGCTTGG
    GTGAGTCAAAGCAGATGGACTTAGCTCGTCTCATTCATTCTAAATTAGAACTTCTTCCCACAGCCCAGAA
    CACGCCACAAGAAAAAAAAAATGTGCAATATTTAACCAGTCTTTGTTTTTTCCTCCTTTAGGTAAAAGAT
    TTACATAAAATAGTCCTTCCTACCCCAATTTCCAATGCTCTCCTAACAGATAAGCTGGAGTCACAGAAGG
    AGTGGCTAAGGACCAAGACCATCCAATTCATCTTGAAATCACTTGAAGAATTTCTAAAAGTCACTTTGAG
    ATCTACTCGGCAAACCTAGTGCGTTATGCCTAAGCATATCAGTTTGTGGACATTCCTCACTGTGGTCAGA
    AAATATATCCTGTTGTCAGGTATCTGACTTATGTTGTTCTCTACGAAGAACTGACAATATGAATGTTGGG
    ACACTATTTTAATTATTTTTAATTTATTGATAATTTAAATAAGTAAACTTTAAGTTAATTTATGATTGAT
    ATTTATTATTTTTATGAAGTGTCACTTGAAATGTTATATGTTATAGTTTTGAAATGATAACCTAAAAATC
    TATTTGATATAAATATTCTGTTACCTAGCCAGATGGTTTCTTGGAATGTATAAGTTTACCTCAATGAATT
    GCTAATTTAAATATGTTTTTAAAGAAATCTTTGTGATGTATTTTTATAATGTTTAGACTGTCTTCAAACA
    AATAAATTATATTATATT
  • Protein Sequence : Show Sequence
    >gi|13624311|ref|NP_112445.1| interleukin-6 precursor [Mus musculus]
    MKFLSARDFHPVAFLGLMLVTTTAFPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELC
    NGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKAR
    VLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQ
    T
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Typhimurium hfq mutant vaccine , Salmonella Typhimurium IppA/IppB/msbB , Salmonella Typhimurium IppB/msbB mutant vaccine
IV. Vaccine Information
1. Aro-deleted S. Typhi
a. Type:
Live, attenuated vaccine
b. Antigen
The antigen used was aro-deleted S. Typhi, a strain designated as CVD 908 (Tacket and Levine, 2007).
c. Gene Engineering of AroD
  • Type: Recombinant protein preparation
  • Description: Inactivation of AroD results in attenuation, and the deletions of both AroC and AroD provide a high level of safety against restoration of pathogenicity by recombination. An aroC/aroD-deleted derivative of S. Typhi strain Ty2 (the parent strain of Ty21a) is designated the strain "CVD 908" (Tacket and Levine, 2007).
  • Detailed Gene Information: Click here.
d. Gene Engineering of AroC
  • Type: Recombinant protein preparation
  • Description: Inactivation of AroC results in attenuation, and the deletions of both AroC and AroD provide a high level of safety against restoration of pathogenicity by recombination. An aroC/aroD-deleted derivative of S. Typhi strain Ty2 (the parent strain of Ty21a) is designated the strain "CVD 908" (Tacket and Levine, 2007).
  • Detailed Gene Information: Click here.
e. Preparation
CVD 908 was created by deleting both aroC and aroD, preventing restoration of pathogenicity by recombination (Tacket and Levine, 2007).
f. Virulence
After a single dose of the vaccine (5 × 104 or 5 × 105 cfu), vaccine bacteremia was not detected in serial blood cultures. Half of the subjects tested with this dose developed serum IgG anti–lipopolysaccharide antibodies and IgA anti-LPS antibody-secreting cells (Tacket and Levine, 2007).
g. Description
The aromatic metabolites, such as aroC and aroD, force Salmonella to be nutritionally dependent on p-aminobenzoic acid and 2,3-dihydroxy, both of which are not available in mammilian tissues, preventing proliferation in the host cells (Tacket and Levine, 2007).
h. Human Response
  • Vaccination Protocol: Each vaccine was ingested and contained 2.2 3 108 organisms of lyophilized vaccine. A second lower dose contained 5 3 107 viable organisms. Volunteers fasted for 90 minutes before and after vaccination. A placebo of sodium bicarbonate was also included (Tacket et al., 2000).
  • Immune Response: After a single oral dose, CVD 908-htrA stimulates vigor-
    ous mucosal, humoral, and cellular immune responses at levels (Tacket et al., 2000).
  • Side Effects: Two volunteers had diarrhea in the first 24 h after ingesting vaccine. Three volunteers had
    diarrhea that occurred later (Tacket et al., 2000).
  • Efficacy: Recipients of the high dose of CVD 908-htrA had a mean of 189 anti-LPS IgA ASC per 106 PBMC. Vigorous IgM and IgG ASC responses were detected among vaccinees. ASC-producing an-ti-H antigen IgA occurred in a majority of participants (Tacket et al., 2000).
  • Host Gene Response of IgA
    • Gene Response: ASCs producing IgA anti-LPS were detected in 100% of recipients of the high-dose vaccine and in 92% of recipients of the lower-dose vaccine. ASCs producing IgA anti-H antigen occurred in 79% and 73% of high- and lower-dose vaccine recipients, respectively (Tacket and Levine, 2007).
    • Detailed Gene Information: Click here.
2. Bovine Rhinotracheitis Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 4109.20)
a. Manufacturer:
Texas Vet Lab, Inc.
b. Type:
Inactivated or "killed" vaccine
c. Status:
Licensed
d. Location Licensed:
USA
e. Host Species for Licensed Use:
Cattle
3. Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 43T5.20)
a. Manufacturer:
Texas Vet Lab, Inc.
b. Type:
Inactivated or "killed" vaccine
c. Status:
Licensed
d. Location Licensed:
USA
e. Host Species for Licensed Use:
Cattle
4. Broilact Chick Salmonella vaccine
a. Tradename:
Broilact
b. Type:
Live, attenuated vaccine
c. Antigen
Broilact is a preparation of the complex mixture of bacteria that inhabit the gut of the healthy adult fowl. The Broilact bacteria are able to live normally in the gut and minimise its colonisation by undesirable and harmful bacteria (Game Bird Health - Broilact).
d. Preparation
Broilact consists of obligately and facultatively anaerobic chicken intestinal bacteria. (Schneitz and Renney, 2003)
e. Description
As a competitive exclusion product, Broilact substantially reduces colonization of the intestine of a host animal by Salmonella infantis (Schneitz and Renney, 2003).
f. Copper Pheasant Response
  • Host Strain: Copper Pheasant
  • Vaccination Protocol: Groups of 10 chicks were given 1.0 mg in a dose volume of 0.3 ml of Broilact through gavage (Schneitz and Renney, 2003).
  • Challenge Protocol: The challenge was created with a 24-hr brain-heart infusion broth culture of a nalidixic-acid-resistant derivative of Salmonella infantis. All birds were challenged 24 hours after initial dosage. Birds in trial 1 received 4.4 x 103 cfu each, while the birds in trials 2 and 3 received 4.3 x 103 cfu each (Schneitz and Renney, 2003).
  • Efficacy: Significant protection was induced by Broilact in all three trials (Schneitz and Renney, 2003).
g. Chicken Response
  • Host Strain: Isa Brown
  • Vaccination Protocol: Two trials of 20 chicks were inoculated through gavage at 20 times the normal dose of Broilact, 0.25 ml (Salvat et al., 1992).
