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Streptococcus equi

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Disease
    3. Introduction
    4. Microbial Pathogenesis
    5. Host Ranges and Animal Models
    6. Host Protective Immunity
  2. Vaccine Related Pathogen Genes
    1. binding protein Eag (Protective antigen)
    2. cell surface protein (Protective antigen)
    3. collagen binding, ancillary pilus subunit Cne (Protective antigen)
    4. Fbp (Protective antigen)
    5. Fnz (Protective antigen)
    6. fnz (Protective antigen)
    7. Glyceraldehyde-3-phosphate dehydrogenase GAPDH (Protective antigen)
    8. HAP (Protective antigen)
    9. putative surface-anchored 5-nucleotidase (Protective antigen)
    10. sclC (Protective antigen)
    11. scpZ (Protective antigen)
    12. Sfs (Protective antigen)
    13. sfs (Protective antigen)
    14. spaZ (Protective antigen)
    15. surface-anchored protein (Protective antigen)
    16. szm (Protective antigen)
    17. SzP (Protective antigen)
    18. vicK (Virmugen)
  3. Vaccine Related Host Genes
    1. IgA
    2. IgG
  4. Vaccine Information
    1. Equilis StrepE
    2. S. equi Subunit CNE Protein Vaccine
    3. S. equi Subunit EAG, FNZ and SFS Protein Vaccine
    4. Streptococcus Equi Live Culture Vaccine (USDA: 1831.00)
    5. Streptococcus equi vicK mutant vaccine
  5. References
I. General Information
1. NCBI Taxonomy ID:
1336
2. Disease:
Strangles (also equine distemper)
3. Introduction
Strangles (also equine distemper) is a contagious, upper respiratory tract infection of horses and other equines caused by a bacterium, Streptococcus equi var equi. The disease is spread when the nasal discharge or material from the draining abscess contaminates pastures, barns, feed troughs, etc. Clinical signs include fever, heavy nasal discharge, and swollen or enlarged lymph nodes in the neck and throatlatch. Affected animals may also stop eating and have a dull affect. Mortality may be up to 1 in 10 horses affected with strangles, especially bastard strangles. For cases without complications, mortality is usually lower. The disease is very contagious and morbidity is high. Precautions to limit the spread of the illness are necessary and those affected are normally isolated. An isolation period of 6 weeks is usually necessary to ensure that the disease is not still incubating before ending the isolation (Wiki: Strangles).
4. Microbial Pathogenesis
S. equi enters via the mouth or nose and attaches to cells in the crypt of the tonsil and adjacent superficial lymphoid nodules. After a few hours, the organism is difficult to detect on the mucosal surface because it is translocated below the mucosa into the local lymphatics where it may be found in one or more of the lymph nodes that drain the pharyngeal/tonsillar region. Complement-derived chemotactic factors generated after interaction of C1 with bacterial peptidoglycan attract large numbers of PMN. The inability of PMN to phagocytose and kill the streptococci appears to be due to a combination of the hyaluronic acid capsule, antiphagocytic M protein, and a leukocidal toxin released by the organism. This culminates in accumulation of many extracellular streptococci in the form of long chains and large numbers of degenerating PMN. Final disposal of these organisms is dependent on lysis of the abscess capsule and evacuation of its contents (Timoney, 2004).
5. Host Ranges and Animal Models
S. equi primarily is a pathogen of equids, but can also cause disease in cattle, rabbits, swine, sheep and humans (MicrobeWiki: S. zooepidemicus). Mice can be used as a model of disease (Flock et al., 2006).
6. Host Protective Immunity
Most horses develop a solid immunity during recovery from strangles which persists for up to five years in about 75% of animals. Acquired resistance appears to be primarily humoral and mediated by antibodies to SeM and other as yet unidentified antigens unique to S. equi (Timoney, 2004).
1. binding protein Eag
  • Gene Name : binding protein Eag
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. equi 4047
  • VO ID : VO_0011211
  • NCBI Gene ID : 7696154
  • NCBI Protein GI : 225870123
  • Locus Tag : SEQ_0721
  • Genbank Accession : FM204883
  • Protein Accession : YP_002746070
  • Other Database IDs : CDD:144825
  • Taxonomy ID : 553482
  • Gene Starting Position : 711797
  • Gene Ending Position : 713086
  • Gene Strand (Orientation) : ?
  • Protein Name : Ig, alpha2-macroglobulin and albumin binding protein Eag
  • Protein pI : 4.57
  • Protein Weight : 41524.96
  • Protein Length : 429
  • Protein Note : B domain; pfam01378
  • DNA Sequence : Show Sequence
    >NC_012471.1:711797-713086 Streptococcus equi subsp. equi 4047, complete genome
    AATGGAAAAAAACAAAAAGGTATCCTACTTTTTACGTCAATCAGCTGTTGGTTTAGCTTCAGTGTCGGCA
    GCCTTTCTGGTTGGAACGACTTCAGTGGGTGCTTTAGACGCAGCAACAGTGTTAGAGCCTACAACAGCCT
    TCATTAGAGAAGCTGTTAGGGAAATCAATCAGCTGAGTGATGACTACGCTGACAATCAAGAGCTTCAGGC
    TGTTCTTGCTAATGCTGGAGTTGAGGCACTTGCTGCAGATACTGTTGATCAGGCTAAAGCAGCTCTTGAC
    AAAGCAAAGGCAGCTGTTGCTGGTGTTCAGCTTGATGAAGCAAGACGTGAGGCTTACAGAACAATCAATG
    CCTTAAGTGATCAGCACAAAAGCGATCAAAAGGTTCAGCTAGCTCTAGTTGCTGCAGCAGCTAAGGTGGC
    AGATGCTGCTTCAGTTGATCAAGTGAATGCAGCCATTAATGATGCTCATACAGCTATTGCGGACATTACA
    GGAGCAGCCTTGTTGGAGGCTAAAGAAGCTGCTATCAATGAACTAAAGCAGTATGGCATTAGTGATTACT
    ATGTGACCTTAATCAACAAAGCCAAAACTGTTGAAGGTGTCAATGCGCTTAAGGCAGAGATTTTATCAGC
    TCTACCGAGTTCTGAGGTCATTGACGCAGCAGAACTAACACCAGCCTTGACTAGCTATAAGCTTGTCATC
    AAGGGAGCAACTTTTTCAGGTGAAACAGCTACTAAGGCAGTAGACGCAGCTGTAGCTGAGCAGACCTTCA
    GAGACTATGCTAATAAAAATGGTGTAGACGGCGTTTGGGCTTATGATGCTGCCACAAAGACATTTACAGT
    CACTGAACAGCCTGTAGCTGAGACTATTGAGGCGGCAGAATTAACACCAGCTTTGACCACCTATAGGCTT
    GTTATTAAGGGGGTTACCTTCTCAGGTGAAACAGCTACCAAGGCAGTAGACGCAGCCACAGCCGAGCAGA
    CCTTCAGGCAATATGCCAATGACAATGGCGTTACTGGTGAATGGGCTTATGACGCTGCTACAAAGACATT
    TACAGTCACTGAGGCTTTAGAGGAGAGTCCAGCTGATCCAGAAAAGCCATCAGCTTCTCTTCCGTTGACA
    CCGCTTACACCAGCAACTAAGACAGCACCAGCTAAGCAAAAAGATAAGCAAAAGGCTAAGACTCTTCCAA
    CGACTGGTGAGAAGGCTAATCCATTCTTTACAGCAGCGGCTCTTGCCATTATGGCGAGTGCAGGTGCTTT
    AGCAGTGACTTCAAAGCGTCAGCAGGACTA
    
    
  • Protein Sequence : Show Sequence
    >YP_002746070.1 Ig, alpha2-macroglobulin and albumin binding protein Eag [Streptococcus equi subsp. equi 4047]
    MEKNKKVSYFLRQSAVGLASVSAAFLVGTTSVGALDAATVLEPTTAFIREAVREINQLSDDYADNQELQA
    VLANAGVEALAADTVDQAKAALDKAKAAVAGVQLDEARREAYRTINALSDQHKSDQKVQLALVAAAAKVA
    DAASVDQVNAAINDAHTAIADITGAALLEAKEAAINELKQYGISDYYVTLINKAKTVEGVNALKAEILSA
    LPSSEVIDAAELTPALTSYKLVIKGATFSGETATKAVDAAVAEQTFRDYANKNGVDGVWAYDAATKTFTV
    TEQPVAETIEAAELTPALTTYRLVIKGVTFSGETATKAVDAATAEQTFRQYANDNGVTGEWAYDAATKTF
    TVTEALEESPADPEKPSASLPLTPLTPATKTAPAKQKDKQKAKTLPTTGEKANPFFTAAALAIMASAGAL
    AVTSKRQQD
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Vaccination of mice with EAG (α2-macroglobulin, albumin, and immunoglobulin G [IgG] binding protein) as a single antigen with EtxB (as an adjuvant) induced protection in mice against bacterial challenge, but was less protective than at affording protection than immunization with FNZ, SFS, EAG, and EtxB (Flock et al., 2004).
    The antigens used were EAG (alpha2-macroglobulin, albumin, and IgG-binding protein), CNE (a collagen-binding protein), and SclC (a collagen-like protein). The vaccinated horses showed significantly reduced bacterial growth (p=0.02).(Waller et al., 2007)
  • Related Vaccine(s): S. equi Subunit EAG, FNZ and SFS Protein Vaccine
2. cell surface protein
  • Gene Name : cell surface protein
  • Sequence Strain (Species/Organism) : Streptococcus equi
  • NCBI Protein GI : WP_043026814
  • Other Database IDs : CDD:303769
    CDD:293650
    CDD:302792
    CDD:293879
    CDD:295219
    CDD:279132
  • Taxonomy ID : 1336
  • Protein Name : cell surface protein
  • Protein pI : 10.11
  • Protein Weight : 71332.09
  • Protein Length : 719
  • Protein Note : Guanylate-binding protein, C-terminal domain; cl20817
  • Protein Sequence : Show Sequence
    >WP_043026814.1 cell surface protein [Streptococcus equi]
    MITKKQRKQKRHTTIRRLCHQALAATLITGSLMGTGLLGNKVVYGDVKKDADNIKKVLEENKGKLSEELK
    KVTDFNTLLVLFSILSGDVSLSSSNLSSAITTFSDLVNSFNKQENNRNQANREALFSEISRRISALDEAT
    RNVMKTKQELKMTIQDLESEKLKKQLLNSELDKTREAYKLVIKTSNELEKKLNKATMDKKQAEEKAKKET
    AAKEKAEKDTKTAKQESEQANQRAEKAEAASKKAEDAAKKATEEANAAKEIAHQAQEKSKQLEQATQQAN
    QKAEQAEKAKKALETQKEKLEQEIKQAQKSKTEAEQKLKELQESSMKNDTAAQQLLKEKEELTTKLQDLQ
    KQAKEKTTEIEKLKQELEANKQNSGQLNQKEKELQEQLNKVQKELKQKEMELEKAKEQLKQEQKPHEGGG
    DSDASKARITELEKQVQTLTKEKESLSSTLESTKAQLSETQAKLSEAQKQLTATQEKLTTLEAEKAALQK
    QVDTISKQLSEARDLSEKEKAALQEQINKLKAEIEQKTKEIEALKQGMHSHQGQEKPKDPKTPETPKDPK
    TPEKNDQPQAPEKRSVPWTALTPAKPIDTTKAPKSSAPSPQTGAATPKKQLPATGDTATPSFFTAAAMAV
    LASVGVLNLSPRRKKNQNNR
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Fu et al., 2013)
3. collagen binding, ancillary pilus subunit Cne
  • Gene Name : collagen binding, ancillary pilus subunit Cne
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. equi 4047
  • VO ID : VO_0011213
  • NCBI Gene ID : 7695900
  • NCBI Protein GI : 225870316
  • Locus Tag : SEQ_0935
  • Genbank Accession : FM204883
  • Protein Accession : YP_002746263
  • Other Database IDs : CDD:283410
    CDD:212461
    CDD:273478
  • Taxonomy ID : 553482
  • Gene Starting Position : 914185
  • Gene Ending Position : 916158
  • Gene Strand (Orientation) : ?
  • Protein Name : collagen binding, ancillary pilus subunit Cne
  • Protein pI : 6.61
  • Protein Weight : 68135.68
  • Protein Length : 657
  • Protein Note : Collagen binding domain; pfam05737
  • DNA Sequence : Show Sequence
    >NC_012471.1:914185-916158 Streptococcus equi subsp. equi 4047, complete genome
    TTTGAAACAACTGACAAAGATCGTTAGTGTGGTCTTGTTGCTGGTCTTTACCCTTAGTGCTAGCCTGCAC
    AAGGTTCGGGCAACTAATCTTAGTGACAACATCACATCATTGACGGTTGCTTCTTCATCACTCCGAGATG
    GAGAGAGAACGACGGTAAAGGTTGCGTTTGATGACAAAAAACAGAAAATCAAGGCAGGGGATACGATAGA
    GGTCACCTGGCCTACAAGTGGTAATGTCTACATTCAGGGCTTTAATAAAACCATACCGCTTAATATTAGA
    GGGGTAGATGTTGGTACCTTGGAGGTCACGCTAGACAAGGCTGTTTTCACATTCAATCAAAATATTGAAA
    CAATGCATGATGTCTCTGGTTGGGGAGAGTTTGATATTACTGTTAGAAATGTGACACAAACCACCGCTGA
    AACATCAGGAACGACCACAGTAAAGGTAGGCAATCGCACTGCTACTATCACTGTTACTAAGCCTGAGGCA
    GGCACTGGTACCAGCTCATTTTATTATAAGACTGGTGATATGCAGCCCAATGATACTGAGCGTGTGAGAT
    GGTTCCTGCTGATTAACAACAACAAGGAATGGGTGGCCAATACTGTTACAGTCGAAGACGATATTCAAGG
    TGGTCAAACCTTGGATATGAGCAGCTTTGACATCACCGTATCTGGTTATCGTAACGAGCGCTTCGTTGGG
    GAAAACGCTCTGACAGAGTTTCATACAACATTTCCAAATTCTGTCATTACGGCAACAGATAATCACATTA
    GTGTGCGGTTAGATCAATATGATGCCTCACAAAACACTGTCAACATTGCTTATAAGACAAAGATAACGGA
    CTTTGACCAAAAAGAATTTGCCAACAACAGTAAAATCTGGTACCAGATTTTATACAAGGATCAGGTATCG
    GGTCAAGAGTCAAACCACCAAGTAGCCAATATCAATGCTAACGGCGGGGTTGATGGCAGTCGCTATACCA
    GCTTTACTGTCAAGAAAATTTGGAATGACAAGGAAAATCAAGACGGTAAGCGTCCAAAGACTATTACTGT
    TCAGCTTTACGCCAATGATCAGAAAGTTAATGATAAGACCATTGAATTGAGTGATACTAATAGCTGGCAA
    GCAAGTTTTGGTAAGCTGGATAAGTATGACAGTCAGAACCAAAAAATTACCTACAGTGTCAAGGAAGTGA
    TGGTTCCTGTTGGCTACCAATCGCAGGTTGAGGGGGATAGTGGAGTAGGATTTACCATTACCAACACCTA
    TACACCAGAGGTCATTAGCATTACCGGTCAAAAAACTTGGGACGACAGGGAAAACCAAGACGGTAAACGT
    CCTAAGGAGATTACGGTTCGTTTATTGGCAAATGACGCTGCAACTGACAAGGTAGCAACTGCTTCAGAGC
    AAACCGGCTGGAAGTATACATTTACCAATCTACCGAAATACAAAGATGGTAAACAGATCACCTACACGAT
    CCAAGAGGACCCTGTGGCAGATTACACCACAACCATTCAGGGATTTGATATTACCAATCATCATGAGGTA
    GCCTTGACCAGCCTAAAGGTCATCAAGGTTTGGAATGATAAGGACGATTATTACCATAAACGTCCCAAGG
    AGATTACCATTTTGCTAAAGGCAGATGGCAAGGTGATTCGTGAACATCAGATGACACCGGATCAGCAAGG
    AAAATGGGAATACACCTTTGACCAGCTGCCGGTCTATCAGACAGGCAAGAAAATCAGCTACAGCATTGAG
    GAAAAACAGGTTGCTGGCTATCAAGCCCCTGTCTATGAGGTTGATGAAGGCTTGAAGCAGGTCACTGTAA
    CCAACACCCTTAACCCAAGCTACAAGCTGCCTGACACCGGAGGACAAGGAGTGAAATGGTACCTGTTAAT
    CGGTGGCGGTTTTATCATCGTCGCAATCCTTGTACTGATCAGCCTTTATCAAAAACACAAGCGCCATAAC
    ATGTCAAAACCATA
    
    
  • Protein Sequence : Show Sequence
    >YP_002746263.1 collagen binding, ancillary pilus subunit Cne [Streptococcus equi subsp. equi 4047]
    MKQLTKIVSVVLLLVFTLSASLHKVRATNLSDNITSLTVASSSLRDGERTTVKVAFDDKKQKIKAGDTIE
    VTWPTSGNVYIQGFNKTIPLNIRGVDVGTLEVTLDKAVFTFNQNIETMHDVSGWGEFDITVRNVTQTTAE
    TSGTTTVKVGNRTATITVTKPEAGTGTSSFYYKTGDMQPNDTERVRWFLLINNNKEWVANTVTVEDDIQG
    GQTLDMSSFDITVSGYRNERFVGENALTEFHTTFPNSVITATDNHISVRLDQYDASQNTVNIAYKTKITD
    FDQKEFANNSKIWYQILYKDQVSGQESNHQVANINANGGVDGSRYTSFTVKKIWNDKENQDGKRPKTITV
    QLYANDQKVNDKTIELSDTNSWQASFGKLDKYDSQNQKITYSVKEVMVPVGYQSQVEGDSGVGFTITNTY
    TPEVISITGQKTWDDRENQDGKRPKEITVRLLANDAATDKVATASEQTGWKYTFTNLPKYKDGKQITYTI
    QEDPVADYTTTIQGFDITNHHEVALTSLKVIKVWNDKDDYYHKRPKEITILLKADGKVIREHQMTPDQQG
    KWEYTFDQLPVYQTGKKISYSIEEKQVAGYQAPVYEVDEGLKQVTVTNTLNPSYKLPDTGGQGVKWYLLI
    GGGFIIVAILVLISLYQKHKRHNMSKP
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice were immunised i.n. with collagen-binding protein (CNE) and were subsequently subjected to infection with S. equi subsp. equi. A control group of mice was given only adjuvant. The CNE vaccinated group had a delayed onset of infection compared to the control group. Immunization with CNE led to significant protection from infection (Flock et al., 2006).
