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Toxoplasma gondii

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Disease
    3. Introduction
    4. Microbial Pathogenesis
    5. Host Ranges and Animal Models
  2. Vaccine Related Pathogen Genes
    1. dasd
    2. fad
    3. fsa
    4. sdfa
    5. BiP (Protective antigen)
    6. GRA1 (Protective antigen)
    7. GRA2 (Protective antigen)
    8. GRA5 (Protective antigen)
    9. hsp70 (Protective antigen)
    10. MIC13 (Protective antigen)
    11. MIC3 (Protective antigen)
    12. MIC3 (Protective antigen)
    13. MIC8 (Protective antigen)
    14. p30 (Protective antigen)
    15. PI1 (Protective antigen)
    16. Rop2 (Protective antigen)
    17. ROP2 (Protective antigen)
    18. Rop2 (Protective antigen)
    19. ROP8 (Protective antigen)
    20. SAG1 (Protective antigen)
    21. SAG2 (Protective antigen)
    22. SOD (Protective antigen)
    23. mic2 (Virmugen)
    24. OMPDC (Virmugen)
    25. UP (Virmugen)
  3. Vaccine Related Host Genes
    1. Ccl2
    2. Ifng (Interferon gamma)
    3. IL-6
    4. Il12b
    5. TNF-alpha
  4. Vaccine Information
    1. Ovilis Toxovax
    2. rBCGpMV361-TgCyP
    3. T. gondii DNA vaccine encoding MIC8
    4. T. gondii DNA vaccine pcDNA3-MIC3
    5. T. gondii DNA vaccine pcDNA3.1-SAG1-ROP2
    6. T. gondii DNA vaccine pMIC3i encoding the complete MIC3-ORF (RH(ERP) strain)
    7. T. gondii DNA vaccine pSAG1-ROP2-SAG2
    8. T. gondii DNA vaccine pVAX-TgMIC13
    9. T. gondii DNA vaccine pVR1020-GRA1
    10. T. gondii DNA vaccine ROP8-pVAX-1
    11. T. gondii DNA vaccine T.g.HSP70
    12. T. gondii recombinant vaccine vector rBCGpMV361-TgCyP
    13. T. gondii Subunit Rop2-Hsp83 Fusion Protein Vaccine
    14. Toxoplasma gondii mic2 mutant vaccine
    15. Toxoplasma gondii OMPDC mutant vaccine
    16. Toxoplasma gondii OMPDC/UP mutant vaccine
  5. References
I. General Information
1. NCBI Taxonomy ID:
5811
2. Disease:
Toxoplasmosis
3. Introduction
Toxoplasma gondii is a species of parasitic protozoa in the genus Toxoplasma. The definitive host of T. gondii is the cat, but the parasite can be carried by many warm-blooded animals (birds or mammals). Toxoplasmosis, the disease of which T. gondii is the causative agent, is usually minor and self-limiting but can have serious or even fatal effects on a fetus whose mother first contracts the disease during pregnancy or on an immunocompromised human or cat (Wiki: T. gondii).
4. Microbial Pathogenesis
T. gondii is an intestinal coccidian of cat with man as one of the intermediate host. Humans are generally infected by ingesting oocysts released in cat faeces or by consuming meat from infected herd animals containing the long lived tissue cysts. Following ingestion, the outer wall of cysts or oocysts are disrupted by enzymatic degradation and bradyzoites and sporozoites are liberated into intestinal lumen. They rapidly invade and multiply within surrounding cells where they become tachyzoites. Thereafter, tachyzoites are disseminated via blood or lymphatic system to most of the organs of human body. At these sites, the tachyzoites infect host cells, replicate and invade the adjoining cell. In this fashion, the hallmarks of the infection develop: cell death and focal necrosis surrounded by an acute inflammatory response (Bhopale, 2003).
5. Host Ranges and Animal Models
The life cycle of T. gondii has two phases. The sexual part of the life cycle (coccidia like) takes place only in members of the Felidae family (domestic and wild cats), which makes these animals the parasite's primary host. The asexual part of the life cycle can take place in any warm-blooded animal, like other mammals (including felines) and birds (Wiki: T. gondii).
1. BiP
  • Gene Name : BiP
  • Sequence Strain (Species/Organism) : Toxoplasma gondii
  • NCBI Protein GI : AAF23321
  • Other Database IDs : CDD:331774
  • Taxonomy ID : 5811
  • Protein Name : heat shock protein 70 precursor
  • Protein pI : 5
  • Protein Weight : 67889.46
  • Protein Length : 743
  • Protein Note : immunoglobulin binding protein
  • Protein Sequence : Show Sequence
    >AAF23321.1 heat shock protein 70 precursor [Toxoplasma gondii]
    MTAAKKLSLFSLAALFCLLSVATLRPVAASDAEEGKVKDVVIGIDLGTTYSCVGVYRHGRVDIIPNDQGN
    RITPSYVAFTDDDRKIGEAAKNEATINPTNTLFDVKRLIGRRFNEKEVQKDKDLLPYEIINKDGKPYIRV
    MVKGEPKVLAPEEVSAMVLTKMKETAEQFLGKEVKNAVVTVPAYFNDAQRQATKDAGAIAGLNVIRIINE
    PTAAAIAYGLDKKNEKTILVYDLGGGTFDVSVLVIDNGVFEVLATSGDTHLGGEDFDQRVMDHFIKLVKK
    KYDKDLRTDKRGLQKLRREVERAKRALSSQHQAKVEVENLMEGVDFSETLTRAKFEELNSDLFQKTLKPV
    KQVLEDADLQKSQVDEIVLVGGSTRIPKIQQLIKDFFNGKEPNRGINPDEAVAYGAAVQAGILSGEGAQD
    MVLLDVTPLTLGIETAGGVMAKIINKNTVIPTKKTQTFSTYSDNQSAVLIQVYEGERPMTKHNHLLGKFE
    LTGIPPAPRGVPQIEVTFDVDRNGILSVSAVDKGTGKSEKITITNDKGRLTPEEIERMISEAEKFAEEDK
    KVKERVDARNALEGYLHSMKTTVEDKDKLADKIEEDDKKTILDKVTEAQEWLNTNPDADAEETRDKLKDV
    EAVCNPIISKVYGQSGGPGAGGAAGGADDDDYGGHDEL
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Czarnewski et al., 2017)
2. dasd
  • Gene Name : dasd
  • Molecule Role Annotation : Oral immunization of mice with ZJ111/pcDNA3-SAG1 elicited specific humoral responses and stimulated proliferation of splenocytes (P<0.05). Mice immunized with ZJ111/pcDNA3-SAG1 displayed significant protection against an intraperitoneally challenge with 500 tachyzoite forms of T. gondii RH strain. Vaccination at 107 and 108 CFU per mice provided a 20% and 10% survival rate comparing 100% mortality of the non-immunized mice, exhibiting longer living time and better survival rate (Qu et al., 2008).
3. fad
  • Gene Name : fad
4. fsa
  • Gene Name : fsa
5. GRA1
  • Gene Name : GRA1
  • Sequence Strain (Species/Organism) : Toxoplasma gondii
  • NCBI Protein GI : 301750620
  • Taxonomy ID : 5811
  • Gene Strand (Orientation) : ?
  • Protein Name : P24 granule antigen
  • Protein pI : 3.92
  • Protein Weight : 18678.03
  • Protein Length : 246
  • Protein Sequence : Show Sequence
    >ADK88943.1 P24 granule antigen [Toxoplasma gondii]
    MVRVSAIVGAAASVFVCLSAGAYAAEGGDNQSSAVSDRASLFGLLSGGTGQGLGIGESVDLEMMGNTYRV
    ERPTGNPDLLKIAIKASDGSYSEVGNVNVEEVIDTMKSMQRDEDIFLRALNKGETVEEAIEDVAQAEGLN
    SEQTLQLEDAVSAVASVVQDEMKVIDDVQQLEKDKQQLKDDIGFLTGERE
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): T. gondii DNA vaccine pVR1020-GRA1
6. GRA2
  • Gene Name : GRA2
  • Sequence Strain (Species/Organism) : Toxoplasma gondii
  • NCBI Protein GI : EPT27242
  • Taxonomy ID : 508771
  • Protein Name : dense granule protein GRA2
  • Protein pI : 9.71
  • Protein Weight : 18298.13
  • Protein Length : 253
  • Protein Note : encoded by transcript TGME49_227620;
    Signal peptide predicted by SignalP 2.0 HMM (probability 0.997) with cleavage site probability 0.917 at residue 23;
    GO_component: GO:0020003 - symbiont-containing vacuole;ev_code=EXP;
    GO_component: GO:0045177 - apical part of cell;ev_code=EXP
  • Protein Sequence : Show Sequence
    >EPT27242.1 dense granule protein GRA2 [Toxoplasma gondii ME49]
    MFAVKHCLLVVAVGALVNVSVRAAEFSGVVNQGPVDVPFSGKPLDERAVGGKGEHTPPLPDERQQEPEEP
    VSQRASRVAEQLFRKFLKFAENVGQHSEKAFKKAKVVAEKGFTAAKTHTVRGFKVAKEAAGRGMVTVGKK
    LANVESDRSTTTTQAPDSPNGLAETQAPVEPQQRAAHVPVPDFSQ
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Ching et al., 2017)
7. GRA5
  • Gene Name : GRA5
  • Sequence Strain (Species/Organism) : Toxoplasma gondii
  • NCBI Protein GI : EPT31025
  • Taxonomy ID : 508771
  • Protein Name : dense granule protein GRA5
  • Protein pI : 5.01
  • Protein Weight : 12026.38
  • Protein Length : 187
  • Protein Note : encoded by transcript TGME49_286450;
    Signal peptide predicted by SignalP 2.0 HMM (probability 0.923) with cleavage site probability 0.441 at residue 26;
    Predicted trans-membrane domain (TMHMM2.0):6-26:70-93;
    GO_component: GO:0020003 - symbiont-containing vacuole;ev_code=EXP;
    GO_component: GO:0045177 - apical part of cell;ev_code=EXP
  • Protein Sequence : Show Sequence
    >EPT31025.1 dense granule protein GRA5 [Toxoplasma gondii ME49]
    MASVKRVVVAVMIVNVLALIFVGVAGSTRDTGSGGDDSEGAWGGEQQQVQQHGQSEDRSLFERGRAAVTG
    HPVRTAVGLAAAVVAVVSLLRLLRRRRRRAIQEESKESATAEEEEVAEEE
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Ching et al., 2017)
8. hsp70
  • Gene Name : hsp70
  • Sequence Strain (Species/Organism) : Toxoplasma gondii
  • NCBI Protein GI : 12248795
  • Other Database IDs : CDD:240227
    CDD:212675
    CDD:295646
  • Taxonomy ID : 5811
  • Gene Strand (Orientation) : ?
  • Protein pI : 5.38
  • Protein Weight : 69239.84
  • Protein Length : 723
  • Protein Note : heat shock 70 kDa protein; Provisional
  • Protein Sequence : Show Sequence
    >BAB20284.1 hsp70 [Toxoplasma gondii]
    MGSSHHHHHHSSGLVPRGSHMLEMADSPAVGIDLGTTYSCVGVWKNDAVEIIANDQGNRTTPSYVAFTDT
    ERLVGDAAKNQVARNPENTIFDAKRLIGRKFDDPSVQSDMKHWPFKVIAGPGDKPLIEVTYQGEKKTFHP
    EEVSAMVLGKMKEIAEAYLGKEVKEAVITVLAYFNDSQRQATKDAGTIAGLSVLRIINEPTAAAIAYGLD
    KKGCGEMNVLIFDMGGGTFDVSLLTIEDGIFEVKATAGDTHLGGEDFDNRLVDFCVQDFKRKNRGKDIST
    NSRALRRLRTQCERTKRTLSSSIQATIEIDSLFEGIDYSVSISRARFEELCMDYFRNSLLPVEKVLKDSG
    IDKRSVSEVVLVGGSTRIPKIQQLITDFFNGKEPCRSINPDEAVAYGAAVQAAILKGVTSSQVQDLLLLD
    VALLSLGLETAGGVMTKLIERNTTIPTKKSQTFTTYADNQPGVLIQVYEGERAMTKDNNLLGKFHLDGIP
    PAPRGVPQIEVTFDIDANGIMNVTAQDKSTGKSNQITITNDKGRLSASEIDRMVQEAEKYKAEDEQNKHR
    VEAKNGLENYCYHMRQTLDDEKLKDKISSEDRDTANKAIQEALDWLDKNQLAEKEEFEAKQKEVESVCTP
    IITKLYQAGAAAGGMPGGMGGMPGGMGGMPGGMGGMPGGMGSGC
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): T. gondii DNA vaccine T.g.HSP70
9. MIC13
  • Gene Name : MIC13
  • Sequence Strain (Species/Organism) : Toxoplasma gondii
  • NCBI Protein GI : 380449403
  • Other Database IDs : CDD:287526
  • Taxonomy ID : 5811
  • Gene Strand (Orientation) : ?
  • Protein Name : microneme protein 13
  • Protein pI : 6.48
  • Protein Weight : 49684.02
  • Protein Length : 529
  • Protein Note : Sialic-acid binding micronemal adhesive repeat; pfam10564
  • Protein Sequence : Show Sequence
    >AFD54637.1 microneme protein 13 [Toxoplasma gondii]
    MVGVFQLCLLTCLLCLLKGSDAIQLRQHRNGLGVEQSFVSEETDIVGGRISSLQAELDSYCHNRYKQLCD
    GGNKNFCDKTGVARYGTGIQSQQTAEWRCYFAEVLKPSGRKVQCVDDCGNYFPCLGVVDPNDTFHATAHN
    QILNFIKDGVEKHCSPFQMAGNNYCQGVLADTVARKDTGTASQKAKAWRCYKKDSLTYEARSICVDNCGG
    EIECPGGRSETSGELLSQHSTREKELLKVIESQSAPCRDSCVATPINPPICADTESLLQSTKRSRAQRFI
    DDACQKLFNCHCKKDKKFCTTVVARKDRGRAGSQNDAEWRCYSLEELDFQKTNPSCIDECGNELPCQGAV
    PEESTHHITWTKLPSKIDEATQKFCTERQRAANDYCASEFADSLARYGPGAGDQTSQFRCIKKAAMRADA
    EGKCADSCKGTETCGGCRSRLDGPLSVNDVLNAHEGISEAMKSASTEC
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): T. gondii DNA vaccine pVAX-TgMIC13
10. mic2
  • Gene Name : mic2
  • NCBI Protein GI : 1923217
  • 3D structure: PDB ID : 2XGG
  • Other Database IDs : CDD:29244
    CDD:154951
  • Taxonomy ID : 5811
  • Gene Strand (Orientation) : ?
  • Protein Name : micronemal protein MIC2
  • Protein Length : 769
  • Protein Note : Micronemal proteins: The Toxoplasma lytic cycle begins when the parasite actively invades a target cell. In association with invasion, T. gondii sequentially discharges three sets of secretory organelles beginning with the micronemes, which contain...; cd01471
  • Protein Sequence : Show Sequence
    >gi|1923217|gb|AAB63303.1| micronemal protein MIC2 [Toxoplasma gondii]
    MRLQREAVFGLMFACGMWMWPSEVAGGGWSIVDALRKRYDTSRGGDANGVDTSGVEDVIQSDSAIGAAEG
    CTNQLDICFLIDSSGSIGIQNFRLVKQFLHTFLMVLPIGPEEVNNAVVTYSTDVHLQWDLQSPNAVDKQL
    AAHAVLEMPYKKGSTNTSDGLKACKQILFTGSRPGREHVPKLVIGMTDGESDSDFRTVRAAKEIRELGGI
    VTVLAVGHYVKHSECRSMCGCSGTSDDDSPCPLYLRADWGQLATAIKPMLKEVCKTLPQDAICSDWSAWS
    PCSVSCGDGSQIRTRTEVSAPQPGTPTCPDCPAPMGRTCVEQGGLEEIRECSAGVCAVDAGCGVWGEWSA
    WSASCGNATRKRERTRYNDPPPQGAGRRCENQDPPVLQEQTEEATLAPCITIPPTPPEWAAWSDCTVTCG
    GGNRHRVRNALPPGLGSQNGESDESLVSKLWPGTDLRQEEACNTSPCPINATCGQFEEWSTCSVSCGGGL
    KTRSRNPWNEDQQHGGLSCEQQHPGGRTETVTCNPQACPVDERPGEWAEWGECSVTCGDGVRERRRGKSL
    VEAKFGGRTIDQQNEALPEDLKIKNVEYEPCSYPACGASCTYVWSDWNKCVCPMGYQARHAAVKFDYRNK
    PCDLPTFETKACSCGETNPVPSEGTTPGAPGATGPEQPNQRPLPEGSDENETPTNEEGEQSKKESGSGIA
    GAIAGGVIGGLILLGAAGGASYHYYLSSSVGSPSAEIEYEADDGATKVVMEEEKETLVPVDDDSDMWME
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A mic2 mutant is attenuated in mice and induces protection from challenge with wild type T. gondii (Huynh and Carruthers, 2006).
  • Related Vaccine(s): Toxoplasma gondii mic2 mutant vaccine
11. MIC3
  • Gene Name : MIC3
  • Sequence Strain (Species/Organism) : Toxoplasma gondii ME49
  • VO ID : VO_0011174
  • NCBI Gene ID : 7901253
  • NCBI Protein GI : 1085201908
  • Locus Tag : TGME49_325800
  • Genbank Accession : KE139555
  • Protein Accession : XP_002369833
  • Taxonomy ID : 508771
  • Chromosome No : Unknown
  • Gene Starting Position : 8
  • Gene Ending Position : 917
  • Gene Strand (Orientation) : -
  • Protein pI : 6.46
  • Protein Weight : 26732.05
  • Protein Length : 269
  • DNA Sequence : Show Sequence
    >NW_017383630.1:8-917 Toxoplasma gondii ME49 unplaced genomic scaffold asmbl.1555, whole genome shotgun sequence
    CTCACGTGAGTACCCTGGTGGGGCACTCGCAGGTGTAGCCATTCTCATGCGAGGCGTCATCAATACATCT
    ACCAGGGCTGCAGTTCTCCCTGAAGGCATGACATCCAGTCCTCTTGCATCCTTGTCCCCCTTCCGGGAGG
    TTCACTAGAGTTTCGCCGTCGCCGCAGGTGCATGTGTAGCTGACTGAATCGACGACGATGCACGTCCCGT
    TGGGTCCGCACTTCGCGTTCCCTCTTTTTGAACAAGGATCCTCGGAGCAAGTCAACCCAGTGCCGACGAA
    GCCGTCTTTGCACTTGCAGTGCACACCCCCACCCGCGTCCAAGTTTTCGACGCATTCTGCGTTTTTGTCG
    CACCGATGGCCCTGCTTTTCACAGCTGCAGTCTCCTCCATAGCTTTTGTCAGGATGGTAGATGAGCGAAC
    TGCTGCAAAAAATCCAGTTGCTGGAGCAGAATTCTCCGCAGCCGCCGGGGCCGCACATGGTCTTGCTGCA
    GCAAGCGCTCTTGGGGCAACTGGCCCCGATGAAGTACCCGTTGTCAGTGTGCAACTGACGGTTTCGACAT
    GGCTTGCCTTCACTCGAGATAGCACAGAGTTGCGTCTCCTGCTTGCTGGGGGATTGCACAGACGAGTGAG
    TCTCAGTCACCGCGAAAGACGGAGCAAGACTCTCAGAGACGACTTCGCGGCTCGGCACAACTTTGTCAAA
    GCTGCCCAGCTGCACAGACTTCTGAATCGGCAAAGCCTCCGCTGGGGTGCACATCCACACGGCCCCACTG
    AAGGTGAGCGCGTGCAACAGCGCGGACGTCCCGCCTCGCATTTTTACCAGTGTTGGACAAGGAAGTTACA
    CACAGAGTAAGGCGCGCAAACGCGCTGCACTGCCCACAGAAATGCACAGAAACGCACTCTCGACGAGCTG
    
    
  • Protein Sequence : Show Sequence
    >XP_002369833.2 EGF family domain-containing protein [Toxoplasma gondii ME49]
    MRGGTSALLHALTFSGAVWMCTPAEALPIQKSVQLGSFDKVVPSREVVSESLAPSFAVTETHSSVQSPSK
    QETQLCAISSEGKPCRNRQLHTDNGYFIGASCPKSACCSKTMCGPGGCGEFCSSNWIFCSSSLIYHPDKS
    YGGDCSCEKQGHRCDKNAECVENLDAGGGVHCKCKDGFVGTGLTCSEDPCSKRGNAKCGPNGTCIVVDSV
    SYTCTCGDGETLVNLPEGGQGCKRTGCHAFRENCSPGRCIDDASHENGYTCECPTRVLT
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Protective experiments of DNA vaccine pcDNA3-MIC3 (pMIC3i) in Kumming mice showed that the vaccine could significantly prolong the survival time of the mice challenged by virulent RH strains of T. gondii (Xiang et al., 2009).