  • Challenge Protocol: The challenge was given to the chicks through gavage using 0.1 ml of an 18-h diluted cuture. The first trial of chicks recieved 7.0 x 104 cfu per chick, while the second trial of chicks was inoculated with 5.7 x 103 cfu per chick (Salvat et al., 1992).
  • Efficacy: The protection factor (PF) was above 6 in the first trial and about 2.6 in the second trial at day 3. Protection at day 7 was extremely high (PF over 36 for trial 1 and P over 45 for trial 2) (Salvat et al., 1992).
5. CVD 908-htrA
a. Type:
Live, attenuated vaccine
b. Antigen
The antigen was htrA-deleted S.Typhi (Tacket and Levine, 2007).
c. Gene Engineering of AroC
  • Type: Recombinant protein preparation
  • Description: HtrA was deleted in Salmonella organisms, which created a mutant that was less virulent and more susceptible to oxidative killing. HtrA encodes a heat-shock protein in Salmonella (Tacket and Levine, 2007).

  • Detailed Gene Information: Click here.
d. Preparation
Formation of CVD 908-htrA was conducted from original CVC 908, and the vaccine created consisted of live lyophilized organisms in single-dose glass vials to be resuspended in buffer solution (Tacket and Levine, 2007).
e. Virulence
CVD 908-htrA retained the immunogenicity of the parent CVD 908, but no vaccine bacteremias were detected among people who received a single dose of 5 × 107, 5 × 108, or 5 × 109 cfu, despite surveillance for bacteremias after vaccination. The immune responses measured by seroconversion rates with IgG anti-LPS after vaccination with CVD 908 and CVD 908-htrA were very similar. Most volunteers developed ASC responses, regardless of the vaccine strain received. Some lymphoproliferative responses to flagella and particulate antigen after CVD 908-htrA vaccination were also observed (Tacket and Levine, 2007).
f. Human Response
  • Vaccination Protocol: In this study, 80 healthy adult outpatient volunteers were randomized to receive, with buffer, a single oral administration of high-dose CVD 908-htrA (4.5 × 108 cfu), lower-dose CVD 908-htrA (5 × 107 cfu), or placebo. On day 28, there was a crossover in which the volunteers who had ingested CVD 908-htrA vaccine on day 0 received placebo and those who had received placebo on day 0 received CVD 908-htrA vaccine in either the high or the lower dose (Tacket and Levine, 2007).
  • Side Effects: The incidence of adverse effects was the same in recipients of placebo, high-dose vaccine, or lower-dose vaccine after vaccination. Diarrhea, a potential concern observed in a small number of vaccine recipients in the uncontrolled, phase 1 study, occurred in 3 (4%) of 76 placebo recipients, 1 (3%) of 39 lower-dose vaccine recipients, and 4 (10%) of 39 high-dose vaccine recipients (a statistically insignificant difference). However, in a secondary analysis of the incidence of symptoms in the first 7 days after vaccination, recipients of the high-dose vaccine were found to be slightly more likely to have had diarrhea (8%) than were recipients of placebo (0%) (P = .04). No recipient of the lower-dose vaccine had diarrhea in the first 7 days after vaccination (Tacket and Levine, 2007).
  • Host Gene Response of IgA
    • Gene Response: ASCs producing IgA anti-LPS were detected in 100% of recipients of the high-dose vaccine and in 92% of recipients of the lower-dose vaccine. ASCs producing IgA anti-H antigen occurred in 79% and 73% of high- and lower-dose vaccine recipients, respectively (Tacket and Levine, 2007).
    • Detailed Gene Information: Click here.
6. CVD 909
a. Type:
Live, attenuated vaccine
b. Status:
Licensed
c. Gene Engineering of HtrA
  • Type: Recombinant protein preparation
  • Description: CVD 909 was constructed by replacing the native promoter of tviA with the strong constitutive promoter Ptac (Wang et al., 2000).
  • Detailed Gene Information: Click here.
d. Preparation
S. Typhi CVD 909 was constructed to express the Vi-antigen. This involved a deletion in the promoter region of tviA and an insertion of the sacB-neo cassette, introduced into the chromosome of CVD 908-htrA by homologous recombination. The replacement of the sacB-neo cassette allowed the replacement of the promoter region with the Ptac promoter in a second homologous recombination event (Wang et al., 2000).
e. Human Response
  • Vaccination Protocol: 24 volunteers received a single oral dose of CVD 909 of 106–109 cfu, and 8 adults received 2 doses of 6 × 109 cfu per dose with buffer 14 days apart (Tacket and Levine, 2007).
  • Immune Response: All volunteers tested developed IgA anti-LPS ASCs. However, only 1 of the volunteers who ingested a single dose and only 1 of the 8 volunteers who ingested 2 doses developed serum IgG anti-Vi .However, IgA anti-Vi ASC responses were detected in a majority of volunteers who received 108–109 cfu of CVD 909. These Vi-specific ASC responses provided evidence that the Vi antigen was expressed and immunologically processed, even if serum antibody responses were realitvely small (Tacket and Levine, 2007).
  • Side Effects: Not noted.
f. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Three groups of 6-week-old BALB/c mice were immunized once intranasally with 1010 CFU of either serovar Typhi strain CVD 908-htrA or CVD 909 or PBS (control) (Wang et al., 2000).
  • Immune Response: The mean titer of IgG anti-Vi was much higher in the mice that received CVD 909. However, GMTs of O antibody after immunization were quite similar in the two groups (CVD 909 and CDV 908-htrA) (Wang et al., 2000).
  • Side Effects: Not noted.
  • Challenge Protocol: Mice were challenged with wild-type serovar Typhi strain Ty2 thirty days after primary immunization (Wang et al., 2000).
  • Efficacy: A single mucosal dose of CVD 909 conferred significantly greater protection than CVD 908-htrA (Wang et al., 2000).
7. CVD 915
a. Type:
Live, attenuated vaccine
b. Antigen
The antigens used in the production of this vaccine are: Recombinant frag C, S. Typhi flagella, S.Typhi LPS, S.Typhi lysate, whole-cell heat phenolyzed S. Typhi, PHA, and BSA (Pasetti et al., 1999).
c. Gene Engineering of Frag C
d. Preparation
CVD 915 was manipulated with electroporation with plasmids pTETner15 and pcDNAtetc. guaBA was deleted from S. Typhi strain 915, which interrupts the biosynthesis of guanine nucleatides (Pasetti et al., 1999).
e. Virulence
High titers of IgG1, IgG2a, and IgG2b were observed against Frag C, elicited by the CVD 915. High titers of serum IgG antibody against S. Typhi flagella and LPS were observed in mice vaccinated with the CVS 915. Titers increased significantly following the booster dose.
f. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: BALB/c mice were injected immunized intranasally with 2x109 CFU of CVD 915 in a 30 microliter volume. Mice were boosted in the same manner 35 days later (Pasetti et al., 1999).
  • Immune Response: Immunization with CVD 915 elicited sensitized lymphoid cells that proliferated in the presence of Frag C. A strong immune response was observed (Pasetti et al., 1999).
  • Challenge Protocol: BALM/c mice were challenged with 50-100 lethal doses of tetanus toxin (Pasetti et al., 1999) .