    The antigens used were EAG (alpha2-macroglobulin, albumin, and IgG-binding protein), CNE (a collagen-binding protein), and SclC (a collagen-like protein). The vaccinated horses showed significantly reduced bacterial growth (p=0.02).(Waller et al., 2007)
  • Related Vaccine(s): S. equi Subunit CNE Protein Vaccine
4. Fbp
  • Gene Name : Fbp
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. equi 4047
  • VO ID : VO_0011216
  • NCBI Gene ID : 7697172
  • NCBI Protein GI : 225871286
  • Locus Tag : SEQ_2017
  • Genbank Accession : FM204883
  • Protein Accession : YP_002747233
  • Taxonomy ID : 553482
  • Gene Starting Position : 2043360
  • Gene Ending Position : 2044964
  • Gene Strand (Orientation) : -
  • Protein Name : antiphagocytic cell surface-anchored fibrinogen-and IgG Fc-binding protein SeM
  • Protein pI : 9.36
  • Protein Weight : 55929.26
  • Protein Length : 534
  • Protein Note : Previously sequenced as Streptococcus equi fibrinogen-binding protein Fbp UniProt: AF012927 (534 aa" target="_blank">O68165 (EMBL: AF012927 (534 aa) blastp scores: E()=0.0
  • DNA Sequence : Show Sequence
    >NC_012471.1:2043360-2044964 Streptococcus equi subsp. equi 4047, complete genome
    CTTAGTTTTCTTTGCGTTTAGGAGACACCACAGCCATACCAGCACCAGCGATGACAGTAAGAGCTGCAAT
    AGTGAAGAATGGGTTAGCAGACTCACCAGTAGATGGCAATTGACCTGCTTTAGCAGTTGTAGTGCCTGGG
    TTTGTTTGTGAAGCACGACCACCTTGTGACAATGACTTAATCCTGTCAGCTAGCTCTTGCTTCAAGCGAT
    TGATTTCTGCTTGAAGATCTGCATTTGCATGGTCTTTCTTCTCAAGCATTTCTTTAAGTTTTGAAAGTTC
    CTCAGCATGAGCTTTTTTGAGCTCTTCAATCTTAGCTTCAAGCGCTTCTTTTTCCTTCTTAACGGCTTCA
    AGCTCTTCTTCTTTCTTAGCTAATTGTACTTTAAGATCTGCAACCTCAGCTTTAGCAGCTTCAACTTCTT
    TTTCAAGCTCTGCTACCTTAGCATCTGATGCTGCTTTTTGTTTTTCAAGCTCTGCTACATTAGCTTGTGA
    TTCTACTAAAGCCTTATCAGAAGCATCTTTTTGTTTTTGTAGCTCAGCAATTTCACGTTTGCCTGATTCA
    ATCAAACGCTCTGAAACAGCAAGCTCTGTCTTAGCTTTTTCTAGCTCTGTTTGAAGTGATGCAATGGTGT
    CATTTGCAGCTGCAAGCTCTGCATCAGCCTTAGCCTTATCAGCAGTTAATTGATCAATTTCTGCTAATTT
    TTGAGCCATTTCACGTTTTGCAGACTCTACCAAGCGGTCTAGACTACCAACGTTTTCCATTAACTTTTCA
    GCTTCTGCTGTTTTAGCTGCCAATTTATCTGCTAAATCTTTTGATACTGCAAAAGCTTTATCACGACTTG
    CTTCAGCACTACTTAGTTTATCTGCTAAATCTTTTGATACTGCAAAGGCTTTATCACGACTTGCTTCAGC
    ATTGCTTAGCTTATCTGCTAATTCCTTAGTCATCTTAGCTGCTTTTTCTTTTTCTTTTGCAAGTTCTGTT
    TTTAGTTTATTATACTCTTTTGTTGATCTTGATAAAGTTTTGTTGAATGATTCGGTAAGAGCTTGACGCT
    CATCTTTAGCTTTTTGTAGTTCTTGTTCTTTTTCCTTCTCTAGATTTCGAAGTTGGGAATCTCTTTCTCT
    AAGCATTTCTTGTTGAGATTTAATAGCTGCAAGTTTAGCCTCAAGAAGTTTTGCATAACTATCTCCATCA
    GCAATCCGCTTTTCAATTTCATCTACAAGTAATGAAGCCAAACTTAATTGTCTTCTATCCCCATCAGGTT
    TATCATTTAACATCGATGAAAGGTGCACCAATAAATTGTAATAATCATCTCTACCATACGCAGCCCTTGC
    TGAATCAAGACCTCTCAATAATGCCTGTAAATCCCCAACAGAGGCGCCTTTTAGAAGATTTCGAACTTTT
    TGGGCTGATGAGGCATCTCTACTTATGGCTATTTCGCTTAATCTATTTTTTAAATCACGCGATAATCTTG
    GAGTCGCCGTACGACTAACCTCAGAGTTCGCTTTTACAGTTGTCCCTCCCAACACACTTGTTGCAACTAA
    TACTGATGCTGCACCGGCACTTAGTTTTCTGATGCTAAATTTTTGCTTGTTATTTCTCAAAAACA
    
    
  • Protein Sequence : Show Sequence
    >YP_002747233.1 antiphagocytic cell surface-anchored fibrinogen-and IgG Fc-binding protein SeM [Streptococcus equi subsp. equi 4047]
    MFLRNNKQKFSIRKLSAGAASVLVATSVLGGTTVKANSEVSRTATPRLSRDLKNRLSEIAISRDASSAQK
    VRNLLKGASVGDLQALLRGLDSARAAYGRDDYYNLLVHLSSMLNDKPDGDRRQLSLASLLVDEIEKRIAD
    GDSYAKLLEAKLAAIKSQQEMLRERDSQLRNLEKEKEQELQKAKDERQALTESFNKTLSRSTKEYNKLKT
    ELAKEKEKAAKMTKELADKLSNAEASRDKAFAVSKDLADKLSSAEASRDKAFAVSKDLADKLAAKTAEAE
    KLMENVGSLDRLVESAKREMAQKLAEIDQLTADKAKADAELAAANDTIASLQTELEKAKTELAVSERLIE
    SGKREIAELQKQKDASDKALVESQANVAELEKQKAASDAKVAELEKEVEAAKAEVADLKVQLAKKEEELE
    AVKKEKEALEAKIEELKKAHAEELSKLKEMLEKKDHANADLQAEINRLKQELADRIKSLSQGGRASQTNP
    GTTTAKAGQLPSTGESANPFFTIAALTVIAGAGMAVVSPKRKEN
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : The fibrinogen-binding protein (FgBP) reacted with serum taken from horses recovering from strangles and protected mice against lethal challenge from S. equi subsp. equi (Meehan et al., 1998).
5. Fnz
  • Gene Name : Fnz
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. zooepidemicus
  • VO ID : VO_0011214
  • NCBI Gene ID : 7695217
  • NCBI Protein GI : 754170878
  • Locus Tag : SZO_RS08670
  • Genbank Accession : FM204884
  • Protein Accession : WP_041790586
  • Taxonomy ID : 40041
  • Gene Starting Position : 1860617
  • Gene Ending Position : 1862326
  • Gene Strand (Orientation) : +
  • Protein Name : thioester-forming surface-anchored protein
  • Protein pI : 4.75
  • Protein Weight : 58938.07
  • Protein Length : 569
  • Protein Note : Ortholog of S. equi 4047 (FM204883) SEQ0375
  • DNA Sequence : Show Sequence
    >NC_012470.1:1860617-1862326 Streptococcus equi subsp. zooepidemicus H70, complete genome
    TATGGAGAGAAGAGAGTTGAAAACAAAATCATTTAGAAAGGTACTGACGACGTCAGCTACCTGTATTGTG
    CTGGCAACAAGCTTTGCCGGAGGAACCCTACGCGTCTGGGCAGAGCAGCTTTATTATGGGTGGGATGATG
    GAACGAGACATGATTCACCCTCCTTTTTATATGTAACGCCTACTACTGAAAGTCAAAGAAGCCCCAAAAC
    TATTGTATACTGTTTTAATCGAAACAATTATTGGCCGGATAATTGGGAAACCCATGTCTTAAAACCAGAT
    AATCTACCTACTTATAATAAATTAACAGGAACTGCGAAGTTATTTCAAAATAAAGCTACCAAACCAAGAT
    CGGTAGCAGATATCGAGAAGTCCTTAGTTTCTGTCCTAAGTAAAGGGTATCCAACAAGTAATCTCTCTCA
    ATTTGGCATCAGTAATGAGTCAGCTAGAATAGTGACACAATTAGCCATTTGGTATTTTAGTGATAGTGTG
    AACGATCCTGAAAGCGTACTTAGACAAAGATTAGGGGGCAATGAATCTAAAGCATTAAAATACCTTATTG
    AGGTTGGTGAAAAACCACAATTGAGCGAAGCTGAGCAAAAGCAAACGTTAGATATATACGTTTACAACTC
    AGGAGGTTATCGTAGAACAGGTTACCAAAATCTTCTCGGCTCTACCTTAATTCCTAAAACACCAGAAAAG
    CCTTCTTCTGAATCTGTAACTCAGAGTAACCTTATTCTACTTGAACAGGAGAATCATTCTGGTACTATTT
    CAGGTGGTTCAGGAAATACTGAAACTGAGGAGAATACTCCTCATCTAATGGGGATAGGTGGCGGTCTAGC
    TGGTGAATCAGGAGAAAACACACCTAAACCAGGGGAAACTGGTGGGCACTCCCCTATAGTTGAAGAAAAC
    TACGGTAGTACTGAAGAATTTCATGGGAATTCAGAAATTATTAGTGAAACCGAAGATACTAATCCTGGTA
    TCATTTTAGGTGGCTCAGGAAATGTCGAAACACATGAAGATACTAGGAATCCTCATCTGATGGGGCTTGG
    CGGTGGTCTAGCTGGCGAATCAGGAGAAACGACACCTAAACCAGGACAGACTGGTGGTCATGGACCAATC
    ATCGAGACAACAGAGGACACACAAAAAGGCATGTCTGGGCAATCTGGCGGCACTATCGAGTCAGAAAACA
    CCAAAAAGCCTGAGGTCATGATTGGTGGGCAGGGACAAACCATCGAAACAACAGAGGACACACAAAAAGG
    CATGTCTGGGCAATCTGGCGGTACTATCGAGTCAGAAGACACTAAAAAACCTGAGGTCATGATTGGCGGT
    CAGGGACAAATCATCGACTTCTCTGAAAACACGCAATCAGGTATGTCTGGACAGTCTGGAGACACTACGG
    TCATTGAGGATACCAAGAAGTCTGAGATAATCATTGGTGGGCAAGGACAAATCATCGACTTCTCTGAGGA
    TACTCAGCCGGGTATGTCTGGGCAATCTGGAGGCACCACAATTGTCGAAGACACCAAGAAGCCGACACCT
    AAGCCTAAACCTGCACCTGCGCCAATTGTTAATGACGAAAAACCTAACAAAGGCACTCAGCTCCCACAGA
    CAAATGATATGAAGCAACTCACCCTAAGCATCATCGGTGCAATGTCAATGCTGCTTGTCCTATGTCTGTC
    TCTATTCAAGCGACCATCTAAAAAAGACTA
    
    
  • Protein Sequence : Show Sequence
    >WP_041790586.1 thioester-forming surface-anchored protein [Streptococcus equi]
    MERRELKTKSFRKVLTTSATCIVLATSFAGGTLRVWAEQLYYGWDDGTRHDSPSFLYVTPTTESQRSPKT
    IVYCFNRNNYWPDNWETHVLKPDNLPTYNKLTGTAKLFQNKATKPRSVADIEKSLVSVLSKGYPTSNLSQ
    FGISNESARIVTQLAIWYFSDSVNDPESVLRQRLGGNESKALKYLIEVGEKPQLSEAEQKQTLDIYVYNS
    GGYRRTGYQNLLGSTLIPKTPEKPSSESVTQSNLILLEQENHSGTISGGSGNTETEENTPHLMGIGGGLA
    GESGENTPKPGETGGHSPIVEENYGSTEEFHGNSEIISETEDTNPGIILGGSGNVETHEDTRNPHLMGLG
    GGLAGESGETTPKPGQTGGHGPIIETTEDTQKGMSGQSGGTIESENTKKPEVMIGGQGQTIETTEDTQKG
    MSGQSGGTIESEDTKKPEVMIGGQGQIIDFSENTQSGMSGQSGDTTVIEDTKKSEIIIGGQGQIIDFSED
    TQPGMSGQSGGTTIVEDTKKPTPKPKPAPAPIVNDEKPNKGTQLPQTNDMKQLTLSIIGAMSMLLVLCLS
    LFKRPSKKD
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Parts of the three recombinant proteins (FNZ (cell surface-bound fibronectin binding protein), SFS (secreted fibronectin binding protein), and EAG (alpha2-macroglobulin, albumin, and immunoglobulin G [IgG] binding protein)) were used to immunize mice, either subcutaneously or intranasally, prior to nasal challenge with S. equi subsp. equi. The adjuvant used was EtxB, a recombinant form of the B subunit of Escherichia coli heat-labile enterotoxin. It was shown that nasal colonization of S. equi subsp. equi and weight loss due to infection were significantly reduced after vaccination compared with a mock-vaccinated control group. This effect was more pronounced after intranasal vaccination than after subcutaneous vaccination; nearly complete eradication of nasal colonization was obtained after intranasal vaccination (P < 0.001) (Flock et al., 2004).
  • Related Vaccine(s): S. equi Subunit EAG, FNZ and SFS Protein Vaccine
6. fnz
  • Gene Name : fnz
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. zooepidemicus MGCS10565]
  • NCBI Protein GI : ACG61689
  • Other Database IDs : CDD:285533
    CDD:274864
    CDD:281046
  • Taxonomy ID : 552526
  • Protein Name : fibronectin-binding protein Fnz
  • Protein pI : 4.97
  • Protein Weight : 60736.07
  • Protein Length : 682
  • Protein Note : Fibronectin-binding protein signal sequence; pfam08341
  • Protein Sequence : Show Sequence
    >ACG61689.1 fibronectin-binding protein Fnz [Streptococcus equi subsp. zooepidemicus MGCS10565]
    MERRELKTKSFRKVLTTSATCIVLATSFAGGTLRVWADKYYGWNDGTRRDSPFFLYVSPQNDPKRELKDE
    CIVYCFNRDKQWPETWGVNRTFSQVQSEVTFKLPLYEKKKGSDEVFKNNAPRFRGTVKNVTAALVAVLKS
    GYPTVKSINHLDAEKSRKVTQLAIWYFSDSENDPDTYLQPSEKLTQEEKTAFNSLVEEGKKAGETLSSTG
    DYTLDIYITTDKNYAQKQYQNLLGSHVIPKKDDTPNLHGSGPKLNGEIGGKTELPNSIGGESRYPNDEKG
    EAGPPATPQKPNDPLQGLEGGNSPIVEQNYGSTEGYHGQSGILEETEDTNPPGIILGGSGNVETHEDTRN
    PHLMGIGGGLAGESGETTPKPGQTGGHGPIIEITEDTQKGMSGQSGGTIESENTKKPEVLIGGQGQTIET
    TEDTQKGMSGNSGGTIESEDTKKPEVLIGGQGQIIDFSENTQSGMSGQSGDTTVIEDTKKSEIIIGGQGQ
    IIDFSEDTQPGMSGQSGGTTIVEDTKKPTPKPKPAPAPIVNDEKPNKGTHLPQTSEFKQLTLSIIGAMSM
    LLVLCLSLFKRPSKKD
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Flock et al., 2004)
7. Glyceraldehyde-3-phosphate dehydrogenase GAPDH
  • Gene Name : Glyceraldehyde-3-phosphate dehydrogenase GAPDH
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. zooepidemicus SzS31A1
  • NCBI Protein GI : EQB24294
  • Taxonomy ID : 1352602
  • Protein Name : glyceraldehyde-3-phosphate dehydrogenase
  • Protein pI : 4.97
  • Protein Weight : 32411.54
  • Protein Length : 445
  • Protein Sequence : Show Sequence
    >EQB24294.1 glyceraldehyde-3-phosphate dehydrogenase [Streptococcus equi subsp. zooepidemicus SzS31A1]
    MVVKVGINGFGRIGRLAFRLIQNVEGVEVTRINDLTDPAMLAHLLKYDSTQGRFDGDVEVVEGGFNVNGK
    FVKVSAEANPENIDWASQEVDIVLEATGFFASKAGAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHEIL
    DGTETVISGASCTTNCLAPMAKVLQDQFGVVEGLMTTIHGYTGDQMTLDAPHRKGDFRRARAAAVNIVPN
    STGAAKAIGLVIPELNGKLDGAAQRVPVPTGSVTELVSVLEKEVTADEVNAAMKAAANDSFGYTEDQIVS
    SDVVGIHYGSLFDATQTKVLTVDGKQLVKTVSWYDNEMSYTAQLVRTLEYFAKIAK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Fu et al., 2013)
8. HAP
  • Gene Name : HAP
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. equi 4047
  • NCBI Protein GI : 4336671
  • Other Database IDs : CDD:226636
    CDD:173869
  • Taxonomy ID : 553482
  • Gene Strand (Orientation) : ?