  • Related Vaccine(s): T. gondii DNA vaccine pcDNA3-MIC3
12. MIC3
  • Gene Name : MIC3
  • Sequence Strain (Species/Organism) : Toxoplasma gondii strain RH(ERP)
  • NCBI Nucleotide GI : 5931753
  • NCBI Protein GI : 5931754
  • Protein Accession : CAB56644.1
  • Other Database IDs : CDD:304395
    CDD:283826
    InterPro: IPR000742
    InterPro: IPR006210
    UniProtKB/TrEMBL: Q9GRG4
  • Taxonomy ID : 5811
  • Gene Strand (Orientation) : ?
  • Protein Name : MIC3 microneme protein
  • Protein pI : 6.42
  • Protein Weight : 36421.86
  • Protein Length : 421
  • Protein Note : Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the...; cl21504
  • DNA Sequence : Show Sequence
    >gi|5931753|emb|AJ132530.1| Toxoplasma gondii MIC3 gene
    CCGCTGTCCCGCCTGGTCAACAAGTATCACACCCTCGTCCCCGCCATTGGCACGGAGCTCGATGAGCTGC
    AGTGTCGCTTTTAGGGGAGTCGTGCAATCACGCCGCAACACAGGCGTGATTCGATCTTCAATTGCTAGGT
    AACCACTCGTGCTTGGTAGCTCTGCAATGGCTCGAGCGACGGGGGTGATGCAACATGCTGCTAAAAACTC
    GACAGACGTGTCACCGGAACCCACCTAAATAGGAGACCACGGGTCTCTGGTGTGTCGCGTCGCATTCTCG
    CGTCGCATTCTCGCGTCGCAATGACCGGCCAGTTGCTCGACGTCGCCAGCCGGGACTGAAGAGCGTTCAT
    CGAGTCAGCAGCATTGCGTCCCCTTGCTCGGTGAAAAAAGACTCTCTGGTCGAGTCTAGCTCGTGTCACT
    TCTGTTTCTAACCTCCTTCGTTCACCGGTACACCTCCGATGTGACTTTTGGTACACTTGCCCTGTCGCAC
    GACGCACGCTGTCACTCAACTTGCTGCTAGCGCAATCGATAGGTTCCCTCGAACCAGCCATCACACACAC
    ACCTTTTCCGGGAAGACGTTTGCGGGCGGTGGGTCGCAGCTCGTCGAGAGTGCGTTTCTGTGCATTTCTG
    TGGGCAGTGCAGCGCGTTTGCGCGCCTTACTCTGTGTGTAACTTCCTTGTCCAACACTGGTAAAAATGCG
    AGGCGGGACGTCCGCGCTGTTGCACGCGCTCACCTTCAGTGGGGCCGTGTGGATGTGCACCCCAGCGGAG
    GCTTTGCCGATTCAGAAGTCTGTGCAGCTGGGCAGCTTTGACAAAGTTGTGCCGAGCCGCGAAGTCGTCT
    CTGAGAGTCTTGCTCCGTCTTTCGCGGTGACTGAGACTCACTCGTCTGTGCAATCCCCCAGCAAGCAGGA
    GACGCAACTCTGTGCTATCTCGAGTGAAGGCAAGCCATGTCGAAACCGTCAGTTGCACACTGACAACGGG
    TACTTCATCGGGGCCAGTTGCCCCAAGAGCGCTTGCTGCAGCAAGACCATGTGCGGCCCCGGCGGCTGCG
    GAGAATTCTGCTCCAGCAACTGGATTTTTTGCAGCAGTTCGCTCATCTACCATCCTGACAAAAGCTATGG
    AGGAGACTGCAGCTGTGAAAAGCAGGGCCATCGGTGCGACAAAAACGCAGAATGCGTCGAAAACTTGGAC
    GCGGGTGGGGGTGTGCACTGCAAGTGCAAAGACGGCTTCGTCGGCACTGGGTTGACTTGCTCCGAGGATC
    CTTGTTCAAAAAGAGGGAACGCGAAGTGCGGACCCAACGGGACGTGCATCGTCGTCGATTCAGTCAGCTA
    CACATGCACCTGCGGCGACGGCGAAACTCTAGTGAACCTCCCGGAAGGGGGACAAGGATGCAAGAGGACT
    GGATGTCATGCCTTCAGGGAGAACTGCAGCCCTGGTAGATGTATTGATGACGCCTCGCATGAGAATGGCT
    ACACCTGCGAGTGCCCCACAGGGTACTCACGTGAGGTGACTTCCAAGGCGGAGGAGTCGTGTGTGGAAGG
    AGTCGAAGTCACGCTGGCTGAGAAATGCGAGAAGGAATTCGGCATCAGCGCGTCATCCTGCAAATGCGAT
    AACGGATACTCCGGATCTGCTTCCGCAACCTCCCACCATGGGAAAGGAGAATCGGGATCCGAGGGGAGCT
    TGAGTGAAAAAATGAATATTGTCTTCAAGTGCCCCAGTGGCTACCATCCAAGATACCATGCCCACACCGT
    GACGTGTGAGAAAATTAAGCAGTGAGCTGGAGTCTGTCCTTTACTTTAGCATTGGAGAATGCGCCCATTC
    GCAGCTGTCCGTGCATGCAATCATCGCCGTCTGTATAAAACCGACACAAACTGACCTTTATGTAACTGTA
    CAGCTTTGCCCTTGACGGGGCCGGCAACCACGACACGACTACGTATGTCGCAAGACGAAGGTACCCAGCG
    AGTCTCTGAGAGCGGAGATCAGCGCAAAGACAAGATGCAGAGTTTGACTCGAGAAACAATAGTAACACGA
    AGTAAAAAGTCTCCACACTAAGCCAAGGATTGAGAATATTTCGATTTGTGCCGCTGGCAATAGTGGCCTT
    GGCTAGAAAGAAGTTCTGCAACGAAGCGATCGGCTCACACGCGGATACACAGATGGGTTTGTACCGAGAA
    CGTTAGGTTTGTGAACCGAGTTCAGGTAAAACAAAGTAGATTGTGTGCCTTTACGCAGACAGCGAGGGAA
    AACATAG
  • Protein Sequence : Show Sequence
    >CAB56644.1 MIC3 microneme protein [Toxoplasma gondii]
    MRGGTSALLHALTFSGAVWMCTPAEALPIQKSVQLGSFDKVVPSREVVSESLAPSFAVTETHSSVQSPSK
    QETQLCAISSEGKPCRNRQLHTDNGYFIGASCPKSACCSKTMCGPGGCGEFCSSNWIFCSSSLIYHPDKS
    YGGDCSCEKQGHRCDKNAECVENLDAGGGVHCKCKDGFVGTGLTCSEDPCSKRGNAKCGPNGTCIVVDSV
    SYTCTCGDGETLVNLPEGGQGCKRTGCHAFRENCSPGRCIDDASHENGYTCECPTGYSREVTSKAEESCV
    EGVEVTLAEKCEKEFGISASSCKCDNGYSGSASATSHHGKGESGSEGSLSEKMNIVFKCPSGYHPRYHAH
    TVTCEKIKQ
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): T. gondii DNA vaccine pMIC3i encoding the complete MIC3-ORF (RH(ERP) strain)
13. MIC8
  • Gene Name : MIC8
  • NCBI Nucleotide GI : 13346486
  • NCBI Protein GI : 13346487
  • Protein Accession : AAK19757.1
  • Taxonomy ID : 5811
  • Gene Strand (Orientation) : ?
  • Protein Name : microneme protein 8
  • Protein pI : 5.13
  • Protein Weight : 71329.71
  • Protein Length : 747
  • DNA Sequence : Show Sequence
    >gi|13346486|gb|AF353165.1| Toxoplasma gondii microneme protein 8 (MIC8) gene, complete cds
    ATGAAGGCCAATCGAATATGGTGTTTTTTTGCGTGGCGTATGGTTGTGCGGGCCTCATTTCTGAAAGAGA
    TGGACAGCATTTTCGTTTCTGCTATCCGACAGAATGTACAGCATACTCATTCTGCCCTTCTCGCCAAACT
    GAAGGAACCCCCAGATCCAGATGATGAGAACTCTTGGCTTTGTCGAATATCAAAAAAATATGACGCATGC
    GGTAGCAGGGAATATTCCGATAAGGGCCTCAAAGGGACGTACTGTCCCGAGGATTTTTGCTGTAGCAAGA
    CGGCATGTTTTTACGGTTCATGTGGGAGTTGGTGCCACGACAACTGGGCTCTGTGCAGCTCATCTATAAT
    CTACCACGACGAGTACAGTTACGGGAAATGCAACTGTAAACGGTTTCAAGAAAACTGTGATGTGAATGCA
    ATTTGTGTGCATGCGAACAGAGAGGATGGCGGTGCGTATTGTCAGTGCAAGGAAGGATATTGGGGTGATG
    GTAAATCGTGCAAGATTGACTTCTGCCAACTGCAGCCCTGTGGTGCAGGGACCTGCACCAGGACGGATGA
    AGGATACAAGTGTGATTGCCCAGAAACTCACAAGCTTATTGTCGTTGAAGACAAAGAGACGTGCAAGGCA
    AAACCGGACTTTTGCGCGGAAGAGCCTTGCGGACCACCCTCTATGGTTGAAAATTGCGTGAACACCGATG
    ACAGCTACGAATGTGTTTGCAAACAGGGGTATGAAGTGAGGAACGGTCGGTGCGAAGAAATTGACTTATG
    CGCGGACAAGCCATGTGGGCCAGATGAGGGTGTGCATGAGTGTGTAACAGAGAGGCAACCGAAATTAAGG
    TACAGATGCACGTGCAAGGCAGGATTCGATTTGACCACCTTGCCTGATGGTGTTTCCCAGAAGTGCCTGA
    AGAACTTCTGTTACGAGGAGCCCTGCGGCACCCGGGACCTAGTTGAAAGCTGTAAGTCAAAGGCATATGG
    ATACTCGTGTTTGTGTGCGGCAGGTGCCATGGTTCAAGTGATTAACGGAAAAGAAAAGTGCATCAAGGCG
    GACTTGTGCCGCAATGATCCGTGTGGTCCAGAGACAGCAGTGATTCAATGTTACTCTCATGGCACCAGCT
    ATAGGTGTTTGTGCAAAGCAGGCTACACTGAAGTTTTTGTTAACGGGAAGAGTTCATGTCAAAAGGGCGA
    CCCATGCACTCTGAACATGTGTGGCGGTAACGAAGCGGTCCAGGAGTGCACAACTGATGGCACGGCGTAC
    GGGTGTACCTGCAAGCCAGGCTATTCGATAGCCATTAAGCATGGTCAGAAGTTTTGCAACCCTGAAGAGG
    AGTGTGCTTCTCATTGTGGCTCGGCAGCTGCAGTGAAAAGCTGTGAAATACTTGACTCTGGCGGATACCA
    GTGTACATGCAATCCAGGATACGTCATGAGATACAGCGACTATGTAAAAGGATGCGTCGAGGGAAATCAG
    TGTTCTCTCAATCCTTGTGGGGAGCAGGAAGCCGTGCAAAGGTGCATTCCTGAAGGTGACACGTATGATT
    GCGAGTGCAATCCGGGGTTCGTCAAAAGAGTCTTGCCGGATGGGAATTTCATTTGCGCCGATCCAGCGAG
    CTGTGTAGGGAATCCCTGTGGTAGCTCAGATGCGGTCGATGCGTGCATTGCCGGGACTAGCACGTATACA
    TGCAGGTGTAAGGACGGATACACACCTCAGTCAATTGGGTCAAAGTTGCAGTGTTTACCAGAAAGCACTG
    ATCAGACAGATTTCGATTCCAAACACAAACCAGAGGACAACAAAGGTCGATATTCGAAAGGAACAATTGC
    ATTGGTGGTTGTGGGGTGTGTAGCCTTGTTGGGTATTATAGCCGGAGGAATTTCTTACGCCAGAAACAGA
    GGAGGTGAGCGCGATGATGAAGACTTGGCACCACCACCTCGTTCCACACGAGAACGGAGGCTCTCATCAA
    TGGGCGAAGGTTTTGAGAATGCCTCATGGGCATCTTCTGTAAGTATGATTCCTAGTGCACCTGCTCCGCC
    ACCTTCGGGCGGTATCTGGTCCTAA
  • Protein Sequence : Show Sequence
    >AAK19757.1 microneme protein 8 [Toxoplasma gondii]
    MKANRIWCFFAWRMVVRASFLKEMDSIFVSAIRQNVQHTHSALLAKLKEPPDPDDENSWLCRISKKYDAC
    GSREYSDKGLKGTYCPEDFCCSKTACFYGSCGSWCHDNWALCSSSIIYHDEYSYGKCNCKRFQENCDVNA
    ICVHANREDGGAYCQCKEGYWGDGKSCKIDFCQLQPCGAGTCTRTDEGYKCDCPETHKLIVVEDKETCKA
    KPDFCAEEPCGPPSMVENCVNTDDSYECVCKQGYEVRNGRCEEIDLCADKPCGPDEGVHECVTERQPKLR
    YRCTCKAGFDLTTLPDGVSQKCLKNFCYEEPCGTRDLVESCKSKAYGYSCLCAAGAMVQVINGKEKCIKA
    DLCRNDPCGPETAVIQCYSHGTSYRCLCKAGYTEVFVNGKSSCQKGDPCTLNMCGGNEAVQECTTDGTAY
    GCTCKPGYSIAIKHGQKFCNPEEECASHCGSAAAVKSCEILDSGGYQCTCNPGYVMRYSDYVKGCVEGNQ
    CSLNPCGEQEAVQRCIPEGDTYDCECNPGFVKRVLPDGNFICADPASCVGNPCGSSDAVDACIAGTSTYT
    CRCKDGYTPQSIGSKLQCLPESTDQTDFDSKHKPEDNKGRYSKGTIALVVVGCVALLGIIAGGISYARNR
    GGERDDEDLAPPPRSTRERRLSSMGEGFENASWASSVSMIPSAPAPPPSGGIWS
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : The gene sequence encoding MIC8 was inserted into the eukaryotic expression vector pVAX I, and the pVAX-MIC8 expression plasmid was constructed, and the plasmid diluted with PBS to 100 mg/100 microl was injected into the Kunming mice muscularly. Levels of IgG antibody, gamma-interferon (IFN-gamma), interleukin-2 (IL-2), interleukin-4, and interleukin-10 were detected. The mice were challenged with tachyzoites of the virulent T. gondii RH strain at the 14th day after the last immunization to observe the survival time. The high level of IFN-gamma, IL-2, and IgG antibody indicated that mice vaccinated with recombinant pVAX-MIC8 plasmid could elicit strong cellular and humoral immune responses and showed a significantly increased survival time (10.3 +/- 0.9 days) compared with control mice which died within 5 days of challenge infection (Liu et al., 2010).
  • Related Vaccine(s): T. gondii DNA vaccine encoding MIC8
14. OMPDC
  • Gene Name : OMPDC
  • Sequence Strain (Species/Organism) : Toxoplasma gondii ME49
  • NCBI Gene ID : 7899615
  • NCBI Protein GI : 237831731
  • Locus Tag : TGME49_059690
  • Genbank Accession : DS984728
  • Protein Accession : XP_002365163
  • Taxonomy ID : 508771
  • Gene Starting Position : 2298541
  • Gene Ending Position : 2301456
  • Gene Strand (Orientation) : +
  • Protein pI : 4.79
  • Protein Weight : 31611.79
  • Protein Length : 320
  • DNA Sequence : Show Sequence
    >gi|212659390:2298541-2301456 Toxoplasma gondii ME49 scf_1112359874900, whole genome shotgun sequence
    GATGCCGGCAGACGCGCTGCGTGAGAAGCCGGTTGTCCAGGCTGCTGTGAATACCTCGGAAGGGTGCAAG
    AACTTCTTCAAGACATTGGATGCAAGGATCGAAGCGGTAGATTCCTTGCTCACAGTCGGCCTTGATCCGC
    ACATCGCGGACCTCCCGTCGCCAGCCACAGCTGAGCGTGCCTTTGTATTTTGTGAACGCATCATCAGGGA
    AACTCTTCCTTTTACGTGTTGTTACAAACCGAACAGCGCGTTTTTTGAGGCATTTGGTCCCCCGGGTTTG
    CAGGTACGTATTTCAATGTGAGGATGGAGCATGGTTGTCATGGCCAGATATACTTTAGCCCGACAACGTT
    ACCAGGCATTTGGAGTACCTCCAACTCCTCAGTTGCAAAGATACACTGCACATGCTCGTGTAGTACCGCT
    GAGAGACAGAACCACTTGAAACGTCATCTTTCATTAGTATGAAGCGGTTGTCCGAAGTTCGGCCATCATT
    CTGGAATCTATCAGTCCTATGTTTCCTCACTGCCTAGGTACTGTTTTCCTGTTTGTGTATATCCCGAAAA
    ACTGTATTGAAAGCAAGCAGTGGGGATAGCGGTGAAGCAGGGGGTTGGTGAAGCGGTTTCGGAGGGGACA
    GGGAAATACCTTCAATTTTTGAAAGGCGCAGTTCCCTGTTCCTTCGCAGTCACCACGGACAGACTAGAGA
    TATGTCTGTTCTGTACGCGCCTTACCAAGACCCGAACCGCGTACAGGGTGCAAACCTTTCGCGCCATAAT
    CGAATGCCGCTTGTCGCCACGTGTAGGCACTCGAGAGAGTCTGCGCGCTGATTCCCGACGATGTTCCTAT
    TCTTCTAGACGCCAAAAGAGGGGACATAGGATCGACGGCGCAAGCCTACGCATCTTCGGCTTTTGACGCG
    TTCAAGGTATGGAACTTCGAGCCCTAAGAGGTTGAAGAGAGTACGTAGAACCATGTTTATTTCATTAACA
    TCGAGTCGGTTCTGTATACATACGCGTGCTGCTGCAGTGAATGTATTACAAGATATCTCGTATGCATGCG
    CCCGACGAGAATATCGCCAATATCATAGCCTTATGACGTGCGCGATTCGTCTGGTGGCAAATCTGCGGTC
    TACAAGTGATCTATTGTCAGCGCATGGCAGTAACGATCTCATCCATTTATGCGGGTGACGCATTACATCG
    TGTCTTTTGTCAAAAATGTGCTGCTTGATGTCGCGCAGGCGGACGGTGTCACCGTCAACGCCTACATGGG
    CAGAGAAGCAGTTCGACCGTTTCTGTCGTATCGAAACAAAGGCGTATTTGTACTTGTGAAGACGTCGAAT
    CAATCGTCCAACGAGTTTCAGACCCTGCCAGTGAGGGTGGAGTCAAGCTCCGACACTCAGGATGTCCCGC
    TGTATGTACAGATGGCCAGAGTTTGCAACGAACTTGCCCAAGGTGAGAGTGGGTCCGTCAGCTTCGTTAG
    ACCAGCTTCTCTTCCGTTGTATTTGTCTTTTGTGAGCGACCCGTGTCGCCAGAGCTTCATGCTTAGCTAC
    ACCGCTCGGCAGAACTGGCATCTCTCTCGCTCCAGGAGTGTGATCCGCGTTTCCAGTTTTTGGTTGATAA
    GTCGCACCCCAGAGGAGAAAAATCCTCAGCACCTGCCATACAGATTCAACGGAGTCTCAGGCAGCTTTTT
    ACTCCGAGCCCGCACTTGCCCGACCTGCCATTTTCGTTTACACAGAGCCACTTTCTGCGGGGCGGTGGCG
    ACAAAAACCGCGAGCTTTGTAGTAAATGAAACCACGTCGTGAGAAGTAGTCCAGTTCTCCCTTCGAACAC
    TACGTTGCCTGCTACATATCTGTGCCTCATCGTCGAGTGCCGCTGTCCGTGTCTTGCTCCACTTTGTCAA
    TCCGAACTCAGAATGCGTTTCTGAGGAAGACTCTCCATCCACGACTGGACATGGCCTCCGTCATAATGCG
    TCGGGCGGTGGTGTCGGACTCGTAGTAGGAGCAACAGATATAGAAGCTCTTCGCGAGGTGCGAAAGGCAT
    GCCCGGACCTTTATATTCTTGCTCCCGGAGTCGGTGCCCAAGGAGCGGACCTCGAGAGGGCCTTGAGTGC
    CGGTAAGCATGGAGCTGGGTCTCAGTTGAGAGCGAAGTTACACAATTGAATCTGACATTGAGGTTGATTT
    GATTGTCGCTTGAGGCGCCTGTGACCCTAATGATCCCGAAACGCAGGAACCATAGAAGCAACGGAGGGAG
    ACGGAACCTTGCTGTTTTAGGTCATTGTGCGCTAAACGTGGTTTCTGGTACACCGTGCAAGATCGTATAC
    TCACGGGTATGATCGCAGTGAAAATGCTCCTAAGAGCGAAGAAATAAGGTACCACATTTTCAACACCTCT
    ACGGTTTTTCCTGTTACTTGGGACTCGTTGCTCTGCCAGGTCTCTGCAAGGACGGCAAAGGGATGCTTAT
    TCCCGTCTCTCGTGGCATATCACGAGCGGAGGTAAGGGCAGGTTGTCTTGACACTTGTAGCCCGTAGTTT
    TTGAAAATCCAGCACACCGCATAGAACCTTGGTGACAGAGATGCGTGCGTCACTCGAACGAATATTCCGA
    TGACGGCGAAGTTGACTGAAGTATTTCTGTTTTCAGCAGTATATTTTTCAGCGTTTGTATGGCCCCAGGA
    TGGATGCAAAACCAACCGACTGCGCCCTTCGCTCAGATGTGCGGAATCCACCGGATCCATGCAATAGAAA
    ATAGATTTCAGATGACACGGATTTGAGATGAAATCACCCGTTCATAGTACAGCTGTTTGCGTCCACTTTG
    TCCGTCCTCCCTGTTCCGTTCCCTTCATTGCAGAATTTAGCTGCCCAAGCTAACGCATACAGAGAACAGA
    TCAATGAAGTCAGAAGAGGCATTGTCCAGCAGTACGAGGAAAGCTG
  • Protein Sequence : Show Sequence
    >gi|237831731|ref|XP_002365163.1| orotidine-monophosphate-decarboxylase, putative [Toxoplasma gondii ME49]
    MPADALREKPVVQAAVNTSEGCKNFFKTLDARIEAVDSLLTVGLDPHIADLPSPATAERAFVFCERIIRE
    TLPFTCCYKPNSAFFEAFGPPGLQALERVCALIPDDVPILLDAKRGDIGSTAQAYASSAFDAFKADGVTV
    NAYMGREAVRPFLSYRNKGVFVLVKTSNQSSNEFQTLPVRVESSSDTQDVPLYVQMARVCNELAQECVSE
    EDSPSTTGHGLRHNASGGGVGLVVGATDIEALREVRKACPDLYILAPGVGAQGADLERALSAGLCKDGKG
    MLIPVSRGISRAENLAAQANAYREQINEVRRGIVQQYEES
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An OMPDC mutant, alone or in combination with an UP mutation, is attenuated in mice and induces significant protection from challenge with wild type T. gondii (Fox and Bzik, 2010).
