• Vaccine Name: Influenza virus DNA vaccine encoding HA from Influenza A virus (A/environment/Hong Kong/156/1997(H5N1))
• Target Pathogen: Influenza virus
• Target Disease: Influenza (flu)
• Vaccine Ontology ID: VO_0004016
• Type: DNA vaccine
• Status: Research
• Antigen: Influenza A virus (A/environment/Hong Kong/156/1997(H5N1)) HA hemagglutinin
• HA gene engineering:
  • Type: DNA vaccine construction
  • Description: A full-length cDNA copy of the HA gene of HK97 was cloned into the EcoRI and BglII sites of pCAGGS/MCS, a vector that contains a chicken β-actin promoter. This construct was designated pHKHA. Plasmids were grown in HB101 bacteria and purified on purification columns (Qiagen, Inc., Valencia, Calif.) (Kodihalli et al., 1999).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005001
• Immunization Route: Gene gun

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Six- to seven-week-old BALB/c mice (n = 50) were inoculated via gene gun with 1 μg of either pTyIrHA or pHKHA affixed to gold particles and were given boosters of the same dose 4 weeks later. Sixty-four control mice were left untreated (Kodihalli et al., 1999).
• Challenge Protocol: Ten days after receiving the boosters, the mice were challenged with 10 50% lethal doses (LD50) of either CkHK97 or HK97 in 100-μl volumes, intranasally. The mice were monitored daily for weight loss, clinical signs, and mortality, and samples were taken from three or four from each group on day 5 postinfection for virus replication (Kodihalli et al., 1999).
• Efficacy: Researchers report that a DNA vaccine encoding hemagglutinin from the index human influenza isolate A/HK/156/97 provides immunity against H5N1 infection of mice. Gene gun immunization of 12 mice with 1 μg of pHKHA provided 100% protection against death from homologous challenge with HK97 virus (Kodihalli et al., 1999).