• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: None
• Antigen: PoRV VP7 (Li et al., 2022)
• VP7 gene engineering:
  • Type: Recombinant vector construction
  • Description: (Li et al., 2022)
  • Detailed Gene Information: Click Here.
• Vector: PEDV strain YN150 (Li et al., 2022)
• Preparation: The recombinant virus was constructed by replacing the entire open reading frame 3 (ORF3) in the genome of an attenuated PEDV strain YN150 with the PoRV VP7 gene using reverse genetic systems. (Li et al., 2022)
• Immunization Route: Intramuscular injection (i.m.)
• Description: Recombinant PEDV expressing PoRV VP7 is an effective bivalent vaccine against PEDV and PoRV in pigs. (Li et al., 2022)

Pig Response

• Vaccination Protocol: Ten 10-day-old piglets were purchased from a pig farm and were randomly divided into two groups (Mock-inoculated group and rPEDV-PoRV-VP7-inoculated group), which were housed in separated containments. Prior to immunization, rectal swabs from all piglets were confirmed negative for PEDV, PoRV, PDCoV, and TGEV by RT-PCR. Neutralizing antibodies against PEDV and PoRV were not detected in the sera of all piglets. After acclimation for 24 h, rPEDV-PoRV-VP7 was inoculated to the vaccinated group at a titer of 5 × 10^6 TCID50/mL (2 mL oral administration and 2 mL intramuscular administration per piglet), while mock-infected piglets were inoculated with the same volume of DMEM medium as a negative control. (Li et al., 2022)
• Immune Response: The levels of anti-PoRV VP7 and anti-PEDV S IgA antibodies in saliva from rPEDV-PoRV-VP7-inoculated piglets were significantly increased from 14 to 28 days post-inoculation (DPI) compared with mock-inoculated piglets. These results suggest that rPEDV-PoRV-VP7 could effectively induce the specific IgA antibodies against PoRV and PEDV in piglets. Neutralization antibody titers against PoRV were initially detected at 7 DPI and elevated to about 1:97 at 21 DPI, while neutralization antibody titers against PEDV reached 1:8 at 14 DPI and dropped to approximate 1:2 at 28 DPI. (Li et al., 2022)
• Side Effects: After inoculation, no piglet displayed clinical symptoms such as vomiting, diarrhea, or anorexia. (Li et al., 2022)