• Vaccine Name: M. tuberculosis Mtb72F Protein Subunit Vaccine
• Target Pathogen: Mycobacterium tuberculosis
• Target Disease: Tuberculosis
• Vaccine Ontology ID: VO_0011412
• Type: Subunit vaccine
• Status: Research
• Mtb72F gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001264
  • Description: AS02A (Skeiky et al., 2004).
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Host Strain: C57/BL6
• Vaccination Protocol: Mice were immunized three times (3 wk apart) with 8 µg of rMtb72F formulated with the adjuvants AS02A. For protein formulations, the required immunization dose of rMtb72F (typically 8 µg) was brought up to either 50 or 43 µl with 1x PBS (pH 6.8) and mixed with 57 µl of AS02A. Mice were injected with a total volume of 100 µl/mouse via the i.m. (tibialis) route with 50 µl/leg. Positive control mice were immunized with BCG (5 x 10^4 CFU) in the base of the tail (once), and negative control animals were injected with saline, adjuvant alone, or DNA vector (Skeiky et al., 2004).
• Challenge Protocol: Thirty days after the last immunization, mice were challenged by low dose aerosol exposure with M. tuberculosis H37Rv strain (Skeiky et al., 2004).
• Efficacy: Immunization of mice with Mtb72F protein formulated in the adjuvant AS02A resulted in the elicitation of a moderate IFN-gamma response and a weak CD8(+) T cell response to Mtb32c. Mtb72F immunization resulted in the protection of C57BL/6 mice against aerosol challenge with a virulent strain of M. tuberculosis(Skeiky et al., 2004).
• Information about this animal model: Mouse Model for TB research