VIOLIN: Vaccine Investigation and Online Information Network

iutA

General Information

Protegen ID

280

Sequence Strain (Species/Organism)

Escherichia coli CFT073

VO ID

VO_0010986

Taxonomy ID

562

Other Database IDs

CDD:273805

Molecule Role

Protective antigen

Molecule Role Annotation

Immunization with IutA protects against urinary tract colinization by E.coli CFT073 in the bladders of CBA/J mice (Alteri et al., 2009).

We demonstrated for IutA, antigen-specific serum IgG represents a strong correlate of protection in vaccinated mice (Mobley and Alteri, 2015).

COG

COG1629P, under P: Inorganic ion transport and metabolism

Related Vaccines(s)

E. coli IutA protein vaccine

References

Alteri et al., 2009: Alteri CJ, Hagan EC, Sivick KE, Smith SN, Mobley HL. Mucosal immunization with iron receptor antigens protects against urinary tract infection. PLoS pathogens. 2009; 5(9); e1000586. [PubMed: 19806177].

Mobley and Alteri, 2015: Mobley HL, Alteri CJ. Development of a Vaccine against Escherichia coli Urinary Tract Infections. Pathogens (Basel, Switzerland). 2015; 5(1); . [PubMed: 26729174].

Gene Information

Gene Name

iutA

NCBI Gene ID

1039578

Genbank Accession

AE014075

Locus Tag

c3623

Gene Starting Position

3461192

Gene Ending Position

3463471

DNA Sequence

>NC_004431.1:3461192-3463471 Escherichia coli CFT073, complete genome
GTCAGAACAGCACTGAGTAGTTCAGACCAAAGGTTCGGCCCCGGCCTTTGTAGTCGTACAGTGAAGCAGG
GCCGTAACCCGGGCTGTAGTACAGAGGTGCACGCTGTCCCCAGACAGTGGTATAGTCACGGTCGAAGAGG
TTCTCAACGCTGAAGCTGAGTGTTCCCACCGGAAGCTGCCAACTACTGATAAAATCGACGGTAGTGTAAC
CATTAATATCGTTACCCTCTGCATCGCTTACGTCGAAAGAAGTGGTGCTCTGTACACGCAGACTCCACGG
TTCCGGCGCCCAGTTAATGTAAGCTGTCGCTTTCGATGGACTTGATTCCTTCACGTCATATTTTTGCCAT
TGACCGTTCACTTTCGACTCGGTTTTCAGCACATTGAAGTTCACACCGGTACTCCAGTCAGTATCCGGGA
TCAGGTAGTCCACCGCACCTTCCACGCCGTAAATACGGCGCCTGTCGTCCTTCACACTGATGGTCAGATC
TTTATTCCTTTCCACGCTCTTATTGGAAAGCGAGTAATATGCCGCGATTTGAGTCCGCAGGTTGTCACCG
GTAAAGCGCCAGCCCAGTTCATAAGAATCGACTTTCACGCCTTCCAGCTTACTGTCGCTGACGTTCACGC
TCTTTGTCAGGGGAAGATGGCCGTTCACTGCTGCACCATAGATGCCGCGACCATAATATTTCCCCGGATC
CGGCAATGCCACCCCCTGGGAAAAATTGAACCATGCCTGCTGACGTTCGGTGATGTGCATCAGCAGACCA
GCATTGAACAGAAAGTTATCGTAATCTACCGAACCACCAGGAATGGCGTCGGCAGATATCGCCTTCCCGG
CAGCAATCTTCTGTTGCTGCGTGTAGTCGATGAAATCATCTACCCTGTTCTCAGTATACTGATAGCGTAC
GCCACCGCTAACGGTAAAAATATCATTAATGTCATAGCTGGATTGCAGGAAGGCCGCCAGATTGGTGATG
TCATATGACGGATAGCGCCCGGTGGTGTAAATCTTATGGTTGTTCAGCCCTCCGGAAGCACTTGCCTGAG
CCAGATCGAAGAACATCTGGTTGGAGGTAAAGCGCTCATGCTCAGCATCCAGCCCCCAGGTGATTTGCCA