8. Megan Egg
a. Tradename:
Megan Egg
b. Manufacturer:
Lohman Animal Health International
c. Vaccine Ontology ID:
VO_0000352
d. Type:
Live, attenuated vaccine
e. Status:
Licensed
f. Preparation
Megan Egg is a Salmonella vaccine that that consists of a double gene-deleted S. enterica serovar Typhimurium strain and is formulated fro immunization of pullets. Megan Egg contains the same Megan Vac1 organism but Megan Vac1 is used for younger birds (broilers) (Burns et al).
g. Immunization Route
Coarse spray application in the hatchery, or to birds on the barn floor or in cages, or in the drinking water
9. Megan Vac1
a. Tradename:
Megan Vac1
b. Manufacturer:
Lohman Animal Health International
c. Vaccine Ontology ID:
VO_0001022
d. Type:
Live, attenuated vaccine
e. Status:
Licensed
f. Host Species for Licensed Use:
Chicken
g. Immunization Route
Coarse spray application in the hatchery, or to birds on the barn floor or in cages, or in the drinking water
h. Description
Megan Vac1 is a Salmoneela vaccine that consists of a double gene-deleted S. enterica serovar Typhimurium strain and is designed for protection of broilers to reduce Salmonella contamination (Babu et al., 2004; Burns et al).
10. Newcastle Disease-Bronchitis Mass & Ark Types, Killed Virus Vaccine-Salmonella Enteritidis Bacterin (USDA: 48D7.10)
a. Manufacturer:
Lohmann Animal Health International
b. Type:
Inactivated or "killed" vaccine
c. Status:
Licensed
d. Location Licensed:
USA
e. Host Species for Licensed Use:
Chicken
11. Newcastle Disease-Bronchitis Mass & Ark Types, Killed Virus Vaccine-Salmonella Enteritidis Bacterin (USDA: 48D7.11)
a. Manufacturer:
Lohmann Animal Health International
b. Type:
Inactivated or "killed" vaccine
c. Status:
Licensed
d. Location Licensed:
USA
e. Host Species for Licensed Use:
Chicken
12. Newcastle-Bronchitis Mass Type, Killed Virus Vaccine-Salmonella Enteritidis Bacterin (USDA: 48D6.10)
a. Manufacturer:
Wyeth, Intervet Inc., Biomune Company
b. Type:
Inactivated or "killed" vaccine
c. Status:
Licensed
d. Location Licensed:
USA
e. Host Species for Licensed Use:
Chicken
13. S. typhi DnaJ Protein Vaccine
a. Type:
Subunit vaccine
b. Status:
Research
c. Gene Engineering of DnaJ
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
d. Adjuvant: Freund's emulsified oil adjuvant
e. Immunization Route
Intraperitoneal injection (i.p.)
f. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were immunised on day 0 by injecting 40 μg DnaJ/mouse emulsified in Freund's complete adjuvant (100 μl) i.p. Subsequent booster injections were given by injecting 30 μg DnaJ/mouse emulsified in Freund's incomplete adjuvant i.p. on 7th and 28th days. The control mice received adjuvant alone (Sagi et al., 2006).
  • Challenge Protocol: After 15 days of last immunisation, i.e. 43rd day the mice were challenged with 10 MLD (1 × 105 cells) of S. Typhimurium i.p. The animals were observed daily for 30 days for morbidity and mortality (Sagi et al., 2006).
  • Efficacy: Immunisation of mice with DnaJ derived from Salmonella enterica subsp. enterica serovar Typhi str. CT18 was found to provide 70% protection against lethal challenge by S. typhimurium indicating the possible use of DnaJ as vaccine candidate against typhoid (Sagi et al., 2006).
14. S. typhi GroEL Protein Vaccine
a. Type:
Subunit vaccine
b. Status:
Research
c. Gene Engineering of GroEL
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
d. Adjuvant: complete Freund's adjuvant
e. Immunization Route
Intramuscular injection (i.m.)
f. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice each were immunized i.m. with 10 μg of GroEL/mouse either alone or in combination with alum or CFA on 0, 7th, and 28th day as described earlier. As negative control, group of 10 mice was injected with endotoxin free water mixed with alum or CFA (Bansal et al., 2010).
  • Challenge Protocol: Two weeks after the last booster, all the groups were challenged with a lethal dose of 1 × 108 CFU/ml/mouse S. typhi Ty2 intraperitoneally (i.p.). The mice were observed daily for 30 days for any morbidity or mortality (Bansal et al., 2010).
  • Efficacy: Immunization of mice with GroEL + CFA provided 80% protection against lethal challenge by S. typhi in mice (Bansal et al., 2010).
15. S. typimurium Vi4072
a. Type:
Recombinant vector vaccine
b. Status:
Research
c. Gene Engineering of TviB
  • Type: Recombinant vector construction
  • Description:
  • Detailed Gene Information: Click here.
d. Vector:
Salmonella typhimurium χ4072, an attenuated Δcya Δcrp mutant (Cao et al., 1992).
e. Immunization Route
Orally
f. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were immunized orally with 5 x 107 recombinant Salmonella Vi4072. As controls, groups of mice were inoculated orally with 5 x 107 χ4072(pYA248) or BSG (Cao et al., 1992).
  • Challenge Protocol: At 60 days postimmunization, mice were challenged with virulent S. typhi Ty2 (Cao et al., 1992).
  • Efficacy: Immunization of mice with Vi4072 afforded complete protection against fatal infection with virulent S. typhi Ty2 (Cao et al., 1992).
  • Description: The viaB (tviB) gene coding for the Vi antigen of Salmonella typhi Ty2 was subcloned into expression vector pYA248. The recombinant plasmid was termed SMM202 and transformed into Salmonella typhimurium Vi4072, an attenuated ΔcyaΔcrp mutant (Cao et al., 1992).
16. Salmonella Choleraesuis Avirulent Live Culture Vaccine (USDA: 19A1.00)
a. Manufacturer:
Boehringer Ingelheim Vetmedica, Inc., Intervet Inc., Novartis Animal Health US, Inc., Arko Laboratories Ltd.
b. Type:
Live vaccine
c. Status:
Licensed
d. Location Licensed:
USA
e. Host Species for Licensed Use:
Cattle
17. Salmonella Choleraesuis Avirulent Live Culture Vaccine (USDA: 19A1.01)
a. Manufacturer:
Boehringer Ingelheim Vetmedica, Inc., Arko Laboratories Ltd.
b. Type:
Live vaccine
c. Status:
Licensed
d. Location Licensed:
USA
e. Host Species for Licensed Use:
Cattle
18. Salmonella DNA vaccine encoding SopB Protein
a. Type:
DNA vaccine
b. Status:
Research
c. Gene Engineering of SopB
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
d. Vector:
pCI-TPA (Nagarajan et al., 2009)
e. Immunization Route
Intraperitoneal injection (i.p.)
f. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Six- to eight-week-old female BALB/c mice were immunized via i.p. route with 1 ml of PBS containing 103 CFU of ΔpmrG-HM-D strain per mouse and 10 μg of DNA vaccine plasmid in 50 μl volume of PBS in cohort of five mice. Booster doses were given on 7th and 14th day. Placebo control mice were injected with 103 CFU of ΔpmrG-HM-D strain per mouse and 10 μg of empty pCI-TPA vector (Nagarajan et al., 2009).
  • Challenge Protocol: 21 days after immunization, mice were challenged with 107 CFU/mouse of WT Salmonella orally (Nagarajan et al., 2009).
  • Efficacy: SopB DNA vaccine immunization reduced the bacterial burden of organs by about 5-fold on day 4 and day 8 after challenge with virulent Salmonella and proved to be a more efficient vaccination strategy than live attenuated bacteria alone (Nagarajan et al., 2009).