  • Protein Name : hyaluronate-associated protein precursor
  • Protein pI : 5.08
  • Protein Weight : 53754.78
  • Protein Length : 622
  • Protein Note : ABC-type oligopeptide transport system, periplasmic component [Amino acid transport and metabolism]; COG4166
  • Protein Sequence : Show Sequence
    >AAD17886.1 hyaluronate-associated protein precursor [Streptococcus equi subsp. equi 4047]
    MTVLGTKACKRLGLAAVTLASVAALMACGNKQSASTDKKSEINWYTPTEIITLDISKNTDTYSALAIGNS
    GSNLLRADAKGKLQPDLAEKVDVSEDGLTYTATLRDGLKWSDGSDLTAEDFVYSWQRMVDPKTASEYAYL
    ATESHLKNAEDINSGKNPDLDSLGVKADGNKVIFTLTEPAPQFKSLLSFSNFVPQKESFVKDAGKDYGTT
    SEKQIYSGPYIVKDWNGTSGTFKLVKNKNYWDAKNVKTETVNVQTVKKPDTAVQMYKQGKLDFANISGTS
    AIYNANKKHKDVVPVLEATTAYIVYNQTGAIEGLNSLKIRQALNLATDRKGIVSAAVDTGSKPATALVPT
    GLAKLSDGTDLTEHVAPGYKYDDKEAAKLFKEGLAELGKDALTITITADADAPAAKSAVDYIKETWETAL
    PGLTVEEKFVPFKQRLEDTKNQNFEVAVVLWGGDYPKGSTFYGLFKSGSAYNYGKFTNADYDAAYNKALT
    TDALNTDAAADDYKAAEKALYDNALYNPLYLP
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Immunization with HAP-GST significantly reduced rhinitis in Balb/C mice challenged nasally with S. equi and significantly increased survival time and clearance of bacteria in CBA/CA mice challenged intraperitoneally with S. zooepidemicus (Chanter et al., 1999).
9. putative surface-anchored 5-nucleotidase
  • Gene Name : putative surface-anchored 5-nucleotidase
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. Zooepidemicus
  • NCBI Protein GI : CAW99100
  • Other Database IDs : CDD:223808
    CDD:301300
    CDD:277317
    CDD:280945
    EnsemblGenomes-Gn:SZO_08800
    EnsemblGenomes-Tr:CAW99100
    GOA:C0MEK3
    InterPro: IPR004843
    InterPro: IPR006146
    InterPro: IPR006179
    InterPro: IPR008334
    InterPro: IPR019931
    InterPro: IPR029052
    UniProtKB/TrEMBL: C0MEK3
  • Taxonomy ID : 40041
  • Protein Name : putative surface-anchored 5'-nucleotidase
  • Protein pI : 4.64
  • Protein Weight : 66099.44
  • Protein Length : 775
  • Protein Note : Signal peptide predicted for SZO08800 by SignalP 2.0 HMM (Signal peptide probability 1.000) with cleavage site probability 0.986 between residues 27 and 28
  • Protein Sequence : Show Sequence
    >CAW99100.1 putative surface-anchored 5'-nucleotidase [Streptococcus equi subsp. zooepidemicus]
    MKKHFVLKSSVLGVFAGVSMLVTGVQADQVDVQFLGVNDFHGALDTTGSAYMPEGKVSNAGTAAQLAAYL
    DDAEADFKQTSPDGTSIRVQAGDMVGASPANSGLLQDEPTVKVFNQMGFEYGTLGNHEFDEGLAEFNRIM
    TGTAPAEDSAINDITKQYEHEASNQTLVIANVVDKKTNTIPFGWKPYAVKEIPVNDKSVKVGFIGVVTTE
    IPNLVLKQNYEQYDYLDSAETIVKYAKELQEQQVNAIVVLAHIPATSDNGVVHDEMAAVMEKVNQLYPEH
    SVDIVFAGHNHQYTNGTIGTTRVVQAVSQGKAYADVRGTLDTDLNDFVATPEAKVIAVAPGIKTGNADIQ
    AIVDQANDIVKTVTELKIGTAATSSTISKTENEDKESPVGNLITTAQLTIGRKTFPHVDFAMTNNGGIRA
    DLVVKEDQTITWGAAQAVQPFGNILQVVQMTGQQIYDVLNQQYDEDQKYFLQMSGLKYTYTANEANDPST
    PFKVVKAYKDNGEEIDPAAQYTLIINDFLYGGGDGFSVFKDAKLLGAINPDTEVFIEYIKDLEAAGKTVS
    AGINGVKTYVTASLEGSTQSDHTGKHEIINRVFKNREGKVLSTELVSDLFTPAQNANPATSIKPLAAKKQ
    ANAALPATGTSQHSAAAMTILGLLTLTGTSLFKKKEGQ
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Wei et al., 2012)
10. sclC
  • Gene Name : sclC
  • Sequence Strain (Species/Organism) : Streptococcus equi
  • NCBI Protein GI : AAV70833
  • Other Database IDs : CDD:333130
    CDD:307064
  • Taxonomy ID : 148942
  • Protein Name : collagen-like protein C
  • Protein pI : 9.94
  • Protein Weight : 30137.67
  • Protein Length : 377
  • Protein Note : group: C
  • Protein Sequence : Show Sequence
    >AAV70833.1 collagen-like protein C [Streptococcus equi subsp. equi]
    MTNKTKRTGLVRKYGACSAAIALAALASLGAGKAVKADQPAALKYPEPRDYFLHTREGDVIYDEDIKRYF
    EDLEAYLTARLGGIDKKVEEAAQKPGIPGPTGPQGPKGDKGDPGAPGERGPAGPKGDTGEAGPRGEQGPA
    GQAGERGPKGDPGAPGPKGEKGDTGAVGPKGEKGDTGATGPKGDKGERGEKGEQGQRGEKGEQGQRGEKG
    EQKPKGDQGKDTKPSAPKAPEKAPAPKAPKASEQSSNPKAPAPKSAPSKSAAPTGQKAALPATGEINHPF
    FTLAALSVIASVGVLTLKGKKD
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Waller et al., 2007)
11. scpZ
  • Gene Name : scpZ
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. zooepidemicus MGCS10565]
  • NCBI Protein GI : ACG62304
  • Other Database IDs : CDD:173801
    CDD:278510
    CDD:239048
    CDD:283851
    CDD:304423
  • Taxonomy ID : 552526
  • Protein Name : C5a peptidase precursor ScpZ
  • Protein pI : 4.77
  • Protein Weight : 115524.31
  • Protein Length : 1234
  • Protein Note : Peptidase S8 family domain in Streptococcal C5a peptidases; cd07475
  • Protein Sequence : Show Sequence
    >ACG62304.1 C5a peptidase precursor ScpZ [Streptococcus equi subsp. zooepidemicus MGCS10565]
    MEVLLVTKKYHSKKSLLCQLTLATTSILLLHSQTVFAEEQQGSDASVSQASPVVAAEATATGDDSQAAAS
    NQAASETEAKTEKEISAFAEGTESTAPTTLSVHDPANAQPLQEQTQKGSGAVIAVIDVSFDPEHDAFKLD
    SDLKNNGTIPAKEAFLAKKQDQHITYGKWYNEKIIFAYDYTKQSDDIGGLTADNGHGNHTAAIAAGNGKT
    PAGNGLRVEGIAPNASLMLMKVTGAPERDQFAKSYAKAITDAVNLGATVISMSFGKTADSLSTVHEDVKK
    AMAYAQEKGVLLVAGAGNESAVGMGTREPLAANPDFGTLNSPAIFEEVISVAASNPVYAISQSVNAKTAD
    GSQKLAIFMSEGDSFDADQDYFVADAKFGEASHFNGSDVKGKVALIERGGSLSFYQKIENAVKAGASGVI
    IYNNNALEGSFTIEKASIPKEARIPVGFMSYKDAQALKAGHSFTFNKAYEKMPSNAGGRVISQSSWGVTA
    EGRIKPDISAPGVNVYSAVHGNQYDYKTGTSMSTPMVSGLVAMLHKAYKEKFPELSAQELSQLVRAVLMS
    SARTLYSAENKAYISPRQQGAGEVDGQKALAASYYLTDQKQNPKINLGNITDEFVINLNVNALSKNQGPK
    KLYFQVNLITDQTKDGHFTLQPKALKDSEWQEINITEDQQHIQVSVDAKAYAAELLKAMPNGYFLEGFVR
    FTDNLDTKEELMSIPFSGFRGDFANLPALDTPIYETLEQGAFYQKLEQDPETGKYILPEKFSKLTALIGQ
    VSPYFLSQDTKNGMVNELGPEAERYIILGTQESADQKDLIAKEADRTFLISPNDDGNKDFILFQGVFLRS
    VKDIKAQVLDHQGQVVWESDVATAQKYYQEGVVLPYRFEHTKWSGKDANDQPVADGSYTYRVWYTPIADG
    AEAQKQDFKVQVKTSLPELPKSASYDESTRTLKIDASNFPAYRIQVGHIVEIGEGEEADIVVNYFPMAED
    GTVIIPKTVTSELSGEEVEVDMAALKVIVEDEFGNFNAIALADLLKQKEPEIKDDKQPEPETPMPEQPKG
    NDSSKEQQSPKGNNSGSKQPLVMKQDSAVKPMTSSPSHELPKTSEKQGSSLLAGLGMLLASLSLGLFKKK
    SDN
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Wei et al., 2013)
12. Sfs
  • Gene Name : Sfs
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. equi 4047
  • VO ID : VO_0011215
  • NCBI Gene ID : 7696331
  • NCBI Protein GI : 225869898
  • Locus Tag : SEQ_0466
  • Genbank Accession : FM204883
  • Protein Accession : YP_002745845
  • Taxonomy ID : 553482
  • Gene Starting Position : 452509
  • Gene Ending Position : 453624
  • Gene Strand (Orientation) : ?
  • Protein Name : fibronectin-binding protein Sfs
  • Protein pI : 8.97
  • Protein Weight : 39068.51
  • Protein Length : 371
  • Protein Note : Previously sequenced as Streptococcus equi fibronectin-binding protein Sfs UniProt: AF136451 (371 aa" target="_blank">Q9X6F4 (EMBL: AF136451 (371 aa) fasta scores: E()=1.8e-153, 100.000% id in 371 aa
  • DNA Sequence : Show Sequence
    >NC_012471.1:452509-453624 Streptococcus equi subsp. equi 4047, complete genome
    TATGAGAAAAACAGAAGGACGTTTTCGCACATGGAAGTCCAAAAAACAATGGCTATTTGCCGGTGCAGTG
    GTGACCAGCTTGCTGCTGGGAGCTGCACTTGTCTTTGGAGGTTTATTAGGAAGTCTTGGTGGCTCATCCC
    ATCAAGCGAGGCCAAAGGAGCAGCCAGTCAGCTCGATTGGAGATGACGATAAGTCGCACAAGAGCTCATC
    AGATTCAATGGTCAGTCGGCCACCGAAAAAGGATAACTTGCAGCCTAAGCCTTCAGATCAGCCTACTAAT
    CATCAGCATCAGGCTACTAGCCCGTCCCAGCCGACAGCAAAGAGCTCAGGTCATCATGGGAATCAACCTC
    AGTCTCTCAGTGTGAACAGCCAAGGAAATAGTAGTGGACAGGCCTCAGAGCCTCAGGCTATTCCTAATCA
    AGGGCCTTCCCAGCCACTGGGGCTGAGAGGAGGTAACAGCTCTGGTTCAGGTCATCACCATCAGCCACAA
    GGAAAGCCACAGCACTTAGATCTAGGTAAGGATAATTCTAGCCCGCAGCCTCAACCAAAGCCTCAGGGCA
    ATAGTCCCAAGCTGCCAGAAAAAGGCTTGAATGGTGAAAATCAGAAGGAACCGGAGCAAGGTGAACGAGG
    TGAGGCTGGTCCCCCACTTTCAGGGTTGAGTGGTAATAATCAAGGCCGTCCTTCGCTTCCAGGCTTGAAT
    GGTGAGAATCAGAAGGAACCAGAGCAAGGTGAACGAGGTGAAGCCGGTCCCCCATCAACTCCGAATTTAG
    AAGGAAATAATCGTAAAAATCCTTTAAAAGGATTAGATGGAGAGAATAAGCCAAAGGAAGATTTAGACGG
    TAAAGGCTTAAGCGGAGAAAATGATGAATCACCAAAACTTAAAGACGAACACCCCTACAATCATGGTCGT
    CGTGATGGTTACCGAGTTGGCTATGAGGATGGATATGGTGGCAAAAAGCACAAAGGAGATTATCCTAAGC
    GGTTTGATGAGTCTAGTCCAAAGGAATATAATGACTATAGTCAAGGGTATAATGATAATTATGGAAATGG
    CTATTTAGATGGTCTAGCTGATAGAGGTGGTAAGAGAGGATACGGCTATTCTTACAATCCCGACTA
    
    
  • Protein Sequence : Show Sequence
    >YP_002745845.1 fibronectin-binding protein Sfs [Streptococcus equi subsp. equi 4047]
    MRKTEGRFRTWKSKKQWLFAGAVVTSLLLGAALVFGGLLGSLGGSSHQARPKEQPVSSIGDDDKSHKSSS
    DSMVSRPPKKDNLQPKPSDQPTNHQHQATSPSQPTAKSSGHHGNQPQSLSVNSQGNSSGQASEPQAIPNQ
    GPSQPLGLRGGNSSGSGHHHQPQGKPQHLDLGKDNSSPQPQPKPQGNSPKLPEKGLNGENQKEPEQGERG
    EAGPPLSGLSGNNQGRPSLPGLNGENQKEPEQGERGEAGPPSTPNLEGNNRKNPLKGLDGENKPKEDLDG
    KGLSGENDESPKLKDEHPYNHGRRDGYRVGYEDGYGGKKHKGDYPKRFDESSPKEYNDYSQGYNDNYGNG
    YLDGLADRGGKRGYGYSYNPD
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Parts of the three recombinant proteins (FNZ (cell surface-bound fibronectin binding protein), SFS (secreted fibronectin binding protein), and EAG (alpha2-macroglobulin, albumin, and immunoglobulin G [IgG] binding protein)) were used to immunize mice, either subcutaneously or intranasally, prior to nasal challenge with S. equi subsp. equi. The adjuvant used was EtxB, a recombinant form of the B subunit of Escherichia coli heat-labile enterotoxin. It was shown that nasal colonization of S. equi subsp. equi and weight loss due to infection were significantly reduced after vaccination compared with a mock-vaccinated control group. This effect was more pronounced after intranasal vaccination than after subcutaneous vaccination; nearly complete eradication of nasal colonization was obtained after intranasal vaccination (P < 0.001) (Flock et al., 2004).
  • Related Vaccine(s): S. equi Subunit EAG, FNZ and SFS Protein Vaccine
13. sfs
  • Gene Name : sfs
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. zooepidemicus MGCS10565]
  • NCBI Protein GI : ACG61768
  • Other Database IDs : CDD:274741
  • Taxonomy ID : 552526
  • Protein Name : fibronectin-binding protein Sfs-like
  • Protein pI : 8.85
  • Protein Weight : 36230.61
  • Protein Length : 442
  • Protein Note : KxYKxGKxW signal peptide; TIGR03715
  • Protein Sequence : Show Sequence
    >ACG61768.1 fibronectin-binding protein Sfs-like [Streptococcus equi subsp. zooepidemicus MGCS10565]
    MRKTEGRFRTWKSKKQWLFAGAVVTSLLLGAALVFGGLLGSLGGSSHQARPKEQPVSSIGDDDKSHKSSS
    DSMVSRPPKKDNLQPKPSDQPTNHQHQATSPSQPTAKSSGHHGNQPQSLSVNSQGNSSGQASEPQASPNQ
    GPSQPLGLRGGNSSGSGHHHQPQGKPQHLDLGKDNSSPQPQPKPQGNSPKLPEKGLNGENQKEPEQGERG
    EAGPPSTPNLEGNNRKNPLKGLDGENKPKEDLDGKGLSGENDESPKLKDEHPYNHGRRDGYRVGYEDGYG
    GKKHKGDYPKRFDESSPKEYNDYSQGYNDNYGNGYLDGLADRDGKRGYGYSYNPD
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Flock et al., 2004)
14. spaZ
  • Gene Name : spaZ
  • Sequence Strain (Species/Organism) : Streptococcus equi
  • NCBI Protein GI : ACG61542
  • Other Database IDs : CDD:330553
    CDD:307064
  • Taxonomy ID : 552526
  • Gene Strand (Orientation) : ?
  • Protein Name : protective antigen-like protein, fibrinogen- and Ig-binding protein precursor
  • Protein pI : 4.83
  • Protein Weight : 41874.96
  • Protein Length : 541
  • Protein Note : RecF/RecN/SMC N terminal domain; cl25732
  • Protein Sequence : Show Sequence
    >ACG61542.1 protective antigen-like protein, fibrinogen- and Ig-binding protein precursor [Streptococcus equi subsp. zooepidemicus MGCS10565]
    MKSCKSTLRKAAMAAMLAGAITTTGGVKAEENFDFYNHVMQFLEDKSDDESYEKLGKAFEKLPEEQMKAL
    FRIWFSADNSDGISQSVMSIAHKLKEKEQLEFKEVGEMLWNSVTDELDRRIDSEASLKKDKVELQDNLNK
    AIKVSTDLMNQLGAAKADRDKAQAEADRLSQAVDSLGRLTNSAKRELTEKLAEIDQLTQDKAKAASDLET
    ANANITTLQEELAGAKASYDKLSEDKAAADQALAEANVRVSELEQQVAAANNRVVDLEAQLAEKTAELDM
    VKADKAELEAKVKELQSLLEQKDRSNADLQANIERLKQELADKFKDQAQPRAKTPQASNLVAKQKASRPA
    AKPAGQLPSTGEDAANPFFTAAAVAIMTGASLVTVMPKRQKD
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Wei et al., 2012)
15. surface-anchored protein
  • Gene Name : surface-anchored protein
  • Sequence Strain (Species/Organism) : Streptococcus equi
  • NCBI Protein GI : KIS15526
  • Taxonomy ID : 1381082
  • Gene Strand (Orientation) : ?