  • Related Vaccine(s): Toxoplasma gondii OMPDC mutant vaccine , Toxoplasma gondii OMPDC/UP mutant vaccine
15. p30
  • Gene Name : p30
  • Sequence Strain (Species/Organism) : Toxoplasma gondii
  • NCBI Nucleotide GI : 894214
  • NCBI Protein GI : 894215
  • Protein Accession : AAB33440.1
  • Other Database IDs : CDD:332018
  • Taxonomy ID : 5811
  • Gene Strand (Orientation) : ?
  • Protein Name : p30 antigen
  • Protein pI : 8.08
  • Protein Weight : 32194.82
  • Protein Length : 386
  • Protein Note : SRS domain; cl27197
  • DNA Sequence : Show Sequence
    >gi|894214|gb|S76248.1| p30=p30 antigen [Toxoplasma gondii, RH, Genomic, 1634 nt]
    CGCGTGTTCTAACCACAAACCTTGAGACGCGTGTTCCAACCACGCACCCTGACACGCGTGTTCCAACCAC
    GCACCCTGAGACGCGTGTTCTAACCACGCACCCTGAGACGCGTGTTCTAACCACGCACCCTGAGACGCGT
    GTTCTGCCGCACAATGTGCACCTGTAGGAAGCTGTAGTCACTGCTGATTCTCACTGTTCTCGGCAAGGGC
    CGACGACCGGAGTACAGTTTTTGTGGGCAGAGCCGTTGTGCAGCTTTCCGTTCTTCTCGGTTGTGTCACA
    TGTGTCATTGTCGTGTAAACACACGGTTGTATGTCGGTTTCGCTGCACCACTTCATTATTTCTTCTGGTT
    TTTTGACGAGTATGTTTCCGAAGGCAGTGAGACGCGCCGTCACGGCAGGGGTGTTTGCCGCGCCCACACT
    GATGTCGTTCTTGCGATGTGGCGTTATGGCATCGGATCCCCCTCTTGTTGCCAATCAAGTTGTCACCTGC
    CCAGATAAAAAATCGACAGCCGCGGTCATTCTCACACCGACGGAGAACCACTTCACTCTCAAGTGCCCTA
    AAACAGCGCTCACAGAGCCTCCCACTCTTGCGTACTCACCCAACAGGCAAATCTGCCCAGCGGGTACTAC
    AAGTAGCTGTACATCAAAGGCTGTAACATTGAGCTCCTTGATTCCTGAAGCAGAAGATAGCTGGTGGACG
    GGGGATTCTGCTAGTCTCGACACGGCAGGCATCAAACTCACAGTTCCAATCGAGAAGTTCCCCGTGACAA
    CGCAGACGTTTGTGGTCGGTTGCATCAAGGGAGACGACGCACAGAGTTGTATGGTCACGGTGACAGTACA
    AGCCAGAGCCTCATCGGTCGTCAATAATGTCGCAAGGTGCTCCTACGGTGCAGACAGCACTCTTGGTCCT
    GTCAAGTTGTCTGCGGAAGGACCCACTACAATGACCCTCGTGTGCGGGAAAGATGGAGTCAAAGTTCCTC
    AAGACAACAATCAGTACTGTTCCGGGACGACGCTGACTGGTTGCAACGAGAAATCGTTCAAAGATATTTT
    GCCAAAATTAACTGAGAACCCGTGGCAGGGTAACGCTTCGAGTGATAAGGGTGCCACGCTAACGATCAAG
    AAGGAAGCATTTCCAGCCGAGTCAAAAAGCGTCATTATTGGATGCACAGGGGGATCGCCTGAGAAGCATC
    ACTGTACCGTGAAACTGGAGTTTGCCGGGGCTGCAGGGTCAGCAAAATCGGCTGCGGGAACAGCCAGTCA
    CGTTTCCATTTTTGCCATGGTGATCGGACTTATTGGCTCTATCGCAGCTTGTGTCGCGTGAGTGATCACC
    GTTGTGCTCACTTCTCAAATCGACAAAGGAAACACACTTCGTGCAGCATGTGCCCCATTATAAAGAAACT
    GAGTTGTTCCGCTGTGGCTTGCAGGTGTCACATCCACAAAAACCGGCCGACTCTAAATAGGAGTGTTTCG
    CAGCAAGCAGCGAAAGTTTATGACTGGGTCCGAATCTCTGAACGGATGTGTGGCGGACCTGGCTGATGTT
    GATCGCCGTCGACACACGCGCCACATGGGTCAATACACAAGACAGCTATCAGTTGTTTTAGTCGAACCGG
    TTAACACAATTCTTGCCCCCCCGA
  • Protein Sequence : Show Sequence
    >AAB33440.1 p30 antigen [Toxoplasma gondii]
    MSVSLHHFIISSGFLTSMFPKAVRRAVTAGVFAAPTLMSFLRCGVMASDPPLVANQVVTCPDKKSTAAVI
    LTPTENHFTLKCPKTALTEPPTLAYSPNRQICPAGTTSSCTSKAVTLSSLIPEAEDSWWTGDSASLDTAG
    IKLTVPIEKFPVTTQTFVVGCIKGDDAQSCMVTVTVQARASSVVNNVARCSYGADSTLGPVKLSAEGPTT
    MTLVCGKDGVKVPQDNNQYCSGTTLTGCNEKSFKDILPKLTENPWQGNASSDKGATLTIKKEAFPAESKS
    VIIGCTGGSPEKHHCTVKLEFAGAAGSAKSAAGTASHVSIFAMVIGLIGSIAACVA
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): T. gondii DNA vaccine pcDNA3.1-SAG1-ROP2 , T. gondii DNA vaccine pSAG1-ROP2-SAG2
16. PI1
  • Gene Name : PI1
  • Sequence Strain (Species/Organism) : Toxoplasma gondii
  • NCBI Protein GI : ADJ17341
  • Other Database IDs : CDD:238052
  • Taxonomy ID : 5811
  • Protein Name : serine protease inhibitor 1 isoform beta
  • Protein pI : 4.61
  • Protein Weight : 30858.24
  • Protein Length : 373
  • Protein Note : Kazal type serine protease inhibitors and follistatin-like domains. Kazal inhibitors inhibit serine proteases, such as, trypsin, chyomotrypsin, avian ovomucoids, and elastases. The inhibitory domain has one reactive site peptide bond, which serves the...; cd00104
  • Protein Sequence : Show Sequence
    >ADJ17341.1 serine protease inhibitor 1 isoform beta [Toxoplasma gondii]
    MGKNPLLFLAGVLVCSFSVAVFASPETKVCSCPRNLELNCGGDHVTYANHCIRECHGVGLLHDGPCADQE
    DQGSSVNEPSEVADTTEQPSITPSGEEDRPPKDAPKERKRLRGCACTRELRLNCGVDGVTYSNHCVRKCE
    RVKLLHEGPCRGGPGRTLFSQQDDESENDALEEEEQRLQSCPCPLNLMLNCGVDGVTYDNHCLRRCRRVE
    LKHEGPCEADEQKQLPVQHGDNEIDVPERRIEDLKDCACPLILTPNCGTDRKTYPNNCVRECAGVKLLHE
    GPCEGDTEEIWDDQ
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Picchio et al., 2018)
17. Rop2
  • Gene Name : Rop2
  • Sequence Strain (Species/Organism) : Toxoplasma gondii
  • VO ID : VO_0011340
  • NCBI Protein GI : 115299638
  • 3D structure: PDB ID : 2W1Z
  • Other Database IDs : CDD:291215
    CDD:304357
  • Taxonomy ID : 5811
  • Gene Strand (Orientation) : ?
  • Protein Name : Rop2 protein
  • Protein pI : 9.64
  • Protein Weight : 28699.38
  • Protein Length : 316
  • Protein Note : Kinase-like; pfam14531
  • Protein Sequence : Show Sequence
    >ABI93795.1 Rop2 protein, partial [Toxoplasma gondii]
    LRLLRGIKNQKQAKVHLRFIFPFDLVKDPKKRKMIRVRLDERDMWVLSRFFLYPRMQSNLQMLGDVLLSH
    SSTHKSLVHHARLQLTLQLIRLLASLHHYGLVHADFQVSNILLDQRGGVFLTGFEHLVRDGASAVSPIGR
    GFAPPETTAERMLPFGQHHPTLMTFAFDTWTLGLAIYWIWCADLPNTDDAALGGSEWIFRSCKNIPQPVR
    ALLEGFLRYPKEDRLLPLQAMETPEYEQLRTELSAVLPLYQTDGEPA
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : A study to examine the immunostimulatory properties of Leishmania infantum Hsp83 (83) to elicit humoral and cellular response against the Toxoplasma gondii Rop2 protein in an adjuvant-free vaccination system was conducted. Mice immunized with fusion Rop2-83 elicited a stronger humoral and cellular response in comparison to mice immunized with Rop2 alone, or a mix of LiHsp83 and Rop2. The fusion protein induced a Th1 type response, with predominance of specific IgG2a/IgG2c isotype and IFN-gamma secretions, whereas Rop2 alone or mixed with LiHsp83 produced a Th1/Th2 mixed response. Vaccination with fusion protein conferred a remarkable resistance against oral infection with ME49 cysts in C57BL/6 and C3H mice, in comparison to mice immunized with Rop2 alone or the protein mixture. Following lethal challenge, a significant survival rate was observed in Rop2-83 immunized Balb/c and C57BL/6 mice in comparison to control groups (Echeverria et al., 2006).
  • Related Vaccine(s): T. gondii DNA vaccine pcDNA3.1-SAG1-ROP2 , T. gondii Subunit Rop2-Hsp83 Fusion Protein Vaccine
18. ROP2
  • Gene Name : ROP2
  • Sequence Strain (Species/Organism) : Toxoplasma gondii RH
  • NCBI Protein GI : 563627
  • Other Database IDs : CDD:291215
    CDD:287472
    CDD:304357
    GOA:Q27007
    InterPro: IPR000719
    InterPro: IPR011009
    InterPro: IPR016815
    PDB: 2W1Z
    UniProtKB/TrEMBL: Q27007
  • Taxonomy ID : 5811
  • Gene Strand (Orientation) : ?
  • Protein Name : ROP 2
  • Protein pI : 8.19
  • Protein Weight : 61058.83
  • Protein Length : 609
  • Protein Note : Kinase-like; pfam14531
  • Protein Sequence : Show Sequence
    >CAA85377.1 ROP 2 [Toxoplasma gondii]
    MENCASVRSSSCLIWLAAAFFVSALGHVQQGAGVVRPRHWQNSEAAVSVRPPGGASPRHFHSPIEPVAFI
    DGEHVEDKHGGSWLEQEAAEEVTPLLNSHTETPTQSPSAFRRLLRRLRFWRRGRTGGSDGGGEPPQTPRP
    SLPTRLFQHLRRAAAAIPAAASRFFRRFRRVQEPVFPPDEFPEDVDTNPMYFRGTDPGDVVIEELFNRIP
    ETSVWNENERVLSNANHLVSTALWRNEQSFRVESELGERPRTLVRGPVLRDDGSYICLEATDQETGEPLE
    VHVPYFTERPPSNAIKQLSEQVLRLRLLRGIKNQRQAKAYLRFIFPIDLVKDPKKRKMIRVRLDERDMWV
    LSRFFLYPRMQSNLHILGDVLLSHSSTHKSLVHHARLQLTLQLIRLAASLQHYGLVHADFQVRNILLDQR
    GGVFLTGFEHLVRDGASAVSPIGRGFAPPETTAERMLPYRQHHPTLMTFPFDTWTLGLAIYWIWCADLPN
    TEDAELGGIEWIYRRCKNIPQPVRALLEGFLRYSKEDRLLPLQAMETSEYEQLRTELSAVLPLYQTDGEP
    A
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): T. gondii DNA vaccine pSAG1-ROP2-SAG2
19. Rop2
  • Gene Name : Rop2
  • Sequence Strain (Species/Organism) : Toxoplasma gondii
  • NCBI Protein GI : ABI93795
  • Other Database IDs : CDD:291215
    CDD:304357
  • Taxonomy ID : 5811
  • Protein Name : Rop2 protein
  • Protein pI : 9.64
  • Protein Weight : 28699.38
  • Protein Length : 316
  • Protein Note : Kinase-like; pfam14531
  • Protein Sequence : Show Sequence
    >ABI93795.1 Rop2 protein, partial [Toxoplasma gondii]
    LRLLRGIKNQKQAKVHLRFIFPFDLVKDPKKRKMIRVRLDERDMWVLSRFFLYPRMQSNLQMLGDVLLSH
    SSTHKSLVHHARLQLTLQLIRLLASLHHYGLVHADFQVSNILLDQRGGVFLTGFEHLVRDGASAVSPIGR
    GFAPPETTAERMLPFGQHHPTLMTFAFDTWTLGLAIYWIWCADLPNTDDAALGGSEWIFRSCKNIPQPVR
    ALLEGFLRYPKEDRLLPLQAMETPEYEQLRTELSAVLPLYQTDGEPA
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Picchio et al., 2018)
20. ROP8
  • Gene Name : ROP8
  • Sequence Strain (Species/Organism) : Toxoplasma gondii strain RH (EP)
  • NCBI Nucleotide GI : 2293518
  • NCBI Protein GI : 2293519
  • Protein Accession : AAC47797.1
  • Other Database IDs : CDD:291215
    CDD:304357
  • Taxonomy ID : 5811
  • Gene Strand (Orientation) : ?
