VIOLIN: Vaccine Investigation and Online Information Network

P97

General Information

Protegen ID

1062

Sequence Strain (Species/Organism)

Mycoplasma hyopneumoniae 232

Taxonomy ID

295358

Molecule Role

Protective antigen

Molecule Role Annotation

a P97 recombinant replication-defective adenovirus (rAdP97c) subunit vaccine efficiency was evaluated in pigs. The rAdP97c vaccine was found to induce both strong P97 specific humoral and cellular immune responses. The rAdP97c vaccinated pigs developed a lower amount of macroscopic lung lesions (18.5 + or - 9.6%) compared to the unvaccinated and challenged animals (45.8 + or - 11.5%). rAdP97c vaccine reduced significantly the severity of inflammatory response and the amount of M. hyopneumoniae in the respiratory tract. The challenge was performed at day 28 after the first vaccination with 106 CCU of the 232 M. hyopneumoniae strain by intratracheal route (Okamba et al., 2010).

Related Vaccines(s)

rAd-P97c

References

Okamba et al., 2010: Okamba FR, Arella M, Music N, Jia JJ, Gottschalk M, Gagnon CA. Potential use of a recombinant replication-defective adenovirus vector carrying the C-terminal portion of the P97 adhesin protein as a vaccine against Mycoplasma hyopneumoniae in swine. Vaccine. 2010; 28(30); 4802-4809. [PubMed: 20472025].

