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S nucleocapsid protein from Dobrava-Belgrade virus

General Information

Protegen ID

146

Sequence Strain (Species/Organism)

Dobrava-Belgrade orthohantavirus

VO ID

VO_0010960

Taxonomy ID

1980467

Other Database IDs

GI:63030019; Accession:AY961618.1

Molecule Role

Protective antigen

Molecule Role Annotation

Study compared the immunogenicity and protective efficacy of recombinant DOBV nucleocapsid protein (rDOBV N, S) given with Alum or Freund's as adjuvant, or PBS, in C57/BL6 mice. Mice receiving rDOBV N with Freund's adjuvant were protected from challenge (75% protected) (Klingstrom et al., 2004).

Related Vaccines(s)

Dobrava-Belgrade virus S nucleocapsid protein vaccine , Recombinant DOBV nucleocapsid protein (rDOBV N)

References

Klingstrom et al., 2004: Klingstrom J, Maljkovic I, Zuber B, Rollman E, Kjerrstrom A, Lundkvist A. Vaccination of C57/BL6 mice with Dobrava hantavirus nucleocapsid protein in Freund's adjuvant induced partial protection against challenge. Vaccine. 2004 Sep 28; 22(29-30); 4029-34. [PubMed: 15364453].

Gene Information

Gene Name

S nucleocapsid protein from Dobrava-Belgrade virus

NCBI Gene ID

2656266

Genbank Accession

AJ410615

Locus Tag

DOBVsSp1

Gene Starting Position

Gene Ending Position

1324

DNA Sequence

>NC_005233.1:35-1324 Dobrava virus complete S segment gene for nucleocapsid protein, strain DOBV/Ano-Poroia/Afl9/1999
GATGGCAACATTAGAGGAACTCCAAAAAGAAATCAACAACCACGAGGGCCAATTGGTGATAGCCAGGCAG
AAGGTGAAAGATGCAGAAAAGCAGTATGAAAAGGATCCTGACGACCTGAATAAAAGGGCATTGAGTGACC
GGGAAAGCATTGCACAATCAATTCAGGGAAAAATAGATGAATTAAGGAGACAGCTGGCTGATCGTGTGGC
AGCAGGGAAAAACATTGGCAAAGAGAGGGACCCAACTGGACTAGACCCTGGAGATCACCTCAAAGAGAAG
TCAATGCTCAGTTATGGAAATGTCATTGACCTCAACCATCTTGACATTGATGAACCTACAGGGCAAACCG
CAGACTGGCTGAGCATTGTGGTCTACCTGACATCATTCGTGGTTCCAATATTGTTGAAGGCTCTTTACAT
GCTTACCACCAGAGGGAGACAAACTACTAAAGACAATAAGGGAATGAGAATTCGATTTAAGGATGACAGC
TCTTTTGAAGATGTGAATGGGATTAGAAAGCCAAAGCACCTGTTCTTGTCAATGCCCAATGCACAATCTA
GCATGAAGGCAGATGAGATTACACCAGGTCGGTTCAGGACTGCAATTTGTGGACTATACCCAGCCCAGGT
GAAGGCAAGGAACTTAATCAGTCCTGTGATGAGTGTGATTGGGTTTTTAGCCCTTGCAAAGAACTGGACA
GAGCGGGTTGAGGAATGGCTTGACCTCCCGTGCAAGCTACTATCTGAGCCATCTCCAACGTCTTTGACCA
AAGGCCCATCCACCAATCGTGACTACTTGAATCAAAGACAAGGAGCGCTTGCAAAAATGGAAACAAAAGA
AGCTCAGGCTGTGAGGAAACATGCCATAGATGCTGGCTGCAACCTTATCGACCATATAGACTCACCATCA
TCAATCTGGGTCTTTGCAGGAGCACCCGACAGATGCCCTCCTACCTGCCTGTTCATTGCAGGCATGGCAG
AGCTAGGTGCATTCTTTGCTGTTCTCCAGGATATGAGGAACACCATCATGGCGTCAAAAACTATCGGAAC
ATCTGAGGAGAAGCTAAAGAAGAAATCATCTTTTTACCAATCTTACCTACGGAGGACACAATCTATGGGG
ATACAACTGGACCAGCGCATCATCGTGCTCTTCATGGTTGACTGGGGAAAAGAGGCAGTTGATAGTTTTC
ATCTTGGTGATGATATGGATCCTGAGCTCCGGGGCCTGGCACAGGCATTAATTGATCAAAAAGTGAAGGA
AATATCTAATCAGGAACCGCTTAAGCTTTA

Protein Information

Protein Name

nucleocapsid protein

NCBI Protein GI

38505524

Protein Accession

NP_942553

Protein pI

7.53

Protein Weight

46162.49

Protein Length

429

Protein Sequence

>NP_942553.1 nucleocapsid protein [Dobrava-Belgrade orthohantavirus]
MATLEELQKEINNHEGQLVIARQKVKDAEKQYEKDPDDLNKRALSDRESIAQSIQGKIDELRRQLADRVA
AGKNIGKERDPTGLDPGDHLKEKSMLSYGNVIDLNHLDIDEPTGQTADWLSIVVYLTSFVVPILLKALYM
LTTRGRQTTKDNKGMRIRFKDDSSFEDVNGIRKPKHLFLSMPNAQSSMKADEITPGRFRTAICGLYPAQV
KARNLISPVMSVIGFLALAKNWTERVEEWLDLPCKLLSEPSPTSLTKGPSTNRDYLNQRQGALAKMETKE
AQAVRKHAIDAGCNLIDHIDSPSSIWVFAGAPDRCPPTCLFIAGMAELGAFFAVLQDMRNTIMASKTIGT
SEEKLKKKSSFYQSYLRRTQSMGIQLDQRIIVLFMVDWGKEAVDSFHLGDDMDPELRGLAQALIDQKVKE
ISNQEPLKL

Epitope Information

IEDB Linear Epitope