  • Protein Name : surface-anchored protein
  • Protein pI : 4.68
  • Protein Weight : 105684.48
  • Protein Length : 1089
  • Protein Sequence : Show Sequence
    >KIS15526.1 surface-anchored protein [Streptococcus equi subsp. zooepidemicus Sz4is]
    MEQRKFKKILALVLTVLTMLSSLSFGTNAVNANGTDKYVGWSEYQGSYSAAFYLGKENSTDRYVAYCYNK
    KREAPPRKIDGTVYYQKTGGTPQEYGQSLYHPMTDQQKEETLIKDLRRVLYNGYPHNKGKLSEQFSSVPE
    VQRDGLFREVTQWGVWHYTDGFDPEVYYKKDFELASGSAKQMNELKVKVYKTLIGQGLQEADSSFEMDLF
    TTSQTSQETGNRYQNILVTKDNMPLPKPPDTPYGREEKIYITKVGVINGMIERNALSDAKLEIREGEGLT
    GTALASNITIYPDQFEFSGFEFDLRTYGKRFGDVFTLVETQAPPGYKKSEPIVFMVAGVDELGGDYWIQI
    KQPDGTWKQQELPDKSKVQDGDDLDENSLKQTLIVYDEKQNPEYDITFKKMDETGDYLDGAEFALYKEDD
    PTKAIKTFITGKQDINLKLEAGNYILRERSAPTGYVGVEEDIKFTVNNQGQVTAHGSTPEGVTFNAQSIE
    CKNKKEVAEETVTLKVKKLWDNQEKAEHPETKFGLFKDGVLQIDKVQTLSNGMTEITWENIKKSEYQSYQ
    PFELIEKDGQLEKAGKYITLEDKTYEVFSDLEWKDEGNNVYSIKINNYLTNIITIDKEWQDKDGRKLEDD
    LPSINIHIEMDNGAREDKTFDTDNTSNEYYISATLKIESFFEENVKLQLNNQVEVTVDGKVYLATLTQTS
    ENHYKVVNKEKALGHVVKISKKSGSETGAELKDATLKVFREADDETTAQPIKEWKTKETPEELLLEEGSY
    KLVESEAPQGYRKAETIHFTVNDDGTIAVTGQQLQPGEPIVMVDKAIQTVTIEKKVTGVNGNKQKEFNFK
    LTVNADDDLKNGEVLDGKLYKDGQVLDVNIMVGREYLFNLKDTDKLEVTGLVAESTYTLNEDEYNKDGYT
    TTVMVNDKEKKNADTDATYTVVDGKNNVIFINHNEKIVPTGILFDIMPYLIGMAFVTLSSIAFLLSVKRR
    IVLKNAKK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Wei et al., 2012)
16. szm
  • Gene Name : szm
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. Zooepidemicus
  • NCBI Protein GI : AKL88101
  • Other Database IDs : CDD:273479
    CDD:302998
    CDD:290581
    CDD:295219
    CDD:287215
    CDD:279132
  • Taxonomy ID : 40041
  • Gene Strand (Orientation) : ?
  • Protein Name : Szm
  • Protein pI : 5.64
  • Protein Weight : 56605.01
  • Protein Length : 608
  • Protein Note : Gram-positive signal peptide, YSIRK family; TIGR01168
  • Protein Sequence : Show Sequence
    >AKL88101.1 Szm [Streptococcus equi subsp. zooepidemicus]
    MFLRNKKQKFSIRKLSAGAASVLVATSVLGGAAVEFTSGTIYAEEKIVEADSAKFFDLMVEKKLYDEFSR
    FGYGKKPEEVLASLKQVLKDASPQTLNSLINTLDNGRIDYQTGMGQVFSQAFGSFRADHDPEYLAYSFYS
    LLIQAIKERVVNESELKVQLQEKEEAVEVLTKILDKTVNEKIEIDRKAKEAEGKLKESSLKLTKAYKITK
    DLADQLSNVKQQRDKAFKVSKDLADKLSNAEASRDKAFAVSKDLADKLSSAEASRDKAFAVSKDLADKLA
    AKTAEAEKLMQNVGSLDRLVESAKREMAEKLAEIDQLTADKAKADAELATANDTIASLQTELEKVKTELA
    VSERLIESGKREIAELQKQKDASDKALAESQANVAELEKQKAASDAKVAELEKEVEAAKAEVADLKAQLA
    KKEEELEAVKKEKEALEAKIEELKKAHAEELSKLKEMLENKDHANADLQAEINRLKQELADRIKSLSQGG
    RASQANPGSTTAKAGQLPSTGESANPFFTIAALTVIAGAGMAVVSPKRKEN
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Velineni and Timoney, 2013)
17. SzP
  • Gene Name : SzP
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. Zooepidemicus
  • NCBI Protein GI : AAB92635
  • Taxonomy ID : 40041
  • Protein Name : M-like protein
  • Protein pI : 4.25
  • Protein Weight : 6257.51
  • Protein Length : 132
  • Protein Note : protectively immunogenic surface protein; hypervariable region
  • Protein Sequence : Show Sequence
    >AAB92635.1 M-like protein, partial [Streptococcus equi subsp. zooepidemicus]
    IPELEKKIQEVQEKLNYENRLSPERVGSDDEDDTEARRLIAEQEALKAELQK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Lin et al., 2011)
18. vicK
  • Gene Name : vicK
  • Sequence Strain (Species/Organism) : Streptococcus equi subsp. zooepidemicus MGCS10565
  • NCBI Gene ID : 6760966
  • NCBI Protein GI : 195978500
  • Locus Tag : Sez_1395
  • Genbank Accession : CP001129
  • Protein Accession : YP_002123744
  • Taxonomy ID : 552526
  • Gene Starting Position : 1433586
  • Gene Ending Position : 1434938
  • Gene Strand (Orientation) : -
  • Protein Name : TCS sensor kinase VicK
  • Protein pI : 5.4
  • Protein Weight : 49245.51
  • Protein Length : 450
  • Protein Note : similar to ref|YP_600051.1| Two-component sensor histidine kinase VicK [Streptococcus pyogenes MGAS2096]
  • DNA Sequence : Show Sequence
    >gi|195977156:1433586-1434938 Streptococcus equi subsp. zooepidemicus MGCS10565, complete genome
    ATTAATCAATATCATCCTCCCATTCATCATAGATAGCAGCATCCTTTTCATAAGGTAACACAATGGTAAA
    GGTTGAGCCCTTGCCATAATCACTCTTAGCCCAAATAAAGCCTCGGTGCTGCTTAATAATTTCTTTAGCA
    ATCGCTAGGCCTAGCCCGGTCCCTCCTTGAGCTCTACTCCTAGCCTTATCCACTCGGTAAAAACGGTCAA
    AAATCAAAGGCAGGTCCTTTTTTGGAATACCCAAGCCCTCATCAGAAATCGAAATGATTAGCTGGGTATC
    TGTTGTTTTCATGTTGACGACAATTTTTCCGCCATCGGGTGAATATTTGATCGCATTGTTTAAAATATTT
    TCGATAACCTGCGTCATTTTGTCATCGTCAATTTCTAGCCAAATAGAGGTAATGGGGTAATCTCTGACAA
    TTTCATAGGATTTTCCTGTGCTTGTGTGCTGATTTTTCACCAAATCAAAGCGGTTTAAGATAGAGGTCAT
    AAAGGCTGTAAAGTTGGTCATTTCAACAGACAGCTGTGTCACATGATTATCAATTCTTGAGAGGTTGAGC
    AAATCTGAAATCATGCGCATCATGCGATTGGTTTCATCTAGTGAGACCTTTATAAAGCTTGGAGCGATGT
    CCTCCTTTAGCGCGCCCTCATCTAGAGCCTCTAGGTATGACTTGACAGAGGTCAAAGGTGTCCGCAGCTC
    ATGGCTAACATTTGAAACAAAGAGTCTGCGCTCGCGCTCTTCCTTTTCCTGCTCAGTCGTGTCATGAAGT
    ACAACAACCAGACCTGAAATAAAGCCGCTCTCTCGCCTATTAAGCGCAAAGCGCAGCCGCAGGGAAACAA
    ATTCTCCTGTATCATTACGACGGTTTAAGGTCACAATCGGTGTTTTTGAGACCAGCTCTCGGTAGGTATA
    AGGGGAGTCACTGTCTAATAAATCTGTAATGTTTTTTTCCAAGGCCTGCTCGCGATTTAAATTGAGCTGC
    TTTTGAGCAGTCTCATTAATCATGATAATCTTGCCGGAACGATTGGTCGCAAGGACCCCATCTGTCATAT
    AAGCCAAAATACTAGCAAGACGATTTTTTTCTTGAGCAAGGTTTTCATGAGTCAGACGAAAAACATCTGA
    TAGGTCATTGAGCTGCTCAGACAGCTCCATCAATTCAATATCAGCCTTTTCATTGATCGTATCAGTATAG
    CGACCATTGATCAAATCTCTTATCTTGTGGCTCATCAGACGAATGATCCGAGCATTGCGATAATCACGAA
    TGGCTAAATGAATGAAATAAAAAGCAACAAAAACAAGCAATAAGAGTATGGCTAGCTCAAATGCAGAAAG
    GTTTCCAATGATGTCCTTAGTCA
  • Protein Sequence : Show Sequence
    >gi|195978500|ref|YP_002123744.1| TCS sensor kinase VicK [Streptococcus equi subsp. zooepidemicus MGCS10565]
    MTKDIIGNLSAFELAILLLLVFVAFYFIHLAIRDYRNARIIRLMSHKIRDLINGRYTDTINEKADIELME
    LSEQLNDLSDVFRLTHENLAQEKNRLASILAYMTDGVLATNRSGKIIMINETAQKQLNLNREQALEKNIT
    DLLDSDSPYTYRELVSKTPIVTLNRRNDTGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERR
    LFVSNVSHELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNHVTQLSV
    EMTNFTAFMTSILNRFDLVKNQHTSTGKSYEIVRDYPITSIWLEIDDDKMTQVIENILNNAIKYSPDGGK
    IVVNMKTTDTQLIISISDEGLGIPKKDLPLIFDRFYRVDKARSRAQGGTGLGLAIAKEIIKQHRGFIWAK
    SDYGKGSTFTIVLPYEKDAAIYDEWEDDID
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A vicK mutant is attenuated in mice and induced protection from challenge with wild type S. equi (Liu et al., 2008).
  • Related Vaccine(s): Streptococcus equi vicK mutant vaccine
1. IgA
2. IgG
  • Gene Name : IgG
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16059
  • Genbank Accession : AF010213
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 881411
  • Gene Ending Position : 914690
  • Gene Strand (Orientation) : +
  • Protein Name : immunoglobulin heavy chain (V7183 family)
  • Protein Note : Also known as IgG; IgH; VI24H; VH7183; B9-scFv; IgVH1(VSG)
  • DNA Sequence : Show Sequence
    >gi|94393741:881411-914690 Mus musculus strain 129/SvJ chromosome 12 unlocalized genomic contig, MGSCv37 alternate locus group 129/SvJ
    CATGGACTTTGGGCTCAGCTGTGTTTTCCTTGTCCTCATTTTAAGAGGTAATTTGTAGAAATAAGATCCT
    GCCAGTATTGTGTACAGGAGAAATAGAAAAATTTTTCTTTCCTCTATTTTGTTTTGTTTTGTTAGTGACA
    GTTTACAAATAAGCATTCTCTGTTGTGAGGTGCCCAGTGTGAGGTGAAGATGGTGGAGTCTGTGGGAGGC
    TTAGTGCAGCCTGTAGGGTCATTGAAACCCTCCTGTGCAGCCTCTGGATTCATTCTCACTGACTACTGAA
    TGACCTGGATCCTTCAGGCTTCAAAGAAAAGGATGGAGAGGGTGACAATAAAATTTTTCCCACTCACACC
    CACTTGCTGGCCCTGGCGTTCCCCTGTAATGAGGCACATAAAGTTTGCAAAACCAAGGGGCCTCTCTTCT
    CAATGATGGCTGACTAGGACATCTTCTGATACATATGCAGCTAGAGACATGAGCTCCCGGGATACTGGTT
    ACTTCATATTGTTGTTCCCCCACAACAGTTGCAGACCCTTTTATTTCTGTGGGTACTTTCACTAGCTCCT
    CCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTAGTTGCTAGGCCCCGG
    CATAGCCTCACAAGAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTTGCTGGTGTATGCAATGGTGTC
    AGAGTTTGGAGTCTGATTATGGGATGGATCCCCAGGTATGGCAAACTCTAGATGGTCCATCCTTTCCCAA
    CAATAATTTTTAATGGTGGAGATAGCACCTACTATCCAGACACAATGAAGGGCCAATTCACTATCTCTAG
    AGATGATGCCAAAAACACACTTTACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTATACA
    TATATTCCTAAGCTTGAGTAAGTGGACTTAAACTGATTCCATCACAACTTGCATGAGATGGATATTCCCC
    AGTGTTAAGACCTGTCACCATCACTGTCAATCAGAAGACAAAGTTTATGCACAACAAAACAAAAAAACCA
    AAAGCAGAGGCCTCCAATTACAAGTAATAGACCCAGACCCACAGTCTCTGAAAACTGACTGTACAGTTGG
    ATCCAGTCTTTTACTTCTTTTCCCTGGATTTTATATTACTGAGGAAATGAGGAAAGCTCTACAATATCTG
    TTCTCCATAGTGCTCAACACCTCCAAGCACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTTCTT
    GTCTTGTAGACTACTTCAACCTATTTTGTACTCCAGTTAATGAAACTCAAGTCTAGCAGCCTGTCACTGT
    TTATTCTAAAGTATTATGAACAGGTGACCTCCCATCCTTCCCCAACGCAATAATCATATTTAGGAATTTG
    AGGTTTTATGAGATATGATCTCAGGGTAGAGAGAGAAAGCAAACTACATAGAAATATAGACTGACATAAA
    TCAAGACTTGCATAAGCTAGTCCCCAAGTTCCATGTCCCTAAGTGGCAAGGACTATCTTCTGAGCCTAAT
    GAGATGAGATCCAAATCAAACCTCCTGGGTCTTTTTAGATAAACATGTGAGATCAATAGACTAAATGCTT
    TGGCTGGGCTTCCTTGCAATCCAATTCCCAAACAAAAATGGATCTGGCTCCACAGACACCACAAGAATAG
    TCTTAAATAGTTCTTTAAGTAGAATGTCTGATCACTACGAGCCCAATTCCATCCTAAATACTCTTCTGGA
    TTATACATAAATAAAAATTGAACATAGGGCATGGGGCACTGATCTCCCTGTGCTACATGAATGGGGGCTC
    ATTTACTAAATGTTCCCATTTTTCTTTCTAGCGCTGCACAGTGCAAATCCTACAACTTCCTGTTTATCTA
    CAATGTGAACTCCAAACAGTACAAGAAAAAACGTTCCTTATGTTCCTCTCCAGGTTCTCCAACAAGCACA
    GAGCAACCTTCTGTCACCAGGACGGAAAACTGGAAACCTTGCTCACTGCTTCCTTTTATTCCCTCGGGAA
    CCTCCCCCAATGCAAAGCAGCCCTCAGGCAGAGGATAAAAGCTCACACAAAGACGAGAAGCCCCATCCTC
    TTCTCATAGAGCATCCATCAGAGCATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACTTTCCCAAGCT
    GTAAGTGTTTCAGGGTTTCAGAAGAGGGACTAAGACATGTCAGCTAGAAGATGTGTGACTAATGGTGATG
    TTGCTTGTCCCCAGGTGTCCTGTCCCAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCA
    CAGAGCCTGTCCATCACATGCACCGTCTCAGGGTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCC
    AGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGC
    TCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTC
    CAAACTGATGACACAGCCATGTACTACTGTGCCAGAAACACAGTGTGGGAAGTCCAATGTGAGCCTGCAC
    AAATACTTCTCTGCAGGGATGATCACAACCAGCAGGGGGCGCTGATGACCCAAAGGGACTTCCCAGGATC
    TCTTCTGGAATCTAGGGAGTTCTGGCCTGTGTCTATCAGCATGTGTTTCAATGTTAGAGTTGTGAGTTTT
    CCTTCCAGCAACAGAGATATTTTAGAGCCCACTTTTCATGGTCATTCTACTAAATTTGTTCACATAGTGG
    AAAGATTTGTTAAATGATTCTATAGTCTAATAGGGTCAAACAAAAACAAATAATTGAGTTCATATCTACC
    AAAAAAAAAAAAATTCCCTCAAAGTGGACAACTGTGTAGGTGAGGAAAACCAGGGGGATTTGGGAATAGA
    TTATTTCTCTCCCGACTGTGGTGTTAGTCACCTTTCAGCCATTTACTGTATTTAATTCATGTTTAAAGTT
    AATTGTATTCATCTTCCCTAACATGAAGTTTTCAAACACATGTCCACAATTTAATGATGACACAGTTATC
    ATTGTGAATGATAACTCATAAGATCTCTCTCCATATATGAAACACACGATATTCTGTTATTAGCTATAGC