19. Salmonella Dublin Live Culture Vaccine (USDA: 19D1.R0)
a. Manufacturer:
Wyeth
b. Type:
Live vaccine
c. Status:
Licensed
d. Location Licensed:
USA
e. Host Species for Licensed Use:
Cattle
20. Salmonella enterica fliC/guaB mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse, chicken
d. Gene Engineering of fliC
e. Gene Engineering of guaB from S. enteritidis str. P125109
f. Immunization Route
Intramuscular injection (i.m.)
g. Mouse Response
  • Persistence: A fliC/guaB mutant is attenuated in mice (Adriaensen et al., 2007).
  • Efficacy: A fliC/guaB mutant induces significant protection in mice from challenge with wild type S. enterica (Adriaensen et al., 2007).
  • Host Gene Response of IgG
    • Gene Response: Fifty-four days following initial oral immunization of BALB/c mice with approximately 108 CFU per mouse of, respectively, the S. enterica serovar Enteritidis ΔguaB and S. enterica serovar Enteritidis ΔguaB ΔfliC mutants, blood samples were collected from the tail arteries of five mice. Comparison between sera of mice immunized with S. enterica serovar Enteritidis ΔguaB and mice immunized with S. enterica serovar Enteritidis ΔguaB ΔfliC showed that, in both cases, comparable anti-LPS serum IgG responses were elicited (Adriaensen et al., 2007).
    • Detailed Gene Information: Click here.
h. Chicken Response
21. Salmonella Enteritidis guaBA/clpP mutant vaccine
a. Product Name:
CVD 1941
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of clpP
  • Type: Gene mutation
  • Description: This guaBA/clpP mutant is from Salmonella Enteritidis (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
f. Gene Engineering of guaA
  • Type: Gene mutation
  • Description: This guaBA/clpP mutant is from Salmonella Enteritidis (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
g. Gene Engineering of guaB from S. enteritidis str. P125109
  • Type: Gene mutation
  • Description: This guaBA/clpP mutant is from Salmonella Enteritidis (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
h. Immunization Route
Oral immunization
i. Mouse Response
  • Persistence: A guaBA/clpP mutant is attenuated in mice (Tennant et al., 2011).
  • Efficacy: A guaBA/clpP mutant induces protection in mice from challenge with wild type Salmonella Enteritidis. The vaccine efficacy is 76% (Tennant et al., 2011).
  • Host Gene Response of IgG
    • Gene Response: Immune responses induced included high levels of serum IgG anti-lipopolysaccharide (LPS), with titers increasing progressively during the immunization schedule. For mice immunized with attenuated S. Enteritidis vaccine candidates, there was a trend of higher seroconversion rates after the first dose (26 days after immunization) in mice that received CVD 1941 than for those immunized with CVD 1943, which was statistically significant for anti-FliC (Tennant et al., 2011).
    • Detailed Gene Information: Click here.
22. Salmonella Gallinarum cbiA/cobS mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Chickens
d. Gene Engineering of cbiA
  • Type: Gene mutation
  • Description: This cbiA/cobS mutant is from Salmonella Gallinarum (Penha et al., 2010).
  • Detailed Gene Information: Click here.
e. Gene Engineering of cobS
  • Type: Gene mutation
  • Description: This cbiA/cobS mutant is from Salmonella Gallinarum (Penha et al., 2010).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intramuscular injection (i.m.)
g. Chicken Response
  • Persistence: A cbiA/cobS mutant is attenuated in chickens (Penha et al., 2010).
  • Efficacy: A cbiA/cobS mutant induces significant protection in chickens from challenge with wild type S. Gallinarum (Penha et al., 2010).
23. Salmonella Gallinarum cpxR/Ion mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of cpxR
e. Gene Engineering of lon
f. Immunization Route
Intramuscular injection (i.m.)
g. Mouse Response
  • Persistence: A cpxR/Ion mutant is highly attenuated in mice (Matsuda et al., 2010).
  • Efficacy: A cpxR/Ion mutant induces significant protection in mice from challenge with wild type S. Gallinarum (Matsuda et al., 2010).
24. Salmonella Gallinarum crp mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of crp
  • Type: Gene mutation
  • Description: This crp mutant is from Salmonella Gallinarum (Rosu et al., 2007).
  • Detailed Gene Information: Click here.
e. Immunization Route
Intramuscular injection (i.m.)
f. Mouse Response
  • Persistence: A crp mutant is attenuated in mice (Rosu et al., 2007).
  • Efficacy: A crp mutant induces significant protection in mice from challenge with wild type S. Gallinarum (Rosu et al., 2007).
25. Salmonella Gallinarum nuoG mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Chickens
d. Gene Engineering of nuoG
e. Immunization Route
Intramuscular injection (i.m.)
f. Chicken Response
26. Salmonella Gallinarum relA/spoT mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Chickens
d. Gene Engineering of relA
  • Type: Gene mutation
  • Description: This relA/spoT mutant is from Salmonella Gallinarum (Park et al., 2010).
  • Detailed Gene Information: Click here.
e. Gene Engineering of spoT
  • Type: Gene mutation
  • Description: This relA/spoT mutant is from Salmonella Gallinarum (Park et al., 2010).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intramuscular injection (i.m.)
g. Chicken Response
  • Persistence: A relA/spoT mutant is attenuated in chickens (Park et al., 2010).
  • Efficacy: A relA/spoT mutant induces significant protection in chickens from challenge with wild type Salmonella Gallinarum (Park et al., 2010).
  • Host Gene Response of IgA Fc receptor
    • Gene Response: Serum IgG and IgA peaked at 3-4 wpi and was a significant antibody response against Salmonella vs. unimmunized chickens (Park et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG Fc fragment
    • Gene Response: Serum IgG and IgA peaked at 3-4 wpi and was a significant antibody response against Salmonella vs. unimmunized chickens (Park et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgM Fc receptor
    • Gene Response: The serum IgM levels against Salmonella in all chickens immunized with the mutant strains increased up to 3 weeks post infection (wpi), and subsequently decreased. The titers were significant vs. unimmunized chickens (Park et al., 2010).
    • Detailed Gene Information: Click here.
27. Salmonella IroN Protein Vaccine
a. Type:
Subunit vaccine
b. Status:
Research
c. Gene Engineering of IroN
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
d. Adjuvant: incomplete Freund's adjuvant
e. Immunization Route
Intramuscular injection (i.m.)
f. Chicken Response
  • Host Strain: white leghorn
  • Vaccination Protocol: Ten 30-day-old specific-pathogen-free (SPF) white leghorn chickens were immunized intramuscularly with 0.5 ml of purified IroN (0.05 mg/ml), homogenized with Freund's incomplete adjuvant, and 10 control chickens were immunized intramuscularly with PBS homogenized with Freund's incomplete adjuvant. The immunized chickens were given an intramuscular booster injection 3 wk after the first immunization (Kaneshige et al., 2009).
  • Challenge Protocol: Three weeks after the second injection, the immunized chickens and controls were challenged intravenously with 108 CFU of Salmonella Enteritidis (Kaneshige et al., 2009).
  • Efficacy: The mortality rate of immunized chickens was 10%, while that of control chickens was 80% (Kaneshige et al., 2009).
28. Salmonella typhi aroA mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of aroA
  • Type: Gene mutation
  • Description: This aroA was mutated from Salmonella typhi strain Ty2 (Dougan et al., 1987).
  • Detailed Gene Information: Click here.
e. Immunization Route
Intraperitoneal injection (i.p.)
f. Mouse Response
  • Persistence: An aroA mutant is attenuated in mice (Dougan et al., 1987).
  • Efficacy: An aroA mutant induced significant protection in mice from challenge with wild type S. typhi Ty2 (Dougan et al., 1987).