Gene Information

Gene Name

P97

NCBI Gene ID

3105444

Genbank Accession

AE017332

Locus Tag

mhp183

Gene Starting Position

224079

Gene Ending Position

227405

DNA Sequence

>NC_006360.1:224079-227405 Mycoplasma hyopneumoniae 232, complete genome
TTTATTTAGATTCTGGTTCCTGATTATTATTACTTTGTTTTACTGTTATTTTAACTTTTAGACTTGATTT
TTTAGGATCACCGGATTTTGAATCATCCCTTAGGATAAAGTATAGCAATTTAGCATCTCCAGCGATATTA
TCCTCGATTAGTTCAACCTCTGTTTTTCAATTTTTACCTTGTTTTTTAATGATTTCGTCAATTTTTTTAC
CTAAGTCAGGAAGGTAATTAGTTAATTCGGTAGTTGGGCTTTGTTGACTAGGTGCTCCCTCTGCTTTTTT
CCCTTGGTTAGGAGTTCCTTCGGCTTTTTTACCTTGGTTAGGCGCGCCTTCTGCTTTTTTACCTTGGTTA
GGAGTTCCTTCGCTTTTTTTACCTTGATTTGAAGATCCTTCGGTCATCATTGGGTGGCTAAGTTTCTGAT
ATTGGTCACTAGTTACCTTGACTTCCTGGAATTGATATTGAAGATTTAAAACAGTTTTATCTAGTTCCTT
TATTATTTCTTGTTCTTCATACTCGCTTTGATGAACTAGTTCTAAAAATACATTAATTTCCGGTGTTTTT
AGGCTTAATTTATTTTCGTCAGTATATTCTAATTTATAACTAAAAGCCATTGGGAAATAGTCTTCTTTTG
GTTTATTTGTAAGTGAAAAGCCAGTATTAGTAGCAACTGGTTTTGCTGCTTCTGGTTTAGCCGCTACTGG
TTTTGCTGCTTCTGGTTTAGCCGCTACTGGTTTTGCTGCTTCTGGTTTAGCCGCTACTGGTTTTGCTGCT
TCTGGTTTAGCCGCTACTGGTTTTGCTGCTTCAGGTTTAGCTGCTTCAGGTTTAGCTGCTACTGGTTTTG
TTGTTTCTGGTTTAGCCGCTACTGGTTTTGCTGCTTCTGGTTTTGCTGCTGGGGGCTGAGGCAATATACC
TTTTATTTTATTATCCAATTCCTTAACTTTTTTATCTACTTCTTCTCTTTTACCTTCTTTTGTAGTTTCC
CCTTTTTTGATAGCTTCAAATGAATACTGAAGATTATCGTCTAATTTTGCTCAAGGAGCAAAATTATTAA
ATTGGGCTGCTTTAAGGAAAAATGCTTTTAAAAAATCACCGAAGGTTTTAAAATCAGTCCCTTCAAGTAA
ATTTTTATCTAAAATAGTAGTTTTAAAAGCCGAGTTCTCAATTAAAGAATACTTTTTAAGCGCTTCAAAA
ATTTGCGCCTCATTTTTGCCTTCTAATTCAGTTTTTACACTTTGGGGCAATAATAAAACTCCGTAAAATC
CATCAGATTTTATTACATCGTTACGGGCTAAAATATTAAAGGAAAGTTGGTGAATTTCTTTTATCTGATT
GTAAGGATCCTTTTTCTTCAATTCCAAAAATTTTAATTTATCTGCTTCTGCAAAAATATCTTTTGTATAT
TGGAAAACCCCTTTTGGGAATTGTGCTCTGTTAGTTTCTAGTCCCCATTTTTTTGCTAGATCATAAAGAG
TCTCTAAAATTGTTAATTTGTCCTTGGTGAGTAAATCCTGGAAATAACTTGCAACTTGATGTCCATTTTT
AAAAAAGTTATTACTTTTAAGACTTGAAATTACTTGTTCTTTTGTTGTTTGTGGTTGATTTAAAACTGAA
TTAGAACCAATTTTAACTTGTTCTAAATGTCTTGTAAATCTTTGGATATCAGCTTTTTCAGGATGCTGAA
TTTTTCCTGTTCAGGAATTAAGCACGGATTTAAAGGCCCCGAATTCATAGGCATAATTTCCATCAAGTGC
TTGATAGATTTCTTGGTTTTTATTCCCTTTAAAAAGAGCTTCAACTTCAACTTTGGCAAGTTGAGTATTA
TATGGATTTAAGATATTACCATAGTCTAATTTTGCTTGTTCTTCTTTACCTTTTTCAAAAAATGGTAAAT
ATTCGGTTTTAAAAATTTGCTGATTGATATTAGGGAGATCAATTTTTGAGCCTTTTTTAAATCCAGTTAA
TTTAAGAATCCCTTCTTTTACGAGAATATTATCTTTTGTATCTGGATATAAATCATCGGCAAAAAATTTA
TCTTTAATTTCAAGGCGGTAGGGAATTAAAACTATTGATTTATCTTTTTTATCAAGACTTGCGCTTGCTG
CATCTAGGCTATATTCTAAATTTGGCGAGTTAAGACGGTCGTTGAATTTTCCATTGTATTTACCAAAGTC
ATAAATATTTTCTTTTTTATTTAAAAGATTACTAATTTCTGCCTTGGTATGACTGGATCTCTTGTCTCCA
GGGAAAAATCCAGATTTAATATCTGCTAGAAATTCATAGGCAGAAAGATTTTGACCCTTTGCATTTAAAT
CTTCTTTTTGCATATTTTTCAGTGTTAAATTATTAACAAAATCCTCAAAATTTAGAAAATAAGAAGAATT
ATCTTTTTTCATTAATCTTAAAATGTTAATAACTGGCTGAACTTCATCTGGGCTAGCTAAAATTTCACGG
GCACTTTGACTAAGATCTGCTTTTAAAAACAGCGAAGGAATTTGGTTCTGGATACTTACATATTGATTAG
TTGAACTATCTTTTGCAAAGCTAAATTCAAAAATATTACCCAAATTATTAGGTAATTTATTCTCAGGAGC
ATTATTTAGTCTGTTTATTAAATTTTTAAGTACTGGAAAATAATTAGCAAGCTTTATATCTAAATCCTCA
GGATTTCGCGCTGTATTTAAATCGTATTGGAAGTCAATAGCTCTTAGAGTTGAGGGATCTTTTTGGCTGC
TTGTTTTTTCATCAGCAAAATTTGTAATTTTTGTTGATAAATTTTCAATTTGTTGATTTAATTTTTCGGT
AATTTTTTTAAGCGAAAAATTAAATTCGGCAACTAAAAGATTTGACTGTTTGGCAAAAGCAACTGTTTGC
TCATAAATATCAGATTTGGCAATATCACCATTATGAAGTTTTTGTAATGCCTGAAATTTTACCTTAAAAT
TTTGATTGACATCATCAGGGATAATTTCAAGAATCTCAAAAGTTATCTTCGCCTTGGTATATTCAGGATC
TTGAAAATTAATTTTTTCTAACTGATCACCGTTTTTAGTAAAAAAGGAAAAGGAATCAATAACTTTTTCT
TTATTTCTAATATTGTTATTAGAATCAACTAGTCACCTTTTGACTATTTTATAATCAGAATCAGTCTCAA
AAGCATAAGGACTAAAAGCTAATGTTGAAACTTTTGCGGCAAAATCATTTGCAATCTTTCGTGGACTTTC
TGATCTGTATTTTGCCAAGCTGCTAAGTCCGACAGTTAGACCAAAAACTCCAAGACCAACAATTCCGGCA
GTCAAACCAATTTTAAATGTTTTTGATTTTTTACTCA