    CAACAAAGTTACATATCATTATTATTACTGAAATAATTCTTCCCATCTAACTGAATAGTTTTCTCACTCG
    ACACTGCCTATGCTGCGATCAGCCTGCTCTAATTTTACCTTTGCTCAGAGCACTGCTTTCTGTTTCATTT
    GATAAGATGGTGCCTGGTTGTCACTGAAAATGTGTCCTCCAGTTTCACAGTGATTAATGTGATTTTCAGT
    ACATTGAGAACAGAGCCACAGCAAAAATGAGATGGAATGTCATCATTTTTACGCCAGGTGCTGCAGAAAT
    AGCTAGGTGGTTAAGAGAGAATATTGAACTTTGAGGGGTTCAGATTAAATTCCTGATACCCACAATGTGC
    ACCTCATAACCACATGTACATATAGAAAACTCAGTATAAATGTGGCCTCCGTGAGCACCACACTACTCCC
    ACAAACACATATACACATAATTAAAAGTAAAGATTTTAGAAAAATGGCTAATCTAATGATAGGAAGTAGT
    CAAAAAGAGAGTTCTTTTGTCATGTACATATGTGTAGCAGACTTAAATGTTAAACATTCAAGAATACATT
    CCTCGATCCACATTAAAATTTTGCAAAGAGTAGCACAGACGGTGGCAATTGCTAAACTTATATCTAGAAA
    CACAATTATGTGTGGTGATATTTAATTACACATTTATACCAGGACATATGACAATATGGAAACCAAACAT
    GTTGTATCCACATGCTCTAAGGAAAACTAAATGGAGTGTGATAAAACCAAAGAAAATGTGAATATGAAAT
    ATTTTTCCAACTCTGCATCTTAAAACGGTTTCTTTCATGTGTCATATCTGCTATGAGGACTTTCTTCTGC
    CCATGTCCAACTCCAGAGCATGCCACAGCAGGAAGACCTACAGGTATTACTTCTCTGCACCCAGGAAAAC
    CACCTCTGTCCTGACCCTGCAGCTCTCAGAAGAGCCCAGACCTTCATTCTCAGGCCCTCATCCAGTAATC
    AGCACTGAATACAGAGCACTCACCATGGACTTTGGGCTCAGCTTGGTTTTCCTTGTCCTTATTTTAAAAG
    GTAATTCATAGAGATAAGATTCTATCTGTTTTGTGTACATGAGAAACAGAAAAATTGTATTGTTTCTCTA
    TTTTGTTTTGTTTTGTTAGTGACAGTTTCTGACTCAAGATTCTCTGTTTGAAGGTACCCAGTGTGAGGTG
    AAGCTGGTGAAGTCTAAGGGGAGGCATAGTGCAGCCTAGAAGGTCCATGATACTCTACTGTGCAGCCTCG
    GATTCACTGTAAGTGACGACTGGTTTGTCCGTGTTTGCCAGGCTCCAAAGAAGGGGCTGCAGTGGGGGAT
    GGGAATAATTTTTCATGGTTGTGGTAGCCCCTCTTATGCAGACACCTTGAAGAAGTGGGTTGGACGTAAC
    ATATTCAGAATCAATATTTAAAGATTCTAATCCTTGAAGATATCACTTTTGACCAAGTATATATGAACCA
    TGTTACTGAGGTTTATGGAGGTTTGAGTATGTTAGGTCCATGGATAGGGAAAATATTAGGGGATTTTAGG
    AGTAACTGAGGCTTGTTGTAGGAAGGACATCACTGTAGGGGTAGGCTCCGTGTTCCTATCTTCAAGCTCT
    ACCCAGTGCAGAATAGAGCCCTCTTCTGCCGGCAAGTGGAAAGAGTTTCTCTTCCTGGCTGCCTTCAATC
    CAAGTTGTAGAATATCAAATCTCCTAACACTATGACTGCGAGCATGCTGACATGCGTCCCACCATAAAAA
    TAGACTGAACCTCTGAAGCTTTAAGCCAGCCTCTAGTAACTGTATTCTTTAATAAGACTGAACTTGGCTA
    TGGTGTCTTTTCACAGTAAAATGGAAACATAGACAAGGTTCTAACTCTCTGTACTAGAGGACCATCTCTA
    TGTCCTTGGTGTTGTATGTAAATTATTGAACAAATACTATAGACAAGTTGTATGGCCAAAAGTCCATCCC
    CTTCAACAATCTACTATCTGGAACCATATAGATTAGATTCCTTTCCTGCACTCATTTCCCTTACTTGCTG
    AGACATTTTGAAGACATGCTCAGAATCCCTGAACTTCCTGCTGAAAAAAAAATACCCCTCCAATTTGAAG
    ACAGTTCTCTTCCAAAATTCATCATAAATACTGATCCACATGTCCAGGCATATCATGTAGTTTTAAAAAA
    CAAACCAATCAATGCTAAAGTGGGTAAGTGCCTACATCATTGGTTTATGTCTGTTCATAACCTGGTATAT
    AGCTAGAGACCAGGACTGCATGTTTCTCCTAGGCCACACCTCTCCCACAAATGCTGGCTCTGCCTCTCCA
    GAATTCCAAGATCATGGATCTCTGGGTCCCCAAGGAACAATAAGTGTGCACTCTTGCCAGCCTGTACACT
    ACTGCACTAGCTTCTCCCCCTGGAGCTTGGTCATTGCTCAATCTTCCCTTTCCAAAGCCTGGCCAAATTC
    CTCCTACCTTTTGTTCTTTCAGTCAGGCCACCTAAGTCATCTTGAATGAAAAACAACACAAAATCTGAAC
    TTAAAATCAACAAATAATACAAGTGCTGCGCACAAAATCAATCATTCTAAACTCATCAACTATTTTATGA
    TGGAAATCTTCCAACATACAAGCTACCACCAGGTCTAAGAATTACTCATCTACATGTTGCTTCCTCTCAC
    TCACAGCCTAACCATAAAAGGGCTGTTTCTTCCTCCCATGTCCCCTCTTTTGCCTTCAGAAGCAGAAGCT
    CCACCTCCTCCTCTTTGCCCAGCAATTGGCTTCTTGTCGTCTTTATTATCATATTAATTACTTAGGGGAA
    AATCCCGTGTAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGG
    AAGGCTCACCAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAG
    CCATAGGGGCTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACG
    CCTTTCATCTGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAA
    TATAAGGACATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTG
    AGTAACTGCTAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGA
    CTCTAAGTCATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTG
    ACTAATACAGAAGTTGGTACCAGGAGTGGTTCTAGAGTAACAGAAGTACAAGGATGAATCTTTTAAAATA
    CTGGAATTGGCTTGTTGATCCACCAGCACTTTCAACTATTGAAACCTCTCCAGATTCTCTCCCTCCTGGG
    AGCTCAGAGAATTTTGAAGACCCATGGTTGAAACTATATTCCGAACTTAAAGAAGCAAATGCCCTTGATT
    TTCTTAATGAATTAGGTGATTCAGTGCACAAAGCTTTCTACAAGATGGGGAAAAAATTGGAAAATGATTT
    TACTGGCTGGCTGCTCTTAGTATCTGTGGAAAAAATGATGAATGAAAGGAAGGAGTTGTGTGATAAAATC
    GAAAGGCTCCAGACACAAGTAAACGATCTAAAAGTTGCTAAGTGTGTCCTTGAGGAGAATCTTCTCTCTT
    GTAGCAATAGAGCTCAAGTTGCAGAAAATCAAACAGAAACTCTCATTGTAAGGTTGGCTGAACTACAGCG
    AAAATTCAAGTCTCAGCCTCAGAGTGTGTCAACAGTTAAAGTAAGGGCTCTAATTGGCAAAGAATGGGAT
    CCTACAACATGGGACGGGGATGTGTGGGAAGACCATGTTGAAGCTGAGAATTTTGAATCTTCAGATTCTC
    AAGGGTTTGCCCCACCTGAGGAAGTAGTACCCTCAGCCCCACCCCTTGAAATAATGCCTTCCCCACATGA
    GGAAATTAATTTTGCAGAGTCTGATAAACCAGCAATGATTTTCACTACTGATGTTTCTCAAGGCCCACCA
    ATAGTTTCTTCTAGACCTGTAACCAGACTCAAAGCAAAACAGGCTCCTAGAGGGGAGGTAGAAAGTGTAG
    TCCATGAGGAAATTCGCTACACTACTAAGGAGCTTAATGAGTTTGCTAATTCATTCAAGCAGAAACCTGG
    TGAATATGTGTGGGAATGGATTTTAAGGGTGTGGGATAAGGGTGGAAGGAACATAAGACTAGAGCAGGCT
    GAGTTTATTGACATGGGTCCTCTGAGTAGAGATTCTAGGTTTAATACGGAAGCTCGCATAATTAAAAAAG
    GTGTCAAAAGTTTGTTTGAATGGTTGGCTGAGGTGTTTATCAAAAGATGGCCTACTGGAAATGACTTGGA
    GATGCCTGATATTCCGTGGCTTAGTGTTGATGAAGGGATTTTAAGACTTAGGGAAATTGCAATGCTAGAG
    TGGATATATTGTGTAAAGCATAATTGTCCACAATGGGAAGGTCCAGAAGATATGCCTTTCACTAGCTCTA
    TAAGACGCAAATTGGTGAGAGGGGCACCAGCACATTTGAAGGGTTTTGTTCTTTCCCTTTTCCTTGTACC
    AGATCTTAGCATTGGAGATGCTTCTGCTCAATTAGATGAATTAAATTCACTGGGTTTAGTTGGATTCCGA
    GGTAACAAGGGCCAGGTGGCAGCATTGAATCGCCGGAGACAAGGTGATTCTAGTTATTATAATGGACAGC
    GTAGACAAAAGAATGTTTATAATAACATACCCAGCAATGGTCAGCACAGGAGAGGTGAAATTTATAATGG
    CATGACTCGGTTGGACCTTTGGTACTGGCTAACCAATCATGGTGTTTCCAGGAATGAAATACATAGGAAG
    CCTACTGCATATTTGTTTGATCTGTATAAGCAGAAAAATTCTCAAACAAATGAAAGAAAGGCTACATTAG
    ATCATGGTAAACAGCAATCTCGGCCAGTGAATCAATTTCCAGACTTGAGACAGTTTGCAGATCCAGAACC
    CCTTGAATGAAGGGGTGGCCAGGTTCCGCTGAGGAAGGATCTTGATAAGACACCCAAAGGTTTTGCTGTT
    ACCCTTTCTCCAGTTCTTCCCCAGAGGGACCTAAGGCCTTTTACAAGGGTAACTGTACACTGGGGAAAAG
    GAAATAATCAGACTTTTCAGGGTCTGCTGGATACTGGTTCTGAGTTGACACTGATTCCAGGGGATCCCAA
    GAAACATTGTGGCCCTCCAGTTAAAGTAGAGGCTTATGGAGGGCAGGTGATTAATGGAGTTTTGACTGAT
    GTCCGACTCACAATAGGTCCAGTAGGTCCCCAGACACATCCTGTGGTGATTTCCCCAGTTCCAGAATGTA
    TAATTGGGATAGATATACTCAGAAATTGGCAGAATTCTCATATTGGTTCCCTGAACTGTAGAGTGAGGGC
    TATTATGGTTGGAAAGGCCAAATGGAAACCTTTAGAGTTGCCTCTGCCAAAGAAAATAGTGAATCAAAAA
    CAGTATCGTATTCCTGGAGGCATTGCAGAAATTACTGCCACTATCAAGGACTTGAAAGATGCAGGGGTGG
    TGGTTCCCACCACATCTCCGTTTAACTCTCCTATCTGGCCAGTGCAGAAAACAGATGGATCATGGAGAAT
    GACAGTTGATTATCGAAAACTAAATCAGGTAGTAACTCCAATTGCAGCTGCTGTACCAGATGTAGTTTCA
    TTACTTGAGCAAATTAACACATCTCCTGGCACCTGGTATGCGGCTATTGATCTGGCAAATGCCTTCTTCT
    CAGTACCTGTCCATAAGGACCACCAGAAGCAATTTGCTTTCAGTTGGCAAGGCCAACAGTATACTTTCAC
    AGTTTTGCCTCAAGGATATATTAACTCTCCTGCCCTGTGTCATAATTTAGTTAGAAGGGATCTTGATCGT
    TTGGATCTTCCACAAAATATCACATTGGTGCACTATATTGATGACATTATGCTGATTGGACCAAGTGAGC
    AGGAAGTAGCAACCACTTTGGACTCATTGGTAACACATATGCGTATCAGAGGATGGGAAATAAATCCAAC
    CAAAATTCAAGGACCATCTACCTCAGTGAAATTCTTAGGAGTCCAGTGGTGTGGAGCATGCAGAGATATT
    CCTTCTAAGGTGAAAGATAAGTTATTGCACCTGGCCCCTCCTACAACCAAGAAAGAAGCACAACGTTTAG
    TGGGTCTATTTGGATTCTGGAGACAACACATCCCTCACTTGGGTGTGTTACTTAGGCCTATTTACCAAGT
    GACTCGGAAAGCTGCTAGCTTTGTGTGGGGCCTGGAACAGGAGAAGGCCCTTCAACAGGTCCAGGCTGCT
    GTGCAGGCTGCACTACCACTTGGACCATATGACCCAGCAGACCCGATGGTACTTGAGGTGTCTGTGGCTG
    ATAGAGATGCTGTTTGGAGCCTCTGGCAGGCCCCTGTAGGTGAATCACAGAAAAGACCTTTGGGATTTTG
    GAGCAAAGCTCTACCATCATCTGCAGACAACTATTCTCCCTTTGAAAAACAGCTCTTGGCCTGCTATTGG
    GCCTTAGTGGAAACTGAACGTTTGACAATAGGACACCAAGTTACTATGCGACCTGAACTACCCATCATGA
    GCTGGGTACTATCAGACCCTGCAAGTCATAAAGTGGGACGCGCACAGCAGCAGTCTGTTATCAAATGGAA
    GTGGTATATACGTGATCGGGCCAGAGCAGGTCCTGAAGGCACAAGCAAGTTACATGAAGAAGTTGCTCAA
    ATGCCTATGGTTTCTACTCCTGTTACACTGCCATCTGCTGCCAAACATGTGCCTATAGCCTCATGGGGTG
    TTCCCTATGATCGACTGACCGAAGAGGAAAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTTATGC
    AGGCACCACCCAGAAGTGGACAGACAGCTGCAGCATTACAACCCCTTTCTGGGACAACCCTGAAAGACAC
    AGGTGAAGGGAAATCTTCACAGTGGGCAGAACTTCGGGCAGTACACATGGTATTACAGTTTGTTTGCAAG
    AAGAAATGGCCAGATGTACGATTATTCACTGACTCATGGGCTGTAGCCAATGGATTGGCTGGATGGTCAG
    GGACTTGGAAAGATCACAATTGGAAAATTGGTGAGAAAGACATCTGGGGAAGAAGTATGTGGATAGATCT
    CTCCAAATGGGCAAAGGATGTGAAGATATTTGTGTCCCATGTAAATGCTCACCAAAAGGTGACTTCAGCT
    GAGGAGGAGTTCAATAATCAAGTGGATAAGATGACCCGTTCTGTGGACAGTCAGCCTCTCTCCCCAGCCA
    TCCCTGTCATTGCTCAATGGGCACATGAACAAAGTGGCCATGGTGGTCGAGATGGAGGTTATGCTTGGGC
    TCAGCAACACGGGCTTCCACTCACCAAGGCTGACCTGGCTACAGCTGCTGCTGATTGCCAGATCTGCCAA
    CAGCAGAAACCAACACTGAGTCCCAGATATGGCACCATTCCTCGAGGTGACCAGCCAGCAACCTGGTGGC
    AGGTTGACTACATTGGACCACTTCCTTCGTGGAAAGGACAGCGTTTTGTTCTTACTGGAGTAGATACTTA
    TTCTGGTTATGGATTTGCCTTTCCTGTACGTAATGCCTCTGCTAAAACCACCATTAACGGACTGACAGAA
    TGCCTTATCTATCGTCATGGTATTCCACACAGTATTGCTTCTGACCAAGGAACTCATTTCACAGCCAGAG
    AAGTACGACAGTGGGCCCACGATCATGGAATTCACTGGTCTTACCACATTCCCCATCATCCTGAAGCAGC
    TGGTCTGATAGAAAGATGGAATGGCCTTCTGAAGACGCAGTTACAGCGCCAATTAGGTGGTAACAGCTTG
    GAAGGCTGGGGTAGAGTTCTTCAGAAGGCAGTATATGCTTTGAATCAGCGCTCGATATATAGTACAGTTT
    CACCCATAGCCAGGATTCATGGGTCCAGGAATCAAGGGGTGGAAAAAGGAATAGTTCCACTTACTATCAC
    TCCTAGTGACCCTCTAGGAAAATTTTTGCTTCCTGTCCCCATAACTCTAGGTTCTGCTGGCTTAGAAGTT
    TTGGCTCCAGAGAGGGGAGTGCTCCTACCAGGAGCTACAACAAACATTCCATTGAACTGGAAGCTCAGAC
    TTCCCCCTGGTCATTTTGGGCTTCTAATGCCCTTAAACCAACAGGCTAAAAAAGGAGTAACAGTGTTAGG
    AGGGGTGATAGATCCAGATTACCATGGGGAAATTGGATTACCTCTTCACAATGGTGGTAAGCAAGATTAT
    GTCTGGAGTGTAGGAGATCCCTTAGGGCGTCTCTTAGTACTACCATGTCCTGTGATTAAAGTCAATGGGA
    AACTACAACAGCCTAATCCAAGCAGGATGACAAAGGACACAGACCCATCAGGAATGAAGGTATGGGTCAA
    TCCTCCAGGAAAAGAGCCAAGACCTGCTGAGGTGCTGACTGAAGGAGAAGGAAATATAGAATGGGTAGTA
    GAGGAAGGTAGTTATAAATACCAATTAAGGCCACGTAACCAGTTGCAGAAACGAGGATTATAAAGTAATA
    TGAATGCCCATTGTAAATTTACTAATGCGTTTGCGATTGTACGAGGGATAGTTATATCATGTTAGGCGTA
    TTTACAAACTTGTTATTGTTTTATGTGAACATGAGATATTATTTGTGTCAAGTTGACAAGGGGTGGATTG
    TAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGGAAGGCTCAC
    CAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAGCCATAGTGG
    CTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACGCCTTTCATC
    TGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAATATAAGGAC
    ATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTGAGTAACTAC
    TAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGACTCTAAGTC
    ATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTGACTAATACA
    TCCCTACATACAGGAAACTTGATGTATAAGTAAAGAAAATAGTAAATCTAAAAATTTCCTTATCAAATAC
    ATGCAAGAAATCCAAAATACCATGAAAAGACCTGATCTAAAAATAGTAGGAACTGAAGGTGAAGTGTCCC
    AGCTCTGAAAACTAGAAAATGTTTTCAATATTTTTCCTAACAGTGAAGACATGGATCTAATGAGGCCACC
    TCTTATAGCCATGCAAGACTGACAGTGGAGGGATAAGGACACCAATCCACCCACAAAACTTTTGACCCTA
    AATCAGTTTTATCTAAAAGCAATGCAGGGGCACAAATGGAGCAGAGACTGGAGGAATGGTCAAACAATAA
    CCTGTCATATCTGAGACCCACCTAATTGGCATACACCAGTCACTGACATTATTAATAATGTTCCATTATG