  • Protein Name : ROP8
  • Protein pI : 9.42
  • Protein Weight : 61810.69
  • Protein Length : 622
  • Protein Note : Kinase-like; pfam14531
  • DNA Sequence : Show Sequence
    >gi|2293518|gb|AF011377.1| Toxoplasma gondii rhoptry antigen ROP8 gene, complete cds
    GAATTCGCTAGTCTCAGGTCGAGTCGCCGGGGCGTCAGGAGACAAGCACCGGCGCCCCGCGCGCCAACTG
    TCCGTCCTTCCGGGTGAGTTGACGTTTTGAGCCACGCTGCAGAACTCTGCACTGGAGTGATTGAGCGCGG
    CATTTATGTCAATCTGGTTTTTCCACGTGTATTCCAGAAGCTTGCCCTGAGAAATACAGATACACTGTCA
    CTTTTGAATCGATGTTTTCTGTGTTACGTAACCGCAACATGAGACACGGTCCCTCGATTGGATCGTCATC
    ATGTCTTATCTGGCTAGCTGCCGCATTCTTTGTTTCGGCACTTGGCCACGTACAGCAAGGCGCTGGCGTT
    GTGCGGCCTCGCCACTGGCAGAACTCGGAAGCCGCTGTTAGTGTCCGGTCGCCGGGAGGCGCGTCCCCTA
    GACATTTCCACAGCCCAATTGAGCCAGTAGCATTTATTGATGGGGAGCACGATGAAGACAAGCATGAAGG
    CTCATGGTTGGAGCAGGAAGCGGCCGAGGATGTGACCCCTTTACCGGGCAGCCACACAGAGGCCCAGACA
    CAGTCCCCCAGTGCTTTTAGAAGGTTAACCAGGCGTTTGCGTTTTTGGCGACGTGGGGTGACAGGCGGAT
    CAGATGCTGGAGAAGAGGCGCCTCAGACGCCTCGACCTCCACTACGTACACGAATCCTTCAGTACTTGCG
    GCGTGTGGGAACATTTTTCAGACGAGACATTCCAGCGGCAGCACTTCGATTCTTCAGGCGCTTTCGACGA
    GTCCGCCAACCTGTATTCCCTCCCGACGAGTTTCCGGAGGATGTCGACACGAACCCTATTTATTTCCGTG
    GTACGGATCCTGGAGACGTTGTCATTGAGGAGCTGTTCAATCGTATACCGCAAGCAAACGTACGGACTAC
    AAGTGATTACATGCAGTCCGCAGCTGACAGTCTAGTGTCCACATCGTTGTGGAATATTGGACAGCCCTTC
    CGCGTAGAGTCGGAGCTTGGCGAGCGTCCAAGGACGCTAGTCAGGGGCACATTGCTCGGCCAGGAAGACC
    CTTATGCCTATGTCGAGGCGACCGACCAGGAGACAGGAGAATCATTTGAGGTGCACGTTCCACTTTCCAA
    GGAAAAGCCGTCTTCCAACACGATCAAGCAGATGAAGCAGGAGGTGCTGCGCCTACGCTTGCTACGAGGC
    ATCAAAAACCAGAAGCAAGCCAAGGTGCATCTCAGATTTATATTCCCCTTCGATTTGGTGAAGGACCCAC
    AGAAAAAGAAGATGATCCAGGGTGTCTCAGATGAGGGGGCTGGCTGGATCGTGAACGGATTCTTTCTGTA
    TCCCCGAATGCAGACTAACCTTCAGACGCTTGGAGAAGTCCTACTGAGTCATTCCTCAACACACAAGTCC
    CTCGTGCACCACGCTCGGTTGCAGCTCACGCTTCAGGTCATAAGGCTGCTTGCGGGTCTCCACCACTATG
    GCCTTGTGCATACCTTTTTTAGGGCCGTCGATATCGTCTTGGACCAGCGTGGTGGCGTGTTTTTGACCGG
    CTTTGAACATCTGGTGCGAGACGGCGACAGGGTGGTGTCGACCGTCTGTCGAGGATTCGAACCGCCGGAG
    CTTGAAGCCAGACGAGCAACCATATCTTACCACAGGGACCGCAGGACGCTGATGACATTTTCGTTTGATG
    CATGGGCATTGGGGTTGGTGATCTACTGGATTTGGTGCGCCGATTTGCCCAATACCAAGGACGCGCCGCT
    AGGCGGAAGTGACTGGATCTTTCGACGCTGCAAGAATATCCCACAGCCAGTCAGAGCTTTGCTTGCGGGA
    TTCTTGCGATACCCGCAAGAGAATCGGCTCCTSCCATTGCAAGCCATGGAGACTCCTGAGTACGAGCAAC
    TGCGCACAGAGCTATCAGCCGTTTTGCCCCTGTATCTTTCTGATGGAGAACCGGCATGAGAGGGTGGCGC
    GCCACCATCGGGAACATCTCAGCCGGACGAAGCTGGAGTCGCTGAGGCGGTTACGGCAATCTAGAACCTC
    GAGGAGGGGCCAGCGATGAGCTTGAATTC
  • Protein Sequence : Show Sequence
    >AAC47797.1 ROP8 [Toxoplasma gondii]
    MFSVLRNRNMRHGPSIGSSSCLIWLAAAFFVSALGHVQQGAGVVRPRHWQNSEAAVSVRSPGGASPRHFH
    SPIEPVAFIDGEHDEDKHEGSWLEQEAAEDVTPLPGSHTEAQTQSPSAFRRLTRRLRFWRRGVTGGSDAG
    EEAPQTPRPPLRTRILQYLRRVGTFFRRDIPAAALRFFRRFRRVRQPVFPPDEFPEDVDTNPIYFRGTDP
    GDVVIEELFNRIPQANVRTTSDYMQSAADSLVSTSLWNIGQPFRVESELGERPRTLVRGTLLGQEDPYAY
    VEATDQETGESFEVHVPLSKEKPSSNTIKQMKQEVLRLRLLRGIKNQKQAKVHLRFIFPFDLVKDPQKKK
    MIQGVSDEGAGWIVNGFFLYPRMQTNLQTLGEVLLSHSSTHKSLVHHARLQLTLQVIRLLAGLHHYGLVH
    TFFRAVDIVLDQRGGVFLTGFEHLVRDGDRVVSTVCRGFEPPELEARRATISYHRDRRTLMTFSFDAWAL
    GLVIYWIWCADLPNTKDAPLGGSDWIFRRCKNIPQPVRALLAGFLRYPQENRLLPLQAMETPEYEQLRTE
    LSAVLPLYLSDGEPA
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): T. gondii DNA vaccine ROP8-pVAX-1
21. SAG1
  • Gene Name : SAG1
  • Sequence Strain (Species/Organism) : Toxoplasma gondii ME49
  • VO ID : VO_0011000
  • NCBI Gene ID : 7900172
  • NCBI Protein GI : 237839159
  • Locus Tag : TGME49_234370
  • Genbank Accession : KE138836
  • Protein Accession : XP_002368877
  • 3D structure: PDB ID : 1KZQ
  • Taxonomy ID : 508771
  • Chromosome No : X
  • Gene Starting Position : 4326607
  • Gene Ending Position : 4329557
  • Gene Strand (Orientation) : -
  • Protein Name : surface antigen SAG1
  • Protein pI : 7.13
  • Protein Weight : 18124
  • Protein Length : 174
  • DNA Sequence : Show Sequence
    >NC_031478.1:4326607-4329557 Toxoplasma gondii ME49 chromosome X, whole genome shotgun sequence
    AGACTGGCATTGCGACCCGACCAGTAGCAAGCTCTGCTCGAGAGGCAATTTGTAGAGATGTTTTGAGCAG
    TTTGATGGTGTGTGAGAAGAGGACACGGTTGGGGTCCTCTGTCATTCGAGAACACGATCCAGCCCTTAGA
    AAGCATGACTTATGTCGGCGGCGCCCCCTTTCCTCGGGTACCTGTTTTGTCCCCAGGATGAGCAAGCACC
    GCTTGTTACACTCGGCAGATTTGAAGTGGGCAGTCACGAGAAAACTGATGTGGCCAGTACGCTCCACCAT
    GTTACTGCACTCTCATCACCCGCGATTCTCTGCAAGAACAGATTCAGAGGGTCCCACAATTCCCTATTCC
    ACGACACACTCTCGCAGCAGTGCCACAAGCACAAAAGGGTTTGTTTCGACGCATGAAATAGACCGCCCCT
    CCAGAGAAAACCTGTATTGGTGCAGCCATTCAAAGCATAGCGGGGCATGATCAACGACTGCCTGCTGCAT
    TTTCGGAGTTGGGGCTCTTCAAAACAAACGACGTGAGATGGCTTTGCCACAAGATAGTGCTAAATGCCTG
    TTTTTCCATACGAGGTTCGTTACACCGACTTCGGTGTCGCCCTGCAAAATGCTGACCGCTTCCCGCATTG
    TCTATGATCCTGCCAGAATGGAGTCACCCTCATGCCCCCACTTCCCCTCGCGAGTGACAGGGCAGCTCGA
    GGTTTGACATGAATGGCAGAAGAGTTCACTTTCCGAACGCCCACTCAAGATTACGCCATCCATCTGCCAC
    AAAATATTACGCGCCTCCGCTTCCACTGAAGTGCTGCTTGCGGTGTCGCCTAAGCCCTGAAGTTTGCGTT
    TTCTGGTGCTTCTTCTGTCACGAGGGTGATGATGTAAGACTGGTCCTTTGCCTTTGAGTCCTCTATCGCC
    TCGCATCTGAACGAGAATGTTTTTGAAACAGGGACATTTTCCGGCACGTTGAGTACAGCAGGTTCTTTTA
    TGCCGTCACCCCCCGTCCACCATTGGTGCGAGTCCGGCACACCAGGAAAGAGGGCTTGGAGAGTCTTCTT
    ACTCGTGCAACTACTGCCTTTTCCCTCACAAACCAGCTTTTTGAAGTCTTCAGGGACCCAGTTCTTGGCA
    TTGTTGCATTCGATCGCCACCTTCCATCCAGGATACACATAAGCGATGTTCTCAGTGCCCGTCCCGCGGA
    TGGAACTGAACCACACGATGTTGTACTGTCCAAACGGAAACTGCTGCTCCTTTCGTGCATTCTTGAAAAA
    CTTTGGGGCGGTAGCTTGGTCTGCGTCCATTGCAGAGGCATAGGCAAATATGGAAAGAAGCATTGCTACC
    GAAGCGAGCTTCATTTTGTCAAGCAGAGCCAGGAAGCCTGGAACAATGAGGTTGGCCAGAGAAGACAACC
    TACTGACGTTGGTAGAGAGAGCTCAACAACACTACTGCATGCAAGAAAAAGATGCACGTTCCTCCCACTC
    AAACATCACTGGAACTTTTGTAATGCCAGGCAGCAGACTCACAAGAATCGATGTTGGTGCCCCGTGCAGG
    GAAAGATCAGTCACCCGCGTATGCGGGTAGGTTGGCTACTAAGACAGCGAAGACGAATGCCCATGAGAGT
    GGATTTTCCCGGACTCAAGGACAGCGGCTTGTCTGAAACGAGGCACATGAAAGAACAAAATTCGACGGCA
    ACTGTGCGTGTCCGGAGAGTCAGCAAAAAGTTCGACTCTCCAGCGTATACCTGCATTACCCTGCGCTCTT
    GGTCACCTCGGCTAGCCGCCTTTCTGCTTCGTGTGTGGCTCTCCCCGTTCTTGTGCCGCTGCAGTCGTTG
    ATCCAGAGAGGTGTCACCTGTGCTTCAGCGCGGGGGTGTACACTTGGTGTGAGTTTGGAAGCCTTACGTA
    AAAGTGGGGTCCAAAGTGCATTGATCCATGGATTACCAAACGAGTCGTAAAACCCCCGTCTTTGCCGTGC
    GTTCCCCGTCCGCGCGTTTTAGATTCGATGCTTCTCCGCGGAGTACGGAAACAAAACGAGCTTTACAAAA
    CTTTGGGCACTACTCCGGCATCCTTTGCGGCGATTCGTCTCCTTCCACTGAACGCTGCTGTAACTAATGG
    CGCGGTTACTGCCTGCAGAGAAATACCAGCGACACACTCCCGTTCGCTTTTGAGGCGCCCTGCGAGTTCA
    TTACCTCTACCGGGCGGGCACTATGGTATCGTCACGCGTCACGGTGCAGTCGGCGTGATGTCACCACACG
    CTGCCAGTGGATATATCTACGGAAGCTGTAAGTAGGGCAAGCGTTGTCACCTCTGTGCACTTCATCAAGC
    ACTCCGAATGAGAGCTTGCACCTAATTCCTCAATACGTAGGCAGAGGCGATGGAACCGTGTGTGTGCATA
    TACTGCCCCAGAGGGCGTGCGTGGAAGTCGGCGGTCCACCACGGGGCGGTCAGTCGTGTGCGTCGTGGAT
    TCACTTTTGCACAGTTCCGCGAGACTCCTGCAGAAGCAAAAAGTTAATTAAACATTGTTGTTCGTTAATA
    ATCTCCGCGCCTGAACAAGGGCATTTAGCGGGCACGACCAGACAAACTGCTCTTTAGCTGCAGTTCTTAG
    TGCCACCTTCACATTGCAGGATGGAAAAGAAACTAGACTCCGGGTAAAAGTAGAAGGATACAGGGATTCC
    CACAAAAAATTGAACTGTGCAAGGAATTGGTGTTTTATAGATGAAGCACGGACCAGAAAAACAGTTCGCC
    TCTCGTTACTCTAAACCGTAGCGTTGCATCGCCAAGCGGGGCGGCACGGTCTGAACTTGTCTACTCCAGT
    CGACCTTAGTGCTGCAGAGACATGTTATCTGCGGCTCCGCTGGCGTGCGTTGACATTTGCCAGAAAGTCT
    AAAGAGTTACTACACCATACAGCTTCGCAGAAATCCGCAACAGACGGGTGTCTTCGCTCTGGGAATGCAG
    GACAGTTGTCC
    
    
  • Protein Sequence : Show Sequence
    >XP_002368877.1 SAG-related sequence SRS42 [Toxoplasma gondii ME49]
    MKLASVAMLLSIFAYASAMDADQATAPKFFKNARKEQQFPFGQYNIVWFSSIRGTGTENIAYVYPGWKVA
    IECNNAKNWVPEDFKKLVCEGKGSSCTSKKTLQALFPGVPDSHQWWTGGDGIKEPAVLNVPENVPVSKTF
    SFRCEAIEDSKAKDQSYIITLVTEEAPENANFRA
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Oral immunization of mice with ZJ111/pcDNA3-SAG1 elicited specific humoral responses and stimulated proliferation of splenocytes (P<0.05). Mice immunized with ZJ111/pcDNA3-SAG1 displayed significant protection against an intraperitoneally challenge with 500 tachyzoite forms of T. gondii RH strain. Vaccination at 10(7) and 10(8)CFU per mice provided a 20% and 10% survival rate comparing 100% mortality of the non-immunized mice, exhibiting longer living time and better survival rate (Qu et al., 2008).
22. SAG2
  • Gene Name : SAG2
  • Sequence Strain (Species/Organism) : Toxoplasma gondii
  • NCBI Nucleotide GI : 8777958
  • NCBI Protein GI : 8777959
  • Protein Accession : AAF79156.1
  • Other Database IDs : CDD:309286
  • Taxonomy ID : 5811
  • Gene Strand (Orientation) : ?
  • Protein Name : surface antigen P22
  • Protein pI : 8.1
  • Protein Weight : 17781.73
  • Protein Length : 242
  • Protein Note : SRS domain; pfam04092
  • DNA Sequence : Show Sequence
    >gi|8777958|gb|AF249698.1| Toxoplasma gondii isolate C56 surface antigen P22 (SAG2) gene, complete cds
    AGTGACCCATCTGCGAAGAAAACGAGCGCTGCTTGCGATCCTGTGTGTTGTTTCACGGGTTGCAGTTCTA
    GGAACTGAGTTGTGATTGTGCACAATTGCGGTGTGACACCTTCTGTCTCGTTCCAATCTTTGTCTTGTCG
    GAACTATGAGTTTCTCAAAGACCACGAGCCTAGCGTCGCTAGCGCTCACGGGCTTGTTTGTTGTGTTCAA
    GTTCGCTCTTGCGTCCACCACCGAGACGCCAGCGCCCATTGAGTGCACTGCCGGCGCAACGAAGACTGTT
    GATGCACCCTCCAGTGGTTCCGTTGTCTTCCAATGTGGGGATAAACTAACCATCAGTCCCAGTGGCGAAG
    GTGATGTCTTTTATGGCAAGGAATGCACAGACTCGAGGAAGTTGACGACTGTCCTTCCAGGTGCGGTCTT
    GACAGCTAAGGTCCAGCAGCCCGCGAAAGGTCCTGCTACCTACACACTGTCTTACGACGGTACTCCCGAG
    AAACCTCAGGTTCTCTGTTACAAGTGCGTTGCCGAAGCAGGTGCTCCCGCTGGTCGAAATAATGATGGTT
    CTAGCGCTCCGACGCCTAAAGACTGCAAACTCATTGTTCGCGTTCCGGGTGCCGATGGCCGTGTCACATC
    TGGGTTTGACCCTGTGTCTCTCACGGGCAAGGTTCTTGCTCCCGGTCTCGCAGGTTTGTTGATCACGTTT
    GTGTAAAAGAAAGGGCTGATGATTAAGTAGTCAAAAGGTCACACCAAGATATGCACCCCTGAAGAGACGT
    CTTTGGGTCAATGGCCTTCTTTCGGAGAAGAGTTGCGAAAGTCGGTGAACGGTGGTCGCCCTCAGGGGTG
    CACTTTGTTGTTTTGCTTGAAGATGCTCTTCCTATAAATCTTCCGAAAATGTTTCTCCGCATGACGGGGG
    TTGAGCGAAGTTGGTGGTAACGGGAATAATTAAACGGGGATATCATTCCCCGTAGCGGGGTGGATGGGTG
    CCTCCGGGGCTGCCGCAGTTCTGTTCTCCGAAGTGACTCCAGGTGGGCCGTGCGGCTTTTGTCTTTTTCG
    TGAGAGCGGTCCGACCCTGCAATTCTCATGCCTCCGCTTCGAAATTGTCGTGCAAAGAAAACGTTGTTGA
    GGGGTGGGATTGCGATTGTTCTTGGCGTAGATCCTGGGAGGAACGCGAGAAAATGTATTTCCGGATCTGC
    GATTATGTGACAGAGGAACTTGTTTGCCCACACACTGCTGCAGTACTGTGCTTTTCCGCCAGACACAACC
    AGTGTTCCGGCGGGTGTGCCTTCGTGAAACGTTCTACACATAGAGAC
  • Protein Sequence : Show Sequence
    >AAF79156.1 surface antigen P22 [Toxoplasma gondii]
    MSFSKTTSLASLALTGLFVVFKFALASTTETPAPIECTAGATKTVDAPSSGSVVFQCGDKLTISPSGEGD
    VFYGKECTDSRKLTTVLPGAVLTAKVQQPAKGPATYTLSYDGTPEKPQVLCYKCVAEAGAPAGRNNDGSS
    APTPKDCKLIVRVPGADGRVTSGFDPVSLTGKVLAPGLAGLLITFV
    
    
  • Molecule Role : Protective antigen
  • Related Vaccine(s): T. gondii DNA vaccine pSAG1-ROP2-SAG2
23. sdfa
  • Gene Name : sdfa
  • Protein Name : SRS domain-containing protein; SAG
24. SOD
  • Gene Name : SOD
  • Sequence Strain (Species/Organism) : Toxoplasma gondii
  • NCBI Protein GI : AAC63943
  • Other Database IDs : CDD:223678
  • Taxonomy ID : 5811
  • Protein Name : superoxide dismutase
  • Protein pI : 7.07
  • Protein Weight : 21705.88
  • Protein Length : 258
  • Protein Note : Superoxide dismutase [Inorganic ion transport and metabolism]; COG0605
  • Protein Sequence : Show Sequence
    >AAC63943.1 superoxide dismutase [Toxoplasma gondii]
    MVFTLPPLPYAHDALAPHISSETLQFHHGKHHAGYVAKLNGFIEGTAFAGKTLEEVIRTSTGAIFNNAAQ
    VWNHTFYFSSMKPPMSGGGGEPTGRLLDEIKKEFTSVENFKDEFSKVAAGHFGSGWAWLVWDKQGKKVGI
    EQTHDAGTPITEPMKVPLLCCDVWEHAYYLDRKNDRPAYIKAWWNVVNWDFASKNLENALK
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Liu et al., 2017)
25. UP
  • Gene Name : UP
  • Sequence Strain (Species/Organism) : Toxoplasma gondii ME49
  • NCBI Gene ID : 7900273
  • NCBI Protein GI : 237830025
  • Locus Tag : TGME49_110640
  • Genbank Accession : DS984727
  • Protein Accession : XP_002364310
  • Taxonomy ID : 508771
  • Gene Starting Position : 1252500
  • Gene Ending Position : 1256433
  • Gene Strand (Orientation) : -
  • Protein pI : 7.12
  • Protein Weight : 31157.07
  • Protein Length : 303
  • DNA Sequence : Show Sequence
    >gi|212659389:1252500-1256433 Toxoplasma gondii ME49 scf_1112359874864, whole genome shotgun sequence
    GCGTCCCCCGCATTTCGGCAATCCGCGTTTGAACGAAACAATTGCTTGAGCAGCACGGAACTGCGTTGGC
    TCTAAAGGCCGCCTGGTTGTACATATACTTTGTGAAAGCCTTCCTTTCCGCCGACCGTCCACTCGAGGCA
    AAAACATGGGGGCGGAGAAAACCTGGAGAGGCACGGAAACGTGTGTACGTCGCCCCACTCATCTCCAGAA
    TCTCTATCCCACATCTGAAACCCGCTGACGGTGCCTTTGTTGCGTTTTTGTAGGCCGGCCGCAGACGTCA
    GTTTCCAGTGCGGCACGTGGACTGCGAAGCGCTCGGCGGTCCAGTGAACATTTCCAAGCAGACAGCCTCT
    GTGTGGACTGCTCTGGACCGGGAGGAGAAATTTACGCTCTCTGTGCACGTAACTAATGACGAAGAACCCC
    TAAAACGCTCACACGCAGTCTCGCGGTGCTTTTTTCGGGAGCAGCTCTCCTGCGTGGGAATGAAACCCAT
    CATTTGAATTCATGACACGAGGGCCCAAGGCAAATATGGAACCCCAGAACCACCGTTCCGTTCTCTCTGG
    TAACGTGGTACTGTGTGCATCATCTGGTTACAGTCCTAGAGTGCGCAGCGAGGACGACGTCTGCGCGAGG
    GCGCTGGATTTGCTCCGTTCGTCTTTGTGCGCCGCGTATCCACAGTACTTTCATCACCGGCCTTGATGCC
    TCCGAAGTTGCTGCTCATGACCTGCTCAGTTGCTTTACGCCGCAGGCTTGATGATGCCTTCGGAAACAAG
