late transcription factor VLTF-4; initiates transcription from late gene promoters; inhibition of DNA replication causes VLTF-4 to be diffusely distributed in the cytoplasm but is discretely localized during a productive infection; The poxviridae are enveloped unsegmented dsDNA viruses; unlike many dsDNA viruses that replicate in the host nucleus poxviruses encode their own replication machinery and therefore replicate in the cytoplasm; viral genes are expressed in a bi-phasic manner with early genes encoding non-structural proteins involved in genome replication and late genes encoding the viral structural proteins