29. Salmonella Typhi galE mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of galE
e. Immunization Route
Intraperitoneal injection (i.p.)
f. Mouse Response
30. Salmonella Typhi phoP mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of PhoP
  • Type: Gene mutation
  • Description: This phoP mutant is from Salmonella Typhi (Lee et al., 2007).
  • Detailed Gene Information: Click here.
e. Immunization Route
Intraperitoneal injection (i.p.)
f. Mouse Response
  • Persistence: A phoP mutant is attenuated in mice (Lee et al., 2007).
  • Efficacy: A phoP mutant provides significant protection in mice from challenge with wild type S. Typhi (Lee et al., 2007).
  • Host Gene Response of IgG
    • Gene Response: The serum titer of IgG was more than 1000-fold higher in mice immunized with a phoP mutant than in control mice. The mutant induced high titers of serum IgG against lipopolysaccharide of S. typhi at 8 weeks in the immunization schedule (Lee et al., 2007).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG2b
    • Gene Response: The titers of serum IgG2b increased significantly 8 weeks after inoculation in mice immunized with a phoP mutant as compared to unimmunized control mice (Lee et al., 2007).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ighv1-9
    • Gene Response: Titers of serum IgG2a increased significantly 8 weeks after inoculation in mice immunized with a phoP mutant as compared to unimmunized control mice (Lee et al., 2007).
    • Detailed Gene Information: Click here.
31. Salmonella Typhimurium asd mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of asd
  • Type: Gene mutation
  • Description: This asd mutant is from Salmonella Typhimurium (Piao et al., 2010).
  • Detailed Gene Information: Click here.
e. Immunization Route
Oral immunization
f. Mouse Response
  • Persistence: An asd mutant is attenuated in mice (Piao et al., 2010).
  • Efficacy: An asd mutant induces significant protection in mice from challenge with wild type Salmonella typhimurium (Piao et al., 2010).
  • Host Gene Response of IgA
    • Gene Response: Immunizing twice elicited significantly higher levels of IgA than immunizing once, and oral immunization was the most effective route in comparison to i.p. immunization. IgA levels for both oral and i.p. routes were significantly higher than unimmunized control mice titers. Sera was obtained two weeks after immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: Immunizing twice instead of once induced significantly higher levels of IgG via the oral route compared to non-immunized mice. Sera was obtained 2 weeks after immunization. I.p. immunization induced higher levels of IgG antibodies than oral immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ighg1
    • Gene Response: Immunizing twice instead of once induced significantly higher levels of IgG1 via the oral route compared to non-immunized mice. Sera was obtained 2 weeks after immunization. I.p. immunization induced higher levels of IgG1 antibodies than oral immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ighv1-9
    • Gene Response: Immunizing twice instead of once induced significantly higher levels of IgG2a via the oral route compared to non-immunized mice. Sera was obtained 2 weeks after immunization. I.p. immunization induced higher levels of IgG2a antibodies than oral immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-17
    • Gene Response: Splenocytes from mice immunized via the oral route showed significantly high levels of IL-17 compared to non-immunized mice. Splenocytes were isolated and stimulated in vitro for 24 hours with wild type S. Typhimurium lysate. Immunizing twice was more effective than immunizing once (Piao et al., 2010).
    • Detailed Gene Information: Click here.
32. Salmonella Typhimurium asd/rfc mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of asd
  • Type: Gene mutation
  • Description: This asd/rfc mutant is from Salmonella Typhimurium (Piao et al., 2010).
  • Detailed Gene Information: Click here.
e. Gene Engineering of rfc
  • Type: Gene mutation
  • Description: This asd/rfc mutant is from Salmonella Typhimurium (Piao et al., 2010).
  • Detailed Gene Information: Click here.
f. Immunization Route
Oral immunization
g. Mouse Response
  • Persistence: An asd/rfc mutant is attenuated in mice (Piao et al., 2010).
  • Efficacy: An asd/rfc mutant induces significant protection in mice from challenge with wild type S. typhimurium (Piao et al., 2010).
  • Host Gene Response of IgA
    • Gene Response: Immunizing twice elicited significantly higher levels of IgA than immunizing once, and oral immunization was the most effective route in comparison to i.p. immunization. IgA levels for both oral and i.p. routes were significantly higher than unimmunized control mice titers. Sera was obtained two weeks after immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: Immunizing twice instead of once induced significantly higher levels of IgG via the oral route compared to non-immunized mice. Sera was obtained 2 weeks after immunization. I.p. immunization induced higher levels of IgG antibodies than oral immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ighg1
    • Gene Response: Immunizing twice instead of once induced significantly higher levels of IgG1 via the oral route compared to non-immunized mice. Sera was obtained 2 weeks after immunization. I.p. immunization induced higher levels of IgG1 antibodies than oral immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ighv1-9
    • Gene Response: Immunizing twice instead of once induced significantly higher levels of IgG2a via the oral route compared to non-immunized mice. Sera was obtained 2 weeks after immunization. I.p. immunization induced higher levels of IgG2a antibodies than oral immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-17
    • Gene Response: Splenocytes from mice immunized via the oral route showed significantly high levels of IL-17 compared to non-immunized mice. Splenocytes were isolated and stimulated in vitro for 24 hours with wild type S. Typhimurium lysate. Immunizing twice was more effective than immunizing once (Piao et al., 2010).
    • Detailed Gene Information: Click here.
33. Salmonella Typhimurium atpA mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of atpA
e. Immunization Route
Intravenous injection (i.v.)
f. Mouse Response
34. Salmonella Typhimurium clpP mutant vaccine
a. Product Name:
CS2007
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of clpP from S. typhimurium str. LT2
f. Immunization Route
Oral immunization
g. Mouse Response
  • Persistence: A clpP mutant (CS2007) causes hyperflagellation. These hyperflagellated bacteria are attenuated in mice (Matsui et al., 2003).
  • Efficacy: A clpP mutant elicited protection in mice from challenge with wild type S. typhimurium (Matsui et al., 2003).
  • Host Gene Response of IgA
    • Gene Response: Significant increases of lipopolysaccharide-specific immunoglobulin G (IgG) and secretory IgA were detected at week 4 and maintained until at least week 12 after inoculation in serum and bile. Testing before week 4 revealed undetectable or extremely low levels of antibodies, meaning the presence of much higher levels after week 4 were the result of upregulation of IgG and IgA (Matsui et al., 2003).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: Significant increases of lipopolysaccharide-specific immunoglobulin G (IgG) and secretory IgA were detected at week 4 and maintained until at least week 12 after inoculation in serum and bile. Testing before week 4 revealed undetectable or extremely low levels of antibodies, meaning the presence of much higher levels after week 4 were the result of upregulation of IgG and IgA (Matsui et al., 2003).
    • Detailed Gene Information: Click here.
35. Salmonella Typhimurium cya/crp mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of crp from S. Typhimurium
e. Gene Engineering of cya
f. Immunization Route
Peroral Inoculation
g. Mouse Response
36. Salmonella Typhimurium guaBA/clpP mutant vaccine
a. Product Name:
CVD 1921
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of clpP from S. typhimurium str. LT2
  • Type: Gene mutation
  • Description: This guaBA/clpP mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
f. Gene Engineering of guaA from S. typhimurium str. LT2
  • Type: Gene mutation
  • Description: This guaBA/clpP mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
g. Gene Engineering of guaB
  • Type: Gene mutation
  • Description: This guaBA/clpP mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
h. Immunization Route
Oral immunization
i. Mouse Response
  • Persistence: A guaBA/clpP mutant is attenuated in mice (Tennant et al., 2011).