    TGTCACAAGGGCATCAAAGGCAGCTTCGCCCGCTGCCAAAGTAGCCTTATCTTGGTCTTCCGCCTAAACG
    CGACACACACACACAAAAAAAAAACAAGAAAAGTACAGAGTGACACAGCGTGGAAAGTCACTCCATCACC
    TAGTTTGCGCTTCGTTTTCGTTGACACAGGCGTCGGCGCCTGTTTGCGGCGGAGTCTGTTTTGTCGTATC
    TCCATGACACAACACTGTCTTTTCTTCTCTACCGAGGCAACCCAGAAGGAGAGTGTGACCTCACTCCTTG
    ACATTTCTCCCCTCCTCCTGTTTCCGCTCACCGTCACGTGAGCTGTCAAGTTCGGATTGCTCCGGTTCAC
    AAGGCCGATCATGAGAGAGGCGGCGTACGACTTGGGTCCGTTTGGGATGCTGTGCTGTCTCTGGTGCATG
    AGATGGAAGATTTGGTGCGTCTCCATCTCCAGACTCACGACCCCCAGGTCTGTCATGTCCTTCAGGAGGT
    TCGCGTTATTGTCGTTCCACAGCGGCAACTCTCTGCCCTGAATCGCATGCACACAAGAAACACGGCATGA
    CCACACATTCACCTTCTCATCCTCTTACCGGCAATCTTTCGAGGTTCCCTTTACCTACGCGTCAAGCGCA
    CTGGTGCGATCACGCGTGCGATAGCGCGAAGGAAAACACGAATCCAGCAAGCCTCGACAAGTGGTGAGGT
    ATGTGTACCTGCGCCAGGCATCGCCGATGCAAAAGGGATGAGGAGGACCCTCGGGTCCCTCCTCAATCGG
    TGTATGTGAAAAGACACTGTCCCTCCCTTTCGGCTAAGGTTCCACGCCTTCGGTGACTAGACTATATGCG
    CTATACACACAAGGTCAACAAGTGTCAAAGTTTGGCCATCAACAGCCAGTGCCAAAAGCACATACCCGCG
    TACCGCAGGCGGGGCAGCCGGTAGGCGCTGGTCGTTACCGCCCCCCCCTTGTGTGCCACCTTGGTCCCCC
    CTTTCGGAGAGCGCACACGAGTCAGTACAGTCTTCGAAATCACCTTATTTGATTAACGTAACAGCGCAGA
    CGCCTACGGTGCACCGCATGTAAACTGTACATTGGATACGTTTCAGCACTTCAGGGTTGCGAGAACCTTA
    TAAATGAGAGAAGAGATGTTCGGTCTAGTGTTCCGTTATCCTACATAGATGACGTGGCTTGTAGCACGTT
    TTTGTATCGTGTTTGTAGAAACGGATTACTGCTCACCTGACAAGCGAAGAAAGTCTCCGCCGAGCAGTTG
    AGGCCTCGTTTGACTGCTGCACCCTGCGCATTCAAGTTGGCTTCGATAAGATCGGTCAAACGCTTGTCTG
    CCTTGACCGGCGCTGTCAGCAAGTAGGGTCGCGGGTCGCGCCCGCACTCCCGTTTGTTTCCCAAAGCTGC
    TTCGCCGTCAAAGTGACAGTAGTTCCTAGAAACACAAGCCGAAGCAAACCACCCCCTAGGTTTGTAGATC
    CGCGAGAGAGTGCGTGGATTCGACAGCGCACGGACACTTCCGGAGATGCGTGTGCACAACCCGCCCCGCA
    GCAGAGCACATGAACGACCAAGCCGAACCCGCTGCGGGTGCACAACACAGCAACTACACAATCAGTCAAG
    AGTTCCCGGCCAAAATGCCCGATCAACAATCTACGGCCACACATCAGCTGGGGGTACTTCGTCGTCGTAC
    GAGCAGTACCGTGGACACAGCCCATGTAGGGCTTTCTGCCTCTTTACAACCGTGAAATGCCCTTGTCAGT
    GAACCGGGTCGCAACTACGAGGTGACAAAAAAAACAGAACTGCACGCCTTTACCTCTGCCCTCACCTGTA
    GCAGTACATGCTCTCGTCGCTGAGCATGAGAGTACCCGGCCGGGTCGCGTAGTCGACGATTCCACAACTG
    CCAATGCGAACGACGGCCAAGGGTTCTCCTTCCAAAACAAAAGAGGCTTCACGAATGAAAAAATCTGCCA
    TCGGAGCACCCATGCCAATGCAGACGATCGACATGCGCTGTCCGCCTTTGATGTATTTGCCTGAAAACAA
    GCAGAGAAAAACGAATACTAGAGAGTTGTGAACGCCAAAAAACAATCCGAAGAAAGCCACCGCCGACCAG
    CAGGAGTTGCCACGAGCTGGAGGTCCTCGGGTTTGTCGAAGCACTATCATGCTATGACACCCCGAAGCAC
    ACGACTTAAAAGATCGTTCAAAGCTCCCTCACCCTGTTCGGGTGCTCCCCATACACAAGATCTACGTGGG
    GGTGCTCACGCGTCCAGTTCGGTCTATCATCAGTCTGTGGGTTTTCTAAACTCCCAAAAACGGTAAAGGC
    AACCGATACGCGTTTTTTCAGTCGATTCACTGCCTCTGTGCAGGTGTGGAAAGGGTCTTCCAACTGACGT
    CGCCAGGTCATCTGCCACCGTTCGCTCTCCCATGTTGTAGAATGCAAATCGCTACTTACCCGTGAAGGTG
    TAGAATTGTCGCGAAGAGGAAACCTCCGTGACATCCGTAAGGAATTTCGCTGCGAGAACCCGCGCGCGCT
    GTTCGCAGCCGACCGTCACGATCAGTGATGCCAAGTCTCCCTTCTTGACGCCCAGATGGTACGTGCGACC
    ATCCGGGGTAAGGAAGATATTCTTTCCTTTGAGTTCCGACTGTCAGCGACAAACACACAACCACAGGACA
    CGTCGTCATGCCCCCGTGCATAAGCTAGAAGTTTAGATAGCTAAATACCAGGTAAGCTCATGTTGAAAAC
    AGGCTAGAGGCAGAAAAAAAAGCCGGAAAAAGATGTGATCTGCAACTGGTAAAGATGCCACTCTTGAACT
    GGCAGGCACTACGCTCGTGTCCTGCACCACTGTATACGTCGTCATTGCTGCAAGAGAATCAACTACATAC
    TAGGCAAATCTACGGCAAAACCCCTCGTCAAGAACTGTGAGGGAATGGGGCGGAAGCGCCCGCCCTTTTA
    AATGCGACAAAGCCAGAAAGAGAAGCCCCGCTGCGTCAGACAAGAGGGGGAACGCAGGCGGACGCAGCCC
    CCCGTGAATCAACGCAGAGCGTAGATGCGTGCCCTATCTTGCTGAAACTCACCATTGTACGCAAAAACAG
    AGGCACTATATACACGACCTAAAGGATGGCGAAACGTTTCTTCACTTTTGTTTGGAAGACAAAAGGGAAT
    GTTCAGGCAGCAGC
  • Protein Sequence : Show Sequence
    >gi|237830025|ref|XP_002364310.1| uridine phosphorylase, putative [Toxoplasma gondii ME49]
    MSELKGKNIFLTPDGRTYHLGVKKGDLASLIVTVGCEQRARVLAAKFLTDVTEVSSSRQFYTFTGKYIKG
    GQRMSIVCIGMGAPMADFFIREASFVLEGEPLAVVRIGSCGIVDYATRPGTLMLSDESMYCYRNYCHFDG
    EAALGNKRECGRDPRPYLLTAPVKADKRLTDLIEANLNAQGAAVKRGLNCSAETFFACQGRELPLWNDNN
    ANLLKDMTDLGVVSLEMETHQIFHLMHQRQHSIPNGPKSYAASLMIGLVNRSNPNLTAHVTAEDQDKATL
    AAGEAAFDALVTLVSEGIIKPAA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A UP mutant, in combination with an OMPDC mutation, is attenuated in mice and induces significant protection from wild type T. gondii (Fox and Bzik, 2010).
  • Related Vaccine(s): Toxoplasma gondii OMPDC/UP mutant vaccine
1. Ccl2
  • Gene Name : Ccl2
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 20296
  • NCBI Protein GI : 6755430
  • Locus Tag : RP23-350G1.3
  • Genbank Accession : AJ238892
  • Protein Accession : NP_035463
  • Taxonomy ID : 10090
  • Chromosome No : 11
  • Gene Starting Position : 82035576
  • Gene Ending Position : 82037451
  • Gene Strand (Orientation) : +
  • Protein Name : chemokine (C-C motif) ligand 2
  • Protein pI : 10.38
  • Protein Weight : 14837.89
  • Protein Length : 148
  • Protein Note : Also known as JE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594
  • DNA Sequence : Show Sequence
    >gi|372099099:82035576-82037451 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
    TGCAGAGAGCCAGACGGGAGGAAGGCCAGCCCAGCACCAGCACCAGCCAACTCTCACTGAAGCCAGCTCT
    CTCTTCCTCCACCACCATGCAGGTCCCTGTCATGCTTCTGGGCCTGCTGTTCACAGTTGCCGGCTGGAGC
    ATCCACGTGTTGGCTCAGCCAGGTGAGACCCCCCCAGCTCCCTTCCCAGCATATCACCCCATTTTTGAAT
    TGTCGTGGATTGTGATAGCATAGTCTCACATGGTCAGGTACTTTTTTTTTTTTCTTTAACCAAGATAAGG
    AGCATAAAGAAGGAAGGACAAAGAGCCAACCCAATTACAAGATTGCTTCTGGAAAGCAACTAGAATTTTA
    ATTGTTAGATCTAAATTTGGAATCACACCTTCATATAGTTCCTGTTCCAGTTACTTCCCTCAGTATTTGG
    GAACCTGGGTGATCAAACAGAGGCTTGGGTTGGTGCCTTTTTCCAGATAGAGGAGAAAGGGGAAGAGATC
    CAAGATCCGAGCTGTGTTTCACCCAGCCCTGCTTCCAGAGATAGCAGCTTAGCGGAGGTGGTTGGGATCA
    GAGATACTCATGATTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAGCCTACAGTAATGTA
    CTCAGGTAATCTTCTCAGGTCATAGTAATTTGACTTCTAACTCCCCCAAATGACAGTCCCCAGAGTCACA
    TAGTTTTAATGGCATCCCTCTACCCAAGACTGTGAGCCTACTTTAAGCTGCAAATAACTGAGTCTGTTGT
    CAAAGATCACATCCCAGATCTGATGTATTGGCATTTATATCCCATCCTGCTGAAACTGCCTTCTCCCCGT
    GGTCCTTCTCTTCTCTAAGGTCAGAAGCATCTTTCCTGTCCTAATGTGCTTCTCTTTTACTCTCCAGATG
    CAGTTAACGCCCCACTCACCTGCTGCTACTCATTCACCAGCAAGATGATCCCAATGAGTAGGCTGGAGAG
    CTACAAGAGGATCACCAGCAGCAGGTGTCCCAAAGAAGCTGTAGTGTGAGTTACATACCCCGGCCCTCCC
    TGGTCCAAAGGTTTTTCCTTAAGAACAAGGGATGGTCCTCATATACTTATAGTCAGTCACACACTCAGAT
    CCAATGGGGAAACCAAGGCCAAGAAGGCAAAGGCAGTTCTCAACAGCATTGTCTCTATGGCTGCTGTTCA
    GGCCCTTTCTACTCCACAAGCTTATCTTAGAAAACCTGCAGGAGAAGCAGGTCACTTTGAGTCCCCTTTT
    TCTACCTGCCCTCCCCCGCTGAGCTCTACACAGCCCCTCCATGTATACCAGACTGAACTTCATCTAACAG
    TGTCTTTTCTCTTCCCACAGTTTTGTCACCAAGCTCAAGAGAGAGGTCTGTGCTGACCCCAAGAAGGAAT
    GGGTCCAGACATACATTAAAAACCTGGATCGGAACCAAATGAGATCAGAACCTACAACTTTATTTAAAAC
    TGCATCTGCCCTAAGGTCTTCAGCACCTTTGAATGTGAAGTTGACCCGTAAATCTGAAGCTAATGCATCC
    ACTACCTTTTCCACAACCACCTCAAGCACTTCTGTAGGAGTGACCAGTGTGACAGTGAACTAGTGTGACT
    CGGACTGTGATGCCTTAATTAATATTAAACTTATTTAACTTATTGATGTTATGGTATTCCCTTTCATGAA
    TACTAAAATTTCTTAAATGCAAGGTGTGGATCCATTTTTCCCTCTCTGTGAATCCAGATTCAACACTTTC
    AATGTATGAGAGATGAATTTTGTAAAGATGAATGGGTAAACTTTGTGTTTGAGATTCCAAGGTATTGTTT
    AAAATATTATTATGGATATTCCTTATTATTAAAAGAAATATATTATTTTTGTACAC
  • Protein Sequence : Show Sequence
    >gi|6755430|ref|NP_035463.1| C-C motif chemokine 2 precursor [Mus musculus]
    MQVPVMLLGLLFTVAGWSIHVLAQPDAVNAPLTCCYSFTSKMIPMSRLESYKRITSSRCPKEAVVFVTKL
    KREVCADPKKEWVQTYIKNLDRNQMRSEPTTLFKTASALRSSAPLNVKLTRKSEANASTTFSTTTSSTSV
    GVTSVTVN
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Toxoplasma gondii mic2 mutant vaccine
2. Ifng (Interferon gamma)
  • Gene Name : Ifng (Interferon gamma)
  • Sequence Strain (Species/Organism) : Mouse
  • NCBI Gene ID : 15978
  • NCBI Protein GI : 33468859
  • Genbank Accession : NM_008337
  • Protein Accession : NP_032363.1
  • Other Database IDs : MGI:107656; UniProt: P01580
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • DNA Sequence : Show Sequence
    >gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
    ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
    GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
    GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
    AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
    AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
    GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
    AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
    GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
    AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
    AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
    AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
    TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
    TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
    ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
    GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
    AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
    TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
    AGTATACCTTTGCAACC
  • Protein Sequence : Show Sequence
    >gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
    MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
    ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
    LLPESSLRKRKRSRC
  • Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
  • Additional Molecule Role : Vaximmutor
  • Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
  • Related Vaccine(s): Toxoplasma gondii mic2 mutant vaccine
3. IL-6
  • Gene Name : IL-6
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16193
  • NCBI Protein GI : 13624311
  • Genbank Accession : AC112933
  • Protein Accession : NP_112445
  • Taxonomy ID : 10090
  • Chromosome No : 5
  • Gene Starting Position : 30013160
  • Gene Ending Position : 30019967
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 6
  • Protein pI : 7.5
  • Protein Weight : 23291.86
  • Protein Length : 211
  • Protein Note : Also known as Il-6
  • DNA Sequence : Show Sequence
    >gi|372099105:30013160-30019967 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
    ACCAAGAACGATAGTCAATTCCAGAAACCGCTATGAAGTTCCTCTCTGCAAGTAAGTGAAGGCAGTTCCT
    TGCCCTCTGGCGGAGCTATTGAGACTGTGAGAGAGGAGTGTGAGGCAGAGAGCCAGCATTGTGGGTTGGC
    CAGCAGCCATCAGCTAGCAGCAGGCGCCCAACTGTGCTATCTGCTCACTTGCCGGTTTTCCCTTTTCTCC
    ACGCAGGAGACTTCCATCCAGTTGCCTTCTTGGGACTGATGCTGGTGACAACCACGGCCTTCCCTACTTC
    ACAAGTCCGGAGAGGAGACTTCACAGAGGATACCACTCCCAACAGACCTGTCTATACCACTTCACAAGTC
    GGAGGCTTAATTACACATGTTCTCTGGGAAATCGTGGAAATGAGAAAAGAGGTGGGTAGGCTGTGAAACT
    GATGAAGACCCAGTGTGGGCGTCCATTCATTCTCTTTGCTCTTGAATTAGAAATTCTCTGCTGGGATCTA
    GGGCCCTTAGGATTTGAAGCTAAAGGTCAGACTAGACTGTGTTCTCTCTTTCTCACCTCTTTGCTGGTTT
    TGAGTGGAGGTTGGGAAGGGGTTCCTTTCCTGTCTGGAAGATACAGAATGTGACTGCATTTCTAGAAAAT
    TCACAGTGGGCCATTCTCTGTTCCAATTTAGCTATTGCTTCTTAGGTGGGGATTCCTTTTCCCTCATTAT
    TCCTTTTTCCTTATCTCTTTGCCTTCATTTATCCCTTGAAGTCTACAAGGACCAGAAAGTGCTTTTTGGC
    TGAATGTAGTAAGTTCTGTGTGTGTGAAATCAGGATGCTCTAGGGTCAGCCCAGATAAGATGCATATTGT
    ATAGAGCCCAATAAAGTGCTAACACACACACACACACACACACACACACACAACCTAGATTAGGCTGGGC
    ATGGTGGTGGATGCCTCCCAGCACTTGGGAGGCACACGCAGGAAGATCTCTGTGAGTTAGAGGCCAGCTT
    GGTCTACATATTGAGTTCCAGGATATCCAAGACTACATTGAGAGATCCTTTCTCAAAAAAAAAAAAAAAA
    AAAAAAAAACAACAACAACAAACAAACAAACAAACAAGAAAAAAAAAACCCAACCAACCCTACCTAGATT
    AAAATATCTCCCATCTTTAAATGAAAAGTATTTTGACTCTGCTTGACAGAAAGGAAAAAAAAAATCACAA
    ACAAGATTAATAGCCTAGGTCTGGGTGTGTAGAACCAACTTTGTAATCTTGGGAAAATCCTCAACTATTT
    AGTACTCTTTATCTGCATAATTATGGAGTTACAGATAATGGCACAGACCCTTCCAGATGGAATATTCTGT
    GACTTAAATTTATTTGCCCCAAGATATCATCAAAATTTCTGTCCTTTACCAAGTTCCTACTCTATGCTGG
    GCAGTTTTTATCGTTGAATCCTTGAGACAAATGTATAAGAGGGACATCAATAATTCTTTATTGAAACATA
    GGAAATACTTAGGAGAGTAACAAGCTTAAAGCCAAGGGACTGAGCCTAAGGGTGCATTTTTATTTATTGG
    GCTGCTTAGTAGGGTTCTAAGAAGGTATAGCTTCCCTTTTCTGTGTCCTGGCTATGAAAGAGCATGACTT
    GGCCCTCTAGTGGTGCTTGTTGTAAGAGGTGTTTAAGTGATTGCTCTGGTAATCTTTTGCAGTTGTGCAA
    TGGCAATTCTGATTGTATGAACAACGATGATGCACTTGCAGAAAACAATCTGAAACTTCCAGAGATACAA
    AGAAATGATGGATGCTACCAAACTGGATATAATCAGGTAGAAACTTGTCACGTTTACTTTCAACAATTCT
    TCAAGGTTCTCTCTTGCACGTGATACCTGCATTTGTACAGTCCCAGTCAGGCAACAAAGATGGGGGGATA
    CATCCAATACCACTTGGATGTAACTGGCCTGCAGTAGCTTGAATACAAAAGGAGATTGAGAGGTACCTCA
    GCAGCTGCTAGGGTGAGGGAAAGTTTACCAACCAGTGCTATAAATGTTCACTCAATCTATGTCCATCAGG
    AGCTAAATGTTTAAAGTCTCTATTCTCATCTTGGCTTTGAGGTGTTAGATGATTAAAAAAAAAACATTTT
    TTTTGTCTAAAAAAGTGGAAGAGTAGATATAAAACATGTTATGTAAACGTCATGAGGAAAGTAACTTACA
    GATTTAATTAAAAAGCAGTTTATTTAAACAGGTAAAGGCCCACTATGCTGGGATTCTTCACCACTGTTAA
    ATGTTAGAAGTCATAGCAAGTTTAGCTAGTTCATGAGCCATCTCTTCTCTGGCTGCCCCTGGAAGGATCC
    AGGCCTACACTCCCTACTGAGAACACTCTTCACACCCCTCTCCTTCCAGCCGGTGAGATAGTCTACATCA
    GGTCTCAGGACAAGTCAGACAAGAACAAGAGTACAGGAAGGGCACTGCAGGATAGAAGGGAAGGAGTGAG
    GGTCAGAAGTGGATTCCCACTGCCACACTCTGGGGTAGGGGGTGGGGGGATATTAAGTGGTGCCTCTCTC
    TACTTGTCCAGATAATTCAGGAGGTCAGCTGATGCTGCCTGTTGTCTAATTCTGTAGAAGATACTGAACA
    GCAGGAATCTTTTTTTCAATTTTTTATTAGATATTTTCTTCATTTACATTTCAAATGCTATCCCCACAGT