    CTTACAGGCATTTCTCTAGCATTACTATTCTTGGAGAGACTTCACACAGCATCTTATTGAAACAGATGCA
    GACACCCAGAACCAAACATTGGATGGAGATTGGGATCCTTAAAGAAGAGTTGGGAGGATAATTGAGAGAC
    CTCAAAGGAATAGCAACCCCATAGAAAGACACGAACAGGGTCAGTAAACCTGGACCCATGGGGTATCTCA
    GAGACTGATCACCAACCAAAAATCACAGAGGGCCTGGATTGATTCCCCTGGCACACATGTAGCAGAGGTC
    TGCCTTGTCCTTCAGTAGGTGAAGATGAGCCTAAGGCTTCAGAGACTTGATATATCAGCATACAGCAACA
    CCCAAGTGTGTCCCACTATCTCAGAGGTGAAGGGGTGGAGATGGGCAGAAGGGTCCTGATAGGGGACAAC
    TGCAAAGAGGCAATATTTGGGACAGAAAGAAAGGAAGAGAGAGGAAGATAAAGAGAGAGATGAAGGAAGG
    AAACAAACAAACCAAGATGAAGAGAGAGAAAAGGGGGAAAATAGCCTTGAATGGTTCAGGGATTCAAAGG
    GACATACCTAAGCATGGTAAAACAATGCACATAATGGCAGCAAGTAGCTAACACTGAATTAAATAGAAAG
    ACACATAAAGCAGTTCTACTAAAGTAAGAGACAGAGTTGCCCAATCTCACCCTATTTATTCAATAATAAT
    GTACTTGAAGTTCTAGCTAGGGCTGTAAGACAACTCAAGGAGATCGGAGGATACAAATTAGAAAGAAAAA
    TTCAAAGAATTGTAATTGGTAGATGATAGGATAATATACATAAATGACCTCAAAATTCTTCCAGAGAACT
    CTGAAAGCTGATAAATACCTTCAGCAAAGTGGCTAGAAACAAAATTACTTCATAGAATTCAGGAGCTATC
    CTTTATAGAAAAGTTAAAGTGGCTGAGAAAGAAATTAGGAAAATCACATTCTTTGCAATAACCAAAAATA
    ATATGAATTAGCTTGGTGTGACTCTAATCTTGCAAGTGAAAGATGTGTGTGACAAGAGCTTCAGGACCCT
    GAAGAAAGAAATCAAAGAACTCAAAGATGCAAAGTACTCTAATGCTCATGGATAGGCAGAATTAACATAA
    TGAAAATGCCAAATTTAACAATTCAATCTACAGATTCAGTGGAATTCCCATTAAATATCCAACCCAATTT
    ATTACATTCTTGAAAAAGCAAATCTCAACTTCATATAGAAAAACAAGAAATTGAGGGTAGCTAACAAAAT
    CCTGAACAATGAAAACACTTCAGGAGAATTCACCATCCCCTACCTCAAGCTGTATTTTAGAGCAATAGTT
    ATTAAAACTGCATGGTATTTGTATAGAAACAGATATGATGATCAATGTAATTGAATTGAATACACTGACA
    TAAAACCACACTGTTATGGACACTTGATTTTTGACAAAGAACCCAATAATCATAATAAAAATCATAATAA
    GAATGCATCCCCCAACAAATGGTCCTGATCTAAATGCAAATAGATTCATATCTATCATCCTGCACGAAAT
    TCAAATCAAAGTGAATTGCAGACTTCGACCTAATACTGGATTAACTAAATCTAAATGAACAAAAAGTAGA
    GAATAGTTTTGAACTCATTGATGCAGGATTCAATTTCCTGAACAGAACCACAATGGCTCAGGCTCTAGGA
    TCATAAATTGGTAGATAGGATCTCATGAAACTGAAAAACTTCTGTAAGGCAAAGGCAATAAAACAAAATG
    GCAACCTACAGATTGGAAAAAAAATAATCTTCAGTATCCCTAAATCCCATTGAAGGCCAATATGCAAAGT
    ATATAAAGAACTCAAGATGTTATCCTCCACAAAACCAAATAAATCAATTAAAAATGAACTACAGAGCTAA
    CAAGAGAATTCTCAACAGAGGAATCACCAATGACTGAGAATCACTTAAAGAAACATTCAATATTTGTAGT
    CACCAGAGAATTGCAAATCAAAACTACCCTGAGATTCTACTTTATACCAATCATAATGGTCAAGATAAAA
    ATTCAAATGACAGCGCATGTTGGTGAGGATGTGTATACTTTTGCATTGCTGGTGAGGAAAACAATCTGGC
    AGCTCCTCAGAAAATTGTAAATAATTCTACCTGAAGATCCAGCCATACCACTCCTGTGCATATACATAAA
    ATGTGCTCCACCATACCACTAGGAGATATGTGCCACTATGCCCATAGCAGCCTTATTTGTTAATAGCCAA
    AAGATGGATACAACCCTGATGTGCCTCAAACAAAGATTGTATATAGAAAATATGGGTTCCCTTACACAAT
    GGTATTCTACTCAGCAATTAAAACGTGAGAACATCATAAAGTTTTCAGGCAAACTGATCAAATGAAAAAG
    TATAATCTTGAGTGAGGTAACAGAACCAAAATGACCTGCATGGGATGTACTCACTGGTAAGTGAATATTA
    GACTAAAAGTATAGAATATCAATGATGAAAGCCACAGACCATAAAGAGTTTAATAAGATGGAAAGCTCAA
    GTACGGATTCTTCAATCCAATATAGAAAGAGGGACAAAATAATCAGGGGAGGCAGAGGGAGAGAGAGACA
    TTTGGTGGAAATGGGAGAAGAAGGGAGAAAGGCAGAAAGGATCAGGCGTTGGGGAACACTGGAAAGCAGC
    CCAGGGGTCCTGGTAAATGGATTAAATATTCAGCTGAATGGAGGTGGGAGGCAGGGGGAAGCTCTGCAAA
    GTCCAAGAGACCTGGGATGTGAGAGGCTCCCCAGATGATAATCTTAGCCTTCATGCCTAACAGTTGTAGA
    TAAACCCTGAAGAGATCACTTCCAATAGATGCAGAGGGCCCTAAGTGGTTGGATGGAGTCCCCCAACTTA
    CCTCAAAATTGTCAATACTCATAGAAATTAGGACAATGAAAATCAAAACACCCTGAGATTGTATCTTATG
    TCTGTCACAATGGTTAAGACCAAAACCTCAAGTGATGGCTTGTGCTGGCAAAGATTCAGAATAGTAAAAG
    TCTCCCCATGGCTTGTGGGAATGCAAACTCTTACAACCTCTTTGGAAGTATATTTGATTGTTACCTGGTA
    TAAAATGGCAAAAAAAAAAAATGGTTGAAGGGGGACTAAAAAAGGAAGAAAGGGGAGAACTATGGGATGC
    GAAACAAGAAAGTTTGTTGCAAAAGAAATATGTTTCCACTGCAAACCCTGAGTCTCAGACAGAAGGGGAC
    CTGGAATTCTTCAGATACAAAGAATCTCTAAACCCTGAGGACATTCTATCACAAATAAGTAAAATTCAGA
    AAATTCTGAGTGCTCCCATCACGGAGATGAATCTGCTATGAACAGCTCATAGGTGTGACCCTCTACAAAA
    GCCATATTATTGAAAAGCCACATTGTGCCCAGACTTTGGAAAGACTGAGCTCATATCCTGAAATACAGTT
    ATGTGTGGTTCTATTTAATTACACATTTACACTAAGAAAACATGGCAGTATGGGAATGAAGCTTGTTCTG
    TACACATTAACAGAGGGAAACTAAACAAAGTATGGTGAATCCCTAACCAAAAGTAAAAAAAAAAAAAGAA
    AGAAAAGAAAATAAAAGTGAAACTACAATATGTTTCAAATGCTGTAACTGAAATCTGGTTTTTTGATGCT
    TATATCTGGTATCATCAGTGACTTCAGATTTAGTCCAACTCCAGAGCATGGTATAGCAGAAAGACATGCA
    AATAAGCCTTCTCTCTGCCCATGAAAAACACCTCGGCCCTGACCCTGCAGCTCTGACAGAGGAGGCCAGT
    CCTGGATTCGATTCCCAGTTCCTCACATTCAGTGATCAGCACTGAACACGGACCCCTCACCATGAACTTG
    GGGCTCAGCTTGATTTTCCTTGTCCTTGTTTTAAAAGGTAATTTATTGAGAAGAGATGACATCTGTTGTA
    TGCTCATGAGACAGAAAAATTGTTTGTTTTGTTAGTGACAGTTTTCCAACCAGTATTCTCTGTTTGCAGG
    TGTCCAGTGTGAAGTGAAGCTGGTGGAGTCTGGGGGAGGCTTAGTGCAGCCTGGAGGGTCCCTGAAACTC
    TCCTGTGCAACCTCTGGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCAGAGAAGA
    GGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTATTATCCAGACACTGTAAAGGGCCG
    ATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCCGTCTGAAGTCTGAGGAC
    ACAGCCATGTATTACTGTGCAAGACACACAATGAGGAAATGTTACTGTGAGCTCAAACTAAAACCTCCTG
    CAGAGCACCCAGGACCAGCAGGGGGCGCAGAGAGCACATGGAGTTCTGATTCACAGAAGAGTTACAGCCT
    GTACAATTAGACCCAATCTTCAACAAACCGTCAAAATATTCGATCCAAAATTGTTCCTGTCTAAAAGTAA
    TTCAAGGACAAAATGGACCAGAGACTGAAGAAATGGCTGACCTGTGACCCTCCCAACTTTGGATCTATCT
    CATAGGCAGGTACCAAACCTTGACATTTGTCACTGACACTGTATTGTGCTTGCAGACAGGAGCATAGCAT
    GGCTGACCTCTAAGAGGCTCTGCAAGCACCTGAATGAGACAGATGTAAATAGAGTGTACAACTGGATTAA
    GTTTGAAGACTGCAATGGAAGAGTTAGAGGAAGGAATGAAAAATCTGAAGGGGATCACAACCCCAGAAGA
    AGACCAACAGTGCCAATTAAACTGGATGCCTGGGAACTCCCAGAGACTAAGCCACAAACCAAGAATCATG
    CAGGCTGGTCTGAGAATCCTGGCACCCAAGTCTCAAGGAACTGCCTTTTCTTGGCTCAGATGGGAGAAGA
    TGAGCCTAAAGCTTTAGACTTGACGCCCCAGGGATGGGCAATACAAAGTTGAGTTTCTTGGGAAAGTGAA
    GAGGATGGTGGGAGGAGGAATAAATCTGGGAAGTGGGACTGGTTGGGGACAACATATTGGTCCTAAATTT
    ATGAAATAATTATCTAATTGATAAAAAGAGTCCTTGGGGTAATGGAGGGCTAGACTCCTCTGTGCCTAGT
    TTGTAACTCTACAGGGATCCTCTTTAAAAGAATAGGGTGCACATAGAGTATATGTGTGTGACACTAATAC
    AGGGGTAAGTGTTTCTGTAAGAACTTTATGAATAAACTTTATTAAAACATCAAAAAGTATAGGTTGTAGC
    AACCCTTGACCTGTACGAATGTTTATAAAACTTTCTATTTTCCTTAATTATATCTGTTTTGCATTTGTTT
    ATTTATTTATTTACTTACTTACTTATTTATGATTTCACTGTGTCTTTCTGTAGTCCTGTATGTGTTTATT
    TGTCTCTTTATTGCTCTGTTTCTGTTTCTTTCTCTCTGTCTAGTATTGTATCTCTCTGTGTCTCTATGTC
    GCCGATTGTGTCTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATATTGAAA
    TGAGATCTCTTCTTCTATCAGTGTACATAATGACTTTGAAATAAAGTCCATCACTGGGCTTGGATATCAC
    TATCTTGTTAGAATGGTTGCCCAAAGTGTTCCAACCATCACACTGTTTCTACATATAATAGATGGGTTTA
    TTGATTTACTACCAAAACTACCCCATCTTAAGTTATGGATATTAAACACAGATCCTCATGGTGTGTTAAG
    AACTTGAAGCACAGAGCTATATGTACAGCCCACTAAAAATGACTTAAATATAAGATAAACTCTGGCTTGA
    ACAAAACACAGAGCATCACACGAGGCAGAAGTGAATCTCTATGAGGACCAGGATCACATCCCAAAGAACC
    AAAGAACACTTGAGACTGTGGAACCCTAGAGTCAGGGCTGACACTCAGTAAGATTTACTTCATAGAGCAT
    GATGTGGGATTCAAGAAGAAATCAGGCAGGGCCTGTGACCCATGAACCAGATGACTTCCAGTCTCTCCAT
    CATCCTCACACAAACCATAGGCAACAGCTCAGCACTGACTCACCTGTGATGCTGGTGACAAAATGTGTGC
    AAAGCCTCAGAACTGAGACGTGAGGCCAATGTTGCTACCGACACTCAGTGAGCTTCAATTTGCTGCAAAC
    TGCTCCAAAACAACTCAAAGGGTAACTTCTTCTTTTTCTATTTGTACTTGTGATGCCTTGGTAGATATAG
    TTCCCCAATAATTTAATAGGACAAATCATTTTTCCTTTTAACACATTAATCCCCTTTCCTTCCCCCAAGA
    TTCTAAAATTTTTCTTTAACACTGATGTCTGAGAGATATGGCATAATAGATGTTTGTCTTATTCTGAGTT
    GTTCCTTATCAAAAGAAATCAGCTGTGGCACTTTGAACCTAAATATAACAATATTTCACAAAGGCATTCT
    GGGAATATATAGATGGTATTGCTACAAAAGAATAGGAGGATCTATGGCCATACCACCCTTAATGAACCAG
    ATACTATCTAAAAGATTAGGACAGCTGGGCATGTTGGCACAGGCCTTTAATCCCAGCACTAAGGAGGCAG
    AGGCAGGCAGATTTCTGATTCGAGGCCAGACTGGACTACAAAGTGAGTTCCAAGACAACCAGGGCTATAC
    AGAGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAGAGTAGGAGGGTATTAGATGCGAGGTGAT
    GATGGCCATTCAATCAAACCAGAATGTGACCTGAGAAAAGGTCTAAGTTTGTCAGCTTGTCCTGGAGATG
    TGTTGCCTCTGATAAATGCAGAGCTGGAACTGCAATACATTGGATTCCTGTGAGTTACGGAGAAATAAAA
    GGAATAGCCTCTGTTTCCTTTGGTTCATCAAGTTGGCAGTGAGCTAGAAGATGATACTGGGAACCATCAC
    ATGTACTTCTACAGAGTTATTCTACATTCTACAAGTCAGTCTTGCTTGCTTTCGTTTCTGTGAGGAGCTA
    AATACAGAATGTTTTCAATGTCTCATAATGTTCTGTGCACACCCATGGGTGCCTAGCCAAGGATAGAAAC
    TTGTATCTGATAAGGAAAGAGGATGCTGCAGTGGCCACACTCTGAGATCACCTCCTGCCAAATTATACTA
    GAGTGTTTTACATAGTCTTGAAAACACTAGGTCAAATATGTTGTCATCCAGATATGAAAACTCTGTGCAA
    CAATTCAGAAATACAGTGGTCAGGACAACTGGTATGTAACACTTTAGTTCCAGCCCACATTAATCTCACA
    TCTTTTCTGTGGTTTCAATAAAGAGCACACATGTTTGGAACACCATCTCCACTTTGTATGCTTATCCTTA
    TGCATCAAACTTTTCCTGTAATTTCCTACCCCGCACAGGTTCCTGTCTTGATCTCTTTTTCTAATGAACA
    GCAATACAGAAGTGTTGGCCAAATGAACACTTTCACCCCAAGTTGCTTTGCCATTGTGTTTCATCACAGC
    ATTAGTAACTCCCAGTAAGGCTACTGGTTACCCTGCATCTTCTATGTAGATCTTTTGATGAAGTTCCAGG
    CTTGGGGTCTTTATCTGACTGTTATCCTGGTTAATTCATGTCCACCAATAGAATATTGTTCTCATTTTCA
    ATATCATATGAAGTTACCTGTTTATAGGATCTTCCTAAAGCAGAAAACTATATAAAATGTCTGCTTACTT
    TAAGAAAAGTATGTGTCCTTGGAGGTAAATGGAGGAAAGGGTGGAGGATGTTGAGTAATGGAAGACTGGC
    TAATACAGGAAGGTCACAGAATTGAATGATTTTAAAAATTTTAACTCCTCATCAATGAGGTACTTCAAGT
    TGAGGCAGGAGTCCTTGATGCACAGGTGACCCAAAGGCCCCAGCCTCTGTTTCTTGGTCTGCTCCAGTCT
    CCCGGCTCAGCACAGTTCACTGAGTGACAATCTTTAAGATAGTCTTTCAGGATACAGTTATCACAGTTTT
    CAAACTCTCTTCATTGAAACAATATAATGAGTCAGAGTCCAGTGTGCCATTAATAGGCCCTATGCAATAG
    GATTTTACTTCATCTGTGGAAAGAAACCACCATGGCCTCATCAAGGCCTCATTTGATGATTAGTGTAGAA
    AGCGTCCCCAGAGCTTTGCTGGGATGAAAACCCTGGCAAGATGTTTAGGGCACTCTCCATCTGACAGGAC
    AATTCTATCAAGGGTTGCTTCCATATAGAGAGAAATCTCCACATCTGCAGTATTTCCCGATTTATCAACA
    GTTCTTTACCCCGTGTCATATGCATAAAATCTTTTCTCTGTAGAATTTTCAGTACTAAAAACTAACAAGG
    ATGTTGGTCCTGATATCAGACATAATAAATAAATAAACATCAAATTATACCCTAAACATGTGGAGATGGT
    TTTCTGTTATGAATTTATTGGCTCTCTAAAAAGTGTTAATTAAGCCAGGCGTGATGGCACATGCCTTTAA
    TCCCAGCACTTGTGAGGCAGAGGCAGGCGAATTTCTGAGTTTGAGGCCAAACTGATCTACAGAGTGATTT
    CCAGGACAGCCAGGGTTACACAAAAAAACCCTGTCTCCAAAAAGTAACAAAAAGAAAGTGTTAATTAACA
    TCTACACTTTAAGATAATTTGGTGGCAAAGGTTAATAGTCTGAAATACTAGATATGAAAGTATGACACCT
    AAATTGCATGCAGAGAATAAAGCTGAATTCCAATGAGATTCATGCCTTGTGAATCCATTCCAATCTGATC
    TCAATCTTCAAACATGTTTGTGCATAATTTACATCTTAATCAGTTGCTGGAAATTAAAAGGACATAAGCT
    AAACTTAAAACTCTTTCTATTTAATGTGTCAGAATAGAATATGTGCACTGAAGTTTTCATGTGCATAGGA
    TATCATTGTTACAATGATGGTTCAAATACGCACCAGTAGCACAGGTGATGCTTGACATGAAAAAAACTGT
    GACCAGAGCCCAGACGTTGATGTTGGCTGAAAGAGCCTAGTGTCTCACATTTGATCAAGCAAAGTGTTAT
    TCAGAGAAGCAGTGCCAGATTTGGGATCCTTCTTAATGGATCAAGAACATGAGGAAGATTTGGAAGCCAG
    ATTGATGAGATTACTAGAATCCTGGATCTGAACACACAACACACAATAGTAAGTTGAACTTTTCCACGCA
    ATGCTAAAGATAAAGCATCCAGTATCATGTTATACAGGACCAAGGTGAGGATCAGTCTGTGGGTGAAGTA
    CAATGAACTGGAAATTGTTTGGATTTTAAATGTTGGTTTTTAAATTATATATAAAATATATATATATATA
    TATATATATACATATATATATATATATATATATATATATATATATCGGAACCAACATTTAAAATTTGGAT
    TTTAAATGTTGGTTTTTAAATTATATATAAATTATATATATATATATATATATATATATATATATATATA
    TATATATATATATATCGGAACCAACATTTAAATTCCTAATTCTTAAGGACTTCACTTTTGACCAAAAATA
    TTTGAAATGGGTTATTTAGGTCTGTGGTGGTTTGAATATGCATAGCCTATAGGAAGTGGAAGTATGGCCA