GCCGTCCATAAGTTGGCTGTTCAGAGTCAGTTTCATGCCGTACTGGTCGGTATCCTGCTGTGACGAGGAG
AAAGCCGTCGCCTGTTTATTCGCATTTACCGTCGGGAACGGGTAGAACCGCAACGACTCATCGCGGTAGT
AAACCTGACCGACCAGTTCCTGTCTCAGGAAATCACTGTCAGAGTACTGCAGGCTGATCAAATGCCGCTC
AGTGCCGGGAATGCGGTCAGAATTCAGCCCCTTACTGACGTATGGTGTGCTGCTCCCGCTGATGGCGGAA
AAGCCTTTCCCGAGATTAAGCCCGTAATTGTCGTCCCCCTGACTTTTATAGTACTGCGTTATCAGTTGAA
GCTGCCGGGATTCATCGATGTTCAGCGTACCGGTTCCCATGATGTCCAGCCGATTGGAGTGCTGCAGGCC
GGTCTGGGTGTTATCAAGCAGGGTGGCATCGCCGTTACCGTCAAACCAGCCGCCAAATTTCTGATATGCC
ACGGAAAGACGTCCGGAGATATGGTCATTTCCGCCGGAGACAGCACCGGCAATGCGCTCATCGTGATCTT
TACTGCTGTTAAAGCCACTTTTTGTGCCAGCCTCAAACTCCATCATGGTTTCCGGCTGGCCTTTTTTGGT
CACGATGTTGATCAACCCTCCGGTACTCCCGCCACCGTACAGGGCCGTCGCGCCGGAGATCACTTCAATA
TGGTCGATATTAAAAGGATCGACAGAGTCCAGTTGTCGGCTGTCGGAACGTGAAGAGTTGAGGCGCACAC
CGTCAATCAGGACAACCAGCGGGCGGCCACGCATGTTCATACCGTAGTTGGTTCGGCTCTGGCTGCTGAC
ATCAAGGCCGGGGATTAACTGAGCCAGTGCGTCTTTCAGCTCTTTACCGCCCTGAATCTGCTGCTCCAGT
TCGGCATTTTCGATAACCCAGGTGGTTTGCGCCATCTCCGCTACAGTTCGATTGCTGCGGCTGGCAGACA
CTATGATCTCATTATCATCGTTTTGCTGGGCGACAGCTGGTGACAGCACGACAAGAAGCAGCGGACCAAG
AGCCCGGGGCATATACTTTTTGCGCATCATCGTTATTCCTGAATATTTGTTGTATAGTGAGTTAAAGCAG
CAGCCTCCGGCTGCGGTTTTTTCCGGCTGCCGCTGCGCCA

Protein Information

Protein Name

TonB-dependent receptor

NCBI Protein GI

WP_000079029

Protein Accession

NP_755498

Protein pI

5.01

Protein Weight

80196.59

Protein Length

830

Protein Note

TonB-dependent siderophore receptor; TIGR01783

Protein Sequence

>WP_000079029.1 TonB-dependent receptor [Escherichia coli]
MAQRQPEKTAAGGCCFNSLYNKYSGITMMRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTV
AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLIDGVRLNSSRSD
SRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMMEFEAGTKSGFNSSKDHDERIAGA
VSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQTGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKS
QGDDNYGLNLGKGFSAISGSSTPYVSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFY
PFPTVNANKQATAFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQKIAAGKAISADAI
PGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRGIYGAAVNGHLPLTKSVNVSDSKLE
GVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVERNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGV
NFNVLKTESKVNGQWQKYDVKESSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISS
WQLPVGTLSFSVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF

Vaxign Prediction

Localization(Probability)

Outer Membrane
(Prob.=1)

Adhesin Probability

0.612

Trans-membrane Helices

Detailed Vaxign Results

Vaxign Results

Epitope Information

IEDB Linear Epitope