Protein Information

Protein Name

protein p97; cilium adhesin

NCBI Protein GI

54020389

Protein Accession

YP_115696

Protein pI

9.5

Protein Weight

120335.63

Protein Length

1108

Protein Sequence

>YP_115696.1 protein p97; cilium adhesin [Mycoplasma hyopneumoniae 232]
MSKKSKTFKIGLTAGIVGLGVFGLTVGLSSLAKYRSESPRKIANDFAAKVSTLAFSPYAFETDSDYKIVK
RWLVDSNNNIRNKEKVIDSFSFFTKNGDQLEKINFQDPEYTKAKITFEILEIIPDDVNQNFKVKFQALQK
LHNGDIAKSDIYEQTVAFAKQSNLLVAEFNFSLKKITEKLNQQIENLSTKITNFADEKTSSQKDPSTLRA
IDFQYDLNTARNPEDLDIKLANYFPVLKNLINRLNNAPENKLPNNLGNIFEFSFAKDSSTNQYVSIQNQI
PSLFLKADLSQSAREILASPDEVQPVINILRLMKKDNSSYFLNFEDFVNNLTLKNMQKEDLNAKGQNLSA
YEFLADIKSGFFPGDKRSSHTKAEISNLLNKKENIYDFGKYNGKFNDRLNSPNLEYSLDAASASLDKKDK
SIVLIPYRLEIKDKFFADDLYPDTKDNILVKEGILKLTGFKKGSKIDLPNINQQIFKTEYLPFFEKGKEE
QAKLDYGNILNPYNTQLAKVEVEALFKGNKNQEIYQALDGNYAYEFGAFKSVLNSWTGKIQHPEKADIQR
FTRHLEQVKIGSNSVLNQPQTTKEQVISSLKSNNFFKNGHQVASYFQDLLTKDKLTILETLYDLAKKWGL
ETNRAQFPKGVFQYTKDIFAEADKLKFLELKKKDPYNQIKEIHQLSFNILARNDVIKSDGFYGVLLLPQS
VKTELEGKNEAQIFEALKKYSLIENSAFKTTILDKNLLEGTDFKTFGDFLKAFFLKAAQFNNFAPWAKLD
DNLQYSFEAIKKGETTKEGKREEVDKKVKELDNKIKGILPQPPAAKPEAAKPVAAKPETTKPVAAKPEAA
KPEAAKPVAAKPEAAKPVAAKPEAAKPVAAKPEAAKPVAAKPEAAKPVATNTGFSLTNKPKEDYFPMAFS
YKLEYTDENKLSLKTPEINVFLELVHQSEYEEQEIIKELDKTVLNLQYQFQEVKVTSDQYQKLSHPMMTE
GSSNQGKKSEGTPNQGKKAEGAPNQGKKAEGTPNQGKKAEGAPSQQSPTTELTNYLPDLGKKIDEIIKKQ
GKNWKTEVELIEDNIAGDAKLLYFILRDDSKSGDPKKSSLKVKITVKQSNNNQEPESK

Epitope Information

IEDB Linear Epitope