    TTTTGTAGTATGTGTAGCCTTGATGGAGGAAGTACATCACTATATGGTGTTGTGAGAGTTGAGATCCTAT
    GTTCAAGTTCTGCCAAAAACAGAAGAGAGTCCCCTCCTGGATATTTGAAACAGATGCAGAGGCCCAAAGC
    CAAACATTGGGTATATCTCAGGGTTTCTTATGGAAAAGTTGGCTGAAGGATTGAAGGCCTTAAAGGGGAT
    AGGAACTTCACAGGAAAACCAACAGATTCATCTAACCTAGACTCTAGGGGTCTTTCAAAGGTTGAGTCAC
    CATACAAAAAGGACACAATGGCTAGACCTAGGTCCCTGATACATATGTAGCAAATGTGCAGCTTAGACTC
    CATGTGGATTCCCCAACGACTAGAGCAGGTATTGTCCCTGAAGTTATTGCTTGTCTGTAGAATACATTTC
    CCAACATAATTGTCTTGTCTGGCCTCAGTAGGAGAGTATGTTCCAATCCTGGAGAGACTTGATATGCCAG
    GATTGAGAGATTAAGGGTCTCCCACCATCTCAAAGTTCATGGGTATGACAGGGGAGGGGCAGTGCAGGTT
    TTCCCCATCTCTGATTTCCAGCATTTGCACCCCCAATATTGAGGGGGAGGTCTAGCAATTGGAGGGGGGA
    CAAACAGTATGAGAAAGCTAACTTTAGTGGTGTTGTCACAGTGTGGGGTATTGTGGGTGGGAGTGGAGAT
    GAATCCTCCTCCTCCTCAGGAGGCTGTAGCAGGTGCTTGCCCTCTTTTGGGACAGTAGAGCTGTGGTCAG
    TACTGGCCTGTTCCTGACAAATTTATTCTGTGCTGTGACCATTTATTCAAGGGAGGAAGATTTAAGACTT
    GCTATGATAACCTGACGAATCTGGACAAACAAGAGACAGGAGGTAACACCTGAGTTACTCTTTTGCTTGC
    TAGTACAGAAGAGCACTATAATCCACAAATTGGGGTCCCACATATTCTTGAGTGGGATCCAAGGTGCCAT
    AATTTCAGCCACATTCCAAAGTCCCTCAAGGTCTGACAGTTTAGTCTTTAGGCTTCTCCTAACCAAATCA
    TATTTGGAGTTCTTTAACATAAAAGGGTAGTTTCCCCATATCACTGCCAGCAAAACAGGTTGAATTTGGA
    CAGACAAGCAAAACTATCACAAACAAGACAGACTATGTGTGCGATCCAAATGTCTTGTGATTAGAGGGGA
    AGTTCTGCAGCTTATTAGGCTGTGTCCTTATGCCCACAAATGTCTTCAGATAGGAAAGGGCCATTGGGTG
    CCAATCCTTAGTTCTTCTGTTTCCTGGGCTGTCAGTTTTTATGTGGAGAAGACATGAACCAAATGCAAAG
    TCATGTATCCTACAAGAACTGACATACACAGACAAACAAGGAATAACAATGAGCTCTCTCATTCACCTAA
    GGGGGTCTTATAACATAGAACAGAAAGGGGCCAACTTAGCAAGAATTGACCAAAAGGTGGTCACCTAGAC
    CACACCACAATGCAGGAAGGATATCAATGAAGGAAGAACAAAGGAAAGGCCAAATCAAGTTTGTTGGCAC
    ATGACATAAAACAGTGCTCAAAAAACAATAGATGCGAGCAAAATGAAGCATTGGAATCATATCGGGGTAC
    ATAGGGGTCACTCTTACCCCTGTTCAAGTGCCAGATGTTGTTTTAGCCTTAGGCTCCCTGAGTTTGTCTT
    TAGGGGAAATGGGTAAGAATACACAAAAATAATGTCACAGAATCTTAGAGGCAGATGAGAAGTCAAAGCA
    GACTCATTTATTGCTATAGAAAAGAATGCCTTTATACCTTCTGTCCATAACACCTCTGCCCATATATGCT
    CATCTCTGTGAGGCAATGGACTACCTGCAGACAGGCTGGTATCCAGTCAAGCTGAGGTCTGAACCACAAT
    GGTCGTAACTCACCTAAAGGACGTGGTAGGCATGACGCGATAGGCGTTCCCTCATGCTCCTGGAACTCCG
    GCCCCTGCCTAAGGTACCACCTCCCACAGCCCCCACAAGAGAAGCATGGTCAGTAGTCATGTAAGCAATG
    GCCCAAGCTTCTGACCTTCAGCCTAAACTCCTCCCCAGTTACCTAGCAACAGTGAAGACCATAAAATGGG
    ATGTTAGGCCCCCACCTCACTCTCTTACTCCTTTGTTCCTTTACCTCTCACTTCTCTCAATTCTCTCTCC
    TCTTCCCTTTCTTTGTCTTCTTCTTTCCTTGTCTCTCTGTCTTTCTCTTTCTCTCTAGCCTTCTCTGCCT
    CTCTCTCTCTCTCTGTCTCTCTGTCTCTCTCTTCTCTCTTTCCCTTGAATTTCTTTTTTTCTTTTTTCTT
    TTTTTGATGAGCACAGTATAAATAGTTTATTGCATTGTTTCTGAATGTGGCTGTATTCTCTATTGTTCTC
    TTTCTTATTTTTTTTCTTTTTTTGGTTGTTTTTGTTTTTATAGTCTCGCACAGAATTCTACATCTACCAA
    ATCCCCATTGTGTACTTTAGTTTTGTCTTGTATTCAAAGGTTCTTTCAGAAAGAATCTTAAAGATGAAAT
    TTGCCTTCCAACTTTTCAAAAATACATATAAAGATAGATGTTACATAGTTCACGGAATAGACACATATAC
    GGAGATGCAGCAATATCTCCTTTGTGCCTCTTGTATATTCATGTTATTTCCTCAACTAAAAATACATAGC
    CACAAAGACATACAATGAGCACTCCACTTAGGAAAATATGGCCATATTTTTTTACATTTTTATGATTTTA
    TGATTAGGTATATTCTTCATTTACATTTCCAATGCTACCCCAAAAGTCCCCCAACCCTTCCCTCACTCCC
    CTTCCCCACTCCCACTTGTTGGCCCTGGAGTTCCCCTGTACTGAGGCATATAAAGTTTGCAAGACCAATG
    GGCCTCTCTTTCCACTGATGGCCCACAAGGTCATCTTCTGATACATATGCAGCTAGATACATATACATAC
    ACGAGCTCCAGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTATAGGGTTGCAGATCCCTTCAGCTC
    CTTGGGTACTTTCTCTAGCTCCTCCATTGAGGGCCCTGTGATCCATCCAATAGCTGACTGTGAGCATCCA
    CTTCTGTGTTTGCTAGGCCCCATTATAGCCTTGCAAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTT
    GCTAGTGTGTGCAATGGTGTCAGCATTTGGGGGCTGATTATGGCATGGATCCCCGGATATGGCAGTCTCT
    AGATGGTCCATCCTTTCATCTCAGCTCCAATCTTTGTCTCTGTAACTCTTTCCATGGGTGTTTTGTTCCC
    AATTCTAAGAAGGAGCAAAGTGTCCACACATTTGTATTCATTACTCTTGAGTTTCATGTGTTTCCCTTGC
    ATTTCTATAATAAACCATAAGGAGTCTCTGCTCTACCAAGACCCGCTGCACACTCTGGTCAGTGTTGGGA
    ACTTTTCCCCTATTCCCTCTCTCCTATAACTCCGGGGCTACAGGGTGTCTCCTTTGGGTCCCGGTTGGGA
    GCTGTCTCTTCTCAACCCCCTGACTCATGGGTCAGAGGCCTAAATCTCCACCCAAGGCTGTGTGAAAAGC
    ACCGGGTGGTCTCCCCATATCTCCCTGTCCAGAGCACAGGAACTCTGGCCGGACATGGCATATTTTTCCT
    CCCCAACTTCTTCCCCGGCCTCCTCAGGGCTGTCCCTTCATTTTGTTCCCCACACATCTCCACATGGCTG
    CCACCATCCAATTGGCTGCTGAGGTCACACACTCTGTCTTTGTTCTTCTCTGAGTCTCATGCAGGTTTTG
    TGTTAGTAAATGCAAAAGTGCCTGCTGTGCATGCATGAACCATTACAGGCTGCTAGGCAGAACATGCTGA
    CTTGCTCCTGGATTATAAATCCATCACTGTGATAGCAGGACATATCCAGAAGACATTATTTAACAACACT
    CCTCTTCGTGCTCAACCTCTCATGGTCTTTCTACCTCCTCTTTCTTAGTGTTCTCTGAGCCTACATGAAA
    TGTGAACAAACCTGAAAAAAATCTCTGTGTCAAATACATAGTGTGGATTGGGAAGGAAAAACCCTGCACT
    TGTGTGTGGGCAGGGAAATGCTGGTGCTTTAACAGGACAAGTGCGGTGTGGCTAGAGAAAGTGATGAGAA
    GAAGGGAAGCATGAGAACTGGGTGGGTGGATCCCATGTCTACTGAAAATGCTATGTTGATGTCAAGATAT
    TGTGTCTTGTTACAAAAATTCATTGAGTACCTAGGTTATTCTCAGTATATAAAAGGGGAGGAATGAAACT
    AGAATTAAACACACACACACACACACACACACACACACACAAACACACACACACGAGTAGAGAAAAGAAA
    TAGTCATGAGTTGCCTAGTGAATGAAGAGCGGAAAATGGTGCAGTTAATAGGGATCATAGAAAGAACTGG
    GGGATGCACACGAATGGGTTTCCTGTAATTATGTCTTCATAGTAATCTCTGCTCAATAATCAGACAGTGA
    CAATGTATGCCTCATTTACAAGCCCTGATGGCCTCTCAGAGGAAAGCATCTCTCTGAAATGAATAAAGTT
    CAGAAATGTCCTAAGTGTCCCTGTCACAGGAAAGCAGTGTTGGTAACGTTTCCAGGAAGCTCAGTCTTTG
    TCAAATATCCACAACAAGAAGAAGCCATGTCTAGACAGACAACAGACTGGGAAAGACTGAGCTCATGTAT
    GGAAACACAACTATGTGTCATGCTTTTATTTTCACATTATACTGAGGGGATATGACATTATAGAAACACA
    GTTTGTTCTTTGCATATGCTGAGAGAGGAACTAGATGAAGTGTGCTAATTCTTAGCAATAAAAAGATATA
    CAAAGTGGTACTTTACTGTTTCAATTTTTGTAACTGGCATCATTGTCTTTGATATTTTATATCTTCCTTG
    AGCACTGCCTTCTCTCAAGTGTCCAACTTCAGAGTATGCTATAGCAGGAAGACTACCAAATAAGATTAAT
    TTTTTTGTACCCATGAAAAAATTATGTGCCCTGACCCCTGTTCTCTGAAAGAGGAGCCAAGTTCTGGATT
    CCCAGATCCTCATATTCATTGATCAGCAGTGAACACAGATCATTCACCATGGACATGGGGCTCATCTGGA
    TTCTCCTTATTGTTTTTAAAAAAGGATTTCATTGGGAAAAGCTGCCTCATATTTCTATGACCAGGAGAGG
    AAGATACAGCTAGAGACACAAGCCCCACCATGTAAAATCTGTATGGTACTCTCGTTTTCATTTTACAACA
    TTTTGCTTTTGCATTGCAAAGGAGCAATAAGGGTTCAGCAATCAGCACTGTAACTGTACTTTTAAAATTT
    CCACCTCTACCCTCTGTGTCCTCTATCTAGTGATAGAAAGTGAAATAAGCACATAAAGCATGGGACAAGA
    AGAACACACAGAGGAGCAGAGACTGGCTACAAGTGGCAGCCAGCATGGAGCTGGACAGAATTTAAAAAGA
    TAAAGAGAAATGTCATGGCATGAAGCAGTGACTTTTTCTGTTCACAATGCCTGCAAAAACTTTATGGAGC
    CTACTACTGCTGAGATATGCAGGTTTGGTTCCAGAGAGGATTTTCTGTTTTATTTAATTTGCTTTTAAAT
    TTTTCTTCTTTGTATAGTCTGTATTTTTAAAAAGGGGTTGAATGAATACCTAAGCAGTTGGAAAATTTGT
    GAGTAGAAACCGAGGGCCTGAGAAACAAATAAATCAAAATTTTGAAATGGCAAAAGAAAGGGTTCATTCT
    ATTTTCTCTTTTCAGAAAAGGTTGCAAAGTTGGATGTGCAAATGGAGATGTTATGGGTGGACTTGGAACA
    TCTTGGGAAGGAAGGAACCCAGGAAAAGAAAGAATGAAATGGCTCAGCCTCAATGACTGAGCAAGAGAGT
    TAGAGTCACCCACAGGAGAAAAGAAGAAAATAAACAGCACTGGGGTGAATTTGGGTCAGAGAAAACCTTA
    AAATGCCAGAGGACAATGTGATGAAAGAGACCATTTGAAAGAAAGGGTTAACCTCATTCCATCAGTCATG
    CCAGAGTTAGAGAATGGGCAGTCTGGAGTTACAAGATCACGATTAGCCTTCCCAGTATGTATACAGCATA
    TTCCTGATAATGAGATGAAGCAAAGTTTGACAAGGTAAGATTGTATCCTATAGTAATATATAGATCAAAG
    GCATGTTGCAGTAAATCTCCAACAGAAATAAGCCCGGGCAATGAAAACACAACTCAATTAATATGAATAC
    GTGCTGTGCACCTAGACTGGGCAGATCTACCACTCCACTACCATATGAGAGATCCCTTATAACTTGTGGT
    TTCTCCAGGCCAGCTGCTTCTGCTCTACTTTCCTTCCTCCTCCTCCTATGTCATCCTCTCCCTCACTCTT
    TCTCTCCCAAAACTTTCAGCTGCACCTTCCCCTCTTCATCCCCCAATCAGTGGCTCTAGCCTTTATTTAT
    AAATTAAGGTAGGAAGAAGGTTTATAGGAAATTACCTGAGTGTTGACGTGTTGATAACCCCACACAAGAG
    AACAAAATTAATATCAAATATGATTAGCTCCAGGGTTATATGCAACAAAGGCATGATAAAGTGGTCATGA
    TTTCATATGAGATGTACTGGACTCATGTGAAAAAACGTTTAAATACATGGGCTTACTCAAAATATAGACT
    CATACCCCCAAGATGGGAAGAGACTGGTAACATTTGTACTGGAGGCTGGTCTGCTGTGGCAGCCATTAAC
    ACAGTGGAGGGAAGAGCCTCAAAGAGTGAAATAGAGGAAGGGGAACAAATACAGTAAACCACCAGTTACT
    ACTTAAAGGGCAGTGTTCTGATGTCCTAGGGAGGGCACTCCAAAATACCAATGGCCTTATCCTTAAATTT
    ATTGTTTAATGAGCCTACACTGATCTCAGTGGCCCACTACTAAGGAGAAATGACAGGCACTTGAACAGCT
    GATATGAGAACAGCTGGAGGCACAGTATACAGAAGAGTTTACAAGCCTATGGAACTCCCCTGTATTTTTT
    TATGATGAGGAAATCTGAAAAATGTAAAATGTTGAAAGATTTAAGAGCAGTTATTAGAGTAGATCAACCA
    ATGAGTCTCTTGCAGCCTGGAATTCTTTTATCTTGTTTGTAACAACAATCAGGCTCCATAAATTATAAAC
    TATAAAAATTTACTTTATAAATTATAAATATATATATATGTGTGTCTGTAGATAGATAGATAGATAAATA
    GATAGATAGATAGATAGATAGATAGATAGATATATGGATTTGATGGTTGCAGCAAATTCTAATTGGTTCC
    AACAACCCTACTCACATAATACATTCATACAGGTTTGATAGATGGAGCTAAGTTTTAAATAAATTATTTT
    TCTCCCATGGCTTATATATACACCACCAAAATTCTCAAAAATTAAAATGTGATTGTGTTGTATTTTTCTT
    TTACTGAATGACTATAAAAAGTTACAACATTCTCCATAAATTTACATGAAAAAATATTATGTAGTGCAGG
    TAAAGAAAACAAATTGACCCAAGAATGGTGTATATTCATTACTAAGCAACTTTTTAGATTCACAAAGTGT
    GGGTAAACAAGGTAATTTTTTCAATCAGTTTTTTTTAACTGGCAGGCAGCAATTCAGAGTTACAATGAGA
    AGATTAATAATTTTATTGTGTATTTTAAAATAAATCTTACAAAAATATTAATAGAATCACAAATTTATAC
    CTTTGTATAAAAACAATCAGTCATTTCTACTTTAAGAAACAGAACTCACATCTACTCATCAATCATTTTA
    TTAAGTCATATTACAAAGCTGAGTGCTAACATGGGTATAAGAAAACCATAACCTTATTCACCAGCCCAGC
    GTCAAAAAGAAAAAAACCAGTCATATCAGCTGCTGCTTCAAGAGTTCTTGTTCCTTGACATTAACAAAAT
    CCCTAGCTTAACTATTAAATTTTTTTTCAAAACTTCTAATTGATCCCTTAGATAAATGTTTGTGCTAACC
    ATCGGGACACATCCCATGAGTTCTGAAGCAGTGTGTTGTTCTTCATGCATGGCCCTTTTGTAGAGCTGTG
    AGTGTAGGGGAAGAGGGGGGAGAGAGAGCCCGTGTCCAGCCAGAGATCCTGTGCTCTGGGCAGGCAGACA
    CGGGAGGACAACGGAACACTTTTCACTCGGCCTTTGGTGGGCATCTGGCTGTGTGAAGTTACTGACCCCA
    CATGGTGGGGGATGGACAAGGGGCAGCCCCTGGTACCAGGAGCCCCAGGGCTACACTCTCGGCCCCAGAT
    ATACAAGAAGAGGGCAGAGGGAGAGAGGCTCCCACACAGGCGAGAGTCCTTAGTCTGGTCTGTGGCTGGA
    GCAGGGGAATTCCTTCTGATTGGAGATTAGGCACAGCTGATTAGGGGAAAGCCTACCCCATCATCCAAGC
    ACAATGGACTTTGAGGAACAGAGCCAGTCTAAGCTTTTATAGCTTTATGGTAGAAAGGCAGGGAGAAAGG
    AGAGAATGTGGAGAGAGACAGAGAGAGACTGGCCATGGCCAAGAGGAGGGAAGGGGGAAGAGAGAGAAAC
    AGGAAAAGCTAGAAAGTAAGATAAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAAAGTG
    GGGCCAAGCAGCCCCTTTTCTAGTGAGCTTGTCATCTCACAGTTGCTAGGTAACTGGGGAGGAGTTTAGT
    CTGAAGGTCAGAAGCTTGGGACCTTGTGTATGTGACTACTAACCACAGCTTCTCCTGTCGGGACTGTGGG
    AGCAGTAACTTTGACAGGAGACAGGGATCCAGGAGACATGAGGGAACACCTTCTGTCCCACATAGGTGGA
    ATCGGTTGTACCAGGGTTCAGAACTCAACTCCACTGGAGACCAGCCTATGTGTGCATAGCCCATTGCCCT
    ACAGCCTTTGACATTCTACAGAGTTCTGAGACTGGTAGAGATACAAAATAGCTAGGATTACACAGAGAAA
    CACTGGCTTGACAATAAAACTTCCAAAACAAATATAAATGAATAAATACACAAATTGGAGCAAGTGAACT
    TGCACTAAATCCATCAAAACTTGCACAGGATGGCTGTCTCCATAATGTTCAGATCTACTTCTATTAATGT
    CATTAAGAAGACAAAGATTATGCACAAAAAGAAACAAAACAAGAAACCCAAAAGGTAAAGGCTCCAATTG
    TAAGTAATAGACCCACAGTCTCTGAGGCCTGACTGTGCAGGTCCTCACCTTTGGTAATCAGCACTGAATG
    CAGACCACTCCTCATGGACTTTGGGCTCAGGTTTGTTTTCCTTGTCCTTATTTTAAAAGGTAATTCATAG
    AAATGAGATCCTGCCAGTATTGTGTACATGAGAAATAGAAAAATTGGTTTTCTTTGCTCTATTTTGTTTT
    GTTTGGTTAATGACAGTTTCCAAATCAGCATTCTTTGCTTTGAGGTGCCCAGTGTGAGGTGAAGCTGGTA
    GGGTCAGGGCAGCCTGGAGGGTCCCTGAAACACTCCTGTGCAGCCTCTGTAGTCACTGTGAGTGACTACT
    GAATGACCTGGGTCCTTCAGGCTCTAAAGAAGGGGCTGGAGAGGGTGGAAATAATTTTTAATGGTGGAGG
