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Vaccine Detail

S. pneumoniae SrtA Protein Vaccine
Vaccine Information
  • Vaccine Name: S. pneumoniae SrtA Protein Vaccine
  • Target Pathogen: Streptococcus pneumoniae
  • Target Disease: Pneumonia
  • Vaccine Ontology ID: VO_0004008
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: Recombinant sortase A (SrtA) (Gianfaldoni et al., 2009).
  • SrtA gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant: Freunds emulsified oil adjuvant
    • Adjuvant name: Freunds emulsified oil adjuvant
    • VO adjuvant ID: VO_0000133
  • Immunization Route: Intraperitoneal injection (i.p.)
Host Response

Mouse Response

  • Host Strain: BALB/c mice
  • Vaccination Protocol: Specific-pathogen-free BALB/c mice (Charles River) received three intraperitoneal administrations, 2 weeks apart, of 20 μg of SrtA along with Freund's adjuvant. Controls received the same course of saline plus adjuvant (Gianfaldoni et al., 2009).
  • Challenge Protocol: Three weeks after the last immunization, the animals were challenged intraperitoneally with one of the following S. pneumoniae strains: TIGR4, 1.4 × 102 CFU/mouse; D39, 103 CFU/mouse; or 35B-SME15, 7 × 103 CFU/mouse (Gianfaldoni et al., 2009).
  • Efficacy: Intraperitoneal immunization with recombinant SrtA was found to have conferred to mice protection against S. pneumoniae intraperitoneal challenge and the passive transfer of immune serum before intraperitoneal challenge was also protective. Moreover, by using the intranasal challenge model, significant reduction of bacteremia when mice were intraperitoneally immunized with SrtA was observed, while a moderate decrease of lung infection was achieved by intranasal immunization, even though no influence on nasopharynx colonization was seen (Gianfaldoni et al., 2009).
References
Gianfaldoni et al., 2009: Gianfaldoni C, Maccari S, Pancotto L, Rossi G, Hilleringmann M, Pansegrau W, Sinisi A, Moschioni M, Masignani V, Rappuoli R, Del Giudice G, Ruggiero P. Sortase A confers protection against Streptococcus pneumoniae in mice. Infection and immunity. 2009; 77(7); 2957-2961. [PubMed: 19433540].