  • Efficacy: A guaBA/clpP mutant induces protection in mice from challenge with wild type Salmonella Typhimurium. The vaccine efficacy is 86% (Tennant et al., 2011).
  • Host Gene Response of IgG
    • Gene Response: Immune responses induced included high levels of serum IgG anti-lipopolysaccharide (LPS), with titers increasing progressively during the immunization schedule. Mice immunized with attenuated S. Typhimurium construct CVD 1921 had excellent seroconversion rates after the second immunization and mounted robust antibody responses against both target antigens (LPS and FliC) (Tennant et al., 2011).
    • Detailed Gene Information: Click here.
37. Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine
a. Product Name:
CVD 1923
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of clpP from S. typhimurium str. LT2
  • Type: Gene mutation
  • Description: This guaBA/clpP/fliD mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
f. Gene Engineering of fliD
  • Type: Gene mutation
  • Description: This guaBA/clpP/fliD mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
g. Gene Engineering of guaA from S. typhimurium str. LT2
  • Type: Gene mutation
  • Description: This guaBA/clpP/fliD mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
h. Gene Engineering of guaB
  • Type: Gene mutation
  • Description: This guaBA/clpP/fliD mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
i. Immunization Route
Oral immunization
j. Mouse Response
  • Persistence: A guaBA/clpP/fliD mutant is attenuated in mice (Tennant et al., 2011).
  • Efficacy: A guaBA/clpP/fliD mutant induces protection in mice from challenge with wild type Salmonella Typhimurium. The vaccine efficacy is 80% (Tennant et al., 2011).
  • Host Gene Response of IgG
    • Gene Response: Immune responses induced included high levels of serum IgG anti-lipopolysaccharide (LPS), with titers increasing progressively during the immunization schedule. Mice immunized with attenuated S. Typhimurium construct CVD 1923 had excellent seroconversion rates after the second immunization and mounted robust antibody responses against both target antigens (LPS and FliC) (Tennant et al., 2011).
    • Detailed Gene Information: Click here.
38. Salmonella Typhimurium gyrA/cpxA/rpoB mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Pigs
d. Gene Engineering of cpxA
  • Type: Gene mutation
  • Description: This gyrA/cpxA/rpoB mutant is from Salmonella Typhimurium (Roesler et al., 2004).
  • Detailed Gene Information: Click here.
e. Gene Engineering of gyrA
  • Type: Gene mutation
  • Description: This gyrA/cpxA/rpoB mutant is from Salmonella Typhimurium (Roesler et al., 2004).
  • Detailed Gene Information: Click here.
f. Gene Engineering of rpoB
  • Type: Gene mutation
  • Description: This gyrA/cpxA/rpoB mutant is from Salmonella Typhimurium (Roesler et al., 2004).
  • Detailed Gene Information: Click here.
g. Immunization Route
Oral immunization
h. Pig Response
  • Persistence: A gyrA/cpxA/rpoB mutant was attenuated in pigs (Roesler et al., 2004).
  • Efficacy: A gyrA/cpxA/rpoB mutant protected pigs against challenge with wild type Salmonella Typhimurium (Roesler et al., 2004).
  • Host Gene Response of IgM
    • Gene Response: A significant discrimination between vaccinated and non-vaccinated pigs was observed when IgM was measured after challenge, but here, the non-immunized animals had an elevated humoral antibody response after challenge with wild type Salmonella. The first significant difference was seen on day 35 after vaccination (Roesler et al., 2004).
    • Detailed Gene Information: Click here.
39. Salmonella Typhimurium hfq mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of hfq
  • Type: Gene mutation
  • Description: This hfq mutant is from Salmonella Typhimurium (Allam et al., 2011).
  • Detailed Gene Information: Click here.
e. Immunization Route
Oral immunization
f. Mouse Response
  • Persistence: An hfq mutant is attenuated in mice (Allam et al., 2011).
  • Efficacy: An hfq mutant efficiently protects mice against subsequent oral challenge with virulent strain of Salmonella Typhimurium (Allam et al., 2011).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: Vaccinated mice had significantly higher serum IFN-γ and IL-6 than the PBS administered mice. The serum IL-6 and IFN-γ levels of vaccinated mice further increased upon challenge while remaining lower than that of unvaccinated challenged mice. Serum was collected 7 days after vaccination from unchallenged mice or 7 days after challenge from challenged mice (Allam et al., 2011).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgA
    • Gene Response: Mucosal IgA and serum IgG levels significantly increased in vaccinated mice compared to naive mice. The antibody titers remained high in case of mice vaccinated and challenged compared with the unvaccinated and challenged mice. The elicited humoral immune response remained 4 weeks after immunization with mutant strain (Allam et al., 2011).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: Mucosal IgA and serum IgG levels significantly increased in vaccinated mice compared to naive mice. The antibody titers remained high in case of mice vaccinated and challenged compared with the unvaccinated and challenged mice. The elicited humoral immune response remained 4 weeks after immunization with mutant strain (Allam et al., 2011).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-6
    • Gene Response: Vaccinated mice had significantly higher serum IFN-γ and IL-6 than the PBS administered mice. The serum IL-6 and IFN-γ levels of vaccinated mice further increased upon challenge while remaining lower than that of unvaccinated challenged mice. Serum was collected 7 days after vaccination from unchallenged mice or 7 days after challenge from challenged mice (Allam et al., 2011).
    • Detailed Gene Information: Click here.
40. Salmonella Typhimurium htrA mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of htrA
e. Immunization Route
Intravenous injection (i.v.)
f. Mouse Response
41. Salmonella Typhimurium IppA/IppB/msbB
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of lppA
  • Type: Gene mutation
  • Description: This IppA/IppB/msbB mutant is from Salmonella Typhimurium (Liu et al., 2008).
  • Detailed Gene Information: Click here.
e. Gene Engineering of lppB
  • Type: Gene mutation
  • Description: This IppA/IppB/msbB mutant is from Salmonella Typhimurium (Liu et al., 2008).
  • Detailed Gene Information: Click here.
f. Gene Engineering of msbB
  • Type: Gene mutation
  • Description: This IppA/IppB/msbB mutant is from Salmonella Typhimurium (Liu et al., 2008).
  • Detailed Gene Information: Click here.
g. Immunization Route
Intraperitoneal injection (i.p.)
h. Mouse Response
  • Persistence: An IppA/IppB/msbB mutant is attenuated in mice (Liu et al., 2008).
  • Efficacy: An IppA/IppB/msbB mutant provides significant protection in mice from challenge with wild type S. Typhimurium (Liu et al., 2008).
  • Host Gene Response of Ighg1
    • Gene Response: This mutant elicited a significantly greater serum IgG1 response compared to infection with the wild type on days 2, 5, and 7 post infection. Peak levels were reached five days after infection (Liu et al., 2008).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-6
    • Gene Response: Culture supernatants from T cells infected with the lppB/msbB mutant contained significantly higher levels of IL-6 than did supernatants from T cells that were infected with WT S. Typhimurium (Liu et al., 2008).
    • Detailed Gene Information: Click here.
42. Salmonella Typhimurium IppB/msbB mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of lppB
  • Type: Gene mutation
  • Description: This IppB/msbB mutant is from Salmonella Typhimurium (Liu et al., 2008).
  • Detailed Gene Information: Click here.
e. Gene Engineering of msbB
  • Type: Gene mutation
  • Description: This IppB/msbB mutant is from Salmonella Typhimurium (Liu et al., 2008).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: An IppB/msbB mutant is attenuated in mice (Liu et al., 2008).