    TAGCACCTATTATCCAGACACCATGAAGGGCTGATTCACCATCTACAGAGATGATGCCAGAAACACACTT
    TACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
    TGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTATTCATATATATATATATAGTGTGTATGTATATTCATAT
    ATATATGTATATTCATAAGGTTGAGTAAATGGACTTAAACTGATTCCATCACAACTTCCATGGGATGGAT
    ATTTCCCAGTGTTAAGACCTGTCAACATCACTGTCATTCAGGAGACAATGATTATGCACAACAAAACAAG
    AAACCCAAAAGCAGAGGACTCCAATTACAATAGACCCAGACCCACAGTCTCTGAAGATTGACTGTACAGT
    TCAACCCAGCCCTGTACTTCTCTTCCCTAGAATTTACATTACTGAGTAACTGAGGAAAGCTCTACAATAT
    CTGTTCTCTATAGTGGTCAACACCTCCAAACACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTT
    CTTGCCATAATGTAGACTACTTCAGCCTATTTTGTACTCCAGTTAACGAAACTCAAGACTAGCTGCATGT
    TAGTCCTTATTCTGAAATATTATGAACAGGTGACCTCCCATCATTCACCAATGCAATAATCATATTTAGG
    AATATGAGGTTTTATGAGATATAAGCACAAGGGAGAGAAAGTAAGAAAACTACATAGATATATAGACTGA
    CATAAATCAAGACTTGCATGAGCTAGTGCCCAAGTACCATGTCCCTAAGTGGCAAGGAGTATCTTTTGAG
    CCTAGTGAGATGAGGTACAAATCGGACTCTTACATCTTTTTAGATAAACATGTGAGATCAATGGACTAAA
    GGCGTGAGCTGGGCTTCCTTGCAATCCATTTTCCATACAAGATAACAATAGATCTGGCTCCACAGACACG
    ATGAGAATAGTCTTAATTAGTTCTTTAAGTAGAATGACTGATCACTAAGAGCCCAATTCCATACTAAATA
    CTCTTCTGGATTATGCACAGATAAAAATTGCACATAGGGCATGGGGCACTGATCTCCCTGCACTACATGA
    ATGGGGGCTCATTTACTAAATGTTCCCATGTTTCTTCCTAGTGCTGCACAGAGCAAATCCTACAACTTCC
    TGCTTGTCTACAATGTAAACTCCAGACAGTACCAGAAAATCATTCCTTATGTTCCTCTCCAGGTGCTTCA
    ACAAGCACAGTGCAAATTTCTGTCACCCTG
  • Molecule Role : Vaximmutor
  • Additional Molecule Role : Vaximmutor
  • Related Vaccine(s): Streptococcus equi vicK mutant vaccine
IV. Vaccine Information
1. Equilis StrepE
a. Tradename:
Equilis StrepE
b. Manufacturer:
Intervet
c. Vaccine Ontology ID:
VO_0001054
d. Type:
Live, attenuated vaccine
e. Status:
Licensed
f. Host Species for Licensed Use:
Horse
g. Immunization Route
Intramuscular injection (i.m.)
h. Description
Live submucosal vaccine; deletions in aroA gene(Jacobs et al., 2000)
2. S. equi Subunit CNE Protein Vaccine
a. Vaccine Ontology ID:
VO_0011546
b. Type:
Subunit vaccine
c. Status:
Research
d. Gene Engineering of collagen binding, ancillary pilus subunit Cne
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
e. Adjuvant: Matrix-S
f. Immunization Route
Intranasally
g. Mouse Response
  • Host Strain: NMRI mice
  • Vaccination Protocol: Fifteen to twenty microgram of each antigen and 10 μg of the adjuvant in a total volume of 12–16 μl were given to the mice by placing the antigens onto the nares and allowing the mice to inhale. This was done at three occasions at 1-week intervals. In each experiment, control animals were given only adjuvant at the same concentration omitting the antigen (Flock et al., 2006).
  • Challenge Protocol: A dose containing 106 CFU in a 10-μl volume was used for infection of mice (Flock et al., 2006).
  • Efficacy: Mice immunized with recombinant CNE lost less weight and had less nasal colonization than the unvaccinated controls (Flock et al., 2006).
3. S. equi Subunit EAG, FNZ and SFS Protein Vaccine
a. Vaccine Ontology ID:
VO_0011544
b. Type:
Subunit vaccine
c. Status:
Research
d. Gene Engineering of binding protein Eag
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
e. Gene Engineering of Fnz
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
f. Gene Engineering of Sfs
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
g. Adjuvant: EtxB vaccine adjuvant
  • VO ID: VO_0001272
  • Description: The adjuvant used was EtxB, a recombinant form of the B subunit of Escherichia coli heat-labile enterotoxin (Flock et al., 2004).
h. Immunization Route
Subcutaneously
i. Mouse Response
  • Vaccination Protocol: Mice were immunized either subcutaneously (s.c.) or intranasally (i.n.). For each antigen (FNZ, SFS, and EAG), 12 μg of antigen was mixed with an equal amount of EtxB so that all components achieved a final concentration of 300 μg/ml. In the case of i.n. immunization, the total volume was kept to less than 40 μl and applied into the nostrils of anaesthetized mice . For both s.c. and i.n. immunizations, four doses were given with 1-week intervals (Flock et al., 2004).
  • Challenge Protocol: Challenge i.n. with S. equi subsp. equi (106 CFU in a 10-μl volume) was given 1 week after the last booster dose (Flock et al., 2004).
  • Efficacy: It was shown that nasal colonization of S. equi subsp. equi and weight loss due to infection were significantly reduced after vaccination compared with a mock-vaccinated control group. This effect was more pronounced after intranasal vaccination than after subcutaneous vaccination; nearly complete eradication of nasal colonization was obtained after intranasal vaccination (P < 0.001) (Flock et al., 2004).
  • Host Gene Response of IgA
    • Gene Response: An analysis of the bronchoalveolar lavage (BAL) fluid taken after the last immunization revealed significant IgA levels to EAG in the animals immunized with the three antigens and the EtxB adjuvant. By contrast, mice immunized with the three S. equi antigens in the absence of EtxB gave significantly lower IgA responses (Flock et al., 2004).
    • Detailed Gene Information: Click here.
4. Streptococcus Equi Live Culture Vaccine (USDA: 1831.00)
a. Manufacturer:
Wyeth
b. Vaccine Ontology ID:
VO_0001784
c. Type:
Live vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Horse
5. Streptococcus equi vicK mutant vaccine
a. Vaccine Ontology ID:
VO_0002798
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of vicK
  • Type: Gene mutation
  • Description: This vicK mutant is from Streptococcus equi (Liu et al., 2008).
  • Detailed Gene Information: Click here.
f. Immunization Route
intranasal immunization
g. Mouse Response
  • Persistence: A vicK mutant is attenuated in mice (Liu et al., 2008).
  • Efficacy: A vicK mutant induces protection in mice from challenge with wild type S. equi (Liu et al., 2008).
V. References
1. Chanter et al., 1999: Chanter N, Ward CL, Talbot NC, Flanagan JA, Binns M, Houghton SB, Smith KC, Mumford JA. Recombinant hyaluronate associated protein as a protective immunogen against Streptococcus equi and Streptococcus zooepidemicus challenge in mice. Microbial pathogenesis. 1999; 27(3); 133-143. [PubMed: 10455004].
2. Flock et al., 2004: Flock M, Jacobsson K, Frykberg L, Hirst TR, Franklin A, Guss B, Flock JI. Recombinant Streptococcus equi proteins protect mice in challenge experiments and induce immune response in horses. Infection and immunity. 2004; 72(6); 3228-3236. [PubMed: 15155624].
3. Flock et al., 2004: Flock M, Jacobsson K, Frykberg L, Hirst TR, Franklin A, Guss B, Flock JI. Recombinant Streptococcus equi proteins protect mice in challenge experiments and induce immune response in horses. Infection and immunity. 2004; 72(6); 3228-3236. [PubMed: 15155624].
4. Flock et al., 2006: Flock M, Karlström A, Lannergård J, Guss B, Flock JI. Protective effect of vaccination with recombinant proteins from Streptococcus equi subspecies equi in a strangles model in the mouse. Vaccine. 2006; 24(19); 4144-4151. [PubMed: 16580099].
5. Fu et al., 2013: Fu Q, Wei Z, Liu X, Xiao P, Lu Z, Chen Y. Glyceraldehyde-3-phosphate dehydrogenase, an immunogenic Streptococcus equi ssp. zooepidemicus adhesion protein and protective antigen. Journal of microbiology and biotechnology. 2013; 23(4); 579-585. [PubMed: 23568215].
6. Fu et al., 2013: Fu Q, Wei Z, Chen Y, Xiao P, Lu Z, Liu X. Identification of a surface protective antigen, CSP of Streptococcus equi ssp. zooepidemicus. Vaccine. 2013; 31(10); 1400-1405. [PubMed: 23306366].
7. Jacobs et al., 2000: Jacobs AA, Goovaerts D, Nuijten PJ, Theelen RP, Hartford OM, Foster TJ. Investigations towards an efficacious and safe strangles vaccine: submucosal vaccination with a live attenuated Streptococcus equi. The Veterinary record. 2000; 147(20); 563-567. [PubMed: 11104039].
8. Lin et al., 2011: Lin HX, Huang DY, Wang Y, Lu CP, Fan HJ. A novel vaccine against Streptococcus equi ssp. zooepidemicus infections: the recombinant swinepox virus expressing M-like protein. Vaccine. 2011; 29(40); 7027-7034. [PubMed: 21807055].
9. Liu et al., 2008: Liu M, McClure MJ, Zhu H, Xie G, Lei B. The Two-Component Regulatory System VicRK is Important to Virulence of Streptococcus equi Subspecies equi. The open microbiology journal. 2008; 2; 89-93. [PubMed: 19088917].
10. Meehan et al., 1998: Meehan M, Nowlan P, Owen P. Affinity purification and characterization of a fibrinogen-binding protein complex which protects mice against lethal challenge with Streptococcus equi subsp. equi. Microbiology (Reading, England). 1998; 144 ( Pt 4); 993-991003. [PubMed: 9579073].
11. MicrobeWiki: S. zooepidemicus: MicrobeWiki: S. zooepidemicus [http://microbewiki.kenyon.edu/index.php/Streptococcus_zooepidemicus]
12. Nally et al., 2000: Nally JE, Artiushin S, Sheoran AS, Burns PJ, Simon B, Gilley RM, Gibson J, Sullivan S, Timoney JF. Induction of mucosal and systemic antibody specific for SeMF3 of Streptococcus equi by intranasal vaccination using a sucrose acetate isobutyrate based delivery system. Vaccine. 2000; 19(4-5); 492-497. [PubMed: 11027813].
13. Timoney, 2004: Timoney JF. The pathogenic equine streptococci. Veterinary research. 2004; 35(4); 397-409. [PubMed: 15236673].
14. Velineni and Timoney, 2013: Velineni S, Timoney JF. Characterization and protective immunogenicity of the SzM protein of Streptococcus zooepidemicus NC78 from a clonal outbreak of equine respiratory disease. Clinical and vaccine immunology : CVI. 2013; 20(8); 1181-1188. [PubMed: 23740925].
15. Waller et al., 2007: Waller A, Flock M, Smith K, Robinson C, Mitchell Z, Karlstrm A, Lannergrd J, Bergman R, Guss B, Flock JI. Vaccination of horses against strangles using recombinant antigens from Streptococcus equi. Vaccine. 2007; 25(18); 3629-3635. [PubMed: 17321016].
16. Wei et al., 2012: Wei Z, Fu Q, Liu X, Xiao P, Lu Z, Chen Y. Identification of Streptococcus equi ssp. zooepidemicus surface associated proteins by enzymatic shaving. Veterinary microbiology. 2012; 159(3-4); 519-525. [PubMed: 22613253].
17. Wei et al., 2013: Wei Z, Fu Q, Chen Y, Li M, Cong P, Mo D, Liu X. Streptococcus equi ssp. zooepidemicus C5a peptidase, a putative invasin, induces protective immune response in mice. Research in veterinary science. 2013; 95(2); 444-450. [PubMed: 23632199].
18. Wiki: Strangles: Strangles [http://en.wikipedia.org/wiki/Streptococcus_equi]