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Vaccine Detail

M. tuberculosis Mtb72F Protein Subunit Vaccine
Vaccine Information
  • Vaccine Name: M. tuberculosis Mtb72F Protein Subunit Vaccine
  • Target Pathogen: Mycobacterium tuberculosis
  • Target Disease: Tuberculosis
  • Vaccine Ontology ID: VO_0011412
  • Type: Subunit vaccine
  • Status: Research
  • Mtb72F gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant: AS02 vaccine adjuvant
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Host Strain: C57/BL6
  • Vaccination Protocol: Mice were immunized three times (3 wk apart) with 8 µg of rMtb72F formulated with the adjuvants AS02A. For protein formulations, the required immunization dose of rMtb72F (typically 8 µg) was brought up to either 50 or 43 µl with 1x PBS (pH 6.8) and mixed with 57 µl of AS02A. Mice were injected with a total volume of 100 µl/mouse via the i.m. (tibialis) route with 50 µl/leg. Positive control mice were immunized with BCG (5 x 104 CFU) in the base of the tail (once), and negative control animals were injected with saline, adjuvant alone, or DNA vector (Skeiky et al., 2004).
  • Challenge Protocol: Thirty days after the last immunization, mice were challenged by low dose aerosol exposure with M. tuberculosis H37Rv strain (Skeiky et al., 2004).
  • Efficacy: Immunization of mice with Mtb72F protein formulated in the adjuvant AS02A resulted in the elicitation of a moderate IFN-gamma response and a weak CD8(+) T cell response to Mtb32c. Mtb72F immunization resulted in the protection of C57BL/6 mice against aerosol challenge with a virulent strain of M. tuberculosis(Skeiky et al., 2004).
  • Information about this animal model: Mouse Model for TB research
References
Skeiky et al., 2004: Skeiky YA, Alderson MR, Ovendale PJ, Guderian JA, Brandt L, Dillon DC, Campos-Neto A, Lobet Y, Dalemans W, Orme IM, Reed SG. Differential immune responses and protective efficacy induced by components of a tuberculosis polyprotein vaccine, Mtb72F, delivered as naked DNA or recombinant protein. Journal of immunology (Baltimore, Md. : 1950). 2004 Jun 15; 172(12); 7618-28. [PubMed: 15187142].