    CCCCTATACCCTCCCCCCCCTCCCCGCTCCCTTACCCACCAACTCCCACTTCTTGGCCCTGGCATTCCCC
    TGTACTGGGGCATATAAAGTTTGCAAGACCAAGGGGCCTCTATTCCCAATGATGGCTGACTAGGCCATCT
    TCTGCTACATATGCAGCTAGAGATATGAGCTCTGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTAT
    AGGGTTGCAGACCCCTTCAGCTCCTTGGGTACTTTCTCTAGCTCCTCCATTGGGGGCCCTGTGTTCCATC
    CAATAGAAGACTGTGAGCATCCACTTTTTGCCAGGCATTGGCCTAGCCTCACACGAGACAGCTATATCAG
    GGTCCTTTCAGCAAAATCTTGCTGGTATATGCAATACTGTCTGGGTTTGGTGGCTGATTATGGGATGGAT
    CCCTAGGTGGGGTAGTCTCTGGATCTTCCATCCTTTTGTCTTAGCTCCAAACTTTGTCTCTGTAACTCCT
    TCCATGGGTATTTTGTTCCCCATTCTAAGGAGGAATGAAGTATCCACATGTTGGTCTTCCTTCTTCTTGA
    TTTTCTTGTGTTTTGGAAATTGTACCTTGGGTATTCTAAGTTTCTGGGCTAATATCCACTTATCAGTGAG
    TGCATATCAAGTGACTTCTTTTGTGATTGGGTTACCTCACTAAGGATCATACTCTCCAGATACATCCATT
    TGACCAAGAATTTCATAAATCCATTGTTTTTAATAGCTGAATAGTACTCCATTTGTAAATGTACCACATT
    TTCTGTATCCATTCCTCTGTTGAGGGACATCTGGGTTCTTTCCAGCTTCTGGCTATTATGAATAAGGCTG
    CTATGAACATAGTGGAGCATATGTCCTTATTACCAGTTGGAACATCTTCTGCGTATATGCCCAGAAGAGG
    TATTGCTGGATCTTCCGGTAGTACTATGTCTAATTTTTTGAGGAACAGCCAGACTGATTTCCAGAGTGGT
    TATACAAGCTTGCAATCCCAACAGCAATGGAGGAGTGTTCCTCTTTCTCCACATCCTTACCAGCATCTGC
    TGTCACCTGAATTTTTGATCTTAGCCATTCTGACTGGTGTGAGGTGGAATCTCAGGGTTGTTTTGATTTG
    CATTTCCCTGATGATTAAGGATGTTGAACATTTTTTCAAGTGCTTCTCAGCCATTCAGTATTCCTCAGTT
    GAGAATTCTTTGTTTAGCTTTGTACCCCATTTTTAATGGGGTTATTTGAATTTCTGGAGTTCAGCTTCTT
    GAGCTCTTTGTATATATTGGATATTAGTCCCCTATCAGATTTAGGATTGGTAAAAATCCTTTCCCAATCT
    GTTGGTGGCCTTTTTGTCTTATTGACAGTGTCTTTTCCCTTACAGAAGCTTTGCAATTTTATGAGGTCCC
    ATTTGTCGATTCTCGATTTTACTGTACAAGCCATTGCTGTTCTGTTCAGGAATTTTTCCCCTGTTCCCAT
    ATCTACGAGGGTTTTTTTCCCACTTTCTCCTCTATAAATTTCAGTGTCTCTGGTTTTATGTGGAGTTCTT
    TGATCCACTTAGACTTGAGCTTTGTACAAGGAGATAAGAATGGATCAATTCGGGCTGGAGAGATGGCTCA
    GTGGTTAAGAGCACTGACTGCTCTTTCAGAAGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGTTCAT
    AACCATCTATAATAAGATCTGATGTCCTTTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATGTA
    ATAAATAAATAAAACTAGATTATTTAAAAAAAAAAAAAAAGAATGGATCAATTCACACTCTTCTACATGA
    TAACCACCAGTTGAGCCCGCACCAATTGTTGAAAATGCTCTCTTTTTCCCACTGGATGGTTTTAGCTCCC
    TTGTCAAAGATCAAGTGACCATAAGTGTGTGGGTTCATTTCTGTGTCTTCAATTGTGTTCCATTGATCTA
    CCTGTCTGTTGCTGTATCAATACCATGCAGTTTTTTTTATCACAATTGCTCTGTAGTACAGCTTGAGGTC
    AGGCATGGTGAGTCCACCAGAGGTTCTTTTATTGTTGAGAATAGTTTTTGCTATCCTAGTTTTTTTTTTT
    TTTTTTTTTTGAACAGCAGGAATCTTATTCTCAAATTGAATCTATTCCTAGAAAGAACTGACTTCCTTTT
    CCATTTTACTTATAGGAAATTTGCCTATTGAAAATTTCCTCTGGTCTTCTGGAGTACCATAGCTACCTGG
    AGTACATGAAGAACAACTTAAAAGATAACAAGAAAGACAAAGCCAGAGTCCTTCAGAGAGATACAGAAAC
    TCTAATTCATATCTTCAACCAAGAGGTGAGTGCTTCCCCATCTCTCATGCAGTGTGGGAAAGAGGACACC
    CGGCACCTCAGGGTAGCGGCACTTTTTCCAGACAGCTGCTCAGAAGGGAGAGAGTCTGAACAACAGTTCT
    TGATTATAAGGCCTTGCTTTGTTTTACTTTGGGGTTTTGTTTGAGGTTCTCTTTTGCAAAGAACATCAAT
    ACCTGCTTTAAACTGTATTAATAGAATGTTACTAATTGCGTAAGAGGTATGAAAACTATGACAGCCATAC
    ATAGTCACCCATTATGAGAGCACAGAGACAAAAGTGACTTTAATATTTAATCCTTGGCAAGTGACATTTT
    TGTAACCAGAGTTCTAATGCAGAGAAGTAGCCAAAAGCTAAAATGTCAGGGAAGGAAGGGATCAAGGGCT
    TCTGGCTACCATTAGCCAGAAGAATGGTAGATATGGAGGATTAATGACTCAGAGTGTGGGCGAACAAAGC
    CAGATGCAATAAGAAGGGCCTGGAATGAAACCCTCTTGCTAAGGCTGCTTTTGCCACTTGTAGTTTCTTG
    CCTTAAACCAGAGAGTTTCCCAATTTAATGTGCACAGGAACCACTTAGGGTCTTGTTCCAGCAGGGTCTT
    ACTTAGGAGGTCTAGGAAGGGAACTAAGATTCATTTTTGTAAACAGCTGCTTGTTTATGACCCTGCTATA
    TAGTATTCAGGCTCCTAAGTAGTGGGGCAAGCCTTCCAGTTAGTCTTCCCCATGCCAGTGGGCCCATGGA
    AGGGTGTTTCCAGACTTCTTCATGCTACCACACTAAGAGACTCTCAACAGAGTCTGAATGGAAACCACGA
    AGGAACACATTTGTTTTAGATTCCTCTGTGCCACCTTTACTGATGGGAGCTTCTGTTTTCCAGTAGAATA
    CAGTATGTTAGCATGGATGCTTGGATAACAGACAAATAAGATGGTGGTGCTGACCTCTGGACGCTTACTC
    TCTAGTGGCAGACAGAACAGTAAGGTTAGAATTCTGTTGCTATTAAAAAACTAATAATAATCACCTTGAA
    AAAGAATGGAGTTGTTAGGCATGGGTCTCTCTCGAGTAAGCTTGGAACAAAAGCATCTCCCCTGGCTTGG
    GTGAGTCAAAGCAGATGGACTTAGCTCGTCTCATTCATTCTAAATTAGAACTTCTTCCCACAGCCCAGAA
    CACGCCACAAGAAAAAAAAAATGTGCAATATTTAACCAGTCTTTGTTTTTTCCTCCTTTAGGTAAAAGAT
    TTACATAAAATAGTCCTTCCTACCCCAATTTCCAATGCTCTCCTAACAGATAAGCTGGAGTCACAGAAGG
    AGTGGCTAAGGACCAAGACCATCCAATTCATCTTGAAATCACTTGAAGAATTTCTAAAAGTCACTTTGAG
    ATCTACTCGGCAAACCTAGTGCGTTATGCCTAAGCATATCAGTTTGTGGACATTCCTCACTGTGGTCAGA
    AAATATATCCTGTTGTCAGGTATCTGACTTATGTTGTTCTCTACGAAGAACTGACAATATGAATGTTGGG
    ACACTATTTTAATTATTTTTAATTTATTGATAATTTAAATAAGTAAACTTTAAGTTAATTTATGATTGAT
    ATTTATTATTTTTATGAAGTGTCACTTGAAATGTTATATGTTATAGTTTTGAAATGATAACCTAAAAATC
    TATTTGATATAAATATTCTGTTACCTAGCCAGATGGTTTCTTGGAATGTATAAGTTTACCTCAATGAATT
    GCTAATTTAAATATGTTTTTAAAGAAATCTTTGTGATGTATTTTTATAATGTTTAGACTGTCTTCAAACA
    AATAAATTATATTATATT
  • Protein Sequence : Show Sequence
    >gi|13624311|ref|NP_112445.1| interleukin-6 precursor [Mus musculus]
    MKFLSARDFHPVAFLGLMLVTTTAFPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELC
    NGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKAR
    VLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQ
    T
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Toxoplasma gondii mic2 mutant vaccine
4. Il12b
  • Gene Name : Il12b
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16160
  • NCBI Protein GI : 6680397
  • Locus Tag : RP23-388G23.1
  • Genbank Accession : AL607030
  • Protein Accession : NP_032378
  • Taxonomy ID : 10090
  • Chromosome No : 11
  • Gene Starting Position : 44400062
  • Gene Ending Position : 44414016
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 12b
  • Protein pI : 6.5
  • Protein Weight : 35952.67
  • Protein Length : 335
  • Protein Note : Also known as p40; Il-12b; Il12p40; Il-12p40
  • DNA Sequence : Show Sequence
    >gi|372099099:44400062-44414016 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
    CAGAAGGAACAGTGGGTGTCCAGGCACATCAGACCAGGCAGCTCGCAGCAAAGCAAGGTAAGTTCTCTCC
    TCTTCCCTGTCGCTAACTCCCTGCATCTAGAGGCTGTCCAGATTCAGACTCCAGGGGACAGGCTACCCCT
    GAACCAGGCAGCGTGGGAGTGGGGTAAGTGGATTCTGGGAGCATCTCGGATGGCTTTCCCCGCTGGTGGA
    AACCAGGGGCTTTGACGACAGGGGCTTCAAATAGTCATTTAAAAAGTCAGCTAATTACAGATGTGATGGC
    GCACACCTGTAATCTCCAAATCTGGGAGGCAGGAAACAGAATGCACAGGAGTTTAAGGCCAGATTCAGCT
    GTGTAATGAGTTTGAGAGAGTCTCAAAAAATTGAGCTACTAATAATATGTGCCAAGCATTGTGGACAGAT
    GTGTATTTGATAGAAAGGTTTGCTTTGTCTATATAGACACATATACTATGTGCATTTTCTTAAGTATTAA
    TGCATATGTGTTAAGGAGTGTTGTTAAAATTCTGTTCTTGCCTTAAGAGTGAAACTTAAAAAAAAAAAAA
    GCCTTTCATGTACCCCTTCAAGCCTAAGCTCATCACTGAATGTCCTACCTGTGATGGCCTTTAGAATCAG
    CTATGATAATCAAAGTGGAGAGCTGGGTTTGGGGGTTCCTTTGGATTTTAACTAACTTTGCCACCTCTTC
    AAAACTGGAAAGCCCAAGGCATGTTCCAAACTATTTCCTAAAGGGTTCTAAAATCTGTGAGAAAGGGAAG
    GCTAATCTTCATGGGTTGGCTTCCTCTGCTTGCCCAGGTGAAATCATGAGCACCAGATGGGGCAGGGTGA
    TGGGGCAGGGTGACATCTTGCTTTCACCCAGGGATCACTGAGCCTCAGCTGGGGGCTCCGTTTGTTCAAG
    CAAGGCATTTCAAGATGAAGTTAGGTCACCCCCAAGAATTCATTTTGCTGAGATCTGAAATACTGTGATT
    ATTGCACCAACAATCCATGGACTCTCTCTAGGCTAGATTCTGGATATAAGACTAGACAGATATGACCTCT
    GTCACACACCTCGGTATGGGAAAGGGGGGACAGACATTAAAGACGTAATCAAATGATTGTCATTTCAGCG
    ATGCTGAAGGGGAAGAGTAGCTATTTGGGGTCCTGTTTTCATTTGGGGCTCAAGCTGGCACTTTCTGTGG
    ATCATTTCATGAACTCCTAACCTGACAGAGTTATTTTTAATCTAGGAGTACAGGCTTGACAAAGTCAACA
    CAACTTGTCAAGGTCACATAACTAACAAGTGGCAGACTGAGGTCTGTTTGGCTGTAGAACCAAGGCCCTT
    TGCCCGTGCCCTCCTTGCCATCCCTGACAGCCACGCCATCTGTACTGTGCATTGTTGTTTCTGGGCATTT
    TTATTTTTACATATCATTTCTCTTTTAAATTAAAGAATAACAGAATGAAACTTGCAAAAGGGATGTCCAC
    TCAGGCACCCGCTGGCAGCAATGTTTGGAAGCATTTGCATGCTCTAGGGAGCTAGAGTATCCAGCCCAGG
    GTTCCTGTGCTCTTTGCACAAGACTTTCAGGACTTAAGAAAACTAATGAAAACAAACAAACAAACAAACA
    AACAAAAAAGAATTGTTAAAACATAGCATATGGAAGAATTGTGGAGTGAGAGGGAAACAAAGCCCAGCAT
    CTGCCAATCAGCCTGAAAGCCAAAGCCTGGCTACATACTGCACACTTTTTTGCTTGTGCAGATTTGATTA
    AGAGGCGACCTGCCATCATCTCCACCCTCTGCTGGTCTTCTATGATCTAAGATGTAGGTGATGCCGAAAT
    GAAGATTGTTCCTTGGACCATTGCCAGGGCTCCCTTCAGCCTGAAATATGGTTCACGTGTGATGCTCAAG
    TGCATCTGAGGCTGACAGAGGAAATGCTTGGCATGTAGCCAAAGGGGAAATCCCTGTGTTGAGAGCATAT
    GAAGAGATCACCAGGAGATGCCAGGAATCTCTGCCTTTTTTCCCTGCCCAGAGTCCTACCATCTGGGTTT
    CACAGCCGTGTATAATTGCATCACCTTCTCTGGGCATTTCTCTCTACAGCCTGGCAGATTTTTATTTTTC
    AACCCAGTGTGCACAAAACAGAAAGAATTTCAATTTTTTTCCTTAGCTTTTTACTAGTGCAAGGTATTAT
    ACAATGTCATCTCACTGCATGCCCAATAGCTCTTGAAGATCACATAAGCCTTATGTCTTTTTATATGAAG
    GCAGTCGGGCTCAGAGGAACTAAACCAACACCAAACAGCTACTGAGTAGTAGGGCCAGAATCCAAGTCCA
    TGTCTGCTTGACTCCAAGCCTATGAATGTAGCAACCAGCACCATTTCAGCTTGTACTTGTCTTGACTATA
    TGATAATTTGGACCTAGACATGTCCTGACTCCTAGTCCTGTCTCTGTCATGAAAAAGAAATCATAGCAGG
    GCCCTCAAGAGGTTAGCAGTACTTCTGTTCTCTCAAAGGTCGTGATTTCTGACCAAGGCCTGTTTATTTT
    TAAGGCATTTGCCTATAACATTTAAGCCTTAATTCATATGGGTTAGACTGTGGTCTCTCAAATCTTGTTC
    CTGACGGGCGGTGGCACCCTCCAGGATAGATGAAAAGTATTTCTGAAGGGAATTAGTAATCTACACTGAT
    TATCTCAACCACATTTATGAGTGAGGTATAATCTTTACAGTCTAAAGGTTTGTGTTGCCCTGGTGTTAAT
    CTAGACTGGGTTACAATACAGGATGGTTACTGACTTATCTATTTCATGATAGGTTAATCAATATGGTACA
    GTCTTCTGCCAAAGAACACCTTGAATTTTAGGAGTTCTGGACTCTCAAACCTAGCTAGCACATTTGCCCT
    CACAATGTGTTCAATGGATCATAACATCAGCTTTCAGCACCTCCAAGACATGTTAAGTCAGACACTCTGT
    TCTGGGCTGAGACCCAACAGTCTCCGTGAACCCAGGTTATACACACACCCAAGCTTAAGAAATGCTGCAT
    TTTGTTGTGAGGGAGAGAATTATTTTAGAATTTGGAGTAATCTCTGCCTAGGTTTAAAAAAACCTAATCC
    ACTACAGGTCCTTGTCAGAACTTGAAAGTTTACCCTGTGTCACCTTTCCCACACCTAAGCTGGTCCTCTA
    GCCTGGGGCATCCCAGTGCCTACTGCACAGCAGGGTGATTCGGATGCCCAGTGACACTGTTCTCGTCCAT
    ACTGGCAGCATGGCAGTACTTTCCCATTCTGTTCAAAGCTCTTTGGGTATTATGTTAATATTACAATCAT
    AACTAAGGAAATATCTGATGACTGTGGACCTGGGAAGCCACGGTCATCTCTAAGTTTATGTTTCTGAACC
    TTAAAAAAAAATAAATAAGATGGGTGTGGTGGCTCTCGCCTTTAATCCCAGCACTCAGGAAGCAGAGGCA
    GGTGGATCCCTGTGAGTTTGGGGTCAGCCTTATCTACATAGAGTTCCAGGACAACGAAGGCTGAAAGTCT
    GTCTTAAGAAGAGGGAGGGAGGAGATGATTGTGGATATCAGGAAGACAGTTCAGCAGGTCACATTACTTC
    TGCACAAGCCTGGTGACCTGGGTTTGAACCCTGAACCCAGAGTGAAAGGAGACAGTCAACTCCCAAAAGT
    TGTCCTCTGACCTCCACACATGCACACATACAATAGTCATACATCAATATATTTTTAAAAAACAAAATGG
    TATCAGGTAGCTGGGCCCTATGATTATTTAAGCTCCAGGTAGCCTCTAGTTCTTTATTTGGAGATGTTTT
    TACCCAAATCTAGAAGAGACCTCCTATAATCCTGGTCCAACAGTGTGAAAAGAAAGGGGAAAGTGAGTAT
    TTGGAAGGCAGATAAGCTGTTGATCGGGTTAATTGGATAACAGTTGACATTCTCTCTCCCATTTCCCCGC
    CTTTTCCTTCTCCTCTGCTCCCCTTCTTCCTCCCTCCTGCCCCTCCTCCACACAGATGTGTCCTCAGAAG
    CTAACCATCTCCTGGTTTGCCATCGTTTTGCTGGTGTCTCCACTCATGGCCATGTGGGAGCTGGAGAAAG
    ACGGTAAGCAGCCTTTTCCCTGTTTTCTTTGGAGCGCCAGCACCTAAACTTGATCTCCAGGCAGCCTGCT
    GGGAAGGGATAGTGAAGTCTGAAGTAGGCAACCGTGCATGGGATTGTCTCAGGAAAAATCCTCAGGGAAG
    ATGGAGGGTTAGCGTCTCTTCCTCCACCTATCTCTCTCCCTTGTTTGGTTTTCATGTTGGATGACAGGAA
    AGGAGTTTCAGGTGAACTATGGTGGTGTGTGGGATATGATCTTGGCCTTAGATACCAGAAAATGACTCTT
    CTAAACAATTGAAATTCCCTAATAGAGAATTTGTAACGTAGCAAGATTGTCTTCTACCCTCTCTTGTCTT
    GCATATTACAACGTGCTCTCAGTGGGCACCATGATGGTTGTCCTCTGGCTATTTTGGGATTGGCATTCAC
    ACTGACTTGGTTTCAGGTCATGGCTCTGCCAGGTATTGATACTGAACCTTTGAGGCTGGGTAGTCTTGTG
    ACCTCAGTTTTCTTTCCTTTTTTTTTCCCCCAGACAGGGTTTCTCTATATAGCCTTGACTTGTCCTGAAA
    CTCACTCTGTAGACCAGGCTGGCCTTAAACTCAGAAATCTGCCTGCCTCTGCCTCCCGAGTGCTGAGATT
    AAAGGAATGCACCACCACTGCCTGGCTGACCTCAGTTTTCTTAATTGGGTGTTCAGTATAGCACAGCCCA
    CATGGACTATTGAATATGTTAATAAATACAGAAAATAACATATGGTAGAGTGAGCAAGCACTATTATTTA
    AAACAAAAAGTCTCTGAACTTTTCATTATCCCAATGGCAATAAGATTATTAGTAGACAAATAATGAAGGC
    ATAGAAGGGGGTGCTCTCTGTCCTCTGTCCTTGTGCCATGTCACAGTTAGCAAATGTCCATCAACTCAGT
    CCTCAGATGGTCGTCACAGGATCTCAAATGTTTCATGGAATACAAGGTAATCAAGTCAGGAATTTCTGCT
    TTTATAGCTACACTTTAGTAATAATTAATATTTATTTAACACTCAATTTCGCAACAACCATGTAAAGTAG
    GAGCTATCTGTATTCCATTTTACAGATAGTAAAAACTGAGGTTCAAGAGGCTAAGTAAGTTTCCCACAAG
    GTCCAGAATAGAACTTGATCCCAGTTTAGTCTCACTTCAGAGCCTGTGTGCTTAAATGCTAGATTTTACA
    AGTAAGGCGTGAAGCAACTATTTCCTGTGGCCATAAAGAGGAAATGGGTGTAAATTATAAATGAAAAGAT
    TTCCCAGCTGCTGATTAGAAATAATCTTCTGAGAATCCAGGAAAAAAAAAAAAGGTGAGCCTTTTTTTCA
    TTCTGAAGAATTACAAGAGTTTGAACGATATTCGCAGGTAAAGGTGCTTGCCACCAAGTCCGATGACCTA
    GACTTCATCACTGCGATCAGCATGATGGAAGGAGAGGATCGAGTTCTCTAAATTGCCCTCTGCTCTCAAG
    ACATGAGCTGTGACACCTGTGCACACACACACACACACACACACACATGAAAGAATTAAATGCAACTTTA
    AAAAGAGTAATCTCCAGCCTGGCCACCCACGCCAACAGAATTTTGGATGCTTTCCTAAGTAAAGCCTTTG
    CCACAGCCAGATGGACAACTTAGGCCCTGGCAGAGTTAACCCCTTAGTTACTTCTGACCTGATAAACCAC
    AGAAATTTCAGAAAAGCAACAGGAGAAACACAGAGAGAGCTTGTGTTGGAGTCACAATGGTGGCCCGAAG
    CCCAGCAGATATCTAAGCTCTGATTCCAACGTGTCCAAGAAGTTCTTTCCCTGCCCTGGGGCTAAGGTCT
    TCTAGGGTTTCTGAGTTCAGGTTCTAAAAGGAAAATAATAGACAGCCTGTTTCATGGCCTAGGGGACAAA
    GGGAAACTGGGAGCCAAAGGGTCCTGTGCACTGGAATCAGCTCAGGCTAACGATTTCTGAAGAGCTTGGC
    CTCAAGTGATTTGTACCTCTTATCTTTGCTCCCCATTCAGCAGTGACAAATCCCACTGATGTAAAAGTAA
    GAGCTTAGCATTTCACAAGGCTTTAAGCTCCAATGCCAATCACAAAGCCACCATTCACAGGGCACTTAAG
    GAAACTGTCTTAAGCTTGCATACAAATTAGTTTAGATGTTACCTTCTAAAGAAAGCACTAATATTTTGCT
    TTCTTTTTTCTTTTTTTAAAAGAATGACATCAGACTAGGGCATGGCAGTTCATGCCTGCAATCCCTGAAC
    TTGATAGATCAAGACAAGAAGAAAGCCCAATTTTGTGGCCAGCCGGGGCTATAAAGTAAAATTCTAAGTA
    GCTGGCTGTCTCTTCCCACCCCCGAGGCCATGGCTCTCTGAAAGCCCTTTGCTGGCTCTGCTCCATAGCC
    GTGCTATTCCAAAACCCAGGCTCTTCACCACTATGCCTTCCATATTCAATCAGGCATCAGATGACTGGGG
    CCATCCTGATCTGACTCAGCAACCAAGAACAAACCTCACTGATGGGTTTTCAGGTGATGCTGATGATGGA