  • Efficacy: An IppB/msbB mutant provides significant protection from challenge with wild type S. Typhimurium (Liu et al., 2008).
  • Host Gene Response of Ighg1
    • Gene Response: This mutant elicited a significantly greater serum IgG1 response compared to infection with the wild type on day 14 after vaccination. Peak levels were reached five days after infection (Liu et al., 2008).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-6
    • Gene Response: Culture supernatants from T cells infected with the lppB/msbB mutant contained significantly higher levels of IL-6 than did supernatants from T cells that were infected with WT S. Typhimurium (Liu et al., 2008).
    • Detailed Gene Information: Click here.
43. Salmonella Typhimurium Live Culture Vaccine (USDA: 19C1.00)
a. Manufacturer:
Wyeth
b. Type:
Live vaccine
c. Status:
Licensed
d. Location Licensed:
USA
e. Host Species for Licensed Use:
Chicken
44. Salmonella Typhimurium Live Culture Vaccine (USDA: 19C1.01)
a. Manufacturer:
Lohmann Animal Health International
b. Type:
Live vaccine
c. Status:
Licensed
d. Location Licensed:
USA
e. Host Species for Licensed Use:
Chicken
45. Salmonella Typhimurium Live Culture Vaccine (USDA: 19C1.02)
a. Manufacturer:
Lohmann Animal Health International, Biomune Company
b. Type:
Live vaccine
c. Status:
Licensed
d. Location Licensed:
USA
e. Host Species for Licensed Use:
Chicken
46. Salmonella Typhimurium ompD/nmpC mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Pig
d. Gene Engineering of NmpC
  • Type: Gene mutation
  • Description: This ompD/nmpC mutant is from Salmonella Typhimurium (Selke et al., 2007).
  • Detailed Gene Information: Click here.
e. Immunization Route
Oral immunization
f. Pig Response
  • Persistence: An ompD/nmpc mutant is attenuated in pigs (Selke et al., 2007).
  • Efficacy: In a pig infection experiment including two oral immunizations with SalmoporcDeltaompD (OmpD, nmpC) and challenge with a multiresistant S. enterica serovar Typhimurium DT104 clinical isolate, we confirmed the protective efficacy of SalmoporcDeltaompD (Selke et al., 2007).
47. Salmonella Typhimurium ompR mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of ompR
e. Immunization Route
Intravenous injection (i.v.)
f. Mouse Response
  • Persistence: An ompR mutant is attenuated in mice after oral challenge (Dorman et al., 1989).
  • Efficacy: A ompR mutant conferred significant protection in mice from challenge with wild type Salmonella Typhimurium (Dorman et al., 1989).
48. Salmonella Typhimurium poxA mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of poxA
e. Immunization Route
Intraperitoneal injection (i.p.)
f. Mouse Response
  • Persistence: A poxA mutant is attenuated in mice (Kaniga et al., 1998).
  • Efficacy: A poxA mutant provided significant protection in mice from challenge with wild type Salmonella Typhimurium (Kaniga et al., 1998).
  • Host Gene Response of IgA
    • Gene Response: Sera were collected 29 days after a single oral immunization and 16 days post challenge. High IgG, IgA, and, to a lesser extent, IgM titers were detected in orally immunized animals, indicating that the ΔpoxA270 mutant derivative of S. typhimurium was very immunogenic in mice. This was more than negative control serum collected from non immunized mice (Kaniga et al., 1998).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: Sera were collected 29 days after a single oral immunization and 16 days post challenge. High IgG, IgA, and, to a lesser extent, IgM titers were detected in orally immunized animals, indicating that the ΔpoxA270 mutant derivative of S. typhimurium was very immunogenic in mice. This was significantly more than negative control serum collected from non immunized mice (Kaniga et al., 1998).
    • Detailed Gene Information: Click here.
49. Salmonella Typhimurium rpoS mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of rpoS
e. Immunization Route
Intraperitoneal injection (i.p.)
f. Mouse Response
  • Persistence: An rpoS mutant is attenuated in mice (Coynault et al., 1996).
  • Efficacy: An rpoS mutant protects mice from challenge with wild type Salmonella typhimurium after a single dose (Coynault et al., 1996).
50. Salmonella Typhimurium ruvB mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of ruvB
  • Type: Gene mutation
  • Description: This ruvB mutant is from Salmonella Typhimurium (Choi et al., 2010).
  • Detailed Gene Information: Click here.
e. Immunization Route
Intraperitoneal injection (i.p.)
f. Mouse Response
  • Persistence: A ruvB mutant is attenuated in mice (Choi et al., 2010).
  • Efficacy: A ruvB mutant provides protection in mice from challenge with wild type Salmonella Typhimurium (Choi et al., 2010).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: The vaccine induced significant production of IFN-γ on days 18 and 22 after vaccination while the control mice (non vaccinated) showed no change in the level of IFN-γ (Choi et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: Total IgG levels were significantly increased in vaccinated mice compared to the non-vaccinated group at both day 18 and day 22 (Choi et al., 2010).
    • Detailed Gene Information: Click here.
51. Salmonella Typhimurium surA mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of surA
e. Immunization Route
Intravenous injection (i.v.)
f. Mouse Response
52. Salmonella Typhimurium tolA mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of tolA
e. Immunization Route
Intravenous injection (i.v.)
f. Mouse Response
53. Salmonella Typhimurium trxA mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of trxA
  • Type: Gene mutation
  • Description: A trxA mutant is attenuated in mice and induced significant protection from challenge with wild type S. Typhimurium (Peters et al., 2010).
  • Detailed Gene Information: Click here.
e. Immunization Route
Intravenous injection (i.v.)
f. Mouse Response
  • Persistence: A trxA mutant is attenuated in mice (Peters et al., 2010).
  • Efficacy: A trxA mutant induces protection in mice from challenge with wild type Salmonella Typhimurium (Peters et al., 2010).
54. Salmonella Typhimurium wecA mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of wecA
e. Immunization Route
Intraperitoneal injection (i.p.)
f. Mouse Response
  • Persistence: A wecA mutant (DSM645) is attenuated in C57BL/6J mice through i.p. and oral immunization (Gilbreath et al., 2011).
  • Efficacy: A wecA mutant (DSM645) protects against challenge with S. typhimurium strains TML and SL1344 30 days after immunization (Gilbreath et al., 2011).
55. Salmonella Typhimurium znuABC mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of znuA
e. Gene Engineering of znuB
f. Gene Engineering of znuC
g. Immunization Route
Oral immunization
h. Mouse Response
56. Salmonella vacT
a. Tradename:
vacT
b. Type:
Live, attenuated vaccine
c. Antigen
Salmonella vacT is made of S. Typhimurium (Methner et al., 1997).
d. Chicken Response
  • Vaccination Protocol: A group of one-day-old SPF chicks were innoculated orally with 0.1 ml of a vacT culture such that each bird received between 6 x 108 and 1 x 109 cfu each (Methner et al., 1997).
  • Challenge Protocol: The birds were challenged 24 h later orally with 0.1 ml of nalidixic acid-resistant Salmonella derivative diluted in PBS such that each bird recieved 104 cfu (Methner et al., 1997).
  • Efficacy: A significant amount of S. Typhimurium 421 was found in the livers and the caeca of the birds days after the challenge (Methner et al., 1997).
57. Typherix
a. Tradename:
Typherix
b. Manufacturer:
GlaxoSmithKline
c. Vaccine Ontology ID:
VO_0010744
d. Type:
Subunit vaccine
e. Status:
Licensed
f. Location Licensed:
Canada
g. Host Species for Licensed Use:
Human
h. Preservative:
Phenol
i. Allergen:
Latex in plunger stopper of pre-filled syringes
j. Immunization Route
Intramuscular injection (i.m.)
k. Description
Products: Polysaccharide.