    GGTTGAAAGACGACCCTGCTTTACGGGTCACTGCATTAAGCTAGTCTTTCGATTAATAATTGATATGTAG
    GTAACCTTTGAAGCTTTGTCACATGAGGCTTTGGTGTGACCCACTCCAGAATACCTTATGGAGATATTCT
    GGAGGCTCCTTCAGAAACTAGCATCAGATATCCATTGCACAACACAGGAGACAAAAGATCCGAAAGACAC
    CATTTGTTACCCTTCACACTTGATGGGACCTCACTTAGCCCTTGGCCATCAGCAAAAACAGTTCCTTCAA
    AAACTGAGACAATCATCTACTGCTTTGTAAGAGTGTAAAATAGCTTTTAAAAACAAGTAGTTAATGGCAA
    TCTCCGTCCACAAATTTCATGCAGAAGAGACTTAAAAAAAAAAAAACCTAAATCTTTCTGGATAATTTTA
    AGAGTTTTTCAGTGCATTTGCCTGGCTCTTTGGCCTGTGCATGACAGTGATGATGATGATGGTGGTGGTG
    GTGGTGATGATGATGATTTTAAAAAGCTAGCACAGGGTTATCCTGCCGTAACCTTAGCTGTGCTAAGGTG
    CATGCTTTTTTACCATCTATCCTCACAGCTAATCCTATTAGCATAGACAATATTGTTCTTACCATTTTGT
    GAGGACTCTGGGGCTACTGGTGGTTCAGGACCTTGCTCAAAATCTCCTTTGCAATTAGAGAAGATAGGAT
    TTCTGCATCTTTCTTTCTCCACACCTATGCTTTAAGCCACTATGTTATTCTGCTCAATACTCTGCCCTGT
    GTATTCCCTGTGAATTACAACTGGAAGCTTACTCCCCCTCATCTCCATTCCCAACACACACACACACACA
    CACACACACACACACACACACACACACACTAGTGGCTTCTGACAAGGAATCTTGTGCTGTTTGCATCTTT
    TCTTGTTTCAAGGTCTTCCCTTCCTAATCAGAGACCGATTATTACTGGCCACCTGCCAGTTAAATGACTC
    GGTGGCAGCTATAGAACTGTTTCTCTGTTCTGTCCCCATGCCCTGAGATCTCTCAGCAAGCACTTGCCAA
    ACTCCTGTGAGCTATGACGTTCCAAGGGCCATGGGTGTGTCAAGACACTGAGTGAAACCCAGTTCCCGGG
    GTTTGTGAGGCTCCTGGTCCAGGTCCTCACTGCTGGTGCTCTCCAGTTTATGTTGTAGAGGTGGACTGGA
    CTCCCGATGCCCCTGGAGAAACAGTGAACCTCACCTGTGACACGCCTGAAGAAGATGACATCACCTGGAC
    CTCAGACCAGAGACATGGAGTCATAGGCTCTGGAAAGACCCTGACCATCACTGTCAAAGAGTTTCTAGAT
    GCTGGCCAGTACACCTGCCACAAAGGAGGCGAGACTCTGAGCCACTCACATCTGCTGCTCCACAAGAAGG
    AAAATGGAATTTGGTCCACTGAAATTTTAAAAAGTAATTCCATCCCCCTTGACAGTATCTTTTCTTCTAC
    CCCTCCTTAAAAAGTAATAATTGCAAGTGTGTGTGTGTGTGTGTGTGTGTGCGCGTGCGCGTGCGCGTGC
    ACTGAGGCTCAGAAATGTTTCTCATTTCCTTTGAAACAGGAACTCTCATTGGCCCATAGCTTACCAATAA
    GCTTTCTTGGTTAGCCAATAACATCCAGAGACCTTGTCTCAAAAAAAAAAAAAAAAAAATCTCACCCTTG
    TACCATGTAATGGCCAGTGATCCATCCACAGTAAAAAAGAAATAAATAAATAAAATAAAAATAAAAAATA
    ACAAAAACAAATTTCTTTATTAAAAGAAAACTCCTAAATATTTAATTTTTCTTTTCTTTTCAAATGCAGA
    TTTCAAAAACAAGACTTTCCTGAAGTGTGAAGCACCAAATTACTCCGGACGGTTCACGTGCTCATGGCTG
    GTGCAAAGAAACATGGACTTGAAGTTCAACATCAAGAGCAGTAGCAGGTAAGGGAGCTGCCATGATTTCT
    CAAGGTTTTGTGTGATCTACTTCATAACAAAAAGAAGTACAAACTGCCCATGGATTATCAGTGACTGGTA
    ATGGAACAGCAAAGTCAATTCTGTATTTTTTTTTTCAACTTCCCTCTGCTCGCCTAAGGAAATAGCATGG
    AGCTTTAAGCCAGCCCTCTGAGACTAGTTGGCTGGGTAGTTTGAATTCACTCATCCGAATTGCCTGGCCG
    TGCTTCATTAAGGCTCACAGCACCATGGGGAGGAATTAAAAAGAAGCCACAGCTACAAATAACTGGCCAG
    AGCTCGACCTTGTGTCAAAAGCAGAATTGAGTGCTCCAGGGCCTTGCTGTTGGAAGCCCTGGAACAGATT
    CCAGAACCCTCTGAGGCCTTTCGAGATTTTCCTAGAGCCTTTTCAACTTCTTCCCCCCCCCACCCCCACC
    CCCCAACCGTTATTCTGAAGTTCAGTTTCCCCAATGCACTTGGGCTTTTCCTAAAAACATCATCTCCCAG
    GGCTGCAGAGCGGGTGTGTCAGACTGCTCTACAGCTGACTTTAGTCAGCAACTAAATCCCTGAGCCACAT
    GGAGGGCAGGGAGTGAGCGCCCTGGCTCTCCCACTATTTAAATGAATAAACGGGGGACAGGGGGTGTGGG
    GGGGACGACTCCCTTTTCCATCTTTATCATGAATTTGCTATGACTTAACTTAGCTAAGGAAAATAATGGT
    TCAGGGTGACATCAGAGTCCTTGTTGCTTCTATGAGAATTTTCTGATCTGGTAAAGGTGGCCCCAAAGGA
    GACCAGTGTACAATGCAGATGCTTACATATAGAAACATGCCAGACCTCTATGTGCTTTAAAAAATTAGAC
    AAGCACGATACATTTTCCTGCTAGAGAAATCTTGCACGTGTTCCTGAAGAAACTAGTACATGGATAAGGG
    TCAGTGGAACATTTTGCATAATTAGCAAAAGCATGCTCAAATGGAACATCAATGTGCATCTATTGAAATA
    AATAACTCAACTTTCGTGTTCTGTTTAGAGAACTCCCCCACAATACTTTCAGAGAGGAAAAAGACGTTAC
    AAACTCATGCATATATATTTTTCATTTGTCTTTAAAGTTGTGCTACTCTTTGGATAAACTACATATGTAC
    ATATATGTCTGTGTGCATGTCTATCTTAAATATTCATTTGGAAAAACTCTAGAAGTAACAAACTGTGAAC
    AATGTCTATATGTGGGACTCAGATTTGAGTAAAAAGGAGAATTGTGGCGACACGCATGTTTTCTTTATAA
    TCATCTTTGTTGTTATAATTCTTTATAATTCAGCAGTTTCTCTTGCATTACGATATTTTCAGGTTCATTT
    AGGCAGAGATGCAACTTTCAGTCTCTTACAGCTAAGCTTCCACTCTGCAAAGGGCAAGGGGGTTTTTGTT
    TGGTTTTGGTTTTGGTTGTTTTATTCCACTGGAGAAATCCAACCAGCTGGACAGACTGGCCTCGGACTGT
    AGACACCAGGCACTCTATCTCTGATCTATAATACAGAACTCATTCTGTCTTTGCCATCAGTTCCCCTGAC
    TCTCGGGCAGTGACATGTGGAATGGCGTCTCTGTCTGCAGAGAAGGTCACACTGGACCAAAGGGACTATG
    AGAAGTATTCAGTGTCCTGCCAGGAGGATGTCACCTGCCCAACTGCCGAGGAGACCCTGCCCATTGAACT
    GGCGTTGGAAGCACGGCAGCAGAATAAATATGAGAACTACAGCACCAGCTTCTTCATCAGGGACATCAGT
    GAGTTTTGCCTGGGAGCAGGTGCTAGGGAAGCAAAGACGATGTCTGCCACGTGTCCATGATACCCTGGGG
    CATAGGATTGGCCTGCATTCTTTTCCATTCTCCTAAAAAGCATGATACAGACACTACCCTCTTCATCTTA
    CAGATGAGGAAATTAAAGGGTTAAGCTAACCTTTTTCCAGATCTCAAATCTCACAGACAGCAGACCAGAC
    CCAGACCTGCCCCAATGGTGGCACCTAATGGATTATGGGTTAACTACATGCTTAGTGTAAAGCAGAAACC
    AAGCACAGGGAGGGTTCCCTGATTGCTGAGCCCAGATACGAGACTCTATTAAGCAGAATGACTCAACCAG
    TAGGGACTTCCGGTCTGTGCAGTGCAGAAAGGGTTCTAAAATGTAGGATATGGCCAATAATGGTGACTTC
    CTCGAGTGGAAAGATCACTCTCAGCAGATCCCTCACAGGTGCTGCCCTGATGCTCAGAGAAGTGAAAGAG
    CTATTCCAGAGACTAGAAGGTGGAGGGCTCTGTCAGCCAAGCTCAGGAGCCTAAGTCCTAGCTCCAAAGC
    CTGGCCAGGATAATAAAGGATGCTTGTCTTCTTCTGCAGTCAAACCAGACCCGCCCAAGAACTTGCAGAT
    GAAGCCTTTGAAGAACTCACAGGTGGAGGTCAGCTGGGAGTACCCTGACTCCTGGAGCACTCCCCATTCC
    TACTTCTCCCTCAAGTTCTTTGTTCGAATCCAGCGCAAGAAAGAAAAGATGAAGGAGACAGAGGAGGGGT
    GTAACCAGGTAGGATTTATGCAGCCCATGTTGGGGCCATTGGAAAGATCCTGACAACCAGGTGCTTGCCT
    CAGGACCAGAACTGTGTTAAGGGCTCTCCATGTGAATCACACACAACCTATACAAAGTCCCAAAGGCAGC
    CAGTATTGCTGTCACCTCTCCACAGAGGCCAGGAAAGCTCAGAGAGGCTATGTTTTGCCCACGTTCTTCC
    AGCTAGAGCAGTGGCAGAACCAGTACCCAGCAATGGTTTGATGGAGAAAGAGATGGTTAGCTCAACCACT
    TCAATTCCTCCAGTATAGTCGATTGTCAATCCTCAACCAAAGTTGTTCATGATATAGAAACCTGGGATCC
    AGGAGCCTATAATGATTGTTTAAACTGAGTAAATATAGTAAATGCTAAAACATTGATGCTGGGACCCACC
    AAATAAGGATAATCTCTACTTCCTTAATATGAGCAATCATTTCACCTTACAAGATGTCCACCTTTACTGG
    GCTAATATGGTGACTTTCTACCTTTCGGGTCCTTAGCACAGTGATCTCTTGCTAGGTTTGAGGAACACTT
    TTATGAGCATTTAAGTCACAGAGCAAAGGATTCTTCCAACCTAGCATTGAGAATGTGATAGCAATTCAGC
    TCAGAGATTCAACCACTTCCTAAAAGCAGGAGCAAAAAATGGGATTATTTTGAAACAGATTTTAACTGCT
    ACCTAAGTTTCATCCCTGTGTGTATGTATTTAATATACCAATCTTAAATAGGTAATGTCTAGTGCTTTCT
    ACTTGCTAAAAGCTTTCTACGCATTTTTACATAAATGTTGTATTTAGCTGCTGCAATTCTTAGAGGCAGG
    CATCCTGGGACCTACTCATGAATAAGGAGAGACACAGAGAACCAGAGATATTTTCCTGAAGCACCACAGC
    TGTTTAGTAGCATTGCCAGGATTTGAAGTCACTTTTGTAAGCTGACAGGCATGGTAATTCTTGATGTCAA
    GACAGCTAAAGAAAGCTGGTCTCAGTCCCAACATCAGCTTCATATGGGAGTGGTTACCCATGGGACCTTG
    GGGTAATGCTGCTACTTCAAGAGGCAGAAGGGGGAGCAAGCCATTTCTGTTGGAGTTTACTAAATAGAAA
    AGTGAGAGTGGGGTGGTGGATGAATCATGCTGGAATATGAAGACCTGAATTTTAACCCCCAGAACCTCTG
    TAAAACTGAGTAGTAATGCCTGAACCTGGGCTCTGGAAAGAGGTGGGACACATACACACACACACACACA
    CACACACACACACACACACACACACACACACACCTCTGATTATAGCTTCTACCAGCAGGTGGTGCAAGGG
    GAGCGCCCATGCCAGCCTGCCTTATATTGCCTAAAGCCCTTACTTAAATTTTCCTCTCCTTTGCAGAAAG
    GTGCGTTCCTCGTAGAGAAGACATCTACCGAAGTCCAATGCAAAGGCGGGAATGTCTGCGTGCAAGCTCA
    GGATCGCTATTACAATTCCTCATGCAGCAAGTGGGCATGTGTTCCCTGCAGGTGTGTGAGCAGGACCGTG
    AAGCCCAACCACACCTGCACATTTAGTGGTCCTGGTGGAGGACAACAGGAGGCTTGTGAGGGAGGTGGGG
    GTGGAATTCCTGGCATAGGGATTCCAGTCAGAAACACATTTGTTCATCAGCAGATGTTAACTTGATGTGT
    ATGTTAGGTCAGGCACTATGCTAGGCCAAGGATATTACAATAAAGAAAACAAGCATGGGTCCTACCCTTG
    TACAGGGAAAGGCAAGAAGGACCAGAGGAGGAGGAAATCAGGAGTCAGAGTATGGAGAACTTGGAAGTAG
    ACAGAAGTTGGAACCATATCTTAGGGGTTCTGGGGAACCACTGAAGTCTTTTTAATAAGGAAATATCCCA
    ATCAAAGCCCTTTTAAGAAAAATCTCTCAAGAGAGTCCCTGGCAAGCATGAGACCAGCTGAAGTCCAAGC
    CCTGTAAGGAAGGGTTGGGCTGTCTCGCACAGAAATCCTCAAGACCCAGTGTCTGGGGATAATAATGTGT
    GGTGTGAAATAGGAGGGTATTGAGGCTATACCTCTGACCAATATGGACTTACCTACCCACATTCCTTTAC
    AGGGTCCGATCCTAGGATGCAACGTTGGAAAGGAAAGAAAAGTGGAAGACATTAAGGAAGAAAAATTTAA
    ACTCAGGATGGAAGAGTCCCCCAAAAGCTGTCTTCTGCTTGGTTGGCTTTTTCCAGTTTTCCTAAGTTCA
    TCATGACACCTTTGCTGATTTCTACATGTAAATGTTAAATGCCCGCAGAGCCAGGGAGCTAATGTATGCA
    TAGATATTCTAGCATTCCACTTGGCCTTATGCTGTTGAAATATTTAAGTAATTTATGTATTTATTAATTT
    ATTTCTGCATTTCACATTTGTATACCAAGATGTATTGAATATTTCATGTGCTCGTGGCCTGATCCACTGG
    GACCAGGCCCTATTATGCAAATTGTGAGCTTGTTATCTTCTTCAACAGCTCTTCAATCAGGGCTGCGTAG
    GTACATTAGCTTTTGTGACAACCAATAAGAACATAATATTCTGACACAAGCAGTGTTACATATTTGTGAC
    CAGTAAAGACATAGGTGGTATTTGGAGACATGAAGAAGCTGTAAAGTTGACTCTGAAGAGTTTAGCACTA
    GTTTCAACACCAAGAAAGACTTTTTAGAAGTGATATTGATAAGAAACCAGGGCCTTCTTTAGAAGGGTAC
    CTAAATTTAAAAGAATTTTGAAAGGCTGGGTATCGGTGGTATATGCTTTTAATTCCAGCACTCAGGAGAC
    CAAGGCAGGCAGATCTCTGTGAGTTTGAGGACAGCCTGGTGTACAGAGGGAGTTCCAGCACAGCCAGTGC
    CACACAGAAATTCTGTCTCAAAAAC
  • Protein Sequence : Show Sequence
    >gi|6680397|ref|NP_032378.1| interleukin-12 subunit beta precursor [Mus musculus]
    MCPQKLTISWFAIVLLVSPLMAMWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIG
    SGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCS
    WLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELAL
    EARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEK
    MKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Toxoplasma gondii mic2 mutant vaccine
5. TNF-alpha
  • Gene Name : TNF-alpha
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 21926
  • NCBI Protein GI : 7305585
  • Genbank Accession : AB039224
  • Protein Accession : NP_038721
  • Taxonomy ID : 10090
  • Chromosome No : 17
  • Gene Starting Position : 35199389
  • Gene Ending Position : 35201995
  • Gene Strand (Orientation) : -
  • Protein Name : tumor necrosis factor
  • Protein pI : 4.75
  • Protein Weight : 24010.47
  • Protein Length : 235
  • Protein Note : Also known as DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434
  • DNA Sequence : Show Sequence
    >gi|372099093:35199389-35201995 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J
    CTTTATTTCTCTCAATGACCCGTAGGGCGATTACAGTCACGGCTCCCGTGGGGAGCAGAGGTTCAGTGAT
    GTAGCGACAGCCTGGTCACCAAATCAGCGTTATTAAGACAATTGGGTTAGATAAATATTTTGTTTTAAAC
    ATAAGCAAAAGAGGAGGCAACAAGGTAGAGAGGCCAGGTGGGGACAGCTCAGCTCCGTTTTCACAGAAAA
    CATGTCTGTCTGAAGACAGCTTCCCACACTGGGTCCTCCAGGACACCCCGGCCTTCCAAATAAATACATT
    CATAAGCAAATAAATAAATAATAAATAAATAATAAATAATAAGTGCAAATATAAATAGAGGGGGGCTGGC
    TCTGTGAGGAAGGCTGTGCATTGCACCTCAGGGAAGAATCTGGAAAGGTCTGAAGGTAGGAAGGCCTGAG
    ATCTTATCCAGCCTCATTCTGAGACAGAGGCAACCTGACCACTCTCCCTTTGCAGAACTCAGGAATGGAC
    ATTCGAGGCTCCAGTGAATTCGGAAAGCCCATTTGAGTCCTTGATGGTGGTGCATGAGAGGCCCACAGTC
    CAGGTCACTGTCCCAGCATCTTGTGTTTCTGAGTAGTTGTTGAAAGCTCTGAGCACAGAGTTGGACCCTG
    AGCCATAATCCCCTTTCTAAGTTAGAAGGATACAGACTGGGGGCTCTGAGGAGTAGACAATAAAGGGGTC
    AGAGTAAAGGGGTCAGAGTGGGGGCTGGGTAGAGAATGGATGAACACCCATTCCCTTCACAGAGCAATGA
    CTCCAAAGTAGACCTGCCCGGACTCCGCAAAGTCTAAGTACTTGGGCAGATTGACCTCAGCGCTGAGTTG
    GTCCCCCTTCTCCAGCTGGAAGACTCCTCCCAGGTATATGGGCTCATACCAGGGTTTGAGCTCAGCCCCC
    TCAGGGGTGTCCTTGGGGCAGGGGCTCTTGACGGCAGAGAGGAGGTTGACTTTCTCCTGGTATGAGATAG
    CAAATCGGCTGACGGTGTGGGTGAGGAGCACGTAGTCGGGGCAGCCTTGTCCCTTGAAGAGAACCTGGGA
    GTAGACAAGGTACAACCCATCGGCTGGCACCACTAGTTGGTTGTCTTTGAGATCCATGCCGTTGGCCAGG
    AGGGCGTTGGCGCGCTGGCTCAGCCACTCCAGCTGCTCCTCCACTTGGTGGTTTGCTGAGGGGGGGGGGG
    AGGATTGAGTCAGTGTCACCCTCTTAGTTCACACTCCACATCCTGAGCCTCAGCAGCTACCCACACTTCA
    CTTCCGGTTCCTGCACCCTCTGTCTTTCCACATCCCATTGGCTATGAGGTCCCGGGTGGCCCCCTGATGC
    CTTGCTTTTGAGTCACTGCTCTGACTCTCACGTGCTGTCTCTAAGAGCTCTGTCTTTTCTCAGCCTGGCT
    CGACACCCCTCAACCCGCCCCCCAAAATCATGCCCCTTCATTCTCAAGGCACATGTAAAGAAATCTTACC
    TACGACGTGGGCTACAGGCTTGTCACTCGAATTTTGAGAAGATGATCCTGGAGGGGAAGAGACAAAGGCA
    AGGATGAGCCTTTTAGGCTTCCCAGCAAGCATCTATGCACTTAGACCCCTTTCCTCCCAAACCAAAGCTT
    TAAGTTCTCCCCCCACCCCATCTCATCCCATGCCTAACTGCCCTTCCTCCATCTTAAATTAAGAGAGAGG
    TGTGGGAACACTTACTGAGTGTGAGGGTCTGGGCCATAGAACTGATGAGAGGGAGGCCATTTGGGAACTT
    CTGTGTAGGAAAAGGAGGTTAGTTAAGACAGACTCACCCCAAAGGAGAAGCCTCCCGGCTGATTGCCCCG
    CTTACAGTTCCTCTTTGCCCCACCCCACCCCCCAGCTTTGTGTTTTTCTTCTTCATTCATTCATCTGTCC
    AACCCACGGCTTCTTTCTGCGGTGCCCTCTGTGCTTGATCTCCCGTTATCTCCCCTTCATCTTCCTCCTT
    ATCTCTCATGCCTCTCTCATTTCTGTCTCTGAGTTTTATCTCTTGCTTATCCCCTCTTCCCCTGGCCACA
    TCTTTCCAGATCTCTCCACGTGTGAACACACTTGTTCGTTCATTCATCTCTCTGTGCATCCGACGAAGGA
    TGTTTAGTCAGCTGGACGCATGGGTCCGAGGTCCTGACTCTGTCCCCTCCACACTCTCCTCCACCTTGCC
    CTGCCCATTAGCCCACTTCTTTCCCTCACACTGTCCTTCTTGCCCTCCTAACCCGTTTTGCTTGTGAGCG
    AGAATAAGGGTTGCCCAGACACTCACCTCATCCCTTTGGGGACCGATCACCCCGAAGTTCAGTAGACAGA
    AGAGCGTGGTGGCCCCTGCCACAAGCAGGAATGAGAAGAGGCTGAGACATAGGCACCGCCTGGAGTTCTG
    GAAGCCCCCCATCTTTTGGGGGAGTGCCTCTTCTGCCAGTTCCACGTCGCGGATCATGCTTTCTGTGCTC
    ATGGTGTCTTTTCTGGAGGGAGATGTGGCGCCTTGGGCCAGTGAGTGAAAGGGACAGAACCTGCCTGGTT
    GGCTGCTTGCTTTTCTGGGAGCTATTTCCAAGATGTTCTGGAGTTTCTGTTCTCCCTCCTGGCTAGTCCC
    TTGCTGTCCTCGCTGAG
  • Protein Sequence : Show Sequence
    >gi|7305585|ref|NP_038721.1| tumor necrosis factor [Mus musculus]
    MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRDEKFPNGLPL
    ISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQ
    VLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQL
    SAEVNLPKYLDFAESGQVYFGVIAL
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Toxoplasma gondii mic2 mutant vaccine
IV. Vaccine Information
1. Ovilis Toxovax
a. Tradename:
Ovilis Toxovax
b. Manufacturer:
Intervet
c. Vaccine Ontology ID:
VO_0001138
d. Type:
Live, attenuated vaccine
e. Status:
Clinical trial
f. Host Species for Licensed Use:
Sheep
g. Immunization Route
Intramuscular injection (i.m.)