58. Typhim Vi
a. Product Name:
Typhoid Vi Polysaccharide Vaccine
b. Tradename:
Typhim Vi
c. Manufacturer:
Sanofi Pasteur SA
d. Vaccine Ontology ID:
VO_0000104
e. CDC CVX code:
101
f. Type:
Subunit vaccine
g. Status:
Licensed
h. Location Licensed:
USA (License #1724)
i. Host Species for Licensed Use:
Human
j. Antigen
The Vi-antigen is a polysaccharide capsule found only in S. enterica serovars Dublin, Typhi, and Paratyphi C The Vi-antigen is a polysaccharide capsule found only in S. enterica serovars Dublin, Typhi, and Paratyphi C (Cheminay and Hensel, 2007).
k. Preservative:
Phenol
l. Immunization Route
Intramuscular injection (i.m.)
m. Storage
Store at 2° to 8°C (35° to 46°F). DO NOT FREEZE.
n . Approved Age for Licensed Use
Two years of age and older.
o. Contraindication
TYPHIM Vi VACCINE IS CONTRAINDICATED IN PATIENTS WITH A HISTORY OF HYPERSENSITIVITY TO ANY COMPONENT OF THIS VACCINE (Typhim Vi).
p. Description
Contains the cell surface Vi polysaccharide extracted from Salmonella enterica serovar Typhi, S typhi Ty2 strain. The organism is grown in a semi-synthetic medium without animal proteins. The capsular polysaccharide is precipitated from the concentrated culture supernatant by the addition of hexadecyltrimethylammonium bromide and the product is purified by differential centrifugation and precipitation (Typhim Vi).
q. Human Response
  • Side Effects: The most common side effects reported are: injection site reactions, fever, headache, nausea, and malaise (Typhim Vi).
  • Efficacy: The overall protective efficacy of Typhim Vi vaccine was 74% confirmed by a blood culture of confirmed cases of typhoid fever during 20 months of post-vaccination follow-up (Typhim Vi).
59. ViVaxim
a. Product Name:
Combined Purified Vi Polysaccharide Typhoid and Inactivated Hepatitis A Vaccine
b. Tradename:
ViVaxim
c. Manufacturer:
Sanofi Pasteur Ltd
d. Vaccine Ontology ID:
VO_0010748
e. Type:
Subunit vaccine + Inactivated or "killed" vaccine
f. Status:
Licensed
g. Location Licensed:
Canada
h. Host Species for Licensed Use:
Human
i. Adjuvant: aluminum hydroxide vaccine adjuvant
j. Preservative:
2 phenoxy ethanol
k. Allergen:
Neomycin
l. Immunization Route
Intramuscular injection (i.m.)
m. Storage
Store at 2° to 8°C (35° to 46°F).
n . Approved Age for Licensed Use
16 years of age and older.
o. Description
Products: Polysaccharide + killed virus. Other components: Formaldehyde, Polysorbate 80.
60. Vivotif
a. Product Name:
Typhoid Vaccine Live Oral Ty21a
b. Tradename:
Vivotif
c. Manufacturer:
Berna Biotech, Ltd
d. Vaccine Ontology ID:
VO_0000120
e. CDC CVX code:
25
f. Type:
Live, attenuated vaccine
g. Status:
Licensed
h. Location Licensed:
USA (License # 1632)
i. Host Species for Licensed Use:
Human
j. Antigen
The antigen is live, attenuated strain Ty21a (Levine et al., 2007).


k. Preparation
Ty21a in enteric-coated capsules was created with 2 × 109–5 × 109 cfu of Ty21 (Levine et al., 2007).
l. Immunization Route
Oral
m. Storage
between 2 °C and 8 °C (35.6 °F– 46.4 °F).
n . Approved Age for Licensed Use
6 years of age and older.
o. Contraindication
Hypersensitivity to any component of the vaccine or the enteric-coated capsule (FDA: Vivotif).
p. Description
Indication: VIVOTIF is used for immunization of adults and children greater than 6 years of age against disease caused by Salmonella typhi (FDA: Vivotif).
q. Human Response
  • Vaccination Protocol: Two trials were completed with Ty21a: In the first, Participants were randomly allocated to receive, 1 week apart, either 2 doses of Ty21a in enteric-coated capsules, 1 dose of Ty21a and 1 dose of placebo, or 2 doses of placebo. In the second, participants were randomly allocated to receive 3 doses of vaccine in 1 of 2 different formulations or placebo. One formulation of Ty21a was enteric-coated capsules, and the other was a triad of gelatin capsules, with 1 containing lyophilized vaccine and the other 2 containing NaHCO3 buffer.
  • Persistence: Not noted.
  • Immune Response: Doses of Ty21a in capsules stimulates a form of antibody-dependent cellular cytotoxicity, in which IgA antibodies provide the specificity for cells to kill Salmonella organisms. Ty21a stimulates strong classical cell-mediated immune responses involving specific T cells that are believed to function through different effector mechanisms, such as production of IFN-gamma and other cytokines when stimulated by inactivated whole bacilli and purified S. Typhi flagella and the appearance of cytotoxic T cells that kill target cells infected with S. Typhi (Levine et al., 2007). Unless a complete immunization schedule is followed, an optimum immune response may not be achieved (FDA: Vivotif).
  • Side Effects: The most common side effects reported were: abdominal pain, nausea, headache, fever, diarrhea, vomiting and skin rash (FDA: Vivotif).
  • Efficacy: Results from clinical studies indicate that adults and children greater than 6 years of age may be protected against typhoid fever following the oral ingestion of 4 doses of VIVOTIF (FDA: Vivotif).
61. Vivotif L
a. Product Name:
Typhoid Vaccine Live Oral Attenuated Ty21a
b. Tradename:
Vivotif L
c. Manufacturer:
Berna Biotech
d. Vaccine Ontology ID:
VO_0011556
e. Type:
Live vaccine
f. Status:
Licensed
g. Location Licensed:
Canada
h. Host Species for Licensed Use:
Human
i. Immunization Route
Oral
j. Storage
Store between 2 and 8°C. Store in a dry place and protect from light.
k . Approved Age for Licensed Use
3 years of age and older.
l. Description
Products: Live virus. Other components: Lactose, Aspartame.
62. Zoosaloral H.
a. Tradename:
Zoosaloral H.
b. Type:
Live, attenuated vaccine
c. Antigen
The antigen is a strain of S. Typhimurium called Zoosaloral H.
d. Preparation
This live vaccine strain is derived by chemical mutagenesis from a virulent avian S. Typhimurium DT009 isolate. Chemical mutagenesis caused attenuation via adenine and histidine auxotrophy, but did not change the phage type of the vaccine strain (Frech et al., 1998).
e. Chicken Response
  • Vaccination Protocol: One-day-old SPF chickens were inoculated orally with 0.1 ml of a Zoosaloral culture such that the birds received between 6 x 108 and 1 x 109 cfu each (Methner et al., 1997).
  • Persistence: Not noted.
  • Challenge Protocol: The birds were challenged 24 h later orally with 0.1 ml of a culture of nalidixic acid-resistant Salmonella derivative diluted in PBS such that each bird received 104 cfu(Methner et al., 1997).
  • Efficacy: A significant amount of S. Typhimurium 421 was fund in the livers of the chickens after pretreatment and challenge (Methner et al., 1997).
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