h. Description
S48 strain with a lost ability to form cysts after passages in mice(Buxton and Innes, 1995)
2. rBCGpMV361-TgCyP
a. Vaccine Ontology ID:
VO_0004656
b. Type:
Recombinant vector vaccine
c. Status:
Research
d. Host Species for Licensed Use:
Baboon
e. Preparation
Following amplification of the T. gondii cyclophilin gene, the shuttle expression plasmid pMV261-TgCyP and integrative expression plasmid pMV361-TgCyP were constructed, and their expression was stimulated after transfection into BCG (Yu et al., 2013).
f. Immunization Route
Intramuscular injection (i.m.)
g. Mouse Response
  • Vaccination Protocol: rBCGpMV261-TgCyP and rBCGpMV361-TgCyP (106 CFU) were used to immunise the mice in two different manners (through an i.v. or oral route) (Yu et al., 2013).
  • Vaccine Immune Response Type: VO_0003057
  • Challenge Protocol: Two weeks after the third vaccination, mice were selected randomly from each group and challenged intraperitoneally (i.p.) with 103 highly virulent T. gondii RH strain tachyzoites (Yu et al., 2013).
  • Efficacy: The levels of Th1-type IFN-γ, IL-2 and IL-12 were significantly increased following immunisation with the rBCG vaccines via the i.v. or oral route, which indicated that catalytic activity against T. gondii infection was generated in the mice. rBCGpMV361-TgCyP i.v. inoculation resulted in a higher protection efficiency, as demonstrated by the increased survival time and survival rate (17%) of BALB/c mice (Yu et al., 2013).
3. T. gondii DNA vaccine encoding MIC8
a. Vaccine Ontology ID:
VO_0004219
b. Type:
DNA vaccine
c. Status:
Research
d. Antigen
MIC8
e. Gene Engineering of MIC8
  • Type: DNA vaccine construction
  • Description: To construct the pVAX-MIC8 expression plasmid, the coding sequence of the T. gondii MIC8 gene was amplified by polymerase chain reaction (PCR) from genomic DNA of T. gondii. The PCR product was cloned in pGEM-T easy vector (Promega, USA) and sequenced in both directions to ensure fidelity and generated pGEM-MIC8. The MIC8 fragment was inserted into the BamH I/Xba I sites of pVAX I by double digestion with BamH I/Xba I restriction enzymes, and ligation was finally done using T4 DNA ligase. The resulting plasmid was named pVAX-MIC8 (Liu et al., 2010).
  • Detailed Gene Information: Click here.
f. Vector:
pVAX1 (Liu et al., 2010)
g. Immunization Route
Intramuscular injection (i.m.)
h. Mouse Response
  • Host Strain: Kunming
  • Vaccination Protocol: Mice (20 per group) received injections of 100 μl of pVAXMIC8 plasmid or empty pVAX I vector into each anterior tibial muscle (final plasmid concentration, 100 μg/100 μl). The injections were repeated using the same protocol 2 and 4 weeks after vaccination. Tail bleeds were performed on vaccinated mice on the day of first vaccination, second vaccination, and third vaccination and pre-challenge to ascertain the presence of specific anti-T. gondii antibodies (Liu et al., 2010).
  • Challenge Protocol: Mice in all groups were intraperitoneally challenged with the virulent T. gondii RH strain (103 tachyzoites per mouse) 2 weeks after the last immunization (Liu et al., 2010).
  • Efficacy: Immunization of mice with pVAXMIC8 dramatically increased the survival time (10.3± 0.9 days) compared with control mice which died within 5 days of challenge (Liu et al., 2010).
4. T. gondii DNA vaccine pcDNA3-MIC3
a. Vaccine Ontology ID:
VO_0011523
b. Type:
DNA vaccine
c. Status:
Research
d. Gene Engineering of MIC3
e. Vector:
pcDNA3 (Xiang et al., 2009)
f. Immunization Route
Footpad injection
g. Mouse Response
  • Host Strain: Kunming
  • Vaccination Protocol: All studies were done with 6–8 weeks old Kunming mice. For experimental group, 100 μg pcDNA3-MIC3 were injected into mouse hind footpad three times, at week 0, 3 and 6, respectively. The mice of control groups were vaccinated with 100 μg pcDNA3 or normal saline (NS) three times (Xiang et al., 2009).
  • Challenge Protocol: Immunized Kunming mice were challenged intraperitoneally with 500 tachyzoites of RH strain T. gondii 9 weeks after the first immunization (Xiang et al., 2009).
  • Efficacy: The survival time of mice in pcDNA3-MIC3 group was significantly longer than those of in group pcDNA3 and NS (Xiang et al., 2009).
5. T. gondii DNA vaccine pcDNA3.1-SAG1-ROP2
a. Vaccine Ontology ID:
VO_0004313
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of p30
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3.1 expressed SAG1 (Xue et al., 2008).
  • Detailed Gene Information: Click here.
f. Gene Engineering of Rop2
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3.1 expressed ROP2 (Xue et al., 2008).
  • Detailed Gene Information: Click here.
g. Vector:
pcDNA3.1 (Xue et al., 2008)
h. Immunization Route
Intramuscular injection (i.m.)
i. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: When challenged with Toxoplasma gondii RH strain, mice immunized with pIL-12 co-administration had significantly higher survival rates compared to co-administration with pCTA2/B or pcDNA3.1-SAG1-ROP2 alone (control) (Xue et al., 2008).
6. T. gondii DNA vaccine pMIC3i encoding the complete MIC3-ORF (RH(ERP) strain)
a. Vaccine Ontology ID:
VO_0004453
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of MIC3
f. Vector:
pCDNA3 (Ismael et al., 2009)
g. Immunization Route
Intramuscular injection (i.m.)
h. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: Mice immunized with pMIC3i displayed significant protection against an oral challenge with 76K T. gondii strain cysts, exhibiting fewer brain cysts than the control mice immunized with the empty plasmid pcDNA3. The co-administration of pGM-CSF enhanced this protection (Ismael et al., 2009).
7. T. gondii DNA vaccine pSAG1-ROP2-SAG2
a. Vaccine Ontology ID:
VO_0004312
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of p30
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3·1 (–) expressed SAG1-ROP2 and SAG2 gene fragments (Cui et al., 2008).
  • Detailed Gene Information: Click here.
f. Gene Engineering of SAG2
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3·1 (–) expressed SAG1-ROP2 and SAG2 gene fragments (Cui et al., 2008).
  • Detailed Gene Information: Click here.
g. Gene Engineering of ROP2
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3·1 (–) expressed SAG1-ROP2 and SAG2 gene fragments (Cui et al., 2008).
  • Detailed Gene Information: Click here.
h. Vector:
pcDNA3.1 (Cui et al., 2008)
i. Immunization Route
Intramuscular injection (i.m.)
j. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: Higher survival rates after lethal challenge were obtained in mice immunized with pSAG1-ROP2-SAG2 as compared to the mice immunized with PBS, expression vector alone or pSAG2, or pSAG1-ROP2. In addition, the protection induced by pSAG1-ROP2-SAG2 was remarkably enhanced by pIL-12 co-administration (Cui et al., 2008).
8. T. gondii DNA vaccine pVAX-TgMIC13
a. Vaccine Ontology ID:
VO_0004528
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of MIC13
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
f. Vector:
pVAX I (Yuan et al., 2013)
g. Immunization Route
Intramuscular injection (i.m.)
h. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: Immunization with pVAX-TgMIC13 induced a strong immune responses demonstrated by significant lymphocyte proliferation, cytokine production and antibody responses (Yuan et al., 2013).
  • Efficacy: Immunized mice showed increased survival time (21.3±11.3 days) and reduced number of cysts in brain of mice (57.14%) after challenge with tachyzoites of the virulent T. gondii RH strain and cysts of the T. gondii PRU strain, respectively (Yuan et al., 2013).
9. T. gondii DNA vaccine pVR1020-GRA1
a. Vaccine Ontology ID:
VO_0004454
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of GRA1
  • Type: DNA vaccine construction
  • Description: Vector VR1020 expressed T. gondii excreted-secreted dense granule proteins GRA1 (Scorza et al., 2003).
  • Detailed Gene Information: Click here.
f. Vector:
VR1020 (Scorza et al., 2003)
g. Immunization Route
Intramuscular injection (i.m.)
h. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: All control pVR1020-vaccinated mice succumbed to infection after challenge, while 75% of the mice vaccinated with pVR1020-GRA1 were protected against infection with strain IPB-G. In the second experiment, all mice vaccinated with GRA1 DNA survived, while 75% of the mice vaccinated with control DNA succumbed to infection (P < 0.05) (Scorza et al., 2003).
10. T. gondii DNA vaccine ROP8-pVAX-1
a. Vaccine Ontology ID:
VO_0004531
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of ROP8
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
f. Vector:
pVAX1 (Parthasarathy et al., 2013)
g. Immunization Route
Intramuscular injection (i.m.)
h. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: splenocytes from mice immunized with ROP8-pVAX-1 secreted significantly high levels of IFN-γ (816 ± 26.3 pg/mL) compared with spleen cells cultured from mice immunized with pVAX-1 (48 ± 10.8) or PBS (45 ± 6.6). Only a low level of IL-4 was detected in the culture supernatant of splenocytes from mice immunized with ROP8-pVAX-1 (148 ± 18.3) but was significantly higher compared with mice immunized with pVAX-1 (50 ± 13.6) or PBS (47 ± 6.1) (Parthasarathy et al., 2013).
  • Efficacy: Mice immunized with ROP8-pVAX-1 DNA had a 100% survival rate until Day 9, whereas all the control mice died. On top of that, mice immunized with ROP8-pVAX-1 DNA showed a significant increase in the survival time (29 days, P < 0.05) (Parthasarathy et al., 2013).
11. T. gondii DNA vaccine T.g.HSP70
a. Vaccine Ontology ID:
VO_0004455
b. Type:
DNA vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of hsp70
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
f. Vector:
pME18100 (Kikumura et al., 2010)
g. Immunization Route
Gene gun
h. Mouse Response
  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: The T.g.HSP70 gene vaccination induced protective immunity against T.g.HSP70-induced anaphylactic reaction not only at the acute phase but also at acute exacerbation of the chronic phase of infection, although the protective effect was partial (Kikumura et al., 2010).
12. T. gondii recombinant vaccine vector rBCGpMV361-TgCyP
a. Vaccine Ontology ID:
VO_0004602
b. Type:
Recombinant vector vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Antigen
TgCyP(Yu et al., 2013)
f. Vector:
BCG
g. Immunization Route
Intravenous injection (i.v.)
h. Mouse Response
  • Host Strain: BALB/c mice
  • Vaccine Immune Response Type: VO_0003057
  • Immune Response: Greater proliferation of CD4+ and CD8+ T cells; levels of Th1-type IFN-γ, IL-2 and IL-12 were significantly increased (Yu et al., 2013)
  • Efficacy: rBCGpMV361-TgCyP i.v. inoculation protected the BALB/c mice, as shown by the 17% increased survival rate compared to those mice not inoculated (Yu et al., 2013)
13. T. gondii Subunit Rop2-Hsp83 Fusion Protein Vaccine
a. Vaccine Ontology ID:
VO_0011521
b. Type:
Subunit vaccine
c. Status:
Research
d. Gene Engineering of Rop2
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
e. Immunization Route
Footpad Injection
f. Mouse Response
  • Host Strain: Balb/c, C57BL/6 and C3H
  • Vaccination Protocol: The immunization doses and boosters of each antigen were the following: Rop2 (44 kDa, 2 μg), LiHsp83 (86 kDa, 6 μg), Rop2-LiHsp83 (not, vert, similar120 kDa, 9 μg) and the mixture of Rop2 and LiHsp83 (2 + 6 μg, respectively). Balb/c, C57BL/6 and C3H mice were immunized by footpad injections on days 0, 21, 35 and 45 with PBS (control) or recombinant proteins (Echeverria et al., 2006).
  • Challenge Protocol: To analyze protection against virulent T. gondii strain (lethal challenge) immunized mice were challenged intraperitoneally with 105 T. gondii RH tachyzoites 2 weeks after the last immunization. To analyze protection against non-lethal challenge, immunized mice were orally infected with 20 cysts of the ME49 T. gondii strain 2 weeks after the last booster. The brain parasite load was evaluated 1 month after infection (Echeverria et al., 2006).
  • Efficacy: Vaccination with fusion protein conferred a remarkable resistance against oral infection with ME49 cysts in C57BL/6 and C3H mice, in comparison to mice immunized with Rop2 alone or the protein mixture. Following lethal challenge, a significant survival rate was observed in Rop2-83 immunized Balb/c and C57BL/6 mice in comparison to control groups (Echeverria et al., 2006).
14. Toxoplasma gondii mic2 mutant vaccine
a. Vaccine Ontology ID:
VO_0003012
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of mic2
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: A mic2 mutant is attenuated in mice (Huynh and Carruthers, 2006).
  • Efficacy: A mic2 mutant induces protection in mice from challenge with wild type T. gondii (Huynh and Carruthers, 2006).
  • Host Gene Response of Ccl2
    • Gene Response: Levels of serum MCP-1 (Ccl2) were higher in mice infected with MIC2 deletion mutants than in uninfected mice on days 4, 6 and 8 post infection (Huynh and Carruthers, 2006).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: Levels of serum IFN-gamma were higher in mice infected with MIC2 deletion mutants than in uninfected mice on days 4, 6 and 8 post-infection (Huynh and Carruthers, 2006).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-6
    • Gene Response: Levels of serum IL-6 were higher in mice infected with MIC2 deletion mutants than in uninfected mice on days 4, 6 and 8 post infection (Huynh and Carruthers, 2006).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Il12b
    • Gene Response: Levels of serum IL-12p40 (IL-12b) were higher than those of uninfected mice on days 4, 6 and 8 post-infection (Huynh and Carruthers, 2006).
    • Detailed Gene Information: Click here.
  • Host Gene Response of TNF-alpha
    • Gene Response: Levels of serum TNF-alpha were higher in mice infected with MIC2 deletion mutants than in uninfected mice on days 4, 6 and 8 post infection (Huynh and Carruthers, 2006).
    • Detailed Gene Information: Click here.
15. Toxoplasma gondii OMPDC mutant vaccine
a. Vaccine Ontology ID:
VO_0003013
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of OMPDC
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: An OMPDC mutant is attenuated in mice (Fox and Bzik, 2010).
  • Efficacy: An OMPDC mutant induces significant protection in mice from challenge with wild type T. gondii (Fox and Bzik, 2010).
16. Toxoplasma gondii OMPDC/UP mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of OMPDC
  • Type: Recombinant protein preparation
  • Description: This OMPDC/UP mutant is from Toxoplasma gondii (Fox and Bzik, 2010).
  • Detailed Gene Information: Click here.
e. Gene Engineering of UP
  • Type: Gene mutation
  • Description: This OMPDC/UP mutant is from Toxoplasma gondii (Fox and Bzik, 2010).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: An OMPDC/UP mutant is attenuated in mice (Fox and Bzik, 2010).
  • Efficacy: An OMPDC/UP mutant induces significant protection in mice from challenge with wild type T. gondii (Fox and Bzik, 2010).
V. References
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